SitesBLAST
Comparing WP_027458116.1 NCBI__GCF_000519045.1:WP_027458116.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human) (see 5 papers)
32% identity, 97% coverage: 17:632/636 of query aligns to 49:720/763 of P40939
- V282 (≠ A230) to D: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852773
- I305 (= I250) to N: in MTPD1; mild phenotype with slowly progressive myopathy and sensorimotor polyneuropathy; dbSNP:rs137852774
- L342 (≠ V282) to P: in LCHAD deficiency; dbSNP:rs137852772
- E510 (= E445) active site, For hydroxyacyl-coenzyme A dehydrogenase activity; to Q: in AFLP and LCHAD deficiency; loss of long-chain-3-hydroxyacyl-CoA dehydrogenase activity; dbSNP:rs137852769
6yswA E. Coli anaerobic trifunctional enzyme subunit-alpha in complex with coenzyme a
34% identity, 99% coverage: 8:636/636 of query aligns to 4:666/707 of 6yswA
- active site: A66 (= A69), I71 (≠ F74), A84 (≠ V87), Q88 (≠ W91), G112 (= G115), E115 (= E118), P136 (= P139), E137 (= E140), G145 (= G148), D264 (≠ L261), S422 (= S412), H443 (= H433), E455 (= E445), N493 (= N483)
- binding coenzyme a: E23 (= E27), M25 (≠ A29), A66 (= A69), D67 (= D70), I68 (= I71), P136 (= P139), E137 (= E140), L140 (= L143), T290 (≠ E287), K293 (= K290)
P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 98% coverage: 10:632/636 of query aligns to 10:674/729 of P21177
- G116 (= G115) mutation to F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected.
- G322 (= G309) mutation to A: 10-fold increase in KM for NADH.
- H450 (= H433) active site, For 3-hydroxyacyl-CoA dehydrogenase activity; mutation H->A,Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity.
6tnmA E. Coli aerobic trifunctional enzyme subunit-alpha (see paper)
32% identity, 98% coverage: 10:632/636 of query aligns to 10:674/719 of 6tnmA
- active site: A68 (= A69), F73 (= F74), G116 (= G115), E119 (= E118), P138 (= P139), E139 (= E140), G147 (= G148), N271 (≠ A260), S429 (= S412), H450 (= H433), E462 (= E445), N500 (= N483)
- binding adenosine-5'-triphosphate: D343 (= D330), I344 (≠ Q331), V400 (≠ I383), V401 (≠ F384), V406 (≠ A389), K584 (= K560)
1wdlA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form ii (native4) (see paper)
34% identity, 98% coverage: 15:636/636 of query aligns to 15:678/715 of 1wdlA
- active site: A69 (= A69), N89 (≠ L86), N93 (≠ G90), G117 (= G115), E120 (= E118), P139 (= P139), E140 (= E140), P147 (= P147), G148 (= G148), S430 (= S412), H451 (= H433), E463 (= E445), N501 (= N483)
- binding nicotinamide-adenine-dinucleotide: A322 (= A308), I324 (≠ V310), M325 (= M311), D344 (= D330), I345 (≠ Q331), A400 (= A382), V401 (≠ I383), E403 (= E385), N428 (= N410), T429 (= T411), S430 (= S412)
P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi (see paper)
34% identity, 98% coverage: 15:636/636 of query aligns to 15:678/715 of P28793
- D297 (≠ Q286) binding substrate
- M325 (= M311) binding NAD(+)
- D344 (= D330) binding NAD(+)
- VVE 401:403 (≠ IFE 383:385) binding NAD(+)
- K408 (= K390) binding NAD(+)
- S430 (= S412) binding NAD(+)
- N454 (= N436) binding NAD(+)
- N501 (= N483) binding substrate
- Y660 (≠ F618) binding substrate
1wdmA Fatty acid beta-oxidation multienzyme complex from pseudomonas fragi, form i (native3) (see paper)
34% identity, 98% coverage: 15:636/636 of query aligns to 15:670/707 of 1wdmA
- active site: A69 (= A69), N89 (≠ L86), N93 (≠ G90), G117 (= G115), E120 (= E118), P139 (= P139), E140 (= E140), P147 (= P147), G148 (= G148), S430 (= S412), H451 (= H433), E463 (= E445), N501 (= N483)
- binding acetyl coenzyme *a: K142 (≠ M142), D297 (≠ Q286), M459 (= M441), N501 (= N483), P534 (= P516), Y652 (≠ F618), L658 (≠ P624)
- binding nicotinamide-adenine-dinucleotide: G321 (= G307), A322 (= A308), I324 (≠ V310), M325 (= M311), D344 (= D330), V401 (≠ I383), E403 (= E385), N428 (= N410), S430 (= S412), N454 (= N436)
7o4uA Structure of the alpha subunit of mycobacterium tuberculosis beta- oxidation trifunctional enzyme in complex with oxidized nicotinamide adenine dinucleotide (see paper)
30% identity, 99% coverage: 4:632/636 of query aligns to 2:673/711 of 7o4uA
8oqsB Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-83
30% identity, 99% coverage: 4:632/636 of query aligns to 19:697/735 of 8oqsB
- binding 4-phenylbenzenesulfonic acid: M45 (≠ L32), I50 (≠ L37), G82 (= G68), G83 (≠ A69), D84 (= D70), T87 (≠ E73), T87 (≠ E73), M88 (≠ F74), M88 (≠ F74), A91 (≠ L77), D95 (≠ E81), D98 (≠ R84), V99 (≠ A85), T102 (vs. gap), T102 (vs. gap), E134 (= E118), E156 (= E140), L159 (= L143), F302 (≠ I271), F302 (≠ I271), F319 (≠ G284), S456 (= S412), T457 (≠ S413), M485 (= M441), P486 (= P442), G523 (= G479), S527 (≠ N483), N535 (≠ L491), P560 (= P516), M575 (vs. gap), I578 (vs. gap), I578 (vs. gap), I682 (= I617), M683 (≠ F618), G686 (= G621)
Sites not aligning to the query:
8oqoA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-49
30% identity, 99% coverage: 4:632/636 of query aligns to 12:690/727 of 8oqoA
8oqlA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-1
30% identity, 99% coverage: 4:632/636 of query aligns to 12:690/728 of 8oqlA
- binding Hexafluorophosphate anion: V37 (≠ S31), N39 (≠ S33), G75 (= G68), D77 (= D70), M81 (≠ F74), V92 (≠ A85), T95 (vs. gap), P148 (= P139), E149 (= E140), L152 (= L143), Q180 (≠ T170), Q260 (= Q233), K362 (≠ Q329), D363 (= D330), V364 (≠ Q331), V430 (≠ L393), D476 (≠ S439), K477 (≠ M440), M478 (= M441), P479 (= P442), K506 (= K469)
- binding formamide: V78 (≠ I71), K79 (≠ S72)
8oqrA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-80
30% identity, 99% coverage: 4:632/636 of query aligns to 13:691/728 of 8oqrA
- binding 4-cyanobenzenesulfonic acid: G76 (= G68), G77 (≠ A69), T81 (≠ E73), M82 (≠ F74), M82 (≠ F74), A85 (≠ L77), D89 (≠ E81), T96 (vs. gap), L123 (= L113), G124 (= G114), P149 (= P139), E150 (= E140), S366 (≠ A332), L367 (= L333), E368 (= E334), A420 (= A382), V421 (≠ I383), F422 (= F384)
8oqvA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-109
30% identity, 99% coverage: 4:632/636 of query aligns to 11:689/726 of 8oqvA
- binding 4-nitrobenzenesulfonic acid: E39 (≠ A34), G74 (= G68), M80 (≠ F74), V91 (≠ A85), T94 (vs. gap), T94 (vs. gap), E148 (= E140), L151 (= L143), R182 (≠ T173), S448 (= S412), S519 (≠ N483), R520 (≠ A484), L562 (≠ V526), L566 (vs. gap), I570 (vs. gap)
Sites not aligning to the query:
4b3iA Crystal structure of mycobacterium tuberculosis fatty acid beta- oxidation complex with coenzymea bound at the hydratase active sites (see paper)
30% identity, 99% coverage: 4:632/636 of query aligns to 15:693/731 of 4b3iA
- active site: G79 (≠ A69), E100 (= E88), R104 (≠ K92), G127 (= G115), E130 (= E118), P151 (= P139), E152 (= E140), G160 (= G148), S452 (= S412), H473 (= H433), E485 (= E445), S523 (≠ N483)
- binding adenosine-5'-diphosphate: Q640 (≠ E587), P641 (≠ R588), P642 (≠ L589), L643 (≠ V590), Q644 (≠ K591)
- binding coenzyme a: T38 (≠ A29), V40 (≠ S31), A77 (= A67), G79 (≠ A69), D80 (= D70), V81 (≠ I71), E152 (= E140), F315 (≠ G284), Q319 (vs. gap)
8oqqA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-79
30% identity, 99% coverage: 4:632/636 of query aligns to 7:685/723 of 8oqqA
8oqpA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-76
30% identity, 99% coverage: 4:632/636 of query aligns to 7:685/723 of 8oqpA
- binding 2-azanyl-5-sulfo-benzoic acid: G28 (≠ E27), S29 (= S28), A63 (≠ G60), K64 (= K61), K64 (= K61), K65 (≠ A62), P143 (= P139), E144 (= E140), L147 (= L143), F307 (≠ G284), M473 (= M441), P548 (= P516), S599 (≠ L557), L602 (≠ K560), K603 (≠ S561), S661 (≠ D608), T662 (≠ A609), G674 (= G621)
Sites not aligning to the query:
8pf8A Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-72
30% identity, 99% coverage: 4:632/636 of query aligns to 13:691/729 of 8pf8A
- binding [2-methyl-5-(trifluoromethyl)pyrazol-3-yl]boronic acid: G76 (= G68), F169 (≠ V159), N173 (≠ A162), S177 (≠ D166), I193 (≠ M182), F313 (≠ G284)
- binding bis[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]-bis(oxidanyl)boranuide: M39 (≠ L32), N40 (≠ S33), E41 (≠ A34), T81 (≠ E73), D92 (≠ R84), V93 (≠ A85)
- binding [(2~{R})-2,3-bis(oxidanyl)propoxy]-[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]borinic acid: M39 (≠ L32), G77 (≠ A69), D78 (= D70), M82 (≠ F74), V93 (≠ A85)
- binding (2~{R})-3-bis[2-methyl-5-(trifluoromethyl)pyrazol-3-yl]boranyloxypropane-1,2-diol: T152 (≠ M142), R184 (≠ T173), A311 (≠ V282), F312 (= F283), I673 (≠ A614)
Sites not aligning to the query:
8oquA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-92
30% identity, 99% coverage: 4:632/636 of query aligns to 14:692/730 of 8oquA
- binding 4-chloranylbenzenesulfonic acid: M40 (≠ L32), N41 (≠ S33), E42 (≠ A34), G77 (= G68), G78 (≠ A69), D79 (= D70), V80 (≠ I71), D90 (≠ E81), V94 (≠ A85), L124 (= L113), G125 (= G114), P150 (= P139), E151 (= E140)
8oqtA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-91
30% identity, 99% coverage: 4:632/636 of query aligns to 13:691/729 of 8oqtA
- binding 4-bromanylbenzenesulfonic acid: E41 (≠ A34), G76 (= G68), G77 (≠ A69), D78 (= D70), V79 (≠ I71), M82 (≠ F74), D89 (≠ E81), V93 (≠ A85), T96 (vs. gap), T96 (vs. gap), P149 (= P139), E150 (= E140)
Sites not aligning to the query:
8oqnA Structure of mycobacterium tuberculosis beta-oxidation trifunctional enzyme in complex with fragment-m-53
30% identity, 99% coverage: 4:632/636 of query aligns to 13:691/729 of 8oqnA
- binding 1-benzyl-1H-pyrazole-4-carboxylic acid: M39 (≠ L32), M82 (≠ F74), E150 (= E140), Q172 (≠ P161), F175 (≠ A164), V176 (≠ L165), Q181 (≠ T170), T241 (vs. gap), F254 (≠ V225), N257 (= N228), Q261 (= Q233), L262 (≠ V234), P266 (= P240), P268 (= P244), Q282 (≠ A260), V283 (≠ L261), G302 (vs. gap), Q303 (≠ P274), V304 (≠ T275), S521 (≠ N483), G525 (≠ A487)
Query Sequence
>WP_027458116.1 NCBI__GCF_000519045.1:WP_027458116.1
MNVNLQHWRLDVDAQGIATATLDKAGESANSLSAEVLREFSSLLDQLDVYPPKGLIIRSG
KAAGFIAGADISEFSQLDTAEKGRALVERGWKLFNRLEAVKYPTLALVRGHCLGGGLELA
LACRYLLAVDESGTKMGLPEVMLGIFPGWGGMLRLPQRVGPAAALDMMLTGKTLDAKRAK
KMGLADDCVPPRVMEMAARQLVVSGQPRRPLPFMQKLMNGPLKSVVANGARKQVAKKARP
QHYPAPYAIIDLWQKHDGNALAAPEVVDRIIASPTARNLVRVFGLQERLKGFGKDSDFKA
RRVHVIGAGVMGGDIAAWCALRGMTVTLQDQALERIAPALKRAQSLFAKKLRDPLKARAA
FDRLIPDPQGDGVPHADVIIEAIFENVEAKHALFRALEPRMKPGAVLATNTSSLKLEDLR
TVLHRPERLVGIHFFNPVSMMPLVEVVEAEGADPAAVQAACAFVKQIDKLPLPVKSAPGF
LVNAVLAPYMLAAMRAVDEGISPETVDEAMLAFGMPMGPIELIDTVGLDIAMAAGKQLAG
GADAPKCLLQRVEKNLLGKKSGQGFYAWSEGKPQKGSAGSVPAGLAERLVKPLIDRTERL
VAEGIIGDAELADAGVIFGTGFAPFTGGPLNYRKSV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory