SitesBLAST
Comparing WP_028585413.1 NCBI__GCF_000429965.1:WP_028585413.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1mtoA Crystal structure of a phosphofructokinase mutant from bacillus stearothermophilus bound with fructose-6-phosphate (see paper)
39% identity, 94% coverage: 3:344/365 of query aligns to 2:307/319 of 1mtoA
- active site: G11 (= G12), R72 (≠ N74), C73 (≠ M81), D103 (= D117), G104 (= G118), G124 (≠ K138), T125 (= T139), D127 (= D141), D129 (= D143), R171 (= R185)
- binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D141), R162 (= R176), M169 (= M183), R171 (= R185), E222 (= E236), R243 (= R280), H249 (= H286), R252 (= R289)
4pfkA Phosphofructokinase. Structure and control (see paper)
39% identity, 94% coverage: 3:344/365 of query aligns to 2:307/319 of 4pfkA
- active site: G11 (= G12), R72 (≠ N74), C73 (≠ M81), D103 (= D117), G104 (= G118), G124 (≠ K138), T125 (= T139), D127 (= D141), D129 (= D143), R171 (= R185)
- binding adenosine-5'-diphosphate: S9 (≠ G10), Y41 (= Y42), R72 (≠ N74), C73 (≠ M81), F76 (≠ I90), K77 (≠ Q91), G104 (= G118), G108 (≠ I122), R154 (≠ H168), G185 (= G199), R211 (≠ H225), G212 (= G226), K213 (= K227), H215 (≠ Y229)
- binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D141), M169 (= M183), E222 (= E236), H249 (= H286), R252 (= R289)
- binding magnesium ion: G185 (= G199), E187 (≠ D201)
3pfkA Phosphofructokinase. Structure and control (see paper)
39% identity, 94% coverage: 3:344/365 of query aligns to 2:307/319 of 3pfkA
- active site: G11 (= G12), R72 (≠ N74), C73 (≠ M81), D103 (= D117), G104 (= G118), G124 (≠ K138), T125 (= T139), D127 (= D141), D129 (= D143), R171 (= R185)
- binding phosphate ion: R154 (≠ H168), K213 (= K227), H249 (= H286), R252 (= R289)
6pfkA Phosphofructokinase, inhibited t-state (see paper)
39% identity, 94% coverage: 3:344/365 of query aligns to 2:307/319 of 6pfkA
- active site: G11 (= G12), R72 (≠ N74), C73 (≠ M81), D103 (= D117), G104 (= G118), G124 (≠ K138), T125 (= T139), D127 (= D141), D129 (= D143), R171 (= R185)
- binding 2-phosphoglycolic acid: R21 (≠ F22), R25 (≠ K26), G58 (≠ E60), D59 (≠ G61), R154 (≠ H168), R211 (≠ H225), K213 (= K227)
P00512 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 2 papers)
39% identity, 94% coverage: 3:344/365 of query aligns to 2:307/319 of P00512
- RSVVR 21:25 (≠ FAVVK 22:26) binding ADP
- D59 (≠ G61) binding ADP
- R------C 72:73 (≠ NKGNPFAM 74:81) binding ATP
- D103 (= D117) binding Mg(2+)
- TID 125:127 (= TID 139:141) binding in other chain
- R154 (≠ H168) binding in other chain
- R162 (= R176) binding substrate
- MGR 169:171 (= MGR 183:185) binding in other chain
- GAE 185:187 (≠ GAD 199:201) binding in other chain
- R211 (≠ H225) binding in other chain
- KKH 213:215 (≠ KHY 227:229) binding in other chain
- E222 (= E236) binding in other chain
- R243 (= R280) binding substrate
- HVQR 249:252 (≠ HLQR 286:289) binding in other chain
4i4iA Crystal structure of bacillus stearothermophilus phosphofructokinase mutant t156a bound to pep (see paper)
38% identity, 94% coverage: 3:344/365 of query aligns to 2:307/319 of 4i4iA
- active site: G11 (= G12), R72 (≠ N74), C73 (≠ M81), D103 (= D117), G104 (= G118), G124 (≠ K138), T125 (= T139), D127 (= D141), D129 (= D143), R171 (= R185)
- binding phosphoenolpyruvate: R21 (≠ F22), R25 (≠ K26), G58 (≠ E60), R154 (≠ H168), R211 (≠ H225), K213 (= K227), H215 (≠ Y229)
1pfkA Crystal structure of the complex of phosphofructokinase from escherichia coli with its reaction products (see paper)
37% identity, 94% coverage: 1:342/365 of query aligns to 1:306/320 of 1pfkA
- active site: G12 (= G12), R73 (≠ N74), F74 (vs. gap), D104 (= D117), G105 (= G118), G125 (≠ K138), T126 (= T139), D128 (= D141), D130 (= D143), R172 (= R185)
- binding adenosine-5'-diphosphate: G11 (= G11), R22 (≠ F22), R26 (≠ K26), Y56 (≠ E57), S59 (≠ E60), D60 (≠ G61), R73 (≠ N74), F74 (vs. gap), F77 (vs. gap), R78 (vs. gap), G103 (= G116), D104 (= D117), G105 (= G118), S106 (= S119), M108 (≠ E121), G109 (≠ I122), R155 (≠ H168), G213 (= G226), K214 (= K227), H216 (≠ Y229)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: G12 (= G12), R73 (≠ N74), T126 (= T139), D128 (= D141), M170 (= M183), E223 (= E236), H250 (= H286), R253 (= R289)
- binding magnesium ion: G186 (= G199), E188 (≠ D201)
P0A796 ATP-dependent 6-phosphofructokinase isozyme 1; ATP-PFK 1; Phosphofructokinase 1; 6-phosphofructokinase isozyme I; Phosphohexokinase 1; Sedoheptulose-7-phosphate kinase; EC 2.7.1.11 from Escherichia coli (strain K12) (see 2 papers)
37% identity, 94% coverage: 1:342/365 of query aligns to 1:306/320 of P0A796
- G12 (= G12) binding ATP
- RGVVR 22:26 (≠ FAVVK 22:26) binding ADP
- RYSVSD 55:60 (≠ PESVEG 56:61) binding ADP
- RF 73:74 (≠ N- 74) binding ATP
- GDGS 103:106 (= GDGS 116:119) binding ATP
- D104 (= D117) binding Mg(2+)
- TID 126:128 (= TID 139:141) binding in other chain
- D128 (= D141) active site, Proton acceptor; mutation to S: 18000-fold reduction of catalytic rate.
- R155 (≠ H168) binding in other chain
- R163 (= R176) binding substrate
- R172 (= R185) mutation to S: 3.4-fold reduction in turnover numbers.
- GCE 186:188 (≠ GAD 199:201) binding in other chain
- KKH 214:216 (≠ KHY 227:229) binding in other chain
- E223 (= E236) binding in other chain
- R244 (= R280) binding substrate
- HIQR 250:253 (≠ HLQR 286:289) binding in other chain
5xzaA Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adp (see paper)
38% identity, 85% coverage: 3:311/365 of query aligns to 2:276/322 of 5xzaA
Q2FXM8 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
38% identity, 85% coverage: 3:311/365 of query aligns to 2:276/322 of Q2FXM8
- R------------C 72:73 (≠ NKGNPFAMPVENLA 74:87) binding ATP
- GDGS 102:105 (= GDGS 116:119) binding ATP
- TID 127:129 (= TID 139:141) binding in other chain
- G150 (≠ D162) mutation to D: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of dimer conversion; in association with A-151.
- L151 (≠ A163) mutation to A: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of tetramer-dimer conversion; in association with D-150.
- R164 (= R176) mutation to A: Complete loss of fructose 6-phosphate binding.
- MGR 171:173 (= MGR 183:185) binding in other chain
- E224 (= E236) binding in other chain
- R245 (= R280) mutation to A: Complete loss of fructose 6-phosphate binding.
- HVQR 251:254 (≠ HLQR 286:289) binding in other chain
5xz7A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
38% identity, 85% coverage: 3:311/365 of query aligns to 2:276/322 of 5xz7A
5xz9A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
38% identity, 85% coverage: 3:311/365 of query aligns to 2:276/321 of 5xz9A
- binding adenosine-5'-triphosphate: S9 (≠ G10), Y41 (= Y42), C73 (≠ A87), F76 (≠ I90), K77 (≠ Q91), G102 (= G116), D103 (= D117), G104 (= G118), S105 (= S119), R107 (≠ E121), G108 (≠ I122)
5xz6A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
38% identity, 85% coverage: 3:311/365 of query aligns to 2:276/318 of 5xz6A
3o8lA Structure of phosphofructokinase from rabbit skeletal muscle (see paper)
35% identity, 84% coverage: 5:309/365 of query aligns to 10:313/748 of 3o8lA
- active site: G17 (= G12), R80 (≠ N74), D111 (= D117), S156 (≠ T139), D158 (= D141), D160 (= D143), R202 (= R185)
- binding adenosine-5'-diphosphate: D165 (= D148), M166 (≠ R149), F300 (= F296)
- binding adenosine-5'-triphosphate: G16 (= G11), Y47 (= Y42), R80 (≠ N74), C81 (≠ A80), R85 (≠ E84), G110 (= G116), G112 (= G118), S113 (= S119), T115 (≠ E121), G116 (≠ I122), W219 (≠ V202), H234 (≠ A214), R238 (≠ K218)
Sites not aligning to the query:
P00511 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
35% identity, 84% coverage: 5:309/365 of query aligns to 18:321/780 of P00511
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylthreonine
- 775 modified: Phosphoserine; by PKA
P08237 ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; PFK-M; 6-phosphofructokinase type A; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphohexokinase; EC 2.7.1.11 from Homo sapiens (Human) (see 6 papers)
34% identity, 84% coverage: 5:309/365 of query aligns to 18:321/780 of P08237
- R39 (≠ K26) to P: in GSD7; Italian; dbSNP:rs121918193
- G57 (= G44) to V: in GSD7; Italian
- R100 (≠ A87) to Q: in dbSNP:rs2228500
- S180 (≠ T155) to C: in GSD7; Italian
- G209 (= G184) to D: in GSD7; loss of activity shown by complementation assays in yeast; dbSNP:rs767265360
- D309 (= D297) to G: in GSD7; Spanish; complete loss of enzyme activity; dbSNP:rs1169383137
Sites not aligning to the query:
- 543 D → A: in GSD7; Italian; dbSNP:rs121918194
- 557 modified: N6-(2-hydroxyisobutyryl)lysine
- 591 D → A: in GSD7; Italian
- 686 W → C: in GSD7; Japanese; dbSNP:rs121918196
- 696 R → H: in dbSNP:rs41291971
P47859 ATP-dependent 6-phosphofructokinase, platelet type; ATP-PFK; PFK-P; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphohexokinase; EC 2.7.1.11 from Oryctolagus cuniculus (Rabbit) (see paper)
34% identity, 93% coverage: 9:349/365 of query aligns to 31:373/791 of P47859
Sites not aligning to the query:
- 12 modified: Phosphoserine; by PKA
4rh3A Amppcp-bound structure of human platelet phosphofructokinase in an r- state, crystal form ii (see paper)
33% identity, 94% coverage: 7:349/365 of query aligns to 5:349/731 of 4rh3A
- active site: G10 (= G12), R73 (vs. gap), C74 (vs. gap), D104 (vs. gap), G148 (≠ K138), S149 (≠ T139), D151 (= D141), D153 (= D143), R195 (= R185)
- binding phosphomethylphosphonic acid adenylate ester: G9 (= G11), R73 (vs. gap), C74 (vs. gap), F77 (vs. gap), R78 (vs. gap), G103 (vs. gap), D104 (vs. gap), G105 (= G88), S106 (≠ E89), G109 (≠ V92)
- binding phosphate ion: K240 (= K227)
Sites not aligning to the query:
4xyjF Crystal structure of human phosphofructokinase-1 in complex with atp and mg, northeast structural genomics consortium target hr9275 (see paper)
33% identity, 94% coverage: 7:349/365 of query aligns to 15:359/761 of 4xyjF
- active site: G20 (= G12), R83 (vs. gap), C84 (vs. gap), D114 (vs. gap), G158 (≠ K138), S159 (≠ T139), D161 (= D141), D163 (= D143), R205 (= R185)
- binding adenosine-5'-triphosphate: G20 (= G12), Y50 (= Y42), R83 (vs. gap), C84 (vs. gap), F87 (vs. gap), R88 (vs. gap), G113 (vs. gap), D114 (vs. gap), G115 (= G88), S116 (≠ E89), G119 (≠ V92), S159 (≠ T139)
- binding magnesium ion: G19 (= G11), G20 (= G12), G112 (vs. gap), D114 (vs. gap), G158 (≠ K138), D163 (= D143), R205 (= R185)
- binding phosphate ion: R30 (≠ F22), R34 (≠ K26), S69 (≠ E60), S70 (≠ G61), G219 (= G199), K250 (= K227)
Sites not aligning to the query:
4xykA Crystal structure of human phosphofructokinase-1 in complex with adp, northeast structural genomics consortium target hr9275 (see paper)
33% identity, 94% coverage: 7:349/365 of query aligns to 13:357/737 of 4xykA
- active site: G18 (= G12), R81 (vs. gap), C82 (vs. gap), D112 (vs. gap), G156 (≠ K138), S157 (≠ T139), D159 (= D141), D161 (= D143), R203 (= R185)
- binding adenosine-5'-diphosphate: Y48 (= Y42), R81 (vs. gap), C82 (vs. gap), R86 (vs. gap), G111 (vs. gap), D112 (vs. gap), G113 (= G88), S114 (≠ E89)
- binding phosphate ion: R28 (≠ F22), R32 (≠ K26), S67 (≠ E60), K248 (= K227)
Sites not aligning to the query:
Query Sequence
>WP_028585413.1 NCBI__GCF_000429965.1:WP_028585413.1
MREKILISTGGGDAPGLNAVIFAVVKAATRKGWEVYGSRSGYSGLLDRDDLIRLTPESVE
GILPIGGTILGSTNKGNPFAMPVENLAGEIQVVDVSEKIMASFKRMGFLCHIAVGGDGSL
EIAHRFAQLGMPVIGVPKTIDNDLEVTDRTFGFDTAVSTATDAIDKLHSTAKSHDRVMVV
EVMGRDSGWIALYSGVSGGADVILVPEIPFSLDAVCEKIADNELHGKHYSIVVVAEGATA
ENGEVITKGKGELGREEAVLGGIGEWVANEIRERLGKDTRSLVLGHLQRGGSPTTFDRLL
ALRFGAAAVRMAEAEIFDHMVAWTPPHMISVPIADAICRRKKIDLSSDKVLTAREIGICL
GDTQE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory