SitesBLAST
Comparing WP_029132299.1 NCBI__GCF_000428045.1:WP_029132299.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4iqdA Crystal structure of carboxyvinyl-carboxyphosphonate phosphorylmutase from bacillus anthracis
33% identity, 91% coverage: 3:272/296 of query aligns to 5:270/290 of 4iqdA
- active site: Y46 (≠ F44), S48 (= S46), G49 (= G47), A50 (≠ F48), D60 (= D59), D87 (= D86), D89 (= D88), Q114 (≠ M113), E116 (= E115), K122 (= K121), C124 (= C123), G125 (= G124), H126 (= H125), R157 (= R159), E187 (= E189), N209 (= N211)
- binding pyruvic acid: E71 (≠ D70), R72 (≠ Q71), D75 (≠ N74), G165 (= G167), L166 (≠ M168), Y218 (≠ L220), Y219 (≠ L221)
3fa3B Crystal structure of 2,3-dimethylmalate lyase, a pep mutase/isocitrate lyase superfamily member, trigonal crystal form (see paper)
39% identity, 92% coverage: 6:278/296 of query aligns to 4:282/302 of 3fa3B
- active site: Y43 (≠ F44), T45 (≠ S46), G46 (= G47), A47 (≠ F48), D58 (= D59), D86 (= D86), D88 (= D88), H113 (≠ M113), E115 (= E115), K121 (= K121), C123 (= C123), G124 (= G124), H125 (= H125), R160 (= R159), E190 (= E189), N213 (= N211), T220 (= T218), S222 (≠ L220)
- binding 2,2-difluoro-3,3-dihydroxybutanedioic acid: Y43 (≠ F44), T45 (≠ S46), G46 (= G47), A47 (≠ F48), D86 (= D86), G124 (= G124), R160 (= R159), E190 (= E189), N213 (= N211), P239 (= P237)
1zlpA Petal death protein psr132 with cysteine-linked glutaraldehyde forming a thiohemiacetal adduct (see paper)
37% identity, 91% coverage: 11:279/296 of query aligns to 4:276/284 of 1zlpA
- active site: F37 (= F44), S39 (= S46), G40 (= G47), Y41 (≠ F48), D52 (= D59), D80 (= D86), D82 (= D88), F107 (≠ M113), E109 (= E115), K115 (= K121), C117 (= C123), G118 (= G124), H119 (= H125), R152 (= R159), E182 (= E189), N204 (= N211), T211 (= T218), L213 (= L220)
- binding 5-hydroxypentanal: C117 (= C123), G118 (= G124), R152 (= R159), I206 (≠ V213)
- binding magnesium ion: D80 (= D86), K115 (= K121)
1zlpB Petal death protein psr132 with cysteine-linked glutaraldehyde forming a thiohemiacetal adduct (see paper)
37% identity, 91% coverage: 11:279/296 of query aligns to 4:276/285 of 1zlpB
- active site: F37 (= F44), S39 (= S46), G40 (= G47), Y41 (≠ F48), D52 (= D59), D80 (= D86), D82 (= D88), F107 (≠ M113), E109 (= E115), K115 (= K121), C117 (= C123), G118 (= G124), H119 (= H125), R152 (= R159), E182 (= E189), N204 (= N211), T211 (= T218), L213 (= L220)
- binding 5-hydroxypentanal: Y41 (≠ F48), C117 (= C123), R152 (= R159), I206 (≠ V213)
Q05957 Petal death protein; (R)-2-methylmalate lyase; D-citramalate lyase; Oxalacetic hydrolase; PSR132; EC 3.7.1.1; EC 4.1.3.- from Dianthus caryophyllus (Carnation) (Clove pink) (see 2 papers)
37% identity, 91% coverage: 11:279/296 of query aligns to 31:303/318 of Q05957
- D79 (= D59) mutation to A: Reduces catalytic efficiency 1000-fold.
- D107 (= D86) binding Mg(2+)
- D109 (= D88) binding Mg(2+)
- K142 (= K121) binding Mg(2+)
- C144 (= C123) mutation to A: Loss of catalytic activity.
Sites not aligning to the query:
- 1:3 modified: propeptide, Removed in mature form
3m0jA Structure of oxaloacetate acetylhydrolase in complex with the inhibitor 3,3-difluorooxalacetate (see paper)
40% identity, 89% coverage: 4:266/296 of query aligns to 2:273/297 of 3m0jA
- binding calcium ion: E218 (= E214), N219 (≠ Q215)
- binding 2,2-difluoro-3,3-dihydroxybutanedioic acid: Y44 (≠ F44), T46 (≠ S46), G47 (= G47), A48 (≠ F48), D88 (= D86), G126 (= G124), R162 (= R159), E192 (= E189), N215 (= N211), S241 (≠ P237)
Q56062 2-methylisocitrate lyase; 2-MIC; MICL; (2R,3S)-2-methylisocitrate lyase; EC 4.1.3.30 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
35% identity, 95% coverage: 6:286/296 of query aligns to 5:280/295 of Q56062
- SGG 45:47 (≠ SGF 46:48) binding substrate
- D58 (= D59) mutation to A: Inactive. Retains the same oligomeric state of the wild-type.
- D85 (= D86) binding Mg(2+)
- K121 (= K121) mutation to A: 1000-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
- R122 (= R122) mutation to K: 2-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
- C123 (= C123) mutation to A: Inactive. Retains the same oligomeric state of the wild-type.
- H125 (= H125) mutation to A: 750-fold decrease in the catalytic efficiency. Retains the same oligomeric state of the wild-type.
- R158 (= R159) binding substrate
3m0kA Structure of oxaloacetate acetylhydrolase in complex with the product oxalate (see paper)
39% identity, 89% coverage: 4:266/296 of query aligns to 2:268/289 of 3m0kA
3fa4A Crystal structure of 2,3-dimethylmalate lyase, a pep mutase/isocitrate lyase superfamily member, triclinic crystal form (see paper)
37% identity, 92% coverage: 6:278/296 of query aligns to 4:275/284 of 3fa4A
- active site: Y43 (≠ F44), T45 (≠ S46), G46 (= G47), A47 (≠ F48), D58 (= D59), D86 (= D86), D88 (= D88), H113 (≠ M113), E115 (= E115), R153 (= R159), E183 (= E189), N206 (= N211), T213 (= T218), S215 (≠ L220)
- binding magnesium ion: D86 (= D86), D88 (= D88)
P77541 2-methylisocitrate lyase; 2-MIC; MICL; (2R,3S)-2-methylisocitrate lyase; EC 4.1.3.30 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 94% coverage: 6:284/296 of query aligns to 5:287/296 of P77541
- SGG 45:47 (≠ SGF 46:48) binding substrate
- D85 (= D86) binding Mg(2+)
- D87 (= D88) binding Mg(2+)
- C123 (= C123) mutation to S: Inactive.
- CG 123:124 (= CG 123:124) binding substrate
- R158 (= R159) binding substrate
- E188 (= E189) binding substrate
- NIT 210:212 (≠ NMV 211:213) binding substrate
- R241 (≠ S242) binding substrate
- R270 (≠ F267) binding substrate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1mumA Structure of the 2-methylisocitrate lyase (prpb) from escherichia coli (see paper)
33% identity, 94% coverage: 6:284/296 of query aligns to 3:285/289 of 1mumA
- active site: Y41 (≠ F44), S43 (= S46), G44 (= G47), G45 (≠ F48), D56 (= D59), D83 (= D86), D85 (= D88), H111 (≠ M113), E113 (= E115), K119 (= K121), C121 (= C123), G122 (= G124), H123 (= H125), R156 (= R159), E186 (= E189), N208 (= N211), T215 (= T218), L217 (= L220)
- binding magnesium ion: D56 (= D59), D85 (= D88)
3fa3J Crystal structure of 2,3-dimethylmalate lyase, a pep mutase/isocitrate lyase superfamily member, trigonal crystal form (see paper)
37% identity, 92% coverage: 6:278/296 of query aligns to 3:273/292 of 3fa3J
- active site: Y42 (≠ F44), T44 (≠ S46), G45 (= G47), A46 (≠ F48), D57 (= D59), D85 (= D86), D87 (= D88), H112 (≠ M113), E114 (= E115), R151 (= R159), E181 (= E189), N204 (= N211), T211 (= T218), S213 (≠ L220)
- binding manganese (ii) ion: D85 (= D86), D87 (= D88)
1o5qA Crystal structure of pyruvate and mg2+ bound 2-methylisocitrate lyase (prpb) from salmonella typhimurium (see paper)
33% identity, 95% coverage: 6:286/296 of query aligns to 1:265/271 of 1o5qA
- active site: Y39 (≠ F44), S41 (= S46), G42 (= G47), G43 (≠ F48), D54 (= D59), D81 (= D86), D83 (= D88), H109 (≠ M113), E111 (= E115), R143 (= R159), E173 (= E189), N195 (= N211), T202 (= T218), L204 (= L220)
- binding pyruvic acid: Y39 (≠ F44), S41 (= S46), G43 (≠ F48), D81 (= D86), R143 (= R159)
6t4vC Crystal structure of 2-methylisocitrate lyase (prpb) from pseudomonas aeruginosa in apo form.
32% identity, 88% coverage: 6:265/296 of query aligns to 3:251/277 of 6t4vC
- active site: Y41 (≠ F44), S43 (= S46), G44 (= G47), G45 (≠ F48), D56 (= D59), D83 (= D86), D85 (= D88), H111 (≠ M113), E113 (= E115), R145 (= R159), E175 (= E189), N197 (= N211), T204 (= T218), L206 (= L220)
- binding pyruvic acid: F88 (≠ Y91), N94 (= N96)
5uncA The crystal structure of phosphoenolpyruvate phosphomutase from streptomyces platensis subsp. Rosaceus
29% identity, 94% coverage: 8:286/296 of query aligns to 3:285/289 of 5uncA
- active site: W39 (≠ F44), S41 (= S46), G42 (= G47), L43 (≠ F48), D53 (= D59), D80 (= D86), D82 (= D88), T107 (≠ M113), E109 (= E115), K115 (= K121), N117 (≠ C123), S118 (≠ G124), R153 (= R159), H184 (≠ E189), V209 (= V213)
- binding alpha-D-xylopyranose: H22 (≠ F27), N23 (≠ D28), G26 (≠ S31), L29 (= L34), G239 (≠ A244), V243 (≠ M248)
Q84G06 Phosphonopyruvate hydrolase; PPH; EC 3.11.1.3 from Variovorax sp. (strain Pal2) (see paper)
32% identity, 84% coverage: 11:259/296 of query aligns to 7:257/290 of Q84G06