SitesBLAST
Comparing WP_029001265.1 NCBI__GCF_000430725.1:WP_029001265.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 12 hits to proteins with known functional sites (download)
6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
28% identity, 83% coverage: 35:407/448 of query aligns to 16:385/409 of 6e9nA
P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
27% identity, 83% coverage: 35:407/448 of query aligns to 27:404/430 of P0AA76
- Y29 (= Y37) binding D-galactonate
- D31 (= D39) mutation to N: Loss of galactonate transport activity.
- R32 (= R40) binding D-galactonate
- Y64 (= Y72) binding D-galactonate
- E118 (= E126) mutation to Q: Loss of galactonate transport activity.
- W358 (= W361) binding D-galactonate
6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
28% identity, 83% coverage: 35:407/448 of query aligns to 19:369/393 of 6e9oA
P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
22% identity, 67% coverage: 1:301/448 of query aligns to 64:402/534 of P53322
- K283 (≠ R217) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
8u3gA Structure of naag-bound sialin (see paper)
22% identity, 80% coverage: 33:392/448 of query aligns to 15:380/427 of 8u3gA
8u3hA Structure of fmoc-leu-oh bound sialin (see paper)
22% identity, 80% coverage: 33:392/448 of query aligns to 15:378/425 of 8u3hA
Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
22% identity, 73% coverage: 66:392/448 of query aligns to 113:441/495 of Q9NRA2
- K136 (≠ R94) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
- H183 (≠ M134) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
- LL 198:199 (≠ VV 149:150) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- IL 266:267 (≠ LE 223:224) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
- SSL------RN 268:272 (≠ SRAVIDQAGHA 225:235) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
- G328 (= G288) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
- P334 (≠ V294) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
- G371 (= G320) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794
Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
23% identity, 78% coverage: 43:392/448 of query aligns to 90:441/495 of Q5Q0U0
- K136 (≠ R89) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
- R168 (= R119) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- E171 (≠ L122) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
- G172 (= G123) mutation to C: Decreases protein levels and alters subcellular localization.
- E175 (= E126) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
- G176 (≠ A127) mutation to C: Decreases protein levels and alters subcellular localization.
- F179 (= F130) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
- P180 (= P131) mutation to C: Decreases protein levels and alters subcellular localization.
- H183 (≠ M134) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- W186 (≠ V137) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
- S-SLKN 268:272 (≠ SRAVID 225:230) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
- P334 (≠ V294) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
- G371 (= G320) mutation to V: Remains in the endoplasmic reticulum.
Sites not aligning to the query:
- 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
- 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
22% identity, 78% coverage: 43:392/448 of query aligns to 90:441/495 of Q8BN82
- H183 (≠ M134) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.
Sites not aligning to the query:
- 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.
Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
22% identity, 54% coverage: 50:289/448 of query aligns to 128:369/570 of Q9C0U9
Sites not aligning to the query:
- 14 modified: Phosphoserine
Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
24% identity, 41% coverage: 68:250/448 of query aligns to 90:272/493 of Q03567
Sites not aligning to the query:
- 69 modified: carbohydrate, N-linked (GlcNAc...) asparagine
Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
24% identity, 36% coverage: 26:185/448 of query aligns to 117:271/605 of Q9GQQ0
- E217 (= E126) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.
Query Sequence
>WP_029001265.1 NCBI__GCF_000430725.1:WP_029001265.1
METRSIESGVDETHRVATQKAMTRLIPLLCAIYFMSYIDRTNVSLAKAHLAADLGISAAA
YGLGAGIFFLGYALLEVPSNLLAHRIGPRPWIARIAVTWGALSVAMMFVQGEASFYLLRV
LLGIAEAGLFPALMYMVTLWFAPKDRPVVVGWIYTAPALGLLLGNPLGGALMQLDGLAGL
RGWQWMFMLEGLPTIAVGILLYFKLAERPSDAKWLSREEAQALESRAVIDQAGHANLSSQ
DWMAAIKRPSTVLTGLIYFLNQVAFVGLVFFTPAMIQQMKVESPFLVGVMSSSVGLGFLL
GVLTLPRIHRRVKSDFVFLGALTAGLLLSACAFLATERPAVQIALFVATAFFGGGILPSY
WAIAMKRLQGIQAAAGLAFINTIGLLGGFVGPYLFGMVETATGRSSAGFSVILGAAALGL
LLVPALAKASRSERQAPELADPVIKPVS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory