SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_029933551.1 NCBI__GCF_000711195.1:WP_029933551.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
63% identity, 99% coverage: 7:1233/1239 of query aligns to 4:1225/1227 of P13009

query
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P13009

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C247 (= C249) binding Zn(2+)
C310 (= C312) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
C311 (= C313) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
E694 (= E694) binding methylcob(III)alamin
GDVHD 756:760 (= GDVHD 756:760) binding methylcob(III)alamin
D757 (= D757) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
H759 (= H759) binding axial binding residue; mutation to G: Loss of catalytic activity.
S804 (= S804) binding methylcob(III)alamin
T808 (= T808) binding methylcob(III)alamin
S810 (= S810) mutation to A: Decreases activity by about 40%.
A860 (= A860) binding methylcob(III)alamin
D946 (= D947) binding S-adenosyl-L-methionine
R1134 (= R1142) binding S-adenosyl-L-methionine
YY 1189:1190 (≠ YF 1197:1198) binding S-adenosyl-L-methionine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
53% identity, 100% coverage: 1:1233/1239 of query aligns to 1:1263/1265 of Q99707

query
sites
Q99707

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D

R61 (≠ M50) natural variant: R -> K
C255 (≠ S244) to Y: in dbSNP:rs1140598
GSR 382:384 (≠ GSA 369:371) binding (6S)-5,6,7,8-tetrahydrofolate
D449 (= D436) binding (6S)-5,6,7,8-tetrahydrofolate
N470 (= N457) binding (6S)-5,6,7,8-tetrahydrofolate
D537 (= D523) binding (6S)-5,6,7,8-tetrahydrofolate
N579 (= N565) binding (6S)-5,6,7,8-tetrahydrofolate
R585 (= R571) binding (6S)-5,6,7,8-tetrahydrofolate
R591 (= R577) binding (6S)-5,6,7,8-tetrahydrofolate
D919 (≠ E893) to G: in dbSNP:rs1805087
D963 (= D936) mutation to E: Decreases binding to MTRR; when associated with N-1071.
K1071 (= K1036) mutation to N: Decreases binding to MTRR; when associated with E-963.

3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
60% identity, 47% coverage: 654:1233/1239 of query aligns to 4:575/576 of 3ivaA

query
sites
3ivaA

W

W

R

R

E

S

A

W

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E

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V

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N

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L

C

Q

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

D

D

G

G

M

M

N

N

V

V

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

R

Q

A

A

V

V

A

A

Y

Y

L

L

Q

E

P

P

Y

F

L

I

E

E

A

A

E

S

K

K

A

E

S

Q

G

C

Q

K

A

T

K

N

G

G

K

K

I

M

V

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G
|
G

K

K

N

N

I

I

V
|
V

G

G

V

V

V

V

L

L

Q

Q

C

C

N

N

N

N

F

Y

D

E

V

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

T

A

E

E

K

K

I

I

L

L

D

R

T

T

A

A

I

K

K

E

E

V

N

N

A

A

N

D

V

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

E

D

E

E

M

M

V

V

N

N

V

V

A

A

K

K

E

E

M

M

Q

E

R

R

R

Q

G

G

M

F

N

T

L

I

P

P

L

L

L

L

I

I

G
|
G

G
|
G

A

A

T

T

T

T

S

S

K

K

A

A

H

H

T

T

A

A

V

V

K

K

I

I

E

E

P

Q

Q

N

Y

Y

D

S

H

G

P

P

V

T

V

V

Y

Y

V

V

K
x
Q

D
x
N

A

A

S

S

R

R

A

T

V

V

G

G

V

V

A

V

Q

A

S

A

L

L

I

L

S

S

N

D

D

T

L

Q

K

R

V

D

D

D

Y

F

A

V

A

A

K

R

I

T

R

R

A

K

E

E

Y

Y

E

E

Q

T

L

V

R

R

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I

E

Q

R

H

K

G

A

R

R

K

S

K

K

P

Q

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T

K

P

R

P

T

V

P

T

L

L

N

E

K

A

A

A

R

R

E

D

N

N

P

D

I

F

P

A

V

F

D

D

W

W

T

Q

N

A

Y

Y

V

T

P

P

P

P

K

V

P

A

S

H

F

R

L

L

G

G

T

V

K

Q

V

E

F

V

D

-

D

E

I

A

P

S

L

I

T

E

E

T

L

L

L

R

D

N

R

Y

L

I

D

D

W

W

S

T

P

P

F

F

F

F

Q

M

S
x
T

W

W

D

S

L

L

H

A

G

G

L

K

Y

Y

P

P

R

R

I

I

L

L

D

E

D

D

K

E

L

V

V

V

G

G

E

V

E

E

A

A

R

Q

K

R

V

L

F

F

A

K

D

D

A

A

K

N

T

D

M

M

L

L

Q

D

T

K

I

L

I

S

K

A

E

E

K

K

W

T

L

L

T

N

A

P

R

R

A

G

V

V

I

V

G

G

F

L

Y

F

P

P

A

A

N

N

S

R

V

V

V

G

D

D

D

D

I

I

E

E

L

I

Y

Y

K

R

D

D

D

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S

T

R

R

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T

E

H

V

V

L

I

T

N

R

V

L

S

H

H

N

H

L

L

R

R

Q

Q

Q

Q

A

T

D

E

K

-

K

K

A

T

G

G

Q

F

Y

A

N

N

Q

Y

C

C

L

L

S

A

D

D

Y

F

I

V

A

A

P

P

K

K

A

L

N

S

K

G

Q

K

Q

A

P

-

G

-

L

-

V

-

L

-

E

-

D

D

Y

Y

I

I

G

G

A

A

F

F

A

A

V

V

T

T

A

G

G

G

I

L

G

E

I

E

D

D

E

A

H

L

I

A

A

D

R

A

F

F

E

E

A

A

A

Q

H

H

D

D

D

D

Y

Y

Q

N

A

K

I

I

M

M

L

V

K

K

A

A

L

L

A

A

D
|
D

R

R

L

L

A

A

E
|
E

A

A

L

F

A

A

E

E

Y

Y

M

L

H

H

E

E

K

R

V

V

R

R

K

K

E

V

F

Y

W

W

G

G

Y

Y

A

A

A

P

D

N

E

E

Q

N

L

L

T

S

N

N

E

E

E

E

M

L

I

I

K

R

E

E

K

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G
|
G

Y
|
Y

P

P

A
|
A

N

C

P

P

E

E

H
|
H

T

T

E

E

K

K

G

A

T

T

L

I

W

W

S

E

L

L

L

L

K

E

P

V

D

E

S

K

E

H

I

T

G

G

L

M

E

K

L

L

T

T

S

E

S

S

Y

F

A
|
A

M
|
M

T

W

P

P

T
x
G

A

A

A

S

V
|
V

S
|
S

G

G

W

W

Y

Y

F

F

A

S

H

H

P

P

E

D

S

S

K

K

Y
|
Y

F

Y

G

A

V

V

G

A

S

Q

I

I

G

Q

R

R

D

D

Q

Q

A

V

E

E

D

D

Y

Y

A

A

K

R

R

R

K

K

N

G

W

M

T

S

I

V

Q

T

E

E

A

V

E

E

K

R

W

W

L

L

A

A

P

P

N

N

L

L

G

G

Y

Y

D

D

active site: D107 (= D757), H109 (= H759), S160 (= S810)
binding cobalamin: H109 (= H759), G112 (= G762), V116 (= V766), G152 (= G802), L153 (= L803), S154 (= S804), L156 (= L806), I157 (= I807), T158 (= T808), G183 (= G833), G184 (= G834), Q208 (≠ K858), N209 (≠ D859), T303 (≠ S954), D443 (= D1101), A486 (= A1144), G488 (= G1146), Y489 (= Y1147), H495 (= H1153), A520 (= A1178), M521 (= M1179), G524 (≠ T1182), V527 (= V1185), S528 (= S1186)
binding s-adenosyl-l-homocysteine: E447 (= E1105), R484 (= R1142), P485 (= P1143), Y489 (= Y1147), A491 (= A1149), Y539 (= Y1197)

3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
60% identity, 47% coverage: 654:1233/1239 of query aligns to 4:575/577 of 3bulA

query
sites
3bulA

W

W

R

R

E

S

A

W

S

E

V

V

E

N

K

K

R

R

L

L

E

E

H

Y

S

S

L

L

V

V

K

K

G

G

I

I

T

T

D

E

Y

F

I

I

E

E

D

Q

D

D

T

T

E

E

E

E

A

A

R

R

T

Q

T

Q

L

A

G

T

S

R

P

P

L

C

Q

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

D

D

G

G

M

M

N

N

V

V

V

V

G

G

D

D

L

L

F

F

G

G

A

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

R

Q

A

A

V

V

A

A

Y

Y

L

L

Q

E

P

P

Y

F

L

I

E

E

A

A

E

S

K

K

A

E

S

Q

G

C

Q

K

A

T

K

N

G

G

K

K

I

M

V

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G

G

K

K

N

N

I

I

V
|
V

G

G

V

V

V

V

L

L

Q

Q

C

C

N

N

N

N

F

Y

D

E

V

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

T

A

E

E

K

K

I

I

L

L

D

R

T

T

A

A

I

K

K

E

E

V

N

N

A

A

N

D

V

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

E

D

E

E

M

M

V

V

N

N

V

V

A

A

K

K

E

E

M

M

Q

E

R

R

R

Q

G

G

M

F

N

T

L

I

P

P

L

L

L

L

I

I

G
|
G

G
|
G

A

A

T

T

T

T

S

S

K

K

A

A

H

H

T

T

A

A

V

V

K

K

I

I

E

E

P

Q

Q

N

Y

Y

D

S

H

G

P

P

V

T

V

V

Y

Y

V

V

K
x
Q

D
x
N

A
|
A

S

S

R

R

A
x
T

V

V

G

G

V

V

A

V

Q

A

S

A

L

L

I

L

S

S

N

D

D

T

L

Q

K

R

V

D

D

D

Y

F

A

V

A

A

K

R

I

T

R

R

A

K

E

E

Y

Y

E

E

Q

T

L

V

R

R

E

I

E

Q

R

H

K

G

A

R

R

K

S

K

K

P

Q

R

V

T

K

P

R

P

T

V

P

T

L

L

N

E

K

A

A

A

R

R

E

D

N

N

P

D

I

F

P

A

V

F

D

D

W

W

T

Q

N

A

Y

Y

V

T

P

P

P

P

K

V

P

A

S

H

F

R

L

L

G

G

T

V

K

Q

V

E

F

V

D

-

D

E

I

A

P

S

L

I

T

E

E

T

L

L

L

R

D

N

R

Y

L

I

D

D

W

W

S

T

P

P

F

F

F

F

Q
x
M

S

T

W

W

D

S

L

L

H

A

G

G

L

K

Y

Y

P

P

R

R

I

I

L

L

D

E

D

D

K

E

L

V

V

V

G

G

E

V

E

E

A

A

R

Q

K

R

V

L

F

F

A

K

D

D

A

A

K

N

T

D

M

M

L

L

Q

D

T

K

I

L

I

S

K

A

E

E

K

K

W

T

L

L

T

N

A

P

R

R

A

G

V

V

I

V

G

G

F

L

Y

F

P

P

A

A

N

N

S

R

V

V

V

G

D

D

D

D

I

I

E

E

L

I

Y

Y

K

R

D

D

D

E

S

T

R

R

E

T

E

H

V

V

L

I

T

N

R

V

L

S

H

H

N

H

L

L

R

R

Q

Q

Q

Q

A

T

D

E

K

-

K

K

A

T

G

G

Q

F

Y

A

N

N

Q

Y

C

C

L

L

S

A

D

D

Y

F

I

V

A

A

P

P

K

K

A

L

N

S

K

G

Q

K

Q

A

P

-

G

-

L

-

V

-

L

-

E

-

D

D

Y

Y

I

I

G

G

A

A

F

F

A

A

V

V

T

T

A

G

G

G

I

L

G

E

I

E

D

D

E

A

H

L

I

A

A

D

R

A

F

F

E

E

A

A

A

Q

H

H

D

D

D

D

Y

Y

Q

N

A

K

I

I

M

M

L

V

K

K

A

A

L

L

A

A

D
|
D

R

R

L

L

A

A

E

E

A

A

L

F

A

A

E

E

Y

Y

M

L

H

H

E

E

K

R

V

V

R

R

K

K

E

V

F

Y

W

W

G

G

Y

Y

A

A

A

P

D

N

E

E

Q

N

L

L

T

S

N

N

E

E

E

E

M

L

I

I

K

R

E

E

K

N

Y

Y

R

Q

G

G

I

I

R

R

P

P

A
|
A

P
|
P

G
|
G

Y
|
Y

P

P

A

A

N

C

P

P

E

E

H
|
H

T

T

E

E

K
|
K

G

A

T

T

L

I

W

W

S

E

L

L

L

L

K

E

P

V

D

E

S

K

E

H

I

T

G

G

L

M

E

K

L

L

T

T

S

E

S

S

Y

F

A

A

M
|
M

T

W

P

P

T
x
G

A

A

A

S

V
|
V

S
|
S

G

G

W

W

Y

Y

F

F

A

S

H

H

P

P

E

D

S

S

K

K

Y

Y

F

Y

G

A

V

V

G

A

S

Q

I

I

G

Q

R

R

D

D

Q

Q

A

V

E

E

D

D

Y

Y

A

A

K

R

R

R

K

K

N

G

W

M

T

S

I

V

Q

T

E

E

A

V

E

E

K

R

W

W

L

L

A

A

P

P

N

N

L

L

G

G

Y

Y

D

D

active site: D107 (= D757), H109 (= H759), S160 (= S810)
binding cobalamin: H109 (= H759), V116 (= V766), G152 (= G802), L153 (= L803), S154 (= S804), L156 (= L806), I157 (= I807), T158 (= T808), G183 (= G833), G184 (= G834), Q208 (≠ K858), N209 (≠ D859), A210 (= A860), T213 (≠ A863), M302 (≠ Q953), D443 (= D1101), A486 (= A1144), P487 (= P1145), G488 (= G1146), Y489 (= Y1147), H495 (= H1153), K498 (= K1156), M521 (= M1179), G524 (≠ T1182), V527 (= V1185), S528 (= S1186)

4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
53% identity, 51% coverage: 10:637/1239 of query aligns to 5:611/611 of 4cczA

query
sites
4cczA

Q

E

L

I

K

N

S

A

L

I

L

L

T

Q

E

K

R

R

V

I

V

M

V

V

L

L

D

D

G

G

A

G

M

M

G

G

T

T

M

M

I

I

Q

Q

N

R

L

E

Q

K

L

L

S

-

E

E

D

E

D

H

F

F

R

R

G

G

A

Q

R

E

F

F

A

K

D

D

Y

H

H

A

M

R

D

P

I

L

K

K

G

G

N

N

N

N

D

D

I

I

L

L

V

S

L

I

T

T

Q

Q

P

P

Q

D

L

V

I

I

R

Y

D

Q

I

I

H

H

L

K

S

E

F

Y

L

L

R

L

Q

-

G

-

V

A

D

D

L

I

L

I

E

E

T

T

N

N

S

T

F

F

N

S

A

S

T

T

T

S

I

I

A

A

Q

Q

A

A

D

D

Y

Y

D

G

M

L

E

E

S

H

Q

L

V

A

R

Y

D

R

I

M

N

N

L

M

H

C

A

S

A

A

Q

G

V

V

A

A

R

R

E

K

A

A

C

A

E

E

I

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|
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|
G

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A

A

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Q

binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E363), G342 (= G369), R344 (≠ A371), N430 (= N457), M458 (= M485), D497 (= D523), G536 (= G562), S538 (= S564), N539 (= N565), F542 (= F568), R545 (= R571), R551 (= R577)

8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
35% identity, 69% coverage: 15:873/1239 of query aligns to 9:835/841 of 8g3hA

query
sites
8g3hA

L

L

T

S

E

Q

R

R

V

V

V

L

V

V

L

F

D

D

G

G

A

A

M

M

G

G

T

T

M

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I

L

Q

Q

N

K

L

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D

L

L

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D

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D

D

F

Y

R

-

G

-

A

G

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F

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A

A

D

Y

Y

Y

H

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M

-

D

-

I

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K

-

G

G

N

C

N

P

D

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I

V

L

L

V

N

L

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T

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P

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G

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N

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F

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N

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D

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F

F

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A

A

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D

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A

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A

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L

N

A

A

A

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L

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S

A

L

K

P

P

I

L

D

S

V

I

V

G

G

L

M

N

N

C

C

A

A

L

T

G

G

P

P

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D

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L

L

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V

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A

V

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L

P

P

N

N

A

A

G

G

L

L

P

P

-

R

N

N

E

E

F

G

G

G

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R