SitesBLAST
Comparing WP_027457611.1 NCBI__GCF_000519045.1:WP_027457611.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
61% identity, 98% coverage: 3:395/399 of query aligns to 4:389/392 of P33221
- EL 22:23 (= EL 21:22) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E81) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R114) binding ATP
- K155 (= K156) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 161:166) binding ATP
- G162 (= G163) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ D180) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFI 196:199) binding ATP
- E203 (= E204) binding ATP
- E267 (= E273) binding Mg(2+)
- E279 (= E285) binding Mg(2+)
- D286 (= D292) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K361) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 368:369) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
61% identity, 98% coverage: 3:395/399 of query aligns to 3:386/389 of 1kjiA
- active site: E114 (= E115), K154 (= K156), S159 (= S161), G161 (= G163), E264 (= E273), E276 (= E285), D283 (= D292), T284 (= T293), R360 (= R369)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R114), I152 (= I154), K154 (= K156), S159 (= S161), S160 (= S162), G161 (= G163), Q164 (= Q166), E192 (= E196), V195 (≠ I199), E200 (= E204), Q222 (= Q231), E264 (= E273), F266 (= F275), E276 (= E285)
- binding magnesium ion: E264 (= E273), E276 (= E285)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
61% identity, 98% coverage: 3:395/399 of query aligns to 3:386/389 of 1ez1A
- active site: E114 (= E115), K154 (= K156), S159 (= S161), G161 (= G163), E264 (= E273), E276 (= E285), D283 (= D292), T284 (= T293), R360 (= R369)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (= I154), K154 (= K156), S159 (= S161), S160 (= S162), G161 (= G163), E192 (= E196), V194 (≠ F198), V195 (≠ I199), F197 (= F201), E200 (= E204), Q222 (= Q231), E264 (= E273), F266 (= F275), E276 (= E285)
- binding glycinamide ribonucleotide: G20 (= G20), E21 (= E21), L22 (= L22), E81 (= E81), I82 (= I82), S160 (= S162), D283 (= D292), K352 (= K361), R359 (= R368), R360 (= R369)
- binding magnesium ion: E264 (= E273), E276 (= E285)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
61% identity, 98% coverage: 3:395/399 of query aligns to 3:386/389 of 1eyzA
- active site: E114 (= E115), K154 (= K156), S159 (= S161), G161 (= G163), E264 (= E273), E276 (= E285), D283 (= D292), T284 (= T293), R360 (= R369)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (= I154), K154 (= K156), S159 (= S161), S160 (= S162), G161 (= G163), Q164 (= Q166), E192 (= E196), V195 (≠ I199), F197 (= F201), E200 (= E204), E264 (= E273), F266 (= F275), E276 (= E285)
- binding magnesium ion: E264 (= E273), E276 (= E285)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
61% identity, 98% coverage: 3:395/399 of query aligns to 3:383/386 of 1kjjA
- active site: E114 (= E115), K154 (= K156), S159 (= S161), G161 (= G163), E261 (= E273), E273 (= E285), D280 (= D292), T281 (= T293), R357 (= R369)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R114), I152 (= I154), K154 (= K156), S159 (= S161), S160 (= S162), G161 (= G163), Q164 (= Q166), E189 (= E196), V192 (≠ I199), E197 (= E204), Q219 (= Q231), E261 (= E273), F263 (= F275), E273 (= E285)
- binding magnesium ion: E261 (= E273), E273 (= E285)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
61% identity, 98% coverage: 3:395/399 of query aligns to 3:383/386 of 1kj8A
- active site: E114 (= E115), K154 (= K156), S159 (= S161), G161 (= G163), E261 (= E273), E273 (= E285), D280 (= D292), T281 (= T293), R357 (= R369)
- binding adenosine-5'-triphosphate: R113 (= R114), I152 (= I154), K154 (= K156), S159 (= S161), S160 (= S162), G161 (= G163), Q164 (= Q166), E189 (= E196), V192 (≠ I199), F194 (= F201), E197 (= E204), Q219 (= Q231), G222 (= G234), E261 (= E273), F263 (= F275), E273 (= E285)
- binding glycinamide ribonucleotide: G20 (= G20), E21 (= E21), L22 (= L22), E81 (= E81), I82 (= I82), S160 (= S162), D280 (= D292), K349 (= K361), R356 (= R368)
- binding magnesium ion: E261 (= E273), E273 (= E285)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
60% identity, 98% coverage: 3:395/399 of query aligns to 3:385/388 of 1kjqA
- active site: E114 (= E115), K154 (= K156), E263 (= E273), E275 (= E285), D282 (= D292), T283 (= T293), R359 (= R369)
- binding adenosine-5'-diphosphate: R113 (= R114), I152 (= I154), K154 (= K156), E191 (= E196), V193 (≠ F198), V194 (≠ I199), F196 (= F201), E199 (= E204), Q221 (= Q231), F265 (= F275), E275 (= E285)
- binding magnesium ion: E263 (= E273), E275 (= E285)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
48% identity, 98% coverage: 2:391/399 of query aligns to 7:405/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
47% identity, 98% coverage: 2:391/399 of query aligns to 9:394/409 of 2dwcB
- active site: E265 (= E273), E277 (= E285), D284 (= D292), T285 (= T293), R372 (= R369)
- binding adenosine-5'-diphosphate: R120 (= R114), H159 (≠ I154), K161 (= K156), H190 (≠ F198), I191 (= I199), F193 (= F201), E196 (= E204), F267 (= F275), E277 (= E285)
4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
30% identity, 81% coverage: 12:335/399 of query aligns to 1:309/366 of 4ma0A
- active site: Y144 (≠ S161), G146 (= G163), E247 (= E273), E259 (= E285), N266 (≠ D292), S267 (≠ T293)
- binding adenosine monophosphate: I136 (= I154), K138 (= K156), E175 (= E196), A176 (≠ G197), F177 (= F198), V178 (≠ I199), E183 (= E204), H206 (= H229), F249 (= F275), E259 (= E285)
Sites not aligning to the query:
5jqwA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with adp
30% identity, 81% coverage: 12:335/399 of query aligns to 1:309/365 of 5jqwA
- active site: Y144 (≠ S161), G146 (= G163), E247 (= E273), E259 (= E285), N266 (≠ D292), S267 (≠ T293)
- binding adenosine-5'-diphosphate: R98 (= R114), K138 (= K156), G143 (≠ S160), Y144 (≠ S161), D145 (≠ S162), G146 (= G163), V178 (≠ I199), E183 (= E204), H206 (= H229), F249 (= F275), E259 (= E285)
Sites not aligning to the query:
4mamA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an adp analog, amp-cp
30% identity, 81% coverage: 12:335/399 of query aligns to 1:309/373 of 4mamA