SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
31% identity, 86% coverage: 18:228/246 of query aligns to 11:213/304 of Q96XT4

query
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Q96XT4

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C12 (= C19) binding [4Fe-4S] cluster
C15 (= C22) binding [4Fe-4S] cluster
IGCS 44:47 (≠ VGCS 50:53) binding thiamine diphosphate
C46 (= C52) binding [4Fe-4S] cluster
D90 (= D94) binding Mg(2+)
GD 91:92 (= GD 95:96) binding thiamine diphosphate
N118 (= N122) binding Mg(2+)
V120 (= V124) binding Mg(2+)
GL 122:123 (≠ GM 126:127) binding thiamine diphosphate
C197 (= C213) binding [4Fe-4S] cluster

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
27% identity, 91% coverage: 18:241/246 of query aligns to 7:237/286 of 5b48B

query
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5b48B

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binding magnesium ion: D84 (= D94), N112 (= N122), V114 (= V124)
binding iron/sulfur cluster: C8 (= C19), C11 (= C22), C42 (= C52), C183 (= C213), T185 (= T215)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (≠ V50), G41 (= G51), C42 (= C52), S43 (= S53), H59 (= H69), G85 (= G95), D86 (= D96), N112 (= N122), V114 (= V124), Y115 (≠ F125), G116 (= G126), L117 (≠ M127)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
27% identity, 91% coverage: 18:241/246 of query aligns to 4:226/275 of 5b47B

query
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5b47B

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binding magnesium ion: D83 (= D94), N111 (= N122)
binding iron/sulfur cluster: C5 (= C19), C8 (= C22), C39 (= C52), C179 (= C213)
binding thiamine diphosphate: I37 (≠ V50), G38 (= G51), C39 (= C52), S40 (= S53), H58 (= H69), G84 (= G95), D85 (= D96), N111 (= N122), V113 (= V124), Y114 (≠ F125), L116 (≠ M127)

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
30% identity, 86% coverage: 18:228/246 of query aligns to 11:213/305 of P72579

query
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P72579

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K49 (≠ F55) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (≠ M127) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
30% identity, 86% coverage: 18:228/246 of query aligns to 11:213/305 of Q96Y68

query
sites
Q96Y68

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C12 (= C19) binding [4Fe-4S] cluster
C15 (= C22) binding [4Fe-4S] cluster
C46 (= C52) binding [4Fe-4S] cluster
K49 (≠ F55) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D94) binding Mg(2+)
GD 91:92 (= GD 95:96) binding thiamine diphosphate
N118 (= N122) binding Mg(2+)
V120 (= V124) binding Mg(2+)
GL 122:123 (≠ GM 126:127) binding thiamine diphosphate
K125 (≠ G129) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (= K189) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C213) binding [4Fe-4S] cluster

9bt4C Pyruvate:ferredoxin oxidoreductase from methanosarcina acetivorans (see paper)
25% identity, 77% coverage: 28:217/246 of query aligns to 10:210/291 of 9bt4C

query
sites
9bt4C

H

H

R

R

L

G

T
x
C

A

A

E

G

C
|
C

I
x
C

D
|
D

R

A

Y

L

A

A

L

S

Q

K

-

F

-

M

-

L

-

M

-

G

-

A

-

G

E

P

T

D

S

T

I

I

A

V

V

I

A

N

S

P

V
x
T

G
|
G

C
|
C

-

L

-

E

-

V

-

M

-

T

S

T

V

P

F

F

L

P

Y

E

D

S

Y

A

L

W

D

Q

V

V

D

P

V

W

L

I

E

H

A

S

P

L

H
x
F

G
x
E

R

N

A

G

A

A

V

V

A

A

T

S

G

G

V

V

K

E

R

A

A

A

-

L

-

K

-

A

-

L

-

G

-

R

R

K

P

G

D

N

R

T

F

R

V

V

F

I

T

G

Y

V

Q

G

G
|
G

D
|
D

G
|
G

D
x
S

L

T

A

M

A

D

I

I

G

G

T

I

A

R

E

S

I

L

I

S

H

G

A

A

A

F

N

E

R

R

G

G

D

H

R

D

I

I

T

T

V

Y

I

V

F

C

V

V

N

D

N
|
N

T

E

V
x
A

F
x
Y

G
x
M

M
x
N

T
|
T

G

G

G

I

Q

Q

M

R

A

S

P

S

T

G

T

T

M

P

P

F

G

D

Q

A

E

S

T

T

T

S

P

P

F

A

G

G

R

K

D

V

P

S

A

F

V

G

N

N

G

P

F

R

P

P

I

K

R

K

M

D

A

M

E

P

L

A

L

I

G

M

G

A

L

A

T

H

G

G

T

S

T

P

F

Y

V

V

A

A

R

T

G

T

A

S

V

I

D

G

T

F

P

P

K

R

N

D

L

M

V

M

K

R

T

K

R

-

K

-

Y

-

M

V

Q

K

R

K

A

A

F

T

E

E

V

I

Q

V

T

-

G

-

E

-

G

G

F

P

G

T

F

Y

V

I

E

H

I

S

L

H

S

A

A

P

C
|
C

P

P

T
|
T

N

G

W

W

binding magnesium ion: D94 (= D94), N122 (= N122), A124 (≠ V124)
binding iron/sulfur cluster: C13 (≠ T31), C16 (= C34), C17 (≠ I35), D18 (= D36), C41 (= C52), M126 (≠ G126), C206 (= C213), T208 (= T215)
binding thiamine diphosphate: D18 (= D36), T39 (≠ V50), G40 (= G51), C41 (= C52), F63 (≠ H69), E64 (≠ G70), G93 (= G93), D94 (= D94), G95 (= G95), S96 (≠ D96), N122 (= N122), A124 (≠ V124), Y125 (≠ F125), M126 (≠ G126), N127 (≠ M127), T128 (= T128)

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
24% identity, 90% coverage: 8:229/246 of query aligns to 12:241/314 of 5exeC

query
sites
5exeC

P

P

Q

D

S

E

L

E

I

Y

D

Y

V

V

P

P

F

G

H

H

-

R

F

T

C
|
C

P

A

G

G

C
|
C

H

G

H
x
P

G

A

I

L

I

T

H

Y

R

R

L

L

T

V

A

A

E

K

C

A

I

A

D

G

R

P

Y

N

A

T

L

I

-

F

-

I

-

G

-

P

-
x
T

Q
x
G

E
x
C

T

M

S

Y

I

V

A

A

V

N

A

T

S

S

V

Y

G

G

C

C

S

G

V

P

F

W

L

R

Y

V

D

P

Y

W

L

I

D

H

V

A

D

Q

V
x
I

L

T

E

N

A

G

-

G

-

A

-

V

P

A

H

S

G

G

R

I

A

E

A

A

V

Y

A

K

T

A

G

M

V

I

K

R

R

K

A

K

R

K

P

T

D

D

R

A

-

E

-

F

-

P

F

N

V

I

F

I

T

V

Y

M

Q

A

G
|
G

D
|
D

G
|
G

D

G

L

A

A

V

A

D

I

I

G

G

T

L

A

Q

E

A

I

L

I

S

H

A

A

M

A

L

N

Y

R

R

G

G

D

H

R
x
D

I

V

T

L

V

F

I

I

F

C

V
x
Y

N

D

N
|
N

T

E

V
x
S

F
x
Y

G
x
A

M
x
N

T
|
T

G

G

G

I

Q

Q

M

T

A

S

P

P

T

T

M

P

P

Y

G

G

Q

A

E

N

T

T

T

F

T

T

P

P

F

P

G

G

R

E

-

V

D

P

P

E

A

G

V

K

N

K

G

L

F

F

P

P

I

K

R

D

M

N

A

P

E

K

L

V

L

I

G

A

-

H

G

G

L

H

T

P

G

E

T

L

T

K

F

Y

V

V

A

A

R

T

G

A

A

S

V

I

D

G

T

W

P

P

K

V

N

D

L

L

V

M

-

N

K

K

T

V

R

R

K

K

Y

G

M
x
L

Q

N

R
x
Q

A

-

F

-

E

-

V

-

Q

-

T

-

G

-

E

E

G

G

F

P

G

A

F

Y

V

I

E

H

I

I

L

H

S

A

A

P

C
|
C

P
|
P

T
x
K

N

G

W

W

K

Q

M

F

S

P

P

A

E

D

R

K

A

T

N

I

K

E

R

M

V

A

K

K

active site: N143 (≠ M127)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (vs. gap), G51 (≠ Q41), C52 (≠ E42), I74 (≠ V64), G109 (= G93), D110 (= D94), G111 (= G95), Y136 (≠ V120), N138 (= N122), S140 (≠ V124), Y141 (≠ F125), A142 (≠ G126), N143 (≠ M127), T144 (= T128)
binding magnesium ion: D110 (= D94), D130 (≠ R114), N138 (= N122), S140 (≠ V124), L211 (≠ M191), Q213 (≠ R193)
binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ H24), C52 (≠ E42), C225 (= C213), P226 (= P214), K227 (≠ T215)

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
24% identity, 90% coverage: 8:229/246 of query aligns to 12:241/310 of 5exdF

query
sites
5exdF

P

P

Q

D

S

E

L

E

I

Y

D

Y

V

V

P

P

F

G

H

H

-

R

F

T

C
|
C

P

A

G

G

C
|
C

H

G

H
x
P

G

A

I

L

I

T

H

Y

R

R

L

L

T

V

A

A

E

K

C

A

I

A

D

G

R

P

Y

N

A

T

L

I

-

F

-

I

-

G

-

P

-
x
T

Q
x
G

E
x
C

T

M

S

Y

I

V

A

A

V

N

A

T

S

S

V

Y

G

G

C

C

S

G

V

P

F

W

L

R

Y

V

D

P

Y

W

L

I

D

H

V

A

D

Q

V
x
I

L

T

E

N

A

G

-

G

-

A

-

V

P

A

H

S

G

G

R

I

A

E

A

A

V

Y

A

K

T

A

G

M

V

I

K

R

R

K

A

K

R

K

P

T

D

D

R

A

-

E

-

F

-

P

F

N

V

I

F

I

T

V

Y

M

Q

A

G

G

D
|
D

G
|
G

D

G

L

A

A

V

A

D

I

I

G

G

T

L

A

Q

E

A

I

L

I

S

H

A

A

M

A

L

N

Y

R

R

G

G

D

H

R

D

I

V

T

L

V

F

I

I

F

C

V
x
Y

N

D

N
|
N

T

E

V
x
S

F
x
Y

G
x
A

M
x
N

T
|
T

G

G

G

I

Q

Q

M

T

A

S

P

P

T

T

M

P

P

Y

G

G

Q

A

E

N

T

T

T

F

T

T

P

P

F

P

G

G

R

E

-

V

D

P

P

E

A

G

V

K

N

K

G

L

F

F

P

P

I

K

R

D

M

N

A

P

E

K

L

V

L

I

G

A

-

H

G

G

L

H

T

P

G

E

T

L

T

K

F

Y

V

V

A

A

R

T

G

A

A

S

V

I

D

G

T

W

P

P

K

V

N

D

L

L

V

M

-

N

K

K

T

V

R

R

K

K

Y

G

M

L

Q

N

R

Q

A

-

F

-

E

-

V

-

Q

-

T

-

G

-

E

E

G

G

F

P

G

A

F

Y

V

I

E

H

I

I

L

H

S

A

A

P

C
|
C

P

P

T
x
K

N

G

W

W

K

Q

M

F

S

P

P

A

E

D

R

K

A

T

N

I

K

E

R

M

V

A

K

K

active site: N143 (≠ M127)
binding magnesium ion: D110 (= D94), N138 (= N122), S140 (≠ V124)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (vs. gap), G51 (≠ Q41), C52 (≠ E42), I74 (≠ V64), D110 (= D94), G111 (= G95), Y136 (≠ V120), N138 (= N122), S140 (≠ V124), Y141 (≠ F125), A142 (≠ G126), N143 (≠ M127), T144 (= T128)
binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ H24), C52 (≠ E42), A142 (≠ G126), C225 (= C213), K227 (≠ T215)

5c4iC Structure of an oxalate oxidoreductase (see paper)
24% identity, 90% coverage: 8:229/246 of query aligns to 12:241/312 of 5c4iC

query
sites
5c4iC

P

P

Q

D

S

E

L

E

I

Y

D

Y

V

V

P

P

F

G

H

H

-

R

F

T

C
|
C

P

A

G

G

C
|
C

H

G

H
x
P

G

A

I

L

I

T

H

Y

R

R

L

L

T

V

A

A

E

K

C

A

I

A

D

G

R

P

Y

N

A

T

L

I

-

F

-

I

-

G

-

P

-
x
T

Q
x
G

E
x
C

T

M

S

Y

I

V

A

A

V

N

A

T

S

S

V

Y

G

G

C

C

S

G

V

P

F

W

L

R

Y

V

D

P

Y

W

L

I

D

H

V

A

D

Q

V
x
I

L

T

E

N

A

G

-

G

-

A

-

V

P

A

H

S

G

G

R

I

A

E

A

A

V

Y

A

K

T

A

G

M

V

I

K

R

R

K

A

K

R

K

P

T

D

D

R

A

-

E

-

F

-

P

F

N

V

I

F

I

T

V

Y

M

Q

A

G
|
G

D
|
D

G
|
G

D

G

L

A

A

V

A

D

I

I

G

G

T

L

A

Q

E

A

I

L

I

S

H

A

A

M

A

L

N

Y

R

R

G

G

D

H

R

D

I

V

T

L

V

F

I

I

F

C

V
x
Y

N

D

N
|
N

T

E

V
x
S

F
x
Y

G
x
A

M
x
N

T
|
T

G

G

G

I

Q

Q

M

T

A

S

P

P

T

T

M

P

P

Y

G

G

Q

A

E

N

T

T

T

F

T

T

P

P

F

P

G

G

R

E

-

V

D

P

P

E

A

G

V

K

N

K

G

L

F

F

P

P

I

K

R

D

M

N

A

P

E

K

L

V

L

I

G

A

-

H

G

G

L

H

T

P

G

E

T

L

T

K

F

Y

V

V

A

A

R

T

G

A

A

S

V

I

D

G

T

W

P

P

K

V

N

D

L

L

V

M

-

N

K

K

T

V

R

R

K

K

Y

G

M

L

Q

N

R

Q

A

-

F

-

E

-

V

-

Q

-

T

-

G

-

E

E

G

G

F

P

G

A

F

Y

V

I

E

H

I

I

L

H

S

A

A

P

C
|
C

P
|
P

T
x
K

N

G

W

W

K

Q

M

F

S

P

P

A

E

D

R

K

A

T

N

I

K

E

R

M

V

A

K

K

active site: N143 (≠ M127)
binding magnesium ion: D110 (= D94), N138 (= N122), S140 (≠ V124)
binding iron/sulfur cluster: C24 (= C19), C27 (= C22), P29 (≠ H24), C52 (≠ E42), A142 (≠ G126), C225 (= C213), P226 (= P214), K227 (≠ T215)
binding thiamine diphosphate: T50 (vs. gap), G51 (≠ Q41), C52 (≠ E42), I74 (≠ V64), G109 (= G93), D110 (= D94), G111 (= G95), Y136 (≠ V120), N138 (= N122), S140 (≠ V124), Y141 (≠ F125), A142 (≠ G126), N143 (≠ M127), T144 (= T128)

Query Sequence

>WP_028319867.1 NCBI__GCF_000422285.1:WP_028319867.1
MEQVFSRPQSLIDVPFHFCPGCHHGIIHRLTAECIDRYALQETSIAVASVGCSVFLYDYL
DVDVLEAPHGRAAAVATGVKRARPDRFVFTYQGDGDLAAIGTAEIIHAANRGDRITVIFV
NNTVFGMTGGQMAPTTMPGQETTTTPFGRDPAVNGFPIRMAELLGGLTGTTFVARGAVDT
PKNLVKTRKYMQRAFEVQTTGEGFGFVEILSACPTNWKMSPERANKRVKEEMIPYFPLGI
FKGGDA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory