SitesBLAST
Comparing WP_026607795.1 NCBI__GCF_000427445.1:WP_026607795.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
35% identity, 88% coverage: 13:262/283 of query aligns to 11:252/267 of 2hk9B
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V67 (≠ L69), G130 (= G134), G133 (= G137), A134 (= A138), N153 (= N158), R154 (= R159), T155 (= T160), K158 (≠ R163), T188 (= T196), S189 (= S197), V190 (≠ L198), I214 (= I222), M238 (= M248), L239 (= L249)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S21), S21 (= S23), N64 (= N66), T66 (= T68), K70 (= K72), N91 (= N93), D106 (= D108), Y216 (= Y224), L239 (= L249), Q242 (= Q252)
2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
35% identity, 88% coverage: 13:262/283 of query aligns to 11:252/269 of 2hk9A
- binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ L69), G132 (= G136), G133 (= G137), A134 (= A138), N153 (= N158), R154 (= R159), T155 (= T160), T188 (= T196), S189 (= S197), V190 (≠ L198)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S21), S21 (= S23), N64 (= N66), K70 (= K72), N91 (= N93), D106 (= D108), Y216 (= Y224), L239 (= L249), Q242 (= Q252)
O67049 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Aquifex aeolicus (strain VF5) (see paper)
35% identity, 88% coverage: 13:262/283 of query aligns to 11:252/269 of O67049
- SLS 19:21 (≠ SRS 21:23) binding shikimate
- D82 (≠ E84) binding NADP(+)
- N91 (= N93) binding shikimate
- D106 (= D108) binding shikimate
- GAGGA 130:134 (= GAGGA 134:138) binding NADP(+)
- I214 (= I222) binding NADP(+)
- Y216 (= Y224) binding shikimate
- G235 (= G245) binding NADP(+)
- Q242 (= Q252) binding shikimate
1nytA Shikimate dehydrogenase aroe complexed with NADP+ (see paper)
39% identity, 93% coverage: 13:276/283 of query aligns to 6:268/271 of 1nytA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K65 (= K72), D102 (= D108), G128 (= G136), G129 (= G137), A130 (= A138), N149 (= N158), R150 (= R159), T151 (= T160), R154 (= R163), T188 (= T196), S189 (= S197), S190 (≠ L198), M213 (≠ I222), G237 (= G245), M240 (= M248), L241 (= L249)
P15770 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Escherichia coli (strain K12) (see paper)
39% identity, 93% coverage: 13:276/283 of query aligns to 6:268/272 of P15770
Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
33% identity, 91% coverage: 10:267/283 of query aligns to 2:254/269 of Q5HNV1
- SLS 13:15 (≠ SRS 21:23) binding shikimate
- T60 (= T68) binding shikimate
- N85 (= N93) binding shikimate
- D100 (= D108) binding shikimate
- Y211 (= Y224) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
- Q239 (= Q252) binding shikimate
Q9KVT3 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
34% identity, 93% coverage: 13:274/283 of query aligns to 10:270/278 of Q9KVT3
- SKS 18:20 (≠ SRS 21:23) binding shikimate
- N90 (= N93) binding shikimate
- D106 (= D108) binding shikimate
- NRTFAK 154:159 (≠ NRTKDR 158:163) binding NADP(+)
- Q248 (= Q252) binding shikimate
3pgjA 2.49 angstrom resolution crystal structure of shikimate 5- dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate
34% identity, 93% coverage: 13:274/283 of query aligns to 6:266/272 of 3pgjA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (= S21), S16 (= S23), N59 (= N66), T61 (= T68), K65 (= K72), N86 (= N93), D102 (= D108), Q244 (= Q252)
3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
32% identity, 91% coverage: 10:267/283 of query aligns to 2:245/258 of 3dooA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (= S21), S15 (= S23), N58 (= N66), T60 (= T68), K64 (= K72), N85 (= N93), D100 (= D108), F227 (≠ L249), Q230 (= Q252)
3sefC 2.4 angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate and NADPH
33% identity, 93% coverage: 13:274/283 of query aligns to 6:239/244 of 3sefC
Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
31% identity, 93% coverage: 13:276/283 of query aligns to 11:278/282 of Q58484
1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
31% identity, 93% coverage: 13:276/283 of query aligns to 16:283/287 of 1nvtB
- active site: K75 (= K72), D111 (= D108)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (≠ L69), G135 (≠ A135), G137 (= G137), G138 (≠ A138), A139 (= A139), N157 (= N158), R158 (= R159), T159 (= T160), K162 (≠ R163), A200 (≠ T195), T201 (= T196), P202 (≠ S197), I203 (≠ L198), M205 (= M200), L229 (≠ I222), Y231 (= Y224), M255 (= M248), L256 (= L249)
- binding zinc ion: E22 (≠ G19), H23 (= H20)
1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
31% identity, 93% coverage: 13:276/283 of query aligns to 16:283/287 of 1nvtA
- active site: K75 (= K72), D111 (= D108)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (≠ A135), A139 (= A139), N157 (= N158), R158 (= R159), T159 (= T160), K162 (≠ R163), A200 (≠ T195), T201 (= T196), P202 (≠ S197), I203 (≠ L198), M205 (= M200), L229 (≠ I222), Y231 (= Y224), G252 (= G245), M255 (= M248), L256 (= L249)
2ev9B Crystal structure of shikimate 5-dehydrogenase (aroe) from thermus thermophilus hb8 in complex with NADP(h) and shikimate (see paper)
41% identity, 90% coverage: 10:265/283 of query aligns to 3:248/263 of 2ev9B
- active site: K64 (= K72), D100 (= D108)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (= S21), S16 (= S23), N58 (= N66), T60 (= T68), K64 (= K72), N85 (= N93), D100 (= D108), Q235 (= Q252)
Q5SJF8 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
41% identity, 90% coverage: 10:265/283 of query aligns to 3:248/263 of Q5SJF8
- SLS 14:16 (≠ SRS 21:23) binding shikimate
- T60 (= T68) binding shikimate
- K64 (= K72) active site, Proton acceptor
- N85 (= N93) binding shikimate
- D100 (= D108) binding shikimate
- GAGGA 123:127 (= GAGGA 134:138) binding NADP(+)
- NRTPQR 146:151 (≠ NRTKDR 158:163) binding NADP(+)
- L205 (≠ I222) binding NADP(+)
- Y207 (= Y224) binding shikimate
- G228 (= G245) binding NADP(+)
- Q235 (= Q252) binding shikimate
2cy0A Crystal structure of shikimate 5-dehydrogenase (aroe) from thermus thermophilus hb8 in complex with NADP (see paper)
41% identity, 90% coverage: 10:265/283 of query aligns to 3:248/262 of 2cy0A
- active site: K64 (= K72), D100 (= D108)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G123 (= G134), G126 (= G137), A127 (= A138), N146 (= N158), R147 (= R159), T148 (= T160), R151 (= R163), T179 (= T196), R180 (≠ S197), V181 (≠ L198), L205 (≠ I222), L232 (= L249)
3sefA 2.4 angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate and NADPH
34% identity, 93% coverage: 13:274/283 of query aligns to 6:262/268 of 3sefA