SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028320056.1 NCBI__GCF_000422285.1:WP_028320056.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

6addA The crystal structure of rv2747 from mycobacterium tuberculosis in complex with coa and nlq (see paper)
36% identity, 84% coverage: 7:144/165 of query aligns to 6:134/168 of 6addA

query
sites
6addA

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binding coenzyme a: Y24 (= Y25), I28 (≠ L30), V75 (≠ L85), A76 (= A86), V77 (= V87), T82 (≠ R92), G83 (≠ E93), H84 (= H94), G85 (= G95), G87 (= G97), H88 (≠ L98), T110 (= T120), E112 (≠ V122), F115 (= F125)
binding n~2~-acetyl-l-glutamine: K27 (≠ Q28), I28 (≠ L30), L30 (= L32), T74 (≠ S84), L109 (= L119)

Sites not aligning to the query:

5yo2A The crystal structure of rv2747 from mycobacterium tuberculosis in complex with acetyl coa and l-arginine (see paper)
36% identity, 84% coverage: 7:144/165 of query aligns to 6:134/168 of 5yo2A

query
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5yo2A

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binding acetyl coenzyme *a: Y24 (= Y25), I28 (≠ L30), I72 (≠ V82), R73 (= R83), T74 (≠ S84), V75 (≠ L85), V77 (= V87), T82 (≠ R92), G83 (≠ E93), H84 (= H94), G85 (= G95), G87 (= G97), H88 (≠ L98), T110 (= T120), E112 (≠ V122), F115 (= F125), F116 (= F126)
binding arginine: G26 (≠ K27), L29 (= L31), L30 (= L32), R73 (= R83), L109 (= L119)

Sites not aligning to the query:

binding arginine: 147

5ygeA Arga complexed with acecoa and glutamate (see paper)
36% identity, 84% coverage: 7:144/165 of query aligns to 7:135/169 of 5ygeA

query
sites
5ygeA

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binding acetyl coenzyme *a: Y25 (= Y25), I73 (≠ V82), V76 (≠ L85), A77 (= A86), V78 (= V87), T83 (≠ R92), G84 (≠ E93), H85 (= H94), G86 (= G95), G88 (= G97), H89 (≠ L98), T111 (= T120), E113 (≠ V122), F117 (= F126)
binding glutamic acid: K33 (≠ R34), E44 (≠ D45), H64 (≠ G73), R74 (= R83)

Sites not aligning to the query:

binding acetyl coenzyme *a: 1, 2

4i49A Structure of ngnags bound with bisubstrate analog coa-NAG (see paper)
27% identity, 88% coverage: 2:146/165 of query aligns to 272:407/424 of 4i49A

query
sites
4i49A

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binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): I300 (≠ L30), L301 (= L31), L302 (= L32), R304 (= R34), A343 (≠ R83), C344 (≠ S84), L345 (= L85), A346 (= A86), V347 (= V87), Q352 (≠ R92), D353 (≠ E93), G355 (= G95), G357 (= G97), E358 (≠ L98), L379 (= L119), S380 (vs. gap), T383 (≠ V122), W386 (≠ F125), F387 (= F126)

Sites not aligning to the query:

binding (2S)-2-({(3S,5R,9R)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14,20-trioxo-2,4,6-trioxa-18-thia-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaicosan-20-yl}amino)pentanedioic acid (non-preferred name): 413, 415

3d2mA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-glutamate (see paper)
27% identity, 88% coverage: 2:146/165 of query aligns to 272:407/424 of 3d2mA

query
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3d2mA

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binding coenzyme a: L345 (= L85), V347 (= V87), Q352 (≠ R92), D353 (≠ E93), G355 (= G95), G357 (= G97), E358 (≠ L98), S380 (vs. gap), T383 (≠ V122), W386 (≠ F125)
binding glutamic acid: L302 (= L32), R304 (= R34), A343 (≠ R83), C344 (≠ S84), L379 (= L119)

Sites not aligning to the query:

active site: 26
binding glutamic acid: 413, 415

3b8gA Crysta structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and n-acetyl-glutamate (see paper)
27% identity, 88% coverage: 2:146/165 of query aligns to 272:407/424 of 3b8gA

query
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3b8gA

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binding coenzyme a: I300 (≠ L30), L345 (= L85), V347 (= V87), Q352 (≠ R92), D353 (≠ E93), G355 (= G95), Y356 (≠ L96), G357 (= G97), E358 (≠ L98), S380 (vs. gap), T383 (≠ V122), E385 (≠ D124), W386 (≠ F125)
binding n-acetyl-l-glutamate: L302 (= L32), R304 (= R34), A343 (≠ R83), C344 (≠ S84), L379 (= L119)

Sites not aligning to the query:

active site: 26
binding n-acetyl-l-glutamate: 413, 415

2r8vA Native structure of n-acetylglutamate synthase from neisseria gonorrhoeae (see paper)
27% identity, 88% coverage: 2:146/165 of query aligns to 272:407/424 of 2r8vA

query
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2r8vA

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A

K

E

I

R

G

G

F

F

Q

Q

V

T

V

A

D

S

K

E

A

D

V

E

L

L

P

P

H

E

K

T

V

R

W

R

A

K

D

D

binding acetyl coenzyme *a: I300 (≠ L30), L301 (= L31), L345 (= L85), V347 (= V87), Q352 (≠ R92), D353 (≠ E93), G355 (= G95), G357 (= G97), E358 (≠ L98), T383 (≠ V122), E385 (≠ D124), W386 (≠ F125), F387 (= F126)

Sites not aligning to the query:

active site: 26

P0A6C5 Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Escherichia coli (strain K12) (see paper)
25% identity, 85% coverage: 1:140/165 of query aligns to 292:422/443 of P0A6C5

query
sites
P0A6C5

M

M

E

E

T

S

D

A

M

E

H

Q

L

I

R

R

K

R

A

A

R

T

T

I

G

N

D

D

V

I

H

G

A

G

I

I

Y

L

D

E

I

L

L

I

T

R

H

P

Y

L

A

E

K

Q

Q

Q

G

G

L

I

L

L

L

V

Q

R

R

R

P

S

L

R

S

E

E

Q

L

L

Y

E

D

M

H

E

L

I

R

D

D

K

Y

F

V

T

V

I

L

I

E

Q

R

R

S

D

E

N

A

T

G

T

E

I

S

A

S

C

G

A

S

A

S

L

S

Y

G

P

F

F

E

P

V

-

V

-

G

-

V

-

C

-

G

-

L

-

G

-

V

-

C

-

W

E

E

E

D

K

L

I

A

G

E

E

V

M

R

A

S

C

L

V

A

A

V

V

L

H

E

P

A

D

Y

Y

R

R

E

S

H

S

G

S

L

R

G

G

L

E

K

V

L

L

V

L

E

E

A

R

C

I

L

A

E

A

E

Q

A

A

R

K

V

Q

L

S

G

G

L

L

K

S

R

K

V

L

F

F

T

V

L

L

T

T

Y

T

V

R

P

S

-

I

D

H

F

W

F

F

G

Q

K

E

I

R

G

G

F

F

Q

T

V

P

V

V

D

D

K

I

A

D

V

L

L

L

P

P

Sites not aligning to the query:

15 H→Y: In EE17.
19 Y→C: In EE51.
54 S→N: In PT2M217.
58 R→H: In EE11.
287 G→S: In PT2M216.
432 Q→R: In PT2M217.

3d2pA Crystal structure of n-acetylglutamate synthase from neisseria gonorrhoeae complexed with coenzyme a and l-arginine (see paper)
26% identity, 88% coverage: 2:146/165 of query aligns to 280:411/424 of 3d2pA

query
sites
3d2pA

E

E

T

A

D

F

M

V

H

S

L

I

R

R

K

Q

A

A

R

H

T

S

G

G

D

D

V

I

H

P

A

H

I

I

Y

A

D

A

I

L

L

I

T

-

H

-

Y

-

A

-

K

R

Q

P

G
x
L

L

I

L

L

L

L

Q

H

R

R

P

S

L

R

S

E

E

Y

L

L

Y

E

D

N

H

H

L

I

R

S

D

E

Y

F

V

S

V

I

L

L

E

E

R

H

S

D

E

G

A

-

G

-

E

-

S

-

S

-

G

-

S

-

S

-

S

-

G

-

F

-

E

N

V

L

V

Y

G

G

V

C

C

A

G

A

L

L

G

K

V

T

C

F

W

A

E

E

-

A

D

D

L

C

A

G

E

E

V

I

R

A

S

C

L
|
L

A

A

V
|
V

L

S

E

P

A

Q

Y

A

R
x
Q

E
x
D

H

G

G
|
G

L

Y

G
|
G

L
x
E

K

R

L

L

V

L

E

A

A

H

C

I

L

I

E

D

E

K

A

A

R

R

V

G

L

I

G

G

L

I

K

S

R

R

V

L

F

F

T

A

L

L

-

S

T

T

Y

N

V

T

P

G

D
x
E

F
x
W

F

F

G

A

K

E

I

R

G

G

F

F

Q

Q

V

T

V

A

D

S

K

E

A

D

V

E

L

L

P

P

H

E

K

T

V

R

W

R

A

K

D

D

binding coenzyme a: L303 (≠ G29), L349 (= L85), V351 (= V87), Q356 (≠ R92), D357 (≠ E93), G359 (= G95), G361 (= G97), E362 (≠ L98), E389 (≠ D124), W390 (≠ F125)

Sites not aligning to the query:

active site: 26
binding arginine: 13, 197, 216, 253, 266, 267, 269, 270, 271, 274, 276

Query Sequence

>WP_028320056.1 NCBI__GCF_000422285.1:WP_028320056.1
METDMHLRKARTGDVHAIYDILTHYAKQGLLLQRPLSELYDHLRDYVVLERSEAGESSGS
SSGFEVVGVCGLGVCWEDLAEVRSLAVLEAYREHGLGLKLVEACLEEARVLGLKRVFTLT
YVPDFFGKIGFQVVDKAVLPHKVWADCLKCPKFPDCDEIAMMRAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory