SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028949747.1 NCBI__GCF_000619805.1:WP_028949747.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q42601 Carbamoyl phosphate synthase arginine-specific large chain, chloroplastic; CPS; CPSase; Ammonium-dependent carbamoyl phosphate synthetase; Arginine-specific carbamoyl phosphate synthetase, ammonia chain; Glutamine-dependent carbamoyl phosphate synthetase; Protein VENOSA 3; EC 6.3.4.16; EC 6.3.5.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
65% identity, 96% coverage: 3:540/559 of query aligns to 94:639/1187 of Q42601

query
sites
Q42601

K

K

R

R

T

T

D

D

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E

K

T

K

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M

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A

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P

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G

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A

A

C

E

E

F

F

D

D

Y

Y

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Q

G

A

A

C

K

K

A

A

L

L

K

R

E

E

G

G

Y

Y

R

E

V

V

I

I

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L

V

I

N

N

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S

N

N

P

P

A

A

T

T

I

I

M

M

T

T

D

D

P
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P

N

E

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T

A

A

D

N

R

R

T

T

Y

Y

I

I

E

A

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L

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E

K

Q

I

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K

K

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E

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R

P

P

D

D

A

A

I

L

L

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P

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T

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M

G

G

Q

Q

T

T

A

A

L

L

N

N

L

L

A

A

I

V

D

A

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L

Y

A

E

E

K

S

G

G

I

A

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D

E

K

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Y

Y

N

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V

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E

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A

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A

A

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K

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Y

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N

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D

N

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C

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N

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D

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C

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N

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P

K

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G

G

E

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M

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L

A

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A

A

K

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A

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V

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G

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Y

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N

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-

D

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N

D

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A

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A

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M

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K

E

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D

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Y

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G

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A

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D

P149 (= P58) mutation to L: In ven3-2; reduced plant size and reticulate leaf phenotype.
G587 (= G492) mutation to E: In ven3-3; reticulate leaf phenotype.

Sites not aligning to the query:

844 A→T: In ven3-4; reduced plant size and reticulate leaf phenotype.
1014 P→L: In ven3-1; reticulate leaf phenotype.

1bxrA Structure of carbamoyl phosphate synthetase complexed with the atp analog amppnp (see paper)
62% identity, 97% coverage: 1:540/559 of query aligns to 1:548/1073 of 1bxrA

query
sites
1bxrA

M

M

P

P

K

K

R

R

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T

D

D

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K

T

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A

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P

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G

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Q

A

A

A

C

E

E

F

F

D

D

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Y

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G

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A

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Q

G

A

A

C

K

K

A

A

L

L

K

R

E

E

G

G

Y

Y

R

R

V

V

I

I

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L

V

V

N

N

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S

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N

P

P

A

A

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T

I

I

M

M

T

T

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D

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P

N

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I

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Y

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I

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K

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P

D

D

A

A

I

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L

L

P

P

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G

Q

Q

T

T

A

A

L

L

N

N

L

C

A

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A

A

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D

D

R
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R

D

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I

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R

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F

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L
x
M

G
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G

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G

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Y

N

N

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R

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K

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x
L

I
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I

G

G

W

W

K

K

E
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E

F

Y

E

E

M

M

E

E

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M

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R

R

D

D

K

K

N

N

D

D

N

N

C

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C

S

S

I

I

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E

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H

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T

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A

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Q

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A

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A

A

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V

M

L

R

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E

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G

G

S

S

N
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N

V

V

Q
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Q

F

F

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N

N

P

P

K

K

T

N

G

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I

I

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E

M

M

N
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N

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R

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R

S
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S

S

S

A

A

L

L

A

A

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K

K

A

A

T

T

G

G

F

F

P

P

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I

A

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A

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L

A

A

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V

G

G

Y

Y

T

T

L

L

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E

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L

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M

N

N

D
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D

I

I

T

T

-

G

K

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P

A

A

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S

F

F

E

E

P

P

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I

I

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D

Y

Y

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K

K

I

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Q

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K

A

A

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Y

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G

F

F

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D

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Y

Y

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I

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K

A

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G

Y

L

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N

F

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N

I

I

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K

L

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A

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G

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A

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K
x
G

T

V

T

R

E

E

E

A

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E

V

I

R

R

K

K

R

L

K

R

-

D

-

Q

-

Y

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D

I

L

I

H

V

P

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V

Y

Y

K

K

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R

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V

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D

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T

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C

A

A

E

E

F

F

E

A

A

T

F

D

T

T

P

A

Y

Y

Y

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Y

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S

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Y

Y

E

E

active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (≠ H202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (= D338), G507 (≠ K503)
binding phosphoaminophosphonic acid-adenylate ester: R129 (= R129), I167 (= I167), R169 (= R169), M174 (≠ L174), G175 (= G175), G176 (= G176), L210 (≠ V210), I211 (= I211), E215 (= E215), M240 (= M240), G241 (= G241), H243 (= H243), T244 (= T244), Q285 (= Q285), E299 (= E299), R306 (= R306), T376 (= T375)
binding manganese (ii) ion: E299 (= E299), N301 (= N301)

Sites not aligning to the query:

active site: 634, 715, 721, 722, 745, 761, 769, 829, 841, 843, 848, 901
binding phosphoaminophosphonic acid-adenylate ester: 675, 713, 715, 720, 721, 722, 725, 753, 755, 756, 761, 785, 786, 787, 788, 829, 841, 843, 848
binding manganese (ii) ion: 829, 841, 841, 843
binding L-ornithine: 783, 791, 892, 907, 1041, 1042

P00968 Carbamoyl phosphate synthase large chain; Carbamoyl phosphate synthetase ammonia chain; EC 6.3.4.16; EC 6.3.5.5 from Escherichia coli (strain K12) (see 6 papers)
62% identity, 97% coverage: 1:540/559 of query aligns to 1:548/1073 of P00968

query
sites
P00968

M
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M

P

P

K

K

R

R

T

T

D

D

I

I

E

K

T

S

I

I

L

L

L

I

I

L

G

G

S

A

G

G

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P

I

I

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I

I

G

G

Q

Q

A

A

A

C

E

E

F

F

D

D

Y

Y

S

S

G

G

T

A

Q

Q

G

A

A

C

K

K

A

A

L

L

K

R

E

E

G

G

Y

Y

R

R

V

V

I

I

L

L

V

V

N

N

S

S

N

N

P

P

A

A

T

T

I

I

M

M

T

T

D

D

P

P

N

E

I

M

A

A

D

D

R

A

T

T

Y

Y

I

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E

E

P

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L

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I

H

T

W

P

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V

L

V

E

R

K

K

I

I

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E

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K

E

E

R

R

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P

D

D

A

A

I

V

L

L

P

P

T

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L

M

G

G

Q

Q

T

T

A

A

L

L

N

N

L

C

A

A

I

L

D

E

L

L

Y

E

E

R

K

Q

G

G

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L

D

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Y

F

N

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M

I

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G

G

A

A

K

T

Y

A

E

D

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A

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I

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D

K

K

A

A

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E

D

D

R
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R

D

R

L

R

F

F

K

D

K

V

A

A

M

M

E

K

N

K

I

I

G

G

L

L

S

E

M

T

P

A

K

R

S

S

A

G

V

I

V

A

K

H

S

T

V

M

A

E

E

E

A

A

E

L

E

A

V

V

I

A

D

A

W

D

I

V

G

G

F

F

P

P

V

C

I

I

R
|
R

P

P

S

S

F

F

T

T

L

M

G
|
G

T

S

G

G

S

G

I

I

A

A

Y

Y

N

N

R

R

E

E

F

F

Y

E

P

E

K

I

V

C

K

A

A

R

G

G

L

L

D

D

A

L

S

S

P

P

I

T

H

K

E

E

V

L

L

L

L

I

E

D

E
|
E

S

S

V
x
L

I

I

G

G

W

W

K

K

E
|
E

F

Y

E

E

M

M

E

E

V

V

M

V

R

R

D

D

K

K

N

N

D

D

N

N

C

C

V

I

I

I

I

V

C

C

S

S

I

I

E

E

N

N

L

F

D

D

P

A

M

M

G
|
G

V
x
I

H
|
H

T

T

G

G

D

D

S

S

I

I

T

T

I

V

A

A

P

P

A

A

M

Q

T

T

L

L

S

T

D

D

R

K

E

E

Y

Y

Q

Q

I

I

L

M

R

R

N

N

Y

A

S

S

I

M

A

A

V

V

M

L

R

R

E

E

I

I

G

G

V

V

E

E

T

T

G

G

S

S

N

N

V

V

Q
|
Q

F

F

S

A

Q

V

N

N

P

P

K

K

T

N

G

G

E

R

F

L

Y

I

V

V

I

I

E
|
E

M

M

N
|
N

P

P

R

R

V

V

S

S

R

R

S

S

A

A

L

L

A

A

S

S

K

K

A

A

T

T

G

G

F

F

P

P

I

I

A

A

K

K

I

V

A

A

K

K

L

L

A

A

V

V

G

G

Y

Y

T

T

L

L

D

D

E

E

L

L

P

M

N

N

D

D

I

I

T

T

-

G

K

G

K

R

T

T

P

P

A

A

S

S

F

F

E

E

P

P

T

S

I

I

D

D

Y

Y

V

V

V

T

K

K

I

I

P

P

R

R

F

F

D

N

F

F

A

E

K

K

F

F

P

A

E

G

A

A

D

N

P

D

T

R

L

L

T

T

M

Q

M

M

K

K

S

S

V

V

G

G

E

E

V

V

M

M

A

A

I

I

G

G

R

R

T

T

F

Q

K

Q

E

E

S

S

L

L

Q

Q

K

K

A

A

L

L

R

R

S

G

L

L

E

E

L

V

G

G

R

A

Y

T

G

G

F

F

-

D

-

P

Y

K

M

V

G

S

L

L

E

D

N

D

I

P

D

E

E

A

N

L

T

T

-

K

L

I

K

R

E

R

K

E

I

L

I

K

K

D

P

A

N

G

P

A

D

D

R

R

L

I

W

W

Y

Y

I

I

A

A

E

D

A

A

F

F

R

R

K

A

G

G

Y

L

T

S

V

V

E

D

E

G

I

V

N

F

K

N

L

L

S

T

H

N

I

I

D

D

K

R

W

W

F

F

L

L

N

V

Q

Q

I

I

K

E

E

E

I

L

I

V

D

R

Y

L

E

E

L

E

Y

K

L

V

S

A

T

E

K

V

T

G

I

I

D

T

T

G

I

L

T

N

D

A

Q

D

E

F

L

L

E

R

K

Q

A

L

K

K

Q

R

W

K

G

G

F

F

S

A

D

D

R

A

E

R

L

L

A

A

R

K

L

L

L

A

K

G

T

V

T

R

E

E

E

A

E

E

V

I

R

R

K

K

R

L

K

R

-

D

-

Q

-

Y

-

D

I

L

I

H

V

P

S

V

Y

Y

K

K

V

R

V

V

D

D

T

T

C

C

A

A

E

E

F

F

E

A

A

T

F

D

T

T

P

A

Y

Y

Y

M

Y

Y

S

S

S

T

Y

Y

E

E

M1 (= M1) modified: Initiator methionine, Removed
R129 (= R129) binding ATP
R169 (= R169) binding ATP
G175 (= G175) binding ATP
G176 (= G176) binding ATP
E208 (= E208) binding ATP
L210 (≠ V210) binding ATP
E215 (= E215) binding ATP
G241 (= G241) binding ATP
I242 (≠ V242) binding ATP
H243 (= H243) binding ATP
Q285 (= Q285) binding ATP; binding Mn(2+)
E299 (= E299) binding ATP; binding Mn(2+); binding Mn(2+)
N301 (= N301) binding Mn(2+)

Sites not aligning to the query:

715 binding ATP
754 binding ATP
756 binding ATP
761 binding ATP
786 binding ATP
787 binding ATP
788 binding ATP
789 binding ATP
829 binding ATP; binding Mn(2+)
841 binding ATP; binding Mn(2+); binding Mn(2+)
843 binding Mn(2+)

1t36A Crystal structure of e. Coli carbamoyl phosphate synthetase small subunit mutant c248d complexed with uridine 5'-monophosphate (see paper)
62% identity, 97% coverage: 1:540/559 of query aligns to 1:548/1058 of 1t36A

query
sites
1t36A

M

M

P

P

K

K

R

R

T

T

D

D

I

I

E

K

T

S

I

I

L

L

L

I

I

L

G

G

S

A

G

G

P

P

I

I

V

V

I

I

G

G

Q

Q

A

A

A

C

E

E

F

F

D

D

Y

Y

S

S

G

G

T

A

Q

Q

G

A

A

C

K

K

A

A

L

L

K

R

E

E

G

G

Y

Y

R

R

V

V

I

I

L

L

V

V

N

N

S

S

N

N

P

P

A

A

T

T

I

I

M

M

T

T

D

D

P

P

N

E

I

M

A

A

D

D

R

A

T

T

Y

Y

I

I

E

E

P

P

L

I

I

H

T

W

P

E

V

V

L

V

E

R

K

K

I

I

E

E

K

K

E

E

R

R

P

P

D

D

A

A

I

V

L

L

P

P

T

T

L

M

G

G

Q

Q

T

T

A

A

L

L

N

N

L

C

A

A

I

L

D

E

L

L

Y

E

E

R

K

Q

G

G

I

V

L

L

D

E

K

E

Y

F

N

G

V

V

E

T

L

M

I

I

G

G

A

A

K

T

Y

A

E

D

V

A

I

I

K

D

K

K

A

A

E

E

D

D

R
|
R

D

R

L

R

F

F

K

D

K

V

A

A

M

M

E

K

N

K

I

I

G

G

L

L

S

E

M

T

P

A

K

R

S

S

A

G

V

I

V

A

K

H

S

T

V

M

A

E

E

E

A

A

E

L

E

A

V

V

I

A

D

A

W

D

I

V

G

G

F

F

P

P

V

C

I
|
I

I

I

R
|
R

P

P

S

S

F

F

T

T

L
x
M

G
|
G

T

S

G

G

S

G

I

I

A

A

Y

Y

N

N

R

R

E

E

F

F

Y

E

P

E

K

I

V

C

K

A

A

R

G

G

L

L

D

D

A

L

S

S

P

P

I

T

H
x
K

E

E

V

L

L

L

L

I

E

D

E
|
E

S

S

V
x
L

I
|
I

G

G

W

W

K

K

E
|
E

F

Y

E

E

M

M

E

E

V

V

M

V

R

R

D

D

K

K

N

N

D

D

N

N

C

C

V

I

I

I

I

V

C

C

S

S

I

I

E

E

N

N

L

F

D

D

P

A

M
|
M

G
|
G

V
x
I

H
|
H

T

T

G

G

D

D

S

S

I

I

T

T

I

V

A

A

P

P

A

A

M

Q

T

T

L

L

S

T

D

D

R

K

E

E

Y

Y

Q

Q

I

I

L

M

R

R

N

N

Y

A

S

S

I

M

A

A

V

V

M

L

R

R

E

E

I

I

G

G

V

V

E

E

T

T

G

G

S

S

N
|
N

V

V

Q
|
Q

F

F

S

A

Q

V

N

N

P

P

K

K

T

N

G

G

E

R

F

L

Y

I

V

V

I
|
I

E
|
E

M

M

N
|
N

P

P

R
|
R

V

V

S

S

R
|
R

S
|
S

S

S

A

A

L

L

A

A

S

S

K

K

A

A

T

T

G

G

F

F

P

P

I

I

A

A

K

K

I

V

A

A

K

K

L

L

A

A

V

V

G

G

Y

Y

T

T

L

L

D

D

E

E

L

L

P

M

N

N

D
|
D

I

I

T

T

-

G

K

G

K

R

T

T

P

P

A

A

S

S

F

F

E

E

P

P

T

S

I

I

D

D

Y

Y

V

V

V

T

K

K

I

I

P

P

R

R

F

F

D

N

F

F

A

E

K

K

F

F

P

A

E

G

A

A

D

N

P

D

T

R

L

L

T

T

T
|
T

M

Q

M

M

K

K

S

S

V

V

G

G

E

E

V

V

M

M

A

A

I

I

G

G

R

R

T

T

F

Q

K

Q

E

E

S

S

L

L

Q

Q

K

K

A

A

L

L

R

R

S

G

L

L

E

E

L

V

G

G

R

A

Y

T

G

G

F

F

-

D

-

P

Y

K

M

V

G

S

L

L

E

D

N

D

I

P

D

E

E

A

N

L

T

T

-

K

L

I

K

R

E

R

K

E

I

L

I

K

K

D

P

A

N

G

P

A

D

D

R

R

L

I

W

W

Y

Y

I

I

A

A

E

D

A

A

F

F

R

R

K

A

G

G

Y

L

T

S

V

V

E

D

E

G

I

V

N

F

K

N

L

L

S

T

H

N

I

I

D

D

K

R

W

W

F

F

L

L

N

V

Q

Q

I

I

K

E

E

E

I

L

I

V

D

R

Y

L

E

E

L

E

Y

K

L

V

S

A

T

E

K

V

T

G

I

I

D

T

T

G

I

L

T

N

D

A

Q

D

E

F

L

L

E

R

K

Q

A

L

K

K

Q

R

W

K

G

G

F

F

S

A

D

D

R

A

E

R

L

L

A

A

R

K

L

L

L

A

K
x
G

T

V

T

R

E

E

E

A

E

E

V

I

R

R

K

K

R

L

K

R

-

D

-

Q

-

Y

-

D

I

L

I

H

V

P

S

V

Y

Y

K

K

V

R

V

V

D

D

T

T

C

C

A

A

E

E

F

F

E

A

A

T

F

D

T

T

P

A

Y

Y

Y

M

Y

Y

S

S

S

T

Y

Y

E

E

active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (≠ H202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (= D338), G507 (≠ K503)
binding adenosine-5'-diphosphate: R129 (= R129), I167 (= I167), R169 (= R169), M174 (≠ L174), G175 (= G175), G176 (= G176), E208 (= E208), L210 (≠ V210), I211 (= I211), E215 (= E215), M240 (= M240), G241 (= G241), I242 (≠ V242), H243 (= H243), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (= T375)
binding manganese (ii) ion: Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301)
binding phosphate ion: M174 (≠ L174), G175 (= G175), H243 (= H243), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)

Sites not aligning to the query:

active site: 634, 715, 746, 754, 814, 826, 828, 833, 886
binding adenosine-5'-diphosphate: 715, 718, 740, 741, 746, 770, 771, 772, 773, 826, 894
binding manganese (ii) ion: 814, 826
binding L-ornithine: 768, 776, 877, 892, 1026, 1027
binding uridine-5'-monophosphate: 939, 959, 961, 962, 978, 1000, 1001, 1002, 1011, 1014

1c3oA Crystal structure of the carbamoyl phosphate synthetase: small subunit mutant c269s with bound glutamine (see paper)
62% identity, 97% coverage: 1:540/559 of query aligns to 1:548/1058 of 1c3oA

query
sites
1c3oA

M

M

P

P

K

K

R

R

T

T

D

D

I

I

E

K

T

S

I

I

L

L

L

I

I

L

G

G

S

A

G

G

P

P

I

I

V

V

I

I

G

G

Q

Q

A

A

A

C

E

E

F

F

D

D

Y

Y

S

S

G

G

T

A

Q

Q

G

A

A

C

K

K

A

A

L

L

K

R

E

E

G

G

Y

Y

R

R

V

V

I

I

L

L

V

V

N

N

S

S

N

N

P

P

A

A

T

T

I

I

M

M

T

T

D

D

P

P

N

E

I

M

A

A

D

D

R

A

T

T

Y

Y

I

I

E

E

P

P

L

I

I

H

T

W

P

E

V

V

L

V

E

R

K

K

I

I

E

E

K

K

E

E

R

R

P

P

D

D

A

A

I

V

L

L

P

P

T

T

L

M

G

G

Q

Q

T

T

A

A

L

L

N

N

L

C

A

A

I

L

D

E

L

L

Y

E

E

R

K

Q

G

G

I

V

L

L

D

E

K

E

Y

F

N

G

V

V

E

T

L

M

I

I

G

G

A

A

K

T

Y

A

E

D

V

A

I

I

K

D

K

K

A

A

E

E

D

D

R
|
R

D

R

L

R

F

F

K

D

K

V

A

A

M

M

E

K

N

K

I

I

G

G

L

L

S

E

M

T

P

A

K

R

S

S

A

G

V

I

V

A

K

H

S

T

V

M

A

E

E

E

A

A

E

L

E

A

V

V

I

A

D

A

W

D

I

V

G

G

F

F

P

P

V

C

I
|
I

I

I

R
|
R

P

P

S

S

F

F

T

T

L
x
M

G
|
G

T

S

G

G

S

G

I

I

A

A

Y

Y

N

N

R

R

E

E

F

F

Y

E

P

E

K

I

V

C

K

A

A

R

G

G

L

L

D

D

A

L

S

S

P

P

I

T

H
x
K

E

E

V

L

L

L

L

I

E

D

E

E

S

S

V
x
L

I
|
I

G

G

W

W

K

K

E
|
E

F

Y

E

E

M

M

E

E

V

V

M

V

R

R

D

D

K

K

N

N

D

D

N

N

C

C

V

I

I

I

I

V

C

C

S

S

I

I

E

E

N

N

L

F

D

D

P

A

M
|
M

G
|
G

V

I

H
|
H

T
|
T

G

G

D

D

S

S

I

I

T

T

I

V

A

A

P

P

A

A

M

Q

T

T

L

L

S

T

D

D

R

K

E

E

Y

Y

Q

Q

I

I

L

M

R

R

N

N

Y

A

S

S

I

M

A

A

V

V

M

L

R

R

E

E

I

I

G

G

V

V

E

E

T

T

G

G

S

S

N
|
N

V

V

Q
|
Q

F

F

S

A

Q

V

N

N

P

P

K

K

T

N

G

G

E

R

F

L

Y

I

V

V

I
|
I

E
|
E

M

M

N
|
N

P

P

R
|
R

V

V

S

S

R
|
R

S
|
S

S

S

A

A

L

L

A

A

S

S

K

K

A

A

T

T

G

G

F

F

P

P

I

I

A

A

K

K

I

V

A

A

K

K

L

L

A

A

V

V

G

G

Y

Y

T

T

L

L

D

D

E

E

L

L

P

M

N

N

D
|
D

I

I

T

T

-

G

K

G

K

R

T

T

P

P

A

A

S

S

F

F

E

E

P

P

T

S

I

I

D

D

Y

Y

V

V

V

T

K

K

I

I

P

P

R

R

F

F

D

N

F

F

A

E

K

K

F

F

P

A

E

G

A

A

D

N

P

D

T

R

L

L

T

T

T
|
T

M

Q

M

M

K

K

S

S

V

V

G

G

E

E

V

V

M

M

A

A

I

I

G

G

R

R

T

T

F

Q

K

Q

E

E

S

S

L

L

Q

Q

K

K

A

A

L

L

R

R

S

G

L

L

E

E

L

V

G

G

R

A

Y

T

G

G

F

F

-

D

-

P

Y

K

M

V

G

S

L

L

E

D

N

D

I

P

D

E

E

A

N

L

T

T

-

K

L

I

K

R

E

R

K

E

I

L

I

K

K

D

P

A

N

G

P

A

D

D

R

R

L

I

W

W

Y

Y

I

I

A

A

E

D

A

A

F

F

R

R

K

A

G

G

Y

L

T

S

V

V

E

D

E

G

I

V

N

F

K

N

L

L

S

T

H

N

I

I

D

D

K

R

W

W

F

F

L

L

N

V

Q

Q

I

I

K

E

E

E

I

L

I

V

D

R

Y

L

E

E

L

E

Y

K

L

V

S

A

T

E

K

V

T

G

I

I

D

T

T

G

I

L

T

N

D

A

Q

D

E

F

L

L

E

R

K

Q

A

L

K

K

Q

R

W

K

G

G

F

F

S

A

D

D

R

A

E

R

L

L

A

A

R

K

L

L

L

A

K
x
G

T

V

T

R

E

E

E

A

E

E

V

I

R

R

K

K

R

L

K

R

-

D

-

Q

-

Y

-

D

I

L

I

H

V

P

S

V

Y

Y

K

K

V
x
R

V

V

D

D

T

T

C

C

A

A

E

E

F

F

E
x
A

A
x
T

F

D

T

T

P

A

Y

Y

Y

M

Y

Y

S

S

S

T

Y

Y

E

E

active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (≠ H202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (= D338), G507 (≠ K503)
binding adenosine-5'-diphosphate: R129 (= R129), I167 (= I167), R169 (= R169), M174 (≠ L174), G176 (= G176), L210 (≠ V210), I211 (= I211), E215 (= E215), M240 (= M240), G241 (= G241), H243 (= H243), T244 (= T244), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (= T375)
binding glutamine: R528 (≠ V520), A537 (≠ E529), T538 (≠ A530)
binding manganese (ii) ion: Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301)
binding phosphate ion: M174 (≠ L174), G175 (= G175), H243 (= H243), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)

Sites not aligning to the query:

active site: 634, 715, 746, 754, 814, 826, 828, 833, 886
binding adenosine-5'-diphosphate: 715, 718, 740, 741, 746, 770, 771, 772, 773, 774, 826
binding glutamine: 554
binding manganese (ii) ion: 814, 826
binding L-ornithine: 768, 776, 877, 892, 1026, 1027

1a9xA Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis (see paper)
62% identity, 97% coverage: 1:540/559 of query aligns to 1:548/1058 of 1a9xA

query
sites
1a9xA

M

M

P

P

K

K

R

R

T

T

D

D

I

I

E

K

T

S

I

I

L

L

L

I

I

L

G

G

S

A

G

G

P

P

I

I

V

V

I

I

G

G

Q

Q

A

A

A

C

E

E

F

F

D

D

Y

Y

S

S

G

G

T

A

Q

Q

G

A

A

C

K

K

A

A

L

L

K

R

E

E

G

G

Y

Y

R

R

V

V

I

I

L

N

V

V

N

N

S

S

N

N

P

P

A

A

T

T

I

I

M

M

T

T

D

D

P

P

N

E

I

M

A

A

D

D

R

A

T

T

Y

Y

I

I

E

E

P

P

L

I

I

H

T

W

P

E

V

V

L

V

E

R

K

K

I

I

E

E

K

K

E

E

R

R

P

P

D

D

A

A

I

V

L

L

P

P

T

T

L

M

G

G

Q

Q

T

T

A

A

L

L

N

N

L

C

A

A

I

L

D

E

L

L

Y

E

E

R

K

Q

G

G

I

V

L

L

D

E

K

E

Y

F

N

G

V

V

E

T

L

M

I

I

G

G

A

A

K

T

Y

A

E

D

V

A

I

I

K

D

K

K

A

A

E

E

D

D

R
|
R

D

R

L

R

F

F

K

D

K

V

A

A

M

M

E

K

N

K

I

I

G

G

L

L

S

E

M

T

P

A

K

R

S

S

A

G

V

I

V

A

K

H

S

T

V

M

A

E

E

E

A

A

E

L

E

A

V

V

I

A

D

A

W

D

I

V

G

G

F

F

P

P

V

C

I
|
I

I

I

R
|
R

P

P

S

S

F

F

T

T

L
x
M

G
|
G

T

S

G

G

S

G

I

I

A

A

Y

Y

N

N

R

R

E

E

F

F

Y

E

P

E

K

I

V

C

K

A

A

R

G

G

L

L

D

D

A

L

S

S

P

P

I

T

H
x
K

E

E

V

L

L

L

L

I

E

D

E

E

S

S

V
x
L

I

I

G

G

W

W

K

K

E
|
E

F

Y

E

E

M

M

E

E

V

V

M

V

R

R

D

D

K

K

N

N

D

D

N

N

C

C

V

I

I

I

I

V

C

C

S

S

I

I

E

E

N

N

L

F

D

D

P

A

M
|
M

G
|
G

V
x
I

H
|
H

T
|
T

G

G

D

D

S

S

I

I

T

T

I

V

A

A

P

P

A

A

M

Q

T

T

L

L

S

T

D

D

R

K

E

E

Y

Y

Q

Q

I

I

L

M

R

R

N

N

Y

A

S

S

I

M

A

A

V

V

M

L

R

R

E

E

I

I

G

G

V

V

E

E

T

T

G

G

S

S

N

N

V

V

Q
|
Q

F

F

S

A

Q

V

N

N

P

P

K

K

T

N

G

G

E

R

F

L

Y

I

V

V

I
|
I

E
|
E

M

M

N
|
N

P

P

R
|
R

V

V

S

S

R
|
R

S

S

A

A

L

L

A

A

S

S

K

K

A

A

T

T

G

G

F

F

P

P

I

I

A

A

K

K

I

V

A

A

K

K

L

L

A

A

V

V

G

G

Y

Y

T

T

L

L

D

D

E

E

L

L

P

M

N

N

D
|
D

I

I

T

T

-

G

K

G

K

R

T

T

P

P

A

A

S

S

F

F

E

E

P

P

T

S

I

I

D

D

Y

Y

V

V

V

T

K

K

I

I

P

P

R

R

F

F

D

N

F

F

A

E

K

K

F

F

P

A

E

G

A

A

D

N

P

D

T

R

L

L

T

T

T
|
T

M

Q

M

M

K

K

S

S

V

V

G

G

E

E

V

V

M

M

A

A

I

I

G

G

R

R

T

T

F

Q

K

Q

E

E

S

S

L

L

Q

Q

K

K

A

A

L

L

R

R

S

G

L

L

E

E

L

V

G

G

R

A

Y

T

G

G

F

F

-

D

-

P

Y

K

M

V

G

S

L

L

E

D

N

D

I

P

D

E

E

A

N

L

T

T

-

K

L

I

K

R

E

R

K

E

I

L

I

K

K

D

P

A

N

G

P

A

D

D

R

R

L

I

W

W

Y

Y

I

I

A

A

E

D

A

A

F

F

R

R

K

A

G

G

Y

L

T

S

V

V

E

D

E

G

I

V

N

F

K

N

L

L

S

T

H

N

I

I

D

D

K

R

W

W

F

F

L

L

N

V

Q

Q

I

I

K

E

E

E

I

L

I

V

D

R

Y

L

E

E

L

E

Y

K

L

V

S

A

T

E

K

V

T

G

I

I

D

T

T

G

I

L

T

N

D

A

Q

D

E

F

L

L

E

R

K

Q

A

L

K

K

Q

R

W

K

G

G

F

F

S

A

D

D

R

A

E

R

L

L

A

A

R

K

L

L

L

A

K
x
G

T

V

T

R

E

E

E

A

E

E

V

I

R

R

K

K

R

L

K

R

-

D

-

Q

-

Y

-

D

I

L

I

H

V

P

S

V

Y

Y

K

K

V

R

V

V

D

D

T

T

C

C

A

A

E

E

F

F

E

A

A

T

F

D

T

T

P

A

Y

Y

Y

M

Y

Y

S

S

S

T

Y

Y

E

E

active site: K202 (≠ H202), D338 (= D338), G507 (≠ K503)
binding adenosine-5'-diphosphate: R129 (= R129), I167 (= I167), R169 (= R169), M174 (≠ L174), G175 (= G175), G176 (= G176), L210 (≠ V210), E215 (= E215), M240 (= M240), G241 (= G241), I242 (≠ V242), H243 (= H243), T244 (= T244), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (= T375)
binding manganese (ii) ion: Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301)
binding phosphate ion: G175 (= G175), H243 (= H243), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)

Sites not aligning to the query:

active site: 634, 754, 886
binding adenosine-5'-diphosphate: 715, 718, 740, 741, 746, 770, 771, 772, 773, 826
binding manganese (ii) ion: 814, 826
binding L-ornithine: 768, 776, 877, 892, 1025, 1026, 1027

1ce8A Carbamoyl phosphate synthetase from escherichis coli with complexed with the allosteric ligand imp (see paper)
62% identity, 97% coverage: 1:540/559 of query aligns to 1:548/1058 of 1ce8A

query
sites
1ce8A

M

M

P

P

K

K

R

R

T

T

D

D

I

I

E

K

T

S

I

I

L

L

L

I

I

L

G

G

S

A

G

G

P

P

I

I

V

V

I

I

G

G

Q

Q

A

A

A

C

E

E

F

F

D

D

Y

Y

S

S

G

G

T

A

Q

Q

G

A

A

C

K

K

A

A

L

L

K

R

E

E

G

G

Y

Y

R

R

V

V

I

I

L

N

V

V

N

N

S

S

N

N

P

P

A

A

T

T

I

I

M

M

T

T

D

D

P

P

N

E

I

M

A

A

D

D

R

A

T

T

Y

Y

I

I

E

E

P

P

L

I

I

H

T

W

P

E

V

V

L

V

E

R

K

K

I

I

E

E

K

K

E

E

R

R

P

P

D

D

A

A

I

V

L

L

P

P

T

T

L

M

G

G

Q

Q

T

T

A

A

L

L

N

N

L

C

A

A

I

L

D

E

L

L

Y

E

E

R

K

Q

G

G

I

V

L

L

D

E

K

E

Y

F

N

G

V

V

E

T

L

M

I

I

G

G

A

A

K

T

Y

A

E

D

V

A

I

I

K

D

K

K

A

A

E

E

D

D

R
|
R

D

R

L

R

F

F

K

D

K

V

A

A

M

M

E

K

N

K

I

I

G

G

L

L

S

E

M

T

P

A

K

R

S

S

A

G

V

I

V

A

K

H

S

T

V

M

A

E

E

E

A

A

E

L

E

A

V

V

I

A

D

A

W

D

I

V

G

G

F

F

P

P

V

C

I
|
I

I

I

R
|
R

P

P

S

S

F

F

T

T

L
x
M

G
|
G

T

S

G

G

S

G

I

I

A

A

Y

Y

N

N

R

R

E

E

F

F

Y

E

P

E

K

I

V

C

K

A

A

R

G

G

L

L

D

D

A

L

S

S

P

P

I

T

H
x
K

E

E

V

L

L

L

L

I

E

D

E

E

S

S

V
x
L

I
|
I

G

G

W

W

K

K

E
|
E

F

Y

E

E

M

M

E

E

V

V

M

V

R

R

D

D

K

K

N

N

D

D

N

N

C

C

V

I

I

I

I

V

C

C

S

S

I

I

E

E

N

N

L

F

D

D

P

A

M
|
M

G
|
G

V
x
I

H
|
H

T

T

G

G

D

D

S

S

I

I

T

T

I

V

A

A

P

P

A

A

M

Q

T

T

L

L

S

T

D

D

R

K

E

E

Y

Y

Q

Q

I

I

L

M

R

R

N

N

Y

A

S

S

I

M

A

A

V

V

M

L

R

R

E

E

I

I

G

G

V

V

E

E

T

T

G

G

S

S

N
|
N

V

V

Q
|
Q

F

F

S

A

Q

V

N

N

P

P

K

K

T

N

G

G

E

R

F

L

Y

I

V

V

I
|
I

E
|
E

M

M

N
|
N

P

P

R
|
R

V

V

S

S

R
|
R

S
|
S

S

S

A

A

L

L

A

A

S

S

K

K

A

A

T

T

G

G

F

F

P

P

I

I

A

A

K

K

I

V

A

A

K

K

L

L

A

A

V

V

G

G

Y

Y

T

T

L

L

D

D

E

E

L

L

P

M

N

N

D
|
D

I

I

T

T

-

G

K

G

K

R

T

T

P

P

A

A

S

S

F

F

E

E

P

P

T

S

I

I

D

D

Y

Y

V

V

V

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active site: R129 (= R129), R169 (= R169), M174 (≠ L174), G176 (= G176), K202 (≠ H202), E215 (= E215), H243 (= H243), N283 (= N283), Q285 (= Q285), E299 (= E299), N301 (= N301), R303 (= R303), S307 (= S307), D338 (= D338), G507 (≠ K503)
binding adenosine-5'-diphosphate: R129 (= R129), I167 (= I167), R169 (= R169), M174 (≠ L174), G176 (= G176), L210 (≠ V210), I211 (= I211), E215 (= E215), M240 (= M240), G241 (= G241), I242 (≠ V242), H243 (= H243), Q285 (= Q285), I298 (= I298), E299 (= E299), T376 (= T375)
binding manganese (ii) ion: M174 (≠ L174), Q285 (= Q285), E299 (= E299), E299 (= E299), N301 (= N301)
binding L-ornithine: R528 (≠ V520), A537 (≠ E529), T538 (≠ A530)
binding phosphate ion: M174 (≠ L174), G175 (= G175), H243 (= H243), E299 (= E299), N301 (= N301), R303 (= R303), R306 (= R306)

Sites not aligning to the query:

active site: 634, 715, 746, 754, 814, 826, 828, 833, 886
binding adenosine-5'-diphosphate: 715, 740, 741, 746, 770, 771, 772, 773, 774, 826
binding inosinic acid: 933, 939, 959, 961, 962, 978, 979, 986, 1000, 1001, 1002, 1010, 1011, 1013
binding manganese (ii) ion: 814, 826
binding L-ornithine: 552, 554, 768, 776, 877, 892, 1025, 1026, 1027

P20054 Multifunctional protein pyr1-3; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Dictyostelium discoideum (Social amoeba)
48% identity, 97% coverage: 7:546/559 of query aligns to 404:942/2225 of P20054

query
sites
P20054

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