SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028989447.1 NCBI__GCF_000423825.1:WP_028989447.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A1P6 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
71% identity, 99% coverage: 8:471/471 of query aligns to 6:469/469 of P0A1P6

query
sites
P0A1P6

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E130 (= E132) binding Mn(2+)
E132 (= E134) binding Mn(2+)
E208 (= E210) binding ATP
E213 (= E215) binding Mn(2+)
E221 (= E223) binding Mn(2+)
NG 265:266 (= NG 267:268) binding L-glutamate
H270 (= H272) binding Mn(2+)
HMS 272:274 (= HMS 274:276) binding ATP
R322 (= R324) binding L-glutamate
E358 (= E360) binding Mn(2+)
R360 (= R362) binding L-glutamate

P0A9C5 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Escherichia coli (strain K12) (see 3 papers)
70% identity, 99% coverage: 8:471/471 of query aligns to 6:469/469 of P0A9C5

query
sites
P0A9C5

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Y398 (= Y400) modified: O-AMP-tyrosine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1fpyA Crystal structure of glutamine synthetase from salmonella typhimurium with inhibitor phosphinothricin (see paper)
71% identity, 99% coverage: 8:471/471 of query aligns to 5:468/468 of 1fpyA

query
sites
1fpyA

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|
E

A

A

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

F

V

V

V

T

A

N

S

P

P

K

K

A

A

R

R

R
|
R

I

I

E
|
E

V

V

R
|
R

F

F

P

P

D

D

S

P

T

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

M

L

M

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

E

E

A

P

M

M

D

D

K

K

D

N

L

L

Y

Y

D

D

L

L

P

P

A

P

E

E

E

E

K

A

K

K

S

E

I

I

P

P

Q

Q

V

V

C

A

G

G

S

S

L

L

S

E

E

E

A

A

L

L

D

N

A

A

L

L

D

D

A

L

D

D

R

R

A

E

F

F

L

L

T

K

K

A

G

G

G

G

V

V

F

F

T

T

D

D

S

E

M

A

L

I

D

D

A

A

F

Y

I

I

E

A

L

L

K

R

R

R

A

E

E

E

V

D

Q

D

E

R

V

V

N

R

M

M

T

T

T

P

H

H

P

P

V

V

E

E

M

F

R

E

M

L

Y

Y

Y

Y

S

S

L

V

binding adenosine-5'-diphosphate: G127 (= G130), E129 (= E132), E207 (= E210), T223 (≠ V226), F225 (= F228), H271 (= H274), S273 (= S276), R355 (= R358), E357 (= E360)
binding manganese (ii) ion: E129 (= E132), E131 (= E134), E212 (= E215), E220 (= E223), H269 (= H272), E357 (= E360)
binding phosphinothricin: E131 (= E134), E212 (= E215), G265 (= G268), H269 (= H272), R321 (= R324), E327 (= E330), R359 (= R362)

1f1hA Crystal structure of glutamine synthetase from salmonella typhimurium with thallium ions (see paper)
71% identity, 99% coverage: 8:471/471 of query aligns to 5:468/468 of 1f1hA

query
sites
1f1hA

V

V

F

L

K

T

L

M

I

L

E

N

E

E

N

H

D

E

V

V

K

K

F

F

I

V

D

D

L

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

K

K

E

E

Q

Q

H

H

V

V

S

T

M

I

P

P

A

A

H

H

A

Q

L

V

D

N

E

A

G

E

A

F

F

F

E

E

E

E

G

G

K

K

A

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

K

H

G

I

I

N

N

E

E

S

S

D

D

M

M

I

V

L

L

K

M

L

P

D

D

P

A

S

S

S

T

A

A

V

V

M

I

D

D

P

P

F

F

M

F

D

A

E

D

N

S

T

T

L

L

I

I

I

I

R

R

C

C

D

D

I

I

I

L

E

E

P

P

A

G

T

T

G

L

E

Q

G

G

Y

Y

E

D

R

R

D

D

P

P

R

R

S

S

I

I

A

A

K

K

R

R

A

A

E

E

A

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

A

A

D

D

T

T

A

V

Y

L

F

F

G

G

P

P

E
|
E

N

P

E
|
E

F

F

F

F

I

L

F

F

D

D

S

D

V

I

T

R

W

F

S

G

I

A

D

S

M

I

S

S

G

G

C

S

S

H

Y

V

K

A

I

I

D

D

S

D

Q

I

E

E

A

G

A

A

W

W

N

N

S

S

G

S

K

T

E

K

Y

Y

E

E

S

G

G

G

N

N

M

K

A

G

H

H

R

R

P

P

S

G

V

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

V

V

D

D

S

S

S

A

Q

Q

D

D

I

I

R

R

S

S

A

E

M

M

C

C

L

L

A

V

L

M

E

E

E

Q

M

M

G

G

I

L

K

V

V

V

E
|
E

V

A

H

H

H
|
H

H

H

E
|
E

V

V

A

A

T

T

A

A

N

G

Q

Q

N

N

E
|
E

I

V

G

A

V
x
T

A

R

F
|
F

G

N

T

T

L

M

T

T

R

K

K

K

A

A

D

D

E

E

V

I

Q

Q

I

I

L

Y

K

K

Y

Y

V

V

V

V

H

H

N

N

V

V

A

A

H

H

A

R

Y

F

G

G

H

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

M

V

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

M

M

S
|
S

L

L

A

A

K

K

D

N

G

G

K

T

N

N

L

L

F

F

S

S

G

G

D

D

G

K

Y

Y

G

A

G

G

M

L

S

S

E

E

L

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

A

A

K

K

A

A

L

I

N

N

A

A

I

L

T

A

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

Y

E

E

A

A

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

F

V

V

V

T

A

N

S

P

P

K

K

A

A

R

R

R
|
R

I

I

E
|
E

V

V

R

R

F

F

P

P

D

D

S

P

T

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

M

L

M

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

E

E

A

P

M

M

D

D

K

K

D

N

L

L

Y

Y

D

D

L

L

P

P

A

P

E

E

E

E

K

A

K

K

S

E

I

I

P

P

Q

Q

V

V

C

A

G

G

S

S

L

L

S

E

E

E

A

A

L

L

D

N

A

A

L

L

D

D

A

L

D

D

R

R

A

E

F

F

L

L

T

K

K

A

G

G

G

G

V

V

F

F

T

T

D

D

S

E

M

A

L

I

D

D

A

A

F

Y

I

I

E

A

L

L

K

R

R

R

A

E

E

E

V

D

Q

D

E

R

V

V

N

R

M

M

T

T

T

P

H

H

P

P

V

V

E

E

M

F

R

E

M

L

Y

Y

Y

Y

S

S

L

V

binding adenosine-5'-diphosphate: E129 (= E132), E207 (= E210), H210 (= H213), E220 (= E223), T223 (≠ V226), F225 (= F228), H271 (= H274), S273 (= S276), R355 (= R358)
binding manganese (ii) ion: E129 (= E132), E131 (= E134), E212 (= E215), E220 (= E223), H269 (= H272), E357 (= E360)

2lgsA Feedback inhibition of fully unadenylylated glutamine synthetase from salmonella typhimurium by glycine, alanine, and serine (see paper)
67% identity, 99% coverage: 8:471/471 of query aligns to 5:445/445 of 2lgsA

query
sites
2lgsA

V

V

F

L

K

T

L

M

I

L

E

N

E

E

N

H

D

E

V

V

K

K

F

F

I

V

D

D

L

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

K

K

E

E

Q

Q

H

H

V

V

S

T

M

I

P

P

A

A

H

H

A

Q

L

V

D

N

E

A

G

E

A

F

F

F

E

E

E

E

G

G

K

K

A

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

-

H

-

I

-

N

-

E

-

S

-

D

D

M

M

I

V

L

L

K

M

L

P

D

D

P

A

S

S

S

T

A

A

V

V

M

I

D

D

P

P

F

F

M

F

D

A

E

D

N

S

T

T

L

L

I

I

I

I

R

R

C

C

D

D

I

I

I

L

E

E

P

P

A

G

T

T

G

L

E

Q

G

G

Y

Y

E

D

R

R

D

D

P

P

R

R

S

S

I

I

A

A

K

K

R

R

A

A

E

E

A

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

A

A

D

D

T

T

A

V

Y

L

F

F

G

G

P

P

E
|
E

N

P

E
|
E

F

F

F

F

I

L

F

F

D

D

S

D

V

I

T

R

W

F

S

G

I

A

D

S

M

I

S

S

G

G

C

S

S

H

Y

V

K

A

I

I

D

D

S

D

Q

I

E

E

A

G

A

A

W

W

N

N

S

S

G

S

K

T

E

K

Y

Y

E

E

S

G

G

G

N

N

M

K

A

G

H

H

R

R

P

P

S

G

V

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

V

V

D

D

S

S

S

A

Q

Q

D

D

I

I

R

R

S

S

A

E

M

M

C

C

L

L

A

V

L

M

E

E

E

Q

M

M

G

G

I

L

K

V

V

V

E

E

V

A

H

H

H

H

H

H

E
|
E

V

V

A

A

T

T

A

A

N

G

Q

Q

N

N

E
|
E

I

V

G

A

V

T

A

R

F

F

G

N

T

T

L

M

T

T

R

K

K

K

A

A

D

D

E

E

V

I

Q

Q

I

I

L

Y

K

K

Y

Y

V

V

V

V

H

H

N

N

V

V

A

A

H

H

A

R

Y

F

G

G

H

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

M

V

F

G

G

D

D

N
|
N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

C

H

H

M

M

S

S

L

L

A

A

K

K

D

N

G

G

K

T

N

N

L

L

F

F

S

S

G

G

D

D

G

K

Y

Y

G

A

G

G

M

L

S

S

E

E

L

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

A

A

K

K

A

A

L

I

N

N

A

A

I

L

T

A

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

-

F

-

E

-

A

-

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

F

V

V

V

T

A

N

S

P

P

K

K

A

A

R

R

R

R

I

I

E
|
E

V

V

R
|
R

F

F

P

P

D

D

S

P

T

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

M

L

M

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

E

E

A

P

M

M

D

D

K

-

D

-

L

-

Y

-

D

-

L

-

P

-

A

-

E

-

E

-

K

-

K

-

S

-

I

I

P

P

Q

Q

V

V

C

A

G

G

S

S

L

L

S

E

E

E

A

A

L

L

D

N

A

A

L

L

D

D

A

L

D

D

R

R

A

E

F

F

L

L

T

K

K

A

G

G

G

G

V

V

F

F

T

T

D

D

S

E

M

A

L

I

D

D

A

A

F

Y

I

I

E

A

L

L

K

R

R

R

A

E

E

E

V

D

Q

D

E

R

V

V

N

R

M

M

T

T

T

P

H

H

P

P

V

V

E

E

M

F

R

E

M

L

Y

Y

Y

Y

S

S

L

V

binding glutamic acid: E125 (= E134), N258 (= N267), G259 (= G268), G261 (= G270), H263 (= H272), R315 (= R324), R349 (= R362)
binding manganese (ii) ion: E123 (= E132), E125 (= E134), E206 (= E215), E214 (= E223), H263 (= H272), E347 (= E360)

1lgrA Interactions of nucleotides with fully unadenylylated glutamine synthetase from salmonella typhimurium (see paper)
67% identity, 99% coverage: 8:471/471 of query aligns to 5:445/445 of 1lgrA

query
sites
1lgrA

V

V

F

L

K

T

L

M

I

L

E

N

E

E

N

H

D

E

V

V

K

K

F

F

I

V

D

D

L

L

R

R

F

F

T

T

D

D

T

T

K

K

G

G

K

K

E

E

Q

Q

H

H

V

V

S

T

M

I

P

P

A

A

H

H

A

Q

L

V

D

N

E

A

G

E

A

F

F

F

E

E

E

E

G

G

K

K

A

M

F

F

D

D

G

G

S

S

S

S

I

I

A

G

G

G

W

W

K

-

H

-

I

-

N

-

E

-

S

-

D

D

M

M

I

V

L

L

K

M

L

P

D

D

P

A

S

S

S

T

A

A

V

V

M

I

D

D

P

P

F

F

M

F

D

A

E

D

N

S

T

T

L

L

I

I

I

I

R

R

C

C

D

D

I

I

I

L

E

E

P

P

A

G

T

T

G

L

E

Q

G

G

Y

Y

E

D

R

R

D

D

P

P

R

R

S

S

I

I

A

A

K

K

R

R

A

A

E

E

A

D

Y

Y

L

L

K

R

S

A

T

T

G

G

I

I

A

A

D

D

T

T

A

V

Y

L

F

F

G

G

P

P

E
|
E

N

P

E
|
E

F

F

F

F

I

L

F

F

D

D

S

D

V

I

T

R

W

F

S

G

I

A

D

S

M

I

S

S

G

G

C

S

S

H

Y

V

K

A

I

I

D

D

S

D

Q

I

E

E

A

G

A

A

W

W

N

N

S

S

G

S

K

T

E

K

Y

Y

E

E

S

G

G

G

N

N

M

K

A

G

H

H

R

R

P

P

S

G

V

V

K

K

G

G

G

G

Y

Y

F

F

P

P

V

V

P

P

P

P

V

V

D

D

S

S

S

A

Q

Q

D

D

I

I

R

R

S

S

A

E

M

M

C

C

L

L

A

V

L

M

E

E

E

Q

M

M

G

G

I

L

K

V

V

V

E
|
E

V

A

H

H

H

H

H

H

E
|
E

V

V

A

A

T

T

A

A

N

G

Q

Q

N

N

E
|
E

I

V

G

A

V
x
T

A

R

F
|
F

G

N

T

T

L

M

T

T

R

K

K

K

A

A

D

D

E

E

V

I

Q

Q

I

I

L

Y

K

K

Y

Y

V

V

V

V

H

H

N

N

V

V

A

A

H

H

A

R

Y

F

G

G

H

K

T

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

I

M

V

F

G

G

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

C

H
|
H

M

M

S
|
S

L

L

A

A

K

K

D

N

G

G

K

T

N

N

L

L

F

F

S

S

G

G

D

D

G

K

Y

Y

G

A

G

G

M

L

S

S

E

E

L

Q

A

A

L

L

Y

Y

Y

Y

I

I

G

G

G

G

I

V

I

I

K

K

H

H

A

A

K

K

A

A

L

I

N

N

A

A

I

L

T

A

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

-

F

-

E

-

A

-

P

P

V

V

M

M

L

L

A

A

Y

Y

S

S

A

A

R

R

N

N

R

R

S

S

A

A

S

S

I

I

R

R

I

I

P

P

F

V

V

V

T

A

N

S

P

P

K

K

A

A

R

R

R
|
R

I

I

E
|
E

V

V

R

R

F

F

P

P

D

D

S

P

T

A

A

A

N

N

P

P

Y

Y

L

L

A

C

F

F

A

A

A

A

M

L

M

L

M

M

A

A

G

G

L

L

D

D

G

G

I

I

Q

K

N

N

K

K

I

I

H

H

P

P

G

G

E

E

A

P

M

M

D

D

K

-

D

-

L

-

Y

-

D

-

L

-

P

-

A

-

E

-

E

-

K

-

K

-

S

-

I

I

P

P

Q

Q

V

V

C

A

G

G

S

S

L

L

S

E

E

E

A

A

L

L

D

N

A

A

L

L

D

D

A

L

D

D

R

R

A

E

F

F

L

L

T

K

K

A

G

G

G

G

V

V

F

F

T

T

D

D

S

E

M

A

L

I

D

D

A

A

F

Y

I

I

E

A

L

L

K

R

R

R

A

E

E

E

V

D

Q

D

E

R

V

V

N

R

M

M

T

T

T

P

H

H

P

P

V

V

E

E

M

F

R

E

M

L

Y

Y

Y

Y

S

S

L

V

binding adenosine monophosphate: E201 (= E210), T217 (≠ V226), F219 (= F228), H265 (= H274), S267 (= S276), R345 (= R358)
binding manganese (ii) ion: E123 (= E132), E125 (= E134), E206 (= E215), E214 (= E223), H263 (= H272), E347 (= E360)

P77961 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Synechocystis sp. (strain PCC 6803 / Kazusa)
56% identity, 100% coverage: 1:469/471 of query aligns to 1:471/473 of P77961

query
sites
P77961

M

M

A

A

D

R

A

T

V

P

K

Q

D

E

V

V

F

L

K

K

L

W

I

I

E

Q

E

D

N

E

D

N

V

I

K

K

F

I

I

I

D

D

L

L

R

K

F

F

T

I

D

D

T

T

K

P

G

G

K

I

E

W

Q

Q

H

H

V

C

S

S

M

F

P

Y

A

Y

H

D

A

Q

L

L

D

D

E

E

G

N

A

S

F

F

E

T

E

E

G

G

K

I

A

P

F

F

D

D

G

G

S

S

S

S

I

I

A

R

G

G

W

W

K

K

H

A

I

I

N

N

E

E

S

S

D

D

M

M

I

C

L

M

K

V

L

P

D

D

P

P

S

N

S

T

A

A

V

T

M

I

D

D

P

P

F

F

M

C

D

K

E

E

N

P

T

T

L

L

I

S

I

M

R

I

C

C

D

S

I

I

I

K

E

E

P

P

A

R

T

T

G

G

E

E

G

W

Y

Y

E

N

R

R

D

D

P

P

R

R

S

T

I

I

A

A

K

A

R

K

A

A

E

V

A

E

Y

Y

L

L

K

R

S

G

T

T

G

G

I

I

A

A

D

D

T

T

A

V

Y

Y

F

F

G

G

P

P

E

E

N

A

E
|
E

F

F

F

F

I

L

F

F

D

D

S

D

V

I

T

R

W

F

S

G

I

Q

D

T

M

E

S

N

G

S

C

S

S

Y

Y

Y

K

F

I

A

D

D

S

S

Q

V

E

E

A

G

A

R

W

W

N

N

S

T

G

G

K

R

E

E

Y

E

E

E

S

G

G

G

N

N

M

L

A

G

H

Y

R

K

P

P

S

G

V

Y

K

K

G

Q

G

G

Y

Y

F

F

P

P

V

V

P

A

P

P

V

T

D

D

S

T

S

A

Q

Q

D

D

I

I

R

R

S

T

A

E

M

M

C

L

L

L

A

T

L

M

E

A

E

G

M

L

G

C

I

V

K

P

V

I

E

E

V

K

H

H

H

H

H

H

E
|
E

V

V

A

A

T

S

A

G

N

G

Q

Q

N

N

E
|
E

I

L

G

G

V

I

A

K

F

F

G

D

T

K

L

L

T

V

R

N

K

S

A

A

D

D

E

N

V

L

Q

M

I

I

L

Y

K

K

Y

Y

V

V

V

I

H

K

N

N

V

V

A

A

H

K

A

K

Y

Y

G

G

H

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

I

I

V

F

G

N

D

D

N

N

G

G

S

S

G

G

M

M

H

H

V

V

H

H

M

Q

S

S

L

L

A

W

K

K

D

D

G

G

K

Q

N

P

L

L

F

F

S

A

G

G

D

D

G

K

Y

Y

G

A

G

G

M

F

S

S

E

Q

L

M

A

G

L

L

Y

W

Y

Y

I

I

G

G

G

G

I

I

I

L

K

K

H

H

A

A

K

P

A

A

L

L

N

L

A

A

I

F

T

T

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

F

E

E

A

A

P

P

V

V

M

N

L

L

A

A

Y

Y

S

S

A

Q

R

G

N

N

R

R

S

S

A

A

S

S

I

V

R

R

I

I

P

P

F

L

V

S

-

G

T

G

N

N

P

P

K

K

A

A

R

K

R

R

I

L

E
|
E

V

F

R

R

F

C

P

P

D

D

S

A

T

T

A

S

N

N

P

P

Y

Y

L

L

A

A

F

F

A

A

A

A

M

M

M

L

M

C

A

A

G

G

L

I

D

D

G

G

I

I

Q

K

N

N

K

Q

I

I

H

D

P

P

G

G

E

E

A

P

M

L

D

D

K

V

D

D

L

I

Y

Y

D

D

L

L

P

S

A

P

E

E

E

E

K

L

K

A

S

K

I

I

P

P

Q

S

V

T

C

P

G

G

S

S

L

L

S

E

E

A

A

A

L

L

D

E

A

A

L

L

D

E

A

K

D

D

R

H

A

E

F

F

L

L

T

T

K

G

G

T

G

G

V

V

F

F

T

S

D

P

S

D

M

F

L

V

D

E

A

S

F

W

I

I

E

E

L

Y

K

K

-

L

R

D

A

N

E

E

V

V

Q

N

E

P

V

M

N

R

M

L

T

R

T

P

H

H

P

P

V

Y

E

E

M

F

R

S

M

L

Y

Y

Y

Y

E134 (= E134) binding Mg(2+)
E215 (= E215) binding Mg(2+)
E223 (= E223) binding Mg(2+)
E361 (= E360) binding Mn(2+)

3ng0A Crystal structure of glutamine synthetase from synechocystis sp. Pcc 6803
55% identity, 99% coverage: 6:469/471 of query aligns to 4:463/465 of 3ng0A

query
sites
3ng0A

K

Q

D

E

V

V

F

L

K

K

L

W

I

I

E

Q

E

D

N

E

D

N

V

I

K

K

F

I

I

I

D

D

L

L

R

K

F

F

T

I

D

D

T

T

K

P

G

G

K

I

E

W

Q

Q

H

H

V

C

S

S

M

F

P

Y

A

Y

H

D

A

Q

L

L

D

D

E

E

G

N

A

S

F

F

E

T

E

E

G

G

K

I

A

P

F

F

D
|
D

G

G

S

S

S

S

I

I

A

R

G

G

W

W

K

K

H

A

I

I

N

N

E

E

S

S

D

D

M

M

I

C

L

M

K

V

L

P

D

D

P

P

S

N

S

T

A

A

V

T

M

I

D

D

P

P

F

F

M

C

D

K

E

E

N

P

T

T

L

L

I

S

I

M

R

I

C

C

D

S

I

I

I

K

E

E

P

P

A

R

T

T

G

G

E

E

G

W

Y

Y

E

N

R

R

D

D

P

P

R

R

S

T

I

I

A

A

K

A

R

K

A

A

E

A

A

E

Y

Y

L

L

K

R

S

G

T

T

G

G

I

I

A

A

D

D

T

T

A

V

Y
|
Y

F

F

G

G

P

P

E
|
E

N

A

E
|
E

F

F

F

F

I

L

F

F

D

D

S

D

V

I

T

R

W

F

S

G

I

Q

D

T

M

E

S

N

G

S

C

S

S

Y

Y

Y

K

F

I

A

D

D

S

S

Q

V

E

E

A

G

A

R

W

W

N

N

S

T

G

G

K

R

E

E

Y

E

E

E

S

G

G

G

N

N

M

L

A

G

H

Y

R

K

P

P

S

G

V

Y

K

K

G

Q

G

G

Y

Y

F

F

P

P

V

V

P

A

P

P

V

T

D

D

S

T

S

A

Q

Q

D

D

I

I

R

R

S

T

A

E

M

M

C

L

L

L

A

T

L

M

E

A

E

A

M

F

G

G

I

V

K

P

V

I

E

E

V
x
K

H

H

H

H

H

H

E
|
E

V

V

A

A

T

S

A

G

N

G

Q

Q

N

N

E
|
E

I

L

G

G

V
x
I

A

K

F
|
F

G

D

T

K

L

L

T

V

R

N

K

S

A

A

D

D

E

N

V

L

Q

M

I

I

L

Y

K

K

Y

Y

V

V

V

I

H

K

N

N

V

V

A

A

H

K

A

K

Y

Y

G

G

H

K

T

T

A

V

T

T

F

F

M

M

P

P

K

K

P

P

I

I

V

F

G

N

D

D

N

N

G

G

S

S

G

G

M

M

H
|
H

V

V

H
|
H

M

Q

S
|
S

L

L

A

W

K

K

D

D

G

G

K

Q

N

P

L

L

F

F

S

A

G

G

D

D

G

K

Y

Y

G

A

G

G

M

F

S

S

E

Q

L

M

A

G

L

L

Y

W

Y

Y

I

I

G

G

G

G

I

I

I

L

K

K

H

H

A

A

K

P

A

A

L

L

N

L

A

A

I

F

T

T

N

N

P

P

S

T

T

T

N

N

S

S

Y

Y

K

K

R

R

L

L

V

V

P

P

G

G

F

F

E

E

A

A

P

P

V

V

M

N

L

L

A

A

Y

Y

S

S

A

Q

R

G

N

N

R
|
R

S

S

A

A

S

S

I

V

R
|
R

I

I

P

P

F

L

V

S

-

G

T

G

N

N

P

P

K

K

A

A

R

K

R
|
R

I

L

E
|
E

V

F

R
|
R

F

C

P

P

D

D

S

A

T

T

A

S

N

N

P

P

Y

Y

L

L

A

A

F

F

A

A

A

A

M

M

M

L

M

C

A

A

G

G

L

I

D

D

G

G

I

I

Q

K

N

N

K

Q

I

I

H

D

P

P

G

G

E

E

A

P

M

L

D

D

K

V

D

D

L

I

Y

-

D

-

L

-

P

-

A

-

E

-

E

E

K

L

K

A

S

K

I

I

P

P

Q

S

V

T

C

P

G

G

S

S

L

L

S

E

E

A

A

A

L

L

D

E

A

A

L

L

D

E

A

K

D

D

R

H

A

E

F

F

L

L

T

T

K

G

G

T

G

G

V

V

F

F

T

S

D

P

S

D

M

F

L

V

D

E

A

S

F

W

I

I

E

E

L

Y

K

K

-

L

R

D

A

N

E

E

V

V

Q

N

E

P

V

M

N

R

M

L

T

R

T

P

H

H

P

P

V

Y

E

E

M

F

R

S

M

L

Y

Y

Y

Y

active site: D51 (= D53), E130 (= E132), E132 (= E134), E213 (= E215), E221 (= E223), H270 (= H272), R340 (= R342), E359 (= E360), R361 (= R362)
binding phosphoaminophosphonic acid-adenylate ester: Y126 (= Y128), E130 (= E132), K209 (≠ V211), I224 (≠ V226), F226 (= F228), H272 (= H274), S274 (= S276), R340 (= R342), R345 (= R347), R357 (= R358)
binding manganese (ii) ion: E130 (= E132), E132 (= E134), E213 (= E215), E221 (= E223), H270 (= H272), E359 (= E360), R361 (= R362)

A0R079 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
52% identity, 99% coverage: 7:471/471 of query aligns to 8:478/478 of A0R079

query
sites
A0R079

D

D

V

I

F

F

K

K

L

L

I

I

E
x
K

E

D

N

E

D

N

V

V

K

E

F

Y

I

V

D

D

L

I

R

R

F

F

T

C

D

D

T

L

K

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K14 (≠ E13) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

2whiA Crystal structure of mycobacterium tuberculosis glutamine synthetase in complex with a purine analogue inhibitor and l-methionine-s- sulfoximine phosphate. (see paper)
52% identity, 99% coverage: 7:471/471 of query aligns to 6:476/476 of 2whiA

query
sites
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