SitesBLAST
Comparing WP_027722810.1 NCBI__GCF_000425265.1:WP_027722810.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 19 hits to proteins with known functional sites (download)
2c2bA Crystallographic structure of arabidopsis thaliana threonine synthase complexed with pyridoxal phosphate and s-adenosylmethionine (see paper)
33% identity, 86% coverage: 20:434/483 of query aligns to 16:416/444 of 2c2bA
- binding pyridoxal-5'-phosphate: F127 (= F131), K128 (= K132), D159 (= D164), G259 (≠ I267), G260 (= G268), N261 (= N269), L262 (≠ A270), G263 (= G271), N264 (= N272), A321 (= A332), H369 (≠ Q387), T397 (= T415)
- binding s-adenosylmethionine: S64 (≠ K70), T65 (≠ K71), W66 (≠ D72), P67 (≠ S73), G69 (vs. gap), S90 (≠ Y95), F92 (≠ G97), N97 (≠ P102), L98 (≠ I103), W100 (≠ A105), W115 (≠ Y120), W115 (≠ Y120), Q246 (≠ W253), F247 (≠ Y254)
Q9S7B5 Threonine synthase 1, chloroplastic; Protein METHIONINE OVER-ACCUMULATOR 2; EC 4.2.3.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
33% identity, 86% coverage: 20:434/483 of query aligns to 91:491/526 of Q9S7B5
- N172 (≠ P102) binding S-adenosyl-L-methionine
- L173 (≠ I103) binding S-adenosyl-L-methionine
- K181 (≠ N111) binding in monomer B; binding in monomer A
- N187 (≠ K117) binding in monomer B
- L205 (≠ R134) mutation to R: In mto2-1; causes a strong decrease in the concentration of soluble threonine and over-accumulation of methionine.
2c2gA Crystal structure of threonine synthase from arabidopsis thaliana in complex with its cofactor pyridoxal phosphate (see paper)
31% identity, 86% coverage: 20:434/483 of query aligns to 34:418/448 of 2c2gA
2zsjA Crystal structure of threonine synthase from aquifex aeolicus vf5
34% identity, 72% coverage: 75:420/483 of query aligns to 3:322/350 of 2zsjA
- active site: K61 (= K132), T85 (= T162), Q218 (= Q299), A222 (= A303), A240 (= A332), T317 (= T415)
- binding pyridoxal-5'-phosphate: F60 (= F131), K61 (= K132), N87 (≠ D164), V186 (≠ I267), G187 (= G268), N188 (= N269), A189 (= A270), G190 (= G271), N191 (= N272), A240 (= A332), T317 (= T415), G318 (≠ A416)
6nmxA Threonine synthase from bacillus subtilis atcc 6633 with plp and appa (see paper)
34% identity, 72% coverage: 73:420/483 of query aligns to 2:320/350 of 6nmxA
- active site: K60 (= K132), T84 (= T162), E216 (≠ Q299), S220 (≠ A303), A238 (= A332), T315 (= T415)
- binding (2E,3Z)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}-5-phosphonopent-3-enoic acid: K60 (= K132), S83 (= S161), T84 (= T162), N86 (≠ D164), T87 (= T165), F133 (= F213), N153 (= N233), S154 (= S234), R159 (= R239), V185 (≠ I267), G186 (= G268), N187 (= N269), A188 (= A270), G189 (= G271), N190 (= N272), A238 (= A332), I239 (≠ A333), E285 (≠ Q387), T315 (= T415)
6cgqB Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
34% identity, 72% coverage: 75:420/483 of query aligns to 2:318/345 of 6cgqB
- active site: K58 (= K132), T82 (= T162), E214 (≠ Q299), S218 (≠ A303), A236 (= A332), T313 (= T415)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: K58 (= K132), S81 (= S161), T82 (= T162), N84 (≠ D164), T85 (= T165), V183 (≠ I267), G184 (= G268), N185 (= N269), A186 (= A270), N188 (= N272), A236 (= A332), I237 (≠ A333), E283 (≠ Q387), T313 (= T415)
- binding phosphate ion: K58 (= K132), T85 (= T165), N151 (= N233), S152 (= S234), R157 (= R239), N185 (= N269)
6cgqA Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
33% identity, 71% coverage: 76:420/483 of query aligns to 1:310/339 of 6cgqA
- active site: K56 (= K132), T80 (= T162), E206 (≠ Q299), S210 (≠ A303), A228 (= A332), T305 (= T415)
- binding pyridoxal-5'-phosphate: F55 (= F131), K56 (= K132), N82 (≠ D164), V175 (≠ I267), G176 (= G268), N177 (= N269), A178 (= A270), G179 (= G271), N180 (= N272), A228 (= A332), E275 (≠ Q387), T305 (= T415), G306 (≠ A416)
2d1fA Structure of mycobacterium tuberculosis threonine synthase (see paper)
32% identity, 71% coverage: 76:420/483 of query aligns to 5:322/349 of 2d1fA
- active site: K60 (= K132), T84 (= T162), D209 (≠ T290), R213 (= R294), L215 (≠ F296), A240 (= A332), T317 (= T415)
- binding pyridoxal-5'-phosphate: F59 (= F131), K60 (= K132), N86 (≠ D164), V186 (≠ I267), G187 (= G268), N188 (= N269), A189 (= A270), G190 (= G271), N191 (= N272), A240 (= A332), T317 (= T415)
P9WG59 Threonine synthase; TS; EC 4.2.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
32% identity, 71% coverage: 76:420/483 of query aligns to 14:331/360 of P9WG59
- K69 (= K132) modified: N6-(pyridoxal phosphate)lysine
- N95 (≠ D164) binding pyridoxal 5'-phosphate
- K151 (≠ H222) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- GNAGN 196:200 (= GNAGN 268:272) binding pyridoxal 5'-phosphate
- T326 (= T415) binding pyridoxal 5'-phosphate
A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
31% identity, 71% coverage: 76:420/483 of query aligns to 14:331/360 of A0R220
- K151 (≠ H222) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
1uimA Crystal structure of threonine synthase from thermus thermophilus hb8, orthorhombic crystal form (see paper)
33% identity, 71% coverage: 77:420/483 of query aligns to 6:322/350 of 1uimA
- active site: K61 (= K132), T85 (= T162), P212 (= P293), G216 (= G297), Q218 (= Q299), A240 (= A332), T317 (= T415)
- binding pyridoxal-5'-phosphate: F60 (= F131), K61 (= K132), N87 (≠ D164), G187 (= G268), N188 (= N269), A189 (= A270), G190 (= G271), N191 (= N272), A240 (= A332), E287 (≠ C385), T317 (= T415), G318 (≠ A416)
3aeyA Apo form of threonine synthase from thermus thermophilus hb8 (see paper)
33% identity, 71% coverage: 77:420/483 of query aligns to 5:321/350 of 3aeyA
- active site: K60 (= K132), T84 (= T162), P211 (= P293), G215 (= G297), Q217 (= Q299), A239 (= A332), T316 (= T415)
- binding sulfate ion: K60 (= K132), K60 (= K132), G85 (= G163), N86 (≠ D164), T87 (= T165), T87 (= T165), S154 (= S234), R159 (= R239), N187 (= N269), R228 (≠ Y310), V230 (= V312), E231 (≠ D313), R232 (≠ D314), A239 (= A332)
3aexA Catalytic intermediate analogue of threonine synthase from thermus thermophilus hb8 (see paper)
33% identity, 71% coverage: 77:420/483 of query aligns to 6:322/351 of 3aexA
- active site: K61 (= K132), T85 (= T162), P212 (= P293), G216 (= G297), Q218 (= Q299), A240 (= A332), T317 (= T415)
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: K61 (= K132), S84 (= S161), T85 (= T162), N87 (≠ D164), T88 (= T165), V186 (≠ I267), G187 (= G268), N188 (= N269), A189 (= A270), G190 (= G271), N191 (= N272), A240 (= A332), I241 (≠ A333), E287 (≠ C385), T317 (= T415)
- binding phosphate ion: K61 (= K132), T88 (= T165), N154 (= N233), S155 (= S234), R160 (= R239), N188 (= N269)
1v7cA Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue (see paper)
33% identity, 71% coverage: 77:420/483 of query aligns to 6:322/351 of 1v7cA
- active site: K61 (= K132), T85 (= T162), P212 (= P293), G216 (= G297), Q218 (= Q299), A240 (= A332), T317 (= T415)
- binding (2e)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-5-phosphonopent-2-enoic acid: K61 (= K132), S84 (= S161), T85 (= T162), N87 (≠ D164), T88 (= T165), F134 (= F213), N154 (= N233), S155 (= S234), R160 (= R239), V186 (≠ I267), G187 (= G268), N188 (= N269), A189 (= A270), G190 (= G271), N191 (= N272), A240 (= A332), I241 (≠ A333), E287 (≠ C385), T317 (= T415)
1vb3A Crystal structure of threonine synthase from escherichia coli
30% identity, 47% coverage: 125:350/483 of query aligns to 100:318/428 of 1vb3A
1kl7A Crystal structure of threonine synthase from yeast (see paper)
30% identity, 31% coverage: 127:275/483 of query aligns to 118:281/509 of 1kl7A
Sites not aligning to the query:
P16120 Threonine synthase; TS; EC 4.2.3.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
30% identity, 31% coverage: 127:275/483 of query aligns to 119:284/514 of P16120
- G277 (= G268) binding pyridoxal 5'-phosphate
- N278 (= N269) binding pyridoxal 5'-phosphate
- F279 (≠ A270) binding pyridoxal 5'-phosphate
- D281 (≠ N272) binding pyridoxal 5'-phosphate
Sites not aligning to the query:
- 449 binding pyridoxal 5'-phosphate
4f4fA X-ray crystal structure of plp bound threonine synthase from brucella melitensis
29% identity, 31% coverage: 125:275/483 of query aligns to 106:263/464 of 4f4fA
Sites not aligning to the query:
Q42598 Threonine synthase; TS; EC 4.2.3.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 31% coverage: 125:275/483 of query aligns to 110:277/514 of Q42598
Sites not aligning to the query:
- 319 modified: Phosphoserine
- 321 modified: Phosphoserine
Query Sequence
>WP_027722810.1 NCBI__GCF_000425265.1:WP_027722810.1
MTLANKFPEYRAKMEYHCLGCGKKYDIDELLYTCPECGSVFILEDLTFAELKKTSGAEWR
NIFDARCATKKDSLRGIFRFYELFAAVMEEDEILYLGEGNTPIVASSPALNEVTGIKTAY
KNDGQNPSASFKDRGMACAFSYINALLNKHDWDEILTVCASTGDTSAAAALYASYMDSRV
KSVVILPQGKVTPQQLAQPLGSGAKVLEVPGVFDDCMKVVEHLADNYRVALLNSKNAWRI
LGQESYAFEVAQWYDWDLKDKCIFVPIGNAGNITAIMAGFLKLYELEIITDLPRVFGVQS
AHADPVYRYYSVDDPKEREFKPVKVQPSVAQAAMIGNPVSFPRVKHFAEKFETIGGKKAF
QVIQVSEQQIMDSMLLANANGHIACTQGGECFAGLIRAKELGLISETESAVLDSTAHQLK
FVGFQDMYYQNNFPAEYEVTPDTSRSNKPELVINGTEKDSMPEAEYIAKAANNVVSMLGL
EKK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory