SitesBLAST
Comparing WP_028990131.1 NCBI__GCF_000423825.1:WP_028990131.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8c0zE Cryoem structure of a tungsten-containing aldehyde oxidoreductase from aromatoleum aromaticum (see paper)
30% identity, 51% coverage: 6:416/810 of query aligns to 2:422/424 of 8c0zE
Q8U1K9 NAD(P)H:rubredoxin oxidoreductase; NROR; Rubredoxin--NAD(P)(+) reductase; EC 1.18.1.4 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
30% identity, 45% coverage: 6:371/810 of query aligns to 2:343/359 of Q8U1K9
- G11 (≠ A15) binding FAD
- DK 29:30 (≠ GA 37:38) binding FAD
- KP 38:39 (≠ RI 46:47) binding FAD
- A75 (≠ I83) binding FAD
- A103 (≠ S112) binding FAD
- R120 (= R131) binding FAD
- D260 (≠ E278) binding FAD
1xhcA Nadh oxidase /nitrite reductase from pyrococcus furiosus pfu-1140779- 001
31% identity, 41% coverage: 5:336/810 of query aligns to 1:317/346 of 1xhcA
- active site: P10 (≠ M14), K38 (≠ R46), P39 (≠ I47), F145 (≠ L156), E149 (= E160), M276 (≠ F294)
- binding flavin-adenine dinucleotide: V6 (≠ I10), G7 (= G11), G9 (= G13), P10 (≠ M14), G11 (≠ A15), D29 (≠ G37), K30 (≠ A38), K38 (≠ R46), P39 (≠ I47), E74 (≠ K82), A75 (≠ I83), A100 (= A109), T101 (= T110), G102 (= G111), L119 (≠ Y130), R120 (= R131), F145 (≠ L156), I146 (≠ L157), E149 (= E160), G259 (= G277), D260 (≠ E278), G270 (= G288), T271 (≠ L289), A272 (≠ V290)
Q9AL95 NADH-rubredoxin oxidoreductase; NROR; NADH:rubredoxin oxidoreductase; EC 1.18.1.1 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W) (see paper)
28% identity, 48% coverage: 6:393/810 of query aligns to 5:371/379 of Q9AL95
- C26 (≠ A27) modified: Disulfide link with 286
- NSE 33:35 (≠ GAE 37:39) binding FAD
- R42 (= R46) binding FAD
- A79 (≠ I83) binding FAD
- Y125 (≠ R131) binding FAD
- C137 (≠ S143) modified: Disulfide link with 216
- C216 (≠ L235) modified: Disulfide link with 137
- D259 (≠ E278) binding FAD
- C286 (≠ Y307) modified: Disulfide link with 26
3kljA Crystal structure of nadh:rubredoxin oxidoreductase from clostridium acetobutylicum (see paper)
28% identity, 48% coverage: 6:393/810 of query aligns to 4:370/378 of 3kljA
- active site: P12 (≠ M14), R41 (= R46), P42 (≠ I47), N274 (≠ F294)
- binding flavin-adenine dinucleotide: L8 (≠ I10), G9 (= G11), G11 (= G13), P12 (≠ M14), A13 (= A15), N32 (≠ G37), S33 (≠ A38), E34 (= E39), R41 (= R46), P42 (≠ I47), F77 (≠ K82), A78 (≠ I83), S105 (≠ T110), G106 (= G111), L123 (≠ Y130), Y124 (≠ R131), L150 (= L157), G257 (= G277), D258 (≠ E278), G268 (= G288), L269 (= L289), I270 (≠ V290), A273 (≠ L293), K301 (= K322)
4emiA Toluene dioxygenase reductase in reduced state in complex with NAD+ (see paper)
33% identity, 34% coverage: 7:279/810 of query aligns to 4:275/402 of 4emiA
- binding flavin-adenine dinucleotide: G10 (= G13), V11 (≠ M14), G12 (≠ A15), D34 (≠ A38), E35 (= E39), R42 (= R46), P43 (≠ I47), K47 (vs. gap), E78 (≠ K82), V79 (≠ I83), T106 (= T110), G107 (= G111), G273 (= G277), D274 (≠ E278)
- binding nicotinamide-adenine-dinucleotide: R111 (≠ F115), G149 (= G153), L152 (= L156), I153 (≠ L157), E156 (= E160), E172 (≠ H176), A173 (≠ L177), R180 (= R184), V236 (= V240), G237 (= G241), A238 (≠ I242)
Sites not aligning to the query:
P16640 Putidaredoxin reductase CamA; Pdr; Putidaredoxin--NAD(+) reductase; EC 1.18.1.5 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
32% identity, 35% coverage: 1:287/810 of query aligns to 1:293/422 of P16640
- A15 (= A15) binding FAD
- D37 (vs. gap) binding FAD
- K50 (≠ S50) binding FAD
- V83 (≠ I83) binding FAD
- R134 (vs. gap) binding FAD
- D284 (≠ E278) binding FAD
Sites not aligning to the query:
4emjA Complex between the reductase and ferredoxin components of toluene dioxygenase (see paper)
33% identity, 34% coverage: 7:279/810 of query aligns to 5:276/406 of 4emjA
- binding flavin-adenine dinucleotide: G11 (= G13), V12 (≠ M14), G13 (≠ A15), D35 (≠ A38), E36 (= E39), R43 (= R46), P44 (≠ I47), S47 (= S50), K48 (vs. gap), V80 (≠ I83), T107 (= T110), G108 (= G111), R128 (= R131), G274 (= G277), D275 (≠ E278)
Sites not aligning to the query:
1q1wA Crystal structure of putidaredoxin reductase from pseudomonas putida (see paper)
32% identity, 35% coverage: 6:287/810 of query aligns to 5:292/422 of 1q1wA
- active site: L13 (≠ M14), L44 (≠ N45), P45 (≠ R46)
- binding flavin-adenine dinucleotide: G10 (= G11), G12 (= G13), L13 (≠ M14), A14 (= A15), G35 (= G37), D36 (vs. gap), L44 (≠ N45), P45 (≠ R46), K49 (≠ S50), V82 (≠ I83), A108 (= A109), T109 (= T110), G110 (= G111), R133 (vs. gap), I159 (≠ L157), D283 (≠ E278)
Sites not aligning to the query:
6tukB Crystal structure of fdr9 (see paper)
32% identity, 34% coverage: 6:279/810 of query aligns to 3:261/393 of 6tukB
- binding flavin-adenine dinucleotide: V7 (≠ I10), G8 (= G11), G9 (≠ N12), G10 (= G13), A12 (= A15), A34 (= A38), E35 (= E39), R42 (= R46), P43 (≠ I47), K47 (≠ P51), A75 (≠ K82), A76 (≠ I83), T102 (= T110), G103 (= G111), V118 (≠ Y130), R119 (= R131), G259 (= G277), D260 (≠ E278)
Sites not aligning to the query:
3lxdA Crystal structure of ferredoxin reductase arr from novosphingobium aromaticivorans (see paper)
32% identity, 31% coverage: 33:282/810 of query aligns to 31:283/409 of 3lxdA
- active site: R44 (= R46), P45 (≠ I47)
- binding flavin-adenine dinucleotide: R36 (≠ A38), E37 (= E39), R44 (= R46), P45 (≠ I47), S48 (= S50), K49 (≠ P51), E81 (≠ K82), V82 (≠ I83), T109 (= T110), I157 (≠ L157), G278 (= G277), D279 (≠ E278)
Sites not aligning to the query:
Q9LK94 Monodehydroascorbate reductase 4, peroxisomal; AtMDAR4; EC 1.6.5.4 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
31% identity, 29% coverage: 69:303/810 of query aligns to 80:323/488 of Q9LK94
Sites not aligning to the query:
- 11 G→Q: In sdp2-2; loss of ascorbate recycling.
- 14 V→A: In sdp2-1; loss of ascorbate recycling.
- 386 G→Q: In sdp2-3; loss of ascorbate recycling.
- 483:488 mutation Missing: Loss of peroxisomal targeting.
- 488 mutation Missing: No effect on peroxisomal targeting.
Q9HTK9 Rubredoxin-NAD(+) reductase; RdxR; EC 1.18.1.1 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
30% identity, 38% coverage: 1:310/810 of query aligns to 1:315/384 of Q9HTK9
- TGLA 12:15 (≠ NGMA 12:15) binding FAD
- TA 36:37 (≠ GA 37:38) binding FAD
- K45 (≠ R46) binding FAD
- V83 (≠ I83) binding FAD
- E159 (= E160) binding FAD
- D277 (≠ E278) binding FAD
- V289 (= V290) binding FAD
Sites not aligning to the query:
6pfzA Structure of a NAD-dependent persulfide reductase from a. Fulgidus (see paper)
33% identity, 31% coverage: 85:332/810 of query aligns to 88:346/541 of 6pfzA
Sites not aligning to the query:
- active site: 37, 42, 455, 456
- binding calcium ion: 427, 428
- binding coenzyme a: 14, 18, 21, 38, 40, 42, 76, 430, 447, 512, 513, 516
- binding flavin-adenine dinucleotide: 7, 11, 32, 33, 41, 42, 86
3fg2P Crystal structure of ferredoxin reductase for the cyp199a2 system from rhodopseudomonas palustris (see paper)
32% identity, 35% coverage: 7:288/810 of query aligns to 4:285/404 of 3fg2P
- binding flavin-adenine dinucleotide: G8 (= G11), G10 (= G13), H11 (≠ M14), A12 (= A15), D34 (≠ A38), E35 (= E39), R42 (= R46), P43 (≠ I47), S46 (= S50), K47 (≠ P51), R78 (= R81), M79 (≠ I83), T106 (= T110), R127 (= R131), I153 (≠ L157), D275 (≠ E278)
Sites not aligning to the query:
2v3aA Crystal structure of rubredoxin reductase from pseudomonas aeruginosa. (see paper)
30% identity, 38% coverage: 4:310/810 of query aligns to 1:312/381 of 2v3aA
- active site: L11 (≠ M14), K42 (≠ R46), P43 (≠ I47), M290 (≠ F294)
- binding flavin-adenine dinucleotide: I7 (= I10), G8 (= G11), T9 (≠ N12), G10 (= G13), A12 (= A15), T33 (≠ G37), A34 (= A38), D35 (≠ E39), K42 (≠ R46), P43 (≠ I47), R79 (≠ K82), V80 (≠ I83), A105 (= A109), W106 (≠ T110), G107 (= G111), I153 (≠ L157), F157 (≠ A161), D274 (≠ E278), L284 (≠ G288), Y285 (≠ L289), V286 (= V290)
Sites not aligning to the query:
6rvhA Nadh-dependent coenzyme a disulfide reductase soaked with menadione (see paper)
31% identity, 38% coverage: 31:334/810 of query aligns to 28:334/443 of 6rvhA
- active site: V39 (≠ P42), C44 (≠ I47), E53 (= E56), E58 (= E60), Y159 (≠ L156), E163 (= E160)
- binding coenzyme a: S40 (≠ N43), Y41 (= Y44), C44 (≠ I47), R63 (≠ N65), N303 (≠ A301), R307 (= R311)
- binding flavin-adenine dinucleotide: E34 (≠ G37), K35 (≠ A38), A43 (≠ R46), C44 (≠ I47), V81 (≠ I83), T113 (= T110), G114 (= G111), R134 (= R131), Y159 (≠ L156), G280 (= G277), D281 (≠ E278), P297 (= P292), L298 (= L293), G299 (≠ A297)
Sites not aligning to the query:
- active site: 425, 430
- binding coenzyme a: 20, 23, 359, 431, 437, 441, 442
- binding flavin-adenine dinucleotide: 8, 11, 12, 13, 424, 425
- binding menadione: 364, 365, 430, 431
6rvbA Nadh-dependent coenzyme a disulfide reductase soaked with nadh (see paper)
31% identity, 38% coverage: 31:334/810 of query aligns to 28:334/443 of 6rvbA
- active site: V39 (≠ P42), C44 (≠ I47), E53 (= E56), E58 (= E60), Y159 (≠ L156), E163 (= E160)
- binding coenzyme a: S40 (≠ N43), Y41 (= Y44), C44 (≠ I47), R63 (≠ N65), N303 (≠ A301), R307 (= R311)
- binding flavin-adenine dinucleotide: Y33 (≠ F36), E34 (≠ G37), K35 (≠ A38), S36 (≠ E39), A43 (≠ R46), C44 (≠ I47), E80 (≠ K82), V81 (≠ I83), T113 (= T110), G114 (= G111), L133 (≠ Y130), R134 (= R131), I160 (≠ L157), G280 (= G277), D281 (≠ E278), L298 (= L293), G299 (≠ A297)
- binding nicotinamide-adenine-dinucleotide: G156 (= G153), G158 (= G155), Y159 (≠ L156), I160 (≠ L157), E179 (≠ H176), A180 (≠ L177), A240 (= A239), T241 (≠ V240), G242 (= G241), P297 (= P292), I328 (≠ L328), F329 (= F329)
Sites not aligning to the query:
- active site: 425, 430
- binding coenzyme a: 15, 20, 23, 359, 437, 441, 442
- binding flavin-adenine dinucleotide: 8, 11, 12, 13, 424, 425
6ruzA Nadh-dependent coenzyme a disulfide reductase (see paper)
31% identity, 38% coverage: 31:334/810 of query aligns to 28:334/443 of 6ruzA
- active site: V39 (≠ P42), C44 (≠ I47), E53 (= E56), E58 (= E60), Y159 (≠ L156), E163 (= E160)
- binding coenzyme a: S40 (≠ N43), Y41 (= Y44), C44 (≠ I47), R63 (≠ N65), N303 (≠ A301), R307 (= R311)
- binding flavin-adenine dinucleotide: Y33 (≠ F36), E34 (≠ G37), K35 (≠ A38), A43 (≠ R46), C44 (≠ I47), E80 (≠ K82), V81 (≠ I83), T113 (= T110), G114 (= G111), A115 (≠ S112), L133 (≠ Y130), R134 (= R131), Y159 (≠ L156), G280 (= G277), D281 (≠ E278), P297 (= P292), L298 (= L293), G299 (≠ A297)
Sites not aligning to the query:
- active site: 425, 430
- binding coenzyme a: 15, 19, 20, 23, 359, 441, 442
- binding flavin-adenine dinucleotide: 8, 11, 12, 13, 424, 425
8a56B Coenzyme a-persulfide reductase (coapr) from enterococcus faecalis (see paper)
28% identity, 40% coverage: 6:332/810 of query aligns to 2:338/539 of 8a56B
- binding flavin-adenine dinucleotide: I6 (= I10), G7 (= G11), V9 (≠ G13), G11 (≠ A15), E32 (≠ G37), K33 (≠ A38), C42 (≠ I47), V80 (= V93), S109 (≠ A109), P110 (≠ T110), G111 (= G111), I159 (≠ L157), G278 (= G277), D279 (≠ E278), S295 (≠ G288), L296 (= L289), A297 (≠ V290)
- binding 3'-phosphate-adenosine-5'-diphosphate: T17 (≠ E21), R18 (≠ E22), R21 (≠ K25), F70 (≠ I75), R305 (≠ K298)
Sites not aligning to the query:
Query Sequence
>WP_028990131.1 NCBI__GCF_000423825.1:WP_028990131.1
MASKAKLVLIGNGMAGIRTLEELLKMAPGAFEITVFGAEPHPNYNRILLSPVLSGEMRLE
DTILNDWDWYHDHGINLQAGRKIVQIDRARRRVIAADGIEAAYDRLLIATGSNPFLLPIP
GRDLPGVVAYRDISDVEQMLAASAKGGRAVVIGGGLLGLEAANGLLSRGMDVSVVHLHPW
LMERQLDQVGGALLQKALAQRGMRFYLPKQTEAVLGETHVTGLRFRDGEEIPADLVVMAV
GIRPNIELAKAAGIHCERGIVVNDTMQTYDPRIYAVGECVQHRGQTYGLVAPLFEQAKVA
ANHLAAYGRMRYEGSITSTKLKVTGIDLFSAGDFTGGPEVEEITLLDGTYGVYKKVVLKD
NKVVGSVLYGDTSIGPWLFQLLRENHDIGEIRAHLLFGETHLGDAGHAGHDQAASLPDSA
EICGCNGVCKGTIVQAIREQGLFTLEEVRQQTKASSSCGSCTGLVEQLLASTLGGVYDQS
PKKKPLCGCTDLTHDEVREAIVAEKLLSARQVFEYLNWNHPGGCATCRPAVNYYVRTAWP
AEGLDDPQMHFINERAHANIQKDGTYSVVPRIYGGVTSPAELRRIAEVAERFQVPMVKFT
GGQRLDLLGVKKEDLPAIWATLGMPSGHAYGKSLRTVKTCVGTEFCRFGTQDSTRMGIEL
ERALEGMWAPHKVKLAASGCPRNCAESTVKDVGVIGVDSGWEIYIGGNGGMKVRAADLLC
KVKTNAEAIEITKAFLQLYRQEAHYLERTAPWVERVGLEHIKVRVVDDPTNRRRLAEALD
FALAQERDPWAAAIEGHLDVHAEPLARVAP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory