SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_029001753.1 NCBI__GCF_000430725.1:WP_029001753.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
35% identity, 99% coverage: 3:452/455 of query aligns to 17:469/472 of P78061

query
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P78061

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H282 (= H265) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R341) mutation to Q: Activity is impaired to 3% of wild-type.

8oozA Glutamine synthetase (see paper)
33% identity, 84% coverage: 70:452/455 of query aligns to 46:427/430 of 8oozA

query
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8oozA

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binding adenosine-5'-triphosphate: G117 (≠ A142), E170 (≠ D204), F185 (= F219), K186 (≠ F220), Y187 (≠ H221), N233 (≠ H267), S235 (= S269), S315 (≠ A349), R317 (= R351)
binding magnesium ion: E119 (= E144), H231 (= H265), E319 (= E353)

8ooxB Glutamine synthetase (see paper)
33% identity, 82% coverage: 80:452/455 of query aligns to 62:435/438 of 8ooxB

query
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8ooxB

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binding magnesium ion: E129 (= E146), E183 (= E209), E190 (= E216)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
30% identity, 98% coverage: 7:452/455 of query aligns to 4:441/444 of P12425

query
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P12425

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R

V

Q

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

E

N

I

K

V

V

V

M

A

F

M

D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

G

-

P

-

Y

-

D
x
G

V

F

I

V

T
x
R

P

I

T

E

D

E

R

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

A

N

T

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

R

P

D

D

G

G

R

T

L

P

V

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

C

L

D

K

L

E

Y

M

A

E

A

D

A

L

G

G

L

F

E

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

G

D

E

P

P

D

T

M

L

P

E

L

L

K

N

P

D

P

K

I

G

G

G

R

Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

I
x
V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

A

H

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

K

H

H

D

G

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

L

S

S

V

L

L

F

R

K

K

-

D

-

T

N

G

G

L

V

N

N

I

A

F

F

-

F

-

D

S

E

N

N

A

A

D

D

G

L

S

Q

P

L

S

S

R

E

E

T

F

A

Y

K

W

H

Y

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

V

A

P

T

A

S

A

F

M

T

A

A

L

V

F

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

A

V

R

P

F
x
G

T

Y

A
x
E

A

A

P
|
P

I

C

N

Y

I

V

Q

A

W

W

G

S

T

A

D

Q

N

N

R

R

T

S

V

P

G

L

I

I

R

R

S

I

P

P

V

A

A

S

P

R

P

G

A

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

V

S

V

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

S

A

V

T

L

L

L

A

A

C

A

G

G

W

L

L

D

G

G

I

I

K

K

N

N

R

K

I

L

E

E

P

A

T

P

P

A

E

P

C

I

K

D

G

R

D

N

A

I

Y

Y

L

V

-

M

-

S

-

K

-

E

-

R

-

M

-

E

-

N

G

G

D

I

Y

V

A

D

L

L

P

P

R

A

S

T

L

L

G

A

E

E

A

A

L

L

E

E

K

E

L

F

R

K

A

S

E

N

E

E

A

V

L

M

A

V

E

K

V

A

L

L

G

G

E

E

E

H

F

L

V

F

T

E

V

H

Y

F

T

I

E

E

V

A

K

K

E

E

T

I

E
|
E

F

W

A

D

E

M

F

F

M

R

K

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

G59 (≠ D74) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ T77) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E144) binding Mg(2+)
E134 (= E146) binding Mg(2+)
E189 (= E209) binding Mg(2+)
V190 (≠ I210) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E216) binding Mg(2+)
G241 (= G261) binding L-glutamate
H245 (= H265) binding Mg(2+)
G302 (≠ F322) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ A324) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P326) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E353) binding Mg(2+)
E424 (= E435) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
30% identity, 98% coverage: 7:452/455 of query aligns to 3:440/443 of 4lnkA

query
sites
4lnkA

F

Y

T

T

Y

R

S

E

D

D

L

I

E

E

H

K

W

L

L

V

D

K

Q

E

H

E

R

N

V

V

T

K

E

Y

I

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

Q

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M
x
K

R

A

L

L

P

D

E

N

I

K

V

V

V
x
M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

G

-

P

-

Y

-

D

G

V

F

I

V

T

R

P

I

T

E

D

E

R

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

A

N

T

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

R

P

D

D

G

G

R

T

L

P

V

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

C

L

D

K

L

E

Y

M

A

E

A

D

A

L

G

G

L

F

E

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

G

D

E

P

P

D

T

M

L

P

E

L

L

K

N

P

D

P

K

I

G

G

G

R

Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

I
x
V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F
|
F

F

K

H
x
Y

A

A

H

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

K

H

H

D

G

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H
x
N

Q

L

S
|
S

V

L

L

F

R

K

K

-

D

-

T

N

G

G

L

V

N

N

I

A

F

F

-

F

-

D

S

E

N

N

A

A

D

D

G

L

S

Q

P

L

S

S

R

E

E

T

F

A

Y

K

W

H

Y

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

V

A

P

T

A

S

A

F

M

T

A

A

L

V

F

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

A

V

R

P

F

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

Q

A

W

W

G

S

T

A

D

Q

N

N

R
|
R

T

S

V

P

G

L

I

I

R

R

S

I

P

P

V

A

A
x
S

P

R

P

G

A

I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

S

V

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

S

A

V

T

L

L

L

A

A

C

A

G

G

W

L

L

D

G

G

I

I

K

K

N

N

R

K

I

L

E

E

P

A

T

P

P

A

E

P

C

I

K

D

G

R

D

N

A

I

Y

Y

L

V

-

M

-

S

-

K

-

E

-

R

-

M

-

E

-

N

G

G

D

I

Y

V

A

D

L

L

P

P

R

A

S

T

L

L

G

A

E

E

A

A

L

L

E

E

K

E

L

F

R

K

A

S

E

N

E

E

A

V

L

M

A

V

E

K

V

A

L

L

G

G

E

E

E

H

F

L

V

F

T

E

V

H

Y

F

T

I

E

E

V

A

K

K

E

E

T

I

E

E

F

W

A

D

E

M

F

F

M

R

K

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ M60), E131 (= E144), E133 (= E146), E188 (= E209), E195 (= E216), H244 (= H265), R315 (= R336), E332 (= E353), R334 (= R355)
binding adenosine-5'-diphosphate: K43 (≠ M51), M50 (≠ V58), F198 (= F219), Y200 (≠ H221), N246 (≠ H267), S248 (= S269), S324 (≠ A345), S328 (≠ A349), R330 (= R351)
binding glutamic acid: E133 (= E146), E188 (= E209), V189 (≠ I210), N239 (≠ P260), G240 (= G261), G242 (≠ A263), E303 (≠ A324)
binding magnesium ion: E131 (= E144), E188 (= E209), E195 (= E216), H244 (= H265), E332 (= E353)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
30% identity, 98% coverage: 7:452/455 of query aligns to 3:440/443 of 4lniA

query
sites
4lniA

F

Y

T

T

Y

R

S

E

D

D

L

I

E

E

H

K

W

L

L

V

D

K

Q

E

H

E

R

N

V

V

T

K

E

Y

I

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

Q

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

E

N

I

K

V

V

V

M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

G

-

P

-

Y

-

D

G

V

F

I

V

T

R

P

I

T

E

D

E

R

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

A

N

T

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

R

P

D

D

G

G

R

T

L

P

V

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

C

L

D

K

L

E

Y

M

A

E

A

D

A

L

G

G

L

F

E

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

G

D

E

P

P

D

T

M

L

P

E

L

L

K

N

P

D

P

K

I

G

G

G

R

Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

F

K

H
x
Y

A

A

H

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

K

H

H

D

G

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

I

C

H
x
N

Q

L

S
|
S

V

L

L

F

R

K

K

-

D

-

T

N

G

G

L

V

N

N

I

A

F

F

-

F

-

D

S

E

N

N

A

A

D

D

G

L

S

Q

P

L

S

S

R

E

E

T

F

A

Y

K

W

H

Y

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

V

A

P

T

A

S

A

F

M

T

A

A

L

V

F

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

A

V

R

P

F

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

Q

A

W

W

G

S

T

A

D

Q

N

N

R
|
R

T

S

V

P

G

L

I

I

R
|
R

S

I

P

P

V

A

A

S

P

R

P

G

A

I

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

S

V

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

S

A

V

T

L

L

L

A

A

C

A

G

G

W

L

L

D

G

G

I

I

K

K

N

N

R

K

I

L

E

E

P

A

T

P

P

A

E

P

C

I

K

D

G

R

D

N

A

I

Y

Y

L

V

-

M

-

S

-

K

-

E

-

R

-

M

-

E

-

N

G

G

D

I

Y

V

A

D

L

L

P

P

R

A

S

T

L

L

G

A

E

E

A

A

L

L

E

E

K

E

L

F

R

K

A

S

E

N

E

E

A

V

L

M

A

V

E

K

V

A

L

L

G

G

E

E

E

H

F

L

V

F

T

E

V

H

Y

F

T

I

E

E

V

A

K

K

E

E

T

I

E

E

F

W

A

D

E

M

F

F

M

R

K

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ M60), E131 (= E144), E133 (= E146), E188 (= E209), E195 (= E216), H244 (= H265), R315 (= R336), E332 (= E353), R334 (= R355)
binding adenosine-5'-diphosphate: E131 (= E144), E183 (≠ D204), D197 (≠ N218), Y200 (≠ H221), N246 (≠ H267), S248 (= S269), R320 (= R341), R330 (= R351)
binding magnesium ion: E131 (= E144), E131 (= E144), E133 (= E146), E188 (= E209), E195 (= E216), E195 (= E216), H244 (= H265), E332 (= E353)
binding l-methionine-s-sulfoximine phosphate: E133 (= E146), E188 (= E209), H244 (= H265), R297 (= R318), E303 (≠ A324), R315 (= R336), R334 (= R355)

4s0rD Structure of gs-tnra complex (see paper)
30% identity, 98% coverage: 7:452/455 of query aligns to 7:444/447 of 4s0rD

query
sites
4s0rD

F

Y

T

T

Y

R

S

E

D

D

L

I

E

E

H

K

W

L

L

V

D

K

Q

E

H

E

R

N

V

V

T

K

E

Y

I

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

T

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

Q

S

K

Q

F

L

T

-

E

-

D

-

R

-

G

G

M

K

R

A

L

L

P

D

E

N

I

K

V

V

V

M

A

F

M
x
D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

G

-

P

-

Y

-

D

G

V
x
F

I
x
V

T
x
R

P
x
I

T

E

D

E

R

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

A

N

T

T

V

F

R

V

I

I

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

H

C

D

D

C

I

F

Y

D

N

R

P

D

D

G

G

R

T

L

P

V

F

P

E

F

G

A

D

P

P

R

R

S

N

V

N

L

L

R

K

R

R

V

I

C

L

D

K

L

E

Y

M

A

E

A

D

A

L

G

G

L

F

E

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

G

D

E

P

P

D

T

M

L

P

E

L

L

K

N

P

D

P

K

I

G

G

G

R

Y

S

F

G
x
D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

A

H

G

P

A

L

V

G

R

L

S

A

C

D

D

E

D

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

K

H

H

D

G

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G
|
G

E
x
V

P
x
N

G

G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

L

S

S

V

L

L

F

R

K

K

-

D

-

T

N

G

G

L

V

N

N

I

A

F

F

-

F

-

D

S

E

N

N

A

A

D

D

G

L

S

Q

P

L

S

S

R

E

E

T

F

A

Y

K

W

H

Y

F

I

I

G

A

G

G

L

I

Q

V

K

K

Y

H

V

A

P

T

A

S

A

F

M

T

A

A

L

V

F

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

A

V

R

P

F
x
G

T
x
Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

Q

A

W

W

G

S

T

A

D

Q

N

N

R
|
R

T

S

V

P

G

L

I

I

R

R

S

I

P

P

V

A

A

S

P

R

P

G

A

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R
|
R

V

S

V

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

S

A

V

T

L

L

L

A

A

C

A

G

G

W

L

L

D

G

G

I

I

K

K

N

N

R

K

I

L

E

E

P

A

T

P

P

A

E

P

C

I

K

D

G

R

D

N

A

I

Y
|
Y

L

V

-

M

-

S

-

K

-

E

-

R

-

M

-

E

-

N

G

G

D

I

Y

V

A

D

L

L

P

P

R

A

S

T

L

L

G

A

E

E

A

A

L

L

E

E

K

E

L

F

R

K

A

S

E

N

E

E

A

V

L

M

A

V

E

K

V

A

L

L

G

G

E

E

E

H

F

L

V

F

T

E

V
x
H

Y

F

T

I

E

E

V

A

K

K

E

E

T
x
I

E

E

F

W

A

D

E
x
M

F

F

M

R

K

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D56 (≠ M60), E135 (= E144), E137 (= E146), E192 (= E209), E199 (= E216), H248 (= H265), R319 (= R336), E336 (= E353), R338 (= R355)
binding glutamine: E137 (= E146), E192 (= E209), R301 (= R318), E307 (≠ A324)
binding magnesium ion: I66 (≠ P78), E135 (= E144), E135 (= E144), E199 (= E216), H248 (= H265), H248 (= H265), E336 (= E353), H419 (≠ V427)
binding : F63 (≠ V75), V64 (≠ I76), R65 (≠ T77), I66 (≠ P78), D161 (≠ G168), G241 (= G258), V242 (≠ E259), N243 (≠ P260), G305 (≠ F322), Y306 (≠ T323), Y376 (= Y393), I426 (≠ T434), M430 (≠ E438)

8s59O Glutamine synthetase
33% identity, 82% coverage: 80:452/455 of query aligns to 68:443/446 of 8s59O

query
sites
8s59O

D

E

R

S

D

D

M

M

H

K

L

L

R

A

P

L

D

D

P

P

A

S

T

T

V

F

R

R

I

I

V

L

P

P

W

W

-
x
R

-

P

A

A

T

T

D

G

P

A

T
x
V

A

A

Q

R

V

I

I

L

H

G

D

D

C

V

F

Y

D

L

R

P

D

D

G

G

R

N

L

P

V

F

P

K

F

G

A

D

P

P

R

R

S

Y

V

V

L

L

R

K

R

T

V

A

C

I

D

K

L

E

Y

A

A

E

A

K

A

M

G

G

L

F

E

S

P

M

V

N

V

V

A

G

P

P

E

E

L

L

E

E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

A

N

N

T

G

D

N

P

P

D

T

M

T

P

E

L

L

K

T

P

D

P

Q

I

G

G

G

R

Y

S

F

G

D

R

F

A

A

E

P

T

L

S

D

R

R

Q

A

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D
x
Q

P

D

L

V

F
x
R

E
x
R

D

D

V

I

Y

D

D

Y

Y

A

C

L

E

E

K

H

M

M

E

G

L

F

N

Q

V

I

D

E

T

A

L
x
S

I

H

H

H

E

E

I

V

G

A

A

P

G

S

Q

Q

M

H

E

E

I

I

N

D

F

F

F

R

H

F

A

G

H

D

P

V

L

L

G

C

L

T

A

A

D

D

E

N

V

V

F

V

F

T

F

F

K

K

R

Y

T

V

V

V

R

K

E

S

A

I

A

A

M

Y

R

H

H

K

D

G

M

Y

F

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H

H

I

S

H

N

Q

Q

S

S

V

L

L

F

R

K

K

D

D

-

T

-

G

G

L

K

N

N

I

V

F

F

S

Y

N

D

A

P

D

D

G

T

-

P

-

T

S

K

P

L

S

S

R

Q

E

D

F

A

Y

M

W

Y

Y

Y

I

I

G

G

L

L

Q

L

K

K

Y

H

V

I

P

R

A

E

A

F

M

T

A

A

L

V

F

T

A

N

P

P

Y

V

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

A

V

R

P

F

G

T

Y

A

E

A

A

P

P

I

V

N

Y

I

I

Q

S

W

W

G

S

T

A

D

Q

N

N

R

R

T

S

V

S

G

L

I

I

R

R

S

I

P

P

V

A

A

T

P

R

P

G

A

N

A

G

R

T

R

R

V

I

E

E

N

L

R

R

V

C

V

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

A

A

L

T

M

L

L

A

R

C

A

G

G

W

L

L

E

G

G

I

I

K

K

N

N

R

K

I

I

E

D

P

P

-

G

T

E

P

P

E

T

C

N

K

V

G

N

D

I

A

F

Y

H

L

L

G

S

D

D

Y

K

-

E

-

R

-

E

-

R

-

G

-

I

-

R

A

S

L

L

P

P

R

A

S

D

L

L

G

K

E

E

A

A

L

I

E

D

K

E

L

M

R

K

A

G

E

S

E

K

A

F

L

V

A

K

E

E

V

A

L

L

G

G

E

E

E

H

F

V

V

F

T

S

V

H

Y

Y

T

L

E

C

V

A

K

K

E

E

T

M

E

E

F

W

A

D

E

E

F

Y

M

K

K

A

V

V

I

V

S

H

P

P

W

W

E

E

R

L

E

S

H

R

L

Y

L

L

binding 2-oxoglutaric acid: R86 (vs. gap), V92 (≠ T102), Q168 (≠ D186), R171 (≠ F189), R172 (≠ E190), S188 (≠ L206)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 23, 25, 39

8tfbA Glutamine synthetase (see paper)
33% identity, 82% coverage: 80:452/455 of query aligns to 65:440/443 of 8tfbA

query
sites
8tfbA

D

E

R

S

D

D

M

M

H

K

L

L

R

A

P

L

D

D

P

P

A

S

T

T

V

F

R

R

I

I

V

L

P

P

W

W

-

R

-

P

A

A

T

T

D

G

P

A

T

V

A

A

Q

R

V

I

I

L

H

G

D

D

C

V

F

Y

D

L

R

P

D

D

G

G

R

N

L

P

V

F

P

K

F

G

A

D

P

P

R

R

S

Y

V

V

L

L

R

K

R

T

V

A

C

I

D

K

L

E

Y

A

A

E

A

K

A

M

G

G

L

F

E

S

P

M

V

N

V

V

A

G

P

P

E

E

L

L

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

A

N

N

T

G

D

N

P

P

D

T

M

T

P

E

L

L

K

T

P

D

P

Q

I

G

G

G

R

Y

S

F

G

D

R

F

A

A

E

P

T

L

S

D

R

R

Q

A

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

Q

P

D

L

V

F

R

E

R

D

D

V

I

Y

D

D

Y

Y

A

C

L

E

E

K

H

M

M

E

G

L

F

N

Q

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

S

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

R

H

F

A

G

H

D

P

V

L

L

G

C

L

T

A

A

D

D

E

N

V

V

F

V

F

T

F

F

K

K

R

Y

T

V

V

V

R

K

E

S

A

I

A

A

M

Y

R

H

H

K

D

G

M

Y

F

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H

H

I

S

H

N

Q

Q

S

S

V

L

L

F

R

K

K

D

D

-

T

-

G

G

L

K

N

N

I

V

F

F

S

Y

N

D

A

P

D

D

G

T

S

P

P

T

-

K

-

L

S

S

R

Q

E

D

F

A

Y

M

W

Y

Y

Y

I

I

G

G

L

L

Q

L

K

K

Y

H

V

I

P

R

A

E

A

F

M

T

A

A

L

V

F

T

A

N

P

P

Y

V

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

A

V

R

P

F

G

T

Y

A

E

A

A

P

P

I

V

N

Y

I

I

Q

S

W

W

G

S

T

A

D

Q

N

N

R

R

T

S

V

S

G

L

I

I

R

R

S

I

P

P

V

A

A

T

P

R

P

G

A

N

A

G

R

T

R

R

V

I

E

E

N

L

R

R

V

C

V

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

A

A

L

T

M

L

L

A

R

C

A

G

G

W

L

L

E

G

G

I

I

K

K

N

N

R

K

I

I

E

D

P

P

-

G

T

E

P

P

E

T

C

N

K

V

G

N

D

I

A

F

Y

H

L

L

G

S

D

D

Y

K

-

E

-

R

-

E

-

R

-

G

-

I

-

R

A

S

L

L

P

P

R

A

S

D

L

L

G

K

E

E

A

A

L

I

E

D

K

E

L

M

R

K

A

G

E

S

E

K

A

F

L

V

A

K

E

E

V

A

L

L

G

G

E

E

E

H

F

V

V

F

T

S

V

H

Y

Y

T

L

E

C

V

A

K

K

E

E

T

M

E

E

F

W

A

D

E

E

F

Y

M

K

K

A

V

V

I

V

S

H

P

P

W

W

E

E

R

L

E

S

H

R

L

Y

L

L

binding magnesium ion: E133 (= E146), E188 (= E209), E195 (= E216)

8tfkA Glutamine synthetase (see paper)
33% identity, 82% coverage: 80:452/455 of query aligns to 62:437/440 of 8tfkA

query
sites
8tfkA

D

E

R

S

D

D

M

M

H

K

L

L

R

A

P

L

D

D

P

P

A

S

T

T

V

F

R

R

I

I

V

L

P

P

W

W

-

R

-

P

A

A

T

T

D

G

P

A

T

V

A

A

Q

R

V

I

I

L

H

G

D

D

C

V

F

Y

D

L

R

P

D

D

G

G

R

N

L

P

V

F

P

K

F

G

A

D

P

P

R

R

S

Y

V

V

L

L

R

K

R

T

V

A

C

I

D

K

L

E

Y

A

A

E

A

K

A

M

G

G

L

F

E

S

P

M

V

N

V

V

A

G

P

P

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

A

N

N

T

G

D

N

P

P

D

T

M

T

P

E

L

L

K

T

P

D

P

Q

I

G

G

G

R

Y

S

F

G

D

R

F

A

A

E

P

T

L

S

D

R

R

Q

A

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

Q

P

D

L

V

F

R

E

R

D

D

V

I

Y

D

D

Y

Y

A

C

L

E

E

K

H

M

M

E

G

L

F

N

Q

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

S

Q

Q

M

H

E
|
E

I

I

N
x
D

F
|
F

F

R

H
x
F

A

G

H

D

P

V

L

L

G

C

L

T

A

A

D

D

E

N

V

V

F

V

F

T

F

F

K

K

R

Y

T

V

V

V

R

K

E

S

A

I

A

A

M

Y

R

H

H

K

D

G

M

Y

F

Y

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

S

H
x
N

Q

Q

S

S

V

L

L

F

R

K

K

D

D

-

T

-

G

G

L

K

N

N

I

V

F

F

S

Y

N

D

A

P

D

D

G

T

S

P

P

T

-

K

-

L

S

S

R

Q

E

D

F

A

Y

M

W

Y

Y

Y

I

I

G

G

L

L

Q

L

K

K

Y

H

V

I

P

R

A

E

A

F

M

T

A

A

L

V

F

T

A

N

P

P

Y

V

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

A

V

R

P

F

G

T

Y

A
x
E

A

A

P

P

I

V

N

Y

I

I

Q

S

W

W

G

S

T

A

D

Q

N

N

R
|
R

T

S

V

S

G

L

I

I

R
|
R

S

I

P

P

V

A

A

T

P

R

P

G

A

N

A
x
G

R

T

R
|
R

V

I

E
|
E

N

L

R
|
R

V

C

V

P

G

D

A

P

D

A

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

A

A

L

T

M

L

L

A

R

C

A

G

G

W

L

L

E

G

G

I

I

K

K

N

N

R

K

I

I

E

D

P

P

-

G

T

E

P

P

E

T

C

N

K

V

G

N

D

I

A

F

Y

H

L

L

G

S

D

D

Y

K

-

E

-

R

-

E

-

R

-

G

-

I

-

R

A

S

L

L

P

P

R

A

S

D

L

L

G

K

E

E

A

A

L

I

E

D

K

E

L

M

R

K

A

G

E

S

E

K

A

F

L

V

A

K

E

E

V

A

L

L

G

G

E

E

E

H

F

V

V

F

T

S

V

H

Y

Y

T

L

E

C

V

A

K

K

E

E

T

M

E

E

F

W

A

D

E

E

F

Y

M

K

K

A

V

V

I

V

S

H

P

P

W

W

E

E

R

L

E

S

H

R

L

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E144), D194 (≠ N218), F195 (= F219), F197 (≠ H221), N243 (≠ H267), R312 (= R336), R317 (= R341), G325 (≠ A349), R327 (= R351)
binding magnesium ion: E128 (= E144), E128 (= E144), E130 (= E146), E185 (= E209), E192 (= E216), E192 (= E216), H241 (= H265), E329 (= E353)
binding l-methionine-s-sulfoximine phosphate: E128 (= E144), E130 (= E146), E185 (= E209), E192 (= E216), G237 (= G261), H241 (= H265), R294 (= R318), E300 (≠ A324), R312 (= R336), R331 (= R355)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
31% identity, 84% coverage: 68:447/455 of query aligns to 51:440/447 of 8oooA

query
sites
8oooA

E

E

E

N

G

G

P

L

Y

Y

Y

F

D

D

-

G

-

S

-

I

-

E

-

G

V

F

I

V

T

G

P

I

T

N

D

E

R

S

D

D

M

M

H

M

L

L

R

K

P

P

D

D

P

L

A

S

T

T

V

F

R

S

I

V

V

L

P

P

W

W

-
x
R

-

P

A

S

T

E

D

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

H

C

D

D

C

V

F

Y

D

T

R

T

D

K

G

G

R

K

L

P

V

F

P

E

F

G

A

D

P

P

R

R

S

G

V

C

L

L

R

K

R

R

V

V

C

M

D

E

L

E

Y

F

A

K

A

E

-

F

G

N

L

G

E

E

P

Y

V

F

V

V

A

G

P

P

E
|
E

L

P

E

E

F

F

Y

F

L

L

V

L

A

K

R

K

N

-

T

-

D

D

P

P

D

H

M

N

P

P

L

H

K

K

P

Y

P

I

I

P

G

A

R

D

S

D

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A

Y

Y

F

S

D

I

L

D

E

A

P

V

M

N

D

E

E

F

A

D
x
P

P

D

L

I

F
x
R

E
x
R

D

D

V

I

Y

V

D

F

Y

A

C

L

E

E

K

N

M

L

E

G

L

F

N

H

V

V

D
x
E

T

A

L
x
S

I

H

H

H

E

E

I

V

G

A

A

P

G

G

Q

Q

M

H

E

E

I

V

N

D

F
|
F

F
x
K

H
x
F

A

D

H

D

P

A

L

L

G

K

L

T

A

A

D

D

E

S

V

V

F

I

F

T

F

F

K

K

R

T

T

T

V

I

R

K

E

T

A

I

A

A

M

E

R

Q

H

H

D

G

M

L

F

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

F

A

F

G

G

E

M

P

N

G

G

S

S

A

G

M

M

H

H

I

C

H
|
H

Q

Q

S
|
S

V

I

L

W

R

L

K

N

D

G

T

E

G

P

L

S

N

F

I

Y

F

D

S

E

N

N

A

A

D

P

G

Y

S

Q

P

L

S

S

R

E

E

T

F

C

Y

M

W

N

Y

Y

I

V

G

A

G

G

L

I

Q

L

K

K

Y

H

V

A

P

K

A

A

A

I

M

V

A

A

L

I

F

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

A

V

R

P

F

G

T

Y

A

E

A

A

P

P

I

V

N

N

I

I

Q

A

W

W

G

A

T

N

D

S

N

N

R

R

T

S

V

A

G

I

I

I

R
|
R

S

V

P

P

V

A

A

A

P

R

P

G

A

K

A

G

R

T

R
|
R

V

I

E

E

N

F

R

R

V

A

V

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

T

A

V

T

M

L

L

A

A

C

A

G

G

W

L

L

D

G

G

I

V

K

K

N

N

R

K

I

L

E

D

P

A

T

P

P

E

E

P

C

V

K

E

G

R

D

N

A

I

Y

F

-

A

-

M

-

S

-

E

-

A

-

E

-

K

-

E

L

L

G

G

D

I

Y

E

A

S

L

V

P

P

R

A

S

N

L

L

G

K

E

A

A

A

L

L

E

D

K

E

L

L

R

E

A

N

E

N

E

D

A

V

L

L

A

K

E

N

V

A

L

L

G

G

E

K

E

H

F

I

V

F

T

E

V

S

Y

F

T

L

E

E

V

I

K

K

E

N

T

A

E

E

F

W

A

D

E

S

F

F

M

R

K

T

V

S

I

V

S

T

P

D

W

W

E

E

binding 2-oxoglutaric acid: R87 (vs. gap), V93 (≠ T102), P170 (≠ D186), R173 (≠ F189), R174 (≠ E190), S190 (≠ L206)
binding adenosine-5'-triphosphate: E136 (= E144), E188 (≠ D204), F203 (= F219), K204 (≠ F220), F205 (≠ H221), H251 (= H267), S253 (= S269), R325 (= R341), R335 (= R351)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19, 33

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
31% identity, 84% coverage: 68:447/455 of query aligns to 50:439/446 of 8ooqB

query
sites
8ooqB

E

E

E

N

G

G

P

L

Y

Y

Y

F

D

D

-

G

-

S

-

I

-

E

-

G

V

F

I

V

T

G

P

I

T

N

D

E

R

S

D

D

M

M

H

M

L

L

R

K

P

P

D

D

P

L

A

S

T

T

V

F

R

S

I

V

V

L

P

P

W

W

-
x
R

-

P

A

S

T

E

D

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

H

C

D

D

C

V

F

Y

D

T

R

T

D

K

G

G

R

K

L

P

V

F

P

E

F

G

A

D

P

P

R

R

S

G

V

C

L

L

R

K

R

R

V

V

C

M

D

E

L

E

Y

F

A

K

A

E

-

F

G

N

L

G

E

E

P

Y

V

F

V

V

A

G

P

P

E

E

L

P

E
|
E

F

F

Y

F

L

L

V

L

A

K

R

K

N

-

T

-

D

D

P

P

D

H

M

N

P

P

L

H

K

K

P

Y

P

I

I

P

G

A

R

D

S

D

G

G

R

-

A

-

E

-

T

-

S

-

R

-

Q

G

A

Y

Y

F

S

D

I

L

D

E

A

P

V

M

N

D

E

E

F

A

D
x
P

P

D

L

I

F
x
R

E
x
R

D

D

V

I

Y

V

D

F

Y

A

C

L

E

E

K

N

M

L

E

G

L

F

N

H

V

V

D

E

T

A

L
x
S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

V

N

D

F

F

F

K

H

F

A

D

H

D

P

A

L

L

G

K

L

T

A

A

D

D

E

S

V

V

F

I

F

T

F

F

K

K

R

T

T

T

V

I

R

K

E

T

A

I

A

A

M

E

R

Q

H

H

D

G

M

L

F

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

F

A

F

G

G

E

M

P

N

G

G

S

S

A

G

M

M

H

H

I

C

H

H

Q

Q

S

S

V

I

L

W

R

L

K

N

D

G

T

E

G

P

L

S

N

F

I

Y

F

D

S

E

N

N

A

A

D

P

G

Y

S

Q

P

L

S

S

R

E

E

T

F

C

Y

M

W

N

Y

Y

I

V

G

A

G

G

L

I

Q

L

K

K

Y

H

V

A

P

K

A

A

A

I

M

V

A

A

L

I

F

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

A

V

R

P

F

G

T

Y

A

E

A

A

P

P

I

V

N

N

I

I

Q

A

W

W

G

A

T

N

D

S

N

N

R

R

T

S

V

A

G

I

I

I

R

R

S

V

P

P

V

A

A

A

P

R

P

G

A

K

A

G

R

T

R

R

V

I

E

E

N

F

R

R

V

A

V

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

V

L

A

A

L

F

A

T

A

V

T

M

L

L

A

A

C

A

G

G

W

L

L

D

G

G

I

V

K

K

N

N

R

K

I

L

E

D

P

A

T

P

P

E

E

P

C

V

K

E

G

R

D

N

A

I

Y

F

-

A

-

M

-

S

-

E

-

A

-

E

-

K

-

E

L

L

G

G

D

I

Y

E

A

S

L

V

P

P

R

A

S

N

L

L

G

K

E

A

A

A

L

L

E

D

K

E

L

L

R

E

A

N

E

N

E

D

A

V

L

L

A

K

E

N

V

A

L

L

G

G

E

K

E

H

F

I

V

F

T

E

V

S

Y

F

T

L

E

E

V

I

K

K

E

N

T

A

E

E

F

W

A

D

E

S

F

F

M

R

K

T

V

S

I

V

S

T

P

D

W

W

E

E

binding 2-oxoglutaric acid: R86 (vs. gap), V92 (≠ T102), P169 (≠ D186), R172 (≠ F189), R173 (≠ E190), S189 (≠ L206)
binding magnesium ion: E137 (= E146), E192 (= E209), E199 (= E216)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18, 32

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
34% identity, 80% coverage: 85:447/455 of query aligns to 56:390/396 of 5dm3C

query
sites
5dm3C

L

M

R

K

P

P

D

D

P

L

A

A

T

T

V

L

R

R

I

C

V

V

P

P

W

W

A

-

T

L

D

E

P

G

T

T

A

A

Q

M

V

V

I

L

H

C

D

D

C

L

F

L

D

D

-

H

R

R

D

T

G

H

R

A

L

E

V

V

P

P

F

H

A

A

P

P

R

R

S

A

V

I

L

L

R

K

R

R

V

Q

C

L

D

A

L

R

Y

L

A

E

A

A

A

M

G

G

L

L

E

E

P

A

V

I

V

M

A

A

P

T

E
|
E

L

L

E
|
E

F

F

Y

F

L

L

V

F

A

E

R

K

N

S

T

L

D

D

P

E

D

I

M

-

P

-

L

-

K

-

P

-

I

-

G

-

R

R

S

K

G

G

R

R

A

F

E

R

T

T

S

T

R

K

Q

E

A

E

Y

H

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

-

P

-

L

V

F

L

E

R

D

P

V

L

Y

R

D

N

Y

H

C

L

E

H

K

A

M

A

E

G

L

I

N

P

V

V

D

E

T

G

L

T

I

K

H

G

E
|
E

I

A

G

G

A

A

G

G

Q

Q

M

E

E
|
E

I

L

N

N

F

I

F
x
R

H
x
C

A

A

H

K

P

A

L

L

G

D

L

T

A

A

D

D

E

Y

V

H

F

T

F

I

F

A

K

K

R

H

T

A

V

T

R

K

E

E

A

I

A

A

M

W

R

Q

H

Q

D

G

M

R

F

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

I

H

A

H

G

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

I

I

H
|
H

Q

Q

S
|
S

V

L

L

W

R

K

K

Q

D

-

T

-

G

G

L

L

N

P

I

A

F

F

S

H

N

D

A

E

D

R

G

D

S

A

-

L

-

G

P

M

S

S

R

A

E

L

F

M

Y

K

W

H

Y

Y

I

L

G

A

G

G

L

L

Q

L

K

K

Y

Y

V

A

P

P

A

D

A

Y

M

T

A

Y

L

F

F

L

A

A

P

P

Y

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

L

-

A

-

R

-

F

F

T

Q

A

F

A

A

P

P

I

T

N

R

I

T

Q

V

W

W

G

S

T

V

D

D

N

N

R
|
R

T

T

V

A

G

G

I

F

R

R

S

L

P

C

V

A

A

E

P

G

P

T

A

R

A

A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

V

I

V

G

G

G

A

S

D

D

A

L

N

N

P

P

Y

Y

V

L

A

A

L

M

A

A

A

G

T

Q

L

L

A

A

C

A

G

G

W

I

L

K

G

G

I

I

K

E

N

E

R

C

I

L

E

A

P

L

T

P

P

P

E

P

C

A

K

S

G

G

D

-

A

-

Y

-

L

-

G

-

D

-

Y

-

A

L

L

I

P

P

R

Q

S

N

L

L

G

R

E

D

A

A

L

M

E

E

K

A

L

L

R

R

A

G

E

S

E

T

A

M

L

L

A

R

E

E

V

A

L

M

G

G

E

E

E

D

F

V

V

V

T

D

V

H

Y

Y

T

V

E

R

V

A

K

A

E

E

T

V

E

E

F

L

A

E

E

D

F

F

M

Q

K

R

V

V

I

V

S

S

P

D

W

Y

E

E

active site: E115 (= E144), E117 (= E146), E162 (= E209), E169 (= E216), H218 (= H265), R286 (= R336), E303 (= E353), R305 (= R355)
binding adenosine-5'-diphosphate: R173 (≠ F220), C174 (≠ H221), H220 (= H267), S222 (= S269), R301 (= R351)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 82% coverage: 79:453/455 of query aligns to 63:446/446 of P9WN37

query
sites
P9WN37

T

S

D

E

R

S

D

D

M

T

H

V

L

A

R

H

P

P

D

D

P

P

A

S

T

T

V

F

R

Q

I

V

V

L

P

P

W

W

A

A

T

T

D

S

P

S

-

G

-

H

T

S

A

A

Q

R

V

M

I

F

H

C

D

D

C

I

F

T

D

M

R

P

D

D

G

G

R

S

L

P

V

S

P

W

F

A

A

D

P

P

R

R

S

H

V

V

L

L

R

R

V

Q

C

L

D

T

L

K

Y

A

A

G

A

E

A

L

G

G

L

F

E

S

P

C

V

Y

V

V

A

H

P

P

E

E

L

I

E

E

F

F

Y

F

L

L

V

L

A

K

R

P

N

G

T

P

D

E

P

-

D

D

M

G

P

S

L

V

K

P

P

V

P

P

I

V

G

D

R

N

S

A

G

G

R

Y

A

F

E

D

T

-

S

-

R

-

Q

Q

A

A

Y

V

S

H

I

D

D

S

A

A

V

L

N

N

E

-

F

-

D

-

P

-

L

F

F

R

E

R

D

H

V

A

Y

I

D

D

Y

A

C

L

E

E

K

F

M

M

E

G

L

I

N

S

V

V

D

E

T

F

L

S

I

H

H

H

E

E

I

G

G

A

A

P

G

G

Q

Q

M

Q

E

E

I

I

N

D

F

L

F

R

H

F

A

A

H

D

P

A

L

L

G

S

L

M

A

A

D

D

E

N

V

V

F

M

F

T

F

F

K

R

R

Y

T

V

V

I

R

K

E

E

A

V

A

A

M

L

R

E

H

E

D

G

M

A

F

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

F

A

G

G

Q

E

H

P

P

G

G

S

S

A

A

M

M

H

H

I

T

H

H

Q

M

S

S

V

L

L

F

R

E

K

G

D

D

T

-

G

-

L

V

N

N

I

A

F

F

S

H

N

S

A

A

D

D

G

D

-

P

-

L

S

Q

P

L

S

S

R

E

E

V

F

G

Y

K

W

S

Y

F

I

I

G

A

G

G

L

I

Q

L

K

E

Y

H

V

A

P

C

A

E

A

I

M

S

A

A

L

V

F

T

A

N

P

Q

Y

W

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

A

V

R

Q

F

G

T

G

A

E

A

A

P

P

I

T

N

A

I

A

Q

S

W

W

G

G

T

A

D

A

N

N

R

R

T

S

V

A

G

L

I

V

R

R

S

V

P

P

V

M

A

Y

P

T

P

P

-

H

-

K

-

T

A

S

R

R

V

V

E

E

N

V

R

R

V

S

V

P

G

D

A

S

D

A

A

C

N

N

P

P

Y

Y

V

L

A

T

L

F

A

A

A

V

T

L

L

L

A

A

C

A

G

G

W

L

L

R

G

G

I

V

K

E

N
x
K

R

G

I

Y

E

V

P

L

T

G

P

P

E

Q

C

A

K

E

G

D

D

N

A

V

Y

W

-

D

-

L

-

T

-

P

-

E

-

R

-

A

L

M

G

G

D

Y

Y

R

A

E

L

L

P

P

R

S

S

S

L

L

G

D

E

S

A

A

L

L

E

R

K

A

L

M

R

E

A

A

E

S

E

E

A

L

L

V

A

A

E

E

V

A

L

L

G

G

E

E

E

H

F

V

V

F

T

D

V

F

Y

F

T

L

E

R

V

N

K

K

E

R

T

T

E

E

F

W

A

A

E

N

F

Y

M

R

K

S

V

H

I

V

S

T

P

P

W

Y

E

E

-

L

R

R

E

T

H

Y

L

L

L

S

L

L

K363 (≠ N380) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
31% identity, 82% coverage: 80:452/455 of query aligns to 64:444/446 of A0R083

query
sites
A0R083

D

E

R

S

D

D

M

T

H

V

L

A

R

R

P

P

D

D

P

P

A

S

T

T

V

F

R

Q

I

V

V

L

P

P

W

W

A

K

T

T

D

S

P

D

-

G

-

N

-

H

-

Y

T

S

A

A

Q

R

V

M

I

F

H

C

D

D

C

I

F

T

D

M

R

P

D

D

G

G

R

S

L

P

V

S

P

W

F

A

A

D

P

S

R

R

S

H

V

V

L

L

R

R

V

Q

C

L

D

A

L

K

Y

A

A

S

A

D

A

L

G

G

L

F

E

T

P

C

V

Y

V

V

A

H

P

P

E

E

L

I

E

E

F

F

Y

F

L

L

V

L

A

K

R

P

N

G

T

P

D

N

P

D

D

G

M

T

P

P

L

P

K

E

P

P

P

-

I

-

G

-

R

-

S

-

G

-

R

-

A

-

E

-

T

A

S

D

R

N

Q

G

A

G

Y

Y

S

F

I

D

D

Q

A

A

V

V

N

H

E

D

F

A

D

A

P

P

L

N

F

F

E

R

-

R

D

H

V

A

Y

I

D

E

Y

A

C

L

E

E

K

Q

M

M

E

G

L

I

N

S

V

V

D

E

T

F

L

S

I

H

H

H

E

E

I

G

G

A

A

P

G

G

Q

Q

M

Q

E

E

I

I

N

D

F

L

F

R

H

Y

A

A

H

D

P

A

L

L

G

S

L

M

A

A

D

D

E

N

V

V

F

M

F

T

F

F

K

R

R

Y

T

L

V

V

R

K

E

E

A

V

A

A

M

L

R

A

H

D

D

G

M

V

F

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

F

A

A

G

E

E

H

P

P

G

G

S

S

A

A

M

M

H

H

I

T

H

H

Q

M

S

S

V

L

L

F

R

E

K

G

D

D

T

T

G

-

L

-

N

N

I

A

F

F

S

H

N

S

A

P

D

D

G

D

-

P

-

L

S

Q

P

L

S

S

R

D

E

V

F

A

Y

K

W

S

Y

F

I

I

G

A

G

G

L

I

Q

L

K

E

Y

H

V

A

P

N

A

E

A

I

M

S

A

A

L

V

F

T

A

N

P

Q

Y

W

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

A

V

R

H

F

G

T

G

A

E

A

A

P

P

I

T

N

A

I

A

Q

S

W

W

G

G

T

A

D

A

N

N

R

R

T

S

V

A

G

L

I

V

R

R

S

V

P

P

V

M

A

Y

P

T

P

P

-

H

-

K

-

V

A

S

R

R

V

V

E

E

N

V

R

R

V

S

V

P

G

D

A

S

D

A

A

C

N

N

P

P

Y

Y

V

L

A

T

L

F

A

A

A

V

T

L

L

L

A

A

C

A

G

G

W

L

L

R

G

G

I

V

K

E

N
x
K

R

G

I

Y

E

V

P

L

T

G

P

P

E

Q

C

A

K

E

G

D

D

N

A

V

Y

W

-

S

-

L

-

T

-

Q

-

E

-

R

-

A

L

M

G

G

D

Y

Y

R

A

E

L

L

P

P

R

T

S

S

L

L

G

G

E

N

A

A

L

L

E

E

K

S

L

M

R

E

A

N

E

S

E

E

A

L

L

V

A

A

E

E

V

A

L

L

G

G

E

E

E

H

F

V

V

F

T

D

V

Y

Y

F

T

L

E

R

V

N

K

K

E

R

T

S

E

E

F

W

A

E

E

N

F

Y

M

R

K

S

V

H

I

V

S

T

P

P

W

Y

E

E

R

L

E

K

H

N

L

Y

L

L

K363 (≠ N380) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tenA Glutamine synthetase (see paper)
28% identity, 98% coverage: 7:452/455 of query aligns to 2:439/442 of 7tenA

query
sites
7tenA

F

Y

T

T

Y

K

S

E

D

D

L

I

E

F

H

R

W

F

L

A

D

D

Q

E

H

Q

R

N

V

V

T

K

E

F

I

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

I

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

Q

S

K

Q

F

L

T

K

E

K

D

-

R

-

G

-

M

-

R

A

L

L

P

D

E

N

I

K

V

I

V

M

A

F

M

D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

G

-

P

-

Y

-

D

G

V

F

I

V

T

R

P

I

T

E

D

E

R

S

D

D

M

M

H

Y

L

L

R

F

P

P

D

D

P

L

A

D

T

T

V

W

R

V

I

V

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

M

I

I

H

C

D

D

C

I

F

Y

D

N

R

P

D

D

G

-

R

-

L

M

V

T

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

A

V

N

L

L

R

K

R

R

V

V

C

L

D

K

L

E

Y

M

A

E

A

L

G

G

L

F

-

T

E

E

P

F

V

N

V

L

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

N

T

R

D

R

P

P

D

T

M

L

P

E

L

L

K

N

P

D

P

S

I

G

G

G

R

Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F
|
F

F
x
K

H
x
Y

A

E

H

D

P

A

L

I

G

T

L

A

A

C

D

D

E

S

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

K

H

H

D

G

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H
x
N

Q

M

S
|
S

V

L

L

F

R

N

K

E

D

K

T

G

G

N

L

A

N

F

I

F

F

D

S

E

N

S

A

G

D

E

G

L

S

E

P

L

S

S

R

Q

E

T

F

A

Y

Y

W

H

Y

F

I

L

G

A

G

G

L

M

Q

L

K

K

Y

H

V

A

P

R

A

G

A

Y

M

T

A

A

L

V

F

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

A

V

R

P

F

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

I

Q

A

W

W

G

S

T

G

D

K

N

N

R
|
R

T

S

V

P

G

L

I

V

R
|
R

S

V

P

P

V

S

A

S

P

R

P

G

A

L

A
x
S

R

T

R
|
R

V

L

E

E

N

L

R
|
R

V

S

V

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

V

L

A

A

L

M

A

A

A

V

T

L

L

L

A

K

C

A

G

G

W

L

L

S

G

G

I

I

K

K

N

D

R

E

I

L

E

T

P

P

T

P

P

A

E

P

C

V

K

D

G

R

D

N

A

I

Y

Y

-

G

-

M

-

N

-

E

-

R

-

E

-

A

L

T

G

G

D

I

Y

Y

A

D

L

L

P

P

R

E

S

S

L

L

G

G

E

H

A

A

L

L

E

I

K

E

L

L

R

E

A

K

E

N

E

E

A

I

L

I

A

K

E

D

V

G

L

L

G

G

E

E

E

H

F

I

V

F

T

E

V

H

Y

F

T

I

E

E

V

A

K

K

E

T

T

I

E

E

F

C

A

D

E

M

F

F

M

R

K

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A142), E130 (= E144), E182 (≠ D204), D196 (≠ N218), F197 (= F219), K198 (≠ F220), Y199 (≠ H221), N245 (≠ H267), S247 (= S269), R319 (= R341), S327 (≠ A349), R329 (= R351)
binding l-methionine-s-sulfoximine phosphate: E130 (= E144), E132 (= E146), E187 (= E209), E194 (= E216), N238 (≠ P260), G239 (= G261), H243 (= H265), R296 (= R318), E302 (≠ A324), R314 (= R336), R333 (= R355)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
28% identity, 98% coverage: 7:452/455 of query aligns to 3:440/443 of 7tf9S

query
sites
7tf9S

F

Y

T

T

Y

K

S

E

D

D

L

I

E

F

H

R

W

F

L

A

D

D

Q

E

H

Q

R

N

V

V

T

K

E

F

I

I

E

R

C

L

L

Q

V

F

P

T

D

D

L

I

T

L

G

G

V

I

A

I

R

K

G

N

K

V

I

E

L

I

P

P

R

V

Q

S

K

Q

F

L

T

K

E

K

D

-

R

-

G

-

M

-

R

A

L

L

P

D

E

N

I

K

V

I

V

M

A

F

M

D

G

G

V

S

T

S

G

I

E

E

F

-

P

-

E

-

G

-

P

-

Y

-

D

G

V
x
F

I
x
V

T

R

P

I

T

E

D

E

R

S

D

D

M

M

H

Y

L

L

R

F

P

P

D

D

P

L

A

D

T

T

V

W

R

V

I

V

V

F

P

P

W

W

A

T

T

A

D

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

M

I

I

H

C

D

D

C

I

F

Y

D

N

R

P

D

D

G

-

R

-

L

M

V

T

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

A

V

N

L

L

R

K

R

R

V

V

C

L

D

K

L

E

Y

M

A

E

A

L

G

G

L

F

-

T

E

E

P

F

V

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

N

T

R

D

R

P

P

D

T

M

L

P

E

L

L

K

N

P

D

P

S

I

G

G

G

R
x
Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

Y

E

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

E

H

D

P

A

L

I

G

T

L

A

A

C

D

D

E

S

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

K

H

H

D

G

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

F

H

N

Q

M

S

S

V

L

L

F

R

N

K

E

D

K

T

G

G

N

L

A

N

F

I

F

F

D

S

E

N

S

A

G

D

E

G

L

S

E

P

L

S

S

R

Q

E

T

F

A

Y

Y

W

H

Y

F

I

L

G

A

G

G

L

M

Q

L

K

K

Y

H

V

A

P

R

A

G

A

Y

M

T

A

A

L

V

F

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

A

V

R

P

F

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

I

Q

A

W

W

G

S

T

G

D

K

N

N

R

R

T

S

V

P

G

L

I

V

R

R

S

V

P

P

V

S

A

S

P

R

P

G

A

L

A

S

R

T

R

R

V

L

E
|
E

N

L

R

R

V

S

V

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

V

L

A

A

L

M

A

A

A

V

T

L

L

L

A

K

C

A

G

G

W

L

L

S

G

G

I

I

K

K

N

D

R

E

I

L

E

T

P

P

T

P

P

A

E

P

C

V

K

D

G

R

D

N

A

I

Y

Y

-

G

-

M

-

N

-

E

-

R

-

E

-

A

L

T

G

G

D

I

Y

Y

A

D

L

L

P

P

R

E

S

S

L

L

G

G

E

H

A

A

L

L

E

I

K

E

L

L

R

E

A

K

E

N

E

E

A

I

L

I

A

K

E

D

V

G

L

L

G

G

E

E

E

H

F

I

V

F

T

E

V

H

Y

F

T

I

E
|
E

V

A

K

K

E

T

T
x
I

E

E

F

C

A

D

E
x
M

F

F

M

R

K

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

L

binding glutamine: E133 (= E146), Y155 (≠ R166), E188 (= E209), G240 (= G261), G242 (≠ A263), R297 (= R318), E303 (≠ A324)
binding magnesium ion: E131 (= E144), E133 (= E146), E188 (= E209), E195 (= E216), H244 (= H265), E332 (= E353)
binding : F59 (≠ V75), V60 (≠ I76), E418 (= E430), I422 (≠ T434), M426 (≠ E438)

7tfaB Glutamine synthetase (see paper)
31% identity, 82% coverage: 80:452/455 of query aligns to 63:438/441 of 7tfaB

query
sites
7tfaB

D

E

R

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

A

D

T

T

V

W

R

V

I

V

V

F

P

P

W

W

A

V

T

T

-

S

D

D

P

R

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

Y

D

K

R

P

D

D

G

G

R

S

L

-

V

-

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

G

V

I

L

L

R

K

R

R

V

V

C

L

D

K

L

E

Y

A

A

E

A

L

G

G

L

Y

E

T

P

S

V

M

-

N

V

V

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

R

T

N

D

T

E

-

K

-

G

D

D

P

P

D

T

M

T

P

E

L

L

K

N

P

D

P

Q

I

G

G

G

R

-

S

-

G

-

R

-

A

-

E

-

T

-

S

-

R

-

Q

-

A
x
Y

Y

F

S

D

I

L

D

A

A

P

V

M

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

E

V

I

Y

V

D

L

Y

K

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

I
x
V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

A

H

D

P

A

L

V

G

K

L

A

A

A

D

D

E

Q

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

Q

H

H

D

G

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

Q

S

S

V

L

L

F

R

K

K

D

D

N

T

E

G

N

L

V

N

F

I

Y

F

D

S

E

N

T

A

D

D

E

G

L

S

G

P

L

S

S

R

Q

E

T

F

A

Y

R

W

H

Y

Y

I

M

G

A

G

G

L

I

Q

L

K

K

Y

H

V

A

P

R

A

A

A

M

M

A

A

A

L

I

F

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

A

V

R

P

F
x
G

T
x
Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

Q

A

W

W

G

S

T

A

D

S

N

N

R
|
R

T

S

V

P

G

M

I

I

R

R

S

I

P

P

V

A

A

S

P

R

P

G

A

L

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

V

N

V

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

A

A

V

T

M

L

L

A

R

C

A

G

G

W

L

L

D

G

G

I

I

K

K

N

R

R

Q

I

M

E

A

P

L

T

P

P

A

E

P

C

I

K

D

G

R

D

N

A

I

Y

Y

L

V

-

M

-

S

-

E

-

R

-

I

-

E

G

G

D

I

Y

P

A

S

L

L

P

P

R

A

S

D

L

L

G

K

E

E

A

A

L

L

E

S

K

E

L

L

R

I

A

R

E

S

E

E

A

V

L

I

A

S

E

D

V

A

L

L

G

G

E

D

E

H

F

A

V

L

T

A

V

Y

Y

F

T

Y

E

E

V

L

K

K

E

E

T

I

E

E

F

W

A

D

E
x
M

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

R

E

D

H

Q

L

Y

L

L

binding glutamine: E131 (= E146), Y153 (≠ A176), E186 (= E209), G238 (= G261), H242 (= H265), R295 (= R318), E301 (≠ A324)
binding magnesium ion: E129 (= E144), E131 (= E146), E186 (= E209), E193 (= E216), H242 (= H265), E330 (= E353)
binding : V187 (≠ I210), N237 (≠ P260), G299 (≠ F322), Y300 (≠ T323), R313 (= R336), M424 (≠ E438)

Sites not aligning to the query:

binding : 58, 60

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
31% identity, 82% coverage: 80:452/455 of query aligns to 63:436/439 of 7tdpA

query
sites
7tdpA

D

E

R

S

D

D

M

M

H

Y

L

L

R

Y

P

P

D

D

P

L

A

D

T

T

V

W

R

V

I

V

V

F

P

P

W

W

A

-

T

S

D

D

P

R

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

I

F

Y

D

K

R

P

D

D

G

G

R

S

L

-

V

-

P

P

F

F

A

A

-

G

-

D

P

P

R

R

S

G

V

I

L

L

R

K

R

R

V

V

C

L

D

K

L

E

Y

A

A

E

A

L

G

G

L

Y

E

T

P

S

V

M

-
x
N

V

V

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

V

F

A

K

R

T

N

D

T

E

-

K

-

G

D

D

P

P

D

T

M

T

P

E

L

L

K

N

P

D

P

Q

I

G

G

G

R

-

S

-

G

-

R

-

A

-

E

-

T

-

S

-

R

-

Q

-

A

Y

Y

F

S

D

I

L

D

A

A

P

V

M

N

D

E

L

F

G

D

E

P

N

L

C

F

R

E

R

D

E

V

I

Y

V

D

L

Y

K

C

L

E

E

K

E

M

M

E

G

L

F

N

E

V

I

D
x
E

T

A

L

S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N
x
D

F

F

F

K

H
x
Y

A

A

H

D

P

A

L

V

G

K

L

A

A

A

D

D

E

Q

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

Q

H

H

D

G

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H
x
N

Q

Q

S
|
S

V

L

L

F

R

K

K

D

D

N

T

E

G

N

L

V

N

F

I

Y

F

D

S

E

N

T

A

D

D

E

G

L

S

G

P

L

S

S

R

Q

E

T

F

A

Y

R

W

H

Y

Y

I

M

G

A

G

G

L

I

Q

L

K

K

Y

H

V

A

P

R

A

A

A

M

M

A

A

A

L

I

F

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

A

V

R

P

F

G

T

Y

A
x
E

A

A

P

P

I

C

N

Y

I

V

Q

A

W

W

G

S

T

A

D

S

N

N

R
|
R

T

S

V

P

G

M

I

I

R
|
R

S

I

P

P

V

A

A

S

P

R

P

G

A

L

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

N

V

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

L

A

A

L

L

A

A

A

V

T

M

L

L

A

R

C

A

G

G

W

L

L

D

G

G

I

I

K

K

N

R

R

Q

I

M

E

A

P

L

T

P

P

A

E

P

C

I

K

D

G

R

D

N

A

I

Y

Y

L

V

-

M

-

S

-

E

-

R

-

I

-

E

G

G

D

I

Y

P

A

S

L

L

P

P

R

A

S

D

L

L

G

K

E

E

A

A

L

L

E

S

K

E

L

L

R

I

A

R

E

S

E

E

A

V

L

I

A

S

E

D

V

A

L

L

G

G

E

D

E

H

F

A

V

L

T

A

V

Y

Y

F

T

Y

E

E

V

L

K

K

E

E

T

I

E

E

F

W

A

D

E

M

F

Y

M

R

K

T

V

Q

I

V

S

H

P

Q

W

W

E

E

R

R

E

D

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A142), E127 (= E144), E179 (≠ D204), D193 (≠ N218), Y196 (≠ H221), N242 (≠ H267), S244 (= S269), R316 (= R341), R326 (= R351)
binding magnesium ion: E127 (= E144), E127 (= E144), E129 (= E146), E184 (= E209), E191 (= E216), E191 (= E216), H240 (= H265), E328 (= E353)
binding l-methionine-s-sulfoximine phosphate: E127 (= E144), E129 (= E146), E184 (= E209), E191 (= E216), G236 (= G261), H240 (= H265), R293 (= R318), E299 (≠ A324), R311 (= R336), R330 (= R355)

7tf6A Glutamine synthetase (see paper)
30% identity, 82% coverage: 80:452/455 of query aligns to 63:435/438 of 7tf6A

query
sites
7tf6A

D

E

R

S

D

D

M

M

H

Y

L

L

R

H

P

P

D

D

P

L

A

D

T

T

V

W

R

V

I

I

V

F

P

P

W

W

A

G

T

K

D

-

P

-

T

V

A

A

Q

R

V

L

I

I

H

C

D

D

C

V

F

Y

D

K

R

T

D

D

G

G

R

T

L

P

V

F

P

E

F

G

A

D

P

P

R

R

S

A

V

N

L

L

R

K

R

R

V

V

C

L

D

K

L

E

Y

M

A

E

A

D

A

L

G

G

L

F

E

T

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

V

L

A

D

R

E

N

K

T

G

D

E

P

P

D

T

M

L

P

E

L

L

K

N

P

D

I

G

G

G

R

Y

S

F

G

D

R

L

A

A

E

P

T

T

S

D

R

L

Q

G

A

-

Y

-

S

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

N

L

C

F

R

E

R

D

D

V

I

Y

V

D

L

Y

E

C

L

E

E

K

D

M

M

E

G

L

F

N

D

V

I

D

E

T

A

L

S

I

H

H

H

E
|
E

I

V

G

A

A

P

G

G

Q

Q

M

H

E
|
E

I

I

N

D

F

F

F

K

H

Y

A

A

H

D

P

A

L

V

G

T

L

A

A

C

D

D

E

N

V

I

F

Q

F

T

F

F

K

K

R

L

T

V

V

V

R

K

E

T

A

I

A

A

M

R

R

K

H

H

D

N

M

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

G
|
G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H

N

Q

V

S

S

V

L

L

F

R

K

K

G

D

K

T

E

G

N

L

A

N

F

I

F

F

D

S

P

N

N

A

T

D

E

G

M

S

G

P

L

S

T

R

E

E

T

F

A

Y

Y

W

Q

Y

F

I

T

G

A

G

G

L

V

Q

L

K

K

Y

N

V

A

P

R

A

G

A

F

M

T

A

A

L

V

F

C

A

N

P

P

Y

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

A

V

R

P

F
x
G

T
x
Y

A
x
E

A

A

P

P

I

C

N

Y

I

I

Q

A

W

W

G

S

T

G

D

K

N

N

R
|
R

T

S

V

P

G

L

I

I

R

R

S

V

P

P

V

S

A

S

P

R

P

G

A

L

A

S

R

T

R

R

V

I

E
|
E

N

V

R

R

V

S

V

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

V

M

A

A

L

L

A

A

T

I

L

L

A

E

C

A

G

G

W

L

L

D

G

G

I

I

K

K

N

N

R

K

I

L

E

K

P

V

T

P

P

E

E

P

C

V

K

N

G

Q

D

N

A

I

Y
|
Y

-

E

-

M

-

N

-

R

-

E

-

R

-

E

-

A

L

V

G

G

D

I

Y

Q

A

D

L

L

P

P

R

S

S

T

L

L

G

Y

E

T

A

A

L

L

E

K

K

A

L

M

R

R

A

E

E

N

E

E

A

V

L

I

A

K

E

K

V

A

L

L

G

G

E

N

E

H

F

I

V

Y

T

N

V

Q

Y

F

T

I

E

N

V

S

K

K

E

S

T

I

E

E

F

W

A

D

E
x
Y

F

Y

M

R

K

T

V

Q

I

V

S

S

P

E

W

W

E

E

R

R

E

D

H
x
Q

L

Y

L

M

binding glutamine: E128 (= E146), E183 (= E209), G235 (= G261), H239 (= H265), R292 (= R318), E298 (≠ A324)
binding magnesium ion: E126 (= E144), E128 (= E146), E183 (= E209), E190 (= E216), H239 (= H265), E327 (= E353)
binding : G232 (= G258), N234 (≠ P260), G296 (≠ F322), Y297 (≠ T323), R310 (= R336), Y367 (= Y393), Y421 (≠ E438), Q433 (≠ H450)

Sites not aligning to the query:

binding : 58, 60, 437

Query Sequence

>WP_029001753.1 NCBI__GCF_000430725.1:WP_029001753.1
MAERKDFTYSDLEHWLDQHRVTEIECLVPDLTGVARGKILPRQKFTEDRGMRLPEIVVAM
GVTGEFPEEGPYYDVITPTDRDMHLRPDPATVRIVPWATDPTAQVIHDCFDRDGRLVPFA
PRSVLRRVCDLYAAAGLEPVVAPELEFYLVARNTDPDMPLKPPIGRSGRAETSRQAYSID
AVNEFDPLFEDVYDYCEKMELNVDTLIHEIGAGQMEINFFHAHPLGLADEVFFFKRTVRE
AAMRHDMFATFMAKPIAGEPGSAMHIHQSVLRKDTGLNIFSNADGSPSREFYWYIGGLQK
YVPAAMALFAPYVNSYRRLARFTAAPINIQWGTDNRTVGIRSPVAPPAARRVENRVVGAD
ANPYVALAATLACGWLGIKNRIEPTPECKGDAYLGDYALPRSLGEALEKLRAEEALAEVL
GEEFVTVYTEVKETEFAEFMKVISPWEREHLLLHV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory