SitesBLAST
Comparing WP_028310855.1 NCBI__GCF_000482785.1:WP_028310855.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
32% identity, 92% coverage: 19:261/263 of query aligns to 17:259/259 of 5zaiC
- active site: A65 (≠ H67), F70 (≠ M72), S82 (≠ F85), R86 (≠ S89), G110 (≠ A113), E113 (≠ Q116), P132 (≠ S135), E133 (≠ G136), I138 (≠ L141), P140 (≠ C143), G141 (≠ A144), A226 (= A228), F236 (≠ M238)
- binding coenzyme a: K24 (≠ R26), L25 (≠ F27), A63 (= A65), G64 (= G66), A65 (≠ H67), D66 (= D68), I67 (≠ L69), P132 (≠ S135), R166 (≠ F168), F248 (= F250), K251 (= K253)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
34% identity, 97% coverage: 7:261/263 of query aligns to 4:257/257 of 6slbAAA
- active site: Q64 (≠ H67), F69 (≠ M72), L80 (= L84), N84 (≠ S89), A108 (= A113), S111 (≠ Q116), A130 (≠ S135), F131 (≠ G136), L136 (= L141), P138 (≠ C143), D139 (≠ A144), A224 (= A228), G234 (≠ M238)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ K61), A62 (= A65), Q64 (≠ H67), D65 (= D68), L66 (= L69), Y76 (= Y80), A108 (= A113), F131 (≠ G136), D139 (≠ A144)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
33% identity, 97% coverage: 7:261/263 of query aligns to 6:256/256 of 3h81A
- active site: A64 (≠ H67), M69 (= M72), T79 (≠ R82), F83 (≠ A86), G107 (≠ A113), E110 (≠ Q116), P129 (≠ S135), E130 (≠ G136), V135 (≠ L141), P137 (≠ C143), G138 (≠ A144), L223 (≠ A228), F233 (≠ M238)
- binding calcium ion: F233 (≠ M238), Q238 (≠ A243)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
32% identity, 98% coverage: 1:258/263 of query aligns to 1:254/255 of 3q0jC
- active site: A65 (≠ H67), M70 (= M72), T80 (≠ R82), F84 (≠ A86), G108 (≠ A113), E111 (≠ Q116), P130 (≠ S135), E131 (≠ G136), V136 (≠ L141), P138 (≠ C143), G139 (≠ A144), L224 (≠ A228), F234 (≠ M238)
- binding acetoacetyl-coenzyme a: Q23 (≠ A25), A24 (≠ R26), L25 (≠ F27), A27 (= A29), A63 (= A65), G64 (= G66), A65 (≠ H67), D66 (= D68), I67 (≠ L69), K68 (≠ R70), M70 (= M72), F84 (≠ A86), G107 (≠ A112), G108 (≠ A113), E111 (≠ Q116), P130 (≠ S135), E131 (≠ G136), P138 (≠ C143), G139 (≠ A144), M140 (≠ T145)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
32% identity, 98% coverage: 1:258/263 of query aligns to 1:254/255 of 3q0gC
- active site: A65 (≠ H67), M70 (= M72), T80 (≠ R82), F84 (≠ A86), G108 (≠ A113), E111 (≠ Q116), P130 (≠ S135), E131 (≠ G136), V136 (≠ L141), P138 (≠ C143), G139 (≠ A144), L224 (≠ A228), F234 (≠ M238)
- binding coenzyme a: L25 (≠ F27), A63 (= A65), I67 (≠ L69), K68 (≠ R70), Y104 (≠ V109), P130 (≠ S135), E131 (≠ G136), L134 (≠ V139)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
33% identity, 97% coverage: 7:261/263 of query aligns to 1:245/245 of 6slaAAA
- active site: Q61 (≠ H67), L68 (≠ F85), N72 (≠ S89), A96 (= A113), S99 (≠ Q116), A118 (≠ S135), F119 (≠ G136), L124 (= L141), P126 (≠ C143), N127 (≠ A144), A212 (= A228), G222 (≠ M238)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ F27), A59 (= A65), Q61 (≠ H67), D62 (= D68), L63 (= L69), L68 (≠ F85), Y71 (≠ C88), A94 (≠ T111), G95 (≠ A112), A96 (= A113), F119 (≠ G136), I122 (≠ V139), L124 (= L141), N127 (≠ A144), F234 (= F250), K237 (= K253)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
33% identity, 97% coverage: 6:261/263 of query aligns to 8:266/266 of O53561
- K135 (≠ R131) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 131:138, 25% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ N138) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
33% identity, 96% coverage: 7:258/263 of query aligns to 6:249/250 of 3q0gD
- active site: A64 (≠ H67), M69 (= M72), T75 (≠ Y81), F79 (= F85), G103 (≠ A113), E106 (≠ Q116), P125 (≠ S135), E126 (≠ G136), V131 (≠ L141), P133 (≠ C143), G134 (≠ A144), L219 (≠ A228), F229 (≠ M238)
- binding Butyryl Coenzyme A: F225 (≠ M234), F241 (= F250)
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
33% identity, 98% coverage: 3:261/263 of query aligns to 2:261/261 of 5jbxB
- active site: A67 (≠ H67), R72 (= R73), L84 (≠ F85), R88 (≠ S89), G112 (≠ A113), E115 (≠ Q116), T134 (≠ S135), E135 (≠ G136), I140 (≠ L141), P142 (≠ C143), G143 (≠ A144), A228 (= A228), L238 (≠ M238)
- binding coenzyme a: S24 (≠ A25), R25 (= R26), R26 (≠ F27), A28 (= A29), A65 (= A65), D68 (= D68), L69 (= L69), K70 (≠ R70), L110 (≠ T111), G111 (≠ A112), T134 (≠ S135), E135 (≠ G136), L138 (≠ V139), R168 (≠ F168)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
33% identity, 93% coverage: 10:254/263 of query aligns to 11:253/260 of 2hw5C
- active site: A68 (≠ H67), M73 (= M72), S83 (≠ R82), L87 (≠ A86), G111 (≠ A113), E114 (≠ Q116), P133 (≠ S135), E134 (≠ G136), T139 (≠ L141), P141 (vs. gap), G142 (≠ C143), K227 (≠ A228), F237 (≠ M238)
- binding crotonyl coenzyme a: K26 (≠ A25), A27 (≠ R26), L28 (≠ F27), A30 (= A29), K62 (= K61), I70 (≠ L69), F109 (≠ T111)
6l3pA Crystal strcuture of feruloyl-coa hydratase lyase(fchl) complexed with coa
35% identity, 79% coverage: 15:221/263 of query aligns to 17:223/244 of 6l3pA
- active site: M69 (≠ H67), Y74 (≠ M72), R86 (= R87), Q90 (≠ V91), G114 (≠ A113), S117 (≠ Q116), S136 (= S135), E137 (≠ G136), I142 (≠ L141), P144 (≠ C143), G145 (≠ A144)
- binding coenzyme a: K28 (≠ R26), R29 (≠ F27), A31 (= A29), A67 (= A65), M69 (≠ H67), D70 (= D68), L71 (= L69), G113 (≠ A112)
Sites not aligning to the query:
Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
33% identity, 94% coverage: 14:260/263 of query aligns to 85:332/337 of Q8GYN9
Sites not aligning to the query:
- 20 H→V: Loss of peroxisomal targeting.
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
31% identity, 91% coverage: 19:258/263 of query aligns to 19:251/254 of 2dubA
- active site: A67 (≠ H67), M72 (= M72), S82 (≠ T90), G105 (≠ A113), E108 (≠ Q116), P127 (≠ S135), E128 (≠ G136), T133 (≠ L141), P135 (vs. gap), G136 (≠ C143), K221 (≠ A228), F231 (≠ M238)
- binding octanoyl-coenzyme a: K25 (≠ A25), A26 (≠ R26), L27 (≠ F27), A29 (= A29), A65 (= A65), A67 (≠ H67), D68 (= D68), I69 (≠ L69), K70 (≠ R70), G105 (≠ A113), E108 (≠ Q116), P127 (≠ S135), E128 (≠ G136), G136 (≠ C143), A137 (= A144)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
32% identity, 91% coverage: 19:258/263 of query aligns to 18:255/258 of 1ey3A
- active site: A66 (≠ H67), M71 (= M72), S81 (≠ R82), L85 (≠ A86), G109 (≠ A113), E112 (≠ Q116), P131 (≠ S135), E132 (≠ G136), T137 (≠ L141), P139 (vs. gap), G140 (≠ C143), K225 (≠ A228), F235 (≠ M238)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ A25), L26 (≠ F27), A28 (= A29), A64 (= A65), G65 (= G66), A66 (≠ H67), D67 (= D68), I68 (≠ L69), L85 (≠ A86), W88 (≠ M92), G109 (≠ A113), P131 (≠ S135), L135 (≠ V139), G140 (≠ C143)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
31% identity, 93% coverage: 10:254/263 of query aligns to 41:283/290 of P14604
- E144 (≠ Q116) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ G136) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
32% identity, 91% coverage: 19:258/263 of query aligns to 20:257/260 of 1dubA
- active site: A68 (≠ H67), M73 (= M72), S83 (≠ R82), L87 (≠ A86), G111 (≠ A113), E114 (≠ Q116), P133 (≠ S135), E134 (≠ G136), T139 (≠ L141), P141 (vs. gap), G142 (≠ C143), K227 (≠ A228), F237 (≠ M238)
- binding acetoacetyl-coenzyme a: K26 (≠ A25), A27 (≠ R26), L28 (≠ F27), A30 (= A29), A66 (= A65), A68 (≠ H67), D69 (= D68), I70 (≠ L69), Y107 (≠ V109), G110 (≠ A112), G111 (≠ A113), E114 (≠ Q116), P133 (≠ S135), E134 (≠ G136), L137 (≠ V139), G142 (≠ C143), F233 (≠ M234), F249 (= F250)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
32% identity, 91% coverage: 19:258/263 of query aligns to 20:255/258 of 1mj3A
- active site: A68 (≠ H67), M73 (= M72), S83 (≠ R82), L85 (= L84), G109 (≠ A113), E112 (≠ Q116), P131 (≠ S135), E132 (≠ G136), T137 (≠ L141), P139 (vs. gap), G140 (≠ C143), K225 (≠ A228), F235 (≠ M238)
- binding hexanoyl-coenzyme a: K26 (≠ A25), A27 (≠ R26), L28 (≠ F27), A30 (= A29), A66 (= A65), G67 (= G66), A68 (≠ H67), D69 (= D68), I70 (≠ L69), G109 (≠ A113), P131 (≠ S135), E132 (≠ G136), L135 (≠ V139), G140 (≠ C143)
2uzfA Crystal structure of staphylococcus aureus 1,4-dihydroxy-2-naphthoyl coa synthase (menb) in complex with acetoacetyl coa (see paper)
33% identity, 92% coverage: 14:256/263 of query aligns to 16:251/260 of 2uzfA
- active site: G70 (≠ H67), R80 (= R77), L84 (≠ S89), G108 (≠ A113), V111 (≠ Q116), T130 (≠ S135), G131 (= G136), S136 (≠ L141), D138 (≠ C143), A139 (= A144), A225 (= A230), Y233 (≠ M238)
- binding acetoacetyl-coenzyme a: V28 (≠ R26), R29 (≠ F27), S68 (≠ A65), G69 (= G66), G70 (≠ H67), D71 (= D68), Y104 (≠ V109), G108 (≠ A113)
4i52A Scmenb im complex with 1-hydroxy-2-naphthoyl-coa (see paper)
33% identity, 94% coverage: 15:260/263 of query aligns to 19:270/275 of 4i52A
- active site: G77 (≠ H67), R82 (= R73), Y87 (vs. gap), R95 (= R77), L99 (≠ S89), G123 (≠ A113), V126 (≠ Q116), G146 (= G136), S151 (≠ L141), D153 (vs. gap), G154 (≠ C143), A240 (= A230), Y248 (≠ M238)
- binding 1-hydroxy-2-naphthoyl-CoA: H29 (≠ A25), K30 (≠ R26), R31 (≠ F27), A33 (= A29), S75 (≠ A65), G76 (= G66), G77 (≠ H67), D78 (= D68), Q79 (≠ R70), L96 (= L78), V98 (≠ C88), Y119 (≠ V109), I121 (≠ T111), G123 (≠ A113), T145 (≠ S135), V149 (= V139), S151 (≠ L141), F152 (= F142)
4i4zA Synechocystis sp. Pcc 6803 1,4-dihydroxy-2-naphthoyl-coenzyme a synthase (menb) in complex with salicylyl-coa (see paper)
33% identity, 94% coverage: 15:260/263 of query aligns to 19:270/275 of 4i4zA
- active site: G77 (≠ H67), R82 (= R73), Y87 (vs. gap), R95 (= R77), L99 (≠ S89), G123 (≠ A113), V126 (≠ Q116), G146 (= G136), S151 (≠ L141), D153 (vs. gap), G154 (≠ C143), A240 (= A230), Y248 (≠ M238)
- binding Salicylyl CoA: H29 (≠ A25), K30 (≠ R26), R31 (≠ F27), S75 (≠ A65), G76 (= G66), G77 (≠ H67), D78 (= D68), Q79 (≠ R70), Y87 (vs. gap), V98 (≠ C88), G123 (≠ A113), T145 (≠ S135), V149 (= V139), S151 (≠ L141), F260 (= F250), K263 (= K253)
- binding bicarbonate ion: G122 (≠ A112), Q144 (≠ V134), T145 (≠ S135), G146 (= G136), W174 (≠ L163)
Query Sequence
>WP_028310855.1 NCBI__GCF_000482785.1:WP_028310855.1
MPPDQPLVLRDARDGVVTLTLNRPARFNALSEAMLDALGAALADTAADADARCVVLAGAG
KAFCAGHDLREMRGQPRLDYYRELFARCSTVMQAIRAVPVPVIARVHGVATAAGCQLVAA
CDLAIAGESARFAVSGINVGLFCATPSVALSRAVSAKRAFDMLVTGRFIDARTAAEWSLV
NEAVPDDQLDAAVADKAAVIRAKSPAAIRHGKALFHRQRELNLADAYALAGEVMAQNMME
ADACEGIDAFLEKRPPAWAKREG
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory