SitesBLAST
Comparing WP_029913199.1 NCBI__GCF_000711315.1:WP_029913199.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
25% identity, 52% coverage: 15:501/938 of query aligns to 39:460/467 of 7qh2C
- binding flavin-adenine dinucleotide: V73 (≠ M50), G75 (≠ A52), S76 (≠ G53), G77 (= G54), T78 (= T55), G79 (≠ S56), L80 (= L57), A83 (≠ Q60), C84 (≠ A61), P137 (≠ T113), G138 (≠ S114), E139 (≠ T115), A142 (= A119), T143 (≠ M120), G146 (= G123), N147 (≠ M124), S149 (≠ G126), T150 (≠ N127), A152 (≠ S129), G153 (≠ C130), E203 (= E240), G204 (= G241), I209 (≠ V246), E422 (= E463), H423 (= H464)
- binding fe (iii) ion: H377 (= H419), H384 (= H426), E422 (= E463)
P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
25% identity, 54% coverage: 2:507/938 of query aligns to 36:458/459 of P9WIT1
- K354 (≠ D398) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
25% identity, 47% coverage: 61:505/938 of query aligns to 81:455/455 of 8jdeA
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), W322 (≠ L370), E413 (= E463), H414 (= H464), N450 (= N500)
- binding lactic acid: R318 (= R366), H369 (= H419), H376 (= H426), H414 (= H464)
- binding manganese (ii) ion: H369 (= H419), H376 (= H426), E413 (= E463)
Sites not aligning to the query:
8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
25% identity, 47% coverage: 61:505/938 of query aligns to 81:455/455 of 8jduA
- binding 2-oxopentanoic acid: R318 (= R366), W322 (≠ L370), H369 (= H419), H376 (= H426), H414 (= H464)
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), W322 (≠ L370), E413 (= E463), N450 (= N500)
- binding manganese (ii) ion: H369 (= H419), H376 (= H426), E413 (= E463)
Sites not aligning to the query:
8jdzA Crystal structure of mldhd in complex with 2-keto-3-methylvaleric acid (see paper)
25% identity, 47% coverage: 61:505/938 of query aligns to 81:454/454 of 8jdzA
- binding (3S)-3-methyl-2-oxopentanoic acid: R318 (= R366), W322 (≠ L370), H369 (= H419), H376 (= H426), H413 (= H464)
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), W322 (≠ L370), E412 (= E463), H413 (= H464), N449 (= N500)
- binding manganese (ii) ion: H369 (= H419), H376 (= H426), E412 (= E463)
Sites not aligning to the query:
8jdyA Crystal structure of mldhd in complex with 2-ketoisocaproic acid (see paper)
25% identity, 47% coverage: 61:505/938 of query aligns to 81:454/454 of 8jdyA
- binding 2-oxo-4-methylpentanoic acid: R318 (= R366), W322 (≠ L370), S336 (≠ A384), H369 (= H419), H376 (= H426), H413 (= H464)
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), E412 (= E463), N449 (= N500)
- binding manganese (ii) ion: H369 (= H419), H376 (= H426), E412 (= E463)
Sites not aligning to the query:
8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
25% identity, 47% coverage: 61:505/938 of query aligns to 81:454/454 of 8jdvA
- binding 2-Ketohexanoic acid: R317 (= R366), W321 (≠ L370), H368 (= H419), H375 (= H426), H413 (= H464)
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), W321 (≠ L370), Y322 (≠ G371), E412 (= E463), H413 (= H464), N449 (= N500)
- binding manganese (ii) ion: H368 (= H419), H375 (= H426), E412 (= E463)
Sites not aligning to the query:
8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
24% identity, 47% coverage: 61:505/938 of query aligns to 81:456/456 of 8jdsA
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), W323 (≠ L370), E414 (= E463), H415 (= H464), N451 (= N500)
- binding manganese (ii) ion: H370 (= H419), H377 (= H426), E414 (= E463)
- binding pyruvic acid: R319 (= R366), H370 (= H419), H377 (= H426), H415 (= H464)
Sites not aligning to the query:
8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
25% identity, 47% coverage: 61:505/938 of query aligns to 81:455/455 of 8jdxA
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), W322 (≠ L370), E413 (= E463), H414 (= H464), N450 (= N500)
- binding 3-methyl-2-oxobutanoic acid: R318 (= R366), H369 (= H419), H376 (= H426), H414 (= H464)
- binding manganese (ii) ion: H369 (= H419), H376 (= H426), E413 (= E463)
Sites not aligning to the query:
8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
24% identity, 47% coverage: 61:505/938 of query aligns to 81:455/455 of 8jdtA
- binding 2-ketobutyric acid: R318 (= R366), H369 (= H419), H376 (= H426), H414 (= H464)
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), W322 (≠ L370), E413 (= E463), H414 (= H464), N450 (= N500)
- binding manganese (ii) ion: H369 (= H419), H376 (= H426), E413 (= E463)
Sites not aligning to the query:
8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
24% identity, 47% coverage: 61:505/938 of query aligns to 81:456/456 of 8jdrA
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), Y324 (≠ G371), H370 (= H419), E414 (= E463), N451 (= N500)
- binding (2R,3S)-3-methyl-2-oxidanyl-pentanoic acid: R319 (= R366), W323 (≠ L370), H415 (= H464)
Sites not aligning to the query:
8jdqA Crystal structure of h405a mldhd in complex with d-2-hydroxyisocaproic acid (see paper)
24% identity, 47% coverage: 61:505/938 of query aligns to 81:456/456 of 8jdqA
- binding (2R)-2-hydroxy-4-methylpentanoic acid: R319 (= R366), W323 (≠ L370), H370 (= H419), H415 (= H464)
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), H370 (= H419), E414 (= E463), N451 (= N500)
Sites not aligning to the query:
8jdoA Crystal structure of h405a mldhd in complex with d-2-hydroxyhexanoic acid (see paper)
24% identity, 47% coverage: 61:505/938 of query aligns to 81:456/456 of 8jdoA
- binding (2R)-2-hydroxyhexanoic acid: R319 (= R366), W323 (≠ L370), H415 (= H464)
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), Y324 (≠ G371), H370 (= H419), E414 (= E463), N451 (= N500)
Sites not aligning to the query:
8jdnA Crystal structure of h405a mldhd in complex with d-2-hydroxyvaleric acid (see paper)
24% identity, 47% coverage: 61:505/938 of query aligns to 81:456/456 of 8jdnA
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), H370 (= H419), E414 (= E463), N451 (= N500)
- binding (2R)-2-oxidanylpentanoic acid: R319 (= R366), W323 (≠ L370), H415 (= H464)
Sites not aligning to the query:
8jdgA Crystal structure of h405a mldhd in complex with d-2-hydroxybutanoic acid (see paper)
24% identity, 47% coverage: 61:505/938 of query aligns to 81:456/456 of 8jdgA
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), H370 (= H419), E414 (= E463), N451 (= N500)
- binding (2R)-2-oxidanylbutanoic acid: R319 (= R366), H415 (= H464)
Sites not aligning to the query:
8jdbA Crystal structure of h405a mldhd in complex with d-2-hydroxyoctanoic acid (see paper)
24% identity, 47% coverage: 61:505/938 of query aligns to 81:456/456 of 8jdbA
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), Y324 (≠ G371), H370 (= H419), E414 (= E463), N451 (= N500)
- binding (2R)-2-oxidanyloctanoic acid: R319 (= R366), W323 (≠ L370), H415 (= H464)
Sites not aligning to the query:
8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
25% identity, 47% coverage: 61:505/938 of query aligns to 81:455/455 of 8jdpA
- binding flavin-adenine dinucleotide: L90 (≠ V70), P132 (≠ T113), G133 (≠ S114), A134 (≠ T115), G140 (= G123), M141 (= M124), A143 (≠ G126), T144 (≠ N127), A146 (≠ S129), S147 (≠ C130), E200 (= E240), G201 (= G241), I206 (≠ V246), H369 (= H419), E413 (= E463), N450 (= N500)
- binding deaminohydroxyvaline: R319 (= R366), H414 (= H464)
Sites not aligning to the query:
3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
22% identity, 51% coverage: 28:505/938 of query aligns to 47:465/465 of 3pm9A
- active site: A149 (≠ S129), L159 (≠ T139)
- binding flavin-adenine dinucleotide: P69 (≠ M50), Q70 (≠ R51), G71 (≠ A52), G72 (= G53), N73 (≠ G54), T74 (= T55), G75 (≠ S56), L76 (= L57), G79 (= G59), Q80 (= Q60), L91 (≠ V70), L133 (≠ T113), G134 (≠ S114), A135 (≠ T115), C139 (≠ A119), T140 (≠ M120), G142 (= G122), G143 (= G123), S146 (≠ G126), T147 (≠ N127), A149 (≠ S129), G150 (≠ C130), E200 (= E240), G201 (= G241), I205 (≠ V245), I206 (≠ V246), E423 (= E463)
P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
21% identity, 51% coverage: 23:501/938 of query aligns to 69:492/496 of P39976
Sites not aligning to the query:
- 17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
21% identity, 51% coverage: 28:501/938 of query aligns to 106:513/521 of Q8N465
- S109 (≠ L31) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
- N127 (≠ T49) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
- G131 (= G53) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
- I147 (≠ L67) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
- M153 (≠ L74) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
- C172 (≠ V93) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
- P189 (≠ A110) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
- A205 (≠ G126) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
- A231 (≠ S152) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
- G233 (= G154) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
- D375 (= D355) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
- R386 (= R366) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- T390 (≠ M374) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
- V399 (= V383) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
- K401 (≠ V385) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- R419 (≠ E404) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
- A426 (≠ G411) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
- H434 (= H419) binding Zn(2+); mutation to A: Loss of catalytic activity.
- G436 (≠ S421) to V: slight reduction in catalytic activity
- N439 (≠ L424) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
- H441 (= H426) binding Zn(2+); mutation to A: Loss of catalytic activity.
- N443 (≠ D432) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
- V444 (≠ L433) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
- A446 (≠ T435) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
- E475 (= E463) binding Zn(2+); mutation to A: Loss of catalytic activity.
- H476 (= H464) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
- G477 (= G465) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640
Sites not aligning to the query:
- 15 R → G: in dbSNP:rs4675887
- 169:521 natural variant: Missing (in D2HGA1; uncertain significance)
- 400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)
Query Sequence
>WP_029913199.1 NCBI__GCF_000711315.1:WP_029913199.1
MPPQNNSFSKSATDASVYEFPLNKWVTPRSLPELVKTCREAFSNKQSVTMRAGGTSLGGQ
AIGSEVLIDVSKHLTQILDYRPERKEVVVEPGVIQDDLNAYVKANGLRFAPDTSTSNRAM
IGGMIGNNSCGSYSVYYGTTREHVKSVEVILSDGSEVIFEDLTTEELAHKQTLDTLEGHL
YRGVMSMLEEHGDTILENFPDASIKRRNTGYALDELYRHHQPFNPHGKPFNLTPLICGSE
GTLAVVKSARLGLVEAPKHRQLICAHYHSVREAMQVVPEFLQFHPAAIELIDRPTLEGTK
DNKALQPNRFWIQDDPAAVLVVELFDDCLDSLNARLRICQAWLAEKGSYATPIIDAADSD
KVWSIRKAGLGLLMGKITRQKAVAVIEDAAVPVAKLPDYYHDVEQMMAELGVGCIYYGHA
SVGLIHIRPELDLATEKGRQLFEEIAKRNSKLVKSYRGAISGEHGDGRIRAPFIKEQVGE
TVYQCLVNLKRLFDPQNLLNPGVIIGDMPITQNLRAARQPQATLTTGFDWSNDLSLMDAV
EKCNGAAACRKSTGTMCPSYQATREENYSTRGRSNLLRFALTEPNPQTALSNAELQDALE
MCLGCKACHSECPANVDMARLKAEVLYQTGKMSLSRLALKYYGFLMRVGSVFPRIYNQVQ
NLGWVKRLMNVDSRRALPTLSNLELSRWWQTTDNASKDNCQRVWLLCDLYSRYQESEVGQ
AAILTLHKLGCDVQPIFLEASPRALISQGLLDGARKALLDINRQLSAVQENDWIVGIEPS
EVLVWRDEAQALMKAETQTWQILLFEEAVLKLAEKGELPFQALTRKVKVHTHCHQKSLAK
EADVAKALSLIPALEVERIASGCCGMSGEFGYRHYDVSKKIAEQSLLPAIEEGDALIVAT
GTSCRHQLDDFSSKQGLHSAQVFLQALQDIEWSSAGRR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory