SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_029329000.1 NCBI__GCF_000224785.1:WP_029329000.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
78% identity, 99% coverage: 7:445/445 of query aligns to 6:444/444 of P12425

query
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P12425

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G59 (= G60) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (= R63) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E133) binding Mg(2+)
E134 (= E135) binding Mg(2+)
E189 (= E190) binding Mg(2+)
V190 (= V191) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E197) binding Mg(2+)
G241 (= G242) binding L-glutamate
H245 (= H246) binding Mg(2+)
G302 (= G303) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (= E305) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P307) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E334) binding Mg(2+)
E424 (= E425) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
78% identity, 99% coverage: 7:445/445 of query aligns to 5:443/443 of 4lnkA

query
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4lnkA

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active site: D52 (= D54), E131 (= E133), E133 (= E135), E188 (= E190), E195 (= E197), H244 (= H246), R315 (= R317), E332 (= E334), R334 (= R336)
binding adenosine-5'-diphosphate: K43 (= K45), M50 (= M52), F198 (= F200), Y200 (= Y202), N246 (= N248), S248 (= S250), S324 (= S326), S328 (= S330), R330 (= R332)
binding glutamic acid: E133 (= E135), E188 (= E190), V189 (= V191), N239 (= N241), G240 (= G242), G242 (= G244), E303 (= E305)
binding magnesium ion: E131 (= E133), E188 (= E190), E195 (= E197), H244 (= H246), E332 (= E334)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
78% identity, 99% coverage: 7:445/445 of query aligns to 5:443/443 of 4lniA

query
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4lniA

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L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

V

A

A

W

W

S

S

G

A

L

Q

N

N

R
|
R

S

S

P

P

L

L

V

I

R
|
R

V

I

P

P

S

A

S

S

R

R

G

G

L

I

S

S

T

T

R
|
R

I

V

E
|
E

A

V

R
|
R

S

S

V

V

D

D

P

P

A

A

N

N

P

P

Y

Y

M

L

T

A

L

L

A

S

V

V

L

L

S

A

A

A

G

G

L

L

D

D

G

G

I

I

E

K

N

N

K

K

L

L

T

E

P

A

P

P

E

A

P

P

V

I

D

D

Q

R

N

N

I

I

Y

Y

E

V

M

M

D

S

K

K

A

E

E

E

R

R

E

M

E

E

H

N

G

G

I

I

K

V

S

D

L

L

P

P

A

A

T

T

L

L

M

A

D

E

A

A

L

L

E

E

L

F

D

K

N

S

D

N

P

E

V

V

I

M

T

V

E

K

A

A

L

L

G

G

N

E

H

H

L

L

Y

F

E

E

H

H

F

F

V

I

E

E

A

A

K

K

Q

E

I

I

E

E

W

W

D

D

M

M

F

F

R

R

T

T

A

Q

V

V

H

H

P

P

W

W

E

E

R

R

E

E

Q

Q

Y

Y

L

M

T

S

S

Q

Y

Y

active site: D52 (= D54), E131 (= E133), E133 (= E135), E188 (= E190), E195 (= E197), H244 (= H246), R315 (= R317), E332 (= E334), R334 (= R336)
binding adenosine-5'-diphosphate: E131 (= E133), E183 (= E185), D197 (= D199), Y200 (= Y202), N246 (= N248), S248 (= S250), R320 (= R322), R330 (= R332)
binding magnesium ion: E131 (= E133), E131 (= E133), E133 (= E135), E188 (= E190), E195 (= E197), E195 (= E197), H244 (= H246), E332 (= E334)
binding l-methionine-s-sulfoximine phosphate: E133 (= E135), E188 (= E190), H244 (= H246), R297 (= R299), E303 (= E305), R315 (= R317), R334 (= R336)

4s0rD Structure of gs-tnra complex (see paper)
78% identity, 99% coverage: 7:445/445 of query aligns to 9:447/447 of 4s0rD

query
sites
4s0rD

K

R

Q

E

D

D

I

I

Y

E

K

K

M

L

I

V

D

K

E

E

S

N

V

V

R

K

F

Y

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

M

I

L

L

G

G

T

T

I

I

K

K

N

N

V

V

E

E

I

I

P

P

Y

V

S

S

Q

Q

L

L

E

G

K

K

A

A

F

L

D

D

N

N

K

K

M

V

M

M

F

F

D
|
D

G

G

S

S

I

I

E

E

G

G

F
|
F

V
|
V

R
|
R

I
|
I

D

E

E

E

S

S

D

D

M

M

Y

Y

L

L

Y

Y

P

P

D

D

L

L

D

N

T

T

F

F

V

V

V

I

F

F

P

P

W

W

T

T

A

A

E

E

K

K

G

G

K

K

V

V

A

A

R

R

F

F

I

I

C

C

D

D

I

I

Y

Y

N

N

P

P

D

D

G

G

T

T

P

P

F

F

E

E

G

G

C

D

P

P

R

R

Y

N

N

N

L

L

K

K

R

R

N

I

L

L

K

K

R

E

M

M

E

E

E

D

L

L

G

G

Y

F

S

S

A

D

F

F

N

N

I

L

G

G

T

P

E
|
E

P

P

E
|
E

F

F

L

L

F

F

K

K

L

L

D

D

E

E

K

K

G

G

D

E

P

P

M

T

M

L

E

E

L

L

N

N

D

D

R

K

G

G

Y

Y

F

F

D
|
D

L

L

A

A

P

P

T

T

D

D

L

L

G

G

E

E

N

N

C

C

R

R

D

D

I

I

V

V

L

L

E

E

L

L

E

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

G

A

A

V

V

K

R

H

S

A

C

D

D

I

I

Q

Q

T

T

F

F

K

K

L

L

V

V

K

K

T

T

I

I

A

A

R

R

K

K

H

H

N

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

F

F

G
|
G

V
|
V

N
|
N

G

G

S

S

G

G

M

M

H
|
H

V

C

N

N

M

L

S

S

L

L

F

F

K

K

D

N

G

G

R

V

N

N

V

A

F

F

Y

F

D

D

E

E

K

N

G

A

D

D

M

L

Q

Q

V

L

S

S

T

E

T

T

L

A

K

K

Q

H

F

F

T

I

A

A

G

G

I

I

I

V

K

K

H

H

A

A

T

T

N

S

F

F

T

T

A

A

V

V

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

C

C

Y

Y

V

V

A

A

W

W

S

S

G

A

L

Q

N

N

R
|
R

S

S

P

P

L

L

V

I

R

R

V

I

P

P

S

A

S

S

R

R

G

G

L

I

S

S

T

T

R

R

I

V

E
|
E

A

V

R
|
R

S

S

V

V

D

D

P

P

A

A

N

N

P

P

Y

Y

M

L

T

A

L

L

A

S

V

V

L

L

S

A

A

A

G

G

L

L

D

D

G

G

I

I

E

K

N

N

K

K

L

L

T

E

P

A

P

P

E

A

P

P

V

I

D

D

Q

R

N

N

I

I

Y
|
Y

E

V

M

M

D

S

K

K

A

E

E

E

R

R

E

M

E

E

H

N

G

G

I

I

K

V

S

D

L

L

P

P

A

A

T

T

L

L

M

A

D

E

A

A

L

L

E

E

L

F

D

K

N

S

D

N

P

E

V

V

I

M

T

V

E

K

A

A

L

L

G

G

N

E

H

H

L

L

Y

F

E

E

H
|
H

F

F

V

I

E

E

A

A

K

K

Q

E

I
|
I

E

E

W

W

D

D

M
|
M

F

F

R

R

T

T

A

Q

V

V

H

H

P

P

W

W

E

E

R

R

E

E

Q

Q

Y

Y

L

M

T

S

S

Q

Y

Y

active site: D56 (= D54), E135 (= E133), E137 (= E135), E192 (= E190), E199 (= E197), H248 (= H246), R319 (= R317), E336 (= E334), R338 (= R336)
binding glutamine: E137 (= E135), E192 (= E190), R301 (= R299), E307 (= E305)
binding magnesium ion: I66 (= I64), E135 (= E133), E135 (= E133), E199 (= E197), H248 (= H246), H248 (= H246), E336 (= E334), H419 (= H417)
binding : F63 (= F61), V64 (= V62), R65 (= R63), I66 (= I64), D161 (= D159), G241 (= G239), V242 (= V240), N243 (= N241), G305 (= G303), Y306 (= Y304), Y376 (= Y374), I426 (= I424), M430 (= M428)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
75% identity, 99% coverage: 7:445/445 of query aligns to 5:443/443 of 7tf9S

query
sites
7tf9S

K

K

Q

E

D

D

I

I

Y

F

K

R

M

F

I

A

D

D

E

E

E

Q

S

N

V

V

R

K

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

M

I

L

L

G

G

T

I

I

I

K

K

N

N

V

V

E

E

I

I

P

P

Y

V

S

S

Q

Q

L

L

E

K

K

K

A

A

F

L

D

D

N

N

K

K

M

I

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
|
F

V
|
V

R

R

I

I

D

E

E

E

S

S

D

D

M

M

Y

Y

L

L

Y

F

P

P

D

D

L

L

D

D

T

T

F

W

V

V

F

F

P

P

W

W

T

T

A

A

E

E

K

K

G

G

K

K

V

V

A

A

R

R

F

M

I

I

C

C

D

D

I

I

Y

Y

N

N

P

P

D

D

G

M

T

T

P

P

F

F

E

A

G

G

C

D

P

P

R

R

Y

A

N

N

L

L

K

K

R

R

N

V

L

L

K

K

R

E

M

M

E

E

L

L

G

G

Y

F

S

T

A

E

F

F

N

N

I

L

G

G

T

P

E
|
E

P

P

E
|
E

F

F

L

L

F

F

K

K

L

L

D

D

E

E

K

N

G

R

D

R

P

P

M

T

M

L

E

E

L

L

N

N

D

D

R

S

G

G

Y
|
Y

F

F

D

D

L

L

A

A

P

P

T

T

D

D

L

L

G

G

E

E

N

N

C

C

R

R

D

D

I

I

V

V

L

L

E

E

L

L

E

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

E

D

D

A

A

V

I

K

T

H

A

A

C

D

D

D

S

I

I

Q

Q

T

T

F

F

K

K

L

L

V

V

K

K

T

T

I

I

A

A

R

R

K

K

H

H

N

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

F

F

G

G

V

V

N

N

G
|
G

S

S

G
|
G

M

M

H
|
H

V

F

N

N

M

M

S

S

L

L

F

F

K

N

D

E

G

K

R

G

N

N

V

A

F

F

Y

F

D

D

E

E

K

S

G

G

D

E

M

L

Q

E

V

L

S

S

T

Q

T

T

L

A

K

Y

Q

H

F

F

T

L

A

A

G

G

I

M

I

L

K

K

H

H

A

A

T

R

N

G

F

Y

T

T

A

A

V

V

T

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

I

A

A

W

W

S

S

G

G

L

K

N

N

R

R

S

S

P

P

L

L

V

V

R

R

V

V

P

P

S

S

R

R

G

G

L

L

S

S

T

T

R

R

I

L

E
|
E

A

L

R

R

S

S

V

V

D

D

P

P

A

S

A

A

N

N

P

P

Y

Y

M

L

T

A

L

M

A

A

V

V

L

L

S

K

A

A

G

G

L

L

D

S

G

G

I

I

E

K

N

D

K

E

L

L

T

T

P

P

E

A

P

P

V

V

D

D

Q

R

N

N

I

I

Y

Y

E

G

M

M

D

N

K

E

A

E

E

E

R

R

E

E

E

A

H

T

G

G

I

I

K

Y

S

D

L

L

P

P

A

E

T

S

L

L

M

G

D

H

A

A

L

L

E

I

E

E

L

L

D

E

N

K

D

N

P

E

V

I

I

I

T

K

E

D

A

G

L

L

G

G

N

E

H

H

L

I

Y

F

E

E

H

H

F

F

V

I

E
|
E

A

A

K

K

Q

T

I
|
I

E

E

W

C

D

D

M
|
M

F

F

R

R

T

T

A

A

V

V

H

H

P

P

W

W

E

E

R

R

E

E

Q

Q

Y

Y

L

L

T

E

S

I

Y

Y

binding glutamine: E133 (= E135), Y155 (= Y157), E188 (= E190), G240 (= G242), G242 (= G244), R297 (= R299), E303 (= E305)
binding magnesium ion: E131 (= E133), E133 (= E135), E188 (= E190), E195 (= E197), H244 (= H246), E332 (= E334)
binding : F59 (= F61), V60 (= V62), E418 (= E420), I422 (= I424), M426 (= M428)

7tenA Glutamine synthetase (see paper)
75% identity, 99% coverage: 7:445/445 of query aligns to 4:442/442 of 7tenA

query
sites
7tenA

K

K

Q

E

D

D

I

I

Y

F

K

R

M

F

I

A

D

D

E

E

E

Q

S

N

V

V

R

K

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

M

I

L

L

G

G

T

I

I

I

K

K

N

N

V

V

E

E

I

I

P

P

Y

V

S

S

Q

Q

L

L

E

K

K

K

A

A

F

L

D

D

N

N

K

K

M

I

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

D

E

E

E

S

S

D

D

M

M

Y

Y

L

L

Y

F

P

P

D

D

L

L

D

D

T

T

F

W

V

V

F

F

P

P

W

W

T

T

A

A

E

E

K

K

G

G

K

K

V

V

A

A

R

R

F

M

I

I

C

C

D

D

I

I

Y

Y

N

N

P

P

D

D

G

M

T

T

P

P

F

F

E

A

G

G

C

D

P

P

R

R

Y

A

N

N

L

L

K

K

R

R

N

V

L

L

K

K

R

E

M

M

E

E

L

L

G

G

Y

F

S

T

A

E

F

F

N

N

I

L

G
|
G

T

P

E
|
E

P

P

E
|
E

F

F

L

L

F

F

K

K

L

L

D

D

E

E

K

N

G

R

D

R

P

P

M

T

M

L

E

E

L

L

N

N

D

D

R

S

G

G

Y

Y

F

F

D

D

L

L

A

A

P

P

T

T

D

D

L

L

G

G

E

E

N

N

C

C

R

R

D

D

I

I

V

V

L

L

E

E

L

L

E

E

M

M

G

G

F

F

E

E

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K
|
K

Y
|
Y

S

E

D

D

A

A

V

I

K

T

H

A

A

C

D

D

D

S

I

I

Q

Q

T

T

F

F

K

K

L

L

V

V

K

K

T

T

I

I

A

A

R

R

K

K

H

H

N

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

F

F

G

G

V

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

V

F

N
|
N

M

M

S
|
S

L

L

F

F

K

N

D

E

G

K

R

G

N

N

V

A

F

F

Y

F

D

D

E

E

K

S

G

G

D

E

M

L

Q

E

V

L

S

S

T

Q

T

T

L

A

K

Y

Q

H

F

F

T

L

A

A

G

G

I

M

I

L

K

K

H

H

A

A

T

R

N

G

F

Y

T

T

A

A

V

V

T

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

I

A

A

W

W

S

S

G

G

L

K

N

N

R
|
R

S

S

P

P

L

L

V

V

R
|
R

V

V

P

P

S

S

R

R

G

G

L

L

S
|
S

T

T

R
|
R

I

L

E

E

A

L

R
|
R

S

S

V

V

D

D

P

P

A

S

A

A

N

N

P

P

Y

Y

M

L

T

A

L

M

A

A

V

V

L

L

S

K

A

A

G

G

L

L

D

S

G

G

I

I

E

K

N

D

K

E

L

L

T

T

P

P

E

A

P

P

V

V

D

D

Q

R

N

N

I

I

Y

Y

E

G

M

M

D

N

K

E

A

E

E

E

R

R

E

E

E

A

H

T

G

G

I

I

K

Y

S

D

L

L

P

P

A

E

T

S

L

L

M

G

D

H

A

A

L

L

E

I

E

E

L

L

D

E

N

K

D

N

P

E

V

I

I

I

T

K

E

D

A

G

L

L

G

G

N

E

H

H

L

I

Y

F

E

E

H

H

F

F

V

I

E

E

A

A

K

K

Q

T

I

I

E

E

W

C

D

D

M

M

F

F

R

R

T

T

A

A

V

V

H

H

P

P

W

W

E

E

R

R

E

E

Q

Q

Y

Y

L

L

T

E

S

I

Y

Y

binding adenosine-5'-diphosphate: G128 (= G131), E130 (= E133), E182 (= E185), D196 (= D199), F197 (= F200), K198 (= K201), Y199 (= Y202), N245 (= N248), S247 (= S250), R319 (= R322), S327 (= S330), R329 (= R332)
binding l-methionine-s-sulfoximine phosphate: E130 (= E133), E132 (= E135), E187 (= E190), E194 (= E197), N238 (= N241), G239 (= G242), H243 (= H246), R296 (= R299), E302 (= E305), R314 (= R317), R333 (= R336)

7tdvC Glutamine synthetase (see paper)
74% identity, 99% coverage: 7:445/445 of query aligns to 5:443/443 of 7tdvC

query
sites
7tdvC

K

K

Q

E

D

D

I

I

Y

R

K

K

M

F

I

V

D

E

E

E

S

N

V

V

R

R

F

Y

I

L

R

R

L

L

Q

Q

F

F

T

T

D

D

M

I

L

L

G

G

T

T

I

I

K

K

N

N

V

V

E

E

I

V

P

P

Y

V

S

S

Q

Q

L

L

E

E

K

K

A

V

F

L

D

D

N

N

K

E

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

F

V

V

R

R

I

I

D

E

E

E

S

S

D

D

M

M

Y

Y

L

L

Y

H

P

P

D

D

L

L

D

D

T

T

F

W

V

V

V

I

F

F

P

P

W

W

T

T

A

A

E

G

K

Q

G

G

K

K

V

V

A

A

R

R

F

L

I

I

C

C

D

D

I

V

Y

Y

N

K

P

T

D

D

G

G

T

T

P

P

F

F

E

E

G

G

C

D

P

P

R

R

Y

A

N

N

L

L

K

K

R

R

N

V

L

L

K

K

R

E

M

M

E

E

E

D

L

L

G

G

Y

F

S

T

A

D

F

F

N

N

I

L

G
|
G

T

P

E
|
E

P

P

E
|
E

F

F

L

L

F

F

K

K

L

L

D

D

E

E

K

K

G

G

D

E

P

P

M

T

M

L

E

E

L

L

N

N

D

D

R

D

G

G

Y

Y

F

F

D

D

L

L

A

A

P

P

T

T

D

D

L

L

G

G

E

E

N

N

C

C

R

R

D

D

I

I

V

V

L

L

E

E

L

L

E

E

E

D

M

M

G

G

F

F

E

D

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F
|
F

K
|
K

Y
|
Y

S

A

D

D

A

A

V

V

K

T

H

A

A

C

D

D

D

N

I

I

Q

Q

T

T

F

F

K

K

L

L

V

V

K

K

T

T

I

I

A

A

R

R

K

K

H

H

N

N

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

F

F

G

G

V

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

V

F

N
|
N

M
x
V

S
|
S

L

L

F

F

K

K

D

G

G

K

R

E

N

N

V

A

F

F

Y

F

D

D

E

P

K

N

G

T

D

E

M

M

Q

G

V

L

S

T

T

E

T

T

L

A

K

Y

Q

Q

F

F

T

T

A

A

G

G

I

V

I

L

K

K

H

N

A

A

T

R

N

G

F

F

T

T

A

A

V

V

T

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

I

A

A

W

W

S

S

G

G

L

K

N

N

R
|
R

S

S

P

P

L

L

V

I

R
|
R

V

V

P

P

S

S

R

R

G

G

L

L

S
|
S

T

T

R
|
R

I

I

E
|
E

A

V

R

R

S

S

V

V

D

D

P

P

A

A

N

N

P

P

Y

Y

M

M

T

A

L

L

A

A

V

A

L

I

L

L

S

E

A

A

G

G

L

L

D

D

G

G

I

I

E

K

N

N

K

K

L

L

T

K

P

V

P

P

E

E

P

P

V

V

D

N

Q

Q

N

N

I

I

Y

Y

E

E

M

M

D

N

K

R

A

E

E

E

R

R

E

E

E

A

H

V

G

G

I

I

K

Q

S

D

L

L

P

P

A

S

T

T

L

L

M

Y

D

T

A

A

L

L

E

K

E

A

L

M

D

R

N

E

D

N

P

E

V

V

I

I

T

K

E

K

A

A

L

L

G

G

N

N

H

H

L

I

Y

Y

E

N

H

Q

F

F

V

I

E

N

A

S

K

K

Q

S

I

I

E

E

W

W

D

D

M

Y

F

Y

R

R

T

T

A

Q

V

V

H

S

P

E

W

W

E

E

R

R

E

D

Q

Q

Y

Y

L

M

T

K

S

Q

Y

Y

binding adenosine-5'-diphosphate: G129 (= G131), E131 (= E133), E183 (= E185), D197 (= D199), F198 (= F200), K199 (= K201), Y200 (= Y202), N246 (= N248), V247 (≠ M249), S248 (= S250), R320 (= R322), S328 (= S330), R330 (= R332)
binding magnesium ion: E131 (= E133), E131 (= E133), E133 (= E135), E188 (= E190), E195 (= E197), E195 (= E197), H244 (= H246), E332 (= E334)
binding l-methionine-s-sulfoximine phosphate: E131 (= E133), E133 (= E135), E188 (= E190), E195 (= E197), G240 (= G242), H244 (= H246), R297 (= R299), E303 (= E305), R315 (= R317)

7tf6A Glutamine synthetase (see paper)
73% identity, 99% coverage: 7:445/445 of query aligns to 4:438/438 of 7tf6A

query
sites
7tf6A

K

K

Q

E

D

D

I

I

Y

R

K

K

M

F

I

A

D

E

E

E

S

N

V

V

R

R

F

Y

I

L

R

R

L

L

Q

Q

F

F

T

T

D

D

M

I

L

L

G

G

T

T

I

I

K

K

N

N

V

V

E

E

I

V

P

P

Y

V

S

S

Q

Q

L

L

E

E

K

K

A

V

F

L

D

D

N

N

K

E

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
|
F

V

V

R
|
R

I

I

D

E

E

E

S

S

D

D

M

M

Y

Y

L

L

Y

H

P

P

D

D

L

L

D

D

T

T

F

W

V

V

V

I

F

F

P

P

W

W

T

-

A

-

E

-

K

-

G

G

K

K

V

V

A

A

R

R

F

L

I

I

C

C

D

D

I

V

Y

Y

N

K

P

T

D

D

G

G

T

T

P

P

F

F

E

E

G

G

C

D

P

P

R

R

Y

A

N

N

L

L

K

K

R

R

N

V

L

L

K

K

R

E

M

M

E

E

E

D

L

L

G

G

Y

F

S

T

A

D

F

F

N

N

I

L

G

G

T

P

E
|
E

P

P

E
|
E

F

F

L

L

F

F

K

K

L

L

D

D

E

E

K

K

G

G

D

E

P

P

M

T

M

L

E

E

L

L

N

N

D

D

R

D

G

G

Y

Y

F

F

D

D

L

L

A

A

P

P

T

T

D

D

L

L

G

G

E

E

N

N

C

C

R

R

D

D

I

I

V

V

L

L

E

E

L

L

E

E

E

D

M

M

G

G

F

F

E

D

I

I

E

E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

D

A

A

V

V

K

T

H

A

A

C

D

D

D

N

I

I

Q

Q

T

T

F

F

K

K

L

L

V

V

K

K

T

T

I

I

A

A

R

R

K

K

H

H

N

N

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

F

F

G
|
G

V

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

V

F

N

N

M

V

S

S

L

L

F

F

K

K

D

G

G

K

R

E

N

N

V

A

F

F

Y

F

D

D

E

P

K

N

G

T

D

E

M

M

Q

G

V

L

S

T

T

E

T

T

L

A

K

Y

Q

Q

F

F

T

T

A

A

G

G

I

V

I

L

K

K

H

N

A

A

T

R

N

G

F

F

T

T

A

A

V

V

T

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

C

C

Y

Y

V

I

A

A

W

W

S

S

G

G

L

K

N

N

R
|
R

S

S

P

P

L

L

V

I

R

R

V

V

P

P

S

S

R

R

G

G

L

L

S

S

T

T

R

R

I

I

E
|
E

A

V

R

R

S

S

V

V

D

D

P

P

A

A

N

N

P

P

Y

Y

M

M

T

A

L

L

A

A

V

A

L

I

L

L

S

E

A

A

G

G

L

L

D

D

G

G

I

I

E

K

N

N

K

K

L

L

T

K

P

V

P

P

E

E

P

P

V

V

D

N

Q

Q

N

N

I

I

Y
|
Y

E

E

M

M

D

N

K

R

A

E

E

E

R

R

E

E

E

A

H

V

G

G

I

I

K

Q

S

D

L

L

P

P

A

S

T

T

L

L

M

Y

D

T

A

A

L

L

E

K

E

A

L

M

D

R

N

E

D

N

P

E

V

V

I

I

T

K

E

K

A

A

L

L

G

G

N

N

H

H

L

I

Y

Y

E

N

H

Q

F

F

V

I

E

N

A

S

K

K

Q

S

I

I

E

E

W

W

D

D

M
x
Y

F

Y

R

R

T

T

A

Q

V

V

H

S

P

E

W

W

E

E

R

R

E

D

Q
|
Q

Y

Y

L

M

T

K

S
x
Q

Y

Y

binding glutamine: E128 (= E135), E183 (= E190), G235 (= G242), H239 (= H246), R292 (= R299), E298 (= E305)
binding magnesium ion: E126 (= E133), E128 (= E135), E183 (= E190), E190 (= E197), H239 (= H246), E327 (= E334)
binding : F58 (= F61), R60 (= R63), G232 (= G239), N234 (= N241), G296 (= G303), Y297 (= Y304), R310 (= R317), Y367 (= Y374), Y421 (≠ M428), Q433 (= Q440), Q437 (≠ S444)

7tfaB Glutamine synthetase (see paper)
72% identity, 99% coverage: 6:445/445 of query aligns to 3:441/441 of 7tfaB

query
sites
7tfaB

N

T

K

R

Q

E

D

D

I

I

Y

I

K

R

M

I

I

A

D

E

E

E

S

N

V

V

R

R

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

M

L

L

L

G

G

T

T

I

I

K

K

N

N

V

V

E

E

I

I

P

P

Y

V

S

S

Q

Q

L

L

E

E

K

K

A

A

F

L

D

D

N

N

K

K

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F
x
Y

V

V

R
|
R

I

I

D

E

E

E

S

S

D

D

M

M

Y

Y

L

L

Y

Y

P

P

D

D

L

L

D

D

T

T

F

W

V

V

F

F

P

P

W

W

T

V

A

T

E

S

K

D

G

-

K

R

V

V

A

A

R

R

F

L

I

I

C

C

D

D

I

I

Y

Y

N

K

P

P

D

D

G

G

T

S

P

P

F

F

E

A

G

G

C

D

P

P

R

R

Y

G

N

I

L

L

K

K

R

R

N

V

L

L

K

K

R

E

M

A

E

E

L

L

G

G

Y

Y

S

T

A

S

F

M

N

N

I

V

G

G

T

P

E
|
E

P

P

E
|
E

F

F

L

L

F

F

K

K

L

T

D

D

E

E

K

K

G

G

D

D

P

P

M

T

E

E

L

L

N

N

D

D

R

Q

G

G

Y
|
Y

F

F

D

D

L

L

A

A

P

P

T

M

D

D

L

L

G

G

E

E

N

N

C

C

R

R

D

E

I

I

V

V

L

L

E

K

L

L

E

E

M

M

G

G

F

F

E

E

I

I

E

E

A

A

S

S

H

H

E
|
E

V
|
V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D

D

F

F

K

K

Y

Y

S

A

D

D

A

A

V

V

K

K

H

A

A

A

D

D

D

Q

I

I

Q

Q

T

T

F

F

K

K

L

L

V

V

K

K

T

T

I

I

A

A

R

R

K

Q

H

H

N

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

F

F

G

G

V

V

N
|
N

G
|
G

S

S

G

G

M

M

H
|
H

V

C

N

N

M

Q

S

S

L

L

F

F

K

K

D

D

G

N

R

E

N

N

V

V

F

F

Y

Y

D

D

E

E

K

T

G

D

D

E

M

L

Q

G

V

L

S

S

T

Q

T

T

L

A

K

R

Q

H

F

Y

T

M

A

A

G

G

I

I

I

L

K

K

H

H

A

A

T

R

N

A

F

M

T

A

A

A

V

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G
|
G

Y
|
Y

E
|
E

A

A

P

P

C

C

Y

Y

V

V

A

A

W

W

S

S

G

A

L

S

N

N

R
|
R

S

S

P

P

L

M

V

I

R

R

V

I

P

P

S

A

S

S

R

R

G

G

L

L

S

S

T

T

R

R

I

V

E
|
E

A

V

R

R

S

N

V

P

D

D

P

P

A

A

N

N

P

P

Y

Y

M

L

T

A

L

L

A

A

V

V

L

M

L

L

S

R

A

A

G

G

L

L

D

D

G

G

I

I

E

K

N

R

K

Q

L

M

T

A

P

L

P

P

E

A

P

P

V

I

D

D

Q

R

N

N

I

I

Y

Y

E

V

M

M

D

S

K

E

A

E

E

E

R

R

E

I

E

E

H

E

G

G

I

I

K

P

S

S

L

L

P

P

A

A

T

D

L

L

M

K

D

E

A

A

L

L

E

S

E

E

L

L

D

I

N

R

D

S

P

E

V

V

I

I

T

S

E

D

A

A

L

L

G

G

N

D

H

H

L

A

Y

L

E

A

H

Y

F

F

V

Y

E

E

A

L

K

K

Q

E

I

I

E

E

W

W

D

D

M
|
M

F

Y

R

R

T

T

A

Q

V

V

H

H

P

Q

W

W

E

E

R

R

E

D

Q

Q

Y

Y

L

L

T

T

S

L

Y

Y

binding glutamine: E131 (= E135), Y153 (= Y157), E186 (= E190), G238 (= G242), H242 (= H246), R295 (= R299), E301 (= E305)
binding magnesium ion: E129 (= E133), E131 (= E135), E186 (= E190), E193 (= E197), H242 (= H246), E330 (= E334)
binding : Y58 (≠ F61), R60 (= R63), V187 (= V191), N237 (= N241), G299 (= G303), Y300 (= Y304), R313 (= R317), M424 (= M428)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
72% identity, 99% coverage: 6:445/445 of query aligns to 3:439/439 of 7tdpA

query
sites
7tdpA

N

T

K

R

Q

E

D

D

I

I

Y

I

K

R

M

I

I

A

D

E

E

E

S

N

V

V

R

R

F

F

I

I

R

R

L

L

Q

Q

F

F

T

T

D

D

M

L

L

L

G

G

T

T

I

I

K

K

N

N

V

V

E

E

I

I

P

P

Y

V

S

S

Q

Q

L

L

E

E

K

K

A

A

F

L

D

D

N

N

K

K

M

M

F

F

D

D

G

G

S

S

I

I

E

E

G

G

F

Y

V

V

R

R

I

I

D

E

E

E

S

S

D

D

M

M

Y

Y

L

L

Y

Y

P

P

D

D

L

L

D

D

T

T

F

W

V

V

F

F

P

P

W

W

T

S

A

-

E

-

K

-

G

D

K

R

V

V

A

A

R

R

F

L

I

I

C

C

D

D

I

I

Y

Y

N

K

P

P

D

D

G

G

T

S

P

P

F

F

E

A

G

G

C

D

P

P

R

R

Y

G

N

I

L

L

K

K

R

R

N

V

L

L

K

K

R

E

M

A

E

E

L

L

G

G

Y

Y

S

T

A

S

F

M

N
|
N

I

V

G
|
G

T

P

E
|
E

P

P

E
|
E

F

F

L

L

F

F

K

K

L

T

D

D

E

E

K

K

G

G

D

D

P

P

M

T

E

E

L

L

N

N

D

D

R

Q

G

G

Y

Y

F

F

D

D

L

L

A

A

P

P

T

M

D

D

L

L

G

G

E

E

N

N

C

C

R

R

D

E

I

I

V

V

L

L

E

K

L

L

E

E

M

M

G

G

F

F

E

E

I

I

E
|
E

A

A

S

S

H

H

E
|
E

V

V

A

A

P

P

G

G

Q

Q

H

H

E
|
E

I

I

D
|
D

F

F

K

K

Y
|
Y

S

A

D

D

A

A

V

V

K

K

H

A

A

A

D

D

D

Q

I

I

Q

Q

T

T

F

F

K

K

L

L

V

V

K

K

T

T

I

I

A

A

R

R

K

Q

H

H

N

G

L

L

H

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

L

L

F

F

G

G

V

V

N

N

G
|
G

S

S

G

G

M

M

H
|
H

V

C

N
|
N

M

Q

S
|
S

L

L

F

F

K

K

D

D

G

N

R

E

N

N

V

V

F

F

Y

Y

D

D

E

E

K

T

G

D

D

E

M

L

Q

G

V

L

S

S

T

Q

T

T

L

A

K

R

Q

H

F

Y

T

M

A

A

G

G

I

I

I

L

K

K

H

H

A

A

T

R

N

A

F

M

T

A

A

A

V

I

T

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

V

V

P

P

G

G

Y

Y

E
|
E

A

A

P

P

C

C

Y

Y

V

V

A

A

W

W

S

S

G

A

L

S

N

N

R
|
R

S

S

P

P

L

M

V

I

R
|
R

V

I

P

P

S

A

S

S

R

R

G

G

L

L

S

S

T

T

R
|
R

I

V

E
|
E

A

V

R
|
R

S

N