SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_027490404.1 NCBI__GCF_021560695.1:WP_027490404.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
45% identity, 97% coverage: 10:313/315 of query aligns to 24:332/339 of Q7XSN8

query
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Q7XSN8

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E219 (= E205) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
D225 (= D211) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.

5cvcA Structure of maize serine racemase (see paper)
45% identity, 97% coverage: 7:313/315 of query aligns to 5:316/329 of 5cvcA

query
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5cvcA

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active site: K52 (= K54), S77 (= S79), E203 (= E205), A207 (= A209), D209 (= D211), G231 (= G233), V306 (≠ L303), S307 (≠ T304)
binding magnesium ion: E203 (= E205), A207 (= A209), D209 (= D211)
binding pyridoxal-5'-phosphate: F51 (= F53), K52 (= K54), N79 (= N81), S178 (≠ G180), G179 (= G181), G180 (= G182), G181 (= G183), L232 (= L234), E275 (= E277), S307 (≠ T304), G308 (= G305)

6zspAAA serine racemase bound to atp and malonate. (see paper)
40% identity, 98% coverage: 6:315/315 of query aligns to 5:315/320 of 6zspAAA

query
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6zspAAA

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active site: K53 (= K54), S74 (= S79), E200 (= E205), A204 (= A209), D206 (= D211), G229 (= G233), L302 (= L303), S303 (≠ T304)
binding adenosine-5'-triphosphate: S28 (= S29), S29 (≠ A30), I30 (≠ A31), K48 (≠ R49), T49 (≠ G50), Q79 (≠ N84), Y111 (≠ A116), E266 (≠ S270), R267 (≠ E271), K269 (= K273), N306 (= N307)
binding magnesium ion: E200 (= E205), A204 (= A209), D206 (= D211)
binding malonate ion: K53 (= K54), S73 (= S78), S74 (= S79), N76 (= N81), H77 (= H82), R125 (= R130), G229 (= G233), S232 (≠ A236)

3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
40% identity, 98% coverage: 6:315/315 of query aligns to 6:318/322 of 3l6bA

query
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3l6bA

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R

Q

F

T

A

V

G

S

-

P

-

E

-

V

R

K

R

N

V

I

G

C

V

I

V

V

L
|
L

T
x
S

G
|
G

G

G

N

N

V

V

D

D

L

L

-

T

D

S

A

S

L

I

P

T

W

W

active site: K54 (= K54), S77 (= S79), E203 (= E205), A207 (= A209), D209 (= D211), G232 (= G233), T278 (≠ S279), L305 (= L303), S306 (≠ T304)
binding malonate ion: K54 (= K54), S76 (= S78), S77 (= S79), N79 (= N81), H80 (= H82), R128 (= R130), G232 (= G233)
binding manganese (ii) ion: E203 (= E205), A207 (= A209), D209 (= D211)
binding pyridoxal-5'-phosphate: F53 (= F53), K54 (= K54), N79 (= N81), G178 (= G180), G179 (= G181), G180 (= G182), G181 (= G183), M182 (≠ L184), V233 (≠ L234), E276 (= E277), T278 (≠ S279), S306 (≠ T304), G307 (= G305)

7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
39% identity, 98% coverage: 6:315/315 of query aligns to 5:322/322 of 7nbgAAA

query
sites
7nbgAAA

G

S

F

F

D

A

D

D

I

V

L

E

A

K

A

A

A

H

A

I

R

N

I

I

A

R

P

D

Y

S

A

I

S

H

V

L

T

T

P

P

V

V

L

L

R

T

S

S

A

S

A

I

L

L

D

N

T

Q

L

L

A

T

G

G

A

R

E

N

L

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

G

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

C

L

N

N

A

A

V

V

W

R

S

S

L

L

D

V

D

P

G

D

-

A

-

L

-

E

Q

R

A

K

A

P

H

K

G

A

V

V

T

T

H

H

S

S

S
|
S

G

G

N
|
N

H

H

G
|
G

N
x
Q

A

A

L

L

A

T

L

Y

A

A

A

K

T

L

R

E

G

G

I

I

A

P

A

A

H

Y

V
x
I

V

V

P

P

E

Q

G

T

A

A

V

P

R

D

A

C

K
|
K

L

K

A

L

A

A

I
|
I

E

Q

R

A

A
x
Y

G

G

A

A

I

S

L

I

H

V

R

Y

C

C

A

E

P

P

T

S

Q

D

A

E

A

S

R

R

E

E

A

N

T

V

C

A

A

K

E

R

V

V

Q

T

R

E

A

E

T

T

G

E

A

G

E

I

L

M

V

V

H

H

P

P

Y

N

A

Q

D

E

T

P

R

A

V

V

M

I

A

A

G

G

Q

Q

G

G

T

T

L

I

A

A

L

L

E

E

L

V

L

L

Q

N

Q

Q

A

V

P

P

G

L

L

V

D

D

A

A

L

L

I

V

T

V

P

P

V

V

G
|
G

L
x
M

A

L

S

A

G

G

V

I

A

A

I

I

A

T

A

V

H

K

G

A

I

L

D

K

P

P

A

S

L

V

D

K

L

V

F

Y

G

A

A

A

E
|
E

P

P

L

S

G

N

A
|
A

D

D

D
|
D

A

C

A

Y

Q

Q

S

S

L

K

A

L

R

K

G

G

T

K

R

L

V

M

T

P

T

N

V

L

V

Y

-

P

P

P

D

E

T

T

V

I

C

A

D

D

G
|
G

L
x
V

R

K

A

S

L

S

V

I

G

G

E

L

R

N

N

T

L

W

D

P

A

I

L

I

R

R

T

D

H

L

R

V

V

D

E

D

V

I

I

F

T

T

V

V

S

T

D

E

A

D

E

E

T

I

V

K

A

C

A

A

M

T

R

Q

L

L

L

V

W

W

S

E

E

R

L

M

K

K

Q

L

V

L

V

I

E

E

V

P

S
x
T

S

A

A

G

T

V

V

G

F

V

A

A

I

V

L

L

K

S

Q

Q

P

H

Q

F

R

Q

F

T

A

V

G

S

-

P

-

E

-

V

R

K

R

N

V

I

G

C

V

I

V

V

L
|
L

T
x
S

G
|
G

G

G

N

N

V

V

D

D

L

L

-

T

D

S

A

S

L

I

P

T

W

W

active site: K53 (= K54), S81 (= S79), E207 (= E205), A211 (= A209), D213 (= D211), G236 (= G233), L309 (= L303), S310 (≠ T304)
binding calcium ion: E207 (= E205), A211 (= A209), D213 (= D211)
binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N81), G182 (= G180), G183 (= G181), G184 (= G182), G185 (= G183), M186 (≠ L184), G236 (= G233), V237 (≠ L234), T282 (≠ S279), S310 (≠ T304), G311 (= G305)
binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (= S79), G85 (= G83), Q86 (≠ N84), I101 (≠ V99), K111 (= K109), I115 (= I113), Y118 (≠ A116)

7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
39% identity, 98% coverage: 6:315/315 of query aligns to 5:322/323 of 7nbfAAA

query
sites
7nbfAAA

G

S

F

F

D

A

D

D

I

V

L

E

A

K

A

A

A

H

A

I

R

N

I

I

A

R

P

D

Y

S

A

I

S
x
H

V
x
L

T
|
T

P
|
P

V

V

L
|
L

R
x
T

S

S

A

S

A

I

L

L

D

N

T

Q

L

L

A

T

G

G

A

R

E

N

L

L

H

F

F

F

K

K

C

C

E

E

N

L

L
x
F

Q

Q

R

K

G

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

C

L

N

N

A

A

V

V

W

R

S

S

L

L

D

V

D

P

G

D

-

A

-

L

-

E

Q

R

A

K

A

P

H

K

G

A

V

V

T

T

H

H

S

S

S
|
S

G

G

N
|
N

H

H

G

G

N

Q

A

A

L

L

A

T

L

Y

A

A

A

K

T

L

R

E

G

G

I

I

A

P

A

A

H

Y

V

I

V

V

P

P

E

Q

G

T

A

A

V

P

R

D

A

C

K

K

L

K

A

L

A

A

I

I

E

Q

R

A

A

Y

G

G

A

A

I

S

L

I

H

V

R

Y

C

C

A

E

P

P

T

S

Q

D

A

E

A

S

R

R

E

E

A

N

T

V

C

A

A

K

E

R

V

V

Q

T

R

E

A

E

T

T

G

E

A

G

E

I

L

M

V

V

H

H

P

P

Y

N

A

Q

D

E

T

P

R

A

V

V

M

I

A

A

G

G

Q

Q

G

G

T

T

L

I

A

A

L

L

E

E

L

V

L

L

Q

N

Q

Q

A

V

P

P

G

L

L

V

D

D

A

A

L

L

I

V

T

V

P

P

V

V

G
|
G

L
x
M

A

L

S

A

G

G

V

I

A

A

I

I

A

T

A

V

H

K

G

A

I

L

D

K

P

P

A

S

L

V

D

K

L

V

F

Y

G

A

A

A

E
|
E

P

P

L

S

G

N

A
|
A

D

D

D
|
D

A

C

A

Y

Q

Q

S

S

L

K

A

L

R

K

G

G

T

K

R

L

V

M

T

P

T

N

V

L

V

Y

-

P

P

P

D

E

T

T

V

I

C

A

D

D

G
|
G

L
x
V

R

K

A

S

L

S

V

I

G

G

E

L

R
x
N

N

T

L

W

D

P

A

I

L

I

R

R

T

D

H

L

R

V

V

D

E

D

V

I

I

F

T

T

V

V

S

T

D

E

A

D

E

E

T

I

V

K

A

C

A

A

M

T

R

Q

L

L

L

V

W

W

S

E

E

R

L

M

K

K

Q

L

V

L

V

I

E

E

V

P

S
x
T

S

A

A

G

T

V

V

G

F

V

A

A

I

V

L

L

K

S

Q

Q

P

H

Q

F

R

Q

F

T

A

V

G

S

-

P

-

E

-

V

R

K

R

N

V

I

G

C

V

I

V

V

L
|
L

T
x
S

G
|
G

G

G

N

N

V

V

D

D

L

L

-

T

D

S

A

S

L

I

P

T

W

W

active site: K53 (= K54), S81 (= S79), E207 (= E205), A211 (= A209), D213 (= D211), G236 (= G233), L309 (= L303), S310 (≠ T304)
binding calcium ion: E207 (= E205), A211 (= A209), D213 (= D211)
binding magnesium ion: N244 (≠ R241)
binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N81), G182 (= G180), G183 (= G181), G184 (= G182), G185 (= G183), M186 (≠ L184), G236 (= G233), V237 (≠ L234), T282 (≠ S279), S310 (≠ T304), G311 (= G305)
binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (≠ S22), L22 (≠ V23), T23 (= T24), P24 (= P25), L26 (= L27), T27 (≠ R28), F46 (≠ L47)

Sites not aligning to the query:

binding magnesium ion: 3

7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
39% identity, 98% coverage: 6:315/315 of query aligns to 5:322/323 of 7nbdAAA

query
sites
7nbdAAA

G

S

F

F

D

A

D

D

I

V

L

E

A

K

A

A

A

H

A

I

R

N

I

I

A

R

P

D

Y

S

A

I

S

H

V

L

T

T

P

P

V

V

L

L

R

T

S

S

A

S

A

I

L

L

D

N

T

Q

L

L

A

T

G

G

A

R

E

N

L

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

G

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

C

L

N

N

A

A

V

V

W

R

S

S

L

L

D

V

D

P

G

D

-

A

-

L

-

E

Q

R

A

K

A

P

H

K

G

A

V

V

T

T

H

H

S

S

S
|
S

G

G

N
|
N

H

H

G

G

N

Q

A

A

L

L

A

T

L

Y

A

A

A

K

T

L

R

E

G

G

I

I

A

P

A

A

H

Y

V

I

V

V

P

P

E

Q

G

T

A

A

V

P

R

D

A

C

K

K

L

K

A

L

A

A

I

I

E

Q

R

A

A

Y

G

G

A

A

I

S

L

I

H

V

R

Y

C

C

A

E

P

P

T

S

Q

D

A

E

A

S

R

R

E

E

A

N

T

V

C

A

A

K

E

R

V

V

Q

T

R

E

A

E

T

T

G

E

A

G

E

I

L

M

V

V

H

H

P

P

Y

N

A

Q

D

E

T

P

R

A

V

V

M

I

A

A

G

G

Q

Q

G

G

T

T

L

I

A

A

L

L

E

E

L

V

L

L

Q

N

Q

Q

A

V

P

P

G

L

L

V

D

D

A

A

L

L

I

V

T

V

P

P

V

V

G
|
G

L
x
M

A

L

S

A

G

G

V

I

A

A

I

I

A

T

A

V

H

K

G

A

I

L

D

K

P

P

A

S

L

V

D

K

L

V

F

Y

G

A

A

A

E
|
E

P

P

L

S

G

N

A
|
A

D

D

D
|
D

A

C

A

Y

Q

Q

S

S

L

K

A

L

R

K

G

G

T

K

R

L

V

M

T

P

T

N

V

L

V

Y

-

P

P

P

D

E

T

T

V

I

C

A

D

D

G
|
G

L
x
V

R

K

A

S

L

S

V

I

G

G

E

L

R
x
N

N

T

L

W

D

P

A

I

L

I

R

R

T

D

H

L

R

V

V

D

E

D

V

I

I

F

T

T

V

V

S

T

D

E

A

D

E

E

T

I

V

K

A

C

A

A

M

T

R

Q

L

L

L

V

W
|
W

S

E

E

R

L

M

K

K

Q

L

V
x
L

V

I

E
|
E

V

P

S
x
T

S

A

A

G

T

V

V

G

F

V

A

A

I

V

L

L

K

S

Q

Q

P

H

Q

F

R

Q

F

T

A

V

G

S

-

P

-

E

-

V

R

K

R

N

V

I

G

C

V

I

V

V

L
|
L

T
x
S

G
|
G

G

G

N

N

V
|
V

D

D

L
|
L

-

T

D

S

A

S

L

I

P

T

W

W

active site: K53 (= K54), S81 (= S79), E207 (= E205), A211 (= A209), D213 (= D211), G236 (= G233), L309 (= L303), S310 (≠ T304)
binding calcium ion: E207 (= E205), A211 (= A209), D213 (= D211)
binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (= W269), L278 (≠ V275), V314 (= V308), L316 (= L310)
binding magnesium ion: N244 (≠ R241)
binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N81), G182 (= G180), G183 (= G181), G184 (= G182), G185 (= G183), M186 (≠ L184), G236 (= G233), V237 (≠ L234), E280 (= E277), T282 (≠ S279), S310 (≠ T304), G311 (= G305)

Sites not aligning to the query:

binding magnesium ion: 3

7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
39% identity, 98% coverage: 6:315/315 of query aligns to 5:322/323 of 7nbcCCC

query
sites
7nbcCCC

G

S

F

F

D

A

D

D

I

V

L

E

A

K

A

A

A

H

A

I

R

N

I

I

A

R

P

D

Y

S

A

I

S

H

V

L

T

T

P

P

V

V

L

L

R

T

S

S

A

S

A

I

L

L

D

N

T

Q

L

L

A

T

G

G

A

R

E

N

L

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

G

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

C

L

N

N

A

A

V

V

W

R

S

S

L

L

D

V

D

P

G

D

-

A

-

L

-

E

Q

R

A

K

A

P

H

K

G

A

V

V

T
|
T

H
|
H

S

S

S
|
S

G

G

N
|
N

H
|
H

G

G

N

Q

A

A

L

L

A

T

L

Y

A

A

A

K

T

L

R

E

G

G

I

I

A

P

A

A

H

Y

V

I

V

V

P

P

E

Q

G

T

A

A

V

P

R

D

A

C

K

K

L

K

A

L

A

A

I

I

E

Q

R

A

A

Y

G

G

A

A

I

S

L

I

H

V

R

Y

C

C

A

E

P

P

T

S

Q

D

A

E

A

S

R

R

E

E

A

N

T

V

C

A

A

K

E

R

V

V

Q

T

R

E

A

E

T

T

G

E

A

G

E

I

L

M

V
|
V

H
|
H

P
|
P

Y

N

A

Q

D

E

T

P

R

A

V

V

M

I

A

A

G

G

Q

Q

G

G

T

T

L

I

A

A

L

L

E

E

L

V

L

L

Q

N

Q

Q

A

V

P

P

G

L

L

V

D

D

A

A

L

L

I

V

T

V

P

P

V

V

G
|
G

L
x
M

A

L

S

A

G

G

V

I

A

A

I

I

A

T

A

V

H

K

G

A

I

L

D

K

P

P

A

S

L

V

D

K

L

V

F

Y

G

A

A

A

E
|
E

P

P

L

S

G

N

A
|
A

D

D

D
|
D

A

C

A

Y

Q

Q

S

S

L

K

A

L

R

K

G

G

T

K

R

L

V

M

T

P

T

N

V

L

V

Y

-

P

P

P

D

E

T

T

V

I

C

A

D

D

G
|
G

L
x
V

R

K

A

S

L

S

V

I

G

G

E

L

R

N

N

T

L

W

D

P

A

I

L

I

R

R

T

D

H

L

R

V

V

D

E

D

V

I

I

F

T

T

V

V

S

T

D

E

A

D

E

E

T

I

V

K

A

C

A

A

M

T

R

Q

L

L

L

V

W

W

S

E

E

R

L

M

K

K

Q

L

V

L

V

I

E

E

V

P

S
x
T

S

A

A

G

T

V

V

G

F

V

A

A

I

V

L

L

K

S

Q

Q

P

H

Q

F

R

Q

F

T

A

V

G

S

-

P

-

E

-

V

R

K

R

N

V

I

G

C

V

I

V

V

L
|
L

T
x
S

G
|
G

G

G

N

N

V

V

D

D

L

L

-

T

D

S

A

S

L

I

P

T

W

W

active site: K53 (= K54), S81 (= S79), E207 (= E205), A211 (= A209), D213 (= D211), G236 (= G233), L309 (= L303), S310 (≠ T304)
binding biphenyl-4-ylacetic acid: T78 (= T76), H79 (= H77), H84 (= H82), V148 (= V146), H149 (= H147), P150 (= P148)
binding calcium ion: E207 (= E205), A211 (= A209), D213 (= D211)
binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N81), G182 (= G180), G183 (= G181), G184 (= G182), G185 (= G183), M186 (≠ L184), G236 (= G233), V237 (≠ L234), T282 (≠ S279), S310 (≠ T304), G311 (= G305)

7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
39% identity, 98% coverage: 6:315/315 of query aligns to 5:322/323 of 7nbcAAA

query
sites
7nbcAAA

G

S

F

F

D

A

D

D

I

V

L

E

A

K

A

A

A

H

A

I

R

N

I

I

A

R

P

D

Y

S

A

I

S

H

V

L

T

T

P

P

V

V

L

L

R

T

S

S

A

S

A

I

L

L

D

N

T

Q

L

L

A

T

G

G

A

R

E

N

L

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

G

T

G

G

A

S

F
|
F

K
|
K

F

I

R

R

G

G

A

A

C

L

N

N

A

A

V

V

W

R

S

S

L

L

D

V

D

P

G

D

-

A

-

L

-

E

Q

R

A

K

A

P

H

K

G

A

V

V

T

T

H

H

S

S

S
|
S

G

G

N
|
N

H

H

G

G

N

Q

A

A

L

L

A

T

L

Y

A

A

A

K

T

L

R

E

G

G

I

I

A

P

A

A

H

Y

V

I

V

V

P

P

E

Q

G

T

A

A

V

P

R

D

A

C

K

K

L

K

A

L

A

A

I

I

E

Q

R

A

A

Y

G

G

A

A

I

S

L

I

H

V

R

Y

C

C

A

E

P

P

T

S

Q

D

A

E

A

S

R

R

E

E

A

N

T

V

C

A

A

K

E

R

V

V

Q

T

R

E

A

E

T

T

G

E

A

G

E

I

L

M

V

V

H

H

P

P

Y

N

A

Q

D

E

T

P

R

A

V

V

M

I

A

A

G

G

Q

Q

G

G

T

T

L

I

A

A

L

L

E

E

L

V

L

L

Q

N

Q

Q

A

V

P

P

G

L

L

V

D

D

A

A

L

L

I

V

T

V

P

P

V

V

G
|
G

L
x
M

A

L

S

A

G

G

V

I

A

A

I

I

A

T

A

V

H

K

G

A

I

L

D

K

P

P

A

S

L

V

D

K

L

V

F

Y

G

A

A

A

E
|
E

P

P

L

S

G

N

A
|
A

D

D

D
|
D

A

C

A

Y

Q

Q

S

S

L

K

A

L

R

K

G

G

T

K

R

L

V

M

T

P

T

N

V

L

V

Y

-

P

P

P

D

E

T

T

V

I

C

A

D

D

G
|
G

L
x
V

R

K

A

S

L

S

V

I

G

G

E

L

R
x
N

N

T

L

W

D

P

A

I

L

I

R

R

T

D

H

L

R

V

V

D

E

D

V

I

I

F

T

T

V

V

S

T

D

E

A

D

E

E

T

I

V

K

A

C

A

A

M

T

R

Q

L

L

L

V

W

W

S

E

E

R

L

M

K

K

Q

L

V

L

V

I

E

E

V

P

S
x
T

S

A

A

G

T

V

V

G

F

V

A

A

I

V

L

L

K

S

Q

Q

P

H

Q

F

R

Q

F

T

A

V

G

S

-

P

-

E

-

V

R

K

R

N

V

I

G

C

V

I

V

V

L
|
L

T
x
S

G
|
G

G

G

N

N

V

V

D

D

L

L

-

T

D

S

A

S

L

I

P

T

W

W

active site: K53 (= K54), S81 (= S79), E207 (= E205), A211 (= A209), D213 (= D211), G236 (= G233), L309 (= L303), S310 (≠ T304)
binding calcium ion: E207 (= E205), A211 (= A209), D213 (= D211)
binding magnesium ion: N244 (≠ R241)
binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N83 (= N81), G182 (= G180), G183 (= G181), G184 (= G182), G185 (= G183), M186 (≠ L184), G236 (= G233), V237 (≠ L234), T282 (≠ S279), S310 (≠ T304), G311 (= G305)

Sites not aligning to the query:

binding magnesium ion: 3

Q9GZT4 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Homo sapiens (Human) (see 4 papers)
39% identity, 98% coverage: 6:315/315 of query aligns to 8:325/340 of Q9GZT4

query
sites
Q9GZT4

G

S

F

F

D

A

D

D

I

V

L
x
E

A

K

A

A

A

H

A

I

R

N

I

I

A

R

P

D

Y

S

A

I

S

H

V

L

T

T

P

P

V

V

L

L

R

T

S
|
S

A
x
S

A
x
I

L

L

D

N

T

Q

L

L

A

T

G

G

A

R

E

N

L

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R
x
K

G
x
T

G

G

A

S

F

F

K
|
K

F

I

R

R

G

G

A

A

C

L

N

N

A

A

V

V

W

R

S

S

L

L

D

V

D
x
P

G

D

-

A

-

L

-

E

Q

R

A

K

A

P

H

K

G

A

V

V

T
|
T

H

H

S

S

G

G

N
|
N

H

H

G

G

N
x
Q

A

A

L

L

A

T

L

Y

A

A

A

K

T

L

R

E

G

G

I

I

A

P

A

A

H

Y

V

I

V

V

P

P

E

Q

G

T

A

A

V

P

R

D

A

C

K

K

L

K

A

L

A

A

I

I

E

Q

R

A

A
x
Y

G

G

A

A

I

S

L

I

H

V

R

Y

C

C

A

E

P

P

T

S

Q

D

A

E

A

S

R

R

E

E

A

N

T

V

C

A

A

K

E

R

V

V

Q

T

R

E

A

E

T

T

G

E

A

G

E

I

L

M

V

V

H

H

P

P

Y

N

A

Q

D

E

T

P

R

A

V

V

M

I

A

A

G

G

Q

Q

G

G

T

T

L

I

A

A

L

L

E

E

L

V

L

L

Q

N

Q

Q

A

V

P

P

G

L

L

V

D
|
D

A

A

L

L

I

V

T

V

P

P

V

V

G
|
G

L
x
M

A

L

S

A

G

G

V

I

A

A

I

I

A

T

A

V

H

K

G

A

I

L

D

K

P

P

A

S

L

V

D

K

L

V

F

Y

G

A

A

A

E
|
E

P

P

L

S

G

N

A
|
A

D

D

D
|
D

A

C

A

Y

Q

Q

S

S

L

K

A

L

R

K

G

G

T

K

R

L

V

M

T

P

T

N

V

L

V

Y

-

P

P

P

D

E

T

T

V

I

C

A

D

D

G

G

L

V

R

K

A

S

L

S

V

I

G

G

E

L

R
x
N

N

T

L

W

D

P

A

I

L

I

R

R

T

D

H

L

R

V

V

D

E

D

V

I

I

F

T

T

V

V

S

T

D

E

A

D

E

E

T

I

V

K

A

C

A

A

M

T

R

Q

L

L

L

V

W

W

S

E

E

R

L

M

K
|
K

Q

L

V

L

V

I

E

E

V

P

S

T

S

A

A

G

T

V

V

G

F

V

A

A

I

V

L

L

K

S

Q

Q

P

H

Q

F

R

Q

F

T

A

V

G

S

-

P

-

E

-

V

R

K

R

N

V

I

G

C

V

I

V

V

L

L

T
x
S

G

G

N
|
N

V

V

D

D

L

L

-

T

D

S

A

S

L

I

P

T

W

W

E13 (≠ L11) binding Mg(2+)
S31 (= S29) binding ATP
S32 (≠ A30) binding ATP
I33 (≠ A31) binding ATP
K51 (≠ R49) binding ATP
T52 (≠ G50) binding ATP
K56 (= K54) modified: N6-(pyridoxal phosphate)lysine
P69 (≠ D67) binding Ca(2+)
T81 (= T76) binding Ca(2+)
N86 (= N81) binding pyridoxal 5'-phosphate
Q89 (≠ N84) binding ATP
Y121 (≠ A116) binding ATP
D178 (= D173) binding Mg(2+)
G185 (= G180) binding pyridoxal 5'-phosphate
G186 (= G181) binding pyridoxal 5'-phosphate
G187 (= G182) binding pyridoxal 5'-phosphate
G188 (= G183) binding pyridoxal 5'-phosphate
M189 (≠ L184) binding pyridoxal 5'-phosphate
E210 (= E205) binding Ca(2+); binding Mg(2+); binding Mn(2+)
A214 (= A209) binding Ca(2+); binding Mg(2+); binding Mn(2+)
D216 (= D211) binding Ca(2+); binding Mg(2+); binding Mn(2+)
N247 (≠ R241) binding Ca(2+); binding Mg(2+)
K279 (= K273) binding ATP
S313 (≠ T304) binding pyridoxal 5'-phosphate
N316 (= N307) binding ATP

7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
40% identity, 97% coverage: 6:310/315 of query aligns to 5:316/320 of 7nbhAAA

query
sites
7nbhAAA

G

S

F

F

D

A

D

D

I

V

L

E

A

K

A

A

A

H

A

I

R

N

I

I

A

R

P

D

Y

S

A

I

S

H

V

L

T

T

P

P

V

V

L

L

R

T

S

S

A

S

A

I

L

L

D

N

T

Q

L

L

A

T

G

G

A

R

E

N

L

L

H

F

F

F

K

K

C

C

E

E

N

L

L

F

Q

Q

R

K

G

T

G

G

A

S

F

F

K
|
K

F

I

R

R

G

G

A

A

C

L

N

N

A

A

V

V

W

R

S

S

L

L

D

V

D

P

G

D

-

A

-

L

-

E

Q

R

A

K

A

P

H

K

G

A

V

V

T

T

H

H

S

S

S
|
S

G

G

N

N

H

H

G
|
G

N
x
Q

A

A

L

L

A

T

L

Y

A

A

A

K

T

L

R

E

G

G

I

I

A

P

A

A

H

Y

V

I

V

V

P

P

E

Q

G

T

A

A

V

P

R

D

A

C

K
|
K

L

K

A

L

A

A

I
|
I

E

Q

R

A

A
x
Y

G

G

A

A

I

S

L

I

H

V

R

Y

C

C

A

E

P

P

T

S

Q

D

A

E

A

S

R

R

E

E

A

N

T

V

C

A

A

K

E

R

V

V

Q

T

R

E

A

E

T

T

G

E

A

G

E

I

L

M

V

V

H

H

P

P

Y

N

A

Q

D

E

T

P

R

A

V

V

M

I

A

A

G

G

Q

Q

G

G

T

T

L

I

A

A

L

L

E

E

L

V

L

L

Q

N

Q

Q

A

V

P

P

G

L

L

V

D

D

A

A

L

L

I

V

T

V

P

P

V

V

G

G

L

M

A

L

S

A

G

G

V

I

A

A

I

I

A

T

A

V

H

K

G

A

I

L

D

K

P

P

A

S

L

V

D

K

L

V

F

Y

G

A

A

A

E
|
E

P

P

L

S

G

N

A
|
A

D

D

D
|
D

A

C

A

Y

Q

Q

S

S

L

K

A

L

R

K

G

G

T

K

R

L

V

M

T

P

T

N

V

L

V

Y

-

P

P

P

D

E

T

T

V

I

C

A

D
|
D

G
|
G

L

V

R

K

A

S

L

S

V

I

G

G

E

L

R

N

N

T

L

W

D

P

A

I

L

I

R

R

T

D

H

L

R

V

V

D

E

D

V

I

I

F

T

T

V

V

S

T

D

E

A

D

E

E

T

I

V

K

A

C

A

A

M

T

R

Q

L

L

L

V

W

W

S

E

E

R

L

M

K

K

Q

L

V

L

V

I

E

E

V
x
P

S

T

S

A

A

G

T

V

V

G

F

V

A

A

I

V

L

L

K

S

Q

Q

P

H

Q

F

R

Q

F

T

A

V

G

S

-

P

-

E

-

V

R

K

R

N

V

I

G

C

V

I

V

V

L
|
L

T
x
S

G

G

N
|
N

V
|
V

D
|
D

L

L

active site: K53 (= K54), S81 (= S79), E207 (= E205), A211 (= A209), D213 (= D211), G236 (= G233), L309 (= L303), S310 (≠ T304)
binding calcium ion: E207 (= E205), A211 (= A209), D213 (= D211)
binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (= S79), G85 (= G83), Q86 (≠ N84), K111 (= K109), I115 (= I113), Y118 (≠ A116), D235 (= D232), P281 (≠ V278), N313 (= N307), V314 (= V308), D315 (= D309)

7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
40% identity, 96% coverage: 7:309/315 of query aligns to 6:310/310 of 7nbgDDD

query
sites
7nbgDDD

F

F

D

A

D

D

I

V

L

E

A

K

A

A

A

H

A

I

R

N

I

I

A

R

P

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D

active site: K53 (= K54), S76 (= S79), E202 (= E205), A206 (= A209), D208 (= D211), G231 (= G233), L304 (= L303), S305 (≠ T304)
binding calcium ion: E202 (= E205), A206 (= A209), D208 (= D211)
binding magnesium ion: N239 (≠ R241)
binding ortho-xylene: S76 (= S79), Q81 (≠ N84), I96 (≠ V99), Y113 (≠ A116)
binding pyridoxal-5'-phosphate: F52 (= F53), K53 (= K54), N78 (= N81), G177 (= G180), G178 (= G181), G179 (= G182), G180 (= G183), M181 (≠ L184), G231 (= G233), V232 (≠ L234), E275 (= E277), T277 (≠ S279), S305 (≠ T304), G306 (= G305)

Sites not aligning to the query:

binding magnesium ion: 3

Q9QZX7 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Mus musculus (Mouse) (see paper)
40% identity, 98% coverage: 6:315/315 of query aligns to 8:324/339 of Q9QZX7

query
sites
Q9QZX7

G

S

F

F

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A

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