SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_027722494.1 NCBI__GCF_000425265.1:WP_027722494.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
57% identity, 98% coverage: 1:302/307 of query aligns to 2:303/306 of 4whxA

query
sites
4whxA

M

M

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active site: F37 (= F36), K160 (= K159), E194 (= E193), L217 (= L216)
binding alanine: Y97 (= Y96), K160 (= K159), A258 (= A257)

2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
52% identity, 98% coverage: 4:304/307 of query aligns to 3:303/305 of 2ej0B

query
sites
2ej0B

K

K

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active site: F35 (= F36), G37 (= G38), K158 (= K159), E192 (= E193), L215 (= L216)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y164), E192 (= E193), G195 (= G196), E196 (= E197), L215 (= L216), G217 (= G218), I218 (= I219), T219 (= T220), G254 (= G255), T255 (= T256)

1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
48% identity, 98% coverage: 4:303/307 of query aligns to 1:301/304 of 1iyeA

query
sites
1iyeA

K

K

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Y

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active site: F33 (= F36), G35 (= G38), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (= Y129), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)

1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
48% identity, 98% coverage: 4:303/307 of query aligns to 1:301/304 of 1iydA

query
sites
1iydA

K

K

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A

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Y

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E

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Y

A

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S

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H

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K

G

G

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A

V

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F

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N

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N

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M

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P

T

T

K

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A

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K

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C

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G

G

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E

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N

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F

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V

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K

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D

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G

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K

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P

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L

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Y

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A

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E

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F

F

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A

A

A

E

E

V

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P

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C

R

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V

V

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D

N

G

R

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G

A

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K

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V

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L

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Q

K

Q

E

A

Y

F

F

F

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A

L

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F

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T

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G

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P

E

K

D

Y

K

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W

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W

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L

L

E

D

active site: F33 (= F36), G35 (= G38), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
binding glutaric acid: Y92 (= Y96), Y126 (= Y129), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)

1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
48% identity, 98% coverage: 4:303/307 of query aligns to 1:301/304 of 1i1mA

query
sites
1i1mA

K

K

A

A

E

D

K

Y

I

I

W

W

F

F

D

N

G

G

E

E

L

M

V

V

D

R

W

W

D

E

A

D

A

A

Q

K

V

V

H

H

V

V

L

M

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

A

T

G

S

V

V

F

F

E

E

G

G

I

I

R

R

A

C

Y

Y

A

D

T

S

A

H

D

K

G

G

K

P

S

-

A

V

V

V

F

F

R

R

L

H

R

R

E

E

H

H

V

M

V

Q

R
|
R

L

L

F

H

D

D

S

S

A

A

K

K

I

I

L

Y

G

R

I

F

T

P

I

V

P

S

F

Q

S

S

V

I

E

D

E

E

I

L

H

M

N

E

A

A

I

C

L

R

E

D

T

V

L

I

K

R

V

K

N

N

G

N

L

L

K

T

E

S

G

A

Y
|
Y

I

I

R

R

P

P

L

L

V

I

F

F

I

V

G

G

D

D

G

V

A

G

M

M

G

G

V

V

H

N

P

P

G

P

S

A

N

G

-

Y

P

S

I

T

R

D

V

V

C

I

I

I

A

A

T

A

W

F

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

E

A

D

E

A

A

L

L

E

E

K

Q

G

G

I

I

R

D

V

A

K

M

T

V

S

S

F

W

N

N

R

R

H

A

V

P

N

N

A

T

M

I

M

P

T

T

K

A

S

A

K
|
K

A

A

C

G

G

G

N

N

Y
|
Y

V

L

N

S

S

S

I

L

L

L

A

V

K

G

V

S

E

E

A

A

V

R

K

R

D

H

G

G

Y

Y

D

Q

E

E

A

G

L

I

M

A

L

L

D

D

T

V

Q

N

G

G

Y

Y

V

I

S

S

E
|
E

A

G

T

A

G
|
G

E
|
E

N

N

I

L

F

F

I

E

V

V

K

K

N

D

G

G

I

V

I

L

K

F

T

T

P

P

L

F

T

T

-

S

S

S

V

A

L
|
L

P

P

G
|
G

I
|
I

T
|
T

R

R

A

D

S

A

L

I

M

I

K

K

V

L

A

A

R

K

D

E

L

L

G

G

Y

I

E

E

V

V

E

R

E

E

Q

Q

L

V

F

L

T

S

R

R

D

E

E

S

L

L

Y

Y

I

L

A

A

D

D

E

E

A

V

F

F

F

M

C

S

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

V

C

R

E

S

V

V

D

D

N

G

R

I

T

Q

I

V

G

G

A

E

G

G

K

R

R

C

G

G

E

P

V

V

G

T

T

K

L

R

L

I

Q

Q

K

Q

E

A

Y

F

F

F

N

G

A

L

V

F

K

T

G

G

G

E

N

T

P

E

K

D

Y

K

V

W

D

G

W

W

L

L

E

D

active site: K156 (= K159)
binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K159), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256)

1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
48% identity, 98% coverage: 4:303/307 of query aligns to 1:301/304 of 1i1lA

query
sites
1i1lA

K

K

A

A

E

D

K

Y

I

I

W

W

F

F

D

N

G

G

E

E

L

M

V

V

D

R

W

W

D

E

A

D

A

A

Q

K

V

V

H

H

V

V

L

M

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

A

T

G

S

V

V

F

F

E

E

G

G

I

I

R

R

A

C

Y

Y

A

D

T

S

A

H

D

K

G

G

K

P

S

-

A

V

V

V

F

F

R

R

L

H

R

R

E

E

H

H

V

M

V

Q

R
|
R

L

L

F

H

D

D

S

S

A

A

K

K

I

I

L

Y

G

R

I

F

T

P

I

V

P

S

F

Q

S

S

V

I

E

D

E

E

I

L

H

M

N

E

A

A

I

C

L

R

E

D

T

V

L

I

K

R

V

K

N

N

G

N

L

L

K

T

E

S

G

A

Y
|
Y

I

I

R

R

P

P

L

L

V

I

F

F

I

V

G

G

D

D

G

V

A

G

M

M

G

G

V

V

H

N

P

P

G

P

S

A

N

G

-

Y

P

S

I

T

R

D

V

V

C

I

I

I

A

A

T

A

W

F

P

P

W

W

G

G

A

A

Y

Y

L

L

G

G

E

A

D

E

A

A

L

L

E

E

K

Q

G

G

I

I

R

D

V

A

K

M

T

V

S

S

F

W

N

N

R

R

H

A

V

P

N

N

A

T

M

I

M

P

T

T

K

A

S

A

K
|
K

A

A

C

G

G

G

N

N

Y
|
Y

V

L

N

S

S

S

I

L

L

L

A

V

K

G

V

S

E

E

A

A

V

R

K

R

D

H

G

G

Y

Y

D

Q

E

E

A

G

L

I

M

A

L

L

D

D

T

V

Q

N

G

G

Y

Y

V

I

S

S

E
|
E

A

G

T

A

G
|
G

E

E

N

N

I

L

F

F

I

E

V

V

K

K

N

D

G

G

I

V

I

L

K

F

T

T

P

P

L

F

T

T

-

S

S

S

V

A

L

L

P

P

G
|
G

I
|
I

T
|
T

R

R

A

D

S

A

L

I

M

I

K

K

V

L

A

A

R

K

D

E

L

L

G

G

Y

I

E

E

V

V

E

R

E

E

Q

Q

L

V

F

L

T

S

R

R

D

E

E

S

L

L

Y

Y

I

L

A

A

D

D

E

E

A

V

F

F

F

M

C

S

G

G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

V

C

R

E

S

V

V

D

D

N

G

R

I

T

Q

I

V

G

G

A

E

G

G

K

R

R

C

G

G

E

P

V

V

G

T

T

K

L

R

L

I

Q

Q

K

Q

E

A

Y

F

F

F

N

G

A

L

V

F

K

T

G

G

G

E

N

T

P

E

K

D

Y

K

V

W

D

G

W

W

L

L

E

D

active site: K156 (= K159)
binding 2-methylleucine: Y92 (= Y96), K156 (= K159), T254 (= T256), A255 (= A257)
binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)

2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
50% identity, 98% coverage: 4:304/307 of query aligns to 3:295/297 of 2ej3A

query
sites
2ej3A

K

K

A

A

E

G

K

L

I

I

W

W

F

M

D

N

G

G

E

A

L

F

V

V

D

P

W

Q

D

E

A

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

A

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

A

E

T

T

A

A

D

K

G

G

K

-

S

P

A

A

V

I

F

F

R

R

L

L

R

K

E

E

H

H

V

V

V

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

K

K

I

V

L

L

G

R

I

M

T

E

I

I

P

P

F

F

S

A

V

P

E

E

I

L

H

E

N

E

A

A

I

I

L

K

E

E

T

V

L

V

K

R

V

R

N

N

G

G

L

Y

K

R

E

S

G

C

Y

Y

I

I

R

R

P

P

L

L

V

A

F

W

I

M

G

G

D

A

G

K

A

A

M

L

G

G

V

V

H

N

P

P

-

L

G

P

S

N

N

N

P

P

I

A

R

E

V

V

C

M

I

V

A

A

T

A

W

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

E

-

D

-

A

-

L

V

E

R

K

K

G

G

I

A

R

R

V

L

K

I

T

T

S

S

F

W

N

A

R

R

H

F

H

P

V

A

N

N

A

V

M

M

M

P

T

G

K

K

S

A

K
|
K

A

V

C

G

G

G

N

N

Y
|
Y

V

V

N

N

S

S

I

A

L

L

A

A

K

K

V

M

E

E

A

A

V

V

K

A

D

A

G

G

Y

A

D

D

E

E

A

A

L

L

M

L

L

L

D

D

T

E

Q

E

G

G

Y

Y

V

V

S

A

E
|
E

A

G

T

S

G
|
G

E

E

N

N

I

L

F

F

I

F

V

V

K

R

N

D

G

G

I

V

I

I

K

Y

T

A

P

L

P

E

L

H

T

S

S

V

V

N

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

A

D

S

S

L

V

M

I

K

R

V

I

A

A

R

K

D

D

L

L

G

G

Y

Y

E

E

V

V

E

Q

E

V

Q

V

L

R

F

A

T

T

R

R

D

D

E

Q

L

L

Y

Y

I

M

A

A

D

D

E

E

A

V

F

F

F

M

C

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

I

V

C

S

E

M

V

I

D

D

N

W

R

R

T

P

I

I

G

G

A

K

G

G

K

T

R

A

G

G

E

P

V

V

G

A

T

L

L

R

L

L

Q

R

K

E

E

V

Y

Y

F

L

N

E

A

A

V

V

K

T

G

G

G

R

N

R

P

P

K

E

Y

Y

V

E

D

G

W

W

L

L

E

T

Y

Y

active site: F35 (= F36), G37 (= G38), K150 (= K159), E184 (= E193), L207 (= L216)
binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G196), G246 (= G255), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
50% identity, 98% coverage: 4:304/307 of query aligns to 3:295/297 of 2eiyA

query
sites
2eiyA

K

K

A

A

E

G

K

L

I

I

W

W

F

M

D

N

G

G

E

A

L

F

V

V

D

P

W

Q

D

E

A

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

A

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

A

E

T

T

A

A

D

K

G

G

K

-

S

P

A

A

V

I

F

F

R

R

L

L

R

K

E

E

H

H

V

V

V

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

K

K

I

V

L

L

G

R

I

M

T

E

I

I

P

P

F

F

S

A

V

P

E

E

I

L

H

E

N

E

A

A

I

I

L

K

E

E

T

V

L

V

K

R

V

R

N

N

G

G

L

Y

K

R

E

S

G

C

Y
|
Y

I

I

R

R

P

P

L

L

V

A

F

W

I

M

G

G

D

A

G

K

A

A

M

L

G

G

V

V

H

N

P

P

-

L

G

P

S

N

N

N

P

P

I

A

R

E

V

V

C

M

I

V

A

A

T

A

W

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

E

-

D

-

A

-

L

V

E

R

K

K

G

G

I

A

R

R

V

L

K

I

T

T

S

S

F

W

N

A

R

R

H

F

H

P

V

A

N

N

A

V

M

M

M

P

T

G

K

K

S

A

K
|
K

A

V

C

G

G

G

N

N

Y
|
Y

V

V

N

N

S

S

I

A

L

L

A

A

K

K

V

M

E

E

A

A

V

V

K

A

D

A

G

G

Y

A

D

D

E

E

A

A

L

L

M

L

L

L

D

D

T

E

Q

E

G

G

Y

Y

V

V

S

A

E
|
E

A

G

T

S

G
|
G

E
|
E

N

N

I

L

F

F

I

F

V

V

K

R

N

D

G

G

I

V

I

I

K

Y

T

A

P

L

P

E

L

H

T

S

S

V

V

N

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

A

D

S

S

L

V

M

I

K

R

V

I

A

A

R

K

D

D

L

L

G

G

Y

Y

E

E

V

V

E

Q

E

V

Q

V

L

R

F

A

T

T

R

R

D

D

E

Q

L

L

Y

Y

I

M

A

A

D

D

E

E

A

V

F

F

F

M

C

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

I

V

C

S

E

M

V

I

D

D

N

W

R

R

T

P

I

I

G

G

A

K

G

G

K

T

R

A

G

G

E

P

V

V

G

A

T

L

L

R

L

L

Q

R

K

E

E

V

Y

Y

F

L

N

E

A

A

V

V

K

T

G

G

G

R

N

R

P

P

K

E

Y

Y

V

E

D

G

W

W

L

L

E

T

Y

Y

active site: F35 (= F36), G37 (= G38), K150 (= K159), E184 (= E193), L207 (= L216)
binding 4-methyl valeric acid: F35 (= F36), Y94 (= Y96), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (= E197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
50% identity, 98% coverage: 4:304/307 of query aligns to 3:295/297 of 1wrvA

query
sites
1wrvA

K

K

A

A

E

G

K

L

I

I

W

W

F

M

D

N

G

G

E

A

L

F

V

V

D

P

W

Q

D

E

A

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

A

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

A

E

T

T

A

A

D

K

G

G

K

-

S

P

A

A

V

I

F

F

R

R

L

L

R

K

E

E

H

H

V

V

V

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

K

K

I

V

L

L

G

R

I

M

T

E

I

I

P

P

F

F

S

A

V

P

E

E

I

L

H

E

N

E

A

A

I

I

L

K

E

E

T

V

L

V

K

R

V

R

N

N

G

G

L

Y

K

R

E

S

G

C

Y

Y

I

I

R

R

P

P

L

L

V

A

F

W

I

M

G

G

D

A

G

K

A

A

M

L

G

G

V

V

H

N

P

P

-

L

G

P

S

N

N

N

P

P

I

A

R

E

V

V

C

M

I

V

A

A

T

A

W

W

P

E

W

W

G

-

A

-

Y

-

L

-

G

-

E

-

D

-

A

-

L

V

E

R

K

K

G

G

I

A

R

R

V

L

K

I

T

T

S

S

F

W

N

A

R

R

H

F

H

P

V

A

N

N

A

V

M

M

M

P

T

G

K

K

S

A

K
|
K

A

V

C

G

G

G

N

N

Y
|
Y

V

V

N

N

S

S

I

A

L

L

A

A

K

K

V

M

E

E

A

A

V

V

K

A

D

A

G

G

Y

A

D

D

E

E

A

A

L

L

M

L

L

L

D

D

T

E

Q

E

G

G

Y

Y

V

V

S

A

E
|
E

A

G

T

S

G
|
G

E

E

N

N

I

L

F

F

I

F

V

V

K

R

N

D

G

G

I

V

I

I

K

Y

T

A

P

L

P

E

L

H

T

S

S

V

V

N

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

A

D

S

S

L

V

M

I

K

R

V

I

A

A

R

K

D

D

L

L

G

G

Y

Y

E

E

V

V

E

Q

E

V

Q

V

L

R

F

A

T

T

R

R

D

D

E

Q

L

L

Y

Y

I

M

A

A

D

D

E

E

A

V

F

F

F

M

C

T

G

G

T
|
T

A

A

E

E

V

V

T

T

P

P

I

V

C

S

E

M

V

I

D

D

N

W

R

R

T

P

I

I

G

G

A

K

G

G

K

T

R

A

G

G

E

P

V

V

G

A

T

L

L

R

L

L

Q

R

K

E

E

V

Y

Y

F

L

N

E

A

A

V

V

K

T

G

G

G

R

N

R

P

P

K

E

Y

Y

V

E

D

G

W

W

L

L

E

T

Y

Y

active site: F35 (= F36), G37 (= G38), K150 (= K159), E184 (= E193), L207 (= L216)
binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), T247 (= T256)

2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
50% identity, 98% coverage: 4:304/307 of query aligns to 3:292/294 of 2ej2A

query
sites
2ej2A

K

K

A

A

E

G

K

L

I

I

W

W

F

M

D

N

G

G

E

A

L

F

V

V

D

P

W

Q

D

E

A

E

A

A

Q

K

V

T

H

S

V

V

L

L

T

S

H

H

T

A

L

L

H

H

Y

Y

G

G

A

T

G

S

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

A

E

T

T

A

A

D

K

G

G

K

-

S

P

A

A

V

I

F

F

R

R

L

L

R

K

E

E

H

H

V

V

V

K

R
|
R

L

F

F

Y

D

N

S

S

A

A

K

K

I

V

L

L

G

R

I

M

T

E

I

I

P

P

F

F

S

A

V

P

E

E

I

L

H

E

N

E

A

A

I

I

L

K

E

E

T

V

L

V

K

R

V

R

N

N

G

G

L

Y

K

R

E

S

G

C

Y
|
Y

I

I

R

R

P

P

L

L

V

A

F

W

I

M

G

G

D

A

G

K

A

A

M

L

G

G

V

V

H

N

P

P

-

L

G

P

S

N

N

N

P

P

I

A

R

E

V

V

C

M

I

V

A

A

T

A

W

W

P

E

W

W

G

G

A

A

Y

-

L

-

G

-

E

-

D

-

A

-

L

-

E

-

K

-

G

-

I

-

R

R

V

L

K

I

T

T

S

S

F

W

N

A

R

R

H

F

H

P

V

A

N

N

A

V

M

M

M

P

T

G

K

K

S

A

K
|
K

A

V

C

G

G

G

N

N

Y
|
Y

V

V

N

N

S

S

I

A

L

L

A

A

K

K

V

M

E

E

A

A

V

V

K

A

D

A

G

G

Y

A

D

D

E

E

A

A

L

L

M

L

L

L

D

D

T

E

Q

E

G

G

Y

Y

V

V

S

A

E
|
E

A

G

T

S

G
|
G

E
|
E

N

N

I

L

F

F

I

F

V

V

K

R

N

D

G

G

I

V

I

I

K

Y

T

A

P

L

P

E

L

H

T

S

S

V

V

N

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

A

D

S

S

L

V

M

I

K

R

V

I

A

A

R

K

D

D

L

L

G

G

Y

Y

E

E

V

V

E

Q

E

V

Q

V

L

R

F

A

T

T

R

R

D

D

E

Q

L

L

Y

Y

I

M

A

A

D

D

E

E

A

V

F

F

F

M

C

T

G

G

T
|
T

A
|
A

A

A

E

E

V

V

T

T

P

P

I

V

C

S

E

M

V

I

D

D

N

W

R

R

T

P

I

I

G

G

A

K

G

G

K

T

R

A

G

G

E

P

V

V

G

A

T

L

L

R

L

L

Q

R

K

E

E

V

Y

Y

F

L

N

E

A

A

V

V

K

T

G

G

G

R

N

R

P

P

K

E

Y

Y

V

E

D

G

W

W

L

L

E

T

Y

Y

active site: F35 (= F36), G37 (= G38), K147 (= K159), E181 (= E193), L204 (= L216)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y164), E181 (= E193), G184 (= G196), E185 (= E197), L204 (= L216), G206 (= G218), I207 (= I219), T208 (= T220), T244 (= T256), A245 (= A257)

6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
46% identity, 96% coverage: 7:302/307 of query aligns to 8:297/301 of 6thqB

query
sites
6thqB

K

K

I

V

W

W

F

L

D

D

G

G

E

R

L

L

V

V

D

D

W

E

D

E

A

E

A

A

Q

K

V

V

H

T

V

V

L

L

T

S

H

P

T

S

L

L

H

N

Y

Y

G

G

A

F

G

G

V

V

F
|
F

E

E

G

G

I

I

R

R

A

A

Y

Y

A

W

T

N

A

G

D

E

G

N

K

L

S

Y

A

-

V

V

F

F

R

R

L

L

R

R

E

D

H

H

V

M

V

E

R
|
R

L

L

F

L

D

R

S

S

A

A

K

K

I

I

L

I

G

G

I

L

T

D

I

V

P

P

F

Y

S

T

V

A

E

E

I

L

H

S

N

K

A

A

I

V

L

V

E

E

T

T

L

V

K

R

V

A

N

N

G

G

L

F

K

K

E

E

G

D

-

L

Y
|
Y

I

I

R

R

P

P

L

V

V

A

F

Y

I

I

G

S

D

K

G

P

A

Q

M

I

G

S

V

L

H

D

P

V

G

R

S

G

N

L

P

Q

I

A

R

S

V

V

C

A

I

I

A

A

T

A

W

I

P

P

W

F

G

G

A

K

Y

Y

L

L

G

K

E

V

D

E

A

-

L

-

E

-

K

-

G

G

I

V

R

R

V

A

K

A

T

V

S

V

S

S

F

W

N

R

R

R

H

V

H

H

V

T

N

S

A

M

M

M

M

P

T

V

K

M

S

A

K
|
K

A

A

C

T

G

G

N

I

Y
|
Y

V

L

N

N

S

S

I

I

L

M

A

A

K

A

V

V

E

E

A

A

V

R

K

A

D

R

G

G

Y

Y

D

D

E

E

A

A

L

I

M

M

L

L

D

N

T

A

Q

E

G

G

Y

K

V

V

S

V

E
|
E

A

G

T

S

G
|
G

E
|
E

N
|
N

I

I

F

F

I

I

V

V

K

R

N

R

G

G

I

V

I

L

K

M

T

T

P

P

L

L

T

E

S

D

-

G

V

I

L
|
L

P

E

G
|
G

I
|
I

T
|
T

R

R

A

E

S

T

L

V

M

I

K

S

V

I

A

A

R

G

D

D

L

L

G

G

Y

I

E

P

V

L

E

L

E

E

Q

K

L

S

F

I

T

T

R

R

D

E

E

E

L

L

Y

Y

I

A

A

A

D

D

E

E

A

A

F

F

C

V

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

T

P

P

I

I

C

I

E

E

V

I

D

D

N

G

R

R

T

V

I

L

G

-

A

-

G

-

K

Q

R

R

G

G

E

P

V

I

G

T

T

Q

L

K

L

I

Q

A

K

E

E

T

Y

Y

F

R

N

R

A

I

V

V

K

L

G

G

G

K

N

E

P

E

K

K

Y

Y

V

L

D

P

W

W

L

L

active site: F37 (= F36), K156 (= K159), E190 (= E193), L214 (= L216)
binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), N195 (= N198), L214 (= L216), G216 (= G218), I217 (= I219), T218 (= T220), T254 (= T256)
binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), N195 (= N198), G216 (= G218), I217 (= I219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
45% identity, 91% coverage: 8:287/307 of query aligns to 4:277/290 of 5mr0D

query
sites
5mr0D

I

V

W

Y

F

M

D

D

G

G

E

E

L

F

V

V

D

P

W

E

D

N

A

E

A

A

Q

K

V

V

H

S

V

I

L

F

T

D

H

H

T

G

L

F

H

L

Y

Y

G

G

A

D

G

G

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

A

-

T

-

A

-

D

-

G

-

K

N

S

G

A

R

V

V

F

F

R

R

L

L

R

K

E

E

H

H

V

I

V

D

R
|
R

L

L

F

Y

D

D

S

S

A

A

K

K

I

A

L

I

G

D

I

L

T

E

I

I

P

P

F

I

S

T

V

K

E

E

I

F

H

M

N

E

A

I

I

I

L

L

E

E

T

T

L

L

K

R

V

K

N

N

G

N

L

L

K

R

E

D

G

A

Y

Y

I

I

R

R

P

P

L

I

V

V

F

T

I

R

G

G

D

I

G

G

A

D

M

L

G
|
G

V
x
L

H

D

P

P

G

R

-

K

-

C

S

Q

N

N

P

P

I

-

R

S

V

I

C

I

I

V

A

I

T

T

W

K

P

P

W

W

G

G

A

K

Y

L

L

Y

G

G

E

-

D

D

A

L

L

Y

E

E

K

K

G

G

I

L

R

T

V

A

K

I

T

T

S

V

S

A

F

V

N

R

R

R

H

N

H

S

V

F

N

D

A

A

M

L

M

P

T

P

K

N

S

I

K
|
K

A

S

C

L

G

-

N

N

Y
|
Y

V

L

N

N

S

N

I

I

L

L

A

A

K

K

V

I

E

E

A

A

V

N

K

A

D

K

G

G

Y

G

D

D

E

E

A

A

L

I

M

F

L

L

D

D

T

R

Q

N

G

G

Y

Y

V

V

S

S

E
|
E

A

G

T

S

G
|
G

E
x
D

N

N

I

I

F

F

I

V

V

V

K

K

N

N

G

G

I

A

I

I

K

T

T

T

P

P

L

T

T

I

S

N

V

N

L
|
L

P

R

G

G

I
|
I

T
|
T

R

R

A

E

S

A

L

V

M

I

K

E

V

I

A

I

R

N

D

R

L

L

G

G

Y

I

E

P

V

F

E

K

E

E

Q

T

L

N

F

I

T

G

R

L

D

Y

E

D

L

L

Y

Y

I

T

A

A

D

D

E

E

A

V

F

F

F

V

C

T

G
|
G

T
|
T

A

A

E

E

V

I

T

A

P

P

I

I

C

V

E

V

V

I

D

D

N

G

R

R

T

K

I

I

G

G

A

D

G

G

K

K

R

P

G

G

E

E

V

I

G

T

T

R

L

K

L

L

Q

M

K

E

E

E

Y

F

active site: F32 (= F36), G34 (= G38), K150 (= K159), E183 (= E193), L206 (= L216)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (= G109), L101 (≠ V110), K150 (= K159), Y154 (= Y164), E183 (= E193), G186 (= G196), D187 (≠ E197), L206 (= L216), I209 (= I219), T210 (= T220), G245 (= G255), T246 (= T256)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
45% identity, 91% coverage: 8:287/307 of query aligns to 5:278/290 of 5e25A

query
sites
5e25A

I

V

W

Y

F

M

D

N

G

G

E

E

L

F

V

V

D

P

W

E

D

S

A

Q

A

A

Q

K

V

V

H

S

V

V

L

F

T

D

H

H

T

G

L

F

H

L

Y

Y

G

G

A

D

G

G

V

V

F
|
F

E

E

G
|
G

I

I

R

R

A

A

Y

Y

A

-

T

-

A

-

D

N

G

G

K

K

S

-

A

-

V

V

F

F

R

K

L

L

R

Y

E

E

H

H

V

I

V

D

R
|
R

L

L

F

Y

D

D

S

C

A

A

K

R

I

V

L

I

G

D

I

L

T

K

I

I

P

P

F

L

S

S

V

K

E

E

I

F

H

A

N

E

A

A

I

I

L

L

E

E

T

T

L

L

K

R

V

R

N

N

G

N

L

L

K

R

E

D

G

A

Y
|
Y

I

I

R

R

P

P

L

I

V

V

F

T

I

R

G

G

D

A

G

G

A

D

M

L

G

G

V

L

H

D

P

P

G

R

S

K

N

C

P

P

I

S

-

P

R

N

V

V

C

I

I

I

A

I

T

T

W

K

P

P

W

W

G

G

A

K

Y

L

L

Y

G

G

E

-

D

D

A

L

L

Y

E

E

K

K

G

G

I

L

R

K

V

A

K

I

T

T

S

V

S

A

F

I

N

R

R

R

H

N

H

A

V

I

N

D

A

S

M

L

M

P

T

P

K

N

S

I

K
|
K

A

S

C

L

G

-

N

N

Y
|
Y

V

L

N

N

S

N

I

I

L

L

A

A

K

K

V

I

E

E

A

A

V

N

K

A

D

K

G

G

Y

G

D

D

E

E

A

A

L

I

M

F

L

L

D

D

T

H

Q

N

G

G

Y

Y

V

I

S

S

E
|
E

A

G

T

S

G
|
G

E
x
D

N

N

I

I

F

F

I

I

V

V

K

K

N

N

G

G

I

T

I

I

K

T

T

T

P

P

L

T

T

L

S

N

V

N

L
|
L

P

K

G
|
G

I
|
I

T
|
T

R

R

A

Q

S

V

L

V

M

I

K

E

V

L

A

I

R

N

D

E

L

L

G

E

Y

I

E

P

V

F

E

R

E

E

Q

A

L

N

F

I

T

G

R

L

D

F

E

D

L

L

Y

Y

I

S

A

A

D

D

E

E

A

I

F

F

F

V

C

T

G
|
G

T
|
T

A
|
A

A

A

E

E

V

I

T

A

P

P

I

V

C

T

E

Y

V

I

D

D

N

G

R

R

T

T

I

V

G

G

A

N

G

G

K

K

R

P

G

G

E

K

V

V

G

T

T

K

L

M

L

L

Q

M

K

E

E

K

Y

F

active site: F33 (= F36), G35 (= G38), K151 (= K159), E184 (= E193), L207 (= L216)
binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K159), T247 (= T256), A248 (= A257)
binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), D188 (≠ E197), L207 (= L216), G209 (= G218), I210 (= I219), T211 (= T220), G246 (= G255), T247 (= T256)

6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
36% identity, 98% coverage: 3:302/307 of query aligns to 1:301/307 of 6q8eA

query
sites
6q8eA

Q

E

K

N

A

P

E

K

K

Y

I

L

W

W

F

W

D

N

G

H

E

R

L

I

V

I

D

P

W

W

D

E

A

E

A

A

Q

T

V

V

H

H

V

L

L

T

T

D

H

Y

T

W

L

W

H

A

Y

S

G

V

A

T

G

A

V

V

F
|
F

E

E

G

G

I

I

R

R

A

G

Y

Y

-

W

A

N

T

N

A

A

D

E

G

G

K

E

S

M

A

Y

V

I

F

F

R

R

L

L

R

E

E

D

H

H

V

A

V

R

R
|
R

L

L

F

E

D

Q

S

S

A

M

K

Q

I

L

L

I

G

R

I

M

T

P

I

K

P

E

F

F

S

T

V

V

E

D

E

E

I

I

H

C

N

Q

A

A

I

T

L

I

E

D

T

L

L

V

K

R

V

A

N

N

G

D

L

Y

K

R

-

G

E

D

G

V

Y

Y

I

I

R

M

P

P

L

L

V

A

F

Y

-

A

I

V

G

G

D

N

G

K

A

A

M

F

G

S

V

V

H

-

P

V

G

G

S

D

N

R

P

T

I

T

R

E

V

M

C

F

I

I

A

Y

T

S

W

R

P

P

W

A

G

V

A

S

Y

R

L

L

G

E

E

E

D

D

A

F

L

-

E

-

K

-

G

S

I

L

R

H

V

A

K

C

T

Y

S

S

F

W

N

T

R

R

H

I

H

N

V

E

N

R

A

V

M

L

M

P

T

P

K

R

S

I

K
|
K

A

A

C

L

G

A

N

N

Y
|
Y

V

R

N

N

S

S

I

Q

L

L

A

A

K

S

V

S

E

E

A

A

V

A

K

M

D

N

G

G

Y

Y

D

D

E

T

A

A

L

L

M

F

L

L

D

N

T

P

Q

E

G

G

Y

K

V

V

S

A

E
|
E

A

G

T

T

G
|
G

E
x
S

N
x
C

I

V

F

F

I

F

V

V

K

R

N

K

G

G

I

K

I

L

K

I

T

T

P

P

P

D

L

I

T

T

S

S

-

G

V

I

L
|
L

P

E

G
x
S

I
|
I

T
|
T

R

R

A

D

S

T

L

V

M

I

K

H

V

L

A

A

R

R

D

E

-

V

L

L

G

G

Y

L

E

E

V

V

E

E

Q

R

L

V

F

V

T

D

R

R

D

T

E

E

L

T

Y

Y

I

L

A

A

D

D

E

E

A

A

F

F

F

L

C

C

G
|
G

T
|
T

A

H

A

A

E

E

V

I

T

T

P

P

I

I

C

A

E

S

V

I

D

D

N

R

R

H

T

E

I

M

G

K

A

H

G

G

K

A

R

P

G

G

E

P

V

I

G

T

T

R

L

Q

L

L

Q

R

K

D

E

I

Y

Y

F

R

N

E

A

V

V

V

K

Y

G

G

G

R

N

D

P

F

K

R

Y

Y

V

R

D

N

W

W

L

L

active site: F34 (= F36), K156 (= K159), E190 (= E193), L214 (= L216)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), S194 (≠ E197), C195 (≠ N198), L214 (= L216), S216 (≠ G218), I217 (= I219), T218 (= T220), G254 (= G255), T255 (= T256)

7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
35% identity, 98% coverage: 3:302/307 of query aligns to 1:301/309 of 7neaA

query
sites
7neaA

Q

E

K

N

A

P

E

K

K

Y

I

L

W

W

F

W

D

N

G

H

E

R

L

I

V

I

D

P

W

W

D

E

A

E

A

A

Q

T

V

V

H

H

V

L

L

T

T

D

H

Y

T

W

L

W

H

A

Y

S

G

V

A

T

G

A

V

V

F
|
F

E

E

G

V

I

I

R

S

A

G

Y

Y

-

W

A

N

T

N

A

A

D

E

G

G

K

E

S

M

A

Y

V

I

F

F

R

R

L

L

R

E

E

D

H

H

V

A

V

R

R
|
R

L

L

F

E

D

Q

S

S

A

M

K

Q

I

L

L

I

G

R

I

M

T

P

I

K

P

E

F

F

S

T

V

V

E

D

E

E

I

I

H

C

N

Q

A

A

I

T

L

I