SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028315507.1 NCBI__GCF_000429905.1:WP_028315507.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
31% identity, 96% coverage: 12:360/365 of query aligns to 7:376/386 of P0A9J8

query
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P0A9J8

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R11 (= R16) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R33) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K44) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E57) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (= Q92) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
32% identity, 73% coverage: 97:361/365 of query aligns to 4:275/278 of 2qmxA

query
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2qmxA

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active site: T169 (= T264), F171 (= F266)
binding phenylalanine: N204 (≠ H290), E205 (≠ I291), L209 (= L295), D222 (≠ N308), L223 (≠ M309), T224 (≠ V310), K225 (= K311), S228 (= S314), Y238 (= Y324), F240 (= F326)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
33% identity, 73% coverage: 99:364/365 of query aligns to 5:279/306 of 3mwbA

query
sites
3mwbA

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active site: T176 (= T264), F178 (= F266)
binding phenylalanine: D205 (vs. gap), H206 (≠ I291), L210 (= L295), N223 (= N308), L224 (≠ M309), S225 (≠ V310), R226 (≠ K311), S229 (= S314), F241 (= F326)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
33% identity, 73% coverage: 99:364/365 of query aligns to 5:276/303 of 3mwbB

query
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3mwbB

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active site: T176 (= T264), F178 (= F266)
binding magnesium ion: A155 (= A243), Q158 (≠ T246), L161 (= L249)
binding phenylalanine: D205 (vs. gap), H206 (≠ I291), L210 (= L295), N223 (= N308), L224 (≠ M309), S225 (≠ V310), R226 (≠ K311), I227 (≠ L312), Y236 (= Y324), F238 (= F326)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
30% identity, 66% coverage: 123:363/365 of query aligns to 34:280/282 of 6vh5D

query
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6vh5D

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A

F

F

E

N

E

A

V

V

D

E

K

T

R

G

R

A

P

D

F

L

G

A

V

M

V

I

P

P

V

I

E

E

N

N

S

T

M

L

E

A

G

G

A

R

V

V

N

A

L

D

T

I

L

H

D

Y

L

L

F

L

Q

P

E

L

S

A

D

D

V

M

R

H

I

I

C

V

G

G

E

E

I

Y

Y

F

L

L

P

P

I

I

R

H

H

F

D

Q

L

L

L

M

S

V

A

L

E

P

D

G

-

V

S

R

L

R

D

E

Q

E

I

I

M

K

V

T

V

V

F

H

S

S

H

H

P

I

Q

H

A

A

I

L

A

G

Q

Q

C

C

R

R

K

N

W

V

L

I

G

R

K

Q

N

N

-

G

L

W

P

K

G

G

I

V

L

I

Q

-

N

-

Q

-

C

A

S

G

S

D

T

T

S

A

E

G

A

A

R

R

M

L

A

V

-

A

-

D

V

V

N

K

T

D

P

R

G

S

G

M

A

A

I

L

A

A

S

P

S

R

E

L

A

A

I

D

T

L

Y

Y

E

G

L

L

N

D

T

I

L

L

A

E

D

E

N

N

I

V

Q

E

D

D

S

S

T

E

T

N

N

N

I

V

T

T

R

R

F

F

L

V

V

V

I

L

G

S

R

K

D

N

K

K

-

Q

-

W

-

A

V

A

Q

R

P

P

T

E

G

N

R

D

D

E

K

R

-

I

-

V

T

T

S

T

L

F

L

V

F

F

A

R

I

V

P

R

H
x
N

I
x
V

P

P

G

A

A

A

L
|
L

H

Y

S

K

V

A

L

L

Q

G

P

G

I

F

S

A

E

T

E

N

G

G

L

V

N
|
N

M
|
M

V
x
T

K
|
K

L

L

E

E

S
|
S

R

Y

P

Q

S

L

K

G

S

G

A

R

S

F

W

I

H

A

Y
x
T

L

Q

F
|
F

F

Y

V

A

D

D

L

I

E

E

G

G

H

H

M

P

K

E

D

E

E

R

P

S

V

V

K

Q

K

L

V

A

V

L

D

E

K

E

M

L

R

R

S

F

L

F

C

T

S

K

F

E

V

V

K

R

W

I

M

L

G

G

S

V

Y

Y

P

K

V

G

G

S

D

D

binding phenylalanine: N207 (≠ H290), V208 (≠ I291), L212 (= L295), N225 (= N308), M226 (= M309), T227 (≠ V310), K228 (= K311), S231 (= S314), T241 (≠ Y324), F243 (= F326)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
29% identity, 65% coverage: 123:361/365 of query aligns to 29:271/278 of 7am0B

query
sites
7am0B

P

P

L

C

A

Q

T

T

I

F

S

A

D

Q

V

T

F

I

E

A

V

V

D

H

K

D

R

G

R

R

R

A

P

E

F

L

G

A

V

M

V

L

P

A

V

C

E

E

N

N

S

S

M

L

E

A

G

G

A

R

V

V

N

P

L

D

T

I

L

H

D

A

L

L

F

L

Q

P

E

E

S

A

D

G

V

L

R

F

I

I

C

V

G

G

E

E

I

H

Y

F

L

Q

P

R

I

V

R

E

H

H

D

C

L

L

S

G

A

I

E

P

D

G

S

S

-

T

L

L

D

A

Q

D

I

A

M

R

V

R

V

I

F

H

S

T

H

H

P

P

Q

V

A

A

I

M

A

A

Q

Q

C

V

R

R

K

G

W

I

L

I

G

T

K

E

-

L

N

G

L

L

P

D

G

P

I

V

L

V

Q

E

N

F

Q

D

C

T

S

A

S

G

T

A

S

A

E

E

A

M

A

V

R

R

M

-

A

E

V

W

N

G

T

R

P

K

G

E

G

D

A

V

A

A

I

V

A

A

S

S

S

A

E

L

A

A

I

E

T

L

Y

N

E

G

L

L

N

E

T

I

L

L

A

R

D

R

N

N

I

V

Q

E

D

D

S

A

T

T

T

H

N

N

I

T

T

T

R

R

F

F

L

Y

V

I

I

A

G

S

R

R

D

R

-

P

-

A

K

T

V

L

Q

P

P

P

T

P

G

G

R

P

D

G

-

F

K

M

T

T

S

T

L

L

F

F

A

R

I

V

P
x
N

H
x
N

I
x
Q

P
|
P

G

G

A

A

L
|
L

H

Y

S

K

V

A

L

L

Q

G

P

G

I

L

S

A

E

T

E

A

G

G

L

V

N
|
N

M
|
M

V
x
T

K
x
R

L

L

E

E

S

S

R

Y

P

M

S

L

K

E

S

G

A

S

S

F

W

S

H

A

Y
x
T

L

Q

F
|
F

F

L

V

M

D

D

L

V

E

E

G

G

H

H

M

P

K

E

D

A

E

P

P

P

V

L

K

A

K

R

V

A

V

L

D

D

K

E

M

L

R

S

S

F

L

F

C

S

S

E

F

Q

V

Q

K

E

W

I

M

L

G

G

S

V

Y

Y

P

P

V

A

binding phenylalanine: N199 (≠ P289), N200 (≠ H290), Q201 (≠ I291), P202 (= P292), L205 (= L295), N218 (= N308), M219 (= M309), T220 (≠ V310), R221 (≠ K311), T234 (≠ Y324), F236 (= F326)

3luyA Putative chorismate mutase from bifidobacterium adolescentis
27% identity, 73% coverage: 99:365/365 of query aligns to 8:289/326 of 3luyA

query
sites
3luyA

Y

Y

L

L

G

G

P

P

K

Q

A

G

T

T

F

F

T

T

H

H

M

Q

A

A

M

V

K

N

H

A

F

A

G

Q

R

E

S

L

T

A

N

R

F

F

T

E

P

P

-

Q

-

G

-

F

-

D

L

L

A

M

T

P

I

M

S

D

D

D

V

V

F

P

E

Q

E

I

V

L

D

D

K

A

R

A

R

Q

R

H

P

G

-

D

-

G

F

W

G

G

V

I

V

V

P

A

V

W

E

E

N

N

S

N

M

V

E

E

G

G

A

Y

V

V

N

V

L

P

T

N

L

L

D

D

-

A

L

L

F

I

Q

D

E

A

S

K

D

D

V

L

R

V

I

G

C

F

G

A

E

R

I

V

Y

G

L

V

P

N

I

V

R

E

H

F

D

D

L

A

L

Y

S

V

A

A

E

Q

D

G

S

A

-

D

L

P

D

A

Q

E

I

A

M

R

V

I

V

A

F

T

S

A

H

H

P

P

Q

H

A

G

I

L

A

A

Q

Q

C

C

R

K

K

R

W

F

L

I

G

A

K

E

N

H

L

R

P

-

G

-

I

-

L

L

Q

S

N

T

Q

Q

C

-

S

P

S

A

T

T

S

S

E

N

A

A

R

A

M

C

A

R

V

D

N

L

T

I

P

P

G

G

G

E

A

I

A

A

I

F

A

G

S

P

S

A

E

I

A

C

A

G

I

E

T

L

Y

Y

E

D

L

I

N

T

T

R

L

I

A

G

D

T

N

A

I

I

Q

Q

D

D

S

Y

T

Q

T

G

N

A

I

A

T
|
T

R

D

F

F

L

L

V

V

-

L

-

S

-

P

-

R

-

A

-

E

I

V

G

A

R

R

D

L

K

L

V

A

Q

K

P

P

T

R

G

A

R

E

-

A

-

N

-

V

D

E

K

Y

T

E

S

S

L

V

L

L

F

T

A

L

I

I

P

P

H

L

I

V

-

T

-
x
G

P

P

G

G

A

V

L
|
L

H

A

S

N

V

L

L

L

Q

D

P

V

I

F

S

R

E

D

E

A

G

G

L

L

N

N

M

M

V

T

K

S

L

F

E

I

S

S

R

R

P

P

S

I

K

K

S

G

A

R

S

T

W

G

H

T

Y
|
Y

L

S

F
|
F

F

I

V

V

D

T

L

L

E

D

G

A

H

A

M

P

K

W

D

E

E

E

P

R

V

F

K

R

K

D

V

A

V

L

D

V

K

E

M

I

R

A

S

E

L

H

C

G

S

D

F

W

V

A

K

K

W

T

M

L

G

A

S

V

Y

Y

P

P

V

R

G

R

D

E

R

H

P

P

active site: T177 (= T264)
binding 3-phenylpyruvic acid: G215 (vs. gap), L219 (= L295), Y248 (= Y324), F250 (= F326)

P39912 Protein AroA(G); EC 2.5.1.54; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see paper)
39% identity, 23% coverage: 11:93/365 of query aligns to 5:87/358 of P39912

query
sites
P39912

E

E

L

L

E

E

P

L

L

L

R

R

Q

Q

A

K

I

A

N

D

E

E

T

L

D

N

Q

L

K

Q

I

I

L

L

A

K

L

L

V

I

N

N

E

E

R

R

L

G

D

N

I

V

A

V

Q

K

K

E

I

I

G

G

A

K

I

A

K

K

Q

E

G

A

K

Q

G

G

L

V

P

N

V

R

V

F

D

D

F

P

S

V

R

R

E

E

R

R

A

T

V

M

L

L

N

N

K

N

L

I

Q

I

E

E

L

N

N

N

E

D

G

G

P

P

M

F

P

E

D

N

E

S

T

T

L

I

L

Q

L

H

L

I

Y

F

T

K

E

E

I

I

I

F

A

K

A

A

S

G

R

L

R

E

I

L

Q

Q

Q

E

Sites not aligning to the query:

2 modified: Phosphoserine

5gmuB Crystal structure of chorismate mutase like domain of bifunctional dahp synthase of bacillus subtilis in complex with chlorogenic acid (see paper)
39% identity, 23% coverage: 11:93/365 of query aligns to 4:86/87 of 5gmuB

query
sites
5gmuB

E

E

L

L

E

E

P

L

L

L

R

R

Q

Q

A

K

I

A

N

D

E

E

T

L

D

N

Q

L

K

Q

I

I

L

L

A

K

L

L

V

I

N

N

E

E

R
|
R

L

G

D

N

I

V

A

V

Q

K

K

E

I

I

G

G

A

K

I

A

K

K

Q

E

G

A

K

Q

G

G

L

V

P

N

V
x
R

V
x
F

D
|
D

F

P

S

V

R

R

E
|
E

R

R

A

T

V

M

L

L

N

N

K

N

L

I

Q

I

E

E

L

N

N

N

E

D

G

G

P

P

M

F

P

E

D

N

E

S

T

T

L

I

L

Q

L

H

L

I

Y

F

T

K

E

E

I

I

I
x
F

A
x
K

A

A

S

G

R
x
L

R

E

I

L

Q
|
Q

Q

E

binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: R26 (= R33), R44 (≠ V51), F45 (≠ V52), D46 (= D53), E50 (= E57), F78 (≠ I85), K79 (≠ A86), L82 (≠ R89), Q85 (= Q92)

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
37% identity, 22% coverage: 12:93/365 of query aligns to 4:85/352 of 5j6fA

query
sites
5j6fA

L

L

E

D

P

E

L

L

R

R

Q

A

A

R

I

V

N

D

E

E

T

I

D

N

Q

L

K

Q

I

L

L

L

A

K

L

L

V

I

N

N

E

E

R
|
R

L

G

D

R

I

L

A

V

Q

Q

K

E

I
|
I

G

G

A

K

I

I

K
|
K

Q

E

G

A

K

Q

G

G

L

T

P

H

V

R

V
x
Y

D
|
D

F

P

S

V

R
|
R

E

E

R

R

A

K

V
x
M

L

L

N

D

K

L

L

I

Q

S

E

E

L

H

N

N

E

D

G

G

P

P

M

F

P

E

D

T

E

S

T

T

L

L

L

Q

L

H

L

I

Y

F

T

K

E

E

I

I

I

F

A

K

A

A

S

A

R

L

R

E

I

L

Q

Q

Q

E

binding prephenic acid: R25 (= R33), I32 (= I40), K36 (= K44), Y44 (≠ V52), D45 (= D53), R48 (= R56), M52 (≠ V60)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
31% identity, 27% coverage: 262:361/365 of query aligns to 85:187/194 of 7alzA

query
sites
7alzA

N

N

I

T

T

T

R

R

F

F

L

Y

V

I

I

A

G

S

R

R

D

R

-

P

-

A

K

T

V

L

Q

P

P

P

T

P

G

G

R

P

D

G

-

F

K

M

T

T

S

T

L

L

F

F

A

R

I

V

P

N

H

N

I
x
Q

P

P

G

G

A

A

L
|
L

H

Y

S

K

V

A

L

L

Q

G

P

G

I

L

S

A

E

T

E

A

G

G

L

V

N
|
N

M
|
M

V
x
T

K
x
R

L

L

E

E

S

S

R

Y

P

M

S

L

K

E

S

G

A

S

S

F

W

S

H

A

Y
x
T

L

Q

F
|
F

F

L

V

M

D

D

L

V

E

E

G

G

H

H

M

P

K

E

D

A

E

P

P

P

V

L

K

A

K

R

V

A

V

L

D

D

K

E

M

L

R

S

S

F

L

F

C

S

S

E

F

Q

V

Q

K

E

W

I

M

L

G

G

S

V

Y

Y

P

P

V

A

binding phenylalanine: Q117 (≠ I291), L121 (= L295), N134 (= N308), M135 (= M309), T136 (≠ V310), R137 (≠ K311), T150 (≠ Y324), F152 (= F326)

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
35% identity, 22% coverage: 15:93/365 of query aligns to 2:75/345 of 3nvtA

query
sites
3nvtA

L

L

R

R

Q

T

A

Q

I

V

N

D

E

Q

T

L

D

N

Q

I

K

D

I

L

L

L

A

E

L

L

V

I

N

S

E

K

R

R

L

A

D

N

I

L

A

V

Q

Q

K

E

I

I

G

G

A

K

I

I

K

K

Q

-

G

G

K

R

G

F

L

D

P

P

V

L

V

-

D

-

F

-

S

-

R

R

E

E

R

R

A

E

V

M

L

L

N

N

K

T

L

I

Q

L

E

A

L

A

N

N

E

E

G

G

P

P

M

F

P

E

D

D

E

S

T

T

L

V

L

Q

L

K

L

L

Y

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T

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E

I

I

I

F

A

K

A

A

S

G

R

L

R

E

I

L

Q

Q

Q

E

Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

6al9B Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
35% identity, 22% coverage: 12:93/365 of query aligns to 5:84/91 of 6al9B

query
sites
6al9B

L

L

E

E

P

N

L

L

R

R

Q

A

A

E

I

I

N

D

E

A

T

L

D

D

Q

N

K

E

I

L

L

S

A

D

L

L

V

L

N

D

E

K

R

R

L

L

D

E

I

I

A

A

Q

L

K

K

I
|
I

G

A

A

L

I

I

K

K

Q

Q

G

E

K

S

G

-

L

-

P

P

V

I

V

Y

D

C

F

P

S

K

R

R

E

E

R

Q

A

E

V

I

L

L

N

K

K

R

L

L

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S

E

Q

L

R

N

D

E

F

G

K

P

H

M

L

P

N

D

G

E

E

T

I

L

L

L

T

L

G

L

F

Y

Y

T

T

E

E

I

V

I

F

A

K

A

I

S

S

R

R

R

K

I

F

Q
|
Q

Q

E

binding pyruvic acid: I33 (= I40), Q83 (= Q92)

6al9A Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
35% identity, 22% coverage: 12:93/365 of query aligns to 4:83/90 of 6al9A

query
sites
6al9A

L

L

E

E

P

N

L

L

R

R

Q

A

A

E

I

I

N

D

E

A

T

L

D

D

Q

N

K

E

I

L

L

S

A

D

L

L

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L

N

D

E

K

R
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R

L

L

D

E

I

I

A

A

Q

L

K

K

I

I

G

A

A

L

I

I

K

K

Q

Q

G

E

K

S

G

-

L

-

P

P

V

I

V

Y

D
x
C

F

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S

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R
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R

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E

R

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A

E

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L

L

N

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L

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Q

L

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F

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H

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L

L

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L

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Y

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I
x
F

A

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S

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binding prephenic acid: R25 (= R33), C43 (≠ D53), R46 (= R56), E47 (= E57), F75 (≠ I85), S78 (= S88), Q82 (= Q92)

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
34% identity, 22% coverage: 15:93/365 of query aligns to 1:74/343 of 3tfcA

query
sites
3tfcA

L

L

R

R

Q

T

A

Q

I

V

N

D

E

Q

T

L

D

N

Q

I

K

D

I

L

L

L

A

E

L

L

V

I

N

S

E

K

R

R

L

A

D

N

I

L

A

V

Q

Q

K

E

I

I

G

G

A

K

I

I

K

K

Q

L

G

R

K

-

G

-

L

-

P

-

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-

V

F

D

D

F

P

S

L

R

R

E

E

R

R

A

E

V

M

L

L

N

N

K

T

L

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L

E

A

L

A

N

N

E

E

G

G

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P

M

F

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D

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L

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I

I

I

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A

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L

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E

I

L

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Q

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Sites not aligning to the query:

binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

Query Sequence

>WP_028315507.1 NCBI__GCF_000429905.1:WP_028315507.1
MKPSNKNGLPELEPLRQAINETDQKILALVNERLDIAQKIGAIKQGKGLPVVDFSRERAV
LNKLQELNEGPMPDETLLLLYTEIIAASRRIQQPLEVGYLGPKATFTHMAAMKHFGRSTN
FTPLATISDVFEEVDKRRRPFGVVPVENSMEGAVNLTLDLFQESDVRICGEIYLPIRHDL
LSAEDSLDQIMVVFSHPQAIAQCRKWLGKNLPGILQNQCSSTSEAARMAVNTPGGAAIAS
SEAAITYELNTLADNIQDSTTNITRFLVIGRDKVQPTGRDKTSLLFAIPHIPGALHSVLQ
PISEEGLNMVKLESRPSKSASWHYLFFVDLEGHMKDEPVKKVVDKMRSLCSFVKWMGSYP
VGDRP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory