SitesBLAST
Comparing WP_029911765.1 NCBI__GCF_000711315.1:WP_029911765.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
64% identity, 97% coverage: 12:399/401 of query aligns to 4:390/392 of P33221
- EL 22:23 (= EL 30:31) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E90) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R122) binding ATP
- K155 (= K163) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 168:173) binding ATP
- G162 (= G170) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ D187) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFV 203:206) binding ATP
- E203 (= E211) binding ATP
- E267 (= E275) binding Mg(2+)
- E279 (= E287) binding Mg(2+)
- D286 (= D294) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K364) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 371:372) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
64% identity, 97% coverage: 12:399/401 of query aligns to 3:387/389 of 1kjiA
- active site: E114 (= E123), K154 (= K163), S159 (= S168), G161 (= G170), E264 (= E275), E276 (= E287), D283 (= D294), T284 (= T295), R360 (= R372)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R122), I152 (≠ V161), K154 (= K163), S159 (= S168), S160 (= S169), G161 (= G170), Q164 (= Q173), E192 (= E203), V195 (= V206), E200 (= E211), Q222 (= Q233), E264 (= E275), F266 (= F277), E276 (= E287)
- binding magnesium ion: E264 (= E275), E276 (= E287)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
64% identity, 97% coverage: 12:399/401 of query aligns to 3:387/389 of 1ez1A
- active site: E114 (= E123), K154 (= K163), S159 (= S168), G161 (= G170), E264 (= E275), E276 (= E287), D283 (= D294), T284 (= T295), R360 (= R372)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R122), I152 (≠ V161), K154 (= K163), S159 (= S168), S160 (= S169), G161 (= G170), E192 (= E203), V194 (≠ F205), V195 (= V206), F197 (= F208), E200 (= E211), Q222 (= Q233), E264 (= E275), F266 (= F277), E276 (= E287)
- binding glycinamide ribonucleotide: G20 (= G29), E21 (= E30), L22 (= L31), E81 (= E90), I82 (= I91), S160 (= S169), D283 (= D294), K352 (= K364), R359 (= R371), R360 (= R372)
- binding magnesium ion: E264 (= E275), E276 (= E287)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
64% identity, 97% coverage: 12:399/401 of query aligns to 3:387/389 of 1eyzA
- active site: E114 (= E123), K154 (= K163), S159 (= S168), G161 (= G170), E264 (= E275), E276 (= E287), D283 (= D294), T284 (= T295), R360 (= R372)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R122), I152 (≠ V161), K154 (= K163), S159 (= S168), S160 (= S169), G161 (= G170), Q164 (= Q173), E192 (= E203), V195 (= V206), F197 (= F208), E200 (= E211), E264 (= E275), F266 (= F277), E276 (= E287)
- binding magnesium ion: E264 (= E275), E276 (= E287)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
64% identity, 97% coverage: 12:399/401 of query aligns to 3:386/388 of 1kjqA
- active site: E114 (= E123), K154 (= K163), E263 (= E275), E275 (= E287), D282 (= D294), T283 (= T295), R359 (= R372)
- binding adenosine-5'-diphosphate: R113 (= R122), I152 (≠ V161), K154 (= K163), E191 (= E203), V193 (≠ F205), V194 (= V206), F196 (= F208), E199 (= E211), Q221 (= Q233), F265 (= F277), E275 (= E287)
- binding magnesium ion: E263 (= E275), E275 (= E287)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
64% identity, 97% coverage: 12:399/401 of query aligns to 3:384/386 of 1kjjA
- active site: E114 (= E123), K154 (= K163), S159 (= S168), G161 (= G170), E261 (= E275), E273 (= E287), D280 (= D294), T281 (= T295), R357 (= R372)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R122), I152 (≠ V161), K154 (= K163), S159 (= S168), S160 (= S169), G161 (= G170), Q164 (= Q173), E189 (= E203), V192 (= V206), E197 (= E211), Q219 (= Q233), E261 (= E275), F263 (= F277), E273 (= E287)
- binding magnesium ion: E261 (= E275), E273 (= E287)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
64% identity, 97% coverage: 12:399/401 of query aligns to 3:384/386 of 1kj8A
- active site: E114 (= E123), K154 (= K163), S159 (= S168), G161 (= G170), E261 (= E275), E273 (= E287), D280 (= D294), T281 (= T295), R357 (= R372)
- binding adenosine-5'-triphosphate: R113 (= R122), I152 (≠ V161), K154 (= K163), S159 (= S168), S160 (= S169), G161 (= G170), Q164 (= Q173), E189 (= E203), V192 (= V206), F194 (= F208), E197 (= E211), Q219 (= Q233), G222 (= G236), E261 (= E275), F263 (= F277), E273 (= E287)
- binding glycinamide ribonucleotide: G20 (= G29), E21 (= E30), L22 (= L31), E81 (= E90), I82 (= I91), S160 (= S169), D280 (= D294), K349 (= K364), R356 (= R371)
- binding magnesium ion: E261 (= E275), E273 (= E287)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
49% identity, 96% coverage: 12:397/401 of query aligns to 8:408/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
47% identity, 96% coverage: 12:397/401 of query aligns to 10:397/409 of 2dwcB
- active site: E265 (= E275), E277 (= E287), D284 (= D294), T285 (= T295), R372 (= R372)
- binding adenosine-5'-diphosphate: R120 (= R122), H159 (≠ V161), K161 (= K163), H190 (≠ F205), I191 (≠ V206), F193 (= F208), E196 (= E211), F267 (= F277), E277 (= E287)
4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
31% identity, 90% coverage: 30:391/401 of query aligns to 18:367/380 of 4dlkA
- active site: Y153 (vs. gap), G155 (= G170), E255 (= E275), E268 (= E287), N275 (≠ D294), S276 (≠ T295), K348 (≠ R372)
- binding adenosine-5'-triphosphate: E76 (= E90), F77 (≠ I91), R107 (= R122), K147 (= K163), Y153 (vs. gap), D154 (≠ S169), G155 (= G170), Q158 (= Q173), W184 (≠ F205), V185 (= V206), F187 (= F208), E190 (= E211), E255 (= E275), F257 (= F277), N267 (≠ S286), E268 (= E287), R272 (= R291), H274 (= H293), N275 (≠ D294), K340 (= K364), R347 (= R371), K348 (≠ R372)
- binding calcium ion: E255 (= E275), E268 (= E287)
- binding phosphate ion: Q47 (= Q59), A49 (= A61)
3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
31% identity, 90% coverage: 30:391/401 of query aligns to 17:366/380 of 3v4sA
- binding adenosine-5'-diphosphate: R106 (= R122), K146 (= K163), Y152 (vs. gap), G154 (= G170), Q157 (= Q173), W183 (≠ F205), V184 (= V206), E189 (= E211), N215 (≠ G236), F256 (= F277), N266 (≠ S286), E267 (= E287)
- binding carbonate ion: R271 (= R291), H273 (= H293), N274 (≠ D294)
3v4sB Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
31% identity, 90% coverage: 30:391/401 of query aligns to 19:368/381 of 3v4sB
- binding adenosine-5'-triphosphate: R108 (= R122), K148 (= K163), Y154 (vs. gap), D155 (≠ S169), G156 (= G170), Q159 (= Q173), E183 (= E203), W185 (≠ F205), V186 (= V206), F188 (= F208), E191 (= E211), H214 (≠ Q233), N217 (≠ G236), E256 (= E275), F258 (= F277), E269 (= E287)
- binding carbonate ion: R273 (= R291), H275 (= H293), N276 (≠ D294)
- binding magnesium ion: T105 (= T119), E111 (≠ R126), E256 (= E275), E269 (= E287), L270 (≠ V288)
3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
31% identity, 90% coverage: 30:391/401 of query aligns to 18:367/383 of 3r5hA
- binding adenosine-5'-diphosphate: R107 (= R122), K147 (= K163), Q158 (= Q173), W184 (≠ F205), V185 (= V206), F187 (= F208), E190 (= E211), N216 (≠ G236), F257 (= F277), N267 (≠ S286), E268 (= E287)
- binding 5-aminoimidazole ribonucleotide: Q18 (≠ E30), L19 (= L31), E76 (= E90), Y153 (vs. gap), R272 (= R291), K340 (= K364), R347 (= R371)
Sites not aligning to the query:
3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
31% identity, 90% coverage: 30:391/401 of query aligns to 18:367/377 of 3q2oB
3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
27% identity, 91% coverage: 35:400/401 of query aligns to 16:358/360 of 3ax6A
- active site: E231 (= E275), E244 (= E287), N251 (≠ D294), S252 (≠ T295), K330 (≠ R372)
- binding adenosine-5'-diphosphate: K101 (≠ R122), V136 (= V161), K138 (= K163), E164 (= E203), F166 (= F205), V167 (= V206), E172 (= E211), F233 (= F277), N243 (≠ S286)
4ma0A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with partially hydrolysed atp
29% identity, 74% coverage: 22:318/401 of query aligns to 2:290/366 of 4ma0A
- active site: Y144 (≠ S168), G146 (= G170), E247 (= E275), E259 (= E287), N266 (≠ D294), S267 (≠ T295)
- binding adenosine monophosphate: I136 (≠ V161), K138 (= K163), E175 (= E203), A176 (≠ G204), F177 (= F205), V178 (= V206), E183 (= E211), H206 (≠ Q233), F249 (= F277), E259 (= E287)
Sites not aligning to the query:
4mamA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an adp analog, amp-cp
29% identity, 74% coverage: 22:318/401 of query aligns to 2:290/373 of 4mamA
- active site: Y144 (≠ S168), G146 (= G170), E247 (= E275), E259 (= E287), N266 (≠ D294), S267 (≠ T295)
- binding phosphomethylphosphonic acid adenosyl ester: R98 (= R122), I136 (≠ V161), K138 (= K163), Y144 (≠ S168), G146 (= G170), Q149 (= Q173), E175 (= E203), F177 (= F205), V178 (= V206), F180 (= F208), E183 (= E211), H206 (≠ Q233), F249 (= F277), E259 (= E287)
Sites not aligning to the query:
5jqwA The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with adp
29% identity, 74% coverage: 22:318/401 of query aligns to 2:290/365 of 5jqwA
- active site: Y144 (≠ S168), G146 (= G170), E247 (= E275), E259 (= E287), N266 (≠ D294), S267 (≠ T295)
- binding adenosine-5'-diphosphate: R98 (= R122), K138 (= K163), G143 (≠ S167), Y144 (≠ S168), D145 (≠ S169), G146 (= G170), V178 (= V206), E183 (= E211), H206 (≠ Q233), F249 (= F277), E259 (= E287)
Sites not aligning to the query:
3aw8A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermus thermophilus hb8
28% identity, 90% coverage: 33:392/401 of query aligns to 12:352/360 of 3aw8A
- active site: E240 (= E275), E252 (= E287), N259 (≠ D294), S260 (≠ T295), K329 (≠ R371)
- binding adenosine monophosphate: L135 (≠ V161), K137 (= K163), Q142 (= Q173), F168 (= F205), V169 (= V206), E174 (= E211), H197 (≠ Q233), F242 (= F277), E252 (= E287)
4ma5A The crystal structure of phosphoribosylaminoimidazole carboxylase atpase subunit of francisella tularensis subsp. Tularensis schu s4 in complex with an atp analog, amp-pnp.
29% identity, 74% coverage: 22:318/401 of query aligns to 2:288/363 of 4ma5A
- active site: Y144 (≠ S168), G146 (= G170), E247 (= E275), E257 (= E287), N264 (≠ D294), S265 (≠ T295)
- binding phosphoaminophosphonic acid-adenylate ester: I136 (≠ V161), K138 (= K163), D145 (≠ S169), G146 (= G170), F177 (= F205), V178 (= V206), E183 (= E211), H206 (≠ Q233), E247 (= E275), F249 (= F277), N256 (≠ S286), E257 (= E287), H263 (= H293)
Sites not aligning to the query:
Query Sequence
>WP_029911765.1 NCBI__GCF_000711315.1:WP_029911765.1
MSQDSATNTVTIGTKFSPTATKLLLCGSGELGKEVALEAQRFGIEVIALDRYENAPAMQV
ADKSYTLSMLDGDALRDIILKEKPDFIVPEIEAIATDTLAELEQQGFNVVPTAKATQLTM
NREGIRRLAAETLGLKTSPYAFADNKEDFVQAVNNIGIPCVVKPIMSSSGKGQSVIKTAA
DIDKAWDYAQEGGRAGKGKVIVEGFVDFDYEITLLTVRHIDGTSFCEPIGHLQEDGDYRQ
SWQPHPMTSTALEKAQQMAKSITDALGGRGLFGVELFVKGDEVIFSEVSPRPHDTGLVTL
ISQDISEFALHVRAILGLPIPNIVQHGPSASSVILPTGQSTQTAFGNLYQALSEPDTQIK
LFGKPEIAGRRRMGVALARGNSIEEALEKAKRASQAVTVTF
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory