SitesBLAST
Comparing WP_027458048.1 NCBI__GCF_000519045.1:WP_027458048.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7fj9A Kpacka (pduw) with amppnp complex structure
44% identity, 51% coverage: 166:560/768 of query aligns to 3:390/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
44% identity, 51% coverage: 166:560/768 of query aligns to 3:390/395 of 7fj8A
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
42% identity, 53% coverage: 166:573/768 of query aligns to 2:407/408 of P38502
- N7 (= N171) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (≠ I174) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (≠ Q176) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (≠ H178) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R254) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V256) mutation to A: Decreases affinity for acetate.
- L122 (= L285) mutation to A: Decreases affinity for acetate.
- D148 (= D311) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (= F342) mutation to A: Decreases affinity for acetate.
- N211 (≠ I374) mutation to A: Slightly reduced enzyme activity.
- P232 (= P395) mutation to A: Decreases affinity for acetate.
- R241 (= R404) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (≠ D550) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
1tuuA Acetate kinase crystallized with atpgs (see paper)
42% identity, 52% coverage: 166:563/768 of query aligns to 2:397/399 of 1tuuA
- active site: N7 (= N171), R91 (= R254), H180 (= H343), R241 (= R404), E384 (≠ D550)
- binding adenosine-5'-diphosphate: K14 (≠ H178), G210 (= G373), D283 (= D446), F284 (≠ I447), R285 (= R448), G331 (≠ D494), I332 (≠ V495), N335 (≠ T498)
- binding sulfate ion: R91 (= R254), H180 (= H343), G212 (= G375)
1tuuB Acetate kinase crystallized with atpgs (see paper)
42% identity, 52% coverage: 166:563/768 of query aligns to 2:397/398 of 1tuuB
- active site: N7 (= N171), R91 (= R254), H180 (= H343), R241 (= R404), E384 (≠ D550)
- binding adenosine monophosphate: D283 (= D446), R285 (= R448), G331 (≠ D494), I332 (≠ V495), N335 (≠ T498), S336 (= S499)
- binding trihydrogen thiodiphosphate: H180 (= H343), G212 (= G375), R241 (= R404)
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
40% identity, 51% coverage: 167:559/768 of query aligns to 4:387/394 of 4fwsA
- active site: N8 (= N171), R83 (= R254), H172 (= H343), R233 (= R404), E378 (≠ D550)
- binding cytidine-5'-triphosphate: G202 (= G373), N203 (≠ I374), G204 (= G375), D275 (= D446), L276 (≠ I447), R277 (= R448), G323 (≠ D494), I324 (≠ V495), N327 (≠ T498)
- binding 1,2-ethanediol: V21 (≠ T184), C24 (≠ S187), H115 (= H286), N203 (≠ I374), T232 (≠ S403), R233 (= R404), K262 (≠ S433)
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
40% identity, 51% coverage: 167:559/768 of query aligns to 4:387/394 of 4fwrA
- active site: N8 (= N171), R83 (= R254), H172 (= H343), R233 (= R404), E378 (≠ D550)
- binding cytidine-5'-monophosphate: G202 (= G373), N203 (≠ I374), D275 (= D446), L276 (≠ I447), R277 (= R448), G323 (≠ D494), I324 (≠ V495), N327 (≠ T498)
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
40% identity, 51% coverage: 167:559/768 of query aligns to 4:387/394 of 4fwqA
- active site: N8 (= N171), R83 (= R254), H172 (= H343), R233 (= R404), E378 (≠ D550)
- binding guanosine-5'-triphosphate: H172 (= H343), N203 (≠ I374), G204 (= G375), D275 (= D446), L276 (≠ I447), R277 (= R448), E280 (= E451), G323 (≠ D494), I324 (≠ V495), N327 (≠ T498)
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
40% identity, 51% coverage: 167:559/768 of query aligns to 4:387/394 of 4fwpA
- active site: N8 (= N171), R83 (= R254), H172 (= H343), R233 (= R404), E378 (≠ D550)
- binding 1,2-ethanediol: S11 (≠ I174), H115 (= H286), K262 (≠ S433)
- binding guanosine-5'-diphosphate: N203 (≠ I374), D275 (= D446), L276 (≠ I447), R277 (= R448), E280 (= E451), G323 (≠ D494), I324 (≠ V495), N327 (≠ T498), S328 (= S499)
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
40% identity, 51% coverage: 167:559/768 of query aligns to 4:387/394 of 4fwoA
- active site: N8 (= N171), R83 (= R254), H172 (= H343), R233 (= R404), E378 (≠ D550)
- binding guanosine-5'-monophosphate: G202 (= G373), N203 (≠ I374), D275 (= D446), L276 (≠ I447), R277 (= R448), E280 (= E451), G323 (≠ D494), I324 (≠ V495), N327 (≠ T498)
- binding 1,2-ethanediol: E100 (≠ A271), N104 (≠ D275)
4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
40% identity, 51% coverage: 167:559/768 of query aligns to 4:387/394 of 4fwnA
- active site: N8 (= N171), R83 (= R254), H172 (= H343), R233 (= R404), E378 (≠ D550)
- binding adenosine-5'-tetraphosphate: H172 (= H343), H200 (= H371), N203 (≠ I374), G204 (= G375), D275 (= D446), L276 (≠ I447), R277 (= R448), G323 (≠ D494), I324 (≠ V495), N327 (≠ T498)
4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
40% identity, 51% coverage: 167:559/768 of query aligns to 4:387/394 of 4fwmA