SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_028999445.1 NCBI__GCF_000430725.1:WP_028999445.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

6sulA Amicoumacin kinase amin in complex with amp-pnp, mg2+ and ami (see paper)
24% identity, 51% coverage: 68:232/326 of query aligns to 71:236/334 of 6sulA

query
sites
6sulA

M

L

R

H

H

H

L

L

A

A

Q

K

R

G

G

G

I

L

P

S

V

V

P

A

E

K

P

P

R

I

S

A

D

S

D

L

N

N

D

G

Q

R

I

D

L

I

H

E

Q

Q

L

V

K

D

D

D

K

G

P

Q

A

G

A

G

-

S

-

F

-

L

-

L

-

R

L

V

V

Y

N

E

K
|
K

L
x
A

P

P

G

G

G

H

H
x
K

Q

V

L

E

A

E

P

A

D

D

L

W

H

N

H

D

-

E

-

L

C

F

E

Y

Q

A

V

L

G

G

E

Q

M

Y

L

T

A

G

R

R

M

M

H

H

L

K

A

L

G

T

Q

K

D

S

F

Y

P

Q

L

L

A

S

Q

D

P

P

N

R

L

Y

R

K

G

R

L

Q

P

E

W

W

W

D

Q

E

E
|
E

T

E

V
x
Q

P

L

V

K

V

L

L

R

P

K

F

Y

L

V

T

P

A

A

E

D

Q

Q

R

T

T

L

L

V

I

F

E

E

E

Q

E

A

L

D

A

R

F

L

Q

M

T

E

A

K

L

L

A

A

E

K

S

L

A

P

A

K

Y

N

L

Q

D

D

L

-

P

T

R

Y

G

G

P

L

V

V

H

H

A

A

D
|
D

L

L

F
x
H

R
x
H

D
x
G

N
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N

V

F

M

H

F

W

E

D

G

-

L

-

P

-

G

-

R

Q

E

G

R

K

L

I

S

T

G

T

F

-

F
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F

D
|
D

F

F

Y

D

F
x
D

A

I

G

G

V

Y

D

N

T

W

F

F

L

M

F

N

D

D

I

I

A

S

V

I

C

L

L

L

binding phosphoaminophosphonic acid-adenylate ester: K111 (= K103), A112 (≠ L104), K116 (≠ H108), H204 (≠ F195), G206 (≠ D197), N207 (= N198), F218 (= F214), D219 (= D215)
binding magnesium ion: N207 (= N198), D219 (= D215)
binding Amicoumacin A: E159 (= E149), Q161 (≠ V151), D202 (= D193), H204 (≠ F195), H205 (≠ R196), G206 (≠ D197), D222 (≠ F218)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 32, 36, 37, 39, 50, 52
binding Amicoumacin A: 36, 238, 242, 290, 293

Query Sequence

>WP_028999445.1 NCBI__GCF_000430725.1:WP_028999445.1
MAVFTEVTFDEASAFVATLDVGQVTALRGIGAGIENTNYFVSTTQGEWVLTLFERLTFEQ
LPFYLHLMRHLAQRGIPVPEPRSDDNDQILHQLKDKPAALVNKLPGGHQLAPDLHHCEQV
GEMLARMHLAGQDFPLAQPNLRGLPWWQETVPVVLPFLTAEQRTLIEEELAFQTALAESA
AYLDLPRGPVHADLFRDNVMFEGLPGRERLSGFFDFYFAGVDTFLFDIAVCLNDWCIDLD
SGRGVDERAAVFVAAYERVRPLTGNERRLLPALLRAGALRFWVSRLWDLHLPRDASMLKP
HDPEHFERVLRARIAVPWHPQGEREA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory