SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_027490420.1 NCBI__GCF_021560695.1:WP_027490420.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
58% identity, 90% coverage: 5:304/333 of query aligns to 4:304/304 of 1iyeA

query
sites
1iyeA

F

Y

L

I

W

W

H

F

N

N

G

G

R

E

I

M

K

V

P

R

W

W

A

E

E

D

A

A

S

K

V

V

H

H

V

V

S

M

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

C

Y

Y

A

D

T

S

P

H

R

K

G

G

P

P

A

V

Y

V

F

F

R

R

L

H

A

R

D

E

H

H

T

M

R

Q

R
|
R

L

L

F

H

E

D

S

S

A

A

R

K

V

I

Y

Y

E

R

I

F

E

P

V

V

G

S

Y

Q

S

S

E

I

D

D

E

E

I

L

N

M

A

E

A

A

C

C

L

R

E

D

L

V

I

I

R

R

A

K

N

N

A

N

M

L

P

T

S

S

A

A

Y
|
Y

V

I

R

R

P

P

I

L

V

I

F

F

R

V

G

G

A

D

G

V

G

G

L

M

G

G

V

V

L

N

A

P

K

P

D

A

G

G

A

Y

P

S

V

T

E

D

V

V

A

I

I

I

M

A

A

A

M

F

A

P

W

W

G

G

A

A

Y
|
Y

L

L

G

G

-

A

E

E

A

A

A

L

E

E

R

Q

G

G

A

I

D

D

I

A

C

M

V

V

S

S

S

S

W

W

H

N

R

R

P

A

A

A

P

P

N

N

T

T

I

I

P

P

S

T

W

A

A

A

K
|
K

A
|
A

G

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

Q

L

L

L

I

V

G

G

L

S

E

E

A

A

R

R

R

R

N

H

G

G

Y

Y

D

Q

E

E

G

G

I

I

A

A

L

L

G

D

H

V

G

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N
|
N

L

L

F

F

L

E

V

V

K

K

N

D

G

G

R

V

L

L

L

F

T

T

P

P

P

P

S

F

S

T

A

S

G

S

I

A

L
|
L

A

P

G
|
G

I
|
I

T
|
T

R

R

D

D

S

A

V

I

L

I

A

K

L

L

A

A

A

K

G

E

L

L

G

G

I

I

A

E

V

V

E

R

E

E

R

Q

E

V

L

L

P

S

R

R

E

E

A

S

L

L

Y

Y

S

L

A

A

D

D

E

E

V

V

F

F

M

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

R

G

K

I

A

Q

V

V

G

G

K

E

G

G

A

R

P

C

G

G

P

P

V

V

T

T

Q

K

Q

R

L

I

R

Q

Q

Q

A

A

F

F

F

F

G

G

L

L

F

F

D

T

G

G

R

E

T

T

E

E

D

D

R

K

H

W

G

G

W

W

L

L

S

D

V

Q

V

V

N

N

active site: F33 (= F34), G35 (= G36), K156 (= K156), A157 (= A157), E190 (= E190), L214 (= L214)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R57), Y92 (= Y93), Y126 (= Y127), K156 (= K156), Y161 (= Y161), E190 (= E190), G193 (= G193), E194 (= E194), N195 (= N195), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254), A255 (= A255)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
58% identity, 90% coverage: 5:304/333 of query aligns to 4:304/304 of 1iydA

query
sites
1iydA

F

Y

L

I

W

W

H

F

N

N

G

G

R

E

I

M

K

V

P

R

W

W

A

E

E

D

A

A

S

K

V

V

H

H

V

V

S

M

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

C

Y

Y

A

D

T

S

P

H

R

K

G

G

P

P

A

V

Y

V

F

F

R

R

L

H

A

R

D

E

H

H

T

M

R

Q

R
|
R

L

L

F

H

E

D

S

S

A

A

R

K

V

I

Y

Y

E

R

I

F

E

P

V

V

G

S

Y

Q

S

S

E

I

D

D

E

E

I

L

N

M

A

E

A

A

C

C

L

R

E

D

L

V

I

I

R

R

A

K

N

N

A

N

M

L

P

T

S

S

A

A

Y
|
Y

V

I

R

R

P

P

I

L

V

I

F

F

R

V

G

G

A

D

G

V

G

G

L

M

G

G

V

V

L

N

A

P

K

P

D

A

G

G

A

Y

P

S

V

T

E

D

V

V

A

I

I

I

M

A

A

A

M

F

A

P

W

W

G

G

A

A

Y
|
Y

L

L

G

G

-

A

E

E

A

A

A

L

E

E

R

Q

G

G

A

I

D

D

I

A

C

M

V

V

S

S

S

S

W

W

H

N

R

R

P

A

A

A

P

P

N

N

T

T

I

I

P

P

S

T

W

A

A

A

K
|
K

A
|
A

G

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

Q

L

L

L

I

V

G

G

L

S

E

E

A

A

R

R

R

R

N

H

G

G

Y

Y

D

Q

E

E

G

G

I

I

A

A

L

L

G

D

H

V

G

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N

N

L

L

F

F

L

E

V

V

K

K

N

D

G

G

R

V

L

L

L

F

T

T

P

P

P

P

S

F

S

T

A

S

G

S

I

A

L
|
L

A

P

G
|
G

I
|
I

T
|
T

R

R

D

D

S

A

V

I

L

I

A

K

L

L

A

A

A

K

G

E

L

L

G

G

I

I

A

E

V

V

E

R

E

E

R

Q

E

V

L

L

P

S

R

R

E

E

A

S

L

L

Y

Y

S

L

A

A

D

D

E

E

V

V

F

F

M

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

R

G

K

I

A

Q

V

V

G

G

K

E

G

G

A

R

P

C

G

G

P

P

V

V

T

T

Q

K

Q

R

L

I

R

Q

Q

Q

A

A

F

F

F

F

G

G

L

L

F

F

D

T

G

G

R

E

T

T

E

E

D

D

R

K

H

W

G

G

W

W

L

L

S

D

V

Q

V

V

N

N

active site: F33 (= F34), G35 (= G36), K156 (= K156), A157 (= A157), E190 (= E190), L214 (= L214)
binding glutaric acid: Y92 (= Y93), Y126 (= Y127), A255 (= A255)
binding pyridoxal-5'-phosphate: R56 (= R57), K156 (= K156), Y161 (= Y161), E190 (= E190), G193 (= G193), E194 (= E194), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), T254 (= T254)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
58% identity, 90% coverage: 5:304/333 of query aligns to 4:304/304 of 1i1mA

query
sites
1i1mA

F

Y

L

I

W

W

H

F

N

N

G

G

R

E

I

M

K

V

P

R

W

W

A

E

E

D

A

A

S

K

V

V

H

H

V

V

S

M

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F

F

E

E

G

G

E

I

R

R

V

C

Y

Y

A

D

T

S

P

H

R

K

G

G

P

P

A

V

Y

V

F

F

R

R

L

H

A

R

D

E

H

H

T

M

R

Q

R
|
R

L

L

F

H

E

D

S

S

A

A

R

K

V

I

Y

Y

E

R

I

F

E

P

V

V

G

S

Y

Q

S

S

E

I

D

D

E

E

I

L

N

M

A

E

A

A

C

C

L

R

E

D

L

V

I

I

R

R

A

K

N

N

A

N

M

L

P

T

S

S

A

A

Y
|
Y

V

I

R

R

P

P

I

L

V

I

F

F

R

V

G

G

A

D

G

V

G

G

L

M

G

G

V

V

L

N

A

P

K

P

D

A

G

G

A

Y

P

S

V

T

E

D

V

V

A

I

I

I

M

A

A

A

M

F

A

P

W

W

G

G

A

A

Y

Y

L

L

G

G

-

A

E

E

A

A

A

L

E

E

R

Q

G

G

A

I

D

D

I

A

C

M

V

V

S

S

S

S

W

W

H

N

R

R

P

A

A

A

P

P

N

N

T

T

I

I

P

P

S

T

W

A

A

A

K
|
K

A

A

G

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

Q

L

L

L

I

V

G

G

L

S

E

E

A

A

R

R

R

R

N

H

G

G

Y

Y

D

Q

E

E

G

G

I

I

A

A

L

L

G

D

H

V

G

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A

A

G
|
G

E
|
E

N

N

L

L

F

F

L

E

V

V

K

K

N

D

G

G

R

V

L

L

L

F

T

T

P

P

P

P

S

F

S

T

A

S

G

S

I

A

L
|
L

A

P

G
|
G

I
|
I

T
|
T

R

R

D

D

S

A

V

I

L

I

A

K

L

L

A

A

A

K

G

E

L

L

G

G

I

I

A

E

V

V

E

R

E

E

R

Q

E

V

L

L

P

S

R

R

E

E

A

S

L

L

Y

Y

S

L

A

A

D

D

E

E

V

V

F

F

M

M

T

S

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

R

G

K

I

A

Q

V

V

G

G

K

E

G

G

A

R

P

C

G

G

P

P

V

V

T

T

Q

K

Q

R

L

I

R

Q

Q

Q

A

A

F

F

F

F

G

G

L

L

F

F

D

T

G

G

R

E

T

T

E

E

D

D

R

K

H

W

G

G

W

W

L

L

S

D

V

Q

V

V

N

N

active site: K156 (= K156)
binding 4-methyl valeric acid: Y92 (= Y93), K156 (= K156), T254 (= T254), A255 (= A255)
binding pyridoxal-5'-phosphate: R56 (= R57), K156 (= K156), Y161 (= Y161), E190 (= E190), G193 (= G193), E194 (= E194), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
58% identity, 90% coverage: 5:304/333 of query aligns to 4:304/304 of 1i1lA

query
sites
1i1lA

F

Y

L

I

W

W

H

F

N

N

G

G

R

E

I

M

K

V

P

R

W

W

A

E

E

D

A

A

S

K

V

V

H

H

V

V

S

M

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F

F

E

E

G

G

E

I

R

R

V

C

Y

Y

A

D

T

S

P

H

R

K

G

G

P

P

A

V

Y

V

F

F

R

R

L

H

A

R

D

E

H

H

T

M

R

Q

R
|
R

L

L

F

H

E

D

S

S

A

A

R

K

V

I

Y

Y

E

R

I

F

E

P

V

V

G

S

Y

Q

S

S

E

I

D

D

E

E

I

L

N

M

A

E

A

A

C

C

L

R

E

D

L

V

I

I

R

R

A

K

N

N

A

N

M

L

P

T

S

S

A

A

Y
|
Y

V

I

R

R

P

P

I

L

V

I

F

F

R

V

G

G

A

D

G

V

G

G

L

M

G

G

V

V

L

N

A

P

K

P

D

A

G

G

A

Y

P

S

V

T

E

D

V

V

A

I

I

I

M

A

A

A

M

F

A

P

W

W

G

G

A

A

Y

Y

L

L

G

G

-

A

E

E

A

A

A

L

E

E

R

Q

G

G

A

I

D

D

I

A

C

M

V

V

S

S

S

S

W

W

H

N

R

R

P

A

A

A

P

P

N

N

T

T

I

I

P

P

S

T

W

A

A

A

K
|
K

A

A

G

G

G

G

N

N

Y
|
Y

L

L

S

S

S

S

Q

L

L

L

I

V

G

G

L

S

E

E

A

A

R

R

R

R

N

H

G

G

Y

Y

D

Q

E

E

G

G

I

I

A

A

L

L

G

D

H

V

G

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A

A

G
|
G

E

E

N

N

L

L

F

F

L

E

V

V

K

K

N

D

G

G

R

V

L

L

L

F

T

T

P

P

P

P

S

F

S

T

A

S

G

S

I

A

L

L

A

P

G
|
G

I
|
I

T
|
T

R

R

D

D

S

A

V

I

L

I

A

K

L

L

A

A

A

K

G

E

L

L

G

G

I

I

A

E

V

V

E

R

E

E

R

Q

E

V

L

L

P

S

R

R

E

E

A

S

L

L

Y

Y

S

L

A

A

D

D

E

E

V

V

F

F

M

M

T

S

G

G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

V

R

R

S

S

V

V

D

D

R

G

K

I

A

Q

V

V

G

G

K

E

G

G

A

R

P

C

G

G

P

P

V

V

T

T

Q

K

Q

R

L

I

R

Q

Q

Q

A

A

F

F

F

F

G

G

L

L

F

F

D

T

G

G

R

E

T

T

E

E

D

D

R

K

H

W

G

G

W

W

L

L

S

D

V

Q

V

V

N

N

active site: K156 (= K156)
binding 2-methylleucine: Y92 (= Y93), K156 (= K156), T254 (= T254), A255 (= A255)
binding pyridoxal-5'-phosphate: R56 (= R57), K156 (= K156), Y161 (= Y161), E190 (= E190), G193 (= G193), G216 (= G216), I217 (= I217), T218 (= T218), T254 (= T254)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
46% identity, 90% coverage: 5:304/333 of query aligns to 6:305/305 of 2ej0B

query
sites
2ej0B

F

L

L

I

W

W

H

M

N

N

G

G

R

A

I

F

K

V

P

P

W

Q

A

E

E

E

A

A

S

K

V

T

H

S

V

V

S

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

A

E

T

T

P

A

R

K

G

G

P

P

A

A

Y

I

F

F

R

R

L

L

A

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

E

N

S

S

A

A

R

K

V

V

Y

L

E

R

I

M

E

E

V

I

G

P

Y

F

S

A

E

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

L

K

E

E

L

V

I

V

R

R

A

R

N

N

A

G

M

Y

P

R

S

S

A

C

Y

Y

V

I

R

R

P

P

I

L

V

A

F

W

R

M

G

G

A

A

G

K

G

A

L

L

G

G

V

V

L

N

A

P

K

L

D

P

G

N

A

N

P

P

V

A

E

E

V

V

A

M

I

V

M

A

A

A

M

W

A

E

W

W

G

G

A

A

Y

Y

L

L

G

G

-

E

E

E

A

A

A

V

E

R

R

K

G

G

A

A

D

R

I

L

C

I

V

T

S

S

S

S

W

W

H

A

R

R

P

F

A

P

P

A

N

N

T

V

I

M

P

P

S

G

W

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

G

K

L

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

D

D

E

E

G

A

I

L

A

L

L

L

G

D

H

E

G

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

L

F

V

V

K

R

N

D

G

G

R

V

L

I

L

Y

T

A

P

L

P

E

S

H

S

S

A

V

G

N

I

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

D

D

S

S

V

V

L

I

A

R

L

I

A

A

A

K

G

D

L

L

G

G

I

Y

A

E

V

V

E

Q

E

V

R

V

E

R

L

A

P

T

R

R

E

D

A

Q

L

L

Y

Y

S

M

A

A

D

D

E

E

V

V

F

F

M

M

T

T

G
|
G

T
|
T

A

A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

K

R

A

P

V

I

G

G

K

K

G

G

A

T

P

A

G

G

P

P

V

V

T

A

Q

L

Q

R

L

L

R

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

R

R

T

R

E

P

D

E

R

Y

H

E

G

G

W

W

L

L

S

T

V

Y

V

V

N

N

active site: F35 (= F34), G37 (= G36), K158 (= K156), E192 (= E190), L215 (= L214)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R57), Y163 (= Y161), E192 (= E190), G195 (= G193), E196 (= E194), L215 (= L214), G217 (= G216), I218 (= I217), T219 (= T218), G254 (= G253), T255 (= T254)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
45% identity, 89% coverage: 6:303/333 of query aligns to 9:306/306 of 4whxA

query
sites
4whxA

L

I

W

W

H

M

N

D

G

G

R

K

I

L

K

I

P

E

W

W

A

R

E

D

A

A

S

K

V

I

H

H

V

V

S

L

T

T

H

H

A

T

L

L

H

H

Y

Y

G

G

S

M

S

G

V

V

F
|
F

E

E

G

G

E

V

R

R

V

A

Y

Y

A

K

T

T

P

A

R

D

G

G

-

G

P

T

A

A

Y

I

F

F

R

R

L

L

A

K

D

E

H

H

T

T

R

K

R

R

L

L

F

L

E

N

S

S

A

A

R

K

V

I

Y

F

E

Q

I

M

E

D

V

V

G

P

Y

F

S

D

E

Q

D

E

E

T

I

L

N

E

A

A

A

A

C

Q

L

R

E

D

L

V

I

V

R

R

A

E

N

N

A

K

M

L

P

E

S

S

A

C

Y
|
Y

V

L

R

R

P

P

I

I

V

I

F

W

R

I

G

G

A

S

G

E

G

K

L

L

G

G

V

V

L

S

A

A

K

K

D

-

G

G

A

N

P

T

V

I

E

H

V

V

A

A

I

I

M

A

A

A

M

W

A

P

W

W

G

G

A

A

Y

Y

L

L

G

G

E

E

A

E

A

G

-

L

E

A

R

K

G

G

A

I

D

R

I

V

C

K

V

T

S

S

S

S

W

F

H

T

R

R

P

H

A

H

P

V

N

N

T

V

I

S

P

M

S

V

W

R

A

A

K
|
K

A

A

G

S

G

G

N

W

Y

Y

L

V

S

N

S

S

Q

I

L

L

I

A

G

N

L

Q

E

E

A

A

R

T

R

A

N

D

G

G

Y

Y

D

D

E

E

G

A

I

L

A

L

L

L

G

D

H

V

G

D

G

G

L

Y

L

V

S

S

E
|
E

G

G

A

S

G

G

E

E

N

N

L

F

F

F

L

L

V

V

K

N

N

R

G

G

R

K

L

L

L

Y

T

T

P

P

P

-

S

D

S

L

A

A

G

S

I

C

L
|
L

A

D

G

G

I

I

T

T

R

R

D

D

S

T

V

V

L

I

A

T

L

L

A

A

A

K

G

E

L

A

G

G

I

I

A

E

V

V

E

I

E

E

R

K

E

R

L

I

P

T

R

R

E

D

A

E

L

V

Y

Y

S

T

A

A

D

D

E

E

V

A

F

F

M

F

T

T

G

G

T

T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

I

R

R

S

E

V

L

D

D

R

N

K

R

A

T

V

I

G

G

K

G

G

G

A

A

P

R

G

G

P

P

V

I

T

T

Q

E

Q

K

L

L

R

Q

Q

S

A

A

F

F

F

F

G

D

L

V

F

V

D

N

G

G

R

K

T

S

E

A

D

K

R

H

H

A

G

D

W

W

L

L

S

T

V

K

V

I

active site: F37 (= F34), K160 (= K156), E194 (= E190), L217 (= L214)
binding alanine: Y97 (= Y93), K160 (= K156), A258 (= A255)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
44% identity, 90% coverage: 5:304/333 of query aligns to 6:297/297 of 2ej3A

query
sites
2ej3A

F

L

L

I

W

W

H

M

N

N

G

G

R

A

I

F

K

V

P

P

W

Q

A

E

E

E

A

A

S

K

V

T

H

S

V

V

S

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

A

E

T

T

P

A

R

K

G

G

P

P

A

A

Y

I

F

F

R

R

L

L

A

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

E

N

S

S

A

A

R

K

V

V

Y

L

E

R

I

M

E

E

V

I

G

P

Y

F

S

A

E

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

L

K

E

E

L

V

I

V

R

R

A

R

N

N

A

G

M

Y

P

R

S

S

A

C

Y

Y

V

I

R

R

P

P

I

L

V

A

F

W

R

M

G

G

A

A

G

K

G

A

L

L

G

G

V

V

L

N

A

P

K

L

D

P

G

N

A

N

P

P

V

A

E

E

V

V

A

M

I

V

M

A

A

A

M

W

A

E

W

W

G

-

A

-

Y

-

L

-

G

-

E

-

A

-

A

V

E

R

R

K

G

G

A

A

D

R

I

L

C

I

V

T

S

S

S

S

W

W

H

A

R

R

P

F

A

P

P

A

N

N

T

V

I

M

P

P

S

G

W

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

G

K

L

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

D

D

E

E

G

A

I

L

A

L

L

L

G

D

H

E

G

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E

E

N

N

L

L

F

F

L

F

V

V

K

R

N

D

G

G

R

V

L

I

L

Y

T

A

P

L

P

E

S

H

S

S

A

V

G

N

I

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

D

D

S

S

V

V

L

I

A

R

L

I

A

A

A

K

G

D

L

L

G

G

I

Y

A

E

V

V

E

Q

E

V

R

V

E

R

L

A

P

T

R

R

E

D

A

Q

L

L

Y

Y

S

M

A

A

D

D

E

E

V

V

F

F

M

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

K

R

A

P

V

I

G

G

K

K

G

G

A

T

P

A

G

G

P

P

V

V

T

A

Q

L

Q

R

L

L

R

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

R

R

T

R

E

P

D

E

R

Y

H

E

G

G

W

W

L

L

S

T

V

Y

V

V

N

N

active site: F35 (= F34), G37 (= G36), K150 (= K156), E184 (= E190), L207 (= L214)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G193), G246 (= G253), T247 (= T254), A248 (= A255)
binding pyridoxal-5'-phosphate: R58 (= R57), K150 (= K156), Y155 (= Y161), E184 (= E190), G187 (= G193), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
44% identity, 90% coverage: 5:304/333 of query aligns to 6:297/297 of 2eiyA

query
sites
2eiyA

F

L

L

I

W

W

H

M

N

N

G

G

R

A

I

F

K

V

P

P

W

Q

A

E

E

E

A

A

S

K

V

T

H

S

V

V

S

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

A

E

T

T

P

A

R

K

G

G

P

P

A

A

Y

I

F

F

R

R

L

L

A

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

E

N

S

S

A

A

R

K

V

V

Y

L

E

R

I

M

E

E

V

I

G

P

Y

F

S

A

E

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

L

K

E

E

L

V

I

V

R

R

A

R

N

N

A

G

M

Y

P

R

S

S

A

C

Y
|
Y

V

I

R

R

P

P

I

L

V

A

F

W

R

M

G

G

A

A

G

K

G

A

L

L

G

G

V

V

L

N

A

P

K

L

D

P

G

N

A

N

P

P

V

A

E

E

V

V

A

M

I

V

M

A

A

A

M

W

A

E

W

W

G

-

A

-

Y

-

L

-

G

-

E

-

A

-

A

V

E

R

R

K

G

G

A

A

D

R

I

L

C

I

V

T

S

S

S

S

W

W

H

A

R

R

P

F

A

P

P

A

N

N

T

V

I

M

P

P

S

G

W

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

G

K

L

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

D

D

E

E

G

A

I

L

A

L

L

L

G

D

H

E

G

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

L

F

V

V

K

R

N

D

G

G

R

V

L

I

L

Y

T

A

P

L

P

E

S

H

S

S

A

V

G

N

I

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

D

D

S

S

V

V

L

I

A

R

L

I

A

A

A

K

G

D

L

L

G

G

I

Y

A

E

V

V

E

Q

E

V

R

V

E

R

L

A

P

T

R

R

E

D

A

Q

L

L

Y

Y

S

M

A

A

D

D

E

E

V

V

F

F

M

M

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

K

R

A

P

V

I

G

G

K

K

G

G

A

T

P

A

G

G

P

P

V

V

T

A

Q

L

Q

R

L

L

R

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

R

R

T

R

E

P

D

E

R

Y

H

E

G

G

W

W

L

L

S

T

V

Y

V

V

N

N

active site: F35 (= F34), G37 (= G36), K150 (= K156), E184 (= E190), L207 (= L214)
binding 4-methyl valeric acid: F35 (= F34), Y94 (= Y93), T247 (= T254), A248 (= A255)
binding pyridoxal-5'-phosphate: R58 (= R57), K150 (= K156), Y155 (= Y161), E184 (= E190), G187 (= G193), E188 (= E194), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
44% identity, 90% coverage: 5:304/333 of query aligns to 6:297/297 of 1wrvA

query
sites
1wrvA

F

L

L

I

W

W

H

M

N

N

G

G

R

A

I

F

K

V

P

P

W

Q

A

E

E

E

A

A

S

K

V

T

H

S

V

V

S

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

A

E

T

T

P

A

R

K

G

G

P

P

A

A

Y

I

F

F

R

R

L

L

A

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

E

N

S

S

A

A

R

K

V

V

Y

L

E

R

I

M

E

E

V

I

G

P

Y

F

S

A

E

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

L

K

E

E

L

V

I

V

R

R

A

R

N

N

A

G

M

Y

P

R

S

S

A

C

Y

Y

V

I

R

R

P

P

I

L

V

A

F

W

R

M

G

G

A

A

G

K

G

A

L

L

G

G

V

V

L

N

A

P

K

L

D

P

G

N

A

N

P

P

V

A

E

E

V

V

A

M

I

V

M

A

A

A

M

W

A

E

W

W

G

-

A

-

Y

-

L

-

G

-

E

-

A

-

A

V

E

R

R

K

G

G

A

A

D

R

I

L

C

I

V

T

S

S

S

S

W

W

H

A

R

R

P

F

A

P

P

A

N

N

T

V

I

M

P

P

S

G

W

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

G

K

L

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

D

D

E

E

G

A

I

L

A

L

L

L

G

D

H

E

G

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E

E

N

N

L

L

F

F

L

F

V

V

K

R

N

D

G

G

R

V

L

I

L

Y

T

A

P

L

P

E

S

H

S

S

A

V

G

N

I

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

D

D

S

S

V

V

L

I

A

R

L

I

A

A

A

K

G

D

L

L

G

G

I

Y

A

E

V

V

E

Q

E

V

R

V

E

R

L

A

P

T

R

R

E

D

A

Q

L

L

Y

Y

S

M

A

A

D

D

E

E

V

V

F

F

M

M

T

T

G

G

T
|
T

A

A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

K

R

A

P

V

I

G

G

K

K

G

G

A

T

P

A

G

G

P

P

V

V

T

A

Q

L

Q

R

L

L

R

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

R

R

T

R

E

P

D

E

R

Y

H

E

G

G

W

W

L

L

S

T

V

Y

V

V

N

N

active site: F35 (= F34), G37 (= G36), K150 (= K156), E184 (= E190), L207 (= L214)
binding pyridoxal-5'-phosphate: R58 (= R57), K150 (= K156), Y155 (= Y161), E184 (= E190), G187 (= G193), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), T247 (= T254)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
44% identity, 90% coverage: 5:304/333 of query aligns to 6:294/294 of 2ej2A

query
sites
2ej2A

F

L

L

I

W

W

H

M

N

N

G

G

R

A

I

F

K

V

P

P

W

Q

A

E

E

E

A

A

S

K

V

T

H

S

V

V

S

L

T

S

H

H

A

A

L

L

H

H

Y

Y

G

G

S

T

S

S

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

A

E

T

T

P

A

R

K

G

G

P

P

A

A

Y

I

F

F

R

R

L

L

A

K

D

E

H

H

T

V

R

K

R
|
R

L

F

F

Y

E

N

S

S

A

A

R

K

V

V

Y

L

E

R

I

M

E

E

V

I

G

P

Y

F

S

A

E

P

D

E

E

E

I

L

N

E

A

E

A

A

C

I

L

K

E

E

L

V

I

V

R

R

A

R

N

N

A

G

M

Y

P

R

S

S

A

C

Y
|
Y

V

I

R

R

P

P

I

L

V

A

F

W

R

M

G

G

A

A

G

K

G

A

L

L

G

G

V

V

L

N

A

P

K

L

D

P

G

N

A

N

P

P

V

A

E

E

V

V

A

M

I

V

M

A

A

A

M

W

A

E

W

W

G

G

A

A

Y

R

L

L

G

-

E

-

A

-

A

-

E

-

R

-

G

-

A

-

D

-

I

-

C

I

V

T

S

S

S

S

W

W

H

A

R

R

P

F

A

P

P

A

N

N

T

V

I

M

P

P

S

G

W

K

A

A

K
|
K

A

V

G

G

G

G

N

N

Y
|
Y

L

V

S

N

S

S

Q

A

L

L

I

A

G

K

L

M

E

E

A

A

R

V

R

A

N

A

G

G

Y

A

D

D

E

E

G

A

I

L

A

L

L

L

G

D

H

E

G

E

G

G

L

Y

L

V

S

A

E
|
E

G

G

A

S

G
|
G

E
|
E

N

N

L

L

F

F

L

F

V

V

K

R

N

D

G

G

R

V

L

I

L

Y

T

A

P

L

P

E

S

H

S

S

A

V

G

N

I

-

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

D

D

S

S

V

V

L

I

A

R

L

I

A

A

A

K

G

D

L

L

G

G

I

Y

A

E

V

V

E

Q

E

V

R

V

E

R

L

A

P

T

R

R

E

D

A

Q

L

L

Y

Y

S

M

A

A

D

D

E

E

V

V

F

F

M

M

T

T

G

G

T
|
T

A
|
A

A

A

E

E

I

V

T

T

P

P

V

V

R

S

S

M

V

I

D

D

R

W

K

R

A

P

V

I

G

G

K

K

G

G

A

T

P

A

G

G

P

P

V

V

T

A

Q

L

Q

R

L

L

R

R

Q

E

A

V

F

Y

F

L

G

E

L

A

F

V

D

T

G

G

R

R

T

R

E

P

D

E

R

Y

H

E

G

G

W

W

L

L

S

T

V

Y

V

V

N

N

active site: F35 (= F34), G37 (= G36), K147 (= K156), E181 (= E190), L204 (= L214)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R57), Y94 (= Y93), Y152 (= Y161), E181 (= E190), G184 (= G193), E185 (= E194), L204 (= L214), G206 (= G216), I207 (= I217), T208 (= T218), T244 (= T254), A245 (= A255)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
40% identity, 89% coverage: 6:301/333 of query aligns to 9:298/301 of 6thqB

query
sites
6thqB

L

V

W

W

H

L

N

D

G

G

R

R

I

L

K

V

P

D

W

E

A

E

E

E

A

A

S

K

V

V

H

T

V

V

S

L

T

S

H

P

A

S

L

L

H

N

Y

Y

G

G

S

F

S

G

V

V

F
|
F

E

E

G

G

E

I

R

R

V

A

Y

Y

A

W

T

N

P

G

R

E

G

N

P

L

A

Y

Y

V

F

F

R

R

L

L

A

R

D

D

H

H

T

M

R

E

R
|
R

L

L

F

L

E

R

S

S

A

A

R

K

V

I

Y

I

E

G

I

L

E

D

V

V

G

P

Y

Y

S

T

E

A

D

E

E

E

I

L

N

S

A

K

A

A

C

V

L

V

E

E

L

T

I

V

R

R

A

A

N

N

A

G

M

F

P

K

-

E

S

D

A

L

Y
|
Y

V

I

R

R

P

P

I

V

V

A

F

Y

R

I

G

S

A

K

G

P

G

Q

L

I

G

S

V

L

L

D

A

V

K

R

D

-

G

G

A

L

P

Q

V

A

E

S

V

V

A

A

I

I

M

A

A

A

M

I

A

P

W

F

G

G

A

K

Y

Y

L

L

G

K

E

V

A

E

A

G

E

V

R

R

G

A

A

A

D

-

I

-

C

-

V

V

S

V

S

S

W

W

H

R

R

R

P

V

A

H

P

T

N

S

T

M

I

M

P

P

S

V

W

M

A

A

K
|
K

A

A

G

T

G

G

N

I

Y
|
Y

L

L

S

N

S

S

Q

I

L

M

I

A

G

A

L

V

E

E

A

A

R

R

R

A

N

R

G

G

Y

Y

D

D

E

E

G

A

I

I

A

M

L

L

G

N

H

A

G

E

G

G

L

K

L

V

S

V

E
|
E

G

G

A

S

G
|
G

E
|
E

N
|
N

L

I

F

F

L

I

V

V

K

R

N

R

G

G

R

V

L

L

L

M

T

T

P

P

P

P

S

L

S

E

A

D

G

G

I

I

L
|
L

A

E

G
|
G

I
|
I

T
|
T

R

R

D

E

S

T

V

V

L

I

A

S

L

I

A

A

A

G

G

D

L

L

G

G

I

I

A

P

V

L

E

L

E

E

R

K

E

S

L

I

P

T

R

R

E

E

A

E

L

L

Y

Y

S

A

A

A

D

D

E

E

V

A

F

F

M

F

T

V

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

T

P

P

V

I

R

I

S

E

V

I

D

D

R

G

K

R

A

V

V

L

G

Q

K

R

G

-

A

-

P

-

G

G

P

P

V

I

T

T

Q

Q

Q

K

L

I

R

A

Q

E

A

T

F

Y

F

R

G

R

L

I

F

V

D

L

G

G

R

K

T

E

E

E

D

K

R

Y

H

L

G

P

W

W

L

L

S

T

active site: F37 (= F34), K156 (= K156), E190 (= E190), L214 (= L214)
binding pyridoxal-5'-phosphate: R60 (= R57), K156 (= K156), Y161 (= Y161), E190 (= E190), N195 (= N195), L214 (= L214), G216 (= G216), I217 (= I217), T218 (= T218), T254 (= T254)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R57), Y97 (= Y93), K156 (= K156), Y161 (= Y161), E190 (= E190), G193 (= G193), E194 (= E194), N195 (= N195), G216 (= G216), I217 (= I217), T218 (= T218), G253 (= G253), T254 (= T254), A255 (= A255)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
41% identity, 84% coverage: 5:285/333 of query aligns to 4:278/290 of 5e25A

query
sites
5e25A

F

L

L

V

W

Y

H

M

N

N

G

G

R

E

I

F

K

V

P

P

W

E

A

S

E

Q

A

A

S

K

V

V

H

S

V

V

S

F

T

D

H

H

A

G

L

F

H

L

Y

Y

G

G

S

D

S

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

A

-

T

-

P

-

R

-

G

N

P

G

A

K

Y

V

F

F

R

K

L

L

A

Y

D

E

H

H

T

I

R

D

R
|
R

L

L

F

Y

E

D

S

C

A

A

R

R

V

V

Y

I

E

D

I

L

E

K

V

I

G

P

Y

L

S

S

E

K

D

E

E

E

I

F

N

A

A

E

A

A

C

I

L

L

E

E

L

T

I

L

R

R

A

R

N

N

A

N

M

L

P

R

S

D

A

A

Y
|
Y

V

I

R

R

P

P

I

I

V

V

F

T

R

R

G

G

A

A

G

G

G

D

L

L

G

G

V

L

L

D

A

P

K

R

D

K

G

C

A

P

P

S

V

P

E

N

V

V

A

I

I

I

M

I

A

T

M

K

A

P

W

W

G

G

A

K

Y

L

L

Y

G

G

E

D

A

L

A

Y

E

E

R

K

G

G

A

L

D

K

I

A

C

I

V

T

S

V

S

A

W

I

H

R

R

R

P

N

A

A

P

I

N

D

T

S

I

L

P

P

S

P

W

N

A

I

K
|
K

A

S

G

-

G

L

N

N

Y
|
Y

L

L

S

N

S

N

Q

I

L

L

I

A

G

K

L

I

E

E

A

A

R

N

R

A

N

K

G

G

Y

G

D

D

E

E

G

A

I

I

A

F

L

L

G

D

H

H

G

N

G

G

L

Y

L

I

S

S

E
|
E

G

G

A

S

G
|
G

E
x
D

N

N

L

I

F

F

L

I

V

V

K

K

N

N

G

G

R

T

L

I

L

T

T

T

P

P

P

P

S

T

S

L

A

N

G

N

I

-

L
|
L

A

K

G
|
G

I
|
I

T
|
T

R

R

D

Q

S

V

V

V

L

I

A

E

L

L

A

I

A

N

G

E

L

L

G

E

I

I

A

P

V

F

E

R

E

E

R

A

E

N

L

I

P

G

R

L

E

F

A

D

L

L

Y

Y

S

S

A

A

D

D

E

E

V

I

F

F

M

V

T

T

G
|
G

T
|
T

A
|
A

A

A

E

E

I

I

T

A

P

P

V

V

R

T

S

Y

V

I

D

D

R

G

K

R

A

T

V

V

G

G

K

N

G

G

A

K

P

P

G

G

P

K

V

V

T

T

Q

K

Q

M

L

L

R

M

Q

E

A

K

F

F

active site: F33 (= F34), G35 (= G36), K151 (= K156), E184 (= E190), L207 (= L214)
binding 2-oxoglutaric acid: Y88 (= Y93), K151 (= K156), T247 (= T254), A248 (= A255)
binding pyridoxal-5'-phosphate: R52 (= R57), K151 (= K156), Y155 (= Y161), E184 (= E190), G187 (= G193), D188 (≠ E194), L207 (= L214), G209 (= G216), I210 (= I217), T211 (= T218), G246 (= G253), T247 (= T254)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
40% identity, 91% coverage: 2:303/333 of query aligns to 2:304/307 of 6q8eA

query
sites
6q8eA

N

N

T

P

P

K

F

Y

L

L

W

W

H

W

N

N

G

H

R

R

I

I

K

I

P

P

W

W

A

E

E

E

A

A

S

T

V

V

H

H

V

L

S

T

T

D

H

Y

A

W

L

W

H

A

Y

S

G

V

S

T

S

A

V

V

F
|
F

E

E

G

G

E

I

R

R

V

G

Y

Y

-

W

A

N

T

N

P

A

R

E

G

G

P

E

A

M

Y

Y

-

I

F

F

R

R

L

L

A

E

D

D

H

H

T

A

R

R

R
|
R

L

L

F

E

E

Q

S

S

A

M

R

Q

V

L

Y

I

E

R

I

M

E

P

V

K

G

E

Y

F

S

T

E

V

D

D

E

E

I

I

N

C

A

Q

A

A

C

T

L

I

E

D

L

L

I

V

R

R

A

A

N

N

A

D

M

Y

P

R

-

G

S

D

A

V

Y

Y

V

I

R

M

P

P

I

L

V

A

F

Y

R

-

G

A

A

V

G

G

G

N

L

K

G

A

V

F

L

S

A

V

K

V

D

G

G

D

A

R

P

T

V

T

E

E

V

M

A

F

I

I

M

Y

A

S

M

R

A

P

W

A

G

V

A

S

Y

R

L

L

G

E

E

E

A

D

A

F

E

S

R

L

G

H

A

A

D

-

I

-

C

C

V

Y

S

S

S

S

W

W

H

T

R

R

P

I

A

N

P

E

N

R

T

V

I

L

P

P

S

P

W

R

A

I

K
|
K

A

A

G

L

G

A

N

N

Y
|
Y

L

R

S

N

S

S

Q

Q

L

L

I

A

G

S

L

S

E

E

A

A

R

A

R

M

N

N

G

G

Y

Y

D

D

E

T

G

A

I

L

A

F

L

L

G

N

H

P

G

E

G

G

L

K

L

V

S

A

E
|
E

G

G

A

T

G
|
G

E
x
S

N
x
C

L

V

F

F

L

F

V

V

K

R

N

K

G

G

R

K

L

L

L

I

T

T

P

P

P

D

S

I

S

T

A

S

G

G

I

I

L
|
L

A

E

G
x
S

I
|
I

T
|
T

R

R

D

D

S

T

V

V

L

I

A

H

L

L

A

A

A

R

G

E

-

V

L

L

G

G

I

L

A

E

V

V

E

E

E

E

R

R

E

V

L

V

P

D

R

R

E

T

A

E

L

T

Y

Y

S

L

A

A

D

D

E

E

V

A

F

F

M

L

T

C

G
|
G

T
|
T

A

H

A

A

E

E

I

I

T

T

P

P

V

I

R

A

S

S

V

I

D

D

R

R

K

H

A

E

V

M

G

K

K

H

G

G

A

A

P

P

G

G

P

P

V

I

T

T

Q

R

Q

Q

L

L

R

R

Q

D

A

I

F

Y

F

R

G

E

L

V

F

V

D

Y

G

G

R

R

T

D

E

F

D

R

R

Y

H

R

G

N

W

W

L

L

S

T

V

P

V

V

active site: F34 (= F34), K156 (= K156), E190 (= E190), L214 (= L214)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R57), K156 (= K156), Y161 (= Y161), E190 (= E190), G193 (= G193), S194 (≠ E194), C195 (≠ N195), L214 (= L214), S216 (≠ G216), I217 (= I217), T218 (= T218), G254 (= G253), T255 (= T254)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
37% identity, 85% coverage: 5:288/333 of query aligns to 3:280/290 of 5mr0D

query
sites
5mr0D

F

Y

L

V

W

Y

H

M

N

D

G

G

R

E

I

F

K

V

P

P

W

E

A

N

E

E

A

A

S

K

V

V

H

S

V

I

S

F

T

D

H

H

A

G

L

F

H

L

Y

Y

G

G

S

D

S

G

V

V

F
|
F

E

E

G
|
G

E

I

R

R

V

A

Y

Y

A

-

T

-

P

-

R

-

G

N

P

G

A

R

Y

V

F

F

R

R

L

L

A

K

D

E

H

H

T

I

R

D

R
|
R

L

L

F

Y

E

D

S

S

A

A

R

K

V

A

Y

I

E

D

I

L

E

E

V

I

G

P

Y

I

S

T

E

K

D

E

E

E

I

F

N

M

A

E

A

I

C

I

L

L

E

E

L

T

I

L

R

R

A

K

N

N

A

N

M

L

P

R

S

D

A

A

Y

Y

V

I

R

R

P

P

I

I

V

V

F

T

R

R

G

G

A

I

G

G

G

D

L

L

G
|
G

V
x
L

L

D

A

P

K

R

D

K

G

C

A

Q

P

N

V

P

E

S

V

I

A

I

I

V

M

I

A

T

M

K

A

P

W

W

G

G

A

K

Y

L

L

Y

G

G

E

D

A

L

A

Y

E

E

R

K

G

G

A

L

D

T

I

A

C

I

V

T

S

V

S

A

W

V

H

R

R

R

P

N

A

S

P

F

N

D

T

A

I

L

P

P

S

P

W

N

A

I

K
|
K

A

S

G

L

G

-

N

N

Y
|
Y

L

L

S

N

S

N

Q

I

L

L

I

A

G

K

L

I

E

E

A

A

R

N

R

A

N

K

G

G

Y

G

D

D

E

E

G

A

I

I

A

F

L

L

G

D

H

R

G

N

G

G

L

Y

L

V

S

S

E
|
E

G

G

A

S

G
|
G

E
x
D

N

N

L

I

F

F

L

V

V

V

K

K

N

N

G

G

R

A

L

I

L

T

T

T

P

P

P

P

S

T

S

I

A

N

G

N

I

-

L
|
L

A

R

G

G

I
|
I

T
|
T

R

R

D

E

S

A

V

V

L

I

A

E

L

I

A

I

A

N

G

R

L

L

G

G

I

I

A

P

V

F

E

K

E

E

R

T

E

N

L

I

P

G

R

L

E

Y

A

D

L

L

Y

Y

S

T

A

A

D

D

E

E

V

V

F

F

M

V

T

T

G
|
G

T
|
T

A

A

A

A

E

E

I

I

T

A

P

P

V

I

R

V

S

V

V

I

D

D

R

G

K

R

A

K

V

I

G

G

K

D

G

G

A

K

P

P

G

G

P

E

V

I

T

T

Q

R

Q

K

L

L

R

M

Q

E

A

E

F

F

F

S

G

K

L

L

active site: F32 (= F34), G34 (= G36), K150 (= K156), E183 (= E190), L206 (= L214)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R57), G100 (= G106), L101 (≠ V107), K150 (= K156), Y154 (= Y161), E183 (= E190), G186 (= G193), D187 (≠ E194), L206 (= L214), I209 (= I217), T210 (= T218), G245 (= G253), T246 (= T254)

6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
38% identity, 88% coverage: 7:300/333 of query aligns to 8:303/308 of 6h65C

query
sites
6h65C

W

W

H

M

N

N

G

G

R

Q

I

C

K

I

P

P

W

W

A

D

E

Q

A

C

S

S

V

L

H

H

V

V

S

S

T

T

H

Q

A

A

L

A

H

F

Y

F

G

G

S

A

S

S

V

L

F
|
F

E

E

G

G

E

V

R

R

V

A

Y

Y

-

W

A

N

T

A

P

E

R

R

G

E

P

Q

A

L

Y

Y

-

V

F

F

R

R

L

L

A

D

D

E

H

H

T

L

R

R

R
|
R

L

L

F

E

E

Q

S

S

A

A

R

K

V

M

Y

L

E

R

I

M

E

K

V

L

G

S

Y

M

S

P

E

I

D

A

E

D

I

I

N

R

A

Q

A

G

C

V

L

L

E

E

L

L

I

L

R

R

A

A

N

N

A

E

M

F

P

R

S

S

A

-

Y

-

V

-

R

-

P