SitesBLAST
Comparing WP_029132301.1 NCBI__GCF_000428045.1:WP_029132301.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8dyjB Crystal structure of human methylmalonyl-coa mutase in complex with adp and cob(ii)alamin (see paper)
65% identity, 99% coverage: 6:712/716 of query aligns to 1:705/708 of 8dyjB
- binding adenosine-5'-diphosphate: Y74 (= Y81), T151 (= T158), R192 (= R199), Y228 (= Y235), H229 (= H236), F272 (= F279), Q316 (= Q323), N352 (= N359), E356 (= E363), L360 (= L367), P361 (= P368)
- binding cobalamin: F102 (= F109), L104 (= L111), H107 (= H114), A124 (= A131), V191 (= V198), R192 (= R199), H229 (= H236), E232 (= E239), G319 (= G326), W320 (≠ V327), E356 (= E363), G359 (≠ A366), L360 (= L367), G590 (= G597), H591 (= H598), D592 (= D599), R593 (= R600), G594 (= G601), I598 (= I605), S636 (= S643), L638 (= L645), A640 (= A647), G666 (= G673), G667 (= G674), V668 (= V675), F686 (≠ Y693), G687 (= G694), T690 (= T697)
2xiqA Crystal structure of human methylmalonyl-coa mutase in complex with adenosylcobalamin and malonyl-coa (see paper)
65% identity, 99% coverage: 6:712/716 of query aligns to 2:706/714 of 2xiqA
- active site: Y75 (= Y81), Y229 (= Y235), H230 (= H236), K586 (= K592), D590 (= D596), H592 (= H598)
- binding cobalamin: Y75 (= Y81), L105 (= L111), H108 (= H114), A125 (= A131), R193 (= R199), E233 (= E239), G320 (= G326), W321 (≠ V327), E357 (= E363), G360 (≠ A366), L361 (= L367), G591 (= G597), H592 (= H598), D593 (= D599), R594 (= R600), G595 (= G601), I599 (= I605), G635 (= G641), S637 (= S643), L639 (= L645), A641 (= A647), G667 (= G673), G668 (= G674), F687 (≠ Y693), G688 (= G694), T691 (= T697)
- binding malonyl-coenzyme a: Y61 (≠ R67), T63 (= T69), M64 (= M70), R68 (= R74), T71 (= T77), R73 (= R79), Y75 (= Y81), S150 (= S156), T152 (= T158), T181 (= T187), R193 (= R199), K220 (= K226), H230 (= H236), R269 (= R275), S271 (= S277), F273 (= F279), R313 (= R319), A314 (≠ T320), H315 (= H321), Q317 (= Q323), Q348 (= Q354)
P22033 Methylmalonyl-CoA mutase, mitochondrial; MCM; Methylmalonyl-CoA isomerase; EC 5.4.99.2 from Homo sapiens (Human) (see 28 papers)
65% identity, 99% coverage: 6:712/716 of query aligns to 37:741/750 of P22033
- Q50 (≠ E19) binding malonyl-CoA
- I69 (= I38) to V: in MMAM; likely benign; dbSNP:rs115923556
- P86 (= P57) to L: in MMAM; mut0 and mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769348060
- G87 (= G58) to E: in MMAM; mut0; dbSNP:rs1554160986
- R93 (= R64) to H: in MMAM; mut0; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918251
- G94 (= G65) to R: in MMAM; mut0; dbSNP:rs727504022; to V: in MMAM; mut- and mut0; dbSNP:rs535411418
- P95 (= P66) to R: in MMAM; mut0; dbSNP:rs190834116
- YPTM 96:99 (≠ RATM 67:70) binding malonyl-CoA
- Y100 (= Y71) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309735
- W105 (= W76) to R: in MMAM; mut0; dbSNP:rs121918249
- TIRQY 106:110 (≠ TVRQY 77:81) binding malonyl-CoA
- R108 (= R79) to C: in MMAM; mut0; dbSNP:rs121918257; to G: in MMAM; mut-; dbSNP:rs121918257; to H: in MMAM; mut0; dbSNP:rs483352778
- Q109 (= Q80) to R: in MMAM; mut0; dbSNP:rs1461110052
- G133 (= G104) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253828
- A137 (= A108) to V: in MMAM; mut0; dbSNP:rs941483851
- D139 (= D110) to N: in MMAM; uncertain significance; dbSNP:rs879253829
- L140 (= L111) to P: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
- A141 (= A112) to T: in MMAM; decreased protein expression; dbSNP:rs1554160730
- H143 (= H114) to Y: in MMAM; mut0
- G145 (= G116) to S: in MMAM; mut0; dbSNP:rs1237080100
- S148 (= S119) to L: in MMAM; mut0; dbSNP:rs1300547552
- D156 (= D127) to N: in MMAM; mut-
- G158 (= G129) to V: in MMAM; mut0
- G161 (= G132) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs2127420039; to V: in MMAM; decreased protein expression
- F174 (= F145) to S: in MMAM; mut0; dbSNP:rs864309733
- M186 (= M157) to V: in MMAM; mut-; dbSNP:rs148331800
- T187 (= T158) to S: in MMAM; mut0; dbSNP:rs879253830
- N189 (= N160) to I: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs200908035; to K: in MMAM; mut-; dbSNP:rs1561959114
- A191 (= A162) to E: in MMAM; mut- and mut0; affects proper folding; reduced protein level; decreased methylmalonyl-CoA mutase activity; dbSNP:rs760782399
- A197 (= A168) to E: in MMAM; mut0
- G203 (≠ A174) to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs778702777
- E205 (= E176) natural variant: Missing (in MMAM; mut0; dbSNP:rs879253831)
- G215 (= G186) to C: in MMAM; mut- and mut0; dbSNP:rs121918258; to S: in MMAM; mut0; dbSNP:rs121918258
- TIQ 216:218 (≠ TLQ 187:189) binding malonyl-CoA
- Q218 (= Q189) to H: in MMAM; mut0 and mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs1446389693
- N219 (= N190) to Y: in MMAM; mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs121918256
- R228 (= R199) binding malonyl-CoA; to Q: in MMAM; mut0; dbSNP:rs770810987
- T230 (= T201) to I: in MMAM; mut-; dbSNP:rs879253833; to R: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253833
- Y231 (= Y202) to N: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309736
- K255 (= K226) binding malonyl-CoA
- S262 (= S233) to N: in MMAM; mut0; dbSNP:rs1767683356
- H265 (= H236) binding malonyl-CoA; to Y: in MMAM; mut-
- E276 (= E247) to D: in MMAM; uncertain significance; mut-; dbSNP:rs12175488
- L281 (≠ I252) to S: in MMAM; mut0; dbSNP:rs796052007
- G284 (= G255) to E: in MMAM; mut0; dbSNP:rs879253835; to R: in MMAM; mut0; dbSNP:rs761477436
- S288 (≠ V259) to P: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1179778233
- G291 (≠ A262) to E: in MMAM; mut0
- Q293 (≠ A264) to P: in MMAM; mut0; dbSNP:rs138374956
- RLS 304:306 (= RLS 275:277) binding malonyl-CoA
- L305 (= L276) to S: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554160246
- S306 (= S277) to F: in MMAM; mut0; dbSNP:rs1085307929
- W309 (≠ F280) to G: in MMAM; decreased protein expression; dbSNP:rs2127418704
- G312 (= G283) to V: in MMAM; mut0; dbSNP:rs864309734
- Y316 (≠ F287) to C: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; no decreased affinity for adenosylcob(III)alamin; dbSNP:rs781474200
- A324 (= A295) to T: in MMAM; mut-; dbSNP:rs780387525
- R326 (= R297) to K: in MMAM; uncertain significance; dbSNP:rs758577372
- L328 (= L299) to F: in MMAM; mut0; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; dbSNP:rs796052002; to P: in MMAM; mut0; dbSNP:rs965316043
- S344 (= S315) to F: in MMAM; mut-; affects proper folding; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin
- L346 (≠ M317) natural variant: Missing (in MMAM; mut0)
- L347 (= L318) to R: in MMAM; mut0; dbSNP:rs1026703654
- H350 (= H321) to Y: in MMAM; mut0; dbSNP:rs1407914109
- L358 (= L329) to P: in MMAM; mut0; dbSNP:rs1767612147
- N366 (= N337) to S: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs864309737
- R369 (= R340) to C: in MMAM; mut0; dbSNP:rs772552898; to H: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; alters thermodynamic stability; dbSNP:rs564069299
- T370 (= T341) to P: in MMAM; mut0; dbSNP:rs368790885
- A377 (= A348) to E: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs121918250
- Q383 (= Q354) to H: in MMAM; mut0; to P: in MMAM; mut0
- H386 (= H357) to N: in MMAM; mut0; dbSNP:rs1554159937; to R: in MMAM; mut0; dbSNP:rs866933356
- T387 (= T358) to I: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs1400022403
- N388 (= N359) to H: in MMAM; mut0; dbSNP:rs766010704; to K: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253840
- S389 (≠ A360) natural variant: Missing (in MMAM; mut0)
- I412 (= I383) natural variant: Missing (in MMAM; mut0)
- P424 (= P395) to L: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253842
- G426 (≠ A397) to E: in MMAM; mut-; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs533755473; to R: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs769922244
- G427 (= G398) to D: in MMAM; mut0; dbSNP:rs753288303
- G454 (= G425) to E: in MMAM; mut0
- A499 (≠ E470) to T: in dbSNP:rs2229385
- I505 (= I476) to T: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity
- Q514 (= Q485) to E: in MMAM; uncertain significance; to K: in MMAM; decreased protein expression; dbSNP:rs2127416800
- L518 (= L489) to P: in MMAM; mut0; dbSNP:rs864309738
- R532 (≠ A503) to H: in dbSNP:rs1141321
- A535 (≠ D506) to P: in MMAM; mut0; dbSNP:rs760183775
- A552 (= A523) to V: in MMAM; uncertain significance; dbSNP:rs879253845
- C560 (≠ A531) to Y: in MMAM; mut0; dbSNP:rs1238333040
- T566 (≠ S537) to R: in MMAM; mut0; dbSNP:rs1767288356
- F573 (≠ Y544) to S: in MMAM; mut-; affects proper folding; no effect on protein abundance; no effect on methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs775593146
- Y587 (= Y558) to C: in MMAM; mut-
- I597 (≠ F568) to R: in MMAM; no changed in protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1554158951
- P615 (= P586) to L: in MMAM; mut0; affects proper folding; reduced strongly protein level; dbSNP:rs1554158777; to R: in MMAM; mut0; dbSNP:rs1554158777; to T: in MMAM; mut0; affects proper folding; reduced strongly protein level; loss of methylmalonyl-CoA mutase activity; dbSNP:rs1302409621
- R616 (= R587) to C: in MMAM; mut0; dbSNP:rs765284825
- L617 (≠ M588) to R: in MMAM; mut0; dbSNP:rs1554158775
- K621 (= K592) to N: in MMAM; mut0
- G623 (= G594) to R: in MMAM; mut0; dbSNP:rs121918254
- Q624 (= Q595) to R: in MMAM; no effect on protein abundance; dbSNP:rs768521956
- D625 (= D596) to G: in MMAM; mut0; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs879253847; to V: in MMAM; mut0; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity
- G626 (= G597) to C: in MMAM; mut-; dbSNP:rs982110849
- H627 (= H598) binding axial binding residue; to R: in MMAM; mut0; dbSNP:rs372486357
- G630 (= G601) to E: in MMAM; mut0; dbSNP:rs143023066
- V633 (= V604) to G: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; does not alter thermodynamic stability; dbSNP:rs200055428
- G637 (≠ A608) to E: in MMAM; mut-; to R: in MMAM; mut0; dbSNP:rs781501004
- F638 (≠ Y609) to I: in MMAM; mut0
- D640 (= D611) to Y: in MMAM; mut0; dbSNP:rs865815395
- G642 (= G613) to R: in MMAM; mut-; dbSNP:rs747897332
- G648 (= G619) to D: in MMAM; mut-; no effect on protein abundance; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs766721811
- V669 (= V640) to E: in MMAM; mut0; dbSNP:rs1360470463
- I671 (≠ V642) to V: in dbSNP:rs8589
- L674 (= L645) to F: in MMAM; decreased protein abundance; decreased methylmalonyl-CoA mutase activity; dbSNP:rs1164271240
- H678 (= H649) to R: in MMAM; mut-; dbSNP:rs147094927
- E684 (≠ A655) natural variant: E -> EL (in MMAM; mut-)
- L685 (= L656) to R: in MMAM; mut-; dbSNP:rs864309739
- R694 (≠ A665) to L: in MMAM; mut-; decreased protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs756225782; to W: in MMAM; mut- and mut0; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs777758903
- M700 (≠ V671) to K: in MMAM; mut-; no effect on protein abundance; loss of methylmalonyl-CoA mutase activity; decreased affinity for adenosylcob(III)alamin; alters thermodynamic stability; dbSNP:rs140600746
- G703 (= G674) to R: in MMAM; mut0; dbSNP:rs121918255
- G717 (= G688) to V: in MMAM; mut-; no effect on protein abundance; interferes with the binding of the cofactor to the apoenzyme; decreased methylmalonyl-CoA mutase activity; strong decreased affinity for adenosylcob(III)alamin; decreased thermodynamic stability; dbSNP:rs121918252
- G723 (= G694) to D: in MMAM; decreased protein expression; decreased methylmalonyl-CoA mutase activity; dbSNP:rs755077681
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
- 7:750 natural variant: Missing (in MMAM; mut-)
- 152:750 natural variant: Missing (in MMAM; mut0)
- 228:750 natural variant: Missing (in MMAM; mut0)
8gjuJ Crystal structure of human methylmalonyl-coa mutase (mmut) in complex with methylmalonic acidemia type a protein (mmaa), coenzyme a, and gdp (see paper)
64% identity, 99% coverage: 6:712/716 of query aligns to 2:683/689 of 8gjuJ