SitesBLAST

K3849 (= K63) mutation to M: Does not affect intracellular vesicle localization. Affects cholesterol internalization.
T3999 (≠ S215) mutation to A: Does not affect protein expression. Affects intracellular vesicles localization. Impairs intracellular cholesterol accumulation in the vesicle.

Sites not aligning to the query:

3577 H→P: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.
4735 K→M: Does not affect intracellular vesicle localization.Affects cholesterol internalization.
4818 R→C: Does not affect protein expression. Decreases intracellular cholesterol accumulation in the vesicle.

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
36% identity, 41% coverage: 24:234/513 of query aligns to 1:217/343 of P30750

query
sites
P30750

I

M

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N

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G

40:46 (vs. 59:65, 86% identical) binding ATP
E166 (= E184) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
36% identity, 41% coverage: 24:234/513 of query aligns to 2:218/344 of 6cvlD

query
sites
6cvlD

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M

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G

binding phosphothiophosphoric acid-adenylate ester: F12 (= F34), Q14 (≠ K36), I19 (≠ V37), S41 (≠ N59), G42 (= G60), A43 (= A61), G44 (= G62), K45 (= K63), S46 (= S64), T47 (= T65), N141 (≠ Q158), S143 (= S160), Q146 (≠ E163), H200 (= H216)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
36% identity, 41% coverage: 24:234/513 of query aligns to 2:218/344 of 3tuzC

query
sites
3tuzC

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G

binding adenosine-5'-diphosphate: F12 (= F34), Q14 (≠ K36), G42 (= G60), G44 (= G62), K45 (= K63), S46 (= S64), T47 (= T65)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
36% identity, 41% coverage: 24:234/513 of query aligns to 2:218/344 of 3tuiC

query
sites
3tuiC

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M

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G

binding adenosine-5'-diphosphate: F12 (= F34), G42 (= G60), A43 (= A61), G44 (= G62), K45 (= K63), S46 (= S64), T47 (= T65)

O95477 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; Cholesterol efflux regulatory protein; EC 7.6.2.1 from Homo sapiens (Human) (see 35 papers)
33% identity, 42% coverage: 25:240/513 of query aligns to 899:1116/2261 of O95477

query
sites
O95477

V

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M

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L

D917 (≠ N41) to Y: in a colorectal cancer sample; somatic mutation
T929 (≠ K53) to I: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane; dbSNP:rs1832457117
N935 (= N59) to S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs28937313
K939 (= K63) mutation to M: Inhibits ATPase activity; when associated with M-1952. Decreases translocase activity; when associated with M-1952. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-1952.
S1042 (≠ E168) modified: Phosphoserine; by PKA
P1065 (= P191) natural variant: P -> S
M1091 (≠ L218) to T: in FHA1; loss of localization to plasma membrane; decreased cholesterol efflux; decreased phospholipid efflux
C1110 (vs. gap) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1111.
C1111 (= C235) modified: S-palmitoyl cysteine; mutation to S: Decreased palmitoylation; when associated with S-3, S-23 and S-1110.

Sites not aligning to the query:

3 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased cholesterol efflux. Decreased phospholipid efflux. Decreased palmitoylation; when associated with S-23, S-1110 and S-1111.
23 modified: S-palmitoyl cysteine; C→S: Mild decrease of palmitoylation. Loss of localization to plasma membrane. Decreased palmitoylation; when associated with S-3, S-1110 and S-1111.
74 I→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.; I→K: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-371.
75 modified: Disulfide link with 309
85 P → L: in FHA1; Alabama; dbSNP:rs145183203
98 modified: carbohydrate, N-linked (GlcNAc...) asparagine
100 S→C: Highly decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
210 E → D: in a colorectal cancer sample; somatic mutation
219 R → K: in dbSNP:rs2230806
230 R → C: in dbSNP:rs9282541
244 modified: carbohydrate, N-linked (GlcNAc...) asparagine
248 P → A: in dbSNP:rs142625938
255 A → T: in TGD; deficient cellular cholesterol efflux; dbSNP:rs758100110
304 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-308.
308 V→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane; when associated with C-304.
309 modified: Disulfide link with 75
364 S → C: in dbSNP:rs775035559
371 I→C: No effect on phospholipid and cholesterol efflux or localization to cell membrane. 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-74 or C-375.; I→E: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-74.
375 L→C: 85-90% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with C-371.
399 V → A: in dbSNP:rs9282543
401 K → Q: in dbSNP:rs138487227
496 R → W: in dbSNP:rs147675550
568 K→A: 60-65% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane.
573 Y→F: No effect on phospholipid and cholesterol efflux and on localization to cell membrane.
581 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-584 and K-585.
583 F→K: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with E-590.
584 E→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-585.
585 D→K: 80-85% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-581 and K-584.
590 W → S: in TGD; moderately decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; dbSNP:rs137854496; W→E: 90-95% reduction in phospholipid and cholesterol efflux but no effect on localization to cell membrane; when associated with K-583.
593 F→L: Moderately decreased protein abundance. Highly decreased ATPase activity. Highly decreased phospholipid translocase activity.
638 R → Q: in dbSNP:rs374190304
771 V → M: in dbSNP:rs2066718
774 T → P: in dbSNP:rs35819696; natural variant: T -> S
776 K → N: in dbSNP:rs138880920
815 E → G: in dbSNP:rs145582736
825 V → I: in dbSNP:rs2066715
883 I → M: in dbSNP:rs2066714
1172 E → D: in dbSNP:rs33918808
1181 S → F: in dbSNP:rs76881554
1216 G → V: in dbSNP:rs562403512
1341 R → T: in dbSNP:rs147743782
1376 S → G: in dbSNP:rs145689805
1379 L → F: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane; dbSNP:rs1831213945
1407 A → T: in a colorectal cancer sample; somatic mutation; dbSNP:rs189206655
1463 modified: Disulfide link with 1477
1477 modified: Disulfide link with 1463; C → R: in TGD; loss of interaction with APOE; unable to generate APOE-containing high density lipoproteins; moderately decreased protein abundance; moderately decreased ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs137854494
1512 T→M: Moderately decreased protein abundance. Does not affect ATPase activity. Moderately decreased phospholipid translocase activity.
1555 I → T: in dbSNP:rs1997618
1587 K → R: in dbSNP:rs2230808
1611 N → D: in FHA1; deficient cellular cholesterol efflux
1615 R → Q: in dbSNP:rs1251839800
1648 L → P: in dbSNP:rs1883024
1670 A → T: in dbSNP:rs1203589782
1680 R → Q: in dbSNP:rs150125857
1704 V → D: in TGD; the mutant protein is retained in the endoplasmic reticulum while the wild-type protein is located at the plasma membrane
1731 S → C: in dbSNP:rs760507032
1897 R → W: in FHA1; uncertain significance; dbSNP:rs760768125
1925 R → Q: in Scott syndrome; shows impaired trafficking of the mutant protein to the plasma membrane; dbSNP:rs142688906
1952 K→M: Inhibits ATPase activity; when associated with M-939. Decreases translocase activity; when associated with M-939. Does not affect protein subcellular localization in plasma membrane and endosome; when associated with M-939.
2054 modified: Phosphoserine; by PKA
2081 R → W: in TGD; highly decreased protein abundance; highly decreased ATPase activity; highly decreased phospholipid translocase activity; loss protein subcellular localization to the plasma membrane; dbSNP:rs137854501
2109 A → T: in a colorectal cancer sample; somatic mutation
2150 P → L: in FHA1; moderately decreased protein abundance; does not affect ATPase activity; moderately decreased phospholipid translocase activity; dbSNP:rs369098049
2163 natural variant: F -> S
2168 L → P: in dbSNP:rs2853577
2243 D → E: in dbSNP:rs34879708
2244 V → I: in dbSNP:rs144588452

P41233 Phospholipid-transporting ATPase ABCA1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; ABC-1; ATP-binding cassette 1; EC 7.6.2.1 from Mus musculus (Mouse) (see paper)
33% identity, 42% coverage: 25:240/513 of query aligns to 899:1116/2261 of P41233

query
sites
P41233

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Sites not aligning to the query:

489 modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9BZC7 ATP-binding cassette sub-family A member 2; ATP-binding cassette transporter 2; ATP-binding cassette 2; EC 7.6.2.- from Homo sapiens (Human) (see paper)
31% identity, 42% coverage: 24:240/513 of query aligns to 989:1206/2435 of Q9BZC7

query
sites
Q9BZC7

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Sites not aligning to the query:

271 modified: N5-methylglutamine; Q→R: Abolishes methylation by N6AMT1.

7tbwA The structure of atp-bound abca1 (see paper)
32% identity, 42% coverage: 25:240/513 of query aligns to 754:971/1928 of 7tbwA

query
sites
7tbwA

V

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binding adenosine-5'-triphosphate: Y763 (≠ F34), D765 (vs. gap), K768 (≠ V37), N790 (= N59), G791 (= G60), G793 (= G62), K794 (= K63), T795 (≠ S64), Q835 (= Q106), Q887 (= Q158), S889 (= S160), H944 (= H216)
binding magnesium ion: Q835 (= Q106), D912 (= D183), Q913 (≠ E184)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1630, 1633, 1657, 1658, 1660, 1661, 1662, 1663, 1702, 1753, 1755, 1756, 1757, 1779, 1783, 1811
binding cholesterol: 218, 219, 360, 367
binding magnesium ion: 1662, 1702

E9Q876 Glucosylceramide transporter ABCA12; ATP-binding cassette sub-family A member 12; EC 7.6.2.1 from Mus musculus (Mouse) (see 2 papers)
32% identity, 41% coverage: 25:234/513 of query aligns to 1346:1553/2595 of E9Q876

query
sites
E9Q876

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1388:1461 (vs. 67:141, 29% identical) mutation to M: In a mouse model for harlequin ichthyosis (HI), smooth skin (smsk) mutant mice show a pronounced perinatal lethal skin phenotype in 25% of the offspring and newborn mutant pups die within a few hours after birth, and appear severely dehydrated with dry cracking skin. Smsk homozygous mutants embryos show a normal appearance at 14.5 dpc, but at 16.5 dpc develop a partial absence of normal skin folds around the trunk and limbs, and by 18.5 dpc develop a taut, thick skin and limb contractures.

Sites not aligning to the query:

1996 G→D: In a mouse model for harlequin ichthyosis (HI), homozygous mice are embryonic lethal but occasionally pups are found in the first few hours after birth but die and are severely dehydrated and fail to suckle normally. Homozygous pups show hallmarks of HI desease including hyperkeratosis, abnormal extracellular lipid lamellae and defects in cornified envelope processing. At 14.5 dpc and 15.5 dpc homozygous embryos appear normal; however from 16.5 dpc onwards they are characterized by an absence of normal skin folds around the trunk and limbs. As development progressed, embryos develop a taut, thick epidermis and multiple contractures affecting the limbs. Late stage embryos are smaller.

7roqA Alternative structure of human abca1
32% identity, 39% coverage: 43:240/513 of query aligns to 790:986/1831 of 7roqA

query
sites
7roqA

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Y

Y

P

E

G

G

R

Q

I

I

K

T

A

S

L

F

L

L

G

G

E

H

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

M

M

S

S

M

I

L

L

A

T

G

G

R

L

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F

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P

P

D

T

E

S

G

G

H

T

I

A

E

Y

V

I

D

L

G

G

K

K

R

-

V

-

D

D

F

I

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R

S

S

S

E

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M

D

S

A

T

I

I

N

R

A

Q

G

N

I

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G

G

M

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C

Y

P

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Q

H

H

F

N

M

V

L

L

V

F

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D

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M

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T

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V

T

E

E

E

N

H

V

I

L

W

L

-

G

-

Q

-

E

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G

-

F

F

F

Y

V

A

N

R

P

L

K

K

E

G

M

L

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S

R

E

R

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-

H

-

V

-

K

-

A

-

E

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M

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M

A

A

E

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D

Y

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A

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Y

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E

G

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K

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I

F

L

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D

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E

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P

T

T

A

A

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G

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Y

E

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T

R

F

R

R

G

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F

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E

E

A

L

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L

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-

A

L

M

K

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Y

R

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Q

Q

G

G

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I

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I

F

L

I

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S

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H

H

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A

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A

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A

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G

G

-

K

-

L

-

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C

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E

S

V

L

Sites not aligning to the query:

binding cholesterol: 688, 1106, 1426, 1526, 1527

7tbyA The structure of human abca1 in nanodisc (see paper)
33% identity, 39% coverage: 43:240/513 of query aligns to 701:898/1788 of 7tbyA

query
sites
7tbyA

I

L

V

A

L

L

D

N

L

F

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Y

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F

L

L

G

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N

N

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A

A

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T

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M

M

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M

I

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F

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D

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E

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G

G

H

T

I

A

E

Y

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I

D

L

G

G

K

K

R

-

V

-

D

D

F

I

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E

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M

D

S

A

T

I

I

N

R

A

Q

G

N

I

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G

G

M

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V

C

Y

P

Q

Q

H

H

F

N

M

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D

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M

M

L

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T

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V

T

E

E

E

N

H

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W

L

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G

-

Q

-

E

-

G

-

F

F

F

Y

V

A

N

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P

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K

K

E

G

M

L

S

S

-

E

R

K

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H

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V

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K

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A

L

E

A

M

E

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Q

Y

M

E

A

L

L

E

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A

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I

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K

-

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S

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Q

L

L

S

S

M

G

G

G

E

M

K

Q

Q

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V

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E

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I

V

L

A

K

L

L

A

L

F

Y

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G

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S

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K

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V

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V

I

I

F

L

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D

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E

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A

A

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W

E

E

A

L

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L

W

-

A

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Q

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G

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I

I

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I

F

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H

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M

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D

E

E

V

A

M

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A

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A

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D

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R

V

I

A

A

I

I

L

I

R

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R

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G

G

-

K

-

L

-

C

C

C

V

V

D

G

S

S

E

S

V

L

Sites not aligning to the query:

binding cholesterol: 161, 162

Q8R420 Phospholipid-transporting ATPase ABCA3; ATP-binding cassette sub-family A member 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Mus musculus (Mouse) (see paper)
32% identity, 45% coverage: 10:240/513 of query aligns to 510:748/1704 of Q8R420

query
sites
Q8R420

P

P

E

E

S

K

G

A

S

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A

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G

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I

-

K

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I

K

K

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F

L

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K

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F

-

Q

A

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N

G

N

N

K

K

-

D

-

K

-

M

-

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-

I

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-

D

I

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N

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Y

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A

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L

G

G

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N

N

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A

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K

K

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M

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G

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K

Y

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-

F

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A

A

Q

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A

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M

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Y

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M

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A

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A

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Y

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A

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R

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C

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Sites not aligning to the query:

292 E→V: Knockin new born mice are healthy and survive into adulthood without overt signs of respiratory distress. Knockin mice show a severe lung phenotype that begins with alveolar inflammatory cell infiltration at the early stage of the mouse life followed by aberrant lung remodeling with characteristics of diffuse parenchymal lung disease (DPLD)- and emphysema-like alveolar disruption in older mice.

8eopA Cryo-em structure of nanodisc reconstituted human abca7 eq mutant in atp bound closed state (see paper)
31% identity, 45% coverage: 11:240/513 of query aligns to 634:866/1687 of 8eopA

query
sites
8eopA

E

E

S

A

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Y

A

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I

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I

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T

E

K

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F
|
F

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-

G

G
x
S

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A

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A

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N

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G

A

A

G
|
G

K
|
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x
T

T
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T

L

T

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M

I

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L

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-

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-

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D

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A

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D

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M

M

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E

N

H

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V

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W

L

-

G

-

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-

E

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-

F

F

F

Y

V

G

N

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P

L

K

K

E

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M

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E

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Y

V

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G

L

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x
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M

K

Q

Q

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K

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I

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L

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K

I

L

A

L

F

Y

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V

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I

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D

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P

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T

A

A

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R

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E

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binding adenosine-5'-triphosphate: F659 (= F34), S662 (≠ G35), A666 (= A39), N686 (= N59), G687 (= G60), G689 (= G62), K690 (= K63), T691 (≠ S64), T692 (= T65), H782 (≠ Q158), S784 (= S160)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 1461, 1488, 1489, 1491, 1492, 1494, 1533, 1584, 1586
binding magnesium ion: 1493, 1533

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
30% identity, 44% coverage: 23:248/513 of query aligns to 1:226/241 of 4u00A

query
sites
4u00A

P

P

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I

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F

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A
x
V

N

L

N

K

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A

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P

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I

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G

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P

N
x
S

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G

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x
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T

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A

K

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A

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I

I

N

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A

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E

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F

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F

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H

M

M

T

T

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L

E

E

N

N

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L

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G

A

Q

-

E

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F

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N

-

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M

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M

V

S

R

R

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R

W

V

P

K

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K

E

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K

A

A

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K

Y

K

E

A

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G

I

I

-

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A

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A

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Y

A

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M

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F

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A

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A

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P

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E

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M

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G

R

E

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F

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A

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Q

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G

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M

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M

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F

V

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T

H

H

K

E

L

M

E

G

E

F

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A

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A

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E

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T

K

R

binding adenosine-5'-diphosphate: F12 (= F34), V17 (≠ A39), S37 (≠ N59), G38 (= G60), S39 (≠ A61), G40 (= G62), K41 (= K63), S42 (= S64), T43 (= T65)

8ee6A Cryo-em structure of human abca7 in pe/ch nanodiscs (see paper)
32% identity, 43% coverage: 22:240/513 of query aligns to 621:840/1808 of 8ee6A

query
sites
8ee6A

S

S

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P

I

G

V

V

S

S

L

V

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G

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E

K

K

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F
|
F

-

P

-

G

G

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K

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A
|
A

N

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N

R

K

G

I

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L

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D

D

L

F

Y

Y

P

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G

G

R

H

I

I

K

T

A

A

L

F

L

L

G

G

E

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

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M

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S

M

I

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L

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I

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A

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F

F

F

Y

V

G

N

R

P

L

K

K

E

G

M

L

S

S

R

A

V

V

-

V

-

G

-

P

-

E

-

Q

K

D

K

R

L

L

A

L

E

Q

D

D

Y

V

E

G

L

L

E

V

I

S

D

K

P

Q

D

S

A

V

R

Q

I

T

N

R

Q

H

L

L

S

S

M

G

G

G

E

M

K

Q

Q

R

R

K

V

L

E

S

I

V

L

A

K

I

L

A

L

F

Y

V

R

G

E

G

S

S

R

Q

V

V

L

V

I

I

F

L

D

D

E

E

P

P

T

T

A

A

V

G

L

V

T

D

P

P

R

A

E

S

T

R

F

R

R

G

L

I

F

W

E

E

A

L

L

L

W

-

A

L

M

K

T

Y

R

R

Q

E

G

G

K

R

S

T

I

L

V

I

F

L

I

S

S

T

H

H

K

H

L

L

E

D

E

E

V

A

M

E

A

L

I

L

A

G

D

D

E

R

V

V

A

A

I

V

L

V

R

A

R

G

G

G

-

R

-

L

-

C

C

C

V

C

D

G

S

S

E

P

V

L

binding phosphothiophosphoric acid-adenylate ester: F633 (= F34), A640 (= A39), N660 (= N59), G661 (= G60), G663 (= G62), K664 (= K63), T665 (≠ S64), T666 (= T65)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 607, 608, 1507, 1514, 1535, 1536, 1537, 1538, 1539

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
31% identity, 41% coverage: 25:236/513 of query aligns to 3:211/374 of 2awnB

query
sites
2awnB

V

V

S

Q

L

L

K

Q

G

N

I

V

T

T

K

K

R

A

F
x
W

G

G

K

E

V

V

A
x
V

N

S

N

K

K

D

I

I

V

N

L

L

D

D

L

I

Y

H

P

E

G

G

R

E

I

F

K

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

S

R

M

M

L

I

A

A

G

G

R

L

F

E

Q

T

P

I

D

T

E

S

G

G

H

D

I

L

E

F

V

I

D

G

G

E

K

K

R

R

V

M

D

N

F

D

S

T

S

P

K

A

D

E

A

-

I

-

N

-

A

R

G

G

I

V

G

G

M

M

V

V

Y

F

Q

Q

H

S

F

Y

M

A

L

L

V

Y

D

P

S

H

M

L

T

S

V

V

T

A

E

E

N

N

V

M

L

S

L

F

G

G

Q

L

E

K

G

-

F

-

F

L

V

A

N

G

P

A

K

K

E

K

-

E

-

V

M

I

S

N

R

Q

R

R

V

V

K

N

K

Q

L

V

A

A

E

E

D

V

Y

L

E

Q

L

L

E

A

I

H

D

L

P

L

D

D

A

R

R

K

I

P

N

K

Q

A

L

L

S

S

M

G

G

G

E

Q

K

R

Q

Q

R

R

V

V

E

A

I

I

L

G

K

R

L

T

L

L

Y

V

R

A

E

E

S

P

R

S

V

V

L

F

I

L

F

L

D

D

E

E

P

P

T

L

A

S

V

N

L

L

T

D

P

A

-

A

-

L

R

R

E

V

T

Q

F

M

R

R

L

I

F

-

E

E

A

I

L

S

W

R

A

L

M

H

T

K

R

R

Q

L

G

G

K

R

S

T

I

M

V

I

F

Y

I

V

S

T

H

H

K

D

L

Q

E

V

E

E

V

A

M

M

A

T

I

L

A

A

D

D

E

K

V

I

A

V

I

V

L

L

R

D

R

A

G

G

C

R

V

V

binding adenosine-5'-diphosphate: W12 (≠ F34), V17 (≠ A39), S37 (≠ N59), G38 (= G60), G40 (= G62), K41 (= K63), S42 (= S64), T43 (= T65)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
31% identity, 41% coverage: 25:236/513 of query aligns to 4:212/371 of P68187

query
sites
P68187

V

V

S

Q

L

L

K

Q

G

N

I

V

T

T

K

K

R

A

F

W

G

G

K

E

V

V

A

V

N

S

N

K

K

D

I

I

V

N

L

L

D

D

L

I

Y

H

P

E

G

G

R

E

I

F

K

V

A

V

L

F

L

V

G

G

E

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

M

L

S

R

M

M

L

I

A

A

G

G

R

L

F

E

Q

T

P

I

D

T

E

S

G

G

H

D

I

L

E

F

V

I

D

G

G

E

K

K

R

R

V

M

D

N

F

D

S

T

S

P

K

A

D

E

A

-

I

-

N

-

A

R

G

G

I

V

G

G

M

M

V

V

Y

F

Q

Q

H

S

F

Y

M
x
A

L

L

V

Y

D

P

S

H

M

L

T

S

V

V

T

A

E

E

N

N

V

M

L

S

L

F

G

G

Q

L

E

K

G

-

F

-

F

L

V

A

N

G

P

A

K
|
K

E

K

-

E

-

V

M

I

S

N

R

Q

R

R

V
|
V

K

N

K

Q

L
x
V

A

A

E
|
E

D

V

Y

L

E

Q

L

L

E
x
A

I

H

D

L

P

L

D

D

A

R

R

K

I

P

N

K

Q

A

L

L

S

S

M

G

G
|
G

E

Q

K

R

Q

Q

R

R

V

V

E

A

I

I

L

G

K

R

L

T

L

L

Y

V

R

A

E

E

S

P

R

S

V

V

L

F

I

L

F

L

D
|
D

E

E

P

P

T

L

A

S

V

N

L

L

T

D

P

A

-

A

-

L

R

R

E

V

T

Q

F

M

R

R

L

I

F

-

E

E

A

I

L

S

W

R

A

L

M

H

T

K

R

R

Q

L

G

G

K

R

S

T

I

M

V

I

F

Y

I

V

S

T

H

H

K

D

L

Q

E

V

E

E

V

A

M

M

A

T

I

L

A

A

D

D

E

K

V

I

A

V

I

V

L

L

R

D

R

A

G

G

C

R

V

V

A85 (≠ M109) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (= K133) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (= V139) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ L142) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E144) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ E149) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G162) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D183) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
31% identity, 41% coverage: 25:236/513 of query aligns to 1:209/367 of 1q12A

query
sites
1q12A

V

V

S

Q

L

L

K

Q

G

N

I

V

T

T

K

K

R

A

F
x
W

G

G

K

E

V

V

A

V

N

S

N

K

K

D

I

I

V

N

L

L

D

D

L

I

Y

H

P

E

G

G

R

E

I

F

K

V

A

V

L

F

L

V

G

G

E

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

M

L

S

R

M

M

L

I

A

A

G

G

R

L

F

E

Q

T

P

I

D

T

E

S

G

G

H

D

I

L

E

F

V

I

D

G

G

E

K

K

R

R

V

M

D

N

F

D

S

T

S

P

K

A

D

E

A

-

I

-

N

-

A

R

G

G

I

V

G

G

M

M

V

V

Y

F

Q

Q

H

S

F

Y

M

A

L

L

V

Y

D

P

S

H

M

L

T

S

V

V

T

A

E

E

N

N

V

M

L

S

L

F

G

G

Q

L

E

K

G

-

F

-

F

L

V

A

N

G

P

A

K

K

E

K

-

E

-

V

M

I

S

N

R

Q

R

R

V

V

K

N

K

Q

L

V

A

A

E

E

D

V

Y

L

E

Q

L

L

E

A

I

H

D

L

P

L

D

D

A
x
R

R

K

I

P

N

K

Q
x
A

L

L

S
|
S

M

G

G
|
G

E
x
Q

K

R

Q

Q

R

R

V

V

E

A

I

I

L

G

K

R

L

T

L

L

Y

V

R

A

E

E

S

P

R

S

V

V

L

F

I

L

F

L

D

D

E

E

P

P

T

L

A

S

V

N

L

L

T

D

P

A

-

A

-

L

R

R

E

V

T

Q

F

M

R

R

L

I

F

-

E

E

A

I

L

S

W

R

A

L

M

H

T

K

R

R

Q

L

G

G

K

R

S

T

I

M

V

I

F

Y

I

V

S

T

H

H

K

D

L

Q

E

V

E

E

V

A

M

M

A

T

I

L

A

A

D

D

E

K

V

I

A

V

I

V

L

L

R

D

R

A

G

G

C

R

V

V

binding adenosine-5'-triphosphate: W10 (≠ F34), S35 (≠ N59), G36 (= G60), C37 (≠ A61), G38 (= G62), K39 (= K63), S40 (= S64), T41 (= T65), R126 (≠ A154), A130 (≠ Q158), S132 (= S160), G134 (= G162), Q135 (≠ E163)

Query Sequence

>WP_027178119.1 NCBI__GCF_000429985.1:WP_027178119.1
MTDNTKFIRPESGSPAEYASSSPIVSLKGITKRFGKVVANNKIVLDLYPGRIKALLGENG
AGKSTLMSMLAGRFQPDEGHIEVDGKRVDFSSSKDAINAGIGMVYQHFMLVDSMTVTENV
LLGQEGGFFVNPKEMSRRVKKLAEDYELEIDPDARINQLSMGEKQRVEILKLLYRESRVL
IFDEPTAVLTPRETFRLFEALWAMTRQGKSIVFISHKLEEVMAIADEVAILRRGCVDSEV
PREKITSKADLACRMVGKEVLLEIDRNEVEIGDKVLEVKSLNGLGLKNINIEVHRGEVVG
IVGVAGNGQQALVEGVCGLRMPPKDSVFIMGKAWREFFAKMTWNHSLSYIPEDRLDLATA
RELDLVDNLLLTTRQGFCSGPVLHRDKAAEVAKKLVEDHDVRPGRIQALAWQLSGGNLQK
LVLARELYREPHLIVAEQPTQGLDISATEEVWNRLLEARKMAGVLLVTGDLNEALQLSDR
IAVMYRGEIMDQFSVNDTEKVDKVGLMMAGVRE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory