SitesBLAST
Comparing WP_028989560.1 NCBI__GCF_000423825.1:WP_028989560.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
60% identity, 99% coverage: 3:391/393 of query aligns to 4:391/392 of P33221
- EL 22:23 (= EL 21:22) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E81) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R114) binding ATP
- K155 (= K155) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 160:165) binding ATP
- G162 (= G162) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ A179) modified: N6-acetyllysine
- EGVV 195:198 (≠ ESFV 195:198) binding ATP
- E203 (= E203) binding ATP
- E267 (= E267) binding Mg(2+)
- E279 (= E279) binding Mg(2+)
- D286 (= D286) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K355) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 362:363) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
60% identity, 99% coverage: 3:391/393 of query aligns to 3:388/389 of 1kjiA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R363)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E192 (= E195), V195 (= V198), E200 (= E203), Q222 (= Q225), E264 (= E267), F266 (= F269), E276 (= E279)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
60% identity, 99% coverage: 3:391/393 of query aligns to 3:388/389 of 1ez1A
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R363)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), E192 (= E195), V194 (≠ F197), V195 (= V198), F197 (= F200), E200 (= E203), Q222 (= Q225), E264 (= E267), F266 (= F269), E276 (= E279)
- binding glycinamide ribonucleotide: G20 (= G20), E21 (= E21), L22 (= L22), E81 (= E81), I82 (= I82), S160 (= S161), D283 (= D286), K352 (= K355), R359 (= R362), R360 (= R363)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
60% identity, 99% coverage: 3:391/393 of query aligns to 3:388/389 of 1eyzA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E267), E276 (= E279), D283 (= D286), T284 (= T287), R360 (= R363)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E192 (= E195), V195 (= V198), F197 (= F200), E200 (= E203), E264 (= E267), F266 (= F269), E276 (= E279)
- binding magnesium ion: E264 (= E267), E276 (= E279)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
60% identity, 99% coverage: 3:391/393 of query aligns to 3:385/386 of 1kjjA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E261 (= E267), E273 (= E279), D280 (= D286), T281 (= T287), R357 (= R363)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E189 (= E195), V192 (= V198), E197 (= E203), Q219 (= Q225), E261 (= E267), F263 (= F269), E273 (= E279)
- binding magnesium ion: E261 (= E267), E273 (= E279)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
60% identity, 99% coverage: 3:391/393 of query aligns to 3:385/386 of 1kj8A
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E261 (= E267), E273 (= E279), D280 (= D286), T281 (= T287), R357 (= R363)
- binding adenosine-5'-triphosphate: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E189 (= E195), V192 (= V198), F194 (= F200), E197 (= E203), Q219 (= Q225), G222 (= G228), E261 (= E267), F263 (= F269), E273 (= E279)
- binding glycinamide ribonucleotide: G20 (= G20), E21 (= E21), L22 (= L22), E81 (= E81), I82 (= I82), S160 (= S161), D280 (= D286), K349 (= K355), R356 (= R362)
- binding magnesium ion: E261 (= E267), E273 (= E279)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
59% identity, 99% coverage: 3:391/393 of query aligns to 3:387/388 of 1kjqA
- active site: E114 (= E115), K154 (= K155), E263 (= E267), E275 (= E279), D282 (= D286), T283 (= T287), R359 (= R363)
- binding adenosine-5'-diphosphate: R113 (= R114), I152 (≠ V153), K154 (= K155), E191 (= E195), V193 (≠ F197), V194 (= V198), F196 (= F200), E199 (= E203), Q221 (= Q225), F265 (= F269), E275 (= E279)
- binding magnesium ion: E263 (= E267), E275 (= E279)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
52% identity, 99% coverage: 3:391/393 of query aligns to 8:411/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
50% identity, 99% coverage: 3:391/393 of query aligns to 10:400/409 of 2dwcB
- active site: E265 (= E267), E277 (= E279), D284 (= D286), T285 (= T287), R372 (= R363)
- binding adenosine-5'-diphosphate: R120 (= R114), H159 (≠ V153), K161 (= K155), H190 (≠ F197), I191 (≠ V198), F193 (= F200), E196 (= E203), F267 (= F269), E277 (= E279)
3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
27% identity, 92% coverage: 28:390/393 of query aligns to 18:357/360 of 3ax6A
- active site: E231 (= E267), E244 (= E279), N251 (≠ D286), S252 (≠ T287), K330 (≠ R363)
- binding adenosine-5'-diphosphate: K101 (≠ R114), V136 (= V153), K138 (= K155), E164 (= E195), F166 (= F197), V167 (= V198), E172 (= E203), F233 (= F269), N243 (≠ S278)
3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 95% coverage: 10:381/393 of query aligns to 1:367/381 of 3v4sB
- binding adenosine-5'-triphosphate: R108 (= R114), K148 (= K155), Y154 (vs. gap), D155 (≠ S161), G156 (= G162), Q159 (= Q165), E183 (= E195), W185 (≠ F197), V186 (= V198), F188 (= F200), E191 (= E203), H214 (≠ E226), N217 (≠ D229), E256 (= E267), F258 (= F269), E269 (= E279)
- binding carbonate ion: R273 (= R283), H275 (= H285), N276 (≠ D286)
- binding magnesium ion: T105 (= T111), E111 (≠ R118), E256 (= E267), E269 (= E279), L270 (≠ V280)
4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 94% coverage: 12:381/393 of query aligns to 2:366/380 of 4dlkA