SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H30) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D34) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ Y81) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H106) mutation to H: Little effect on the kinetic properties.
E349 (≠ D365) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
30% identity, 62% coverage: 2:394/635 of query aligns to 1:366/497 of 1ct9A

query
sites
1ct9A

C
x
A

G

S

I

I

T

F

G

G

I

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F

D

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D

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N
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E

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-

A

G

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D
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M

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-

D

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D

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P

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C

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A

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-

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D

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N

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-

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-

A

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S

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I

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I

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S

A

A

M

I

M

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A

K

G

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N

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x
A

I
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V

G

G

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D

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x
D

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D
x
E

G

G

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D

E

E

S

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F

F

G

G

Y

Y

R

L

R

Y

Y

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H

-

K

-

A

-

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-

N

-

A

L

K

H

E

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L

M

H

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x
M

active site: A1 (≠ C2), L50 (= L51), N74 (= N75), G75 (= G76), T305 (≠ P338), R308 (≠ D341), E332 (≠ D365), M366 (≠ L394)
binding adenosine monophosphate: L232 (≠ F264), L233 (= L265), S234 (= S266), S239 (= S271), A255 (≠ S290), V256 (≠ I291), D263 (≠ E300), M316 (≠ V350), S330 (= S363), G331 (= G364), E332 (≠ D365)
binding glutamine: A1 (≠ C2), R49 (= R50), L50 (= L51), I52 (= I53), V53 (≠ I54), N74 (= N75), G75 (= G76), E76 (= E77), D98 (= D100)

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 59% coverage: 1:374/635 of query aligns to 1:365/557 of P78753

query
sites
P78753

M

M

C

C

G

G

I

I

T

L

G

A

I

V

F

H

D

H

T

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R

A

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E

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D

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I

D

E

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A

D

F

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K

A

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K

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A

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L

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M

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Q

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D

D

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G

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A

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N

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D

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D

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G

N

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C

E

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A

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Y

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G

G

M

M

F

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D

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A

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A

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G

Y

Y

Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
28% identity, 59% coverage: 1:374/635 of query aligns to 1:374/561 of P08243

query
sites
P08243

M

M

C
|
C

G

G

I

I

T

W

G
x
A

I

L

F

F

D

G

T

S

R

D

S

D

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C

R

L

D

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A

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C

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A

M

M

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D

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A

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N

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G

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E

K

D

Y

K

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C

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V

L

V

C

F

Y

N

N

G

G

E

E

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I

Y

Y

N

N

Y

H

Q

K

E

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M

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P

-

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L

Q

Q

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A

H

L

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G

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V

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F

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A

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L

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A

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A

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D

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Y

Y

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Y

T

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S

A

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S

A

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I

V

P

G

T

M

Y

Y

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I

C

S

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K

L

Y

A

I

R

R

K

K

H

N

V

T

-

D

-

S

T

V

V

V

A

I

L
x
F

S

S

G

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G

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G

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Y

Y

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ Q219) to E: in dbSNP:rs1049674
F362 (≠ L362) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
27% identity, 59% coverage: 2:374/635 of query aligns to 1:361/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

I

I

T
x
W

G

A

I

L

F

F

D

G

T

S

R

D

S

D

R

C

R

L

D

S

I

V

D

Q

R

-

A

-

V

C

L

L

H

S

R

A

M

M

N

K

E

I

A

A

Q

-

F

-

H

H

R

R

G

G

P

P

D

D

E

A

G

F

G

R

L

F

H

E

I

N

E

V

P

N

G

G

-

Y

-

T

-

N

L

C

G

C

L

F

G

G

H

F

R

H

R
|
R

L
|
L

S

A

I
x
V

D

D

L

P

S

L

T

F

G

G

Q

M

Q

Q

P

P

L

I

-

R

Y

V

N

K

E

K

D

Y

K

P

S

Y

V

L

C

W

V

L

V

C

F
x
Y

N
|
N

G
|
G

E
|
E

I

I

Y

Y

N

N

Y

H

Q

K

E

K

L

M

I

-

P

-

E

-

L

Q

Q

Q

A

H

L

F

G

E

H

F

V

E

F

Y

H

Q

T

T

R

K

S
x
V

D
|
D

T

G

E

E

V

I

I

I

V

L

H

H

A

L

W

Y

E

D

A

K

W

G

G

G

-

I

E

E

R

Q

C

T

V

I

D

C

R

M

F

L

R

D

G

G

M

V

F

F

A

A

F

F

A

V

L

L

W

L

D

D

R

T

N

A

R

N

E

K

T

K

L

V

F

F

L

L

A

G

R

R

D

D

R

T

L

Y

G

G

V

V

K

R

P

P

L

L

F

F

Y

K

A

A

L

M

L

T

D

E

D

D

G

G

S

F

F

L

L

A

F

V

G

C

S

S

E

E

L

A

K

K

S

G

L

L

L

V

A

T

H

-

G

-

L

L

K

K

R

H

D

S

I

A

D

T

P

P

C

F

A

L

V

K

E

V

E

E

Y

P

F

F

A

L

L

P

G

G

Y

H

V

Y

A

-

E

-

P

-

R

-

A

-

I

-

F

-

K

-

Q

E

A

V

R

L

K

D

L

L

P

K

P

P

A

N

C

G

T

K

L

V

L

A

L

S

R

V

R

E

G

M

Q

V

P

K

V

Y

G

H

Q

H

P

C

R

R

E

D

Y

V

W

F

D

P

V

-

R

G

F

F

T

E

L

I

D

E

N

T

P

-

I

-

S

-

D

-

A

-

D

-

A

V

Q

K

A

N

E

N

L

L

T

R

H

I

R

L

L

F

E

N

S

A

I

V

K

K

L

K

R

R

M

L

I

M

S

T

E

D

V

R

P

R

L

I

G

G

A

C

F

L

L

L

S

S

G

G

V

L

D

D

S

S

A

L

V

V

V

A

A

A

M

T

M

L

-

L

-

K

-

Q

-

L

-

K

A

E

G

A

L

Q

S

V

A

Q

N

Y

P

P

V

L

N

Q

T

T

C

F

S

A

I

I

G

G

F

M

S

E

D

D

P

S

A

P

F

-

N

-

E

D

S

L

E

L

F

A

A

A

R

R

M

K

V

V

A

A

D

D

R

H

Y

I

H

G

T

S

N

E

H

H

H

Y

L

E

D

V

V

L

V

F

E

N

S

S

D

E

-

G

-

I

-

Q

-

A

F

L

D

D

L

E

I

V

D

I

T

F

L

S

A

L

R

E

L

T

Y

Y

D

D

E

I

P
x
T

Y

T

A

V

D
x
R

S

A

S

S

A

-

I

V

P

G

T

M

Y

Y

R

L

V

I

C

S

Q

K

L

Y

A

I

R

R

K

K

H

N

V

T

-

D

-

S

T

V

V

V

A

I

L

F

S

S

G

G

D

E

G

G

G

S

D

D

E

E

S

L

F

T

G

Q

G

G

Y

Y

active site: W4 (≠ T5), L49 (= L51), N74 (= N75), G75 (= G76), T324 (≠ P338), R327 (≠ D341)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R50), V51 (≠ I53), V52 (≠ I54), Y73 (≠ F74), N74 (= N75), G75 (= G76), E76 (= E77), V95 (≠ S99), D96 (= D100)

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
24% identity, 44% coverage: 2:279/635 of query aligns to 87:377/561 of Q9STG9

query
sites
Q9STG9

C

C

G

G

I

V

T

V

G

G

I

I

F

Y

D

G

T

D

R

S

S

E

R

A

R

S

D

R

I

L

D

C

R

Y

A

L

V

A

L

L

H

H

R

A

M

L

N

Q

E

-

A

-

Q

-

F

-

H

H

R

R

G

G

P

Q

D

E

E

G

G

A

G

G

-

I

-

V

-

T

-

V

-

S

-

K

-

D

-

K

-

V

L

L

H

Q

I

T

E

I

P

T

G

G

L

V

G

G

L

L

-

V

-

S

-

E

-

V

-

F

-

S

-

E

-

S

-

K

-

L

-

D

-

Q

-

L

-

P

-

G

-

D

-

I

-

A

-

I

G

G

H

H

R

V

R

R

L

Y

S

S

I

T

I

A

D

G

L

S

S

S

-

M

-

L

T

K

G

N

Q

V

Q

Q

P

P

L

F

Y

V

N

A

E

G

D

Y

K

R

-

F

-

G

S

S

V

V

C

G

V

V

V

A

F
x
H

N

N

G

G

E

N

I

L

Y

V

N

N

Y

Y

Q

T

E

K

L

L

I

R

P

A

E

D

L

L

Q

E

A

E

L

N

G

G

H

S

V

I

F

F

H

N

T

T

R

S

S

S

D

D

T

T

E

E

V

V

I

V

V

L

H

H

A

L

W

I

E

A

A

I

W

S

G

K

E

A

R

R

-

P

-

F

-

M

-

R

-

I

-

V

-

D

C

A

V

C

D

E

R

K

F

L

R

Q

G

G

M

A

F

Y

A

S

F

M

A

V

L

F

W

V

D

T

R

E

N

D

R

K

E

-

T

-

L

L

F

V

L

A

A

V

R

R

D

D

R

P

L

H

G

G

V

F

K
x
R

P
|
P

L

L

F

V

Y

M

A

G

L

R

D

S

D

N

G

G

S

A

F

V

L

V

F

F

G

A

S

S

E

E

L

T

K

C

S

A

L

L

-

D

L

L

A

I

H

E

G

A

G

T

L

Y

K

E

R

R

D

E

I

V

D

Y

P

P

C

-

A

-

V

-

E

-

Y

-

F

-

A

-

L

-

G

-

Y

-

V

-

A

G

E

E

P

V

R

L

A

V

I

V

F

D

K

K

Q

D

A

G

R

V

K

K

L

-

P

-

A

-

C

C

T

Q

L

C

L

L

L

M

R

-

G

-

Q

-

P

P

V

H

G

P

Q

E

P

P

R

K

E

Q

-

C

-

I

Y

F

W

E

D

H

V

I

R

Y

F

F

T

S

L

L

D

P

N

N

P

S

I

I

S

V

D

F

A

G

D

R

A

S

Q

V

A

Y

E

E

L

S

T

R

H

H

R

V

L

F

E

G

E

E

S

I

I

-

K

-

L

-

R

-

M

L

I

A

S

T

E

E

V

S

P

P

L

V

G

D

A

C

F

D

L

V

S

V

G

I

G

A

V

V

D

P

S

D

S

S

A
x
G

V

V

A

A

M

A

M

L

A

G

H187 (≠ F74) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K145) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P146) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.
G371 (≠ A273) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734.

Sites not aligning to the query:

476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
25% identity, 28% coverage: 2:178/635 of query aligns to 1:212/460 of 6lbpA

query
sites
6lbpA

C
|
C

G

G

I

V

T

V

G

G

I

I

F

Y

D

G

T

D

R

S

S

E

R

A

R

S

D

R

I

L

D

C

R

Y

A

L

V

A

L

L

H

H

R

A

M

L

N

Q

E

-

A

-

Q

-

F

-

H

H

R

R

G
|
G

P

Q

D

E

E

G

G

A

G

G

-

I

-

V

-

T

-

V

-

S

-

K

-

D

-

K

-

V

L

L

H

Q

I

T

E

I

P

T

G

G

L

V

G

G

L

L

-

V

-

S

-

E

-

V

-

F

-

S

-

E

-

S

-

K

-

L

-

D

-

Q

-

L

-

P

-

G

-

D

-

I

-

A

-

I

G

G

H

H

R

V

R

R

L

Y

S

S

I

T

I

A

D

G

L

S

S

S

-

M

-

L

T

K

G

N

Q

V

Q

Q

P

P

L

F

Y

V

N

A

E

G

D

Y

K

R

-

F

-

G

S

S

V

V

C

G

V

V

V

A

F

H

N
|
N

G
|
G

E

N

I

L

Y

V

N

N

Y

Y

Q

T

E

K

L

L

I

R

P

A

E

D

L

L

Q

E

A

E

L

N

G

G

H

S

V

I

F

F

H

N

T

T

R

S

S

S

D

D

T

T

E

E

V

V

I

V

V

L

H

H

A

L

W

I

E

A

A

I

W

S

G

K

E

A

R

R

-

P

-

F

-

M

-

R

-

I

-

V

-

D

C

A

V

C

D

E

R

K

F

L

R

Q

G

G

M

A

F

Y

A

S

F

M

A

V

L

F

W

V

D

T

R

E

N

D

R

K

E

-

T

-

L

L

F

V

L

A

A

V

R

R

D

D

R

P

L

H

G

G

V

F

K

R

P

P

L

L

F

V

Y

M

A

G

L

R

D

S

D

N

G

G

S

A

F

V

L

V

F

F

G

A

S

S

E

E

L

T

K

C

S

A

L

L

-

D

L

L

A

I

H

E

G

A

G

T

L

Y

K

E

R

R

D

E

I

V

D

Y

P

P

active site: C1 (= C2), G27 (= G32), N102 (= N75), G103 (= G76)

Sites not aligning to the query:

active site: 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

1q19A Carbapenam synthetase (see paper)
34% identity, 18% coverage: 261:373/635 of query aligns to 240:352/500 of 1q19A

query
sites
1q19A

L

V

G

G

A

I

F
x
P

L
|
L

S
|
S

G

G

V

L

D
|
D

S
|
S

S

S

A

L

V

V

V

T

A

A

M

L

M

-

A

A

G

S

L

R

S

H

A

F

N

K

P

K

V

L

N

N

T

T

C

Y

S
|
S

I
|
I

G

G

F

-

S

-

D

T

P

E

A

L

F

S

N

N

E

E

S

F

E

E

F

F

A

S

R

Q

M

Q

V

V

A

A

D

D

R

A

Y

L

H

G

T

T

N

H

H

H

H

Q

L

M

D

K

V

I

V

L

E

S

S

E

D

T

D

E

F

V

-

I

-

N

D

G

L

I

I

I

D

E

T

S

L

I

A

-

R

-

L

Y

Y

Y

D

N

E

E

P

I

Y

F

A

D

D

G

S
x
L

S

S

A

A

-
x
E

-

I

-

Q

I

S

P

G

T

L

Y

F

R

N

V

V

C

Y

Q

R

L

Q

A

A

R

Q

K

G

H

Q

V

V

T

S

V

C

A

M

L

L

S
x
T

G
|
G

D
x
Y

G
|
G

G

S

D
|
D

E
x
L

S

L

F

F

G

G

active site: L318 (≠ S342), E321 (vs. gap), Y344 (≠ D365)
binding diphosphomethylphosphonic acid adenosyl ester: P243 (≠ F264), L244 (= L265), S245 (= S266), D249 (= D270), S250 (= S271), S268 (= S290), I269 (= I291), T342 (≠ S363), G343 (= G364), D347 (= D368)
binding (2s,5s)-5-carboxymethylproline: Y344 (≠ D365), G345 (= G366), L348 (≠ E369)

Sites not aligning to the query:

active site: 56, 379, 442
binding diphosphomethylphosphonic acid adenosyl ester: 442, 443, 444, 445
binding (2s,5s)-5-carboxymethylproline: 373, 379

Q9XB61 Carbapenam-3-carboxylate synthase; Carbapenam-3-carboxylate ligase; EC 6.3.3.6 from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) (see 3 papers)
34% identity, 18% coverage: 261:373/635 of query aligns to 241:353/503 of Q9XB61

query
sites
Q9XB61

L

V

G

G

A

I

F
x
P

L
|
L

S
|
S

G
|
G

V
x
L

D
|
D

S
|
S

S

S

A

L

V

V

V

T

A

A

M

L

M

-

A

A

G

S

L

R

S

H

A

F

N

K

P

K

V

L

N

N

T

T

C

Y

S

S

I
|
I

G

G

F

-

S

-

D

T

P

E

A

L

F

S

N

N

E

E

S

F

E

E

F

F

A

S

R

Q

M

Q

V

V

A

A

D

D

R

A

Y

L

H

G

T

T

N

H

H

H

H

Q

L

M

D

K

V

I

V

L

E

S

S

E

D

T

D

E

F

V

-

I

-

N

D

G

L

I

I

I

D

E

T

S

L

I

A

-

R

-

L

Y

Y

Y

D

N

E

E

P

I

Y

F

A

D

D

G

S

L

S

S

A

A

-

E

-

I

-

Q

I

S

P

G

T

L

Y

F

R

N

V

V

C

Y

Q

R

L

Q

A

A

R

Q

K

G

H

Q

V

V

T

S

V

C

A

M

L

L

S

T

G
|
G

D
x
Y

G
|
G

G
x
S

D
|
D

E

L

S

L

F

F

G

G

244:251 (vs. 264:271, 75% identical) binding ATP
I270 (= I291) binding ATP
GYGSD 344:348 (≠ GDGGD 364:368) binding ATP
Y345 (≠ D365) mutation to A: Loss of activity.; mutation to F: Reduces catalytic efficiency.
G346 (= G366) binding substrate

Sites not aligning to the query:

371 binding substrate
374 binding substrate
380 E→A: Loss of activity.; E→D: Reduces catalytic efficiency.; E→Q: Reduces catalytic efficiency.
421 binding ATP
443 mutation K->A,M: Loss of activity.
444:446 binding ATP

1ao0A Glutamine phosphoribosylpyrophosphate (prpp) amidotransferase from b. Subtilis complexed with adp and gmp (see paper)
24% identity, 28% coverage: 2:178/635 of query aligns to 1:207/455 of 1ao0A

query
sites
1ao0A

C
|
C

G

G

I

V

T

F

G

G

I

I

F

W

D

G

T

H

R

E

S

E

R

A

R

P

D

Q

I

I

D

T

R

Y

A

Y

V

G

L

L

H

H

R

S

M

L

N

Q

E

-

A

-

Q

-

F

-

H
|
H

R

R

G
|
G

P

Q

D

E

E

G

G

A

G

G

-

I

-

V

-

A

-

T

-

D

-

G

-

E

-

K

L

L

H

T

I

A

E

H

P

K

G

G

L

Q

G

G

L

L

-

I

-

T

-

E

-

V

-

F

-

Q

-

N

-

G

-

E

-

L

-

S

-

K

-

V

-

K

G

G

H

K

R

G

R

A

L

I

S

G

I

H

I

V

D

R

L

Y

S

A

T

T

G

G

Q

Y

-

E

-

N

-

V

Q

Q

P

P

L

L

Y

L

-

F

-

R

-

S

N

Q

E

N

D

N

K

G

S

S

V

L

C

A

V

L

V

A

F

H

N
|
N

G
|
G

E

N

I

L

Y

V

N

N

Y

A

Q

T

E

Q

L

L

I

K

P

Q

E

Q

L

L

Q

E

A

N

L

Q

G

G

H

S

V

I

F

F

H

Q

T

T

R

S

S

S

D

D

T

T

E

E

V

V

I

L

V

A

H

H

A

L

W

I

E

K

A

R

W

S

G

G

-

H

-

F

-

T

-

L

-

K

-

D

-

Q

-

I

E

K

R

N

C

S

V

L

D

S

R

M

F

L

R

K

G

G

M

A

F

Y

A

A

F

F

A

L

L

I

W

M

D

T

R

E

N

T

R

E

E

-

T

-

L

M

F

I

L

V

A

A

R

L

D

D

R

P

L

N

G

G

V

L

K

R

P

P

L

L

F

S

Y

I

A

G

L

M

D

G

D

D

G

-

S

A

F

Y

L

V

F

V

G

A

S

S

E

E

L

T

K

C

S

A

L

F

L

D

A

V

H

V

G

G

G

A

L

T

K

Y

-

L

R

R

D

E

I

V

D

E

P

P

active site: C1 (= C2), G27 (= G32), N98 (= N75), G99 (= G76)
binding adenosine-5'-diphosphate: H25 (= H30)

Sites not aligning to the query:

active site: 238, 296, 301, 311, 419
binding guanosine-5'-monophosphate: 234, 238, 279, 341, 342, 343, 345, 346, 347, 348, 349
binding adenosine-5'-diphosphate: 238, 240, 241, 242, 277, 278, 279, 300, 301, 301, 303
binding magnesium ion: 279, 341, 342
binding iron/sulfur cluster: 232, 233, 234, 378, 380, 433, 436

4amvA E.Coli glucosamine-6p synthase in complex with fructose-6p (see paper)
25% identity, 26% coverage: 2:166/635 of query aligns to 1:197/608 of 4amvA

query
sites
4amvA

C
|
C

G

G

I

I

T

V

G

G

I

A

F

I

D

-

T

-

R

-

S

A

R

Q

R

R

D

D

I

V

D

A

R

E

A

I

V

L

L

L

H

E

R

G

M

L

N

R

E

R

A

L

Q

E

F

Y

H

-

R
|
R

G
|
G

P

Y

D

D

E

S

G

A

G

G

L

L

H

A

I

V

E

V

P

D

G

A

L

E

G

G

L

H

G

M

H

T

R

R

-

L

-

R

-

L

-

G

R

K

L

V

S

Q

I

M

I

L

D

A

L

Q

S

A

T

A

G

E

Q

E

Q

H

P

P

L

L

Y

H

-

G

-

T

-

G

-

I

-

A

-

H

-

T

-

R

-
x
W

-

A

-

T

-

H

-

G

-

E

-

P

-

S

-

E

-

V

-

N

-

A

-

H

-

P

N

H

E

V

D

S

K

E

S

H

V

I

C

V

V

V

F

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

Y

H

Q

E

E

P

L

L

I

R

P

E

E

E

L

L

Q

K

A

A

L

R

G

G

H

Y

V

T

F

F

H

V

T

S

R

E

S

T

D

D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

A

L

W

K

G

Q

E

G

-

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

R

R

C

A

V

I

D

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

D

S

R

G

L

-

G

-

V

-

K

S

P

P

L

L

F

V

Y

I

A

G

L

L

L

G

D

M

D

G

G

E

S

N

F

F

L

I

F

A

G

S

S

D

E

Q

L

L

K

A

S

L

L

L

active site: C1 (= C2), R26 (= R31), G27 (= G32), W74 (vs. gap), N98 (= N75), G99 (= G76)

Sites not aligning to the query:

active site: 248, 481, 485, 488, 504, 603
binding fructose -6-phosphate: 301, 302, 303, 347, 348, 349, 352, 401, 485, 488

1jxaA Glucosamine 6-phosphate synthase with glucose 6-phosphate (see paper)
25% identity, 26% coverage: 2:166/635 of query aligns to 1:197/608 of 1jxaA

query
sites
1jxaA

C
|
C

G

G

I

I

T

V

G

G

I

A

F

I

D

-

T

-

R

-

S

A

R

Q

R

R

D

D

I

V

D

A

R

E

A

I

V

L

L

L

H

E

R

G

M

L

N

R

E

R

A

L

Q

E

F

Y

H

-

R
|
R

G
|
G

P

Y

D

D

E

S

G

A

G

G

L

L

H

A

I

V

E

V

P

D

G

A

L

E

G

G

L

H

G

M

H

T

R

R

-

L

-

R

-

L

-

G

R

K

L

V

S

Q

I

M

I

L

D

A

L

Q

S

A

T

A

G

E

Q

E

Q

H

P

P

L

L

Y

H

-

G

-

T

-

G

-

I

-

A

-

H

-

T

-

R

-
x
W

-

A

-

T

-

H

-

G

-

E

-

P

-

S

-

E

-

V

-

N

-

A

-

H

-

P

N

H

E

V

D

S

K

E

S

H

V

I

C

V

V

V

F

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

Y

H

Q

E

E

P

L

L

I

R

P

E

E

E

L

L

Q

K

A

A

L

R

G

G

H

Y

V

T

F

F

H

V

T

S

R

E

S

T

D

D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

A

L

W

K

G

Q

E

G

-

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

R

R

C

A

V

I

D

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

D

S

R

G

L

-

G

-

V

-

K

S

P

P

L

L

F

V

Y

I

A

G

L

L

L

G

D

M

D

G

G

E

S

N

F

F

L

I

F

A

G

S

S

D

E

Q

L

L

K

A

S

L

L

L

active site: C1 (= C2), R26 (= R31), G27 (= G32), W74 (vs. gap), N98 (= N75), G99 (= G76)

Sites not aligning to the query:

active site: 248, 481, 485, 488, 504, 603
binding glucose-6-phosphate: 302, 303, 347, 348, 349, 352, 401, 485, 488

2j6hA E. Coli glucosamine-6-p synthase in complex with glucose-6p and 5-oxo- l-norleucine (see paper)
25% identity, 26% coverage: 2:166/635 of query aligns to 1:197/608 of 2j6hA

query
sites
2j6hA

C
|
C

G

G

I

I

T

V

G

G

I

A

F

I

D

-

T

-

R

-

S

A

R

Q

R

R

D

D

I

V

D

A

R

E

A

I

V

L

L

L

H

E

R

G

M

L

N

R

E

R

A

L

Q

E

F

Y

H

-

R
|
R

G
|
G

P

Y

D

D

E

S

G

A

G

G

L

L

H

A

I

V

E

V

P

D

G

A

L

E

G

G

L

H

G

M

H

T

R

R

-

L

-

R

-

L

-

G

R

K

L

V

S

Q

I

M

I

L

D

A

L

Q

S

A

T

A

G

E

Q

E

Q

H

P

P

L

L

Y

H

-

G

-

T

-

G

-

I

-

A

-

H

-

T

-
x
R

-
x
W

-

A

-
x
T

-

H

-

G

-

E

-

P

-

S

-

E

-

V

-

N

-

A

-
x
H

-

P

N

H

E

V

D

S

K

E

S

H

V

I

C

V

V

V

F

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

Y

H

Q

E

E

P

L

L

I

R

P

E

E

E

L

L

Q

K

A

A

L

R

G

G

H

Y

V

T

F

F

H

V

T

S

R

E

S

T

D
|
D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

A

L

W

K

G

Q

E

G

-

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

R

R

C

A

V

I

D

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

D

S

R

G

L

-

G

-

V

-

K

S

P

P

L

L

F

V

Y

I

A

G

L

L

L

G

D

M

D

G

G

E

S

N

F

F

L

I

F

A

G

S

S

D

E

Q

L

L

K

A

S

L

L

L

active site: C1 (= C2), R26 (= R31), G27 (= G32), W74 (vs. gap), N98 (= N75), G99 (= G76)
binding 5-oxo-l-norleucine: C1 (= C2), R73 (vs. gap), W74 (vs. gap), T76 (vs. gap), H86 (vs. gap), N98 (= N75), G99 (= G76), D123 (= D100)

Sites not aligning to the query:

active site: 248, 481, 485, 488, 504, 603
binding glucose-6-phosphate: 302, 347, 348, 349, 352, 399, 401, 488

1xfgA Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate (see paper)
25% identity, 26% coverage: 2:166/635 of query aligns to 1:197/238 of 1xfgA

query
sites
1xfgA

C
|
C

G

G

I

I

T

V

G

G

I

A

F

I

D

-

T

-

R

-

S

A

R

Q

R

R

D

D

I

V

D

A

R

E

A

I

V

L

L

L

H

E

R

G

M

L

N

R

E

R

A

L

Q

E

F

Y

H

-

R
|
R

G
|
G

P

Y

D

D

E

S

G

A

G

G

L

L

H

A

I

V

-

V

-

D

-

A

-

E

-

G

-

H

-

M

-

T

-

R

-

L

-

R

-

L

-

G

-

K

-

V

-

Q

-

M

-

L

-

A

-

Q

-

A

-

E

-

H

-

P

-

L

E

H

P

G

G

G

L

T

G

G

L

I

G

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

I

H

D

G

L

E

S

P

T

S

G

E

Q

V

Q

N

P

A

L
x
H

Y

P

N

H

E

V

D

S

K

E

S

H

V

I

C

V

V

V

F

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

Y

H

Q

E

E

P

L

L

I

R

P

E

E

E

L

L

Q

K

A

A

L

R

G

G

H

Y

V

T

F

F

H

V

T

S

R

E

S

T

D
|
D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

A

L

W

K

G

Q

E

G

-

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

R

R

C

A

V

I

D

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

D

S

R

G

L

-

G

-

V

-

K

S

P

P

L

L

F

V

Y

I

A

G

L

L

L

G

D

M

D

G

G

E

S

N

F

F

L

I

F

A

G

S

S

D

E

Q

L

L

K

A

S

L

L

L

active site: C1 (= C2), R26 (= R31), G27 (= G32), W74 (≠ L51), N98 (= N75), G99 (= G76)
binding glutamine hydroxamate: C1 (= C2), R73 (= R50), W74 (≠ L51), T76 (≠ I53), H86 (≠ L63), N98 (= N75), G99 (= G76), D123 (= D100)

1xffA Glutaminase domain of glucosamine 6-phosphate synthase complexed with glutamate (see paper)
25% identity, 26% coverage: 2:166/635 of query aligns to 1:197/238 of 1xffA

query
sites
1xffA

C
|
C

G

G

I

I

T

V

G

G

I

A

F

I

D

-

T

-

R

-

S

A

R

Q

R

R

D

D

I

V

D

A

R

E

A

I

V

L

L

L

H

E

R

G

M

L

N

R

E

R

A

L

Q

E

F

Y

H

-

R
|
R

G
|
G

P

Y

D

D

E

S

G

A

G

G

L

L

H

A

I

V

-

V

-

D

-

A

-

E

-

G

-

H

-

M

-

T

-

R

-

L

-

R

-

L

-

G

-

K

-

V

-

Q

-

M

-

L

-

A

-

Q

-

A

-

E

-

H

-

P

-

L

E

H

P

G

G

G

L

T

G

G

L

I

G

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

I

H

D

G

L

E

S

P

T

S

G

E

Q

V

Q

N

P

A

L
x
H

Y

P

N

H

E

V

D

S

K

E

S

H

V

I

C

V

V

V

F

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

Y

H

Q

E

E

P

L

L

I

R

P

E

E

E

L

L

Q

K

A

A

L

R

G

G

H

Y

V

T

F

F

H

V

T

S

R

E

S

T

D
|
D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

A

L

W

K

G

Q

E

G

-

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

R

R

C

A

V

I

D

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

D

S

R

G

L

-

G

-

V

-

K

S

P

P

L

L

F

V

Y

I

A

G

L

L

L

G

D

M

D

G

G

E

S

N

F

F

L

I

F

A

G

S

S

D

E

Q

L

L

K

A

S

L

L

L

active site: C1 (= C2), R26 (= R31), G27 (= G32), W74 (≠ L51), N98 (= N75), G99 (= G76)
binding glutamic acid: C1 (= C2), R73 (= R50), W74 (≠ L51), T76 (≠ I53), H86 (≠ L63), N98 (= N75), G99 (= G76), D123 (= D100)

Query Sequence

>WP_027458901.1 NCBI__GCF_000519045.1:WP_027458901.1
MCGITGIFDTRSRRDIDRAVLHRMNEAQFHRGPDEGGLHIEPGLGLGHRRLSIIDLSTGQ
QPLYNEDKSVCVVFNGEIYNYQELIPELQALGHVFHTRSDTEVIVHAWEAWGERCVDRFR
GMFAFALWDRNRETLFLARDRLGVKPLFYALLDDGSFLFGSELKSLLAHGGLKRDIDPCA
VEEYFALGYVAEPRAIFKQARKLPPACTLLLRRGQPVGQPREYWDVRFTLDNPISDADAQ
AELTHRLEESIKLRMISEVPLGAFLSGGVDSSAVVAMMAGLSANPVNTCSIGFSDPAFNE
SEFARMVADRYHTNHHLDVVESDDFDLIDTLARLYDEPYADSSAIPTYRVCQLARKHVTV
ALSGDGGDESFGGYRRYKLHLMEEKMRSALPLGLRRPLFGTLGKLYPKADWAPRVFRAKT
TFEGIARSSVEGYFHSVSILRAPMRNQLFSPQFKAALGGYDALQVFTDHAAKADTQDPLA
LIQYLDLKTYLIGDINTKVDRASMAHSLEVREPLMDHKLVEWLATLPSTQKIRGQEGKYL
LKKAMEPHLPNDVLYRPKMGFSVPLARWFRGPLKARVQESILGSRLADTGWFNRDYLQEL
VRDHNNGNRDYSASIWTILMLEAFLRNVMEAGEAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory