SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028314150.1 NCBI__GCF_000429905.1:WP_028314150.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7d7oB Crystal structure of cystathionine gamma-lyase from bacillus cereus atcc 14579 (see paper)
40% identity, 100% coverage: 1:374/375 of query aligns to 1:376/377 of 7d7oB

query
sites
7d7oB

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binding pyridoxal-5'-phosphate: Y44 (= Y41), R46 (= R43), G74 (= G72), M75 (= M73), Y98 (= Y97), D170 (= D168), T172 (= T170), S192 (= S190), T194 (= T192), K195 (= K193)

5dx5A Crystal structure of methionine gamma-lyase from clostridium sporogenes (see paper)
38% identity, 99% coverage: 5:374/375 of query aligns to 10:394/399 of 5dx5A

query
sites
5dx5A

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active site: R59 (= R43), Y112 (= Y97), D186 (= D168), K211 (= K193)
binding pyridoxal-5'-phosphate: Y57 (= Y41), R59 (= R43), S86 (= S71), G87 (= G72), M88 (= M73), Y112 (= Y97), D186 (= D168), F189 (= F171), S208 (= S190), T210 (= T192), K211 (= K193)

4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
39% identity, 100% coverage: 1:374/375 of query aligns to 1:370/373 of 4l0oH

query
sites
4l0oH

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active site: R40 (= R43), Y92 (= Y97), D164 (= D168), K189 (= K193)
binding pyridoxal-5'-phosphate: Y38 (= Y41), R40 (= R43), S67 (= S71), G68 (= G72), L69 (≠ M73), Y92 (= Y97), D164 (= D168), S186 (= S190), T188 (= T192), K189 (= K193)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
38% identity, 99% coverage: 5:374/375 of query aligns to 10:381/386 of 3mkjA

query
sites
3mkjA

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L

S

L

A

D

S

D

A

L

I

Y
|
Y

G

G

G

C

T

T

H

H

S

A

M

F

A

L

T

S

A

H

D

S

F

M

E

P

R

K

L

F

G

G

L

I

E

N

Y

V

D

S

F

F

V

V

-

D

A

A

T

A

S

K

L

P

E

E

A

E

I

I

M

R

A

A

A

A

Q

M

K

R

P

P

N

E

T

T

T

K

M

V

V

V

I

Y

I

I

E
|
E

S

T

P

P

T

A

N

N

P

P

L

T

M

L

N

S

I

L

L

V

D

D

I

I

K

E

A

T

T

V

A

A

D

G

W

I

A

A

R

H

E

Q

N

Q

K

G

I

A

T

L

S

L

L

V

M

V

D
|
D

N

N

T

T

F
|
F

G

M

T

S

S

P

I

Y

N

C

Q

Q

T

Q

P

P

L

L

A

Q

L

L

G

G

I

A

D

D

I

I

V

V

V

V

H

H

S
|
S

G

V

T
|
T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

S

G

D

D

V

V

C

I

C

G

G

G

F

I

A

I

L

V

A

G

S

K

K

-

K

-

K

-

M

-

Q

Q

P

E

I

F

L

I

D

D

L

Q

A

A

R

R

H

F

L

V

G

G

-

L

-

K

-

D

-

I

-

T

-

G

G

G

S

C

L

M

D

S

S

P

F

F

A

N

A

A

Y

W

L

L

L

T

E

L

R

R

S

G

L

V

K

K

T

T

L

L

A

G

I

I

R

R

V

M

E

E

R

R

Q

H

C

C

Q

E

N

N

A

A

Q

L

A

K

L

I

A

A

E

R

F

F

L

L

E

E

A

G

H

H

P

P

K

S

I

I

V

T

K

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

P

S

N

S

F

H

P

P

G

Q

H

Y

D

E

I

L

A

G

K

Q

K

R

Q

Q

M

M

K

S

L

L

F

P

G

G

A

G

M

I

M

I

S

S

F

F

E

E

L

I

D

A

E

G

S

G

I

L

G

E

D

A

V

G

D

R

V

R

F

M

L

I

R

N

K

S

L

V

K

E

V

L

I

C

H

L

P

L

A

A

V

V

S

S

L

L

G

G

G

D

V

T

E

E

S

T

S

L

I

I

C

Q

S

H

P

P

K

A

D

S

T

M

S

T

H

H

K

S

P

P

M

V

T

A

A

P

E

E

E

E

R

R

A

L

R

K

V

A

G

G

I

I

S

T

D

D

F

G

L

L

L

I

R

R

L

L

S

S

I

V

G

G

I

L

E

E

H

D

I

P

D

E

D

D

L

I

K

I

E

N

D

D

I

L

E

E

Q

H

A

A

L

I

active site: Y101 (= Y97), D173 (= D168), K198 (= K193)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G72), I77 (≠ M73), Y101 (= Y97), E144 (= E139), D173 (= D168), F176 (= F171), S195 (= S190), T197 (= T192), K198 (= K193)

7ba4A Structure of cystathionine gamma-lyase from pseudomonas aeruginosa
39% identity, 99% coverage: 5:374/375 of query aligns to 9:371/377 of 7ba4A

query
sites
7ba4A

T

T

Q

R

C

V

V

I

Q

H

A

A

G

G

T

Q

L

A

K

P

D

D

N

P

Q

S

Y

T

Q

G

G

A

L

I

N

M

S

P

P

P

L

I

Y

Y

T

A

S

N

T

S

A

T

Y

Y

G

-

Y

-

M

I

D

Q

K

R

E

S

E

H

V

-

C

-

Y

-

P

-

R

-

Y

-

F

-

T

-

T

N

P

P

N

T

Q

R

G

W

A

A

V

L

V

E

K

R

K

C

I

V

C

A

A

D

L

L

E

E

G

G

A

G

E

T

D

Q

G

A

L

F

L

A

F

F

S

A

S

S

G
|
G

M
x
L

A

A

A

A

V

I

S

S

T

S

T

-

I

V

L

L

A

E

H

L

T

L

R

D

S

A

G

G

D

S

H

H

V

I

I

V

L

S

L

G

D

N

D

D

L

L

Y
|
Y

G

G

G

G

T

T

H

F

S

R

M

L

A

-

T

-

A

-

D

-

F

F

E

E

R

R

L

V

-

R

-

R

-

R

-

S

-

A

G

G

L

H

E

R

Y

F

D

S

F

F

V

V

-

D

A

P

T

T

S

D

L

L

E

Q

A

A

I

F

M

E

A

A

A

A

Q

L

K

T

P

P

N

E

T

T

T

R

M

M

V

V

I

W

I

V

E

E

S

T

P

P

T

S

N

N

P

P

L

L

M

L

N

R

I

L

L

T

D

D

I

L

K

R

A

A

T

I

A

A

D

Q

W

L

A

C

R

R

E

A

N

R

K

G

I

I

T

I

S

S

L

V

M

A

D
|
D

N

N

T

T

F
|
F

G

A

T

S

S

P

I

Y

N

I

Q

Q

T

R

P

P

L

L

A

E

L

L

G

G

I

F

D

D

I

V

V

V

V

V

H

H

S
|
S

G

T

T
|
T

K
|
K

Y

Y

M

L

G

N

G

G

H

H

S

S

D

D

V

V

C

I

C

G

G

G

F

I

A

A

L

I

A

V

S

G

K

D

K

N

K

P

M

-

Q

-

P

-

I

-

L

-

D

D

L

L

A

R

R

E

H

R

L

L

G

G

G

F

S

L

L

Q

D

N

S

S

-

V

-

G

-

A

-

I

-

S

-

G

-

P

F

F

A

D

A

A

Y

F

L

L

L

T

E

L

R

R

S

G

L

V

K

K

T

T

L

L

A

A

I

L

R

R

V

M

E

E

R

R

Q

H

C

C

Q

S

N

N

A

A

Q

L

A

A

L

L

A

A

E

Q

F

W

L

L

E

E

A

R

H

Q

P

P

K

Q

I

V

V

A

K

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

P

A

N

S

F

H

P

P

G

Q

H

H

D

E

I

L

A

A

K

K

K

R

Q

Q

M

M

K

R

L

G

F

F

G

G

A

G

M

M

M

I

S

S

F

L

E

D

L

L

D

R

E

C

S

D

I

L

G

A

D

G

V

A

D

R

V

R

F

F

L

L

R

E

K

N

L

V

K

R

V

I

I

F

H

S

P

L

A

A

V

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

S

L

I

I

C

E

S

H

P

P

K

A

D

I

T

M

S

T

H

H

K

A

P

S

M

I

T

P

A

A

E

E

E

T

R

R

A

A

R

D

V

L

G

G

I

I

S

G

D

D

F

S

L

L

L

I

R

R

L

L

S

S

I

V

G

G

I

V

E

E

H

A

I

L

D

E

D

D

L

L

K

Q

E

A

D

D

I

L

E

A

Q

Q

A

A

L

L

binding pyridoxal-5'-phosphate: G66 (= G72), L67 (≠ M73), Y90 (= Y97), D163 (= D168), F166 (= F171), S185 (= S190), T187 (= T192), K188 (= K193)

5x5hA Crystal structure of metb from corynebacterium glutamicum (see paper)
40% identity, 99% coverage: 5:374/375 of query aligns to 10:381/385 of 5x5hA

query
sites
5x5hA

T

T

Q

A

C

S

V

I

Q

H

A

A

G

G

T

Y

L

E

K

P

D

D

N

D

Q

Y

Y

Y

Q

G

G

S

L

I

N

N

S

T

P

P

L

I

Y

Y

T

A

S

S

T

T

A

T

Y
x
F

G

A

Y
x
Q

M

N

D

A

K

P

E

N

E

E

V

L

C

R

Y

K

P

G

R

Y

Y
x
E

F

Y

T

T

-
x
R

-

V

-

G

T

N

P

P

N

T

Q

I

G

V

A

A

V

L

V

E

K

Q

K

T

I

V

C

A

A

A

L

L

E
|
E

G

G

A

A

E

K

D

Y

G

G

L

R

L

A

F

F

S

S

S

S

G
|
G

M
|
M

A

A

A

A

V

T

S

D

T

I

T

L

I

F

L

R

A

I

H

I

T

L

R

K

S

P

G

G

D

D

H

H

V

I

I

V

L

L

L

G

D

N

D

D

L

A

Y
|
Y

G

G

G

G

T

T

H

Y

S

R

M

L

A

I

T

D

A

T

D

V

F

F

E

T

R

A

L

W

G

G

L

V

E

E

Y

Y

D

T

F

V

V

V

A

D

T

T

S

S

L

V

-

V

E

E

A

E

I

V

M

K

A

A

A

A

Q

I

K

K

P

D

N

N

T

T

T

K

M

L

V

I

I

W

I

V

E

E

S

T

P

P

T

T

N

N

P

P

L

A

M

L

N

G

I

I

L

T

D

D

I

I

K

E

A

A

T

V

A

A

D

K

W

L

A

T

R

E

E

G

N

T

K

N

I

A

T

K

S

L

L

V

M

V

D
|
D

N

N

T

T

F

F

G

A

T

S

S

P

I

Y

N

L

Q

Q

T
x
Q

P
|
P

L
|
L

A

K

L

L

G

G

I

A

D

H

I

A

V

V

V

L

H

H

S
|
S

G

T

T
|
T

K
|
K

Y

Y

M

I

G

G

G

G

H

H

S

S

D

D

V

V

C

V

C

G

G

G

F

L

A

V

L

V

A

T

S

N

K

D

K

Q

K

E

M

M

-

D

Q

E

P

E

I

L

L

L

D

F

L

M

A

Q

R

G

H

G

L

I

G

G

G

P

S

I

L

P

D

S

S

V

F

F

A

D

A

A

Y

Y

L

L

L

T

E

A

R

R

S

G

L

L

K

K

T

T

L

L

A

A

I

V

R

R

V

M

E

D

R

R

Q

H

C

C

Q

D

N

N

A

A

Q

E

A

K

L

I

A

A

E

E

F

F

L

L

E

D

A

S

H

R

P

P

K

E

I

V

V

S

K

T

V

V

N

L

Y

Y

P

P

G

G

L

L

P

K

N

N

F

H

P

P

G

G

H

H

D

E

I

V

A

A

K

A

K

K

Q

Q

M

M

K

K

L

R

F

F

G

G

A

G

M

M

M

I

S

S

F

V

E

R

L

F

D

A

E

G

S

G

I

E

G

E

D

A

V

A

D

K

V

K

F

F

L

C

R

T

K

S

L

T

K

K

V

L

I

I

H

C

P

L

A

A

V

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

S

L

I

L

C

E

S

H

P

P

K

A

D

T

T

M

S

T

H

H

K

Q

P

S

M

A

T

A

A

G

E

-

E

-

R

-

A

S

R

Q

V

L

G

E

I

V

S

P

D

R

F

D

L

L

L

V

R

R

L

I

S

S

I

I

G

G

I

I

E

E

H

D

I

I

D

E

D

D

L

L

K

L

E

A

D

D

I

V

E

E

Q

Q

A

A

L

L

active site: R52 (vs. gap), Y105 (= Y97), D177 (= D168), K202 (= K193)
binding magnesium ion: F36 (≠ Y31), Q38 (≠ Y33), E49 (≠ Y44), E69 (= E61), Q187 (≠ T178), P188 (= P179), L189 (= L180)
binding pyridoxal-5'-phosphate: G80 (= G72), M81 (= M73), Y105 (= Y97), D177 (= D168), S199 (= S190), T201 (= T192), K202 (= K193)

4iyoB Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
39% identity, 99% coverage: 5:374/375 of query aligns to 6:380/381 of 4iyoB

query
sites
4iyoB

T

T

Q

L

C

A

V

I

Q

H

A

G

G

G

T

Q

L

S

K

P

D

D

N

P

Q

S

Y

T

Q

G

G

A

L

V

N

M

S

P

P

P

L

I

Y

Y

T

A

S

T

T

S

A

T

Y

Y

G

A

Y

Q

M

S

D

S

K

P

E

G

E

E

V

H

C

Q

Y

G

P

F

R

E

Y
|
Y

F

S

T
x
R

T
|
T

-

H

-

N

P

P

N

T

Q

R

G

F

A

A

V

Y

V

E

K

R

K

C

I

V

C

A

A

A

L

L

E

E

G

G

A

G

E

T

D

R

G

A

L

F

L

A

F

F

S

A

S

S

G

G

M

M

A

A

A

A

V

T

S

S

T

T

T

-

I

V

L

M

A

E

H

L

T

L

R

D

S

A

G

G

D

S

H

H

V

V

I

V

L

A

L

M

D

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

H

F

S
x
R

M

L

A

-

T

-

A

-

D

-

F

F

E

E

R

R

L

V

-

R

-

R

-

R

-

T

-

A

G

G

L

L

E

D

Y

F

D

S

F

F

V

V

-

D

A

L

T

T

S

D

L

P

E

A

A

A

I

F

M

K

A

A

A

A

Q

I

K

R

P

A

N

D

T

T

T

K

M

M

V

V

I

W

I

I

E

E

S

T

P

P

T

T

N

N

P

P

L

M

M

L

N

K

I

L

L

V

D

D

I

I

K

A

A

A

T

I

A

A

D

V

W

I

A

A

R

R

E

K

N

H

K

G

I

L

T

L

S

T

L

V

M

V

D
|
D

N

N

T

T

F

F

G

A

T

S

S

P

I

M

N

L

Q

Q

T

R

P

P

L

L

A

S

L

L

G

G

I

A

D

D

I

L

V

V

V

V

H

H

S

S

G

A

T

T

K
|
K

Y

Y

M

L

G

N

G

G

H

H

S

S

D

D

V

M

C

V

C

G

G

G

F

I

A

A

L

V

A

V

-

G

-

D

S

N

K

A

K

E

K

L

M

A

Q

E

P
x
Q

I

M

L

A

D
x
F

L

L

A

Q

R
x
N

H

S

L

I

G

G

G

G

S

V

L

Q

D

G

S

P

F

F

A

D

A

S

Y

F

L

L

L

A

E

L

R

R

S

G

L

L

K

K

T

T

L

L

A

P

I

L

R

R

V

M

E

R

R

A

Q

H

C

C

Q

E

N

N

A

A

Q

L

A

A

L

L

A

A

E

Q

F

W

L

L

E

E

A

T

H

H

P

P

K

A

I

I

V

E

K

K

V

V

N

I

Y

Y

P

P

G

G

L

L

P

A

N

S

F

H

P

P

G

Q

H

H

D

V

I

L

A

A

K

K

K

R

Q

Q

M

M

K

S

L

G

F

F

G

G

A

G

M

I

M

V

S

S

F

I

E

V

L

L

D

K

E

G

S

G

I

F

G

D

D

A

V

A

D

K

V

R

F

F

L

C

R

E

K

K

L

T

K

E

V

L

I

F

H

T

P

L

A

A

V
x
E

S
|
S

L

L

G

G

G

G

V

V

E

E

S

S

S

L

I

V

C

N

S

H

P

P

K

A

D

V

T

M

S
x
T

H

H

K

A

P

S

M

I

T

P

A

V

E

A

E

R

R

R

A

E

R

Q

V

L

G

G

I

I

S

S

D

D

F

A

L

L

L

V

R
|
R

L

L

S

S

I

V

G

G

I

I

E

E

H

D

I

L

D

G

D

D

L

L

K

R

E

G

D

D

I

L

E

E

Q

R

A

A

L

L

active site: R47 (≠ T46), Y99 (= Y97), D172 (= D168), K197 (= K193)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y44), R47 (≠ T46)
binding amino-acrylate: Y99 (= Y97), K197 (= K193), S326 (= S320), T341 (≠ S335), R361 (= R355)
binding pyruvic acid: Q221 (≠ P215), F224 (≠ D218)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y97), R104 (≠ S102), N227 (≠ R221), E325 (≠ V319)

4iy7B Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
39% identity, 99% coverage: 5:374/375 of query aligns to 6:380/381 of 4iy7B

query
sites
4iy7B

T

T

Q

L

C

A

V

I

Q

H

A

G

G

G

T

Q

L

S

K

P

D

D

N

P

Q

S

Y

T

Q

G

G

A

L

V

N

M

S

P

P

P

L

I

Y

Y

T

A

S

T

T

S

A

T

Y

Y

G

A

Y

Q

M

S

D

S

K

P

E

G

E

E

V

H

C

Q

Y

G

P

F

R

E

Y
|
Y

F

S

T
x
R

T
|
T

-

H

-

N

P

P

N

T

Q

R

G

F

A

A

V

Y

V

E

K

R

K

C

I

V

C

A

A

A

L

L

E

E

G

G

A

G

E

T

D

R

G

A

L

F

L

A

F

F

S

A

S

S

G
|
G

M
|
M

A

A

A

A

V

T

S

S

T

T

T

-

I

V

L

M

A

E

H

L

T

L

R

D

S

A

G

G

D

S

H

H

V

V

I

V

L

A

L

M

D

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

H

F

S
x
R

M

L

A

-

T

-

A

-

D

-

F

F

E

E

R

R

L

V

-

R

-

R

-

R

-

T

-

A

G

G

L

L

E

D

Y

F

D

S

F

F

V

V

-

D

A

L

T

T

S

D

L

P

E

A

A

A

I

F

M

K

A

A

A

A

Q

I

K

R

P

A

N

D

T

T

T

K

M

M

V

V

I

W

I

I

E
|
E

S

T

P

P

T

T

N
|
N

P

P

L

M

M

L

N

K

I

L

L

V

D

D

I

I

K

A

A

A

T

I

A

A

D

V

W

I

A

A

R

R

E

K

N

H

K

G

I

L

T

L

S

T

L

V

M

V

D
|
D

N

N

T

T

F

F

G

A

T

S

S

P

I

M

N

L

Q

Q

T

R

P

P

L

L

A

S

L

L

G

G

I

A

D

D

I

L

V

V

V

V

H

H

S
|
S

G

A

T

T

K
|
K

Y

Y

M

L

G

N

G

G

H

H

S

S

D

D

V

M

C

V

C

G

G

G

F

I

A

A

L

V

A

V

-

G

-

D

S

N

K

A

K

E

K

L

M

A

Q

E

P
x
Q

I

M

L

A

D
x
F

L

L

A

Q

R
x
N

H

S

L

I

G

G

G

G

S

V

L

Q

D

G

S

P

F

F

A

D

A

S

Y

F

L

L

L

A

E

L

R

R

S

G

L

L

K

K

T

T

L

L

A

P

I

L

R

R

V

M

E

R

R

A

Q

H

C

C

Q

E

N

N

A

A

Q

L

A

A

L

L

A

A

E

Q

F

W

L

L

E

E

A

T

H

H

P

P

K

A

I

I

V

E

K

K

V

V

N

I

Y

Y

P

P

G

G

L

L

P

A

N

S

F

H

P

P

G

Q

H

H

D

V

I

L

A

A

K

K

K

R

Q

Q

M

M

K

S

L

G

F

F

G

G

A

G

M

I

M

V

S

S

F

I

E

V

L

L

D

K

E

G

S

G

I

F

G

D

D

A

V

A

D

K

V

R

F

F

L

C

R

E

K

K

L

T

K

E

V

L

I

F

H

T

P

L

A

A

V
x
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

S

L

I

V

C

N

S

H

P

P

K

A

D

V

T

M

S
x
T

H

H

K

A

P

S

M

I

T

P

A

V

E

A

E

R

R

R

A

E

R

Q

V

L

G

G

I

I

S

S

D

D

F

A

L

L

L

V

R
|
R

L

L

S

S

I

V

G

G

I

I

E

E

H

D

I

L

D

G

D

D

L

L

K

R

E

G

D

D

I

L

E

E

Q

R

A

A

L

L

active site: R47 (≠ T46), Y99 (= Y97), D172 (= D168), K197 (= K193)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y44), R47 (≠ T46)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: G75 (= G72), M76 (= M73), Y99 (= Y97), E143 (= E139), N147 (= N143), D172 (= D168), S194 (= S190), K197 (= K193), S326 (= S320), L327 (= L321), T341 (≠ S335), R361 (= R355)
binding pyruvic acid: Q221 (≠ P215), F224 (≠ D218)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y97), R104 (≠ S102), N227 (≠ R221), E325 (≠ V319)

4iy7A Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
39% identity, 99% coverage: 5:374/375 of query aligns to 6:380/381 of 4iy7A

query
sites
4iy7A

T

T

Q

L

C

A

V

I

Q

H

A

G

G

G

T

Q

L

S

K

P

D

D

N

P

Q

S

Y

T

Q

G

G

A

L

V

N

M

S

P

P

P

L

I

Y

Y

T

A

S

T

T

S

A

T

Y

Y

G

A

Y

Q

M

S

D

S

K

P

E

G

E

E

V

H

C

Q

Y

G

P

F

R

E

Y
|
Y

F

S

T
x
R

T
|
T

-

H

-

N

P

P

N

T

Q

R

G

F

A

A

V

Y

V

E

K

R

K

C

I

V

C

A

A

A

L

L

E

E

G

G

A

G

E

T

D

R

G

A

L

F

L

A

F

F

S

A

S

S

G
|
G

M
|
M

A

A

A

A

V

T

S

S

T

T

T

-

I

V

L

M

A

E

H

L

T

L

R

D

S

A

G

G

D

S

H

H

V

V

I

V

L

A

L

M

D

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

H

F

S
x
R

M

L

A

-

T

-

A

-

D

-

F

F

E

E

R

R

L

V

-

R

-

R

-

R

-

T

-

A

G

G

L

L

E

D

Y

F

D

S

F

F

V

V

-

D

A

L

T

T

S

D

L

P

E

A

A

A

I

F

M

K

A

A

A

A

Q

I

K

R

P

A

N

D

T

T

T

K

M

M

V

V

I

W

I

I

E
|
E

S

T

P

P

T

T

N
|
N

P

P

L

M

M

L

N

K

I

L

L

V

D

D

I

I

K

A

A

A

T

I

A

A

D

V

W

I

A

A

R

R

E

K

N

H

K

G

I

L

T

L

S

T

L

V

M

V

D
|
D

N

N

T

T

F

F

G

A

T

S

S

P

I

M

N

L

Q

Q

T

R

P

P

L

L

A

S

L

L

G

G

I

A

D

D

I

L

V

V

V

V

H

H

S
|
S

G

A

T

T

K
|
K

Y

Y

M

L

G

N

G

G

H

H

S

S

D

D

V

M

C

V

C

G

G

G

F

I

A

A

L

V

A

V

-

G

-

D

S

N

K

A

K

E

K

L

M

A

Q

E

P

Q

I

M

L

A

D

F

L

L

A

Q

R
x
N

H

S

L

I

G

G

G

G

S

V

L

Q

D

G

S

P

F

F

A

D

A

S

Y

F

L

L

L

A

E

L

R

R

S

G

L

L

K

K

T

T

L

L

A

P

I

L

R

R

V

M

E

R

R

A

Q

H

C

C

Q

E

N

N

A

A

Q

L

A

A

L

L

A

A

E

Q

F

W

L

L

E

E

A

T

H

H

P

P

K

A

I

I

V

E

K

K

V

V

N

I

Y

Y

P

P

G

G

L

L

P

A

N

S

F

H

P

P

G

Q

H

H

D

V

I

L

A

A

K

K

K

R

Q

Q

M

M

K

S

L

G

F

F

G

G

A

G

M

I

M

V

S

S

F

I

E

V

L

L

D

K

E

G

S

G

I

F

G

D

D

A

V

A

D

K

V

R

F

F

L

C

R

E

K

K

L

T

K

E

V

L

I

F

H

T

P

L

A

A

V
x
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

S

L

I

V

C

N

S

H

P

P

K

A

D

V

T

M

S
x
T

H

H

K

A

P

S

M

I

T

P

A

V

E

A

E

R

R

R

A

E

R

Q

V

L

G

G

I

I

S

S

D

D

F

A

L

L

L

V

R
|
R

L

L

S

S

I

V

G

G

I

I

E

E

H

D

I

L

D

G

D

D

L

L

K

R

E

G

D

D

I

L

E

E

Q

R

A

A

L

L

active site: R47 (≠ T46), Y99 (= Y97), D172 (= D168), K197 (= K193)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: G75 (= G72), M76 (= M73), Y99 (= Y97), E143 (= E139), N147 (= N143), D172 (= D168), S194 (= S190), K197 (= K193), S326 (= S320), L327 (= L321), T341 (≠ S335), R361 (= R355)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: Y45 (= Y44), R47 (≠ T46)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y97), R104 (≠ S102), N227 (≠ R221), E325 (≠ V319)

4ixzA Native structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae at ph 9.0 (see paper)
39% identity, 99% coverage: 5:374/375 of query aligns to 6:380/381 of 4ixzA

query
sites
4ixzA

T

T

Q

L

C

A

V

I

Q

H

A

G

G

G

T

Q

L

S

K

P

D

D

N

P

Q

S

Y

T

Q

G

G

A

L

V

N

M

S

P

P

P

L

I

Y

Y

T

A

S

T

T

S

A

T

Y

Y

G

A

Y

Q

M

S

D

S

K

P

E

G

E

E

V

H

C

Q

Y

G

P

F

R

E

Y

Y

F

S

T
x
R

T

T

-

H

-

N

P

P

N

T

Q

R

G

F

A

A

V

Y

V

E

K

R

K

C

I

V

C

A

A

A

L

L

E

E

G

G

A

G

E

T

D

R

G

A

L

F

L

A

F

F

S

A

S

S

G

G

M

M

A

A

A

A

V

T

S

S

T

T

T

-

I

V

L

M

A

E

H

L

T

L

R

D

S

A

G

G

D

S

H

H

V

V

I

V

L

A

L

M

D

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

H

F

S

R

M

L

A

-

T

-

A

-

D

-

F

F

E

E

R

R

L

V

-

R

-

R

-

R

-

T

-

A

G

G

L

L

E

D

Y

F

D

S

F

F

V

V

-

D

A

L

T

T

S

D

L

P

E

A

A

A

I

F

M

K

A

A

A

A

Q

I

K

R

P

A

N

D

T

T

T

K

M

M

V

V

I

W

I

I

E

E

S

T

P

P

T

T

N

N

P

P

L

M

M

L

N

K

I

L

L

V

D

D

I

I

K

A

A

A

T

I

A

A

D

V

W

I

A

A

R

R

E

K

N

H

K

G

I

L

T

L

S

T

L

V

M

V

D
|
D

N

N

T

T

F

F

G

A

T

S

S

P

I

M

N

L

Q

Q

T

R

P

P

L

L

A

S

L

L

G

G

I

A

D

D

I

L

V

V

V

V

H

H

S

S

G

A

T

T

K
|
K

Y

Y

M

L

G

N

G

G

H

H

S

S

D

D

V

M

C

V

C

G

G

G

F

I

A

A

L

V

A

V

-

G

-

D

S

N

K

A

K

E

K

L

M

A

Q

E

P

Q

I

M

L

A

D

F

L

L

A

Q

R

N

H

S

L

I

G

G

G

G

S

V

L

Q

D

G

S

P

F

F

A

D

A

S

Y

F

L

L

L

A

E

L

R

R

S

G

L

L

K

K

T

T

L

L

A

P

I

L

R

R

V

M

E

R

R

A

Q

H

C

C

Q

E

N

N

A

A

Q

L

A

A

L

L

A

A

E

Q

F

W

L

L

E

E

A

T

H

H

P

P

K

A

I

I

V

E

K

K

V

V

N

I

Y

Y

P

P

G

G

L

L

P

A

N

S

F

H

P

P

G

Q

H

H

D

V

I

L

A

A

K

K

K

R

Q

Q

M

M

K

S

L

G

F

F

G

G

A

G

M

I

M

V

S

S

F

I

E

V

L

L

D

K

E

G

S

G

I

F

G

D

D

A

V

A

D

K

V

R

F

F

L

C

R

E

K

K

L

T

K

E

V

L

I

F

H

T

P

L

A

A

V

E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

S

L

I

V

C

N

S

H

P

P

K

A

D

V

T

M

S
x
T

H

H

K

A

P

S

M

I

T

P

A

V

E

A

E

R

R

R

A

E

R

Q

V

L

G

G

I

I

S

S

D

D

F

A

L

L

L

V

R
|
R

L

L

S

S

I

V

G

G

I

I

E

E

H

D

I

L

D

G

D

D

L

L

K

R

E

G

D

D

I

L

E

E

Q

R

A

A

L

L

active site: R47 (≠ T46), Y99 (= Y97), D172 (= D168), K197 (= K193)
binding bicarbonate ion: Y99 (= Y97), S326 (= S320), L327 (= L321), T341 (≠ S335), R361 (= R355)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
37% identity, 99% coverage: 5:374/375 of query aligns to 9:391/395 of 5m3zA

query
sites
5m3zA

T

T

Q

Q

C

I

V

V

Q

H

A

A

G

G

T

Q

L

Q

K

P

D

D

N

P

Q

S

Y

T

Q

G

G

A

L

L

N

S

S

T

P

P

L

I

Y

F

T

Q

S

T

T

S

A

T

Y

F

G

V

Y

F

M

D

D

S

K

A

E

E

E

Q

-

G

-

A

-

A

-

R

-

F

-

A

-

L

-

E

-

E

-

S

-

G

V

Y

C

I

Y

Y

P

T

R
|
R

Y

-

F

L

T

G

T

N

P

P

N

T

Q

T

G

D

A

A

V

L

V

E

K

K

K

K

I

L

C

A

A

V

L

L

E

E

G

R

A

G

E

E

D

A

G

G

L

L

L

A

F

T

S

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

S

T

T

T

T

T

I

L

L

L

A

T

H

L

T

C

R

Q

S

Q

G

G

D

D

H

H

V

I

I

V

L

S

L

A

D

S

D

A

L

I

Y
|
Y

G

G

G
x
H

T

T

H

H

S

A

M

F

A

L

T

S

A

H

D

S

F

M

E

P

R

K

L

F

G

G

L

I

E

N

Y

V

D

S

F

F

V

V

-

D

A

A

T

A

S

K

L

P

E

E

A

E

I

I

M

R

A

A

A

A

Q

M

K

R

P

P

N

E

T

T

T

K

M

V

V

V

I

Y

I

I

E
|
E

S

T

P

P

T

A

N

N

P

P

L

T

M

L

N

S

I

L

L

V

D

D

I

I

K

E

A

T

T

V

A

A

D

G

W

I

A

A

R

H

E

Q

N

Q

K

G

I

A

T

L

S

L

L

V

M

V

D
|
D

N

N

T
|
T

F

F

G

M

T

S

S

P

I

Y

N

C

Q

Q

T

Q

P

P

L

L

A

Q

L

L

G

G

I

A

D

D

I

I

V

V

V

V

H

H

S
|
S

G

V

T
|
T

K
|
K

Y

Y

M

I

G

N

G

G

H

H

S

G

D

D

V

V

C

I

C

G

G

G

F

I

A

I

L

V

A

G

S

K

K

-

K

-

K

-

M

-

Q

Q

P

E

I

F

L

I

D

D

L

Q

A

A

R

R

H

F

L

V

G

G

-

L

-

K

-

D

-

I

-

T

-

G

G

G

S

C

L

M

D

S

S

P

F

F

A

N

A

A

Y

W

L

L

L

T

E

L

R

R

S

G

L

V

K

K

T

T

L

L

A

G

I

I

R

R

V

M

E

E

R

R

Q

H

C

C

Q

E

N

N

A

A

Q

L

A

K

L

I

A

A

E

R

F

F

L

L

E

E

A

G

H

H

P

P

K

S

I

I

V

T

K

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

P

S

N

S

F

H

P

P

G

Q

H

Y

D

E

I

L

A

G

K

Q

K

R

Q

Q

M

M

K

S

L

L

F

P

G

G

A

G

M

I

M

I

S

S

F

F

E

E

L

I

D

A

E

G

S

G

I

L

G

E

D

A

V

G

D

R

V

R

F

M

L

I

R

N

K

S

L

V

K

E

V

L

I

C

H

L

P

L

A

A

V
|
V

S
|
S

L

L

G

G

G

D

V

T

E

E

S

T

S

L

I

I

C

Q

S

H

P

P

K

A

D

S

T

M

S

T

H

H

K

S

P

P

M

V

T

A

A

P

E

E

E

E

R

R

A

L

R

K

V

A

G

G

I

I

S

T

D

D

F

G

L

L

L

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

H

D

I

P

D

E

D

D

L

I

K

I

E

N

D

D

I

L

E

E

Q

H

A

A

L

I

active site: R58 (= R43), Y111 (= Y97), D183 (= D168), K208 (= K193)
binding norleucine: Y111 (= Y97), H113 (≠ G99), K208 (= K193), V336 (= V319), S337 (= S320)
binding pyridoxal-5'-phosphate: G86 (= G72), I87 (≠ M73), Y111 (= Y97), E154 (= E139), D183 (= D168), T185 (= T170), S205 (= S190), T207 (= T192), K208 (= K193)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G72), I87 (≠ M73), Y111 (= Y97), D183 (= D168), S205 (= S190), T207 (= T192), K208 (= K193), V336 (= V319), S337 (= S320), R372 (= R355)

4iyoD Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
39% identity, 99% coverage: 5:374/375 of query aligns to 6:380/384 of 4iyoD

query
sites
4iyoD

T

T

Q

L

C

A

V

I

Q

H

A

G

G

G

T

Q

L

S

K

P

D

D

N

P

Q

S

Y

T

Q

G

G

A

L

V

N

M

S

P

P

P

L

I

Y

Y

T

A

S

T

T

S

A

T

Y

Y

G

A

Y

Q

M

S

D

S

K

P

E

G

E

E

V

H

C

Q

Y

G

P

F

R

E

Y
|
Y

F

S

T
x
R

T
|
T

-

H

-

N

P

P

N

T

Q

R

G

F

A

A

V

Y

V

E

K

R

K

C

I

V

C

A

A

A

L

L

E

E

G

G

A

G

E

T

D

R

G

A

L

F

L

A

F

F

S

A

S

S

G

G

M

M

A

A

A

A

V

T

S

S

T

T

T

-

I

V

L

M

A

E

H

L

T

L

R

D

S

A

G

G

D

S

H

H

V

V

I

V

L

A

L

M

D

D

D

D

L

L

Y
|
Y

G

G

G

G

T

T

H

F

S
x
R

M

L

A

-

T

-

A

-

D

-

F

F

E

E

R

R

L

V

-

R

-

R

-

R

-

T

-

A

G

G

L

L

E

D

Y

F

D

S

F

F

V

V

-

D

A

L

T

T

S

D

L

P

E

A

A

A

I

F

M

K

A

A

A

A

Q

I

K

R

P

A

N

D

T

T

T

K

M

M

V

V

I

W

I

I

E

E

S

T

P

P

T

T

N

N

P

P

L

M

M

L

N

K

I

L

L

V

D

D

I

I

K

A

A

A

T

I

A

A

D

V

W

I

A

A

R

R

E

K

N

H

K

G

I

L

T

L

S

T

L

V

M

V

D
|
D

N

N

T

T

F

F

G

A

T

S

S

P

I

M

N

L

Q

Q

T

R

P

P

L

L

A

S

L

L

G

G

I

A

D

D

I

L

V

V

V

V

H

H

S

S

G

A

T

T

K
|
K

Y

Y

M

L

G

N

G

G

H

H

S

S

D

D

V

M

C

V

C

G

G

G

F

I

A

A

L

V

A

V

S

G

K

D

K

N

K

A

-

E

-

L

M

A

Q

E

P

Q

I

M

L

A

D

F

L

L

A

Q

R
x
N

H

S

L

I

G

G

G

G

S

V

L

Q

D

G

S

P

F

F

A

D

A

S

Y

F

L

L

L

A

E

L

R

R

S

G

L

L

K

K

T

T

L

L

A

P