SitesBLAST
Comparing WP_027721936.1 NCBI__GCF_000425265.1:WP_027721936.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
55% identity, 97% coverage: 7:339/342 of query aligns to 6:346/346 of Q04797
- S98 (= S99) modified: Phosphoserine
- Y146 (≠ A147) modified: Phosphotyrosine
4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/359 of 4r5hA
- binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), K222 (= K215), R244 (= R237), H251 (= H244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), T94 (≠ S98), P125 (= P129), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ Q165), G328 (= G321)
4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/359 of 4r4jA
- binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212), K222 (= K215), R244 (= R237), H251 (= H244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), T94 (≠ S98), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ Q165), G328 (= G321)
3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/359 of 3pyxB
- binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R102), G158 (= G162), E219 (= E212), K222 (= K215), R244 (= R237)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), C127 (= C131), S157 (= S161), G158 (= G162), G160 (= G164), M161 (≠ Q165), N324 (= N317), L325 (≠ I318)
3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/358 of 3q11A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), A71 (= A75), T75 (= T79), G160 (= G164), M161 (≠ Q165), G162 (≠ K166)
- binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R102), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212), K222 (= K215), R244 (= R237)
4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/360 of 4r51A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (≠ S39), S36 (= S40), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), N93 (= N97), T94 (≠ S98), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ Q165), G328 (= G321)
- binding phthalic acid: S73 (≠ G77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), K222 (= K215)
4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 4r54A
- binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G76), S73 (≠ G77), T94 (≠ S98), S95 (= S99), R98 (= R102), K222 (= K215)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), T94 (≠ S98), N126 (= N130), C127 (= C131), G160 (= G164), G328 (= G321)
4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 4r41A
- binding 4-nitro-2-phosphonobenzoic acid: S70 (= S74), G72 (= G76), S73 (≠ G77), N93 (= N97), T94 (≠ S98), S95 (= S99), R98 (= R102), K222 (= K215)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), A71 (= A75), G160 (= G164), M161 (≠ Q165), G162 (≠ K166)
4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 4r3nA
- active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
- binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G77), T94 (≠ S98), S95 (= S99), R98 (= R102), N126 (= N130), K222 (= K215)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (≠ S39), S36 (= S40), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), N93 (= N97), T94 (≠ S98), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ Q165), G328 (= G321)
3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 3q1lA
3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 3pwsA
- binding (2R)-2-aminohexanedioic acid: R98 (= R102), N126 (= N130), G158 (= G162), I208 (= I201), E219 (= E212), K222 (= K215), R244 (= R237)
- binding adenosine-2'-5'-diphosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), A71 (= A75), T75 (= T79), G160 (= G164), M161 (≠ Q165)
3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 3pwkA
- binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), A71 (= A75), T75 (= T79), G160 (= G164)
- binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R102), N126 (= N130), G158 (= G162), A159 (≠ T163), E219 (= E212), K222 (= K215), R244 (= R237)
2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 2gz3A
- active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
- binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E212), R244 (= R237), H251 (= H244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), G158 (= G162), G160 (= G164), M161 (≠ Q165), N324 (= N317), A329 (= A322)
2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 2gz2A
- active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
- binding adenosine-2'-5'-diphosphate: G8 (= G12), T10 (= T14), G11 (= G15), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), A71 (= A75), T75 (= T79)
2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/357 of 2gz1A
- active site: C127 (= C131), Q154 (= Q158), R244 (= R237), H251 (= H244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G12), T10 (= T14), G11 (= G15), A12 (= A16), V13 (= V17), A35 (≠ S39), S36 (= S40), R38 (= R42), S39 (= S43), T56 (≠ L60), S70 (= S74), A71 (= A75), G72 (= G76), T75 (= T79), N93 (= N97), S157 (= S161), G158 (= G162), G160 (= G164), M161 (≠ Q165), N324 (= N317), L325 (≠ I318)
3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
55% identity, 97% coverage: 8:340/342 of query aligns to 4:347/361 of 3pylC
P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
52% identity, 96% coverage: 8:337/342 of query aligns to 7:335/337 of P23247
- C132 (= C131) active site, Acyl-thioester intermediate
2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
52% identity, 96% coverage: 8:337/342 of query aligns to 6:334/336 of 2r00C
3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
43% identity, 96% coverage: 8:335/342 of query aligns to 4:342/342 of 3tz6A
- active site: C129 (= C131), Q156 (= Q158), R248 (= R237), H255 (= H244)
- binding cysteine: C129 (= C131), Q156 (= Q158), G160 (= G162), E223 (= E212), R248 (= R237), H255 (= H244)
- binding glycerol: S108 (≠ P112), G187 (vs. gap), F192 (vs. gap), P201 (≠ Q192), Q225 (≠ M214), R228 (≠ V217), F229 (≠ N218), Q335 (= Q328), E338 (= E331), L339 (≠ T332)
- binding sulfate ion: R98 (= R102), H117 (≠ D120), R119 (≠ H122), N128 (= N130), C129 (= C131), K226 (= K215), E270 (= E259), R273 (= R262)
Q57658 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
29% identity, 96% coverage: 7:336/342 of query aligns to 10:352/354 of Q57658
Query Sequence
>WP_027721936.1 NCBI__GCF_000425265.1:WP_027721936.1
MSTKNPRVAVVGATGAVGREMLKVLEQRNFPASEVVPFSSSRSAGTKVPYKDEELTVIEL
KEDSFEGFDIALFSAGGSTSEKFAPLAAKAGCVVVDNSSAWRMDPKVPLVVPEVNPEDLD
WHPGIIANPNCSTIQMVVALKPIHDAAKIKRIIVSTYQAVSGTGQKAITELETQVSRLFN
NRDVVADVYPHQIAFNCLPHIDVFMDNGYTKEEMKMVNETRKIMGDDSIKVTATAVRVPV
FYSHSESVNIETEEKVTVEECRNMLANFPGITVIDFPEKHHYPMAIDCAGEDDVYVGRIR
EDETIENGLNMWIVSDNIRKGAALNTVQIAETLIARDLVRVK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory