SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_029910362.1 NCBI__GCF_000711315.1:WP_029910362.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
47% identity, 99% coverage: 1:248/250 of query aligns to 1:248/249 of P12282

query
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P12282

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R14 (= R14) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S44) mutation to A: No effect.
R73 (= R73) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (= C128) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D130) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C142) mutation to A: No effect.
C172 (= C173) mutation to A: No zinc bound and no enzyme activity.
C175 (= C176) mutation to A: No zinc bound and no enzyme activity.
C187 (= C188) mutation to A: No effect.
C231 (≠ M231) mutation to A: No effect.
C244 (= C244) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C247) mutation to A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
46% identity, 98% coverage: 4:248/250 of query aligns to 3:240/240 of 1jwbB

query
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1jwbB

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active site: R13 (= R14), D129 (= D130)
binding adenosine monophosphate: G37 (= G38), G39 (= G40), G40 (= G41), D61 (= D62), F62 (= F63), K85 (= K86), L108 (≠ N109), C127 (= C128), T128 (= T129), D129 (= D130), N130 (= N131), V133 (≠ T134)
binding zinc ion: C171 (= C173), C236 (= C244), C239 (= C247)

1jw9B Structure of the native moeb-moad protein complex (see paper)
46% identity, 98% coverage: 4:248/250 of query aligns to 3:240/240 of 1jw9B

query
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1jw9B

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active site: R13 (= R14), D129 (= D130)
binding zinc ion: C171 (= C173), C236 (= C244), C239 (= C247)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
42% identity, 98% coverage: 4:249/250 of query aligns to 64:311/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
44% identity, 95% coverage: 4:240/250 of query aligns to 3:217/217 of 1jwaB

query
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1jwaB

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V

V

I

G

G

S

S

I

L

Q

Q

A

A

I

M

E

E

A

A

I

I

K

K

A

M

L

L

-

A

-

G

L

Y

D

G

K

K

P

P

T

A

L

-

T

S

G

G

K

K

L

I

M

V

I

M

L

Y

D

D

A

A

Y

M

T

T

M

C

N

Q

I

F

R

R

T

E

L

M

N

K

L

L

K

M

K

R

active site: R13 (= R14), D129 (= D130)
binding adenosine-5'-triphosphate: G39 (= G40), G40 (= G41), D61 (= D62), F62 (= F63), R72 (= R73), K85 (= K86), L108 (≠ N109), D129 (= D130), N130 (= N131), V133 (≠ T134)

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
40% identity, 98% coverage: 4:249/250 of query aligns to 61:320/482 of O59954

query
sites
O59954

L

L

N

H

D

G

D

E

E

E

L

Y

S

R

R

R

Y

Y

S

G

R

R

Q

Q

I

M

L

I

L

V

S

P

E

Q

V

F

D

G

Y

L

D

Q

G
|
G

Q

Q

L

L

K

K

L

L

T

R

Q

D

S

A

H

K

A

V

V

L

I

I

V

V

G

G

L

A

G

G

L

L

G
|
G

S

C

P

P

V

A

S

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

S

G

L

L

I

V

D

D

F

G

D

D

N

T

V

V

D

E

D

A

S

S

N

N

L

L

Q

H

R
|
R

Q

Q

I

V

V

L

H

H

R

R

E

S

N

R

N

N

I

V

G

G

M

K

A

L

K

K

V

V

R

D

S

S

A

A

E

I

E

E

N

Y

L

L

K

R

A

E

L

L

N

N

H

P

F

H

V

P

D

T

I

Y

F

I

A

A

Y

H

D

Q

Y

A

N

H

L

L

S

T

E

P

Q

R

E

E

F

A

E

P

A

D

I

I

I

F

E

K

K

D

A

Y

D

D

V

L

V

I

I

L

D

D

C
|
C

T

T

D

D

N

N

F

P

S

A

T

T

R

R

F

Y

M

L

L

I

N

S

R

D

L

T

C

A

Y

V

A

L

Q

L

K

G

K

K

P

P

L

L

I

V

S

S

G

A

A

S

A

A

I

L

R

R

W

T

E
|
E

G

G

Q

Q

L

L

S

M

T

V

F

L

D

N

F

N

R

P

-

P

-

Q

-

P

-

G

-

D

K

K

D

T

T

G

S

G

P

P

C

C

Y

Y

Q

R

C

C

L

V

Y

F

-

P

K

K

E

P

D

P

S

P

G

A

Q

N

E

S

L

V

T

T

-

S

C

C

S

A

E

D

N

G

G

G

V

I

L

L

A

G

P

P

V

V

G
|
G

M

T

V

M

G

G

S

V

I

L

Q

Q

A

A

I

S

E

E

A

A

I

I

K

K

A

V

L

L

L

T

D

S

K

A

-

G

-

E

-

S

-

V

P

E

T

A

L

T

T

P

G

P

K

S

L

L

M

L

I

I

L

F

D

S

A

A

Y

Y

-

S

T

S

M

P

N

Q

I

F

R

R

T

T

L

I

N

K

L

L

K

R

-

S

K

R

D

R

P

P

H

N

C

C

P

A

I

V

C

C

S

S

S

A

G82 (= G25) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G43) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R73) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (= C128) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (= E158) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G199) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 99% coverage: 4:250/250 of query aligns to 1:246/251 of P30138

query
sites
P30138

L

M

N

N

D

D

D

R

E

D

L

F

S

M

R

R

Y

Y

S

S

R

R

Q

Q

I

I

L

L

S

D

E

D

V

I

D

A

Y

L

D

D

G

G

Q

Q

L

Q

K

K

L

L

T

L

Q

D

S

S

H

Q

A

V

V

L

I

I

V

I

G

G

L

L

G

G

L

L

G

G

S

T

P

P

V

A

S

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

S

V

L

L

I

A

D

D

F

D

D

D

N

D

V

V

D

H

D

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

V

L

H

F

R

T

E

T

N

E

N

D

I

I

G

D

M

R

A

P

K

K

V

S

R

Q

S

V

A

S

E

Q

N

R

L

L

K

T

A

Q

L

L

N

N

H

P

F

D

V

I

D

Q

I

L

F

T

A

A

Y

L

D

Q

Y

Q

N

R

L

L

S

T

E

G

Q

E

E

A

F

L

E

K

A

D

I

A

I

V

E

A

K

R

A

A

D

D

V

V

I

L

D

D

C

C

T

T

D

D

N

N

F

M

S

A

T

T

R

R

F

Q

M

E

L

I

N

N

R

A

L

A

C

C

Y

V

A

A

Q

L

K

N

K

T

P

P

L

L

I

I

S

T

G

A

A

S

A

A

I

V

R

G

W

F

E

G

G

G

Q

Q

L

L

S

M

T

V

F

L

D

-

F

-

R

-

K

-

D

-

T

T

S

P

P

P

-

W

-

E

-

Q

-

G

C
|
C

Y

Y

Q

R

C
|
C

L

L

Y
x
W

K

P

E

D

D

N

S

Q

G

E

Q

P

E

E

L

R

T

N

C
|
C

S

R

E

T

N

A

G

G

V

V

L

V

A

G

P

P

V

V

G

G

M

V

V

M

G

G

S

T

I

L

Q

Q

A

A

I

L

E

E

A

A

I

I

K

K

A

L

L

L

L

S

D

G

K

I

P

E

T

T

L

P

T

A

G

G

K

E

L

L

M

R

I

L

L

F

D

D

A

G

Y

K

T

S

M

S

N

Q

I

W

R

R

T

S

L

L

N

A

L

L

K

R

D

A

P

S

H

G

C
|
C

P

P

I

V

C
|
C

S

G

N

S

C169 (= C173) binding Zn(2+)
C172 (= C176) binding Zn(2+)
W174 (≠ Y178) mutation to A: No adenylation of ThiS.
C184 (= C188) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C244) binding Zn(2+)
C243 (= C247) binding Zn(2+)

1zfnA Structural analysis of escherichia coli thif (see paper)
42% identity, 98% coverage: 4:248/250 of query aligns to 1:244/244 of 1zfnA

query
sites
1zfnA

L

M

N

N

D

D

D

R

E

D

L

F

S

M

R

R

Y

Y

S

S

R
|
R

Q

Q

I

I

L

L

S

D

E

D

V

I

D

A

Y

L

D

D

G

G

Q

Q

L

Q

K

K

L

L

T

L

Q

D

S

S

H

Q

A

V

V

L

I

I

V
x
I

G
|
G

L

L

G
|
G

L

L

G

G

S

T

P

P

V

A

S

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

S

V

L

L

I

A

D
|
D

F

D

D

D

N

D

V

V

D

H

D

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

I

I

V

L

H

F

R

T

E

T

N

E

N

D

I

I

G

D

M

R

A

P

K
|
K

V

S

R

Q

S

V

A

S

E

Q

N

R

L

L

K

T

A

Q

L

L

N

N

H

P

F

D

V

I

D

Q

I

L

F

T

A

A

Y

L

D

Q

Y

Q

N

R

L

L

S

T

E

G

Q

E

E

A

F

L

E

K

A

D

I

A

I

V

E

A

K

R

A

A

D

D

V

V

I

L

D

D

C

C

T
|
T

D
|
D

N

N

F

M

S

A

T
|
T

R

R

F

Q

M

E

L

I

N

N

R

A

L

A

C

C

Y

V

A

A

Q

L

K

N

K

T

P

P

L

L

I

I

S

T

G

A

A

S

A

A

I

V

R

G

W

F

E

G

G

G

Q

Q

L

L

S

M

T

V

F

L

D

-

F

-

R

-

K

-

D

-

T

T

S

P

P

P

-

W

-

E

-

Q

-

G

C
|
C

Y

Y

Q

R

C
|
C

L

L

Y

W

K

P

E

D

D

N

S

Q

G

E

Q

P

E

E

L

R

T

N

C

C

S

R

E

T

N

A

G

G

V

V

L

V

A

G

P

P

V

V

G

G

M

V

V

M

G

G

S

T

I

L

Q

Q

A

A

I

L

E

E

A

A

I

I

K

K

A

L

L

L

L

S

D

G

K

I

P

E

T

T

L

P

T

A

G

G

K

E

L

L

M

R

I

L

L

F

D

D

A

G

Y

K

T

S

M

S

N

Q

I

W

R

R

T

S

L

L

N

A

L

L

K

R

D

A

P

S

H

G

C
|
C

P

P

I

V

C
|
C

S

G

active site: R11 (= R14), D127 (= D130)
binding adenosine-5'-triphosphate: I34 (≠ V37), G35 (= G38), G37 (= G40), G38 (= G41), D59 (= D62), R70 (= R73), Q71 (= Q74), K83 (= K86), T126 (= T129), D127 (= D130), T131 (= T134)
binding zinc ion: C169 (= C173), C172 (= C176), C240 (= C244), C243 (= C247)

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
39% identity, 86% coverage: 2:216/250 of query aligns to 5:215/289 of 6yubB

query
sites
6yubB

K

K

S

S

L

L

N

S

D

K

D

E

E

E

L

I

S

E

R

R

Y

Y

S

S

R

R

Q

Q

I

M

L

I

L

V

S

P

E

G

V

M

D

G

Y

K

D

E

G

G

Q

Q

L

L

K

R

L

L

T

M

Q

N

S

A

H

K

A

V

V

L

I

I

V

I

G

G

L

A

G

G

L

L

G

G

S

C

P

P

V

A

S

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

S

G

L

I

I

V

D

D

F

G

D

D

N

S

V

V

D

E

D

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

V

A

H

H

R

A

E

T

N

K

N

R

I

V

G

G

M

M

A

L

K

K

V

V

R

D

S

S

A

L

E

I

E

T

N

H

L

L

K

I

A

E

L

I

N

N

H

P

F

L

V

P

D

V

I

Y

F

V

A

P

Y

Y

D

R

Y

F

N

D

L

L

S

T

E

P

Q

Q

E

N

F

A

E

A

A

Q

I

I

E

K

K

P

A

W

D

D

V

V

V

I

I

L

D

D

C

C

T

T

D

D

N

N

F

P

S

A

T

T

R

R

F

Y

M

L

L

I

N

S

R

D

L

V

C

C

Y

V

A

L

Q

L

K

G

K

K

P

P

L

L

I

V

S

S

G

A

A

A

A

S

I

V

R

Q

W

K

E

S

G

G

Q

Q

L

L

S

I

T

V

F

L

D

N

-

C

-

P

-

T

-

P

-

Q

-

G

-

V

F

V

R

N

K

K

D

K

T

A

S

A

P

P

C
|
C

Y

Y

Q

R

C
|
C

L

C

Y

F

K

K

E

-

D

-

S

-

G

-

Q

-

E

-

L

-

T

-

C

-

S

-

E

-

N

-

G

G

V

I

L

M

A

G

P

P

V

V

G

G

M

M

V

M

G

G

S

V

I

A

Q

Q

A

A

I

G

E

E

A

A

I

I

K

K

A

I

L

L

L

V

D

S

K

Q

binding zinc ion: C184 (= C173), C187 (= C176)

Sites not aligning to the query:

binding zinc ion: 265

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
39% identity, 86% coverage: 2:216/250 of query aligns to 4:216/423 of 6yubA

query
sites
6yubA

K

K

S

S

L

L

N

S

D

K

D

E

E

E

L

I

S

E

R

R

Y

Y

S

S

R

R

Q

Q

I

M

L

I

L

V

S

P

E

G

V

M

D

G

Y

K

D

E

G

G

Q

Q

L

L

K

R

L

L

T

M

Q

N

S

A

H

K

A

V

V

L

I

I

V

I

G

G

L

A

G

G

L

L

G

G

S

C

P

P

V

A

S

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

S

G

L

I

I

V

D

D

F

G

D

D

N

S

V

V

D

E

D

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

V

A

H

H

R

A

E

T

N

K

N

R

I

V

G

G

M

M

A

L

K

K

V

V

R

D

S

S

A

L

E

I

E

T

N

H

L

L

K

I

A

E

L

I

N

N

H

P

F

L

V

P

D

V

I

Y

F

V

A

P

Y

Y

D

R

Y

F

N

D

L

L

S

T

E

P

Q

Q

E

N

F

A

E

A

A

Q

I

I

E

K

K

P

A

W

D

D

V

V

V

I

I

L

D

D

C

C

T

T

D

D

N

N

F

P

S

A

T

T

R

R

F

Y

M

L

L

I

N

S

R

D

L

V

C

C

Y

V

A

L

Q

L

K

G

K

K

P

P

L

L

I

V

S

S

G

A

A

A

A

S

I

V

R

Q

W

K

E

S

G

G

Q

Q

L

L

S

I

T

V

F

L

D

N

-

C

-

P

-

T

-

P

-

Q

-

G

-

V

F

V

R

N

K

K

D

K

T

A

S

A

P

P

C
|
C

Y

Y

Q

R

C
|
C

L

C

Y

F

K

K

E

K

D

P

S

-

G

-

Q

-

E

-

L

-

T

-

C

-

S

-

E

-

N

-

G

G

V

I

L

M

A

G

P

P

V

V

G

G

M

M

V

M

G

G

S

V

I

A

Q

Q

A

A

I

G

E

E

A

A

I

I

K

K

A

I

L

L

L

V

D

S

K

Q

binding zinc ion: C183 (= C173), C186 (= C176)

Sites not aligning to the query:

binding zinc ion: 266, 269

1zud3 Structure of this-thif protein complex (see paper)
42% identity, 98% coverage: 4:249/250 of query aligns to 1:240/240 of 1zud3

query
sites
1zud3

L

M

N

N

D

D

D

R

E

D

L

F

S

M

R

R

Y

Y

S

S

R
|
R

Q

Q

I

I

L
|
L

L

L

S
x
D

E

D

V

I

D

A

Y

L

D

D

G

G

Q

Q

L

Q

K

K

L

L

T

L

Q

D

S

S

H

Q

A

V

V

L

I

I

V

I

G

G

L

L

G

G

L

L

G

G

S

T

P

P

V

A

S

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

S

V

L

L

I

A

D

D

F

D

D

D

N

D

V

V

D

H

D

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

V

L

H

F

R

T

E

T

N

E

N

D

I

I

G

D

M

R

A

P

K

K

V

S

R

Q

S

V

A

S

E

Q

N

R

L

L

K

T

A

Q

L

L

N

N

H

P

F

D

V

I

D

Q

I

L

F

T

A

A

Y

L

D

Q

Y

Q

N

R

L

L

S

T

E

G

Q

E

E

A

F

L

E

K

A

D

I

A

I

V

E

A

K

R

A

A

D

D

V

V

I

L

D

D

C

C

T

T

D
|
D

N

N

F

M

S

A

T

T

R

R

F

Q

M

E

L

I

N

N

R

A

L

A

C

C

Y

V

A

A

Q

L

K

N

K

T

P

P

L

L

I

I

S

T

G

A

A

S

A

A

I

V

R

G

W

F

E

G

G

G

Q

Q

L

L

S

M

T

V

F

L

D

-

F

-

R

-

K

-

D

-

T

T

S

P

P

P

-

W

-

E

-

Q

-

G

C
|
C

Y

Y

Q

R

C

C

L

L

Y

W

K

P

E

D

D

N

S

-

G

-

Q

Q

E

E

L

P

T

T

C

A

S

G

E

V

N

V

G

G

V

P

L

-

A

-

P

-

V

V

G

G

M

V

V

M

G

G

S

T

I

L

Q

Q

A

A

I

L

E

E

A

A

I

I

K

K

A

L

L

L

L

S

D

G

K

I

P

E

T

T

L

P

T

A

G

G

K

E

L

L

M

R

I

L

L

F

D

D

A

G

Y

K

T

S

M

S

N

Q

I

W

R

R

T

S

L

L

N

A

L

L

K

R

D

A

P

S

H

G

C
|
C

P

P

I

V

C
|
C

S

G

active site: R11 (= R14), D127 (= D130)
binding calcium ion: L14 (= L17), D16 (≠ S19)
binding zinc ion: C169 (= C173), C235 (= C244), C238 (= C247)

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
41% identity, 90% coverage: 3:228/250 of query aligns to 54:280/460 of O95396

query
sites
O95396

S

A

L

L

N

S

D

R

D

D

E

E

L

I

S

L

R

R

Y

Y

S

S

R

R

Q

Q

I

L

L

V

L

L

S

P

E

E

V

L

D

G

Y

V

D

H

G

G

Q

Q

L

L

K

R

L

L

T

G

Q

T

S

A

H

C

A

V

V

L

I

I

V

V

G

G

L

C

G

G

L

L

G

G

S

C

P

P

V

L

S

A

L

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

T

R

L

L

S

G

L

L

I

V

D

D

F

Y

D

D

N

V

V

V

D

E

D

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

I

V

V

L

H

H

R

G

E

E

N

A

N

L

I

A

G

G

M

Q

A

A

K

K

V

A

R

F

S

S

A

A

E

A

N

S

L

L

K

R

A

R

L

L

N

N

H

S

F

A

V

V

D

E

I

C

F

V

A

P

Y

Y

D
x
T

Y
x
Q

N
x
A

L
|
L

S
x
T

E
x
P

Q
x
A

E
x
T

F
x
A

E
x
L

A
x
D

I
x
L

I
x
V

E
x
R

K
x
R

A
x
Y

D
|
D

V
|
V

I
x
A

D
|
D

C
|
C

T
x
S

D
|
D

N
|
N

F
x
V

S
x
P

T
|
T

R
|
R

F
x
Y

M
x
L

L
x
V

N
|
N

R
x
D

L
x
A

C
|
C

Y
x
V

A
x
L

Q
x
A

K
x
G

K
x
R

P
|
P

L
|
L

I
x
V

S
|
S

G
x
A

A
x
S

A
|
A

I
x
L

R
|
R

W
x
F

E
|
E

G
|
G

Q
|
Q

L
x
I

S
x
T

T
x
V

F
x
Y

D
x
H

F
x
Y

R

-

K

-

D
|
D

T
x
G

S
x
G

P
|
P

C
|
C

Y
|
Y

Q
x
R

C
|
C

L
x
I

Y
x
F

K
x
P

E
x
Q

D
x
P

S
x
P

G
x
P

Q
x
A

E
|
E

L
x
T

T
x
V

-
x
T

-
x
N

C
|
C

S

A

E

D

N

G

G

G

V

V

L

L

A

G

P

V

V

V

V

T

G

G

M

V

V

L

G

G

S

C

I

L

Q

Q

A

A

I

L

E

E

A

V

I

L

K

K

-

I

A

A

L

A

L

G

D

L

K

G

P

P

T

S

L

Y

T

S

G

G

K

S

L

L

M

L

I

L

L

F

D

D

A

A

158:238 (vs. 107:187, 31% identical) Interaction with NFS1
C239 (= C188) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
41% identity, 99% coverage: 3:250/250 of query aligns to 4:251/270 of D4GSF3

query
sites
D4GSF3

S

S

L

L

N

D

D

A

D

T

E

Q

L

L

S

D

R

R

Y

Y

S

S

R

R

Q

H

I

I

L

I

L

M

S

D

E

E

V

V

D

G

Y

P

D

E

G

G

Q

Q

L

G

K

R

L

L

T

L

Q

S

S

S

H

R

A

V

V

V

I

V

V

V

G

G

L

A

G

G

L

L

G

G

S

A

P

P

V

A

S

I

L

Q

Y

Y

L

L

A

A

A

V

G

G

V

V

G

G

T

E

L

L

S

V

L

V

I

V

D

D

F

D

D

D

N

V

V

V

D

E

D

R

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

V

V

V

H

H

R

C

E

D

N

D

I

V

G

G

M

T

A

P

K

K

V

A

R

E

S

S

A

A

E

A

N

F

L

V

K

R

A

G

L

L

N

N

H

P

F

D

V

V

D

S

I

V

F

E

A

P

Y

V

D

E

Y

A

N

R

L

V

S

D

E

K

Q

S

E

N

F

V

E

H

A

E

I

V

E

A

K

G

A

S

D

D

V

V

I

V

D

D

C

A

T

S

D

D

N

N

F

F

S

P

T

T

R

R

F

Y

M

L

L

L

N

N

R

D

L

V

C

C

Y

R

A

F

Q

E

K

G

K

I

P

P

L

L

I

V

S

H

G

G

A

A

A

I

I

Y

R

K

W

F

E

E

G

G

Q

Q

L

A

S

T

T

T

F

L

D

-

F

-

R

-

K

V

D

P

T

D

S

G

P

P

C

C

Y

Y

Q

R

C

C

L

L

Y

F

K

P

E

E

-

A

-

P

D

E

S

P

G

G

Q

T

E

V

L

P

T

D

C
|
C

S

A

E

T

N

T

G

G

V

V

L

L

A

G

P

V

V

L

V

P

G

G

M

T

V

V

G

G

S

C

I

I

Q

Q

A

A

I

T

E

E

A

A

I

M

K

K

A

L

L

L

D

D

K

E

-

G

P

E

T

A

L

L

T

D

G

G

K

R

L

L

M

L

I

F

L

Y

D

D

A

A

Y

M

T

D

M

M

N

T

I

F

R

E

T

T

L

V

N

P

L

Y

K

R

K

T

D

N

P

P

H

D

C

C

P

P

I

V

C

C

S

G

S

E

N

G

C188 (= C188) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
38% identity, 98% coverage: 5:248/250 of query aligns to 4:253/271 of Q72J02

query
sites
Q72J02

N

T

D

K

D

E

E

E

L

L

S

D

R

R

Y

Y

S

H

R

R

Q

Q

I

M

L

I

L

L

S

P

E

Q

V

V

D

G

Y

P

D

E

G

G

Q

Q

L

E

K

R

L

L

T

K

Q

R

S

A

H

S

A

V

V

V

I

V

V

V

G

G

L

A

G

G

L

L

G

G

S

V

P

P

V

V

S

L

L

Q

Y

Y

L

L

A

V

A

A

G

G

V

V

G

G

T

R

L

V

S

G

L

V

I

V

D

E

F

M

D

D

N

R

V

V

D

E

D

V

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

V

L

H

Y

R

T

E

T

N

E

N

D

I

V

G

G

M

E

A

P

K

K

V

A

R

L

S

V

A

A

E

Q

E

K

N

R

L

L

K

Q

A

A

L

L

N

N

H

P

F

L

V

V

D

R

I

V

F

E

A

A

Y

Y

D

P

Y

V

N

R

L

L

S

T

E

S

Q

E

E

N

F

A

E

L

A

E

I

I

I

L

E

R

K

P

A

Y

D

D

V

L

V

V

I

V

D

D

C

A

T

S

D

D

N

N

F

F

S

P

T

T

R

R

F

Y

M

L

L

V

N

N

R

D

L

A

C

A

Y

V

A

L

Q

L

K

G

K

K

P

P

L

L

I

V

S

F

G

G

A

A

A

I

I

Y

R

Q

W

F

E

D

G

G

Q

Q

L

V

S

A

T

V

F

F

D

H

-

H

-

P

-

T

F

L

R

H

K

G

D

E

T

M

S

G

P

P

C

C

Y

Y

Q

R

C

C

L

L

Y

F

-

P

-

K

K

P

E

P

D

P

S

P

G

G

Q

A

E

V

L

P

T

S

C
|
C

S

A

E

E

N

A

G

G

V

V

L

F

A

G

P

V

V

L

V

P

G

A

M

V

V

V

G

G

S

S

I

L

Q

M

A

A

I

A

E

E

A

A

I

L

K

K

A

V

L

L

-

G

-

I

D

G

K

K

P

P

T

-

L

L

T

A

G

G

K

H

L

L

M

L

I

L

L

Y

D

D

A

A

Y

L

T

E

M

A

N

S

I

F

R

R

T

K

L

L

N

T

L

V

K

R

D

N

P

P

H

R

C

C

P

P

I

V

C

C

S

G

C192 (= C188) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
32% identity, 99% coverage: 4:250/250 of query aligns to 39:292/440 of P38820

query
sites
P38820

L

L

N

S

D

L

D

E

E

E

L

Y

S

Q

R

R

Y

Y

S

G

R

R

Q

Q

I

M

L

I

L

V

S

E

E

E

V

T

D

G

-

G

Y

V

D

A

G

G

Q

Q

L

V

K

K

L

L

T

K

Q

N

S

T

H

K

A

V

V

L

I

V

V

V

G

G

L

A

G

G

L

L

G

G

S

C

P

P

V

A

S

L

L

P

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

Q

L

I

S

G

L

I

I

V

D

D

F

N

D

D

N

V

V

V

D

E

D

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

V

L

H

H

R

D

E

S

N

S

N

R

I

V

G

G

M

M

A

L

K

K

V

C

R

E

S

S

A

A

E

R

E

Q

N

Y

L

I

K

T

A

K

L

L

N

N

H

P

F

H

V

I

D

N

I

V

F

V

A

T

Y

Y

D

P

Y

V

N

R

L

L

S

N

E

S

Q

S

E

N

F

A

E

F

A

D

I

I

I

F

E

K

K

G

A

Y

D

N

V

Y

V

I

I

L

D

D

C

C

T

T

D

D

N

S

F

P

S

L

T

T

R

R

F

Y

M

L

L

V

N

S

R

D

L

V

C

A

Y

V

A

N

Q

L

K

G

K

I

P

T

L

V

I

V

S

S

G

A

A

S

A

G

I

L

R

G

W

T

E

E

G

G

Q

Q

L

L

S

T

T

I

F

L

D

N

F

F

R

-

K

N

D

N

T

I

S

G

P

P

C

C

Y

Y

Q

R

C

C

L

F

Y

Y

K

P

E

T

D

P

S

P

G

P

Q

P

E

N

-

A

-

V

L

T

T

S

C
|
C

S

Q

E

E

N

G

G

G

V

V

L

I

A

G

P

P

V

C

V

I

G

G

M

L

V

V

G

G

S

T

I

M

Q

M

A

A

I

V

E

E

A

T

I

L

K

K

A

L

L

I

L

L

D

G

K

I

P

Y

T

T

-

N

-

E

-

N

L

F

T

S

G

P

K

F

L

L

M

M

I

L

L

Y

D

S

A

G

Y

F

T

P

-

Q

M

Q

N

S

I

L

R

R

T

T

L

F

N

K

L

M

K

R

-

G

K

R

D

Q

P

E

H

K

C

C

P

L

I

C

C

C

S

G

S

K

N

N

C225 (= C188) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
25% identity, 98% coverage: 5:249/250 of query aligns to 85:339/339 of 3h9jA

query
sites
3h9jA

N

N

D

S

D

T

E

E

L

N

S

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

L

L

L

H

S

Y

E

Q

V

-

D

S

Y

Y

D

G

G

A

-

N

-

P

-

V

-

L

-

V

Q

Q

L

D

K

K

L

L

T

K

Q

N

S

A

H

K

A

V

V

V

I

I

V

L

G

G

L

C

G
|
G

L

I

G

G

S

N

P

H

V

V

S

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

T

E

L

I

S

I

L

L

I

I

D
|
D

F

N

D
|
D

N

Q

V

I

D

E

D

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

V

L

H

F

R

S

E

E

N

D

I

V

G

G

M

K

A

N

K
|
K

V

T

R

E

S

V

A

I

E

K

E

R

N

E

L

L

K

L

A

K

L

R

N

N

H

S

F

E

V

I

D

S

I

V

F

S

A

E

Y

I

D

A

Y

L

N
|
N

L
x
I

S

N

E

D

Q

Y

E

T

F

D

E

L

A

H

I

K

I

V

E

P

K

E

A

A

D

D

V

I

V

W

I

V

D

V

C

S

T
x
A

D
|
D

N
x
H

-

P

F

F

S

N

T

L

R

I

F

N

M

W

L

V

N

N

R

K

L

Y

C

C

Y

V

A

R

Q

A

K

N

K

Q

P

P

L

Y

I

I

S

N

G

A

A

G

A

Y

I

V

R

-

W

-

E

-

G

N

Q

D

L

I

S

A

T

V

F

F

D

G

-

P

-

L

F

Y

R

V

K

P

D

G

T

K

S

T

P

G

C
|
C

Y

Y

Q

E

C

C

L

Q

Y

K

K

K

E

E

D

N

S

I

G

D

Q

H

E

K

L

I

T

K

C

L

S

I

E

N

N

S

-

R

-

F

-

K

-

P

G

A

V

T

L

F

A

A

P

P

V

V

V

N

G

N

M

V

V

A

G

A

S

A

I

L

Q

C

A

A

I

A

E

D

A

V

I

I

K

K

A

F

L

I

-

G

-

K

L

Y

D

S

K

E

P

P

T

L

L

S

T

L

G

N

K

K

L
x
R

M

I

I
x
G

L

I

D

W

A

S

Y

D

T

E

M

I

N

K

I

I

R

H

T

S

L

Q

N

N

L

M

K

G

K

R

D

S

P

P

H

V

C
|
C

P

S

I

V

C
|
C

S

G

S

N

active site: R157 (= R73)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G40), G125 (= G41), D146 (= D62), D148 (= D64), R157 (= R73), K170 (= K86), N193 (= N109), I194 (≠ L110), A213 (≠ T129), D214 (= D130), H215 (≠ N131)
binding zinc ion: C257 (= C173), C334 (= C244), C337 (= C247)
binding : R313 (≠ L223), G315 (≠ I225)

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
25% identity, 97% coverage: 5:247/250 of query aligns to 85:337/338 of 3h5nA

query
sites
3h5nA

N

N

D

S

D

T

E

E

L

N

S

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

L

L

L

H

S

Y

E

Q

V

-

D

S

Y

Y

D

G

G

A

-

N

-

P

-

V

-

L

-

V

Q

Q

L

D

K

K

L

L

T

K

Q

N

S

A

H

K

A

V

V

V

I

I

V

L

G

G

L

C

G
|
G

G

G

L

I

G

G

S

N

P

H

V

V

S

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

T

E

L

I

S

I

L

L

I

I

D
|
D

F

N

D

D

N

Q

V

I

D

E

D

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

I

V

V

L

H

F

R

S

E

E

N

D

I

V

G

G

M

K

A

N

K
|
K

V

T

R

E

S

V

A

I

E

K

E

R

N

E

L

L

K

L

A

K

L

R

N

N

H

S

F

E

V

I

D

S

I

V

F

S

A

E

Y

I

D

A

Y

L

N
|
N

L
x
I

S

N

E

D

Q

Y

E

T

F

D

E

L

A

H

I

K

I

V

E

P

K

E

A

A

D

D

V

I

V

W

I

V

D

V

C

S

T
x
A

D
|
D

N
x
H

-

P

F

F

S

N

T

L

R

I

F

N

M

W

L

V

N

N

R

K

L

Y

C

C

Y

V

A

R

Q

A

K

N

K

Q

P

P

L

Y

I

I

S

N

G

A

A

G

A

Y

I

V

R

-

W

-

E

-

G

N

Q

D

L

I

S

A

T

V

F

F

D

G

-

P

-

L

F

Y

R

V

K

P

D

G

T

K

S

T

P

G

C
|
C

Y

Y

Q

E

C
|
C

L

Q

Y

K

K

K

E

E

D

N

S

I

G

D

Q

H

E

K

L

I

T

K

C

L

S

I

E

N

N

S

-

R

-

F

-

K

-

P

G

A

V

T

L

F

A

A

P

P

V

V

V

N

G

N

M

V

V

A

G

A

S

A

I

L

Q

C

A

A

I

A

E

D

A

V

I

I

K

K

A

F

L

I

-

G

-

K

L

Y

D

S

K

E

P

P

T

L

L

S

T

L

G

N

K

K

L

R

M

I

I

G

L

I

D

W

A

S

Y

D

T

E

M

I

N

K

I

I

R

H

T

S

L

Q

N

N

L

M

K

G

K

R

D

S

P

P

H

V

C
|
C

P

S

I

V

C
|
C

active site: R157 (= R73)
binding adenosine-5'-triphosphate: G124 (= G40), D146 (= D62), N154 (= N70), R157 (= R73), Q158 (= Q74), K170 (= K86), N193 (= N109), I194 (≠ L110), A213 (≠ T129), D214 (= D130), H215 (≠ N131)
binding zinc ion: C257 (= C173), C260 (= C176), C334 (= C244), C337 (= C247)

3h5rA Crystal structure of e. Coli mccb + succinimide (see paper)
25% identity, 98% coverage: 5:249/250 of query aligns to 85:340/340 of 3h5rA

query
sites
3h5rA

N

N

D

S

D

T

E

E

L

N

S

N

R

R

Y

Y

S

S

R

R

Q

N

I

F

L

L

L

H

S

Y

E

Q

V

-

D

S

Y

Y

D

G

G

A

-

N

-

P

-

V

-

L

-

V

Q

Q

L

D

K

K

L

L

T

K

Q

N

S

A

H

K

A

V

V

V

I

I

V

L

G

G

L

C

G

G

L
x
I

G

G

S

N

P

H

V

V

S

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

T

E

L

I

S

I

L

L

I

I

D

D

F

N

D

D

N

Q

V

I

D

E

D

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

I

V

V

L

H

F

R

S

E

E

N

D

I

V

G

G

M

K

A

N

K

K

V

T

R

E

S

V

A

I

E

K

E

R

N

E

L

L

K

L

A

K

L

R

N

N

H

S

F

E

V

I

D

S

I

V

F

S

A

E

Y

I

D

A

Y

L

N

N

L

I

S

N

E

D

Q

Y

E

T

F

D

E

L

A

H

I

K

I

V

E

P

K

E

A

A

D

D

V

I

V

W

I

V

D

V

C
x
S

T
x
A

D

D

N

H

-

P

F

F

S

N

T

L

R

I

F

N

M

W

L

V

N

N

R

K

L

Y

C

C

Y

V

A

R

Q

A

K

N

K

Q

P

P

L

Y

I

I

S

N

G
x
A

A
x
G

A
x
Y

I

V

R

-

W

-

E

-

G

N

Q

D

L

I

S

A

T
x
V

F

F

D

G

-

P

-

L

F

Y

R

V

K

P

D

G

T

K

S

T

P

G

C
|
C

Y

Y

Q

E

C
|
C

L

Q

-

K

Y

V

K

K

E

E

D

N

S

I

G

D

Q

H

E

K

L

I

T

K

C

L

S

I

E

N

N

S

-

R

-

F

-

K

-
x
P

G

A

V

T

L

F

A

A

P

P

V

V

V

N

G

N

M

V

V

A

G

A

S

A

I

L

Q

C

A

A

I

A

E

D

A

V

I

I

K

K

A

F

L

I

-

G

-

K

L

Y

D

S

K

E

P

P

T

L

L

S

T

L

G

N

K

K

L
x
R

M

I