SitesBLAST
Comparing WP_028321334.1 NCBI__GCF_000422285.1:WP_028321334.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 12 hits to proteins with known functional sites (download)
4r1mA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.48 a resolution
30% identity, 98% coverage: 7:442/443 of query aligns to 6:435/435 of 4r1mA
- binding adenosine monophosphate: A215 (≠ G222), E216 (= E223), P217 (= P224), N236 (≠ D245), S237 (≠ H246), F238 (≠ A247), G239 (= G248), M240 (≠ A249), T241 (= T250), D305 (= D314), R329 (= R339), I335 (≠ K345), N340 (≠ L350)
- binding zinc ion: C252 (= C259), H259 (= H267), C314 (= C323), C316 (= C325)
4r1lA Crystal structure of a putative acyl-coa ligase (bt_0428) from bacteroides thetaiotaomicron vpi-5482 at 2.42 a resolution
30% identity, 98% coverage: 7:442/443 of query aligns to 6:433/433 of 4r1lA
- binding adenosine-5'-diphosphate: A215 (≠ G222), E216 (= E223), P217 (= P224), S237 (≠ H246), F238 (≠ A247), G239 (= G248), M240 (≠ A249), T241 (= T250), D305 (= D314), R329 (= R339), N340 (≠ L350)
- binding adenosine monophosphate: A215 (≠ G222), E216 (= E223), P217 (= P224), S237 (≠ H246), F238 (≠ A247), G239 (= G248), M240 (≠ A249), T241 (= T250), D305 (= D314), R329 (= R339), N340 (≠ L350)
- binding coenzyme a: S136 (≠ P143), A164 (≠ V171), G165 (≠ L172), N166 (≠ D173), S167 (≠ T174), I185 (≠ T192), Y188 (= Y195), K337 (= K347), T408 (≠ R415)
- binding zinc ion: C252 (= C259), H259 (= H267), C314 (= C323), C316 (= C325)
6he0A Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in complex with 2-hib-amp and coa in the thioesterfication state (see paper)
29% identity, 86% coverage: 7:388/443 of query aligns to 24:409/477 of 6he0A
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] 2-methyl-2-oxidanyl-propanoate: S241 (≠ A221), G242 (= G222), E243 (= E223), P244 (= P224), G267 (≠ A247), S268 (≠ G248), M269 (≠ A249), A270 (≠ T250), D335 (= D314), I357 (≠ V336), N371 (≠ L350)
- binding adenosine monophosphate: G242 (= G222), E243 (= E223), P244 (= P224), C266 (≠ H246), G267 (≠ A247), S268 (≠ G248), A270 (≠ T250), E271 (= E251), D335 (= D314), N371 (≠ L350)
- binding coenzyme a: Y166 (≠ F149), A188 (≠ G169), G189 (= G170), P191 (≠ L172), S194 (≠ K175), Y210 (≠ M190), G211 (≠ A191), T212 (= T192), Y215 (= Y195), H218 (≠ G198), R368 (≠ K347), G369 (= G348), M401 (≠ S380)
Sites not aligning to the query:
2y27B Crystal structure of paak1 in complex with atp from burkholderia cenocepacia (see paper)
32% identity, 94% coverage: 5:419/443 of query aligns to 1:396/427 of 2y27B
- binding adenosine-5'-triphosphate: K65 (= K70), S90 (≠ T101), S91 (= S102), G92 (= G103), T93 (= T104), T94 (= T105), F138 (= F149), A211 (≠ G222), E212 (= E223), P213 (= P224), D232 (= D245), I233 (≠ H246), Y234 (≠ A247), G235 (= G248), L236 (≠ A249), S237 (≠ T250), D302 (= D314), I320 (≠ V336), R323 (= R339)
- binding magnesium ion: V200 (≠ A211), S202 (≠ L213), L204 (≠ I215), M226 (≠ W239), G227 (= G240), Q347 (≠ I363), L350 (= L366)
Sites not aligning to the query:
2y4oA Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
31% identity, 99% coverage: 4:441/443 of query aligns to 1:433/433 of 2y4oA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (= F144), F140 (= F149), A213 (≠ G222), E214 (= E223), P215 (= P224), I235 (≠ H246), G237 (= G248), L238 (≠ A249), S239 (≠ T250), P244 (≠ G253), D304 (= D314), R325 (= R339), I331 (≠ K345), N336 (≠ L350)
2y4oB Crystal structure of paak2 in complex with phenylacetyl adenylate (see paper)
31% identity, 99% coverage: 4:440/443 of query aligns to 1:432/432 of 2y4oB
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: F135 (= F144), F140 (= F149), G212 (≠ A221), A213 (≠ G222), E214 (= E223), P215 (= P224), I235 (≠ H246), G237 (= G248), L238 (≠ A249), S239 (≠ T250), P244 (≠ G253), D304 (= D314), R325 (= R339), I331 (≠ K345), N336 (≠ L350)
- binding magnesium ion: S204 (≠ L213), V228 (≠ W239)
6hdyA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with s3-hb-amp (see paper)
27% identity, 98% coverage: 7:441/443 of query aligns to 24:462/474 of 6hdyA
- binding (3s)-3-hydroxybutanoic acid: Y162 (≠ F149), S237 (≠ A221), G263 (≠ A247), S264 (≠ G248), M265 (≠ A249), A266 (≠ T250), F271 (≠ W255)
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (3~{S})-3-oxidanylbutanoate: Y162 (≠ F149), G164 (≠ A151), S237 (≠ A221), G238 (= G222), E239 (= E223), P240 (= P224), C262 (≠ H246), G263 (≠ A247), S264 (≠ G248), A266 (≠ T250), F271 (≠ W255), D331 (= D314), I353 (≠ V336), R356 (= R339), K453 (= K432)
6hdxA Crystal structure of 2-hydroxyisobutyryl-coa ligase (hcl) in the postadenylation state in complex with r3-hib-amp (see paper)
27% identity, 98% coverage: 7:441/443 of query aligns to 24:462/474 of 6hdxA
- binding [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (2~{R})-2-methyl-3-oxidanyl-propanoate: Y162 (≠ F149), G164 (≠ A151), S237 (≠ A221), G238 (= G222), E239 (= E223), P240 (= P224), C262 (≠ H246), G263 (≠ A247), S264 (≠ G248), A266 (≠ T250), F271 (≠ W255), D331 (= D314), I353 (≠ V336), R356 (= R339), K453 (= K432)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: Y162 (≠ F149), G164 (≠ A151), S237 (≠ A221), G263 (≠ A247), S264 (≠ G248), A266 (≠ T250), F271 (≠ W255)
2y4nA Paak1 in complex with phenylacetyl adenylate (see paper)
31% identity, 94% coverage: 5:419/443 of query aligns to 1:394/426 of 2y4nA
- binding 5'-o-[hydroxy(phenylacetyl)phosphoryl]adenosine: Y131 (≠ F144), F136 (= F149), G138 (≠ A151), G208 (≠ A221), A209 (≠ G222), E210 (= E223), P211 (= P224), I231 (≠ H246), Y232 (≠ A247), G233 (= G248), L234 (≠ A249), S235 (≠ T250), P240 (≠ G253), D300 (= D314), R321 (= R339)
- binding magnesium ion: V198 (≠ A211), S200 (≠ L213), Q345 (≠ I363), L348 (= L366)
Sites not aligning to the query:
6sixB Paak family amp-ligase with anp (see paper)
29% identity, 80% coverage: 19:373/443 of query aligns to 17:354/437 of 6sixB
- binding phosphoaminophosphonic acid-adenylate ester: S88 (≠ T101), S89 (= S102), A213 (≠ G222), E214 (= E223), P215 (= P224), E236 (≠ H246), Y237 (≠ A247), G238 (= G248), S239 (≠ A249), T240 (= T250), E241 (= E251), D300 (= D314), V320 (= V336), R323 (= R339)
- binding magnesium ion: R79 (≠ L92), E80 (= E93), P121 (= P136), T150 (≠ E165)
- binding zinc ion: C249 (= C259), H255 (≠ G265), C309 (= C323), C311 (= C325)
6siyA Paak family amp-ligase with amp and substrate (see paper)
29% identity, 80% coverage: 19:373/443 of query aligns to 13:350/433 of 6siyA
- binding 3-hydroxyanthranilic acid: T125 (≠ F144), P126 (≠ G145), T132 (≠ A151), L135 (≠ A154), R153 (vs. gap), N177 (≠ T192), A209 (≠ G222), E232 (≠ H246), G234 (= G248), S235 (≠ A249)
- binding adenosine monophosphate: S85 (= S102), A209 (≠ G222), E210 (= E223), P211 (= P224), E232 (≠ H246), Y233 (≠ A247), G234 (= G248), S235 (≠ A249), T236 (= T250), D296 (= D314), V316 (= V336)
- binding magnesium ion: R75 (≠ L92), E76 (= E93), L78 (≠ V95), P117 (= P136), G144 (= G163), A145 (≠ C164), T146 (≠ E165)
6siwA Paak family amp-ligase with amp (see paper)
29% identity, 80% coverage: 19:373/443 of query aligns to 12:349/432 of 6siwA
- binding adenosine monophosphate: S84 (= S102), A208 (≠ G222), E209 (= E223), P210 (= P224), E231 (≠ H246), Y232 (≠ A247), G233 (= G248), S234 (≠ A249), T235 (= T250), D295 (= D314), V315 (= V336)
- binding magnesium ion: E75 (= E93), L77 (≠ V95), S83 (≠ T101), P116 (= P136), G143 (= G163), T145 (≠ E165), E236 (= E251)
- binding zinc ion: C244 (= C259), H250 (≠ G265), C304 (= C323), C306 (= C325)
Query Sequence
>WP_028321334.1 NCBI__GCF_000422285.1:WP_028321334.1
MVDHPIYWNPVIETLPRQKLRALQLKKFKRIFAWTYDHSRFHRALYDQAGVRPEDIRTFE
DIRAVPKVEKSMMRDIQRKDPFPYGDALCVPLEEVTEFRQTSGTTGQPVYQPDTWQDWEW
WAECWACILWAQGYRPSDRVFLPFGYNIFVAFWAGHYAAEKIGCEVVPGGVLDTKARILK
MQELRATAMMATPTYVLGMADTARKMGIDPAALSIRRITCAGEPGAGIPSTKKRMQDAWG
AKVFDHAGATEIGAWSFECAEQPFGMHVNEGMFLVEIEDIETGEIIEEPGRRGKMVITAF
DRQAQPCVRFDSKDVIEWDSEPCRCGRTFRLIKGGVVGRADDITKVKGVLLAPSAIEEVV
RSIEGLGDEYEVIVDKVGDSDRISLKVELLPEARGNRKAVEAALVDQLRLKTNLRYDLEF
NDYGQLPRYEVKAKRFKDLRKDH
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory