SitesBLAST
Comparing WP_028316922.1 NCBI__GCF_000429905.1:WP_028316922.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8VBT2 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Mus musculus (Mouse)
44% identity, 99% coverage: 4:309/309 of query aligns to 7:322/327 of Q8VBT2
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
1pwhA Rat liver l-serine dehydratase- complex with pyridoxyl-(o-methyl- serine)-5-monophosphate (see paper)
45% identity, 99% coverage: 4:309/309 of query aligns to 7:322/327 of 1pwhA
- active site: K41 (= K38), A65 (= A62), E194 (= E189), A198 (= A193), S200 (= S195), A222 (≠ S217), A269 (= A264), C303 (= C290)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-o-methyl-l-serine: F40 (= F37), K41 (= K38), S64 (= S61), A65 (= A62), N67 (= N64), A68 (= A65), F136 (= F132), G168 (= G163), G169 (= G164), G170 (= G165), G171 (= G166), A222 (≠ S217), G224 (= G219), C303 (= C290), G304 (= G291)
2rkbA Serine dehydratase like-1 from human cancer cells (see paper)
44% identity, 99% coverage: 3:308/309 of query aligns to 3:317/318 of 2rkbA
- active site: K38 (= K38), G62 (≠ A62), E190 (= E189), A194 (= A193), C196 (≠ S195), S218 (= S217), A265 (= A264), C299 (= C290)
- binding pyridoxal-5'-phosphate: F37 (= F37), K38 (= K38), N64 (= N64), G164 (= G164), G165 (= G165), G166 (= G166), G167 (≠ L167), S218 (= S217), C299 (= C290)
Q96GA7 Serine dehydratase-like; Cancerous serine dehydratase; cSDH; Glutamate racemase; L-serine deaminase; L-serine dehydratase/L-threonine deaminase; SDHL; L-threonine dehydratase; TDH; Serine dehydratase 2; SDH 2; EC 5.1.1.3; EC 4.3.1.17; EC 4.3.1.19 from Homo sapiens (Human) (see 2 papers)
44% identity, 99% coverage: 3:308/309 of query aligns to 13:327/329 of Q96GA7
- K48 (= K38) modified: N6-(pyridoxal phosphate)lysine
- G72 (≠ A62) mutation to A: Strongly increased enzyme activity towards threonine.
- L287 (vs. gap) mutation to V: Almost no change in Km and Vmax for serine and threonine. Significantly increased protein levels.
- C309 (= C290) mutation to A: Loss of enzyme activity.
P09367 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Rattus norvegicus (Rat) (see 3 papers)
40% identity, 99% coverage: 4:309/309 of query aligns to 7:358/363 of P09367
- K41 (= K38) modified: N6-(pyridoxal phosphate)lysine
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P20132 L-serine dehydratase/L-threonine deaminase; SDH; Hepatic serine dehydratase; hSDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Homo sapiens (Human) (see 3 papers)
44% identity, 99% coverage: 3:309/309 of query aligns to 6:322/328 of P20132
- K41 (= K38) modified: N6-(pyridoxal phosphate)lysine
- P128 (≠ K125) binding pyridoxal 5'-phosphate; mutation Missing: Reduced Km and Vmax for both L-serine and L-threonine. 10-fold increase in dissociation constant for pyridoxal-phosphate.
- C303 (= C290) mutation to A: Loss of enzyme activity.
1p5jA Crystal structure analysis of human serine dehydratase (see paper)
44% identity, 99% coverage: 3:309/309 of query aligns to 3:319/319 of 1p5jA
- active site: K38 (= K38), A62 (= A62), E191 (= E189), A195 (= A193), S197 (= S195), A219 (≠ S217), C300 (= C290)
- binding pyridoxal-5'-phosphate: F37 (= F37), K38 (= K38), N64 (= N64), V164 (= V162), G165 (= G163), G166 (= G164), G167 (= G165), G168 (= G166), A219 (≠ S217), C300 (= C290), G301 (= G291), G302 (= G292)
5c3uA Crystal structure of a fungal l-serine ammonia-lyase from rhizomucor miehei (see paper)
40% identity, 98% coverage: 4:307/309 of query aligns to 3:314/315 of 5c3uA
- active site: K37 (= K38), G63 (≠ A62), E191 (= E189), S195 (≠ A193), A197 (≠ S195), S219 (= S217), T297 (≠ C290)
- binding pyridoxal-5'-phosphate: K37 (= K38), N65 (= N64), S132 (≠ F132), P163 (≠ S161), G165 (= G163), G166 (= G164), G167 (= G165), G168 (= G166), S219 (= S217), T297 (≠ C290)
8y1jA Structure of the pyridoxal 5'-phosphate-dependent (plp) threonine deaminase ilva1 from pseudomonas aeruginosa pao1 (see paper)
29% identity, 96% coverage: 4:300/309 of query aligns to 16:313/502 of 8y1jA
Sites not aligning to the query:
Q9GZT4 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Homo sapiens (Human) (see 4 papers)
29% identity, 98% coverage: 4:306/309 of query aligns to 23:328/340 of Q9GZT4
- S31 (≠ H13) binding ATP
- S32 (≠ R14) binding ATP
- I33 (≠ L15) binding ATP
- K51 (≠ P33) binding ATP
- T52 (≠ P34) binding ATP
- K56 (= K38) modified: N6-(pyridoxal phosphate)lysine
- P69 (≠ Q47) binding Ca(2+)
- T81 (≠ S59) binding Ca(2+)
- N86 (= N64) binding pyridoxal 5'-phosphate
- Q89 (≠ Y67) binding ATP
- Y121 (≠ K100) binding ATP
- D178 (= D156) binding Mg(2+)
- G185 (= G163) binding pyridoxal 5'-phosphate
- G186 (= G164) binding pyridoxal 5'-phosphate
- G187 (= G165) binding pyridoxal 5'-phosphate
- G188 (= G166) binding pyridoxal 5'-phosphate
- M189 (≠ L167) binding pyridoxal 5'-phosphate
- E210 (= E189) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- A214 (= A193) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- D216 (≠ S195) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- N247 (≠ A227) binding Ca(2+); binding Mg(2+)
- K279 (= K258) binding ATP
- S313 (≠ C290) binding pyridoxal 5'-phosphate
- N316 (≠ G294) binding ATP
Sites not aligning to the query:
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
29% identity, 96% coverage: 4:301/309 of query aligns to 20:320/320 of 7nbhAAA
- active site: K53 (= K38), S81 (≠ A62), E207 (= E189), A211 (= A193), D213 (≠ S195), G236 (= G219), L309 (≠ V289), S310 (≠ C290)
- binding calcium ion: E207 (= E189), A211 (= A193), D213 (≠ S195)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ A62), G85 (= G66), Q86 (≠ Y67), K111 (≠ R93), I115 (≠ Q97), Y118 (≠ K100), D235 (≠ Q216), P281 (= P263), N313 (≠ G294), V314 (= V295), D315 (≠ T296)
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
29% identity, 97% coverage: 4:303/309 of query aligns to 20:322/322 of 7nbgAAA
- active site: K53 (= K38), S81 (≠ A62), E207 (= E189), A211 (= A193), D213 (≠ S195), G236 (= G219), L309 (≠ V289), S310 (≠ C290)
- binding calcium ion: E207 (= E189), A211 (= A193), D213 (≠ S195)
- binding pyridoxal-5'-phosphate: F52 (= F37), K53 (= K38), N83 (= N64), G182 (= G163), G183 (= G164), G184 (= G165), G185 (= G166), M186 (≠ L167), G236 (= G219), V237 (≠ A220), T282 (≠ A264), S310 (≠ C290), G311 (= G291)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ A62), G85 (= G66), Q86 (≠ Y67), I101 (≠ V82), K111 (≠ R93), I115 (≠ Q97), Y118 (≠ K100)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
29% identity, 97% coverage: 4:303/309 of query aligns to 20:322/323 of 7nbfAAA
- active site: K53 (= K38), S81 (≠ A62), E207 (= E189), A211 (= A193), D213 (≠ S195), G236 (= G219), L309 (≠ V289), S310 (≠ C290)
- binding calcium ion: E207 (= E189), A211 (= A193), D213 (≠ S195)
- binding magnesium ion: N244 (≠ A227)
- binding pyridoxal-5'-phosphate: F52 (= F37), K53 (= K38), N83 (= N64), G182 (= G163), G183 (= G164), G184 (= G165), G185 (= G166), M186 (≠ L167), G236 (= G219), V237 (≠ A220), T282 (≠ A264), S310 (≠ C290), G311 (= G291)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (= H5), L22 (≠ I6), T23 (= T8), P24 (= P9), L26 (= L11), T27 (≠ E12), F46 (≠ L31)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
29% identity, 97% coverage: 4:303/309 of query aligns to 20:322/323 of 7nbdAAA