SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028314758.1 NCBI__GCF_000429905.1:WP_028314758.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

5odqB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with bromoethanesulfonate. (see paper)
27% identity, 52% coverage: 165:375/409 of query aligns to 4:235/291 of 5odqB

query
sites
5odqB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G169), C9 (= C170), C41 (= C202), C42 (= C203), C78 (= C239), G80 (≠ T241), C81 (= C242), G152 (≠ P299), C153 (= C300), H154 (= H301), C193 (= C333), C194 (= C334), C231 (= C371), F233 (≠ A373), C234 (= C374)
binding 2-bromanylethanesulfonic acid: G46 (≠ A207), G80 (≠ T241), H154 (= H301), G197 (= G337), R201 (≠ F341), F233 (≠ A373)

Sites not aligning to the query:

binding 2-bromanylethanesulfonic acid: 236

5odhH Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
27% identity, 52% coverage: 165:375/409 of query aligns to 4:235/291 of 5odhH

query
sites
5odhH

A

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G169), C9 (= C170), C41 (= C202), C42 (= C203), C78 (= C239), G80 (≠ T241), C81 (= C242), C153 (= C300), H154 (= H301), C193 (= C333), C194 (= C334), C231 (= C371), F233 (≠ A373), C234 (= C374)
binding 1-thioethanesulfonic acid: A44 (≠ M205), P45 (= P206), G46 (≠ A207), G80 (≠ T241), H154 (= H301), G197 (= G337), G198 (= G338)
binding Coenzyme B: R201 (≠ F341), F233 (≠ A373)

Sites not aligning to the query:

binding Coenzyme B: 240

5odhB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
27% identity, 52% coverage: 165:375/409 of query aligns to 4:235/291 of 5odhB

query
sites
5odhB

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G169), C9 (= C170), I10 (≠ A171), C41 (= C202), C42 (= C203), C78 (= C239), G80 (≠ T241), C81 (= C242), G152 (≠ P299), C153 (= C300), H154 (= H301), C193 (= C333), C194 (= C334), C231 (= C371), F233 (≠ A373), C234 (= C374)
binding 1-thioethanesulfonic acid: P45 (= P206), G46 (≠ A207), G80 (≠ T241), F233 (≠ A373)

5odcB Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
27% identity, 52% coverage: 165:375/409 of query aligns to 4:235/291 of 5odcB

query
sites
5odcB

A

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binding Non-cubane [4Fe-4S]-cluster: G8 (= G169), C9 (= C170), I10 (≠ A171), C41 (= C202), C42 (= C203), C78 (= C239), N79 (≠ T240), G80 (≠ T241), C81 (= C242), G152 (≠ P299), C153 (= C300), C193 (= C333), C194 (= C334), G197 (= G337), C231 (= C371), F233 (≠ A373), C234 (= C374)

Query Sequence

>WP_028314758.1 NCBI__GCF_000429905.1:WP_028314758.1
MSFDLHDFEEMMAKCSHCAFCQATCPVFLQDLLETHMPRARMTLAKACLMDQEVPRTKRL
EKIMDRCLLCGNCERTCPAGVKVNDVVVAARKDLYKGKRKSAIERNFLSSFMQKRGMDRL
HRVGYSLAKKMGKVPDGLLEPRADTFSQQFTGVFPAEGEKRATVAYFVGCATNSSFVETG
KALINVYTRNGIEVIVPEGLLCCGMPAIAEGDVETAYNMFMHNIRIFHKMEVDAVVTDCT
TCGLMFKENGVKLAPAGTPLVEKVQGVAAKIWEATDYLNHLGLVDQPAGLDLKFTYHEPC
HRAWSPTLADAPRAVTKGIPGAELCEMAAPDACCGAGGTFFVQHPELSGGIRSKKTDMIE
KTGADAILTQCPACRGYLAAGCEEKTVMHPLDLLAQAYKVHNKTPTPRE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory