SitesBLAST
Comparing WP_026608290.1 NCBI__GCF_000427445.1:WP_026608290.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8sadA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp/malonate complex (c2 form)
44% identity, 95% coverage: 16:391/395 of query aligns to 11:397/398 of 8sadA
- binding magnesium ion: A359 (≠ R353), R362 (= R356), A365 (≠ T359)
- binding pyridoxal-5'-phosphate: C88 (≠ S88), G89 (= G89), A90 (≠ L90), Y114 (= Y114), D188 (= D186), A210 (= A208), T212 (= T210), K213 (= K211), M222 (≠ L220), W343 (= W340)
8u99A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-serine adduct)
44% identity, 95% coverage: 16:391/395 of query aligns to 4:390/391 of 8u99A
- binding pyridoxal-5'-phosphate: C81 (≠ S88), G82 (= G89), A83 (≠ L90), Y107 (= Y114), D181 (= D186), T205 (= T210), K206 (= K211), M215 (≠ L220), W336 (= W340)
- binding serine: Y107 (= Y114), K206 (= K211), Y334 (≠ F338), S335 (= S339), W336 (= W340), R368 (= R369)
8u98A Crystal structure of cystathionine beta lyase from klebsiella aerogenes (plp-glycine adduct)
44% identity, 95% coverage: 16:391/395 of query aligns to 4:390/391 of 8u98A
- binding glycine: Y107 (= Y114), K206 (= K211), Y334 (≠ F338), S335 (= S339), W336 (= W340), R368 (= R369)
- binding pyridoxal-5'-phosphate: Y52 (= Y59), R54 (≠ T61), C81 (≠ S88), G82 (= G89), A83 (≠ L90), Y107 (= Y114), D181 (= D186), A203 (= A208), T205 (= T210), K206 (= K211), M215 (≠ L220), W336 (= W340)
8sa9A Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp-oxamate adduct (c2 form)
44% identity, 95% coverage: 16:391/395 of query aligns to 4:390/391 of 8sa9A
- binding pyridoxal-5'-phosphate: C81 (≠ S88), G82 (= G89), A83 (≠ L90), Y107 (= Y114), D181 (= D186), A203 (= A208), T205 (= T210), K206 (= K211), M215 (≠ L220), W336 (= W340)
- binding [({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino](oxo)acetic acid: C81 (≠ S88), G82 (= G89), A83 (≠ L90), Y107 (= Y114), D181 (= D186), A203 (= A208), T205 (= T210), K206 (= K211), M215 (≠ L220), S335 (= S339), W336 (= W340), R368 (= R369)
8sabA Crystal structure of cystathionine beta lyase from klebsiella aerogenes, plp adduct with alanine (c2 form)
44% identity, 95% coverage: 16:391/395 of query aligns to 5:391/392 of 8sabA
- binding lysine: N9 (≠ H20), R12 (= R23), R13 (= R24), K14 (≠ P25), T17 (≠ Q28), L330 (= L333), E341 (= E344)
- binding pyridoxal-5'-phosphate: C82 (≠ S88), G83 (= G89), A84 (≠ L90), Y108 (= Y114), D182 (= D186), A204 (= A208), T206 (= T210), K207 (= K211), M216 (≠ L220), W337 (= W340)
- binding alanyl-pyridoxal-5'-phosphate: C82 (≠ S88), G83 (= G89), A84 (≠ L90), Y108 (= Y114), D182 (= D186), A204 (= A208), T206 (= T210), K207 (= K211), M216 (≠ L220), Y335 (≠ F338), S336 (= S339), W337 (= W340), R369 (= R369)
2gqnA Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-nitro-benzamide (see paper)
41% identity, 96% coverage: 13:391/395 of query aligns to 1:390/391 of 2gqnA
- active site: R54 (≠ T61), Y107 (= Y114), D181 (= D186), K206 (= K211)
- binding (5-hydroxy-6-methyl-4-((2-(2-(2-nitrobenzamido)acetyl)hydrazinyl)methyl)pyridin-3-yl)methyl dihydrogen phosphate: C81 (≠ S88), G82 (= G89), A83 (≠ L90), Y107 (= Y114), E108 (≠ R115), D181 (= D186), A203 (= A208), T205 (= T210), K206 (= K211), M215 (≠ L220), Y334 (≠ F338), S335 (= S339), W336 (= W340), R368 (= R369)
2fq6A Cystathionine beta-lyase (cbl) from escherichia coli in complex with n-hydrazinocarbonylmethyl-2-trifluoromethyl-benzamide (see paper)
41% identity, 96% coverage: 13:391/395 of query aligns to 1:390/391 of 2fq6A
- active site: R54 (≠ T61), Y107 (= Y114), D181 (= D186), K206 (= K211)
- binding phosphoric acid mono-(5-hydroxy-6-methyl-4-{[2-(2-trifluoromethyl-benzoylamino)-acetyl]-hydrazonomethyl}-pyridin-3-ylmethyl)ester: C81 (≠ S88), G82 (= G89), A83 (≠ L90), Y107 (= Y114), P109 (= P116), D181 (= D186), A203 (= A208), T205 (= T210), K206 (= K211), M215 (≠ L220), Y334 (≠ F338), S335 (= S339), W336 (= W340), R368 (= R369)
1cl2A Cystathionine beta-lyase (cbl) from escherichia coli in complex with aminoethoxyvinylglycine (see paper)
41% identity, 96% coverage: 13:391/395 of query aligns to 1:390/391 of 1cl2A
- active site: R54 (≠ T61), Y107 (= Y114), D181 (= D186), K206 (= K211)
- binding (2E,3E)-4-(2-aminoethoxy)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: C81 (≠ S88), G82 (= G89), A83 (≠ L90), Y107 (= Y114), D181 (= D186), A203 (= A208), T205 (= T210), K206 (= K211), M215 (≠ L220), Y334 (≠ F338), S335 (= S339), W336 (= W340), R368 (= R369)
1cl1B Cystathionine beta-lyase (cbl) from escherichia coli (see paper)
41% identity, 96% coverage: 13:391/395 of query aligns to 2:391/392 of 1cl1B
P06721 Cystathionine beta-lyase MetC; CBL; CL; Beta-cystathionase MetC; Cysteine desulfhydrase MetC; CD; Cysteine lyase MetC; Cysteine-S-conjugate beta-lyase MetC; EC 4.4.1.13; EC 4.4.1.28 from Escherichia coli (strain K12) (see 2 papers)
41% identity, 96% coverage: 13:391/395 of query aligns to 5:394/395 of P06721
- K210 (= K211) modified: N6-(pyridoxal phosphate)lysine
4itxA P113s mutant of e. Coli cystathionine beta-lyase metc inhibited by reaction with l-ala-p (see paper)
41% identity, 96% coverage: 13:391/395 of query aligns to 1:390/391 of 4itxA
- active site: R54 (≠ T61), Y107 (= Y114), D181 (= D186), K206 (= K211)
- binding {1-[(3-hydroxy-methyl-5-phosphonooxy-methyl-pyridin-4-ylmethyl)-amino]-ethyl}-phosphonic acid: C81 (≠ S88), G82 (= G89), A83 (≠ L90), Y107 (= Y114), D181 (= D186), A203 (= A208), T205 (= T210), K206 (= K211), M215 (≠ L220), Y334 (≠ F338), S335 (= S339), W336 (= W340), R368 (= R369)
4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
35% identity, 96% coverage: 12:391/395 of query aligns to 1:373/373 of 4l0oH
- active site: R40 (≠ T61), Y92 (= Y114), D164 (= D186), K189 (= K211)
- binding pyridoxal-5'-phosphate: Y38 (= Y59), R40 (≠ T61), S67 (= S88), G68 (= G89), L69 (= L90), Y92 (= Y114), D164 (= D186), S186 (≠ A208), T188 (= T210), K189 (= K211)
7d7oB Crystal structure of cystathionine gamma-lyase from bacillus cereus atcc 14579 (see paper)
34% identity, 95% coverage: 12:388/395 of query aligns to 1:376/377 of 7d7oB
P55217 Cystathionine gamma-synthase 1, chloroplastic; AtCGS1; METHIONINE OVERACCUMULATION 1; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
31% identity, 94% coverage: 19:391/395 of query aligns to 180:563/563 of P55217
- T412 (≠ C244) natural variant: T -> P
- G459 (≠ A291) natural variant: G -> A
Sites not aligning to the query:
- 8 natural variant: C -> S
- 55 natural variant: A -> G
- 77 R→H: In mto1-4; over-accumulation of soluble methionine.
- 78 R→K: In mto1-7; over-accumulation of soluble methionine.
- 81 S→N: In mto1-2; over-accumulation of soluble methionine.
- 84 G→D: In mto1-3 and mto1-5; over-accumulation of soluble methionine.; G→S: In mto1-1; over-accumulation of soluble methionine.
- 86 A→V: In mto1-6; over-accumulation of soluble methionine.
- 91 natural variant: A -> G
1qgnA Cystathionine gamma-synthase from nicotiana tabacum (see paper)
32% identity, 94% coverage: 19:391/395 of query aligns to 15:398/398 of 1qgnA
- active site: R63 (≠ T61), Y116 (= Y114), D189 (= D186), K214 (= K211)
- binding pyridoxal-5'-phosphate: Y61 (= Y59), R63 (≠ T61), S90 (= S88), G91 (= G89), M92 (≠ L90), Y116 (= Y114), D189 (= D186), S211 (≠ A208), T213 (= T210), K214 (= K211), F342 (≠ W340)
1i48A Cystathionine gamma-synthase in complex with the inhibitor ctcpo (see paper)
32% identity, 94% coverage: 19:391/395 of query aligns to 13:396/396 of 1i48A
- active site: R61 (≠ T61), Y114 (= Y114), D187 (= D186), K212 (= K211)
- binding carboxymethylthio-3-(3-chlorophenyl)-1,2,4-oxadiazol: Y114 (= Y114), R115 (= R115), A337 (≠ G337), P338 (≠ F338), S339 (= S339), D348 (≠ I348), S354 (≠ V354), R374 (= R369), S376 (= S371)
- binding pyridoxal-5'-phosphate: Y59 (= Y59), R61 (≠ T61), S88 (= S88), G89 (= G89), M90 (≠ L90), Y114 (= Y114), D187 (= D186), S209 (≠ A208), T211 (= T210), K212 (= K211), F340 (≠ W340)
1i43A Cystathionine gamma-synthase in complex with the inhibitor ppca (see paper)
32% identity, 94% coverage: 19:391/395 of query aligns to 13:396/396 of 1i43A
- active site: R61 (≠ T61), Y114 (= Y114), D187 (= D186), K212 (= K211)
- binding pyridoxal-5'-phosphate: Y59 (= Y59), R61 (≠ T61), S88 (= S88), G89 (= G89), M90 (≠ L90), Y114 (= Y114), D187 (= D186), S209 (≠ A208), T211 (= T210), K212 (= K211), F340 (≠ W340)
- binding 3-(phosphonomethyl)pyridine-2-carboxylic acid: E58 (≠ S58), Y62 (≠ Q62), Y114 (= Y114), K212 (= K211), P338 (≠ F338), S354 (≠ V354), R374 (= R369)
1i41A Cystathionine gamma-synthase in complex with the inhibitor appa (see paper)
32% identity, 94% coverage: 19:391/395 of query aligns to 13:396/396 of 1i41A
- active site: R61 (≠ T61), Y114 (= Y114), D187 (= D186), K212 (= K211)
- binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-imino]-5-phosphono-pent-3-enoic acid: E58 (≠ S58), Y59 (= Y59), R61 (≠ T61), Y62 (≠ Q62), G89 (= G89), M90 (≠ L90), Y114 (= Y114), E158 (= E157), D187 (= D186), T189 (= T188), S209 (≠ A208), T211 (= T210), K212 (= K211), P338 (≠ F338), S339 (= S339), R374 (= R369)
7ba4A Structure of cystathionine gamma-lyase from pseudomonas aeruginosa
34% identity, 95% coverage: 16:392/395 of query aligns to 9:375/377 of 7ba4A
5x5hA Crystal structure of metb from corynebacterium glutamicum (see paper)
32% identity, 95% coverage: 16:392/395 of query aligns to 10:385/385 of 5x5hA
- active site: R52 (≠ T61), Y105 (= Y114), D177 (= D186), K202 (= K211)
- binding magnesium ion: F36 (≠ V42), Q38 (≠ F44), E49 (≠ S58), E69 (≠ S78), Q187 (≠ P196), P188 (= P197), L189 (≠ H198)
- binding pyridoxal-5'-phosphate: G80 (= G89), M81 (≠ L90), Y105 (= Y114), D177 (= D186), S199 (≠ A208), T201 (= T210), K202 (= K211)
Query Sequence
>WP_026608290.1 NCBI__GCF_000427445.1:WP_026608290.1
MTETTPDDRKGLKAKTRLIHAGRRPSEQFGFVNTPIYRGSTVLFPTLDDLTRRNAKFSYG
TQGTPTTEALETAWTELSGAAGTVLVPSGLAAISLALLTAVKAGDHILVTDSAYRPTRIF
CDGILARMGVETTYYDPWIGAGIEALIRANTSVIFLEAPGSQSFEMQDVPAIAKVARAKG
VCTILDNTWATPIFFPPHERGVDMAIEAGTKYLSGHSDLLLGLVSANAQWLNRLRATFDA
FAMCPGPEDVFLALRGLRTLDLRLREAERQGLALATWLAERDEVAVVLHPALPSCPGHAL
WKRDFLGSSGLFSVILRPCSTAALAAMLDGLKLFGMGFSWGGFESLVIPFDCRVYRTATQ
WSPPGPALRFSVGLEDIEDLKADLAEGFERLRAVG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory