SitesBLAST
Comparing WP_027722326.1 NCBI__GCF_000425265.1:WP_027722326.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
51% identity, 99% coverage: 1:394/396 of query aligns to 1:396/408 of P38502
- N7 (= N7) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (= S10) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (= S12) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (= K14) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R89) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V91) mutation to A: Decreases affinity for acetate.
- L122 (= L120) mutation to A: Decreases affinity for acetate.
- D148 (= D146) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (≠ A177) mutation to A: Decreases affinity for acetate.
- N211 (= N209) mutation to A: Slightly reduced enzyme activity.
- P232 (= P230) mutation to A: Decreases affinity for acetate.
- R241 (= R239) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (= E382) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
1tuuB Acetate kinase crystallized with atpgs (see paper)
51% identity, 99% coverage: 1:394/396 of query aligns to 1:396/398 of 1tuuB
- active site: N7 (= N7), R91 (= R89), H180 (= H178), R241 (= R239), E384 (= E382)
- binding adenosine monophosphate: D283 (= D280), R285 (= R282), G331 (= G328), I332 (= I329), N335 (= N332), S336 (≠ D333)
- binding trihydrogen thiodiphosphate: H180 (= H178), G212 (= G210), R241 (= R239)
1tuuA Acetate kinase crystallized with atpgs (see paper)
51% identity, 99% coverage: 1:394/396 of query aligns to 1:396/399 of 1tuuA
- active site: N7 (= N7), R91 (= R89), H180 (= H178), R241 (= R239), E384 (= E382)
- binding adenosine-5'-diphosphate: K14 (= K14), G210 (= G208), D283 (= D280), F284 (≠ L281), R285 (= R282), G331 (= G328), I332 (= I329), N335 (= N332)
- binding sulfate ion: R91 (= R89), H180 (= H178), G212 (= G210)
7fj9A Kpacka (pduw) with amppnp complex structure
44% identity, 99% coverage: 2:393/396 of query aligns to 3:391/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
44% identity, 99% coverage: 2:393/396 of query aligns to 3:391/395 of 7fj8A
4iz9A Crystal structure of an acetate kinase from mycobacterium avium bound to an unknown acid-apcpp conjugate and manganese (see paper)
43% identity, 99% coverage: 2:395/396 of query aligns to 5:380/381 of 4iz9A
- active site: N10 (= N7), R74 (= R89), H163 (= H178), R224 (= R239), E367 (= E382)
- binding diphosphomethylphosphonic acid adenosyl ester: K17 (= K14), G193 (= G208), N194 (= N209), D265 (= D280), F266 (≠ L281), R267 (= R282), G313 (= G328), I314 (= I329), N317 (= N332), D318 (= D333)
4ijnA Crystal structure of an acetate kinase from mycobacterium smegmatis bound to amp and sulfate (see paper)
42% identity, 99% coverage: 1:393/396 of query aligns to 2:376/376 of 4ijnA
- active site: N8 (= N7), R72 (= R89), H161 (= H178), R222 (= R239), E365 (= E382)
- binding adenosine monophosphate: G191 (= G208), N192 (= N209), D263 (= D280), F264 (≠ L281), R265 (= R282), G311 (= G328), V312 (≠ I329), N315 (= N332), V316 (≠ D333)
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
43% identity, 99% coverage: 3:393/396 of query aligns to 4:389/394 of 4fwsA
- active site: N8 (= N7), R83 (= R89), H172 (= H178), R233 (= R239), E378 (= E382)
- binding cytidine-5'-triphosphate: G202 (= G208), N203 (= N209), G204 (= G210), D275 (= D280), L276 (= L281), R277 (= R282), G323 (= G328), I324 (= I329), N327 (= N332)
- binding 1,2-ethanediol: V21 (≠ M20), C24 (≠ G23), H115 (= H121), N203 (= N209), T232 (= T238), R233 (= R239), K262 (≠ N268)
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
43% identity, 99% coverage: 3:393/396 of query aligns to 4:389/394 of 4fwrA
- active site: N8 (= N7), R83 (= R89), H172 (= H178), R233 (= R239), E378 (= E382)
- binding cytidine-5'-monophosphate: G202 (= G208), N203 (= N209), D275 (= D280), L276 (= L281), R277 (= R282), G323 (= G328), I324 (= I329), N327 (= N332)
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
43% identity, 99% coverage: 3:393/396 of query aligns to 4:389/394 of 4fwqA
- active site: N8 (= N7), R83 (= R89), H172 (= H178), R233 (= R239), E378 (= E382)
- binding guanosine-5'-triphosphate: H172 (= H178), N203 (= N209), G204 (= G210), D275 (= D280), L276 (= L281), R277 (= R282), E280 (≠ H285), G323 (= G328), I324 (= I329), N327 (= N332)
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
43% identity, 99% coverage: 3:393/396 of query aligns to 4:389/394 of 4fwpA
- active site: N8 (= N7), R83 (= R89), H172 (= H178), R233 (= R239), E378 (= E382)
- binding 1,2-ethanediol: S11 (= S10), H115 (= H121), K262 (≠ N268)
- binding guanosine-5'-diphosphate: N203 (= N209), D275 (= D280), L276 (= L281), R277 (= R282), E280 (≠ H285), G323 (= G328), I324 (= I329), N327 (= N332), S328 (≠ D333)
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
43% identity, 99% coverage: 3:393/396 of query aligns to 4:389/394 of 4fwoA