SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028486633.1 NCBI__GCF_000483485.1:WP_028486633.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3slhD 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
54% identity, 97% coverage: 10:441/446 of query aligns to 9:432/440 of 3slhD

query
sites
3slhD

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active site: K23 (= K24), S24 (= S25), D50 (= D51), N95 (= N95), R125 (= R125), D317 (= D324), E345 (= E352), H388 (= H397), R389 (= R398), T415 (= T424)
binding glyphosate: K23 (= K24), G97 (= G97), T98 (= T98), R125 (= R125), Q171 (= Q173), D317 (= D324), E345 (= E352), R348 (= R355), H388 (= H397), R389 (= R398)
binding shikimate-3-phosphate: S24 (= S25), R28 (= R29), S169 (= S171), Q171 (= Q173), R196 (= R198), D317 (= D324), K344 (= K351)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S24 (= S25), R28 (= R29), T98 (= T98), Q171 (= Q173), R196 (= R198), D317 (= D324), K344 (= K351)

Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
54% identity, 97% coverage: 10:441/446 of query aligns to 7:430/438 of Q83E11

query
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Q83E11

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K21 (= K24) binding phosphoenolpyruvate
S22 (= S25) binding 3-phosphoshikimate
R26 (= R29) binding 3-phosphoshikimate
NSGT 93:96 (= NSGT 95:98) Phosphoenolpyruvate
G95 (= G97) binding phosphoenolpyruvate
T96 (= T98) binding phosphoenolpyruvate
R123 (= R125) binding phosphoenolpyruvate
S167 (= S171) binding 3-phosphoshikimate
A168 (= A172) binding 3-phosphoshikimate
Q169 (= Q173) binding 3-phosphoshikimate; binding phosphoenolpyruvate
D315 (= D324) binding 3-phosphoshikimate
K342 (= K351) binding 3-phosphoshikimate
R346 (= R355) binding phosphoenolpyruvate
R387 (= R398) binding phosphoenolpyruvate

4egrA 2.50 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with phosphoenolpyruvate
54% identity, 97% coverage: 10:441/446 of query aligns to 9:428/434 of 4egrA

query
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4egrA

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V

I

I

I

N

Q

G

G

Q

-

S

T

P

L

Q

E

A

G

E

E

I

V

Q

N

S

S

H

Y

H

D

D

D

H
|
H

R
|
R

I

I

S

A

M

M

A

A

M

F

T

A

V

V

A

A

G

G

L

T

N

L

A

A

V

K

S

G

E

P

V

V

V

R

I

I

D

R

D

N

C

C

A

D

N

N

V

V

N

K

T
|
T

S

S

F

F

P

P

T

N

F

F

V

V

S

E

L

L

M

A

N

N

E

E

V

V

G

G

M

M

Q

N

I

V

E

K

active site: K23 (= K24), S24 (= S25), D50 (= D51), N95 (= N95), R125 (= R125), D313 (= D324), E341 (= E352), H384 (= H397), R385 (= R398), T411 (= T424)
binding phosphoenolpyruvate: K23 (= K24), G97 (= G97), T98 (= T98), R125 (= R125), D313 (= D324), E341 (= E352), R344 (= R355), R385 (= R398)

Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
46% identity, 93% coverage: 14:429/446 of query aligns to 10:417/427 of Q9S400

query
sites
Q9S400

I

L

H

H

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G

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I

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R

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P

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G

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D

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S

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H

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R

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I

I

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I

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F

G

G

A

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I

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A

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E

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Y

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D

A

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A

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Q

A

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D

M

L

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G

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E

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I

E

E

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D

D

K

-

G

G

N

V

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I

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Q

G

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V

G

G

L

M

R

A

G

G

L

L

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K

K

A

P

P

E

Q

K

N

P

A

M

L

D

N

M

M

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G

N

N

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G

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L

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A

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A

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L

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P

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D

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V

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T

D

L

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P

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M

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G

A

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A

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L

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P

P

M

L

K

R

I

L

C

K

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G

-

Q

K

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N

L

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I

I

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H

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Y

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E

L

L

P

P

M

I

A

A

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|
S

A
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A

Q
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Q

V

V

K

K

S

S

C

A

V

L

L

M

L

F

A

A

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A

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A

A

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C

V

A

I

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I

E

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K

A

E

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Y

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N

H

H

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E

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D

M

M

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L

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F

F

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Y

-

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-

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N

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D

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A

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G

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I

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E

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R

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T

G

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I

I

D

D

I

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R

L

A

M

M

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I

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E

A

I

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A

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K

E

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P

-

V

-

A

A

D

T

V

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H

I

I

V

R

E

Y

S

A

S

P

D

L

L

Q

K

G

G

I

T

E

E

I

I

P

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E

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A

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I

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A

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D
|
D

E

E

F

L

P

P

V

I

L

I

F

A

V

L

A

L

A

A

A

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Q

A

A

T

Q

G

G

Q

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T

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V

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D

A

A

E

E

L

L

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|
K

E

E

S

T

D

D

R

R

I

I

Q

Q

V

V

M

V

A

A

D

D

A

A

L

L

Q

N

A

S

V

M

G

G

I

A

D

D

A

I

V

T

P

P

T

T

P

A

D

D

G

G

M

M

V

I

I

I

N

K

G

-

G

G

Q

K

Q

S

S

A

P

L

Q

H

A

G

A

A

E

R

I

V

Q

N

S

T

H

F

H

G

D

D

H

H

R

R

I

I

S

G

M

M

A

M

M

T

T

A

V

I

A

A

G

A

-

L

L

L

N

V

A

A

V

D

S

G

E

E

V

V

V

E

I

L

D

D

D

R

C

A

A

E

N

A

V

I

N

N

T

T

S

S

F

Y

P

P

T

S

F

F

S21 (= S25) binding 3-phosphoshikimate
R25 (= R29) binding 3-phosphoshikimate
S166 (= S171) binding 3-phosphoshikimate
A167 (= A172) binding 3-phosphoshikimate
Q168 (= Q173) binding 3-phosphoshikimate
D312 (= D324) binding 3-phosphoshikimate
K339 (= K351) binding 3-phosphoshikimate

1rf6A Structural studies of streptococcus pneumoniae epsp synthase in s3p- glp bound state (see paper)
46% identity, 93% coverage: 14:429/446 of query aligns to 10:417/427 of 1rf6A

query
sites
1rf6A

I

L

H

H

G

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I

I

I

R

R

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P

G

G

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S

I

I

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H

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|
R

S

S

I

I

M

I

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G

A

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I

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A

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E

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E

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|
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D

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I

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E

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-

G

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G

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M

R

A

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G

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L

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K

K

A

P

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E

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N

P

A

M

L

D

N

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M

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G

N
|
N

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G
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G

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|
T

S

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I

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R

L

L

L

I

A

S

G

G

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L

A

A

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Q

A

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F

F

D

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C

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E

E

L

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F

G

G

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D

A
x
D

S

S

L

L

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K

R
|
R

P

P

M

M

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D

R

R

V

V

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T

D

L

P

P

L

L

A

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M

K

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M

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G

A

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A

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E

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D

T

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L

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I

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H

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Y

V

E

L

L

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|
S

A

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|
Q

V

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K

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C

A

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L

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A

A

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A

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A

A

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C

V

A

I

E

I

E

E

P

K

A

E

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|
R

D

N

H

H

T

T

E

E

R

D

M

M

L

L

N

Q

G

Q

F

F

G

G

Y

-

E

-

V

-

S

-

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G

R

H

L

L

D

S

N

V

S

D

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G

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R

K

A

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Q

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L

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D

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A

A

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F

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M

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A

A

A

G

A

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A

E

P

G

N

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R

L

L

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I

L

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N

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V

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G

M

I

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N

P

E

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R

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T

G

G

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I

I

I

D

D

I

V

L

I

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R

L

A

M

M

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G

A

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N

K

I

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E

L

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N

E

A

I

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V

G

A

G

K

E

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P

-

V

-

A

A

D

T

V

L

H

I

I

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R

E

Y

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A

S

P

D

L

L

Q

K

G

G

I

T

E

E

I

I

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C

E

G

S

A

L

L

V

I

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P

L

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A

L

I
|
I

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|
D

E

E

F

L

P

P

V

I

L

I

F

A

V

L

A

L

A

A

A

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S

Q

A

A

T

Q

G

G

Q

V

T

T

V

V

L

I

S

K

G

D

A

A

E

E

L

L

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K

V

V

K
|
K

E
|
E

S

T

D

D

R
|
R

I

I

Q

Q

V

V

M

V

A

A

D

D

A

A

L

L

Q

N

A

S

V

M

G

G

I

A

D

D

A

I

V

T

P

P

T

T

P

A

D

D

G

G

M

M

V

I

I

I

N

K

G

-

G

G

Q

K

Q

S

S

A

P

L

Q

H

A

G

A

A

E

R

I

V

Q

N

S

T

H

F

H

G

D

D

H
|
H

R
|
R

I

I

S

G

M

M

A

M

M

T

T

A

V

I

A

A

G

A

-

L

L

L

N

V

A

A

V

D

S

G

E

E

V

V

V

E

I

L

D

D

D

R

C

A

A

E

N

A

V

I

N

N

T
|
T

S

S

F

Y

P

P

T

S

F

F

active site: K20 (= K24), S21 (= S25), D47 (= D51), N90 (= N95), D115 (≠ A120), R120 (= R125), D312 (= D324), E340 (= E352), H384 (= H397), R385 (= R398), T412 (= T424)
binding glyphosate: K20 (= K24), G92 (= G97), T93 (= T98), R120 (= R125), Q168 (= Q173), D312 (= D324), E340 (= E352), R343 (= R355), H384 (= H397), R385 (= R398)
binding shikimate-3-phosphate: S21 (= S25), R25 (= R29), S166 (= S171), Q168 (= Q173), R193 (= R198), I311 (= I323), D312 (= D324), K339 (= K351)

1rf4A Structural studies of streptococcus pneumoniae epsp synthase, tetrahedral intermediate bound state (see paper)
46% identity, 93% coverage: 14:429/446 of query aligns to 10:417/427 of 1rf4A

query
sites
1rf4A

I

L

H

H

G

G

R

I

I

I

R

R

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V

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P

G

G

D

D

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S
|
S

I

I

S

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R

S

S

I

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I

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G

A

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G

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D

A

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D

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G

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M

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A

G

G

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L

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K

K

A

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N

P

A

M

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N

M

M

G

G

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|
N

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G

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|
T

S

S

T

I

R

R

L

L

L

I

A

S

G

G

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L

L

A

A

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G

Q

A

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D

F

F

D

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C

V

E

E

L

M

V

F

G

G

D

D

A
x
D

S

S

L

L

S

S

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K

R
|
R

P

P

M

M

K

D

R

R

V

V

T

T

D

L

P

P

L

L

A

K

M

K

M

M

G

G

A

V

K

S

I

I

E

S

-

G

T

Q

A

T

E

E

G

R

G

D

T

L

L

P

P

P

M

L

K

R

I

L

C

K

G

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T

G

-

Q

K

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N

L

L

K

R

A

P

I

I

D

H

Y

Y

V

E

L

L

P

P

M

I

A

A

S
|
S

A
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A

Q
|
Q

V

V

K

K

S

S

C

A

V

L

L

M

L

F

A

A

G

A

L

L

Y

Q

A

A

Q

K

G

G

K

E

T

S

C

V

A

I

E

I

E

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P

K

A

E

P

Y

T

T

R
|
R

D

N

H

H

T

T

E

E

R

D

M

M

L

L

N

Q

G

Q

F

F

G

G

Y

-

E

-

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-

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-

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L

D

S

N

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G

T

K

R

K

A

I

C

T

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Q

Q

G

G

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G

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K

L

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A

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K

I

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D

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V

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P

S

G

D

D

I

I

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S

A

A

F

F

F

W

M

L

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V

A

A

A

G

A

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I

S

A

E

P

G

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S

S

D

R

L

L

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I

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H

N

V

V

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G

M

I

N

N

P

E

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R

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T

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G

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I

I

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I

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R

L

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M

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K

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E

A

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A

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-

A

A

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D

L

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G

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E

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S

A

L

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P

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A

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D
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D

E

E

F

L

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V

I

L

I

F

A

V

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A

L

A

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A

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G

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A

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E

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D

D

R
|
R

I

I

Q

Q

V

V

M

V

A

A

D

D

A

A

L

L

Q

N

A

S

V

M

G

G

I

A

D

D

A

I

V

T

P

P

T

T

P

A

D

D

G

G

M

M

V

I

I

I

N

K

G

-

G

G

Q

K

Q

S

S

A

P

L

Q

H

A

G

A

A

E

R

I

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Q

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S

T

H

F

H

G

D

D

H
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H

R
|
R

I

I

S

G

M

M

A

M

M

T

T

A

V

I

A

A

G

A

-

L

L

L

N

V

A

A

V

D

S

G

E

E

V

V

V

E

I

L

D

D

D

R

C

A

A

E

N

A

V

I

N

N

T
|
T

S

S

F

Y

P

P

T

S

F

F

active site: K20 (= K24), S21 (= S25), D47 (= D51), N90 (= N95), D115 (≠ A120), R120 (= R125), D312 (= D324), E340 (= E352), H384 (= H397), R385 (= R398), T412 (= T424)
binding (3r,4s,5r)-5-{[(1r)-1-carboxy-2-fluoro-1-(phosphonooxy)ethyl]oxy}-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K20 (= K24), S21 (= S25), R25 (= R29), G92 (= G97), T93 (= T98), R120 (= R125), S166 (= S171), A167 (= A172), Q168 (= Q173), R193 (= R198), D312 (= D324), K339 (= K351), E340 (= E352), R343 (= R355), H384 (= H397), R385 (= R398)

Q9R4E4 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; CP4 EPSP synthase; EC 2.5.1.19 from Agrobacterium sp. (strain CP4) (see paper)
47% identity, 96% coverage: 14:441/446 of query aligns to 18:448/455 of Q9R4E4

query
sites
Q9R4E4

I

L

H

S

G

G

R

T

I

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R

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P

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G

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R

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S

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M

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F

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G

A

G

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A

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G

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G

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L

E

E

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G

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A

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A

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A

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M

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G

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A

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I

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K

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K

A

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P

E

E

K

A

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L

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F

G

G

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N

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A

G
x
A

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L

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A

M

G

G

I

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Y

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Y

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Q

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M

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-

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I

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A

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G

-

R

-

P

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D

-

G

-

K

G

G

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A

G

A

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H

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D

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A

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A

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M

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D

C

A

A

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M

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A

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S

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F

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E

F

F

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E

S29 (= S25) binding 3-phosphoshikimate
R33 (= R29) binding 3-phosphoshikimate
A100 (≠ G97) mutation to G: Confers sensitivity to glyphosate allowing glyphosate to bind in its extended, inhibitory conformation.
S173 (= S171) binding 3-phosphoshikimate
A174 (= A172) binding 3-phosphoshikimate
Q175 (= Q173) binding 3-phosphoshikimate
D326 (= D324) binding 3-phosphoshikimate
K353 (= K351) binding 3-phosphoshikimate

2pqcA Cp4 epsps liganded with (r)-phosphonate tetrahedral reaction intermediate analog (see paper)
47% identity, 96% coverage: 14:441/446 of query aligns to 13:443/445 of 2pqcA

query
sites
2pqcA

I

L

H

S

G

G

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S

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A

G

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A

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G

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T

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T

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G

F

L

L

L

E

E

G

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A

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D
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D

A

V

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I

A

N

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T

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A

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A

Q

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G

-

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G

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A

P

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E

E

K

A

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G

G

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N

S

A

G

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T

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G

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C

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R

L

L

L

T

A

M

G

G

I

L

L

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A

G

G

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Q

Y

D

D

F

F

D

D

C

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E

T

L

F

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G

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D

A

A

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K

R
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R

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P

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M

K

G

R

R

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N

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P

L

L

A

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M

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G

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A

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T

A

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Y

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M

A

A

S
|
S

A

A

Q
|
Q

V

V

K

K

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S

C

A

V

V

L

L

A

A

G

G

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Y

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A

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D

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M

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A

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D

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-

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A

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A

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D

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E

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P

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L

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A

V

V

A

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F

A

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G

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E

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K

E
|
E

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R

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A

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R

-

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-

D

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A

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A

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A

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H
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H

R
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R

I

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M

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A

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M

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M

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G

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D

C

A

A

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S

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F

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active site: K23 (= K24), S24 (= S25), D50 (= D51), N93 (= N95), R123 (= R125), D321 (= D324), E349 (= E352), H399 (= H397), R400 (= R398), T426 (= T424)
binding [3r-[3a,4a,5b(r*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K23 (= K24), S24 (= S25), R28 (= R29), T96 (= T98), R123 (= R125), S168 (= S171), Q170 (= Q173), D321 (= D324), K348 (= K351), E349 (= E352), R352 (= R355), R400 (= R398)

2pqbA Cp4 epsps liganded with (r)-difluoromethyl tetrahedral intermediate analog (see paper)
47% identity, 96% coverage: 14:441/446 of query aligns to 13:443/445 of 2pqbA

query
sites
2pqbA

I

L

H

S

G

G

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G

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S

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G

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G

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D

A

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A

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M

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G

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A

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E

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A

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D

D

M

F

G

G

N
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N

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A

G
x
A

T
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T

S

G

T

C

R

R

L

L

L

T

A

M

G

G

I

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A

G

G

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Q

Y

D

D

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F

D

D

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F

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G

D

D

A

A

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R
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R

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P

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M

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G

R

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D

N

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P

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A

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M

M

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G

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P

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M

A

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Q

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K

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S

C

A

V

V

L

L

A

A

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G

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K

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M

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T

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D

H

H

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Q

G

G

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F

G

G

Y

A

E

N

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L

-

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V

S

E

T

T

R

D

L

A

D

D

N

G

S

V

R

R

T

T

R

-

A

I

C

R

L

L

Q

E

G

G

G

R

G

G

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L

L

K

T

A

G

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H

V

I

I

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D

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G

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D

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S

A

T

A

A

F

F

F

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M

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A

A

A

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P

G

G

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S

D

D

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I

I

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N

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M

N

N

P

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T

G

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I

I

D

L

I

T

L

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K

Q

L

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M

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G

A

A

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D

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I

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E

L

V

E

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N

N

A

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G

G

E

E

P

D

V

V

A

A

D

D

V

L

H

R

I

V

R

R

Y

S

A

S

P

T

L

L

Q

K

G

G

I

V

E

T

I

V

P

P

E

E

S

D

L

R

V

A

P

P

L

S

A

M

I

I

D
|
D

E

E

F

Y

P

P

V

I

L

L

F

A

V

V

A

A

S

F

A

A

T

E

G

G

Q

A

T

T

V

V

L

M

S

N

G

G

A

L

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

Q

S

V

A

M

V

A

A

D

N

A

G

L

L

Q

K

A

L

V

N

G

G

I

V

D

D

A

C

V

D

P

E

T

G

P

E

D

T

G

S

M

L

V

V

I

V

N

R

G

G

-

R

-

P

-

D

-

G

-

K

G

G

Q

L

Q

G

S

N

P

A

Q

S

A

G

A

A

E

A

I

V

Q

A

S

T

H

H

H

L

D

D

H
|
H

R
|
R

I

I

S

A

M

M

A

S

M

F

T

L

V

V

A

M

G

G

L

L

N

V

A

S

V

E

S

N

E

P

V

V

V

T

I

V

D

D

C

A

A

T

N

M

V

I

N

A

T
|
T

S

S

F

F

P

P

T

E

F

F

V

M

S

D

L

L

M

M

N

A

E

G

V

L

G

G

M

A

Q

K

I

I

E

E

active site: K23 (= K24), S24 (= S25), D50 (= D51), N93 (= N95), R123 (= R125), D321 (= D324), E349 (= E352), H399 (= H397), R400 (= R398), T426 (= T424)
binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K23 (= K24), S24 (= S25), R28 (= R29), A95 (≠ G97), T96 (= T98), R123 (= R125), S168 (= S171), Q170 (= Q173), D321 (= D324), K348 (= K351), E349 (= E352), R352 (= R355), R400 (= R398)

2ggaA Cp4 epsp synthase liganded with s3p and glyphosate (see paper)
47% identity, 96% coverage: 14:441/446 of query aligns to 13:443/445 of 2ggaA

query
sites
2ggaA

I

L

H

S

G

G

R

T

I

V

R

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

I

F

M

M

L

F

G

G

A

G

I

L

S

A

E

S

G

G

T

E

T

T

T

R

V

I

T

T

G

G

F

L

L

L

E

E

G

G

A

E

D
|
D

A

V

L

I

A

N

T

T

L

G

Q

K

A

A

F

M

K

Q

D

A

M

M

G

G

V

A

Q

R

I

I

E

R

G

-

P

K

D

E

K

G

G

D

N

T

V

W

T

I

I

I

H

D

G

G

V

V

G

G

L

N

R

G

G

G

L

L

K

L

K

A

P

P

E

E

K

A

P

P

M

L

D

D

M

F

G

G

N
|
N

S
x
A

G
x
A

T
|
T

S

G

T

C

R

R

L

L

L

T

A

M

G

G

I

L

L

V

A

G

G

V

Q

Y

D

D

F

F

D

D

C

S

E

T

L

F

V

I

G

G

D

D

A

A

S

S

L

L

S

T

K

K

R
|
R

P

P

M

M

K

G

R

R

V

V

T

L

D

N

P

P

L

L

A

R

M

E

M

M

G

G

A

V

K

Q

I

V

E

K

T

S

A

E

E

D

G

G

G

D

T

R

L

L

P

P

M

V

K

T

I

L

C

R

G

G

H

P

G

-

Q

K

T

T

L

P

K

T

A

P

I

I

D

T

Y

Y

V

R

L

V

P

P

M

M

A

A

S
|
S

A
|
A

Q
|
Q

V

V

K

K

S

S

C

A

V

V

L

L

A

A

G

G

L

L

Y

N

A

T

Q

P

G

G

K

I

T

T

C

T

A

V

E

I

E

E

P

P

A

I

P

M

T

T

R
|
R

D

D

H

H

T

T

E

E

R

K

M

M

L

L

N

Q

G

G

F

F

G

G

Y

A

E

N

V

L

-

T

V

V

S

E

T

T

R

D

L

A

D

D

N

G

S

V

R

R

T

T

R

-

A

I

C

R

L

L

Q

E

G

G

G

R

G

G

S

K

L

L

K

T

A

G

R

Q

H

V

I

I

D

D

V

V

P

P

S

G

D

D

I

P

S

S

A

T

A

A

F

F

F

P

M

L

V

V

A

A

A

L

V

L

S

V

E

P

G

G

S

S

D

D

L

V

V

T

I

I

E

L

H

N

V

V

G

L

M

M

N

N

P

P

T

T

R

R

T

T

G

G

V

L

I

I

D

L

I

T

L

L

K

Q

L

E

M

M

G

G

A

A

N

D

I

I

V

E

L

V

E

I

N

N

A

P

H

R

E

L

V

A

G

G

E

E

P

D

V

V

A

A

D

D

V

L

H

R

I

V

R

R

Y

S

A

S

P

T

L

L

Q

K

G

G

I

V

E

T

I

V

P

P

E

E

S

D

L

R

V

A

P

P

L

S

A

M

I

I

D
|
D

E

E

F

Y

P

P

V

I

L

L

F

A

V

V

A

A

S

F

A

A

T

E

G

G

Q

A

T

T

V

V

L

M

S

N

G

G

A

L

E

E

L

L

R

R

V

V

K
|
K

E
|
E

S

S

D

D

R
|
R

I

L

Q

S

V

A

M

V

A

A

D

N

A

G

L

L

Q

K

A

L

V

N

G

G

I

V

D

D

A

C

V

D

P

E

T

G

P

E

D

T

G

S

M

L

V

V

I

V

N

R

G

G

-

R

-

P

-

D

-

G

-

K

G

G

Q

L

Q

G

S

N

P

A

Q

S

A

G

A

A

E

A

I

V

Q

A

S

T

H

H

H

L

D

D

H
|
H

R
|
R

I

I

S

A

M

M

A

S

M

F

T

L

V

V

A

M

G

G

L

L

N

V

A

S

V

E

S

N

E

P

V

V

V

T

I

V

D

D

C

A

A

T

N

M

V

I

N

A

T
|
T

S

S

F

F

P

P

T

E

F

F

V

M

S

D

L

L

M

M

N

A

E

G

V

L

G

G

M

A

Q

K

I

I

E

E

active site: K23 (= K24), S24 (= S25), D50 (= D51), N93 (= N95), R123 (= R125), D321 (= D324), E349 (= E352), H399 (= H397), R400 (= R398), T426 (= T424)
binding glyphosate: K23 (= K24), A94 (≠ S96), A95 (≠ G97), T96 (= T98), R123 (= R125), D321 (= D324), E349 (= E352), R352 (= R355), R400 (= R398)
binding shikimate-3-phosphate: S24 (= S25), R28 (= R29), S168 (= S171), A169 (= A172), Q170 (= Q173), R195 (= R198), D321 (= D324), K348 (= K351)

2gg6A Cp4 epsp synthase liganded with s3p (see paper)
47% identity, 96% coverage: 14:441/446 of query aligns to 13:443/445 of 2gg6A

query
sites
2gg6A

I

L

H

S

G

G

R

T

I

V

R

R

V

I

P

P

G

G

D

D

K
|
K

S
|
S

I

I

S

S

H

H

R
|
R

S

S

I

F

M

M

L

F

G

G

A

G

I

L

S

A

E

S

G

G

T

E

T

T

T

R

V

I

T

T

G

G

F

L

L

L

E

E

G

G

A

E

D
|
D

A

V

L

I

A

N

T

T

L

G

Q

K

A

A

F

M

K

Q

D

A

M

M

G

G

V

A

Q

R

I

I

E

R

G

-

P

K

D

E

K

G

G

D

N

T

V

W

T

I

I

I

H

D

G

G

V

V

G

G

L

N