SitesBLAST
Comparing WP_028998117.1 NCBI__GCF_000430725.1:WP_028998117.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl (see paper)
29% identity, 94% coverage: 15:559/577 of query aligns to 12:561/599 of 1n0hA
- active site: Y31 (≠ V34), G33 (= G36), G34 (≠ E37), A35 (≠ S38), I36 (≠ F39), E57 (= E61), T80 (= T84), F119 (= F123), Q120 (= Q124), E121 (= E125), K169 (≠ E177), R230 (≠ Q232), M266 (≠ I268), V293 (≠ T295), V409 (≠ A399), L434 (≠ A427), G435 (= G428), M437 (= M430), D462 (= D455), N489 (= N487), E491 (≠ T489), Q492 (≠ Y490), M494 (≠ T492), V495 (≠ I493), W498 (≠ H496), L520 (= L518), G525 (= G523), L526 (≠ W524), K559 (≠ A557)
- binding 4-{[(4'-amino-2'-methylpyrimidin-5'-yl)methyl]amino}pent-3-enyl diphosphate: V409 (≠ A399), G410 (= G400), Q411 (≠ N401), H412 (≠ Y402), G435 (= G428), M437 (= M430), G461 (= G454), D462 (= D455), A463 (≠ G456), S464 (≠ D457), M467 (= M460), N489 (= N487), E491 (≠ T489), Q492 (≠ Y490), G493 (= G491), V495 (≠ I493)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: G34 (≠ E37), A35 (≠ S38), V109 (= V113), P110 (≠ A114), F119 (= F123), K169 (≠ E177), M266 (≠ I268), D291 (≠ E293), R292 (≠ M294), V495 (≠ I493), W498 (≠ H496)
- binding flavin-adenine dinucleotide: R159 (= R167), G219 (= G222), A220 (vs. gap), G221 (= G223), N224 (≠ W226), T246 (≠ G248), L247 (≠ F249), Q248 (≠ R250), L264 (≠ V266), G265 (= G267), M266 (≠ I268), H267 (≠ G269), G286 (= G288), A287 (≠ V289), R288 (= R290), D290 (≠ G292), R292 (≠ M294), V293 (≠ T295), E319 (≠ H315), V320 (≠ A316), N324 (≠ E320), G337 (= G339), D338 (≠ K340), A339 (= A341), M414 (≠ G404), G432 (≠ P425), G433 (≠ T426)
- binding magnesium ion: D462 (= D455), N489 (= N487), E491 (≠ T489)
- binding thiamine diphosphate: Y31 (≠ V34), E57 (= E61), P83 (= P87)
5wkcA Saccharomyces cerevisiae acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
29% identity, 94% coverage: 15:559/577 of query aligns to 10:553/591 of 5wkcA
- active site: Y29 (≠ V34), G31 (= G36), G32 (≠ E37), A33 (≠ S38), I34 (≠ F39), E55 (= E61), T78 (= T84), F117 (= F123), Q118 (= Q124), E119 (= E125), K167 (≠ E177), R222 (≠ Q232), M258 (≠ I268), V285 (≠ T295), V401 (≠ A399), L426 (≠ A427), G427 (= G428), M429 (= M430), D454 (= D455), N481 (= N487), E483 (≠ T489), Q484 (≠ Y490), M486 (≠ T492), V487 (≠ I493), W490 (≠ H496), L512 (= L518), G517 (= G523), L518 (≠ W524), K551 (≠ A557)
- binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V401 (≠ A399), G402 (= G400), Q403 (≠ N401), H404 (≠ Y402), G427 (= G428), M429 (= M430), G453 (= G454), D454 (= D455), A455 (≠ G456), S456 (≠ D457), M459 (= M460), N481 (= N487), E483 (≠ T489), Q484 (≠ Y490), G485 (= G491), M486 (≠ T492), V487 (≠ I493)
- binding ethaneperoxoic acid: G32 (≠ E37), Q118 (= Q124)
- binding flavin-adenine dinucleotide: R157 (= R167), G211 (= G222), A212 (vs. gap), G213 (= G223), N216 (≠ W226), T238 (≠ G248), L239 (≠ F249), Q240 (≠ R250), L256 (≠ V266), M258 (≠ I268), G278 (= G288), A279 (≠ V289), R280 (= R290), R284 (≠ M294), V285 (≠ T295), E311 (≠ H315), V312 (≠ A316), N316 (≠ E320), D330 (≠ K340), A331 (= A341), M406 (≠ G404), G424 (≠ P425)
- binding magnesium ion: D454 (= D455), N481 (= N487), E483 (≠ T489)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: G32 (≠ E37), A33 (≠ S38), V107 (= V113), F117 (= F123), K167 (≠ E177), M258 (≠ I268), R284 (≠ M294), M486 (≠ T492), W490 (≠ H496)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: P30 (= P35), E55 (= E61)
5dx6B Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
32% identity, 94% coverage: 15:556/577 of query aligns to 19:540/557 of 5dx6B
- active site: I38 (≠ V34), G40 (= G36), A41 (≠ E37), K42 (≠ S38), I43 (≠ F39), E63 (= E61), T86 (= T84), H125 (≠ F123), Q126 (= Q124), S127 (≠ E125), Q175 (≠ E177), L268 (≠ I268), E295 (≠ T295), M392 (≠ A399), Q418 (≠ G428), M420 (= M430), D445 (= D455), D472 (= D486), G474 (= G488), Y475 (= Y490), M477 (≠ T492), V478 (≠ I493), Q481 (≠ H496)
- binding 3-fluoro-2-oxopropanoic acid: G264 (= G264), R265 (≠ D265), Q272 (≠ P272), A400 (≠ H407), R401 (= R408), Y404 (vs. gap)
- binding magnesium ion: S135 (≠ G133), T138 (= T136), D445 (= D455), D472 (= D486), G474 (= G488)
- binding thiamine diphosphate: G393 (= G400), S394 (≠ N401), F395 (≠ Y402), Q418 (≠ G428), M420 (= M430), G444 (= G454), D445 (= D455), G446 (= G456), D472 (= D486), G474 (= G488), Y475 (= Y490), N476 (≠ G491), M477 (≠ T492), V478 (≠ I493)
Sites not aligning to the query:
1t9bB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
28% identity, 94% coverage: 15:559/577 of query aligns to 10:557/595 of 1t9bB
- active site: Y29 (≠ V34), G31 (= G36), G32 (≠ E37), A33 (≠ S38), I34 (≠ F39), E55 (= E61), T78 (= T84), F117 (= F123), Q118 (= Q124), E119 (= E125), K167 (≠ E177), R226 (≠ Q232), M262 (≠ I268), V289 (≠ T295), V405 (≠ A399), L430 (≠ A427), G431 (= G428), M433 (= M430), D458 (= D455), N485 (= N487), E487 (≠ T489), Q488 (≠ Y490), M490 (≠ T492), V491 (≠ I493), W494 (≠ H496), L516 (= L518), G521 (= G523), L522 (≠ W524), K555 (≠ A557)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (= V113), P108 (≠ A114), D287 (≠ E293), R288 (≠ M294), M490 (≠ T492), W494 (≠ H496)
- binding flavin-adenine dinucleotide: R157 (= R167), G215 (= G222), A216 (vs. gap), G217 (= G223), N220 (≠ W226), T242 (≠ G248), L243 (≠ F249), Q244 (≠ R250), M259 (≠ D265), L260 (≠ V266), M262 (≠ I268), H263 (≠ G269), G282 (= G288), A283 (≠ V289), R284 (= R290), D286 (≠ G292), R288 (≠ M294), V289 (≠ T295), E315 (≠ H315), V316 (≠ A316), N320 (≠ E320), G333 (= G339), D334 (≠ K340), A335 (= A341), Q409 (≠ S403), M410 (≠ G404), G428 (≠ P425), G429 (≠ T426)
- binding magnesium ion: D458 (= D455), N485 (= N487), E487 (≠ T489)
1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
29% identity, 94% coverage: 15:559/577 of query aligns to 10:558/596 of 1t9cA
- active site: Y29 (≠ V34), G31 (= G36), G32 (≠ E37), A33 (≠ S38), I34 (≠ F39), E55 (= E61), T78 (= T84), F117 (= F123), Q118 (= Q124), E119 (= E125), K167 (≠ E177), R227 (≠ Q232), M263 (≠ I268), V290 (≠ T295), V406 (≠ A399), L431 (≠ A427), G432 (= G428), M434 (= M430), D459 (= D455), N486 (= N487), E488 (≠ T489), Q489 (≠ Y490), M491 (≠ T492), V492 (≠ I493), W495 (≠ H496), L517 (= L518), G522 (= G523), L523 (≠ W524), K556 (≠ A557)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ E37), V107 (= V113), P108 (≠ A114), F117 (= F123), K167 (≠ E177), D288 (≠ E293), R289 (≠ M294), W495 (≠ H496)
- binding flavin-adenine dinucleotide: R157 (= R167), G216 (= G222), A217 (vs. gap), G218 (= G223), N221 (≠ W226), T243 (≠ G248), L244 (≠ F249), Q245 (≠ R250), L261 (≠ V266), M263 (≠ I268), H264 (≠ G269), G283 (= G288), A284 (≠ V289), R285 (= R290), D287 (≠ G292), R289 (≠ M294), V290 (≠ T295), E316 (≠ H315), V317 (≠ A316), N321 (≠ E320), G334 (= G339), D335 (≠ K340), A336 (= A341), M411 (≠ G404), G429 (≠ P425), G430 (≠ T426)
- binding magnesium ion: D459 (= D455), N486 (= N487), E488 (≠ T489)
1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
29% identity, 94% coverage: 15:559/577 of query aligns to 10:558/596 of 1t9dA
- active site: Y29 (≠ V34), G31 (= G36), G32 (≠ E37), A33 (≠ S38), I34 (≠ F39), E55 (= E61), T78 (= T84), F117 (= F123), Q118 (= Q124), E119 (= E125), K167 (≠ E177), R227 (≠ Q232), M263 (≠ I268), V290 (≠ T295), V406 (≠ A399), L431 (≠ A427), G432 (= G428), M434 (= M430), D459 (= D455), N486 (= N487), E488 (≠ T489), Q489 (≠ Y490), M491 (≠ T492), V492 (≠ I493), W495 (≠ H496), L517 (= L518), G522 (= G523), L523 (≠ W524), K556 (≠ A557)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (≠ E37), A33 (≠ S38), V107 (= V113), P108 (≠ A114), F117 (= F123), K167 (≠ E177), M263 (≠ I268), D288 (≠ E293), R289 (≠ M294), W495 (≠ H496)
- binding flavin-adenine dinucleotide: R157 (= R167), G216 (= G222), A217 (vs. gap), G218 (= G223), N221 (≠ W226), T243 (≠ G248), L244 (≠ F249), Q245 (≠ R250), M260 (≠ D265), L261 (≠ V266), H264 (≠ G269), G283 (= G288), A284 (≠ V289), R285 (= R290), D287 (≠ G292), R289 (≠ M294), V290 (≠ T295), E316 (≠ H315), V317 (≠ A316), N321 (≠ E320), G334 (= G339), D335 (≠ K340), A336 (= A341), Q410 (≠ S403), M411 (≠ G404), G429 (≠ P425), G430 (≠ T426)
- binding magnesium ion: D459 (= D455), N486 (= N487), E488 (≠ T489)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E61), P81 (= P87), Q118 (= Q124), G432 (= G428), M434 (= M430), M464 (= M460)
6u9dB Saccharomyces cerevisiae acetohydroxyacid synthase (see paper)
28% identity, 94% coverage: 15:559/577 of query aligns to 14:569/607 of 6u9dB
- active site: Y33 (≠ V34), G35 (= G36), G36 (≠ E37), A37 (≠ S38), I38 (≠ F39), E59 (= E61), T82 (= T84), F121 (= F123), Q122 (= Q124), E123 (= E125), K171 (≠ E177), M274 (≠ I268), V301 (≠ T295), V417 (≠ A399), G443 (= G428), M445 (= M430), D470 (= D455), N497 (= N487), E499 (≠ T489), Q500 (≠ Y490), M502 (≠ T492), V503 (≠ I493), W506 (≠ H496)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: G36 (≠ E37), V111 (= V113), P112 (≠ A114), F121 (= F123), K171 (≠ E177), D299 (≠ E293), R300 (≠ M294), M502 (≠ T492), W506 (≠ H496)
- binding flavin-adenine dinucleotide: R161 (= R167), A228 (vs. gap), G229 (= G223), N232 (≠ W226), T254 (≠ G248), L255 (≠ F249), Q256 (≠ R250), L272 (≠ V266), M274 (≠ I268), G294 (= G288), R296 (= R290), D298 (≠ G292), R300 (≠ M294), V301 (≠ T295), E327 (≠ H315), V328 (≠ A316), N332 (≠ E320), D346 (≠ K340), A347 (= A341), M422 (≠ G404), G440 (≠ P425), G441 (≠ T426)
- binding magnesium ion: D470 (= D455), N497 (= N487)
- binding thiamine diphosphate: E59 (= E61), P85 (= P87), V417 (≠ A399), G418 (= G400), Q419 (≠ N401), H420 (≠ Y402), G443 (= G428), M445 (= M430), A471 (≠ G456), S472 (≠ D457), N497 (= N487), E499 (≠ T489), Q500 (≠ Y490), G501 (= G491), M502 (≠ T492), V503 (≠ I493)
1t9aA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
29% identity, 94% coverage: 15:559/577 of query aligns to 11:559/597 of 1t9aA
- active site: Y30 (≠ V34), G32 (= G36), G33 (≠ E37), A34 (≠ S38), I35 (≠ F39), E56 (= E61), T79 (= T84), F118 (= F123), Q119 (= Q124), E120 (= E125), K168 (≠ E177), R228 (≠ Q232), M264 (≠ I268), V291 (≠ T295), V407 (≠ A399), L432 (≠ A427), G433 (= G428), M435 (= M430), D460 (= D455), N487 (= N487), E489 (≠ T489), Q490 (≠ Y490), M492 (≠ T492), V493 (≠ I493), W496 (≠ H496), L518 (= L518), G523 (= G523), L524 (≠ W524), K557 (≠ A557)
- binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: G33 (≠ E37), V108 (= V113), P109 (≠ A114), F118 (= F123), K168 (≠ E177), M264 (≠ I268), D289 (≠ E293), R290 (≠ M294), M492 (≠ T492), V493 (≠ I493), W496 (≠ H496)
- binding flavin-adenine dinucleotide: R158 (= R167), G217 (= G222), A218 (vs. gap), G219 (= G223), N222 (≠ W226), T244 (≠ G248), L245 (≠ F249), Q246 (≠ R250), L262 (≠ V266), M264 (≠ I268), H265 (≠ G269), G284 (= G288), A285 (≠ V289), R286 (= R290), D288 (≠ G292), R290 (≠ M294), V291 (≠ T295), E317 (≠ H315), V318 (≠ A316), N322 (≠ E320), G335 (= G339), D336 (≠ K340), A337 (= A341), Q411 (≠ S403), M412 (≠ G404), G430 (≠ P425), G431 (≠ T426)
- binding magnesium ion: D460 (= D455), N487 (= N487), E489 (≠ T489)
- binding propyl trihydrogen diphosphate: V407 (≠ A399), G408 (= G400), Q409 (≠ N401), H410 (≠ Y402), M435 (= M430), G459 (= G454), D460 (= D455), A461 (≠ G456), S462 (≠ D457), N487 (= N487), E489 (≠ T489), Q490 (≠ Y490), G491 (= G491), M492 (≠ T492)
- binding 5-{[ethyl(methyl)amino]methyl}-2-methyl-5,6-dihydropyrimidin-4-amine: G433 (= G428), M435 (= M430), M465 (= M460)
6derA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide metosulam (see paper)
26% identity, 94% coverage: 15:559/577 of query aligns to 16:562/600 of 6derA
- active site: Y35 (≠ V34), G37 (= G36), G38 (≠ E37), A39 (≠ S38), I40 (≠ F39), E61 (= E61), T84 (= T84), F123 (= F123), Q124 (= Q124), E125 (= E125), K173 (≠ E177), K231 (≠ Q232), M267 (≠ I268), V294 (≠ T295), V410 (≠ A399), L435 (vs. gap), G436 (= G428), M438 (= M430), D463 (= D455), N490 (= N487), E492 (≠ T489), Q493 (≠ Y490), M495 (≠ T492), V496 (≠ I493), W499 (≠ H496), L521 (= L518), N526 (≠ G523), V527 (≠ W524)
- binding flavin-adenine dinucleotide: R163 (= R167), G220 (= G222), A221 (≠ G223), G222 (≠ S224), N225 (≠ S227), T247 (≠ G248), L248 (≠ F249), Q249 (≠ R250), L265 (≠ V266), H268 (≠ G269), G287 (= G288), A288 (≠ V289), R289 (= R290), D291 (≠ G292), R293 (≠ M294), V294 (≠ T295), E320 (≠ H315), I321 (≠ A316), N325 (≠ E320), G338 (≠ C333), D339 (≠ S334), V340 (≠ L335), Q414 (≠ S403), M415 (≠ G404), G433 (≠ T426)
- binding Metosulam: R293 (≠ M294), M495 (≠ T492), W499 (≠ H496)
- binding magnesium ion: D463 (= D455), N490 (= N487), E492 (≠ T489)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V410 (≠ A399), G411 (= G400), Q412 (≠ N401), H413 (≠ Y402), G436 (= G428), M438 (= M430), G462 (= G454), D463 (= D455), A464 (≠ G456), S465 (≠ D457), N490 (= N487), E492 (≠ T489), Q493 (≠ Y490), G494 (= G491), M495 (≠ T492), V496 (≠ I493)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V410 (≠ A399), G411 (= G400), Q412 (≠ N401), H413 (≠ Y402), G436 (= G428), M438 (= M430), G462 (= G454), D463 (= D455), A464 (≠ G456), S465 (≠ D457), M468 (= M460), N490 (= N487), E492 (≠ T489), Q493 (≠ Y490), G494 (= G491), V496 (≠ I493)
Sites not aligning to the query:
6desA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide propoxycarbazone (see paper)
26% identity, 94% coverage: 15:559/577 of query aligns to 14:560/598 of 6desA
- active site: Y33 (≠ V34), G35 (= G36), G36 (≠ E37), A37 (≠ S38), I38 (≠ F39), E59 (= E61), T82 (= T84), F121 (= F123), Q122 (= Q124), E123 (= E125), K171 (≠ E177), K229 (≠ Q232), M265 (≠ I268), V292 (≠ T295), V408 (≠ A399), L433 (vs. gap), G434 (= G428), M436 (= M430), D461 (= D455), N488 (= N487), E490 (≠ T489), Q491 (≠ Y490), M493 (≠ T492), V494 (≠ I493), W497 (≠ H496), L519 (= L518), N524 (≠ G523), V525 (≠ W524)
- binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: M265 (≠ I268), D290 (≠ E293), R291 (≠ M294), W497 (≠ H496)
- binding flavin-adenine dinucleotide: R161 (= R167), G218 (= G222), A219 (≠ G223), G220 (≠ S224), N223 (≠ S227), T245 (≠ G248), L246 (≠ F249), Q247 (≠ R250), L263 (≠ V266), G285 (= G288), A286 (≠ V289), R287 (= R290), D289 (≠ G292), R291 (≠ M294), V292 (≠ T295), E318 (≠ H315), I319 (≠ A316), N323 (≠ E320), D337 (≠ S334), V338 (≠ L335), Q412 (≠ S403), M413 (≠ G404), G431 (≠ T426)
- binding magnesium ion: D461 (= D455), N488 (= N487), E490 (≠ T489)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (≠ A399), G409 (= G400), Q410 (≠ N401), H411 (≠ Y402), G434 (= G428), M436 (= M430), G460 (= G454), D461 (= D455), A462 (≠ G456), S463 (≠ D457), N488 (= N487), E490 (≠ T489), Q491 (≠ Y490), G492 (= G491), M493 (≠ T492), V494 (≠ I493)
6depA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide sulfometuron methyl (see paper)
26% identity, 94% coverage: 15:559/577 of query aligns to 14:560/598 of 6depA
- active site: Y33 (≠ V34), G35 (= G36), G36 (≠ E37), A37 (≠ S38), I38 (≠ F39), E59 (= E61), T82 (= T84), F121 (= F123), Q122 (= Q124), E123 (= E125), K171 (≠ E177), K229 (≠ Q232), M265 (≠ I268), V292 (≠ T295), V408 (≠ A399), L433 (vs. gap), G434 (= G428), M436 (= M430), D461 (= D455), N488 (= N487), E490 (≠ T489), Q491 (≠ Y490), M493 (≠ T492), V494 (≠ I493), W497 (≠ H496), L519 (= L518), N524 (≠ G523), V525 (≠ W524)
- binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D290 (≠ E293), R291 (≠ M294), M493 (≠ T492), W497 (≠ H496)
- binding flavin-adenine dinucleotide: R161 (= R167), G218 (= G222), A219 (≠ G223), G220 (≠ S224), N223 (≠ S227), T245 (≠ G248), L246 (≠ F249), Q247 (≠ R250), L263 (≠ V266), G264 (= G267), G285 (= G288), A286 (≠ V289), R287 (= R290), D289 (≠ G292), R291 (≠ M294), V292 (≠ T295), E318 (≠ H315), I319 (≠ A316), N323 (≠ E320), D337 (≠ S334), V338 (≠ L335), M413 (≠ G404), G431 (≠ T426)
- binding magnesium ion: D461 (= D455), N488 (= N487), E490 (≠ T489)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V408 (≠ A399), G409 (= G400), Q410 (≠ N401), H411 (≠ Y402), G434 (= G428), M436 (= M430), G460 (= G454), D461 (= D455), A462 (≠ G456), S463 (≠ D457), M466 (= M460), N488 (= N487), E490 (≠ T489), Q491 (≠ Y490), G492 (= G491), M493 (≠ T492), V494 (≠ I493)
- binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V408 (≠ A399), G409 (= G400), Q410 (≠ N401), H411 (≠ Y402), G434 (= G428), M436 (= M430), G460 (= G454), D461 (= D455), A462 (≠ G456), S463 (≠ D457), M466 (= M460), N488 (= N487), E490 (≠ T489), Q491 (≠ Y490), G492 (= G491), M493 (≠ T492), V494 (≠ I493)
P07342 Acetolactate synthase catalytic subunit, mitochondrial; Acetohydroxy-acid synthase catalytic subunit; AHAS; ALS; EC 2.2.1.6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 94% coverage: 15:559/577 of query aligns to 94:649/687 of P07342
- R241 (= R167) binding FAD
- 355:376 (vs. 269:290, 41% identical) binding FAD
- 407:426 (vs. 315:340, 15% identical) binding FAD
6denA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron ethyl (see paper)
27% identity, 94% coverage: 15:559/577 of query aligns to 16:561/599 of 6denA
- active site: Y35 (≠ V34), G37 (= G36), G38 (≠ E37), A39 (≠ S38), I40 (≠ F39), E61 (= E61), T84 (= T84), F123 (= F123), Q124 (= Q124), E125 (= E125), K173 (≠ E177), K230 (≠ Q232), M266 (≠ I268), V293 (≠ T295), V409 (≠ A399), L434 (vs. gap), G435 (= G428), M437 (= M430), D462 (= D455), N489 (= N487), E491 (≠ T489), Q492 (≠ Y490), M494 (≠ T492), V495 (≠ I493), W498 (≠ H496), L520 (= L518), N525 (≠ G523), V526 (≠ W524)
- binding flavin-adenine dinucleotide: R163 (= R167), G219 (= G222), A220 (≠ G223), G221 (≠ S224), N224 (≠ S227), T246 (≠ G248), L247 (≠ F249), Q248 (≠ R250), L264 (≠ V266), G286 (= G288), A287 (≠ V289), R288 (= R290), D290 (≠ G292), R292 (≠ M294), V293 (≠ T295), E319 (≠ H315), I320 (≠ A316), N324 (≠ E320), D338 (≠ S334), V339 (≠ L335), M414 (≠ G404), G432 (≠ T426)
- binding ethyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M266 (≠ I268), D291 (≠ E293), R292 (≠ M294), W498 (≠ H496)
- binding magnesium ion: D462 (= D455), N489 (= N487), E491 (≠ T489)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V409 (≠ A399), G410 (= G400), Q411 (≠ N401), H412 (≠ Y402), G435 (= G428), M437 (= M430), G461 (= G454), D462 (= D455), A463 (≠ G456), S464 (≠ D457), N489 (= N487), E491 (≠ T489), Q492 (≠ Y490), G493 (= G491), M494 (≠ T492), V495 (≠ I493)
1t9dB Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
28% identity, 94% coverage: 15:559/577 of query aligns to 9:544/582 of 1t9dB
- active site: Y28 (≠ V34), G30 (= G36), G31 (≠ E37), A32 (≠ S38), I33 (≠ F39), E54 (= E61), T77 (= T84), F116 (= F123), Q117 (= Q124), E118 (= E125), K166 (≠ E177), R213 (≠ Q232), M249 (≠ I268), V276 (≠ T295), V392 (≠ A399), L417 (≠ A427), G418 (= G428), M420 (= M430), D445 (= D455), N472 (= N487), E474 (≠ T489), Q475 (≠ Y490), M477 (≠ T492), V478 (≠ I493), W481 (≠ H496), L503 (= L518), G508 (= G523), L509 (≠ W524), K542 (≠ A557)
- binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G31 (≠ E37), A32 (≠ S38), V106 (= V113), P107 (≠ A114), F116 (= F123), K166 (≠ E177), M249 (≠ I268), D274 (≠ E293), R275 (≠ M294), W481 (≠ H496)
- binding flavin-adenine dinucleotide: R156 (= R167), G202 (= G222), A203 (vs. gap), G204 (= G223), N207 (≠ W226), T229 (≠ G248), L230 (≠ F249), Q231 (≠ R250), L247 (≠ V266), M249 (≠ I268), H250 (≠ G269), G269 (= G288), A270 (≠ V289), R271 (= R290), D273 (≠ G292), R275 (≠ M294), V276 (≠ T295), E302 (≠ H315), V303 (≠ A316), N307 (≠ E320), G320 (= G339), D321 (≠ K340), A322 (= A341), Q396 (≠ S403), M397 (≠ G404), G415 (≠ P425), G416 (≠ T426)
- binding magnesium ion: D445 (= D455), N472 (= N487), E474 (≠ T489)
- binding 2,5-dimethyl-pyrimidin-4-ylamine: E54 (= E61), P80 (= P87), G418 (= G428), M420 (= M430), M450 (= M460)
1t9bA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
28% identity, 94% coverage: 15:559/577 of query aligns to 10:545/583 of 1t9bA
- active site: Y29 (≠ V34), G31 (= G36), G32 (≠ E37), A33 (≠ S38), I34 (≠ F39), E55 (= E61), T78 (= T84), F117 (= F123), Q118 (= Q124), E119 (= E125), K167 (≠ E177), R214 (≠ Q232), M250 (≠ I268), V277 (≠ T295), V393 (≠ A399), L418 (≠ A427), G419 (= G428), M421 (= M430), D446 (= D455), N473 (= N487), E475 (≠ T489), Q476 (≠ Y490), M478 (≠ T492), V479 (≠ I493), W482 (≠ H496), L504 (= L518), G509 (= G523), L510 (≠ W524), K543 (≠ A557)
- binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: V107 (= V113), P108 (≠ A114), F117 (= F123), D275 (≠ E293), R276 (≠ M294), M478 (≠ T492), W482 (≠ H496)
- binding flavin-adenine dinucleotide: R157 (= R167), G203 (= G222), A204 (vs. gap), G205 (= G223), N208 (≠ W226), T230 (≠ G248), L231 (≠ F249), Q232 (≠ R250), M247 (≠ D265), L248 (≠ V266), M250 (≠ I268), H251 (≠ G269), G270 (= G288), A271 (≠ V289), R272 (= R290), D274 (≠ G292), R276 (≠ M294), V277 (≠ T295), E303 (≠ H315), V304 (≠ A316), N308 (≠ E320), D322 (≠ K340), A323 (= A341), Q397 (≠ S403), M398 (≠ G404), G416 (≠ P425), G417 (≠ T426)
- binding magnesium ion: D446 (= D455), N473 (= N487), E475 (≠ T489)
6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
26% identity, 94% coverage: 15:559/577 of query aligns to 14:559/597 of 6demA
- active site: Y33 (≠ V34), G35 (= G36), G36 (≠ E37), A37 (≠ S38), I38 (≠ F39), E59 (= E61), T82 (= T84), F121 (= F123), Q122 (= Q124), E123 (= E125), K171 (≠ E177), K228 (≠ Q232), M264 (≠ I268), V291 (≠ T295), V407 (≠ A399), L432 (vs. gap), G433 (= G428), M435 (= M430), D460 (= D455), N487 (= N487), E489 (≠ T489), Q490 (≠ Y490), M492 (≠ T492), V493 (≠ I493), W496 (≠ H496), L518 (= L518), N523 (≠ G523), V524 (≠ W524)
- binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (≠ I268), D289 (≠ E293), R290 (≠ M294), M492 (≠ T492), W496 (≠ H496)
- binding flavin-adenine dinucleotide: R161 (= R167), G217 (= G222), A218 (≠ G223), G219 (≠ S224), N222 (≠ S227), T244 (≠ G248), L245 (≠ F249), Q246 (≠ R250), L262 (≠ V266), G284 (= G288), A285 (≠ V289), R286 (= R290), D288 (≠ G292), R290 (≠ M294), V291 (≠ T295), E317 (≠ H315), I318 (≠ A316), N322 (≠ E320), D336 (≠ S334), V337 (≠ L335), M412 (≠ G404), G430 (≠ T426)
- binding magnesium ion: D460 (= D455), N487 (= N487), E489 (≠ T489)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A399), G408 (= G400), Q409 (≠ N401), H410 (≠ Y402), M435 (= M430), G459 (= G454), D460 (= D455), A461 (≠ G456), S462 (≠ D457), M465 (= M460), N487 (= N487), E489 (≠ T489), Q490 (≠ Y490), G491 (= G491), M492 (≠ T492), V493 (≠ I493)
Sites not aligning to the query:
6delA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide chlorimuron ethyl (see paper)
26% identity, 94% coverage: 15:559/577 of query aligns to 14:559/597 of 6delA
- active site: Y33 (≠ V34), G35 (= G36), G36 (≠ E37), A37 (≠ S38), I38 (≠ F39), E59 (= E61), T82 (= T84), F121 (= F123), Q122 (= Q124), E123 (= E125), K171 (≠ E177), K228 (≠ Q232), M264 (≠ I268), V291 (≠ T295), V407 (≠ A399), L432 (vs. gap), G433 (= G428), M435 (= M430), D460 (= D455), N487 (= N487), E489 (≠ T489), Q490 (≠ Y490), M492 (≠ T492), V493 (≠ I493), W496 (≠ H496), L518 (= L518), N523 (≠ G523), V524 (≠ W524)
- binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: D289 (≠ E293), R290 (≠ M294), W496 (≠ H496)
- binding flavin-adenine dinucleotide: R161 (= R167), G217 (= G222), A218 (≠ G223), G219 (≠ S224), N222 (≠ S227), T244 (≠ G248), L245 (≠ F249), Q246 (≠ R250), L262 (≠ V266), G284 (= G288), A285 (≠ V289), R286 (= R290), D288 (≠ G292), R290 (≠ M294), V291 (≠ T295), E317 (≠ H315), I318 (≠ A316), N322 (≠ E320), D336 (≠ S334), V337 (≠ L335), M412 (≠ G404), G430 (≠ T426)
- binding (3Z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl](formyl)amino}-3-sulfanylpent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A399), G408 (= G400), Q409 (≠ N401), H410 (≠ Y402), G433 (= G428), M435 (= M430), G459 (= G454), D460 (= D455), A461 (≠ G456), S462 (≠ D457), M465 (= M460), N487 (= N487), E489 (≠ T489), Q490 (≠ Y490), G491 (= G491), M492 (≠ T492), V493 (≠ I493)
- binding magnesium ion: D460 (= D455), N487 (= N487), E489 (≠ T489)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (≠ A399), G408 (= G400), Q409 (≠ N401), H410 (≠ Y402), G433 (= G428), M435 (= M430), G459 (= G454), D460 (= D455), A461 (≠ G456), S462 (≠ D457), M465 (= M460), N487 (= N487), E489 (≠ T489), Q490 (≠ Y490), G491 (= G491), M492 (≠ T492), V493 (≠ I493)
6deqA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
26% identity, 94% coverage: 15:559/577 of query aligns to 16:563/601 of 6deqA
- active site: Y35 (≠ V34), G37 (= G36), G38 (≠ E37), A39 (≠ S38), I40 (≠ F39), E61 (= E61), T84 (= T84), F123 (= F123), Q124 (= Q124), E125 (= E125), K173 (≠ E177), K232 (≠ Q232), M268 (≠ I268), V295 (≠ T295), V411 (≠ A399), L436 (vs. gap), G437 (= G428), M439 (= M430), D464 (= D455), N491 (= N487), E493 (≠ T489), Q494 (≠ Y490), M496 (≠ T492), V497 (≠ I493), W500 (≠ H496), L522 (= L518), N527 (≠ G523), V528 (≠ W524)
- binding flavin-adenine dinucleotide: R163 (= R167), G221 (= G222), A222 (≠ G223), G223 (≠ S224), N226 (≠ S227), T248 (≠ G248), L249 (≠ F249), Q250 (≠ R250), L266 (≠ V266), G288 (= G288), A289 (≠ V289), R290 (= R290), D292 (≠ G292), R294 (≠ M294), V295 (≠ T295), E321 (≠ H315), I322 (≠ A316), D340 (≠ S334), V341 (≠ L335), M416 (≠ G404), G434 (≠ T426)
- binding magnesium ion: D464 (= D455), N491 (= N487), E493 (≠ T489)
- binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M268 (≠ I268), R294 (≠ M294), M496 (≠ T492), V497 (≠ I493), W500 (≠ H496)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V411 (≠ A399), G412 (= G400), Q413 (≠ N401), H414 (≠ Y402), M439 (= M430), G463 (= G454), D464 (= D455), A465 (≠ G456), S466 (≠ D457), N491 (= N487), E493 (≠ T489), Q494 (≠ Y490), G495 (= G491), M496 (≠ T492), V497 (≠ I493)
Sites not aligning to the query:
6deoA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide iodomuron methyl (see paper)
26% identity, 94% coverage: 15:559/577 of query aligns to 12:555/593 of 6deoA
- active site: Y31 (≠ V34), G33 (= G36), G34 (≠ E37), A35 (≠ S38), I36 (≠ F39), E57 (= E61), T80 (= T84), F119 (= F123), Q120 (= Q124), E121 (= E125), K169 (≠ E177), K224 (≠ Q232), M260 (≠ I268), V287 (≠ T295), V403 (≠ A399), L428 (vs. gap), G429 (= G428), M431 (= M430), D456 (= D455), N483 (= N487), E485 (≠ T489), Q486 (≠ Y490), M488 (≠ T492), V489 (≠ I493), W492 (≠ H496), L514 (= L518), N519 (≠ G523), V520 (≠ W524)
- binding flavin-adenine dinucleotide: R159 (= R167), G213 (= G222), A214 (≠ G223), G215 (≠ S224), N218 (≠ S227), T240 (≠ G248), L241 (≠ F249), Q242 (≠ R250), L258 (≠ V266), G280 (= G288), A281 (≠ V289), R282 (= R290), D284 (≠ G292), R286 (≠ M294), V287 (≠ T295), E313 (≠ H315), I314 (≠ A316), N318 (≠ E320), D332 (≠ S334), V333 (≠ L335), M408 (≠ G404), G426 (≠ T426)
- binding methyl 2-{[(4-iodo-6-methoxypyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: M260 (≠ I268), D285 (≠ E293), R286 (≠ M294), M488 (≠ T492), W492 (≠ H496)
- binding magnesium ion: D456 (= D455), N483 (= N487), E485 (≠ T489)
- binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V403 (≠ A399), G404 (= G400), Q405 (≠ N401), H406 (≠ Y402), G429 (= G428), M431 (= M430), G455 (= G454), D456 (= D455), A457 (≠ G456), S458 (≠ D457), M461 (= M460), N483 (= N487), E485 (≠ T489), Q486 (≠ Y490), G487 (= G491), M488 (≠ T492), V489 (≠ I493)
6dekA Crystal structure of candida albicans acetohydroxyacid synthase catalytic subunit (see paper)
26% identity, 94% coverage: 15:559/577 of query aligns to 15:557/595 of 6dekA
- active site: Y34 (≠ V34), G36 (= G36), G37 (≠ E37), A38 (≠ S38), I39 (≠ F39), E60 (= E61), T83 (= T84), Q118 (= Q124), E119 (= E125), K167 (≠ E177), K226 (≠ Q232), M262 (≠ I268), V289 (≠ T295), V405 (≠ A399), L430 (vs. gap), G431 (= G428), M433 (= M430), D458 (= D455), N485 (= N487), E487 (≠ T489), Q488 (≠ Y490), M490 (≠ T492), V491 (≠ I493), W494 (≠ H496), L516 (= L518), N521 (≠ G523), V522 (≠ W524)
- binding flavin-adenine dinucleotide: R157 (= R167), G215 (= G222), A216 (≠ G223), G217 (≠ S224), N220 (≠ S227), T242 (≠ G248), L243 (≠ F249), Q244 (≠ R250), L260 (≠ V266), M262 (≠ I268), G282 (= G288), A283 (≠ V289), R284 (= R290), D286 (≠ G292), R288 (≠ M294), V289 (≠ T295), E315 (≠ H315), I316 (≠ A316), N320 (≠ E320), D334 (≠ S334), V335 (≠ L335), M410 (≠ G404), G428 (≠ T426)
- binding magnesium ion: D458 (= D455), N485 (= N487), E487 (≠ T489)
- binding thiamine diphosphate: V405 (≠ A399), G406 (= G400), Q407 (≠ N401), H408 (≠ Y402), M433 (= M430), G457 (= G454), D458 (= D455), A459 (≠ G456), S460 (≠ D457), N485 (= N487), E487 (≠ T489), Q488 (≠ Y490), G489 (= G491), M490 (≠ T492), V491 (≠ I493)
Query Sequence
>WP_028998117.1 NCBI__GCF_000430725.1:WP_028998117.1
MSGGREEAVQNTRPGGHLLVEALIAQGVDTVFGVPGESFLAALDGMHERRDAIRFIACRH
EGGAAFMAEAQGKLTGRPGVCFVTRGPGATNAAIGVHTAFQDSTPLLLLVGQVARHQRDR
EAFQELDFRQVFGPGTLGMAKWVAEVDDAERLPEYVARAFHVAMQGRPGPVVLALPEDVL
SGPAAAPVLPRVEPAQAWPAPGALRQLRTLLLQAERPLVIAGGSGWSAESAQALQRFAEN
WNLPVGCGFRFQDTFDNRHPNYAGDVGIGINPRLAQRVRDADLLIAVGVRLGEMTTGGYT
LIEPPRPKQKLVHLHAGAEELGRVYAADLLLQCSLAAAGKALETLTAPPQLPWAGWTASA
RADFEANRVPPPVAPLDMAQVVLAIERLAPADSLYTNGAGNYSGWLHRYLRYRGLQHHGR
TQLAPTAGAMGYGVPAAVAAALLYPQRTAINLAGDGDFLMTGQELATAVAYGANRGAGRL
VSIVVDNGTYGTIRMHQERHYPGRVSGSDLFNPDFAALARSYGWRAARVDTTEAFEPAFQ
EALSEGPPMLLHLKLDAEVSTSRSTLSQIREAALKRP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory