SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028311332.1 NCBI__GCF_000482785.1:WP_028311332.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
72% identity, 99% coverage: 4:279/280 of query aligns to 3:271/274 of 3tdtA

query
sites
3tdtA

E

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active site: D141 (= D149), G166 (= G174), E189 (= E197)
binding 2-amino-6-oxopimelic acid: R104 (= R112), M124 (= M132), M139 (= M147), D141 (= D149)
binding coenzyme a: G185 (= G193), A186 (= A194), S203 (= S211), M204 (= M212), R217 (= R225), V234 (= V242), K254 (= K262), K259 (≠ Q267), T260 (= T268), K263 (= K271)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
72% identity, 99% coverage: 4:279/280 of query aligns to 3:271/274 of 2tdtA

query
sites
2tdtA

E

Q

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L

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active site: D141 (= D149), G166 (= G174), E189 (= E197)
binding coenzyme a: S159 (= S167), F183 (= F191), G185 (= G193), S203 (= S211), M204 (= M212), R217 (= R225), K254 (= K262), K259 (≠ Q267), T260 (= T268), K263 (= K271)
binding (2S)-2-aminoheptanedioic acid: R104 (= R112), M124 (= M132), M139 (= M147), D141 (= D149)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
72% identity, 99% coverage: 4:279/280 of query aligns to 3:271/274 of 1kgtA

query
sites
1kgtA

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active site: D141 (= D149), G166 (= G174), E189 (= E197)
binding pimelic acid: R104 (= R112), M124 (= M132), M139 (= M147)
binding succinyl-coenzyme a: D141 (= D149), S159 (= S167), A186 (= A194), S203 (= S211), R217 (= R225), K259 (≠ Q267), T260 (= T268), K263 (= K271)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
72% identity, 99% coverage: 4:279/280 of query aligns to 3:271/274 of 1kgqA

query
sites
1kgqA

E

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L

L

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active site: D141 (= D149), G166 (= G174), E189 (= E197)
binding (2S)-2-aminoheptanedioic acid: R104 (= R112), M124 (= M132), M139 (= M147), D141 (= D149)
binding succinamide-coa: F183 (= F191), G185 (= G193), S203 (= S211), M204 (= M212), R217 (= R225), V234 (= V242), K254 (= K262), K259 (≠ Q267), T260 (= T268), K263 (= K271)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
39% identity, 35% coverage: 105:202/280 of query aligns to 70:168/203 of 3r8yA

query
sites
3r8yA

D

D

F

L

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K

A

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A

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H

H

L

V

S

G

G

A

G

G

V

A

G

V

I

L

G

A

G
|
G

V

V

L

I

E

E

P

P

L

P

Q

S

A

A

N

K

P

P

T

V

I

I

I

V

E

E

D

D

N

D

C

V

F

V

I

I

G

G

A

A

R

N

S

V

E
x
V

I

V

V

L

E

E

G

G

V

V

active site: D115 (= D149), G140 (= G174), V163 (≠ E197)
binding calcium ion: G121 (= G155), G122 (≠ S156)

3r5bA Pseudomonas aeruginosa dapd (pa3666) in complex with l-2-aminopimelate (see paper)
27% identity, 51% coverage: 79:222/280 of query aligns to 153:284/321 of 3r5bA

query
sites
3r5bA

A

A

G

R

L

L

D

K

G

G

A

K

L

L

A

L

P

E

I

V

Q

F

F

S

Y

V

D

D

K

K

V

F

P

P

T

-

K

-

F

-

A

-

G

-

F

-

S

K

A

M

A

T

D

D

F

Y

A

V

A

V

-

P

A

A

G

G

V

V

R

R

V

I

V

A

P

D

P

T

A

A

V

R

A

V

R
|
R

R

L

G

G

S

A

F

Y

I

I

A

G

K

E

N

G

V

T

V

T

L

V

M

M

-

H

P

E

S

G

Y

F

V

V

N

N

I

F

G

N

A

A

Y

G

V

T

D

E

S

G

G

P

T

G

M

M

V

I

D

E

T

G

W

R

A

V

T
x
S

V

A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G

M

G

G

V

T

L

L

E

N

P

I

L

V

Q

I

A

S

N

-

P

-

T

-

I

-

I

V

E

G

D

E

N

G

C

C

F

L

I

I

G

G

A

A

R

N

S

A

E

G

I

I

V

-

E

-

G

G

V

I

I

P

V

L

E

G

E

D

N

R

S

N

V

I

I

V

S

E

M

A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S

G

T

T

K

K

I

V

binding (2S)-2-aminoheptanedioic acid: R189 (= R120), S223 (≠ T153)

Sites not aligning to the query:

binding (2S)-2-aminoheptanedioic acid: 127

3r5aA Pseudomonas aeruginosa dapd (pa3666) in complex with d-2-aminopimelate (see paper)
27% identity, 51% coverage: 79:222/280 of query aligns to 153:284/321 of 3r5aA

query
sites
3r5aA

A

A

G

R

L

L

D

K

G

G

A

K

L

L

A

L

P

E

I

V

Q

F

F

S

Y

V

D

D

K

K

V

F

P

P

T

-

K

-

F

-

A

-

G

-

F

-

S

K

A

M

A

T

D

D

F

Y

A

V

A

V

-

P

A

A

G

G

V

V

R
|
R

V

I

V

A

P

D

P

T

A

A

V

R

A

V

R
|
R

R

L

G

G

S

A

F

Y

I

I

A

G

K

E

N

G

V

T

V

T

L

V

M
|
M

-

H

P

E

S

G

Y

F

V

V

N
|
N

I

F

G

N

A

A

Y

G

V

T

D

E

S

G

G

P

T

G

M
|
M

V

I

D

E

T

G

W

R

A

V

T
x
S

V
x
A

G

G

S

V

C

F

A

-

Q

-

I

V

G

G

K

K

N

G

V

S

H

D

L

L

S

G

G

G

G

G

V

C

G

S

I

T

G

M

G

G

V

T

L

L

E

N

P

I

L

V

Q

I

A

S

N

-

P

-

T

-

I

-

I

V

E

G

D

E

N

G

C

C

F

L

I

I

G

G

A

A

R

N

S

A

E

G

I

I

V

-

E

-

G

G

V

I

I

P

V

L

E

G

E

D

N

R

S

N

V

I

I

V

S

E

M

A

G

G

V

L

Y

Y

I

I

G

T

Q

A

S

G

T

T

K

K

I

V

binding (2R)-2-aminoheptanedioic acid: R181 (= R112), R189 (= R120), M201 (= M132), N207 (= N137), M217 (= M147), S223 (≠ T153), A224 (≠ V154)

Sites not aligning to the query:

binding (2R)-2-aminoheptanedioic acid: 127

Query Sequence

>WP_028311332.1 NCBI__GCF_000482785.1:WP_028311332.1
MTTELQSIIEQAWDNRAELSPTSAPAPIAEAVEHVLAELDSGKLRVAEKIDGDWTVHQWI
KKAVLLSFRLADNAPMPFAGLDGALAPIQFYDKVPTKFAGFSAADFAAAGVRVVPPAVAR
RGSFIAKNVVLMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQ
ANPTIIEDNCFIGARSEIVEGVIVEENSVISMGVYIGQSTKIYDRATGEVYYGRVPSGSV
VVPGSLPSADGKYSLYCAVIVKRVDAQTRAKTGINELLRD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory