SitesBLAST
Comparing WP_029133164.1 NCBI__GCF_000428045.1:WP_029133164.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P0A9G6 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Escherichia coli (strain K12) (see 3 papers)
76% identity, 99% coverage: 2:434/436 of query aligns to 3:434/434 of P0A9G6
- SGW 91:93 (= SGW 91:93) binding substrate
- D157 (= D157) binding Mg(2+)
- C195 (= C195) active site, Proton acceptor; mutation to A: Large decrease in activity.; mutation to S: Large decrease in activity.
- A219 (= A219) mutation to C: Isocitrate lyase activity is reduced compared to the wild-type.
- R232 (= R232) binding substrate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1igwC Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
75% identity, 95% coverage: 2:417/436 of query aligns to 2:416/416 of 1igwC
- active site: Y88 (= Y89), D107 (= D108), D156 (= D157), E158 (= E159), H183 (= H184), E185 (= E186), C194 (= C195), R231 (= R232), E288 (= E289), K311 (= K312), S318 (= S319), S320 (= S321)
- binding pyruvic acid: S90 (= S91), G91 (= G92), W92 (= W93), D156 (= D157), R231 (= R232), T350 (= T351)
1igwA Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
71% identity, 95% coverage: 2:417/436 of query aligns to 2:396/396 of 1igwA
- active site: Y88 (= Y89), D107 (= D108), D156 (= D157), E158 (= E159), H183 (= H184), E185 (= E186), C194 (= C195), R227 (= R232), E284 (= E289), K307 (= K312)
- binding pyruvic acid: S90 (= S91), W92 (= W93), D156 (= D157), R227 (= R232), T330 (= T351)
6lrtA Crystal structure of isocitrate lyase (caur_3889) from chloroflexus aurantiacus in complex with isocitrate and manganese ion
64% identity, 100% coverage: 1:434/436 of query aligns to 1:423/423 of 6lrtA
7cmyC Isocitrate lyase from bacillus cereus atcc 14579 in complex with magnessium ion, glyoxylate, and succinate
61% identity, 99% coverage: 3:434/436 of query aligns to 1:417/417 of 7cmyC
7rb1A Isocitrate lyase-1 from mycobacterium tuberculosis covalently modified by 5-descarboxy-5-nitro-d-isocitric acid (see paper)
62% identity, 98% coverage: 8:434/436 of query aligns to 11:427/427 of 7rb1A
- binding dihydroxyacetic acid: Y89 (= Y89), S91 (= S91), W93 (= W93), D153 (= D157), R228 (= R232), T347 (= T351)
- binding (3E)-3-(hydroxyimino)propanoic acid: C191 (= C195), G192 (= G196), H193 (= H197), R228 (= R232), S315 (= S319), S317 (= S321), T347 (= T351)
- binding magnesium ion: A276 (= A280), A279 (= A283), Q308 (≠ K312)
6wsiA Intact cis-2,3-epoxysuccinic acid bound to isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
62% identity, 98% coverage: 8:434/436 of query aligns to 11:427/427 of 6wsiA
- active site: Y89 (= Y89), D108 (= D108), D153 (= D157), E155 (= E159), H180 (= H184), E182 (= E186), C191 (= C195), H193 (= H197), R228 (= R232), E285 (= E289), Q308 (≠ K312), S315 (= S319), S317 (= S321)
- binding magnesium ion: A276 (= A280), A279 (= A283), Q308 (≠ K312)
- binding (2R,3S)-oxirane-2,3-dicarboxylic acid: C191 (= C195), G192 (= G196), H193 (= H197), R228 (= R232), E285 (= E289), N313 (= N317), S315 (= S319), S317 (= S321), T347 (= T351)
6vb9A Covalent adduct of cis-2,3-epoxysuccinic acid with isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
62% identity, 98% coverage: 8:434/436 of query aligns to 11:427/427 of 6vb9A
- active site: Y89 (= Y89), D108 (= D108), D153 (= D157), E155 (= E159), H180 (= H184), E182 (= E186), C191 (= C195), H193 (= H197), R228 (= R232), E285 (= E289), Q308 (≠ K312), S315 (= S319), S317 (= S321)
- binding magnesium ion: A276 (= A280), A279 (= A283), Q308 (≠ K312)
- binding oxalic acid: Y89 (= Y89), S91 (= S91), G92 (= G92), W93 (= W93), D153 (= D157), C191 (= C195), R228 (= R232), W283 (= W287), T347 (= T351)
5dqlA Crystal structure of 2-vinyl glyoxylate modified isocitrate lyase from mycobacterium tuberculosis (see paper)
62% identity, 98% coverage: 8:434/436 of query aligns to 11:427/427 of 5dqlA
- active site: Y89 (= Y89), D108 (= D108), D153 (= D157), E155 (= E159), H180 (= H184), E182 (= E186), C191 (= C195), H193 (= H197), R228 (= R232), E285 (= E289), Q308 (≠ K312), S315 (= S319), S317 (= S321)
- binding magnesium ion: A276 (= A280), A279 (= A283), Q308 (≠ K312)
- binding 4-hydroxy-2-oxobutanoic acid: W93 (= W93), D108 (= D108), C191 (= C195), H193 (= H197), S315 (= S319), S317 (= S321), T347 (= T351), L348 (= L352)
6c4aA Crystal structure of 3-nitropropionate modified isocitrate lyase from mycobacterium tuberculosis with pyruvate (see paper)
62% identity, 98% coverage: 8:434/436 of query aligns to 12:428/428 of 6c4aA
- active site: Y90 (= Y89), D109 (= D108), D154 (= D157), E156 (= E159), H181 (= H184), E183 (= E186), C192 (= C195), H194 (= H197), R229 (= R232), E286 (= E289), Q309 (≠ K312), S316 (= S319), S318 (= S321)
- binding 3-nitropropanoic acid: Y357 (= Y360), S358 (≠ N361), R380 (≠ P385)
- binding magnesium ion: A277 (= A280), A280 (= A283), Q309 (≠ K312)
- binding pyruvic acid: Y90 (= Y89), S92 (= S91), G93 (= G92), W94 (= W93), D154 (= D157), C192 (= C195), R229 (= R232), W284 (= W287), T348 (= T351)
P9WKK7 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
62% identity, 98% coverage: 8:434/436 of query aligns to 11:427/428 of P9WKK7