SitesBLAST
Comparing WP_028997153.1 NCBI__GCF_000430725.1:WP_028997153.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
40% identity, 95% coverage: 18:353/353 of query aligns to 22:362/363 of 3dtgA
- active site: K199 (= K195)
- binding O-benzylhydroxylamine: F72 (= F69), Y171 (= Y167), Y204 (= Y200)
- binding pyridoxal-5'-phosphate: R96 (= R93), R189 (= R185), K199 (= K195), Y204 (= Y200), E235 (= E231), G238 (≠ I234), L263 (= L255), I266 (= I258), T267 (= T259), T309 (= T301)
3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
40% identity, 95% coverage: 18:353/353 of query aligns to 22:362/363 of 3dtfA
A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
40% identity, 95% coverage: 18:353/353 of query aligns to 27:367/368 of A0R066
- R101 (= R93) binding pyridoxal 5'-phosphate
- Y209 (= Y200) binding pyridoxal 5'-phosphate
- K299 (≠ R286) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T314 (= T301) binding pyridoxal 5'-phosphate
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
40% identity, 93% coverage: 27:353/353 of query aligns to 3:334/335 of 3ht5A
- active site: K171 (= K195)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R93), K171 (= K195), Y176 (= Y200), E207 (= E231), G210 (≠ I234), M211 (≠ T235), N212 (= N236), L235 (= L255), G237 (= G257), I238 (= I258), T239 (= T259), T281 (= T301)
5i5tA X-ray crystal structure at 2.31a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydroquinoline compound and an internal aldimine linked plp cofactor. (see paper)
37% identity, 98% coverage: 4:350/353 of query aligns to 7:358/361 of 5i5tA
- active site: K198 (= K195)
- binding (3R)-3-methyl-1,2,3,4-tetrahydroquinoline-8-sulfonamide: F26 (= F22), F71 (= F69), Y169 (= Y167), K198 (= K195), T236 (≠ I234), A310 (= A302)
- binding pyridoxal-5'-phosphate: R95 (= R93), R188 (= R185), K198 (= K195), Y203 (= Y200), E233 (= E231), T236 (≠ I234), M237 (≠ T235), N238 (= N236), L262 (= L255), G264 (= G257), V265 (≠ I258), V266 (≠ T259), G308 (= G300), T309 (= T301)
5i5sA X-ray crystal structure at 2.06a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a benzisoxazole compound and an internal aldimine linked plp cofactor. (see paper)
37% identity, 98% coverage: 4:350/353 of query aligns to 7:358/361 of 5i5sA
- active site: K198 (= K195)
- binding 2-(1,2-benzoxazol-3-yl)ethanoic acid: F26 (= F22), F71 (= F69), K198 (= K195), T236 (≠ I234), T309 (= T301), A310 (= A302)
- binding pyridoxal-5'-phosphate: R95 (= R93), R188 (= R185), K198 (= K195), Y203 (= Y200), E233 (= E231), T236 (≠ I234), M237 (≠ T235), N238 (= N236), L262 (= L255), G264 (= G257), V265 (≠ I258), V266 (≠ T259), G308 (= G300), T309 (= T301)
5i5vA X-ray crystal structure at 1.94a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a thienopyrimidine compound and an internal aldimine linked plp cofactor. (see paper)
36% identity, 98% coverage: 4:350/353 of query aligns to 7:357/360 of 5i5vA
- active site: K197 (= K195)
- binding 3,5-dimethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxylic acid: F25 (= F22), K74 (= K73), Y136 (= Y135), Y168 (= Y167), A309 (= A302)
- binding pyridoxal-5'-phosphate: R94 (= R93), R187 (= R185), K197 (= K195), Y202 (= Y200), E232 (= E231), T235 (≠ I234), M236 (≠ T235), L261 (= L255), G263 (= G257), V264 (≠ I258), V265 (≠ T259), G307 (= G300), T308 (= T301)
5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
39% identity, 93% coverage: 27:353/353 of query aligns to 2:325/326 of 5u3fA
- active site: K162 (= K195)
- binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G71), R67 (= R93), Y110 (= Y135), K162 (= K195), Y167 (= Y200), E198 (= E231), G201 (≠ I234), M202 (≠ T235), N203 (= N236), L226 (= L255), G228 (= G257), I229 (= I258), T230 (= T259), T272 (= T301)
5i5yA X-ray crystal structure at 1.81a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with an aryl acetate compound and an internal aldimine linked plp cofactor. (see paper)
36% identity, 98% coverage: 4:350/353 of query aligns to 7:360/363 of 5i5yA
- active site: K200 (= K195)
- binding {2-[(5-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carbonyl)amino]phenyl}acetic acid: F28 (= F22), Y68 (= Y64), F73 (= F69), K77 (= K73), Y139 (= Y135), V153 (= V149), Y171 (= Y167), T238 (≠ I234), T311 (= T301), A312 (= A302)
- binding pyridoxal-5'-phosphate: R97 (= R93), R190 (= R185), K200 (= K195), Y205 (= Y200), E235 (= E231), T238 (≠ I234), L264 (= L255), G266 (= G257), V267 (≠ I258), V268 (≠ T259), T311 (= T301)
5i5uA X-ray crystal structure at 2.40a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydronaphthalenyl compound and an internal aldimine linked plp cofactor. (see paper)
36% identity, 98% coverage: 4:350/353 of query aligns to 7:360/363 of 5i5uA
- active site: K200 (= K195)
- binding 2-hydroxy-N-[(1R)-1,2,3,4-tetrahydronaphthalen-1-yl]acetamide: F28 (= F22), Y68 (= Y64), F73 (= F69), V153 (= V149), K200 (= K195), Y205 (= Y200), T238 (≠ I234), G310 (= G300), T311 (= T301), A312 (= A302)
- binding pyridoxal-5'-phosphate: R97 (= R93), R190 (= R185), K200 (= K195), Y205 (= Y200), E235 (= E231), T238 (≠ I234), M239 (≠ T235), L264 (= L255), G266 (= G257), V267 (≠ I258), V268 (≠ T259), G310 (= G300), T311 (= T301)
O15382 Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT(m); Placental protein 18; PP18; EC 2.6.1.42 from Homo sapiens (Human) (see 6 papers)
36% identity, 98% coverage: 4:350/353 of query aligns to 36:389/392 of O15382
- Y168 (= Y135) binding substrate
- R170 (= R137) to Q: in HVLI; reduced catalytic activity; dbSNP:rs749866079
- T186 (≠ S153) to R: in dbSNP:rs11548193
- K229 (= K195) modified: N6-(pyridoxal phosphate)lysine
- E264 (= E231) to K: in HVLI; reduced catalytic activity; dbSNP:rs767653663
- C342 (≠ T303) mutation to A: Reduces activity about 6-fold.
- C345 (≠ A306) mutation to A: Slight reduction of activity.
2hdkB Crystal structure of cys315ala-cys318ala mutant of human mitochondrial branched chain aminotransferase (see paper)
36% identity, 98% coverage: 4:350/353 of query aligns to 7:360/363 of 2hdkB
- active site: K200 (= K195)
- binding 2-oxo-4-methylpentanoic acid: G310 (= G300), T311 (= T301), A312 (= A302)
- binding pyridoxal-5'-phosphate: R97 (= R93), K200 (= K195), Y205 (= Y200), E235 (= E231), T238 (≠ I234), L264 (= L255), G266 (= G257), V267 (≠ I258), V268 (≠ T259), G310 (= G300), T311 (= T301)
5cr5A X-ray crystal structure at 1.61a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a biphenyl pyrrolidine ether compound and an internal aldimine linked plp cofactor. (see paper)
37% identity, 98% coverage: 4:350/353 of query aligns to 7:355/358 of 5cr5A
- active site: K195 (= K195)
- binding 3-({(3R)-1-[(5-bromothiophen-2-yl)carbonyl]pyrrolidin-3-yl}oxy)-N-methyl-2'-[(methylsulfonyl)amino]biphenyl-4-carboxamide: F23 (= F22), F68 (= F69), Y166 (= Y167), V175 (≠ F175), Q217 (≠ N217), V231 (≠ A232), G232 (= G233), T233 (≠ I234), M234 (≠ T235), G305 (= G300), A307 (= A302), C311 (≠ A306)
- binding pyridoxal-5'-phosphate: R92 (= R93), K195 (= K195), Y200 (= Y200), E230 (= E231), T233 (≠ I234), N235 (= N236), L259 (= L255), G261 (= G257), V262 (≠ I258), V263 (≠ T259), T306 (= T301)
7nwmA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 12 (see paper)
39% identity, 93% coverage: 12:341/353 of query aligns to 18:354/365 of 7nwmA
- binding pyridoxal-5'-phosphate: R98 (= R93), R191 (= R185), K201 (= K195), Y206 (= Y200), E236 (= E231), T239 (≠ I234), M240 (≠ T235), L265 (= L255), G267 (= G257), V268 (≠ I258), T269 (= T259), G311 (= G300), T312 (= T301)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methoxy-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F22), Y69 (= Y64), F74 (= F69), Y140 (= Y135), G153 (= G148), V154 (= V149), Y172 (= Y167), Q213 (≠ L207), Q223 (≠ N217), T239 (≠ I234), A313 (= A302)
7nweA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 10 (see paper)
39% identity, 93% coverage: 12:341/353 of query aligns to 18:354/365 of 7nweA
- binding pyridoxal-5'-phosphate: R98 (= R93), K201 (= K195), Y206 (= Y200), E236 (= E231), T239 (≠ I234), M240 (≠ T235), L265 (= L255), G267 (= G257), V268 (≠ I258), T269 (= T259), G311 (= G300), T312 (= T301)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F22), Y69 (= Y64), F74 (= F69), Y140 (= Y135), G153 (= G148), V154 (= V149), Y172 (= Y167), Q213 (≠ L207), Q223 (≠ N217), T239 (≠ I234), A313 (= A302)
7nwcA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 2 (see paper)
39% identity, 93% coverage: 12:341/353 of query aligns to 18:354/365 of 7nwcA
- binding pyridoxal-5'-phosphate: R98 (= R93), R191 (= R185), K201 (= K195), Y206 (= Y200), E236 (= E231), T239 (≠ I234), M240 (≠ T235), N241 (= N236), L265 (= L255), G267 (= G257), V268 (≠ I258), T269 (= T259), G311 (= G300), T312 (= T301)
- binding 3-(5-chloranyl-2,4-dimethoxy-phenyl)-6-(trifluoromethyl)-1H-pyrimidine-2,4-dione: F28 (= F22), Y172 (= Y167), Q223 (≠ N217), T239 (≠ I234), A313 (= A302)
7nwbA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 1 (see paper)
39% identity, 93% coverage: 12:341/353 of query aligns to 18:354/365 of 7nwbA
- binding pyridoxal-5'-phosphate: R98 (= R93), R191 (= R185), K201 (= K195), Y206 (= Y200), E236 (= E231), T239 (≠ I234), M240 (≠ T235), L265 (= L255), G267 (= G257), V268 (≠ I258), T269 (= T259), G311 (= G300), T312 (= T301)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: F28 (= F22), Y69 (= Y64), F74 (= F69), Y140 (= Y135), G153 (= G148), V154 (= V149), Y172 (= Y167), Q213 (≠ L207), Q223 (≠ N217), A313 (= A302)
7nwaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and compound a (see paper)
39% identity, 93% coverage: 12:341/353 of query aligns to 18:354/365 of 7nwaA
- binding pyridoxal-5'-phosphate: R98 (= R93), K201 (= K195), Y206 (= Y200), E236 (= E231), T239 (≠ I234), N241 (= N236), L265 (= L255), G267 (= G257), V268 (≠ I258), T269 (= T259), T312 (= T301)
- binding 2-[[4-chloranyl-2,6-bis(fluoranyl)phenyl]methylamino]-7-oxidanylidene-5-propyl-4H-pyrazolo[1,5-a]pyrimidine-3-carbonitrile: R142 (= R137), G153 (= G148), V154 (= V149), Y172 (= Y167), F173 (= F168), V181 (≠ F175), Q223 (≠ N217), V237 (≠ A232), T239 (≠ I234), M240 (≠ T235), G311 (= G300), A313 (= A302), C317 (≠ A306)
7nyaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor bay-069 (compound 36) (see paper)
39% identity, 93% coverage: 12:341/353 of query aligns to 17:353/364 of 7nyaA
- binding pyridoxal-5'-phosphate: R97 (= R93), R190 (= R185), K200 (= K195), Y205 (= Y200), E235 (= E231), T238 (≠ I234), M239 (≠ T235), L264 (= L255), G266 (= G257), V267 (≠ I258), T268 (= T259), G310 (= G300), T311 (= T301)
- binding 3-[4-chloranyl-3-(2-methylphenoxy)naphthalen-1-yl]-6-(trifluoromethyl)-5~{H}-pyrimidine-2,4-dione: F27 (= F22), Y68 (= Y64), F73 (= F69), Y139 (= Y135), G152 (= G148), V153 (= V149), Y171 (= Y167), Q212 (≠ L207), Q222 (≠ N217), T238 (≠ I234), A312 (= A302)
7ny9A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 38 (see paper)
39% identity, 93% coverage: 12:341/353 of query aligns to 17:353/364 of 7ny9A
- binding pyridoxal-5'-phosphate: R97 (= R93), R190 (= R185), K200 (= K195), Y205 (= Y200), E235 (= E231), T238 (≠ I234), M239 (≠ T235), N240 (= N236), L264 (= L255), G266 (= G257), V267 (≠ I258), T268 (= T259), T311 (= T301)
- binding 3-[2,6-bis(oxidanylidene)-4-(trifluoromethyl)-5~{H}-pyrimidin-1-yl]-~{N}-methyl-2-pyridin-2-yl-imidazo[1,2-a]pyridine-7-carboxamide: F27 (= F22), Y139 (= Y135), G152 (= G148), V153 (= V149), Y171 (= Y167), Q212 (≠ L207), Q221 (≠ D216), Q222 (≠ N217), V223 (≠ L218), T238 (≠ I234)
Query Sequence
>WP_028997153.1 NCBI__GCF_000430725.1:WP_028997153.1
MEIDVQLRAERKEKLPAAQFGFGEKVSDHMLVMTYDADAGGWNRPEIKPYQSFSLEPRAL
VLHYGQSIFEGLKAFRRPDGHIALFRPEQNIRRMNASARKMAMPEIPEALFMDALLKLVD
IERDWVPAEAGASLYIRPLMFGSDAVLGVRAASRYILCVMLSPVPPYFVPGGKPFKLLAR
EDCARTVAGGTGDAKTAANYGRSLVPLDAARREGYDNLLWLDSEQHRFVEEAGITNVFVQ
YADRVVTPPLSGRILPGITRDSVIRLLRDEGRTVLEQETDIGDLVRDIDDGRVHEIFLTG
TATVVAPVDEIGFRGRRHALQIRDDGLALSLYRQLTQIQYGLAPDRHGWMRAL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory