SitesBLAST

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
36% identity, 54% coverage: 68:174/199 of query aligns to 72:181/185 of 3nz2J

query
sites
3nz2J

G

G

R

K

N

T

L

I

F

R

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I

G

G

A

D

E

H

C

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x
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x
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L

binding acetyl coenzyme *a: G104 (≠ N100), Y110 (≠ L106), H114 (= H110), W138 (= W131), G140 (≠ A133), A158 (≠ G151), A159 (= A152), N164 (≠ T157), G174 (≠ V167), T176 (≠ N169), P177 (= P170)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
36% identity, 54% coverage: 68:174/199 of query aligns to 69:178/183 of 3nz2C

query
sites
3nz2C

G

G

R

K

N

T

L

I

F

R

I

I

G

G

A

D

E

H

C

T

A

F

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G

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N

V

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Y

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G

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A

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N

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L

binding acetyl coenzyme *a: Y107 (≠ L106), H111 (= H110), G137 (≠ A133), A155 (≠ G151), A156 (= A152), N161 (≠ T157), G171 (≠ V167), T173 (≠ N169), P174 (= P170), L178 (= L174)
binding magnesium ion: N157 (≠ G153), T173 (≠ N169)

Sites not aligning to the query:

binding acetyl coenzyme *a: 179

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
36% identity, 54% coverage: 68:174/199 of query aligns to 62:171/176 of 3ectA

query
sites
3ectA

G

G

R

K

N

T

L

I

F

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I

G

G

A

D

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G

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L

binding calcium ion: G138 (= G141), D156 (= D159)

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
40% identity, 51% coverage: 73:173/199 of query aligns to 31:139/290 of 4mzuB

query
sites
4mzuB

I

I

G

G

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R

E

N

C

C

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N

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x
C

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x
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F

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A

G

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K

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A

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binding coenzyme a: F72 (vs. gap), T80 (= T120), N81 (vs. gap), D82 (vs. gap), K83 (vs. gap), P85 (vs. gap), G99 (≠ A133), A100 (≠ E134), G117 (= G151), A118 (= A152), T123 (= T157), K124 (= K158), I133 (≠ V167), R138 (= R172)
binding magnesium ion: G58 (= G102), N76 (≠ A116)
binding thymine: C38 (≠ V80), A39 (≠ G81)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
38% identity, 51% coverage: 73:173/199 of query aligns to 31:140/294 of 4mzuF

query
sites
4mzuF

I

I

G

G

A

R

E

N

C

C

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N

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x
C

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binding coenzyme a: N81 (≠ A116), D82 (≠ W117), K83 (≠ R118), G100 (≠ A133), A101 (≠ E134), L106 (= L139), G118 (= G151), A119 (= A152), T124 (= T157), K125 (= K158), I134 (≠ V167), N136 (= N169), R139 (= R172)
binding thymine: C38 (≠ V80)

Sites not aligning to the query:

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
32% identity, 58% coverage: 68:183/199 of query aligns to 78:194/201 of 1krvA

query
sites
1krvA

G

G

R

R

N

N

L

F

F
x
Y

I

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G
x
N

A

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N

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N

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K

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F72), N84 (≠ G74), V90 (= V80), D92 (≠ E82), M126 (= M119)
binding coenzyme a: A104 (≠ N100), S110 (vs. gap), T112 (= T107), W138 (= W131), G140 (≠ A133), S141 (≠ E134), N146 (≠ L139), A159 (= A152), P177 (= P170), R179 (= R172), R182 (vs. gap)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
32% identity, 58% coverage: 68:183/199 of query aligns to 78:194/201 of 1kruA

query
sites
1kruA

G

G

R

R

N

N

L

F

F
x
Y

I

A

G
x
N

A

F

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N

C

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V

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x
D

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L

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G

A
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A

G

G

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T

K

K

D

D

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P

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N

A

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x
R

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N

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binding coenzyme a: H114 (≠ E109), W138 (= W131), G140 (≠ A133), S141 (≠ E134), G158 (= G151), A159 (= A152), P177 (= P170), R182 (vs. gap)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ F72), Y82 (≠ F72), N84 (≠ G74), V90 (= V80), D92 (≠ E82), L102 (≠ V98), H114 (≠ E109), M126 (= M119)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 58% coverage: 68:183/199 of query aligns to 79:195/203 of P07464

query
sites
P07464

G

G

R

R

N

N

L

F

F

Y

I

A

G
x
N

A

F

E

N

C

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A

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H

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W

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G

G

A
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A

G

G

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T
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T

K
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K

D

D

V

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P

P

D

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F

N

A

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V

A

A

V

A

D

G

N

V

P

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A

C

R
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R

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x
R

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N

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K

N85 (≠ G74) binding in other chain; binding in other chain
D93 (≠ E82) binding substrate
H115 (≠ E109) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ E134) binding in other chain
A160 (= A152) binding in other chain
TK 165:166 (= TK 157:158) binding acetyl-CoA
R180 (= R172) binding acetyl-CoA
R183 (vs. gap) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
38% identity, 53% coverage: 68:172/199 of query aligns to 108:217/260 of 3fsbA

query
sites
3fsbA

G

G

R

N

N

N

L

V

F

K

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G
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G

A

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G

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H

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Y

I

I

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G

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N

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Y

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N

L

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H
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H

A

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N

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x
F

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N

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A

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A

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P

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A

A

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G
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G

A
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A

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G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

D

K

F

E

A

T

I

V

A

V

V
|
V

D

G

N

N

P

P

A

A

R

R

binding coenzyme a: F150 (vs. gap), A178 (= A133), A179 (≠ E134), G196 (= G151), A197 (= A152), V212 (= V167)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (= G74), H132 (= H88), F150 (vs. gap)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 241, 243

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
33% identity, 58% coverage: 68:183/199 of query aligns to 78:194/200 of 1krrA

query
sites
1krrA

G

G

R

R

N

N

L

F

F

Y

I

A

G
x
N

A

F

E

N

C

L

A

T

I

I

V

V

G

D

E

D

V

Y

R

T

I

-

A

-

L

-

H

-

A

-

P

-

V

V

R

T

I

I

G

G

D

D

R

N

V

V

V

L

I
|
I

N
x
A

D
x
P

G

N

V

V

R

T

I

L

-

S

L

V

T
|
T

G

G

E
x
H

-

P

-

V

H

H

D

H

V

E

C

L

D

R

P

K

A

N

W

G

R

E

M

M

T

Y

A

S

A

F

P

P

I

I

R

T

I

I

E

G

D

N

Y

N

A

V

W

W

V

I

A
x
G

E
x
S

G

H

A

V

I

V

I

I

L
x
N

P

P

G

G

V

V

R

T

I

I

G

G

K

D

G

N

A

S

V

V

V

I

G
|
G

A
|
A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

I

P

P

D

P

F

N

A

V

I

V

A

A

V
x
A

D

G

N

V

P
|
P

A

C

R

R

I

V

L

I

-
x
R

-

E

-

I

-

N

D

D

K

R

K

D

R

K

C

H

T

Y

R

Y

F

F

R

K

binding acetyl coenzyme *a: N84 (≠ G74), I103 (= I99), A104 (≠ N100), P105 (≠ D101), T112 (= T107), H114 (≠ E109), G140 (≠ A133), S141 (≠ E134), N146 (≠ L139), G158 (= G151), A159 (= A152), A174 (≠ V167), P177 (= P170), R182 (vs. gap)

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
38% identity, 53% coverage: 68:172/199 of query aligns to 108:217/259 of 3fscA

query
sites
3fscA

G

G

R

N

N

N

L

V

F
x
K

I

I

G
|
G

A
x
T

E

L

C

S

A

D

I

I

V

Q

G

H

E

H

V

V

R

Y

I

I

A

G

-

N

-

Y

-

V

-

N

L

I

H
|
H

A
x
S

P

N

V

V

R

F

I

V

G

G

D

E

R

K

V

S

V

I

I

I

N

K

D

D

G

F

V

V

-

W

-

L

-
x
F

-

P

-

H

R

V

I

V

L

L

T

T

G

-

E

-

H

N

D

D

V

P

C

T

D

P

P

P

A

S

W

N

R

E

M

L

T

L

A

G

A

-

P

-

I

V

R

T

I

I

E

E

D

L

Y

F

A

A

W

V

V

I

A
|
A

E
x
A

G

R

A

S

I

V

L

L

P

P

G

G

V

I

R

H

I

I

G

N

K

E

G

D

A

A

V

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

D

K

F

E

A

T

I

V

A

V

V
|
V

D

G

N

N

P

P

A

A

R

R

binding coenzyme a: F150 (vs. gap), A178 (= A133), A179 (≠ E134), G196 (= G151), A197 (= A152), V212 (= V167)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ F72), G114 (= G74), T115 (≠ A75), H132 (= H88), S133 (≠ A89)

Sites not aligning to the query:

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
38% identity, 53% coverage: 68:172/199 of query aligns to 108:217/259 of 3fs8A

query
sites
3fs8A

G

G

R

N

N

N

L

V

F

K

I

I

G

G

A

T

E

L

C

S

A

D

I

I

V

Q

G

H

E

H

V

V

R

Y

I

I

A

G

-

N

-

Y

-

V

-

N

L

I

H

H

A

S

P

N

V

V

R

F

I

V

G

G

D

E

R

K

V

S

V

I

I

I

N

K

D

D

G

F

V

V

-

W

-

L

-
x
F

-
x
P

-

H

R

V

I

V

L

L

T

T

G

-

E

-

H

N

D

D

V

P

C

T

D

P

P

P

A

S

W

N

R

E

M

L

T

L

A

G

A

-

P

-

I

V

R

T

I

I

E

E

D

L

Y

F

A

A

W

V

V

I

A
|
A

E
x
A

G

R

A

S

I

V

L

L

P

P

G

G

V

I

R

H

I

I

G

N

K

E

G

D

A

A

V

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

K

K

D

D

V

V

P

P

D

K

F

E

A

T

I

V

A

V

V
|
V

D

G

N

N

P

P

A

A

R

R

binding acetyl coenzyme *a: F150 (vs. gap), P151 (vs. gap), A178 (= A133), A179 (≠ E134), G196 (= G151), A197 (= A152), V212 (= V167)

Sites not aligning to the query:

7l7yAAA Putative acetyl transferase protein (see paper)
46% identity, 34% coverage: 109:175/199 of query aligns to 140:217/217 of 7l7yAAA

query
sites
7l7yAAA

E

E

H

H

D

D

V

-

C

C

D

I

P

I

A

G

W

D

R

Y

M

V

T

T

A

F

A

A

P

P

-

R

-

V

-

S

-

C

-

N

-

G

-

N

I

I

R

H

I

I

E

H

D

D

Y

H

A

A

W
x
Y

V

I

A
x
G

E
x
T

G

G

A

A

I

V

I

I

L

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

R

I

I

I

G

G

K

K

G

G

A

A

V

I

V

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

F

G

A

A

I

V

A

V

V
x
I

D

G

N

N

P

P

A

A

R

R

I

L

L

L

D

N

binding acetyl coenzyme *a: Y168 (≠ W131), G170 (≠ A133), T171 (≠ E134), G193 (= G151), M194 (≠ A152), I209 (≠ V167)

Sites not aligning to the query:

binding uridine-5'-diphosphate: 10, 12, 39, 40, 73, 74

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
37% identity, 31% coverage: 121:182/199 of query aligns to 224:301/307 of A1ADJ6

query
sites
A1ADJ6

A

A

A

K

P

D

I

I

R

I

I

I

E

S

D

S

Y

Y

A

V

W
|
W

V

V

A

G

E

R

G

N

A

V

I

S

I

I

L

M

P

K

G

G

V

V

R

S

I

V

G

G

K

S

G

G

A

S

V

V

V

I

G

G

A

Y

G

G

A

S

V

I

V

V

T

T

K

K

D

D

V

V

P

P

D

S

F

M

A

C

I

A

A

A

V

A

D

G

N

N

P

P

A

A

R

K

I

I

L

I

-

K

-

R

-

N

-

I

-

W

-

A

-

R

-

T

-

D

-

K

-

A

-

E

-

L

-

I

-

S

D

D

K

D

K

K

R

R

C

C

T

S

R

S

F

Y

W234 (= W131) mutation to A: Loss of activity.

Sites not aligning to the query:

210 H→A: Loss of activity.

7l82AAA Putative acetyl transferase protein (see paper)
46% identity, 33% coverage: 109:174/199 of query aligns to 140:216/216 of 7l82AAA

query
sites
7l82AAA

E

E

H

H

D

D

V

-

C

C

D

I

P

I

A

G

W

D

R

Y

M

V

T

T

A

F

A

A

P

P

-

R

-

V

-

S

-

C

-

N

-

G

-

N

I

I

R

H

I

I

E

H

D

D

Y

H

A

A

W
x
Y

V

I

A

G

E
x
T

G

G

A

A

I

V

I

I

L

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

R

I

I

I

G

G

K

K

G

G

A

A

V

I

V

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

F

G

A

A

I

V

A

V

V
x
I

D

G

N

N

P

P

A

A

R

R

I

L

L

L

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: Y168 (≠ W131), T171 (≠ E134), G193 (= G151), M194 (≠ A152), I209 (≠ V167)

Sites not aligning to the query:

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: 10, 12, 39, 40, 73, 74

7l81AAA Putative acetyl transferase protein (see paper)
46% identity, 33% coverage: 109:174/199 of query aligns to 140:216/216 of 7l81AAA

query
sites
7l81AAA

E

E

H

H

D

D

V

-

C

C

D

I

P

I

A

G

W

D

R

Y

M

V

T

T

A

F

A
|
A

P

P

-

R

-

V

-

S

-

C

-

N

-

G

-

N

I

I

R

H

I

I

E

H

D

D

Y

H

A

A

W
x
Y

V

I

A

G

E
x
T

G

G

A

A

I

V

I

I

L

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

R

I

I

I

G

G

K

K

G

G

A

A

V

I

V

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

F

G

A

A

I

V

A

V

V
x
I

D

G

N

N

P

P

A

A

R

R

I

L

L

L

binding coenzyme a: A152 (= A122), Y168 (≠ W131), T171 (≠ E134), G193 (= G151), M194 (≠ A152), I209 (≠ V167)

Sites not aligning to the query:

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: 10, 12, 39, 40, 73, 74

7l7zAAA Putative acetyl transferase protein (see paper)
46% identity, 33% coverage: 109:174/199 of query aligns to 140:216/216 of 7l7zAAA

query
sites
7l7zAAA

E

E

H

H

D

D

V

-

C

C

D

I

P

I

A

G

W

D

R

Y

M

V

T

T

A

F

A

A

P

P

-

R

-

V

-

S

-

C

-

N

-

G

-

N

I

I

R

H

I

I

E

H

D

D

Y

H

A

A

W
x
Y

V

I

A

G

E
x
T

G

G

A

A

I

V

I

I

L

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

V

L

R

I

I

I

G

G

K

K

G

G

A

A

V

I

V

V

G
|
G

A
x
M

G

G

A

A

V

V

T

T

K

K

D

E

V

V

P

P

D

A

F

G

A

A

I

V

A

V

V
x
I

D

G

N

N

P

P

A

A

R

R

I

L

L

L

binding coenzyme a: Y168 (≠ W131), T171 (≠ E134), G193 (= G151), M194 (≠ A152), I209 (≠ V167)

Sites not aligning to the query:

binding UDP-di-N-acetyl-alpha-bacillosamine: 10, 12, 39, 40, 73, 74

Query Sequence

>WP_028989986.1 NCBI__GCF_000423825.1:WP_028989986.1
MSSNLVYLWEHRERPKVLSPAWSKSWAKRVLHLRALLRANWRRAKLEAAGVQLGPMTEIG
ELKLNGPGRNLFIGAECAIVGEVRIALHAPVRIGDRVVINDGVRILTGEHDVCDPAWRMT
AAPIRIEDYAWVAEGAIILPGVRIGKGAVVGAGAVVTKDVPDFAIAVDNPARILDKKRCT
RFRYSPVRFLATYEAWLGH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory