Comparing WP_027722883.1 NCBI__GCF_000425265.1:WP_027722883.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P23533 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Staphylococcus carnosus (strain TM300) (see paper)
42% identity, 67% coverage: 266:817/823 of query aligns to 3:562/573 of P23533
2wqdA Crystal structure of enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system in the dephosphorylated state (see paper)
40% identity, 67% coverage: 266:817/823 of query aligns to 2:562/570 of 2wqdA
2hwgA Structure of phosphorylated enzyme i of the phosphoenolpyruvate:sugar phosphotransferase system (see paper)
41% identity, 66% coverage: 268:810/823 of query aligns to 1:553/572 of 2hwgA
P08839 Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system, enzyme I; EC 2.7.3.9 from Escherichia coli (strain K12) (see 2 papers)
41% identity, 66% coverage: 268:810/823 of query aligns to 2:554/575 of P08839
2xz7A Crystal structure of the phosphoenolpyruvate-binding domain of enzyme i in complex with phosphoenolpyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
47% identity, 36% coverage: 522:820/823 of query aligns to 12:316/324 of 2xz7A
2xz9A Crystal structure from the phosphoenolpyruvate-binding domain of enzyme i in complex with pyruvate from the thermoanaerobacter tengcongensis pep-sugar phosphotransferase system (pts) (see paper)
47% identity, 36% coverage: 522:820/823 of query aligns to 5:309/317 of 2xz9A
P37349 PEP-dependent dihydroxyacetone kinase, phosphoryl donor subunit DhaM; Dihydroxyacetone kinase subunit M; EC 2.7.1.121 from Escherichia coli (strain K12) (see paper)
34% identity, 38% coverage: 161:474/823 of query aligns to 157:453/472 of P37349
Sites not aligning to the query:
1j6tA Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
45% identity, 17% coverage: 1:141/823 of query aligns to 1:143/144 of 1j6tA
P00550 PTS system mannitol-specific EIICBA component; EIICBA-Mtl; EII-Mtl; EC 2.7.1.197 from Escherichia coli (strain K12) (see 2 papers)
45% identity, 17% coverage: 1:141/823 of query aligns to 493:635/637 of P00550
Sites not aligning to the query:
5lu4A C4-type pyruvate phosphate dikinase: conformational intermediate of central domain in the swiveling mechanism (see paper)
27% identity, 44% coverage: 416:780/823 of query aligns to 423:834/850 of 5lu4A
Sites not aligning to the query:
Q02KR1 Phosphoenolpyruvate synthase; PEP synthase; Pyruvate, water dikinase; EC 2.7.9.2 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
27% identity, 47% coverage: 393:781/823 of query aligns to 360:772/791 of Q02KR1
5jvjB C4-type pyruvate phosphate dikinase: different conformational states of the nucleotide binding domain in the dimer (see paper)
26% identity, 44% coverage: 416:780/823 of query aligns to 350:779/797 of 5jvjB
Sites not aligning to the query:
5jvlA C4-type pyruvate phospate dikinase: nucleotide binding domain with bound atp analogue (see paper)
26% identity, 44% coverage: 416:780/823 of query aligns to 423:856/874 of 5jvlA
Sites not aligning to the query:
Q39735 Pyruvate, phosphate dikinase, chloroplastic; Cold-sensitive pyruvate, orthophosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Flaveria bidentis (Coastal plain yellowtops) (Ethulia bidentis) (see 2 papers)
26% identity, 44% coverage: 416:780/823 of query aligns to 502:935/953 of Q39735
Sites not aligning to the query:
P22983 Pyruvate, phosphate dikinase; Pyruvate, orthophosphate dikinase; EC 2.7.9.1 from Clostridium symbiosum (Bacteroides symbiosus) (see 4 papers)
27% identity, 44% coverage: 418:780/823 of query aligns to 424:853/874 of P22983
Sites not aligning to the query:
1kc7A Pyruvate phosphate dikinase with bound mg-phosphonopyruvate (see paper)
27% identity, 44% coverage: 418:780/823 of query aligns to 423:852/872 of 1kc7A
Sites not aligning to the query:
O23404 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 44% coverage: 416:780/823 of query aligns to 512:944/963 of O23404
1vbgA Pyruvate phosphate dikinase from maize (see paper)
26% identity, 44% coverage: 416:780/823 of query aligns to 423:856/874 of 1vbgA
Sites not aligning to the query:
C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component from Bacillus subtilis (strain 168) (see paper)
36% identity, 17% coverage: 1:140/823 of query aligns to 1:140/143 of C0H3V2
P11155 Pyruvate, phosphate dikinase 1, chloroplastic; Pyruvate, orthophosphate dikinase 1; EC 2.7.9.1 from Zea mays (Maize) (see 7 papers)
26% identity, 44% coverage: 416:780/823 of query aligns to 496:929/947 of P11155
Sites not aligning to the query:
>WP_027722883.1 NCBI__GCF_000425265.1:WP_027722883.1
MVNLNENNVNLGAKASGKLEAIEQVGKILVREGFIEPEYIDSMKRREAVANTFLGNGISI
PHGLPENRDKILKTGVAILQVPEGVIWNPGETVHIVVGIAARSDEHIEILTNLTHVLDDE
QTTSRLAITKDPAEIVRVLSGESRSVSRPAPTLDVSDFDVSIDVTIMGEHGLHARPATFF
VDIAKKYDSEIQVEFDGRSGNGKSLASLLKLGISGGKTMRIHAKGDDASLALATLKEAVD
EGLGEESEEAAIPRIEHGWRPQDVKQTIPGCTASPGLATGPVHQYTHSRIVVEAIAKDPK
HESSELAHAIAAARLNLRHLYNEVRAKSGEPRAAIFRAHEAFLDDPEILVETEALIRNGK
SAGYAWRQVIDDRVHILEQHDDELLAARAMDLRDVGRRVLRHLAGVVQDQPFTPSRPVIL
LAEDLTPSDTAQLDPSLILGFCTSGGGPTSHSAIIARSLGIPAIVAAGPSILEIVDDTIA
IIDGDSGNLYLEPSPSDIETADAAKLELEELRNEEYRARYEPALTTDGERIEVVANIGRV
SEAENAVNAGGEGVGLMRTEFLFLERDTPPDEEEQFQSYKTMVEALNGLPIIIRTLDIGG
DKAVAYLDLPPEDNPFLGERGIRLCLNRPEFFLVQLRAIFRASKYGPIRIMFPMIATLDE
LEAAKRLAEKARIEVGAEALEIGIMVEVPSVVSMAREFAQVVDFFSIGTNDLTQYVMAID
RVHPTLAAKADSLHPAVLRMIDQVVKASDEAGIWTGVCGGLAGEPLGATILAGLGVKELS
MVVPSIAAVKARIRSISMKQARELAQKALSCRDNKQVRALSLS
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory