SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_029132004.1 NCBI__GCF_000428045.1:WP_029132004.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
45% identity, 83% coverage: 5:91/105 of query aligns to 114:200/200 of 6j2lA

query
sites
6j2lA

L

L

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R

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L

L

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E

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L

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L

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binding zinc ion: E152 (= E43), E171 (= E62), D174 (= D65)

Sites not aligning to the query:

binding zinc ion: 79, 81, 83, 96, 103

6j2lB Crystal structure of bi-functional enzyme (see paper)
39% identity, 83% coverage: 5:91/105 of query aligns to 107:185/185 of 6j2lB

query
sites
6j2lB

L

L

E

Y

R

Q

L

L

A

E

E

Q

V

L

L

L

E

A

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-

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L

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binding magnesium ion: E137 (= E43), E140 (= E46), E156 (= E62), D159 (= D65)

Sites not aligning to the query:

binding zinc ion: 78, 80, 82

Query Sequence

>WP_029132004.1 NCBI__GCF_000428045.1:WP_029132004.1
MSDSLERLAEVLEARKNADPDSSYVAKLYSKGLDAILKKIGEEATETVMAAKDGDREKII
YETADLWFHSLVLLAHQGLKPQDVLDELDRRFGLSGLEEKARRGK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory