SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_026596328.1 NCBI__GCF_000421465.1:WP_026596328.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
65% identity, 99% coverage: 10:1267/1272 of query aligns to 2:1224/1227 of P13009

query
sites
P13009

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C247 (= C252) binding Zn(2+)
C310 (= C315) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
C311 (= C316) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
E694 (= E735) binding methylcob(III)alamin
GDVHD 756:760 (= GDVHD 797:801) binding methylcob(III)alamin
D757 (= D798) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
H759 (= H800) binding axial binding residue; mutation to G: Loss of catalytic activity.
S804 (= S845) binding methylcob(III)alamin
T808 (= T849) binding methylcob(III)alamin
S810 (= S851) mutation to A: Decreases activity by about 40%.
A860 (= A901) binding methylcob(III)alamin
D946 (= D988) binding S-adenosyl-L-methionine
R1134 (= R1177) binding S-adenosyl-L-methionine
YY 1189:1190 (≠ YF 1232:1233) binding S-adenosyl-L-methionine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
53% identity, 99% coverage: 13:1267/1272 of query aligns to 22:1262/1265 of Q99707

query
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R61 (≠ S52) natural variant: R -> K
C255 (≠ S247) to Y: in dbSNP:rs1140598
GSR 382:384 (≠ GSA 410:412) binding (6S)-5,6,7,8-tetrahydrofolate
D449 (= D477) binding (6S)-5,6,7,8-tetrahydrofolate
N470 (= N498) binding (6S)-5,6,7,8-tetrahydrofolate
D537 (= D565) binding (6S)-5,6,7,8-tetrahydrofolate
N579 (= N607) binding (6S)-5,6,7,8-tetrahydrofolate
R585 (= R613) binding (6S)-5,6,7,8-tetrahydrofolate
R591 (= R619) binding (6S)-5,6,7,8-tetrahydrofolate
D919 (≠ R934) to G: in dbSNP:rs1805087
D963 (= D977) mutation to E: Decreases binding to MTRR; when associated with N-1071.
K1071 (= K1077) mutation to N: Decreases binding to MTRR; when associated with E-963.

3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
63% identity, 45% coverage: 694:1267/1272 of query aligns to 3:574/576 of 3ivaA

query
sites
3ivaA

E

E

W

W

R

R

T

S

W

W

P

E

V

V

E

N

K

K

R

R

L

L

E

E

H

Y

A

S

L

L

V

V

K

K

G

G

I

I

T

T

D

E

Y

F

I

I

D

E

E

Q

D

D

T

T

E

E

E

E

A

A

R

R

Q

Q

D

Q

L

A

G

T

N

R

P

P

L

C

R

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

A

D

G

G

M

M

N

N

V

V

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

K

Q

A

A

V

V

A

A

Y

Y

L

L

M

E

P

P

Y

F

L

I

D

E

A

A

E

S

R

K

Q

E

A

Q

G

C

E

K

T

T

A

N

G

G

R

K

I

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G
|
G

K

K

N

N

I

I

V
|
V

G

G

V

V

V

V

L

L

Q

Q

C

C

N

N

G

N

F

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

A

A

D

E

Q

K

I

I

L

L

Q

R

T

T

A

A

R

K

E

E

K

V

K

N

V

A

D

D

I

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

H

N

V

V

A

A

K

K

E

E

M

M

K

E

R

R

Q

Q

G

G

F

F

E

T

I

I

P

P

L

L

L

L

I

I

G
|
G

G
|
G

A

A

T

T

T

T

S

S

R

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

A

E

P

Q

H

N

Y

Y

S

S

K

G

P

P

V

T

V

V

Y

Y

V

V

S
x
Q

D
x
N

A

A

S

S

R

R

A

T

V

V

G

G

V

V

A

V

G

A

N

A

L

L

L

L

S

S

K

D

D

T

L

Q

R

R

T

D

A

D

Y

F

V

V

E

A

S

R

L

T

A

R

Q

K

E

E

Y

Y

E

E

E

T

V

V

R

R

R

I

R

Q

H

H

A

G

M

R

K

K

Q

K

D

P

R

R

T

T

R

P

L

P

L

V

S

T

I

L

E

E

A

A

A

A

R

R

N

D

N

N

R

D

Y

F

R

A

D

F

T

D

W

W

A

Q

D

A

Y

Y

E

T

P

P

P

P

P

V

P

A

I

H

E

R

A

L

G

G

L

V

Q

Q

V

E

F

V

D

E

D

A

Y

-

P

S

L

I

D

E

E

T

L

L

T

R

R

N

Y

Y

I

I

D

D

W

W

S

T

P

P

F

F

F

F

Q

M

A
x
T

W

W

E

S

L

L

A

A

G

G

S

K

Y

Y

P

P

K

R

I

I

L

L

E

E

D

D

E

E

V

V

V

V

G

G

E

V

E

E

A

A

R

Q

R

R

L

L

F

F

A

K

D

D

A

A

R

N

Q

D

M

M

L

L

E

D

Q

K

L

L

V

S

A

A

E

E

K

K

W

T

L

L

E

N

A

P

R

R

G

G

V

V

I

V

G

G

L

L

F

F

P

P

A

A

A

N

S

R

R

V

G

G

D

D

D

D

I

I

V

E

I

I

Y

Y

T

R

D

D

E

E

S

T

R

R

R

T

E

H

V

V

R

I

T

N

V

V

L

S

H

H

H

H

L

L

R

R

Q

Q

Q

Q

H

T

Q

E

K

K

P

-

Q

T

G

G

Q

F

P

A

N

N

Y

Y

C

C

L

L

A

A

D

D

Y

F

V

V

A

A

P

P

E

K

A

L

A

S

D

G

R

K

A

A

D

D

W

Y

I

I

G

G

A

A

F

F

A

A

V

V

T

T

A

G

G

G

I

L

G

E

I

E

E

D

P

A

H

L

L

A

E

D

R

A

F

F

E

E

K

A

D

Q

H

H

D

D

D

D

Y

Y

R

N

A

K

I

I

M

M

L

V

K

K

A

A

L

L

A

A

D
|
D

R

R

L

L

A

A

E
|
E

A

A

F

F

A

A

E

E

H

Y

L

L

H

H

Q

E

R

R

V

V

R

R

R

K

E

V

F

Y

W

W

G

G

Y

Y

A

A

A

P

D

N

E

E

N

N

W

L

A

S

N

N

A

E

D

E

L

L

I

I

A

R

E

E

K

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G
|
G

Y
|
Y

P

P

A
|
A

C

C

P

P

D

E

H
|
H

T

T

E

E

K

K

H

A

T

T

L

I

F

W

D

E

L

L

I

L

S

E

P

V

E

E

E

K

N

H

A

T

G

G

I

M

E

K

L

L

T

T

E

E

S

S

F

F

A
|
A

M
|
M

Y

W

P

P

A
x
G

A

A

A

S

V
|
V

S
|
S

G

G

W

W

Y

Y

F

F

S

S

H

H

P

P

R

D

A

S

S

K

Y
|
Y

F

Y

N

A

V

V

G

A

K

Q

I

I

G

Q

R

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

R

R

R

R

K

K

G

G

V

M

S

S

T

V

E

T

Q

E

V

V

E

E

K

R

W

W

L

L

A

A

P

P

N

N

L

L

A

G

Y

Y

active site: D107 (= D798), H109 (= H800), S160 (= S851)
binding cobalamin: H109 (= H800), G112 (= G803), V116 (= V807), G152 (= G843), L153 (= L844), S154 (= S845), L156 (= L847), I157 (= I848), T158 (= T849), G183 (= G874), G184 (= G875), Q208 (≠ S899), N209 (≠ D900), T303 (≠ A995), D443 (= D1136), A486 (= A1179), G488 (= G1181), Y489 (= Y1182), H495 (= H1188), A520 (= A1213), M521 (= M1214), G524 (≠ A1217), V527 (= V1220), S528 (= S1221)
binding s-adenosyl-l-homocysteine: E447 (= E1140), R484 (= R1177), P485 (= P1178), Y489 (= Y1182), A491 (= A1184), Y539 (= Y1232)

3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
63% identity, 45% coverage: 694:1267/1272 of query aligns to 3:574/577 of 3bulA

query
sites
3bulA

E

E

W

W

R

R

T

S

W

W

P

E

V

V

E

N

K

K

R

R

L

L

E

E

H

Y

A

S

L

L

V

V

K

K

G

G

I

I

T

T

D

E

Y

F

I

I

D

E

E

Q

D

D

T

T

E

E

E

E

A

A

R

R

Q

Q

D

Q

L

A

G

T

N

R

P

P

L

C

R

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

A

D

G

G

M

M

N

N

V

V

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

K

Q

A

A

V

V

A

A

Y

Y

L

L

M

E

P

P

Y

F

L

I

D

E

A

A

E

S

R

K

Q

E

A

Q

G

C

E

K

T

T

A

N

G

G

R

K

I

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G

G

K

K

N

N

I

I

V
|
V

G

G

V

V

V

V

L

L

Q

Q

C

C

N

N

G

N

F

Y

E

E

V

I

V

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

A

A

D

E

Q

K

I

I

L

L

Q

R

T

T

A

A

R

K

E

E

K

V

K

N

V

A

D

D

I

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

V

V

H

N

V

V

A

A

K

K

E

E

M

M

K

E

R

R

Q

Q

G

G

F

F

E

T

I

I

P

P

L

L

L

L

I

I

G
|
G

G
|
G

A

A

T

T

T

T

S

S

R

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

A

E

P

Q

H

N

Y

Y

S

S

K

G

P

P

V

T

V

V

Y

Y

V

V

S
x
Q

D
x
N

A
|
A

S

S

R

R

A
x
T

V

V

G

G

V

V

A

V

G

A

N

A

L

L

L

L

S

S

K

D

D

T

L

Q

R

R

T

D

A

D

Y

F

V

V

E

A

S

R

L

T

A

R

Q

K

E

E

Y

Y

E

E

E

T

V

V

R

R

R

I

R

Q

H

H

A

G

M

R

K

K

Q

K

D

P

R

R

T

T

R

P

L

P

L

V

S

T

I

L

E

E

A

A

A

A

R

R

N

D

N

N

R

D

Y

F

R

A

D

F

T

D

W

W

A

Q

D

A

Y

Y

E

T

P

P

P

P

P

V

P

A

I

H

E

R

A

L

G

G

L

V

Q

Q

V

E

F

V

D

E

D

A

Y

-

P

S

L

I

D

E

E

T

L

L

T

R

R

N

Y

Y

I

I

D

D

W

W

S

T

P

P

F

F

F

F

Q
x
M

A

T

W

W

E

S

L

L

A

A

G

G

S

K

Y

Y

P

P

K

R

I

I

L

L

E

E

D

D

E

E

V

V

V

V

G

G

E

V

E

E

A

A

R

Q

R

R

L

L

F

F

A

K

D

D

A

A

R

N

Q

D

M

M

L

L

E

D

Q

K

L

L

V

S

A

A

E

E

K

K

W

T

L

L

E

N

A

P

R

R

G

G

V

V

I

V

G

G

L

L

F

F

P

P

A

A

A

N

S

R

R

V

G

G

D

D

D

D

I

I

V

E

I

I

Y

Y

T

R

D

D

E

E

S

T

R

R

R

T

E

H

V

V

R

I

T

N

V

V

L

S

H

H

H

H

L

L

R

R

Q

Q

Q

Q

H

T

Q

E

K

K

P

-

Q

T

G

G

Q

F

P

A

N

N

Y

Y

C

C

L

L

A

A

D

D

Y

F

V

V

A

A

P

P

E

K

A

L

A

S

D

G

R

K

A

A

D

D

W

Y

I

I

G

G

A

A

F

F

A

A

V

V

T

T

A

G

G

G

I

L

G

E

I

E

E

D

P

A

H

L

L

A

E

D

R

A

F

F

E

E

K

A

D

Q

H

H

D

D

D

D

Y

Y

R

N

A

K

I

I

M

M

L

V

K

K

A

A

L

L

A

A

D
|
D

R

R

L

L

A

A

E

E

A

A

F

F

A

A

E

E

H

Y

L

L

H

H

Q

E

R

R

V

V

R

R

R

K

E

V

F

Y

W

W

G

G

Y

Y

A

A

A

P

D

N

E

E

N

N

W

L

A

S

N

N

A

E

D

E

L

L

I

I

A

R

E

E

K

N

Y

Y

R

Q

G

G

I

I

R

R

P

P

A
|
A

P
|
P

G
|
G

Y
|
Y

P

P

A

A

C

C

P

P

D

E

H
|
H

T

T

E

E

K
|
K

H

A

T

T

L

I

F

W

D

E

L

L

I

L

S

E

P

V

E

E

E

K

N

H

A

T

G

G

I

M

E

K

L

L

T

T

E

E

S

S

F

F

A

A

M
|
M

Y

W

P

P

A
x
G

A

A

A

S

V
|
V

S
|
S

G

G

W

W

Y

Y

F

F

S

S

H

H

P

P

R

D

A

S

S

K

Y

Y

F

Y

N

A

V

V

G

A

K

Q

I

I

G

Q

R

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

R

R

R

R

K

K

G

G

V

M

S

S

T

V

E

T

Q

E

V

V

E

E

K

R

W

W

L

L

A

A

P

P

N

N

L

L

A

G

Y

Y

active site: D107 (= D798), H109 (= H800), S160 (= S851)
binding cobalamin: H109 (= H800), V116 (= V807), G152 (= G843), L153 (= L844), S154 (= S845), L156 (= L847), I157 (= I848), T158 (= T849), G183 (= G874), G184 (= G875), Q208 (≠ S899), N209 (≠ D900), A210 (= A901), T213 (≠ A904), M302 (≠ Q994), D443 (= D1136), A486 (= A1179), P487 (= P1180), G488 (= G1181), Y489 (= Y1182), H495 (= H1188), K498 (= K1191), M521 (= M1214), G524 (≠ A1217), V527 (= V1220), S528 (= S1221)

4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
51% identity, 52% coverage: 13:679/1272 of query aligns to 6:611/611 of 4cczA

query
sites
4cczA

I

I

K

N

R

A

L

I

L

L

A

Q

E

K

R

R

I

I

L

M

I

V

L

L

D

D

S

G

A

G

M

M

G

G

T

T

M

M

I

I

Q

Q

N

R

R

E

R

K

L

L

S

E

E

E

A

-

D

H

Y

F

R

R

G

G

Q

Q

R

E

F

F

R

K

D

D

W

H

P

A

S

R

D

P

L

L

Q

K

G

G

N

N

N

N

D

D

L

I

L

L

S

S

L

I

T

T

Q

Q

P

P

R

D

F

V

I

I

E

Y

E

Q

I

I

H

H

R

K

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binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E404), G342 (= G410), R344 (≠ A412), N430 (= N498), M458 (= M526), D497 (= D565), G536 (= G604), S538 (= S606), N539 (= N607), F542 (= F610), R545 (= R613), R551 (= R619)

8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
36% identity, 68% coverage: 15:883/1272 of query aligns to 7:806/841 of 8g3hA

query
sites
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