SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028321394.1 NCBI__GCF_000422285.1:WP_028321394.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
40% identity, 87% coverage: 23:493/540 of query aligns to 28:523/557 of P78753

query
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P78753

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S391 (≠ I367) modified: Phosphoserine
S489 (= S459) modified: Phosphoserine

P22106 Asparagine synthetase B [glutamine-hydrolyzing]; AS-B; EC 6.3.5.4 from Escherichia coli (strain K12) (see 2 papers)
38% identity, 91% coverage: 1:492/540 of query aligns to 1:507/554 of P22106

query
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P22106

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M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H25) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D29) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ F76) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H100) mutation to H: Little effect on the kinetic properties.
E349 (= E333) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
39% identity, 88% coverage: 23:495/540 of query aligns to 27:490/497 of 1ct9A

query
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1ct9A

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P

D

G

A

-

S

-

G

-

D

-

V

-

S

S

D

P

D
|
D

V

L

K

K

A

A

K

Q

I

E

A

V

A

A

A

N

H

H

I

L

G

G

S

T

T

V

H

H

E

E

L

I

L

H

F

F

T

T

E

V

A

Q

E

E

Y

G

H

L

E

D

A

A

L

I

P

R

L

D

V

V

I

I

Q

Y

K

H

L

I

E

E

T

T

Y

Y

D

D

P

V

S
x
T

L

T

V

I

R
|
R

C

A

A

S

V

T

P

P

C

M

Y

Y

F

L

T
x
M

C

S

-

R

K

K

L

I

A

K

A

A

R

M

Y

G

V

I

T

K

V

M

V

V

L

L

T
x
S

G
|
G

E
|
E

G

G

A

S

D

D

E

E

L

V

F

F

T

G

G

G

Y

Y

H

L

Y

Y

M

F

K

H

H

K

F

A

P

P

F

N

D

A

K

K

-

E

L

L

N

H

L

E

E

E

A

T

R

V

R

R

C

K

I

L

G

L

N

A

L

L

H

H

N
x
M

I

Y

N

D

L

C

Q

A

R

R

A

A

D

N

R

K

M

A

G

M

M

S

H

A

F

W

N

G

L

V

E

E

L

A

R

R

V

V

P

P

F

F

L

L

D

D

V

K

D

K

M

F

I

L

D

D

L

V

S

A

M

M

K

R

I

I

P

N

A

P

E

Q

L

D

K

K

I

M

R

-

E

-

H

-

G

-

A

C

K

K

I

M

E

E

K

K

W

H

I

I

L

L

R

R

M

E

A

C

F

F

E

E

D

-

T

-

D

A

Y

Y

L

L

P

P

D

A

D

S

I

V

L

A

W

W

R

R

Y

Q

K

K

V

E

Q

Q

Y

F

T

S

Q

D

G

G

A

V

G

G

C

Y

E

S

S

W

L

I

G

D

-

T

-

L

E

K

Q

E

M

V

A

A

E

A

A

Q

M

V

S

S

D

D

E

Q

Y

L

L

E

Q

T

I

A

R

R

A

F

E

R

N

F

P

P

E

Y

A

N

V

T

I

P

S

T

S

S

K

K

E

E

A

A

A

Y

Y

L

Y

Y

F

R

K

E

I

I

F

F

R

E

E

E

F

L

H

F

P

P

Q

L

D

P

S

S

active site: L50 (= L46), N74 (= N70), G75 (= G71), T305 (≠ S307), R308 (= R310), E332 (= E333), M366 (≠ N366)
binding adenosine monophosphate: L232 (≠ F227), L233 (≠ C228), S234 (= S229), S239 (= S234), A255 (≠ V252), V256 (= V253), D263 (= D265), M316 (≠ T318), S330 (≠ T331), G331 (= G332), E332 (= E333)
binding glutamine: R49 (= R45), L50 (= L46), I52 (= I48), V53 (= V49), N74 (= N70), G75 (= G71), E76 (= E72), D98 (= D94)

Sites not aligning to the query:

active site: 1
binding glutamine: 1

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
35% identity, 91% coverage: 1:492/540 of query aligns to 1:527/561 of P08243

query
sites
P08243

M

M

C
|
C

G

G

I

I

A

W

G
x
A

C

L

I

F

G

G

T

S

T

D

D

D

T

C

E

L

T

S

V

V

N

-

R

Q

M

C

L

L

D

S

A

A

L

M

-

K

-

I

G

A

H

H

R

R

G

G

P

P

D

D

D

A

-

F

-

R

-

F

R

E

G

N

I

V

H

N

P

G

R

Y

E

T

N

N

L

C

V

C

F

F

G

G

H

F

T

H

R

R

L

L

S

A

I

V

V

V

D

D

V

P

A

L

K

F

G

G

H

M

Q

Q

P

P

I

I

L

R

A

V

N

K

G

K

G

Y

S

P

A

Y

G

L

I

W

I

L

C

C

-

Y

N

N

G

G

E

E

I

I

Y

Y

N

N

F

H

R

K

Q

K

L

M

K

Q

H

Q

S

H

L

F

R

-

P

-

K

E

H

F

S

E

F

Y

K

Q

T

T

D

K

S

V

D

D

T

G

E

E

V

I

I

I

L

L

H

H

L

L

Y

Y

Q

D

E

K

K

G

G

G

P

I

E

E

-

Q

C

T

V

I

K

C

E

M

L

L

D

D

G

G

M

V

F

F

A

A

F

F

A

V

L

L

F

L

D

D

-

T

-

A

N

N

D

K

A

K

F

V

L

F

M

L

A

G

R

R

D

D

P

T

I

Y

G

G

I

V

K

R

P

P

L

L

Y

F

Y

K

G

A

Y

M

R

T

Q

E

-

D

G

G

A

F

L

L

Y

A

F

V

T

C

S

S

E

E

L

A

-

K

G

G

A

L

M

V

T

T

L

L

-

K

-

H

-

S

-

A

A

T

G

P

V

F

D

L

E

K

V

V

H

E

E

P

F

F

P

L

A

P

G

G

H

H

Y

Y

-

E

-

V

-

L

-

D

Y

L

T

K

P

P

E

N

S

G

G

K

-

V

-

A

-

S

-

V

-

E

F

M

V

V

R

K

Y

Y

Y

H

D

H

I

C

P

R

P

D

I
x
V

E

P

D

L

H

H

L

A

L

L

T

Y

D

D

-

N

-

V

-

E

-

K

-

L

-

F

-

P

-

G

-

F

-

E

I

I

E

E

P

T

V

V

C

K

R

N

I

L

R

R

E

I

T

L

F

F

V

N

R

N

A

A

V

V

K

K

R

R

L

L

L

M

A

T

D

D

S

R

E

R

V

I

P

-

V

-

G

G

S

C

F

L

C

L

S

S

G

G

L

L

D

D

S

S

L

L

V

V

A

A

I

T

A

L

E

K

E

Q

I

L

P

K

N

E

-

A

-

Q

-

V

-

Q

-

Y

-

P

L

L

H

Q

T

T

F

F

V

A

V

I

G

G

M

M

K

E

D

D

A

-

S

-

G

-

D

-

V

-

S

S

D

P

D

D

V

L

K

L

A

A

K

R

I

K

A

V

A

A

A

D

H

H

I

I

G

G

S

S

T

E

H

H

H

Y

E

E

L

V

L

L

F

F

T

N

E

S

A

E

E

E

Y

G

H

I

E

Q

A

A

L

L

P

D

L

E

V

V

I

I

Q

F

K

S

L

L

E

E

T

T

Y

Y

D

D

P

I

S

T

L

T

V

V

R

R

C

A

A

S

V

V

P

G

C

M

Y

Y

F

L

T

I

C

S

K

K

L

Y

A

I

A

R

R

K

Y

N

V

T

-

D

-

S

T

V

V

V

V

I

L
x
F

T

S

G

G

E

E

G

G

A

S

D

D

E

E

L

L

F

T

T

Q

G

G

Y

Y

H

I

Y

Y

M

F

K

H

H

K

F

A

P

P

F

S

-

P

D

E

K

K

L

A

N

E

L

E

E

E

A

S

R

E

R

R

C

L

I

L

G

R

N

E

L

L

H

Y

N

L

I

F

N

D

L

V

Q

L

R

R

A

A

D

D

R

R

M

T

G

T

M

A

H

A

F

H

N

G

L

L

E

E

L

L

R

R

V

V

P

P

F

F

L

L

D

D

V

H

D

R

M

F

I

S

D

S

L

Y

S

Y

M

L

K

S

I

L

P

P

A

P

E

E

L

M

K

R

I

I

R

P

E

K

H

N

H

-

G

-

A

-

K

G

I

I

E

E

K

K

W

H

I

L

L

L

R

R

M

E

A

T

F

F

E

E

D

D

T

S

D

N

Y

L

L

I

P

P

D

K

D

E

I

I

L

L

W

W

R

R

Y

P

K

K

V

E

Q

A

Y

F

T

S

Q

D

G

G

A

I

-

T

-

S

-

V

-

K

-

N

G

S

C

W

E

F

S

K

L

I

G

L

E

Q

Q

E

M

Y

A

V

E

E

A

H

A

Q

M

V

S

D

D

D

E

A

E

M

Y

M

L

A

Q

N

I

A

R

A

A

Q

E

K

N

F

P

P

E

F

A

N

V

T

I

P

S

K

S

T

K

K

E

E

A

G

A

Y

Y

Y

F

R

K

Q

I

V

F

F

R

E

E

R

F

H

H

Y

P

P

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ I189) to E: in dbSNP:rs1049674
F362 (≠ L330) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
34% identity, 91% coverage: 2:492/540 of query aligns to 1:501/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

I

I

A
x
W

G

A

C

L

I

F

G

G

T

S

T

D

D

D

T

C

E

L

T

S

V

V

N

-

R

Q

M

C

L

L

D

S

A

A

L

M

-

K

-

I

G

A

H

H

R

R

G

G

P

P

D

D

D

A

-

F

-

R

-

F

R

E

G

N

I

V

H

N

P

G

R

Y

E

T

N

N

L

C

V

C

F

F

G

G

H

F

T

H

R
|
R

L
|
L

S

A

I
x
V

V
|
V

D

D

V

P

A

L

K

F

G

G

H

M

Q

Q

P

P

I

I

L

R

A

V

N

K

G

K

G

Y

S

P

A

Y

G

L

I

W

I

L

C

C

-
x
Y

N
|
N

G
|
G

E
|
E

I

I

Y

Y

N

N

F

H

R

K

Q

K

L

M

K

Q

H

Q

S

H

L

F

R

-

P

-

K

E

H

F

S

E

F

Y

K

Q

T

T

D

K

S
x
V

D
|
D

T

G

E

E

V

I

I

I

L

L

H

H

L

L

Y

Y

Q

D

E

K

K

G

G

G

P

I

E

E

-

Q

C

T

V

I

K

C

E

M

L

L

D

D

G

G

M

V

F

F

A

A

F

F

A

V

L

L

F

L

D

D

-

T

-

A

N

N

D

K

A

K

F

V

L

F

M

L

A

G

R

R

D

D

P

T

I

Y

G

G

I

V

K

R

P

P

L

L

Y

F

Y

K

G

A

Y

M

R

T

Q

E

-

D

G

G

A

F

L

L

Y

A

F

V

T

C

S

S

E

E

L

A

-

K

G

G

A

L

M

V

T

T

L

L

-

K

-

H

-

S

-

A

A

T

G

P

V

F

D

L

E

K

V

V

H

E

E

P

F

F

P

L

A

P

G

G

H

H

Y

Y

-

E

-

V

-

L

-

D

-

L

-

K

-

P

-

N

-

G

Y

K

T

V

P

A

E

S

S

V

G

E

F

M

V

V

R

K

Y

Y

Y

H

D

H

I

C

P

R

P

D

I

V

E

F

D

P

H

G

L

F

L

-

T

-

D

E

I

I

E

E

P

T

V

V

C

K

R

N

I

L

R

R

E

I

T

L

F

F

V

N

R

N

A

A

V

V

K

K

R

R

L

L

L

M

A

T

D

D

S

R

E

R

V

I

P

-

V

-

G

G

S

C

F

L

C

L

S

S

G

G

L

L

D

D

S

S

L

L

V

V

A

A

I

T

A

L

E

K

E

Q

I

L

P

K

N

E

-

A

-

Q

-

V

-

Q

-

Y

-

P

L

L

H

Q

T

T

F

F

V

A

V

I

G

G

M

M

K

E

D

D

A

-

S

-

G

-

D

-

V

-

S

S

D

P

D

D

V

L

K

L

A

A

K

R

I

K

A

V

A

A

A

D

H

H

I

I

G

G

S

S

T

E

H

H

H

Y

E

E

L

V

L

L

F

F

T

N

E

S

A

E

E

E

Y

G

H

I

E

Q

A

A

L

L

P

D

L

E

V

V

I

I

Q

F

K

S

L

L

E

E

T

T

Y

Y

D

D

P

I

S
x
T

L

T

V

V

R
|
R

C

A

A

S

V

V

P

G

C

M

Y

Y

F

L

T

I

C

S

K

K

L

Y

A

I

A

R

R

K

Y

N

V

T

-

D

-

S

T

V

V

V

V

I

L

F

T

S

G

G

E

E

G

G

A

S

D

D

E

E

L

L

F

T

T

Q

G

G

Y

Y

H

I

Y

Y

M

F

K

H

H

K

F

A

P

P

F

S

-

P

D

E

K

K

L

A

N

E

L

E

E

E

A

S

R

E

R

R

C

L

I

L

G

R

N

E

L

L

H

Y

N

L

I

F

N

D

L

V

Q

L

R

R

A

A

D

D

R

R

M

T

G

T

M

A

H

A

F

H

N

G

L

L

E

E

L

L

R

R

V

V

P

P

F

F

L

L

D

D

V

H

D

R

M

F

I

S

D

S

L

Y

S

Y

M

L

K

S

I

L

P

P

A

P

E

E

L

M

K

R

I

I

R

P

E

K

H

N

H

-

G

-

A

-

K

G

I

I

E

E

K

K

W

H

I

L

L

L

R

R

M

E

A

T

F

F

E

E

D

D

T

S

D

N

Y

L

L

I

P

P

D

K

D

E

I

I

L

L

W

W

R

R

Y

-

K

-

V

-

Q

-

Y

-

T

-

Q

-

G

-

A

P

G

S

C

W

E

F

S

K

L

I

G

L

E

Q

Q

E

M

Y

A

V

E

E

A

H

A

Q

M

V

S

D

D

D

E

A

E

M

Y

M

L

A

Q

N

I

A

R

A

A

Q

E

K

N

F

P

P

E

F

A

N

V

T

I

P

S

K

S

T

K

K

E

E

A

G

A

Y

Y

Y

F

R

K

Q

I

V

F

F

R

E

E

R

F

H

H

Y

P

P

active site: W4 (≠ A5), L49 (= L46), N74 (= N70), G75 (= G71), T324 (≠ S307), R327 (= R310)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R45), V51 (≠ I48), V52 (= V49), Y73 (vs. gap), N74 (= N70), G75 (= G71), E76 (= E72), V95 (≠ S93), D96 (= D94)

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
26% identity, 72% coverage: 61:448/540 of query aligns to 56:438/491 of 1mc1A

query
sites
1mc1A

N

T

G

G

G

A

S

P

A

T

G

T

I

A

I

V

C

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

R

D

Q

E

L

L

K

L

H

S

S

V

L

L

R

-

P

-

K

P

H

A

S

G

F

P

K

A

T

P

D

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

H

R

L

L

Y

L

Q

E

E

R

K

Y

G

D

P

L

E

H

C

A

V

F

K

R

E

L

L

V

D

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

F

R

D

T

N

G

D

D

A

R

F

V

L

L

M

L

A

A

R

T

D

D

P

H

I

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

G

C

Y

V

R

A

Q

P

G

G

A

E

L

V

Y

R

F

A

T

S

S

T

E

E

L

A

G

K

A

A

M

L

T

A

-

A

-

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

L

V

A

A

G

G

V

L

D

T

E

G

V

V

H

Y

E

Q

F

V

P

P

A

A

G

G

H

A

Y

V

Y

M

T

D

P

I

E

D

S

L

G

G

-

S

-

G

-

T

-

A

F

V

V

T

R

H

Y

R

Y

T

D

W

I

T

P

P

P

G

I

L

E

S

D

R

H

R

L

I

L

L

T

P

D

E

I

G

E

E

P

A

V

V

C

A

R

A

R

-

I

V

R

R

E

A

T

A

F

L

V

E

R

K

A

A

V

V

K

A

K

Q

R

R

L

V

L

-

A

T

D

P

S

G

E

D

V

T

P

P

V

L

G

-

S

V

F
x
V

C

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

V

V

A

A

I

C

A

A

A

H

E

R

E

A

I

A

P

G

N

E

L

L

H

D

T

T

F

V

V
x
S

V
x
M

G

G

M

T

K

-

D

-

A

-

S

-

G

-

D

D

V

T

S

S

D

N

D

E

V

F

K

R

A

E

A

A

K

R

I

A

A

V

A

D

H

H

I

L

G

R

S

T

T

R

H

H

H

R

E

E

L

I

L

T

F

I

T

P

E

T

A

T

E

E

Y

L

H

L

E

A

A

Q

L

L

P

P

L

Y

V

A

I

V

Q

W

K

A

L

S

E

E

T

S

Y

V

D

D

P

P

S
x
D

L

I

V

I

R

E

C
x
Y

A

L

V

L

P

P

C

L

Y

T

F

A

T

L

C

Y

K

R

L

A

A

L

A

D

R

G

Y

P

V

E

T

R

V

R

V

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

L

P

F

L

T

G

G

G

Y

M

H

H

Y

R

M

E

K

D

H

R

F

L

P

P

-

A

F

L

D

D

K

T

L

V

N

L

L

A

E

H

A
x
D

R

M

R

A

C

T

I

F

G

D

N

G

L

L

H

N

N
x
E

I

M

N

S

L

P

Q

V

R

L

A

S

D

T

R

L

M

A

G

G

M

-

H

H

F

W

N

T

L

T

E

H

L

-

R

-

V

-

P

P

F

Y

L

W

D

D

V

R

D

E

M

V

I

L

D

D

L

L

S

L

M

V

K

S

I

L

P

E

A

A

E

G

L

L

K

K

I

R

R

R

E

-

H

-

H

H

G

G

A

R

K

-

I

-

E

D

K
|
K

W

W

I

V

L

L

R

R

M

A

A

A

F

M

E

A

D

D

T

A

D

-

Y

-

L

L

P

P

D

A

D

E

I

T

L

V

W

N

R

R

Y

P

K
|
K

V
x
L

Q

G

Y
x
V

T

H

Q

E

G

G

A

S

G

G

C

T

E

T

S

S

active site: A65 (≠ N70), G66 (= G71), D306 (≠ S307), Y332 (≠ E333), E366 (≠ N366), K427 (= K437)
binding adenosine monophosphate: V231 (≠ F227), S233 (= S229), S238 (= S234), S256 (≠ V252), M257 (≠ V253), G331 (= G332), K427 (= K437), V430 (≠ Y440)
binding magnesium ion: D237 (= D233), D335 (= D336)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ C311), Y332 (≠ E333), G333 (= G334), I336 (≠ E337), D357 (≠ A357), E366 (≠ N366), K427 (= K437)
binding pyrophosphate 2-: S233 (= S229), G235 (= G231), D237 (= D233), S238 (= S234), D335 (= D336), K407 (= K415), K427 (= K437), L428 (≠ V438)

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
26% identity, 72% coverage: 61:448/540 of query aligns to 60:443/496 of 1mbzA

query
sites
1mbzA

N

T

G

G

G

A

S

P

A

T

G

T

I

A

I

V

C

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

R

D

Q

E

L

L

K

L

H

S

S

V

L

L

R

-

P

-

K

P

H

A

S

G

F

P

K

A

T

P

D

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

H

R

L

L

Y

L

Q

E

E

R

K

Y

G

D

P

L

E

H

C

A

V

F

K

R

E

L

L

V

D

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

F

R

D

T

N

G

D

D

A

R

F

V

L

L

M

L

A

A

R

T

D

D

P

H

I

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

G

C

Y

V

R

A

Q

P

G

G

A

E

L

V

Y

R

F

A

T

S

S

T

E

E

L

A

G

K

A

A

M

L

T

A

-

A

-

H

-

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

L

V

A

A

G

G

V

L

D

T

E

G

V

V

H

Y

E

Q

F

V

P

P

A

A

G

G

H

A

Y

V

Y

M

T

D

P

I

E

D

S

L

G

G

-

S

-

G

-

T

-

A

F

V

V

T

R

H

Y

R

Y

T

D

W

I

T

P

P

P

G

I

L

E

S

D

R

H

R

L

I

L

L

T

P

D

E

I

G

E

E

P

A

V

V

C

A

R

A

R

-

I

V

R

R

E

A

T

A

F

L

V

E

R

K

A

A

V

V

K

A

K

Q

R

R

L

V

L

-

A

T

D

P

S

G

E

D

V

T

P

P

V

L

G

-

S

V

F
x
V

C
x
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

V

V

A

A

I

C

A

A

A

H

E

R

E

A

I

A

P

G

N

E

L

L

H

D

T

T

F

V

V
x
S

V
x
M

G

G

M

T

K

-

D

-

A

-

S

-

G

-

D

D

V

T

S

S

D

N

D

E

V

F

K

R

A

E

A

A

K

R

I

A

A

V

A

D

H

H

I

L

G

R

S

T

T

R

H

H

H

R

E

E

L

I

L

T

F

I

T

P

E

T

A

T

E

E

Y

L

H

L

E

A

A

Q

L

L

P

P

L

Y

V

A

I

V

Q

W

K

A

L

S

E

E

T

S

Y

V

D

D

P

P

S
x
D

L

I

V

I

R

E

C
x
Y

A

L

V

L

P

P

C
x
L

Y

T

F

A

T

L

C

Y

K

R

L

A

A

L

A

D

R

G

Y

P

V

E

T

R

V

R

V

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

L

P

F

L

T

G

G

G

Y

M

H

H

Y

R

M

E

K

D

H

R

F

L

P

P

-

A

F

L

D

D

K

T

L

V

N

L

L

A

E

H

A
x
D

R

M

R

A

C

T

I

F

G

D

N

G

L

L

H

N

N
x
E

I

M

N

S

L

P

Q

V

R

L

A

S

D

T

R

L

M

A

G

G

M

-

H

H

F

W

N

T

L

T

E

H

L

-

R

-

V

-

P

P

F

Y

L

W

D

D

V

R

D

E

M

V

I

L

D

D

L

L

S

L

M

V

K

S

I

L

P

E

A

A

E

G

L

L

K

K

I

R

R

R

E

-

H

-

H

H

G

G

A

R

K

-

I

-

E

D

K
|
K

W

W

I

V

L

L

R

R

M

A

A

A

F

M

E

A

D

D

T

A

D

-

Y

-

L

L

P

P

D

A

D

E

I

T

L

V

W

N

R

R

Y

P

K
|
K

V
x
L

Q
x
G

Y
x
V

T

H

Q

E

G

G

A

S

G

G

C

T

E

T

S

S

active site: A69 (≠ N70), G70 (= G71), D311 (≠ S307), Y337 (≠ E333), E371 (≠ N366), K432 (= K437)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ F227), L237 (≠ C228), S238 (= S229), S243 (= S234), S261 (≠ V252), M262 (≠ V253), Y315 (≠ C311), L319 (≠ C315), G336 (= G332), Y337 (≠ E333), G338 (= G334), D340 (= D336), I341 (≠ E337), D362 (≠ A357), E371 (≠ N366), K432 (= K437), G434 (≠ Q439), V435 (≠ Y440)
binding magnesium ion: D242 (= D233), D340 (= D336)
binding pyrophosphate 2-: S238 (= S229), G240 (= G231), D242 (= D233), S243 (= S234), D340 (= D336), K412 (= K415), K432 (= K437), L433 (≠ V438)

1mb9A Beta-lactam synthetase complexed with atp (see paper)
26% identity, 71% coverage: 61:442/540 of query aligns to 61:436/485 of 1mb9A

query
sites
1mb9A

N

T

G

G

G

A

S

P

A

T

G

T

I

A

I

V

C

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

R

D

Q

E

L

L

K

L

H

S

S

V

L

L

R

-

P

-

K

P

H

A

S

G

F

P

K

A

T

P

D

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

H

R

L

L

Y

L

Q

E

E

R

K

Y

G

D

P

L

E

H

C

A

V

F

K

R

E

L

L

V

D

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

F

R

D

T

N

G

D

D

A

R

F

V

L

L

M

L

A

A

R

T

D

D

P

H

I

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

G

C

Y

V

R

A

Q

P

G

G

A

E

L

V

Y

R

F

A

T

S

S

T

E

E

L

A

G

K

A

A

M

L

T

A

-

A

-

F

-

P

-

L

-

A

-

D

-

A

-

R

L

V

A

A

G

G

V

L

D

T

E

G

V

V

H

Y

E

Q

F

V

P

P

A

A

G

G

H

A

Y

V

Y

M

T

D

P

I

E

D

S

L

G

G

-

S

-

G

-

T

-

A

F

V

V

T

R

H

Y

R

Y

T

D

W

I

T

P

P

P

G

I

L

E

S

D

R

H

R

L

I

L

L

T

P

D

E

I

G

E

E

P

A

V

V

C

A

R

A

R

-

I

V

R

R

E

A

T

A

F

L

V

E

R

K

A

A

V

V

K

A

K

Q

R

R

L

V

L

-

A

T

D

P

S

G

E

D

V

T

P

P

V

L

G

-

S

V

F
x
V

C
x
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

V

V

A

A

I

C

A

A

A

H

E

R

E

A

I

A

P

G

N

E

L

L

H

D

T

T

F

V

V
x
S

V
x
M

G

G

M

T

K

-

D

-

A

-

S

-

G

-

D

D

V

T

S

S

D

N

D

E

V

F

K

R

A

E

A

A

K

R

I

A

A

V

A

D

H

H

I

L

G

R

S

T

T

R

H

H

H

R

E

E

L

I

L

T

F

I

T

P

E

T

A

T

E

E

Y

L

H

L

E

A

A

Q

L

L

P

P

L

Y

V

A

I

V

Q

W

K

A

L

S

E

E

T

S

Y

V

D

D

P

P

S
x
D

L

I

V

I

R

E

C
x
Y

A

L

V

L

P

P

C
x
L

Y

T

F

A

T

L

C

Y

K

R

L

A

A

L

A

D

R

G

Y

P

V

E

T

R

V

R

V

I

L

L

T

T

G
|
G

E
x
Y

G

G

A

A

D
|
D

E

I

L

P

F

L

T

G

G

G

Y

M

H

H

Y

R

M

E

K

D

H

R

F

L

P

P

-

A

F

L

D

D

K

T

L

V

N

L

L

A

E

H

A

D

R

M

R

A

C

T

I

F

G

D

N

G

L

L

H

N

N
x
E

I

M

N

S

L

P

Q

V

R

L

A

S

D

T

R

L

M

A

G

G

M

-

H

H

F

W

N

T

L

T

E

H

L

-

R

-

V

-

P

P

F

Y

L

W

D

D

V

R

D

E

M

V

I

L

D

D

L

L

S

L

M

V

K

S

I

L

P

E

A

A

E

G

L

L

K

K

I

R

R

R

E

-

H

-

H

H

G

G

A

R

K

-

I

-

E

D

K
|
K

W

W

I

V

L

L

R

R

M

A

A

A

F

M

E

A

D

D

T

A

D

-

Y

-

L

L

P

P

D

A

D

E

I

T

L

V

W

N

R

R

Y

P

K
|
K

V

S

Q

S

Y

F

T

S

Q

R

active site: A70 (≠ N70), G71 (= G71), D310 (≠ S307), Y336 (≠ E333), E370 (≠ N366), K431 (= K437)
binding adenosine monophosphate: V235 (≠ F227), L236 (≠ C228), S242 (= S234), S260 (≠ V252), M261 (≠ V253), Y314 (≠ C311), L318 (≠ C315), G335 (= G332), Y336 (≠ E333)
binding adenosine-5'-triphosphate: V235 (≠ F227), L236 (≠ C228), S237 (= S229), G239 (= G231), D241 (= D233), S242 (= S234), S260 (≠ V252), M261 (≠ V253), L318 (≠ C315), G335 (= G332), D339 (= D336), K411 (= K415), K431 (= K437)
binding magnesium ion: D241 (= D233), D339 (= D336)
binding pyrophosphate 2-: S237 (= S229), G239 (= G231), D241 (= D233), S242 (= S234), D339 (= D336), K411 (= K415), K431 (= K437)

1jgtB Crystal structure of beta-lactam synthetase (see paper)
25% identity, 71% coverage: 61:441/540 of query aligns to 64:444/500 of 1jgtB

query
sites
1jgtB

N

T

G

G

G

A

S

P

A

T

G

T

I

A

I

V

C

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

R

D

Q

E

L

L

K

L

H

S

S

V

L

L

R

-

P

-

K

P

H

A

S

G

F

P

K

A

T

P

D

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

H

R

L

L

Y

L

Q

E

E

R

K

Y

G

D

P

L

E

H

C

A

V

F

K

R

E

L

L

V

D

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

F

R

D

T

N

G

D

D

A

R

F

V

L

L

M

L

A

A

R

T

D

D

P

H

I

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

G

C

Y

V

R

A

Q

P

G

G

A

E

L

V

Y

R

F

A

T

S

S

T

E

E

L

A

G

K

A

A

M

L

T

A

-

A

-

H

-

R

-

D

-

P

-

K

-

G

-

F

-

P

-

L

-

A

-

D

-

A

-

R

L

V

A

A

G

G

V

L

D

T

E

G

V

V

H

Y

E

Q

F

V

P

P

A

A

G

G

H

A

Y

V

Y

M

T

D

P

I

E

D

S

L

G

G

-

S

-

G

-

T

-

A

F

V

V

T

R

H

Y

R

Y

T

D

W

I

T

P

P

P

G

I

L

E

S

D

R

H

R

L

I

L

L

T

P

D

E

I

G

E

E

P

A

V

V

C

A

R

A

R

-

I

V

R

R

E

A

T

A

F

L

V

E

R

K

A

A

V

V

K

A

K

Q

R

R

L

V

L

-

A

T

D

P

S

G

E

D

V

T

P

P

V

L

G

-

S

V

F
x
V

C
x
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

S

S

L

G

V

V

A

A

I

C

A

A

A

H

E

R

E

A

I

A

P

G

N

E

L

L

H

D

T

T

F

V

V
x
S

V
x
M

G

G

M

T

K

-

D

-

A

-

S

-

G

-

D

D

V

T

S

S

D

N

D

E

V

F

K

R

A

E

A

A

K

R

I

A

A

V

A

D

H

H

I

L

G

R

S

T

T

R

H

H

H

R

E

E

L

I

L

T

F

I

T

P

E

T

A

T

E

E

Y

L

H

L

E

A

A

Q

L

L

P

P

L

Y

V

A

I

V

Q

W

K

A

L

S

E

E

T

S

Y

V

D

D

P

P

S
x
D

L

I

V

I

R

E

C
x
Y

A

L

V

L

P

P

C
x
L

Y

T

F

A

T

L

C

Y

K

R

L

A

A

L

A

D

R

G

Y

P

V

E

T

R

V

R

V

I

L

L

T

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

L

P

F

L

T

G

G

G

Y
x
M

H

H

Y

R

M

E

K

D

H

R

F

L

P

P

-

A

F

L

D

D

K

T

L

V

N

L

L

A

E

H

A
x
D

R

M

R

A

C

T

I

F

G

D

N

G

L

L

H

N

N
x
E

I

M

N

S

L

P

Q

V

R

L

A

S

D

T

R

L

M

A

G

G

M

-

H

H

F

W

N

T

L

T

E

H

L

-

R

-

V

-

P

P

F

Y

L

W

D

D

V

R

D

E

M

V

I

L

D

D

L

L

S

L

M

V

K

S

I

L

P

E

A

A

E

G

L

L

K

K

I

R

R

R

E

-

H

-

H

H

G

G

A

R

K

-

I

-

E

D

K
|
K

W

W

I

V

L

L

R

R

M

A

A

A

F

M

E

A

D

D

T

A

D

-

Y

-

L

L

P

P

D

A

D

E

I

T

L

V

W

N

R

R

Y

P

K
|
K

V

L

Q

S

Y

G

T

T

active site: A73 (≠ N70), G74 (= G71), D319 (≠ S307), Y345 (≠ E333), E379 (≠ N366), K440 (= K437)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ F227), L245 (≠ C228), S246 (= S229), G248 (= G231), I249 (≠ L232), D250 (= D233), S251 (= S234), S269 (≠ V252), M270 (≠ V253), L327 (≠ C315), G344 (= G332), Y345 (≠ E333), D348 (= D336), K420 (= K415), K440 (= K437)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ C311), Y345 (≠ E333), G346 (= G334), D348 (= D336), I349 (≠ E337), M354 (≠ Y342), D370 (≠ A357), E379 (≠ N366)
binding magnesium ion: D250 (= D233), D348 (= D336)

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
29% identity, 29% coverage: 2:159/540 of query aligns to 1:201/460 of 6lbpA

query
sites
6lbpA

C
|
C

G

G

I

V

A

V

G

G

C

I

I

Y

G

G

T

D

T

S

D

E

T

A

E

S

T

R

V

L

N

C

R

Y

M

L

-

A

L

L

D

H

A

A

L

L

G

Q

H

H

R

R

G
|
G

P

Q

D

E

D

G

R

A

G

G

I

I

H

V

P

T

R

V

E

S

-

K

-

D

-

K

-

V

-

L

-

Q

-

T

-

I

-

T

-

G

-

V

-

G

-

L

-

V

N

S

L

E

V

V

F

F

G

S

H

E

T

S

R

K

L

L

S

D

I

Q

V

L

-

P

-

G

D

D

V

I

A

A

K

I

G

G

H

H

-

V

-

R

-

Y

-

S

-

T

-

A

-

G

-

S

-

M

-

L

-

K

-

N

-

V

Q

Q

P

P

I

F

L

V

A

A

N

G

-

Y

-

R

G

F

G

G

S

S

A

V

G

G

I

V

I

A

C

H

N
|
N

G
|
G

E

N

I

L

Y

V

N

N

F

Y

R

T

Q

K

L

L

K

R

H

A

S

D

L

L

R

E

P

E

K

N

H

G

S

S

-

I

F

F

K

N

T

T

D

S

S

S

D

D

T

T

E

E

V

V

I

V

L

L

H

H

L

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

-

P

Y

F

Q

F

E

M

K

R

G

I

P

V

E

D

C

A

V

C

K

E

E

K

L

L

D

Q

G

G

M

A

F

Y

A

S

F

M

A

V

L

F

F

V

D

T

N

E

D

D

A

K

F

L

L

V

M

A

A

V

R

R

D

D

P

P

I

H

G

G

I

F

K

R

P

P

L

L

Y

V

Y

M

G

G

Y

R

R

R

-

S

Q

N

G

G

A

A

L

V

Y

V

F

F

T

A

S

S

E

E

L

T

G

C

A

A

M

L

T

D

L

L

active site: C1 (= C2), G27 (= G27), N102 (= N70), G103 (= G71)

Sites not aligning to the query:

active site: 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 29% coverage: 2:159/540 of query aligns to 87:287/561 of Q9STG9

query
sites
Q9STG9

C

C

G

G

I

V

A

V

G

G

C

I

I

Y

G

G

T

D

T

S

D

E

T

A

E

S

T

R

V

L

N

C

R

Y

M

L

-

A

L

L

D

H

A

A

L

L

G

Q

H

H

R

R

G

G

P

Q

D

E

D

G

R

A

G

G

I

I

H

V

P

T

R

V

E

S

-

K

-

D

-

K

-

V

-

L

-

Q

-

T

-

I

-

T

-

G

-

V

-

G

-

L

-

V

N

S

L

E

V

V

F

F

G

S

H

E

T

S

R

K

L

L

S

D

I

Q

V

L

-

P

-

G

D

D

V

I

A

A

K

I

G

G

H

H

-

V

-

R

-

Y

-

S

-

T

-

A

-

G

-

S

-

M

-

L

-

K

-

N

-

V

Q

Q

P

P

I

F