SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_029934730.1 NCBI__GCF_000711195.1:WP_029934730.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
31% identity, 96% coverage: 11:362/367 of query aligns to 7:376/386 of P0A9J8

query
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P0A9J8

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R11 (= R15) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R32) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (≠ E50) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E59) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (≠ E94) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

2qmxA The crystal structure of l-phe inhibited prephenate dehydratase from chlorobium tepidum tls (see paper)
33% identity, 72% coverage: 99:363/367 of query aligns to 4:275/278 of 2qmxA

query
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2qmxA

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active site: T169 (= T266), F171 (= F268)
binding phenylalanine: N204 (= N292), E205 (≠ R293), L209 (= L297), D222 (≠ G310), L223 (≠ M311), T224 (= T312), K225 (≠ R313), S228 (= S316), Y238 (= Y326), F240 (= F328)

3mwbA The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
32% identity, 72% coverage: 97:361/367 of query aligns to 1:274/306 of 3mwbA

query
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3mwbA

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active site: T176 (= T266), F178 (= F268)
binding phenylalanine: D205 (≠ N292), H206 (≠ R293), L210 (= L297), N223 (≠ G310), L224 (≠ M311), S225 (≠ T312), R226 (= R313), S229 (= S316), F241 (= F328)

3mwbB The crystal structure of prephenate dehydratase in complex with l-phe from arthrobacter aurescens to 2.0a
32% identity, 72% coverage: 97:361/367 of query aligns to 1:271/303 of 3mwbB

query
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3mwbB

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active site: T176 (= T266), F178 (= F268)
binding magnesium ion: A155 (= A241), Q158 (= Q244), L161 (= L251)
binding phenylalanine: D205 (≠ N292), H206 (≠ R293), L210 (= L297), N223 (≠ G310), L224 (≠ M311), S225 (≠ T312), R226 (= R313), I227 (= I314), Y236 (= Y326), F238 (= F328)

6vh5D Crystal structure of prephenate dehydratase from brucella melitensis biovar abortus 2308 in complex with phenylalanine
32% identity, 72% coverage: 98:361/367 of query aligns to 8:276/282 of 6vh5D

query
sites
6vh5D

K

R

V

I

A

S

Y

F

L

Q

G

G

P

E

E

A

G

G

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A

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S

H

D

A

T

A

A

T

C

I

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Q

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F

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D

F

M

A

-

Q

E

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P

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C

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T

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F

E

E

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D

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A

F

F

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N

S

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G

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A

T

A

H

D

Y

L

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A

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P

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I

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E

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N

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E

A

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A

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L

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A

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H

H

A

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H

A

A

L

L

G

G

Q

Q

C

C

R

R

S

N

W

V

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I

K

R

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Q

N

N

L

-

P

-

G

G

V

W

E

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L

G

E

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A

I

V

A

E

G

S

D

N

T

A

A

L

G

A

A

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R

M

L

A

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A

-

D

-

V

A

K

D

D

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S

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M

A

A

I

L

A

A

S

P

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L

A

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V

D

L

L

Y

Y

Q

G

L

L

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D

I

I

L

L

K

E

S

E

H

N

I

V

E

E

D

D

V

S

S

E

D

N

N

N

T

V

T

T

K

R

F

F

W

V

V

V

I

L

G

S

S

K

E

N

T

K

T

Q

C

W

-

A

-

R

P

P

S

E

G

N

E

D

D

E

K

R

-

I

-

V

T

T

A

T

L

F

V

V

L

F

S

R

I

V

Q

R

N
|
N

R
x
V

A

P

G

A

A

A

L
|
L

A

Y

K

K

I

A

L

L

D

G

S

G

F

F

A

A

K

T

R

N

D

G

I

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G
x
N

M
|
M

T
|
T

R
x
K

I

L

V

E

S
|
S

R

Y

P

Q

S

L

N

G

K

R

K

F

W

I

D

A

Y
x
T

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F
|
F

F

Y

I

A

D

D

I

I

V

E

G

G

H

H

Q

P

T

E

E

E

S

R

K

S

V

V

A

Q

E

L

A

A

L

L

N

E

E

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Q

F

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Y

Y

binding phenylalanine: N207 (= N292), V208 (≠ R293), L212 (= L297), N225 (≠ G310), M226 (= M311), T227 (= T312), K228 (≠ R313), S231 (= S316), T241 (≠ Y326), F243 (= F328)

7am0B Gqqa- a novel type of quorum quenching acylases (see paper)
28% identity, 73% coverage: 99:366/367 of query aligns to 4:274/278 of 7am0B

query
sites
7am0B

V

I

A

A

Y

F

L

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G

G

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E

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G

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A

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Y

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C

I

-

K

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F

A

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F

G

A

W

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T

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L

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C

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T

T

I

F

E

A

D

Q

V

T

F

I

K

A

S

A

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V

E

H

T

D

G

G

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R

T

A

H

E

Y

L

G

A

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M

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L

P

A

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C

E

E

N

N

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E

A

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N

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D

F

L

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A

R

I

V

H

E

H

H

C

C

L

L

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G

-

I

N

P

A

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K

S

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T

M

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G

A

S

D

I

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T

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K

R

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A

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H

H

A

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V

A

A

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M

G

A

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Q

C

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R

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G

W

I

L

I

K

T

N

E

-

L

N

G

L

L

-

D

P

P

G

V

V

V

E

E

L

F

E

D

A

T

V

A

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G

S

A

N

A

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M

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E

M

W

A

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Q

R

A

K

D

E

T

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S

-

L

-

A

V

A

A

I

V

A

A

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S

E

A

Q

L

A

A

V

E

L

L

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N

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G

L

L

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I

I

L

L

K

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S

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H

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E

E

D

D

V

A

S

T

D

H

N

N

T

T

K

R

F

F

W

Y

V

I

I

A

G

S

S

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E

R

-

P

-

A

T

T

T

L

C

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P

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S

P

G

G

E

P

D

G

-

F

K

M

T

T

A

T

L

L

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L

L

F

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R

I

V

Q
x
N

N
|
N

R
x
Q

A
x
P

G

G

A

A

L
|
L

A

Y

K

K

I

A

L

L

D

G

S

G

F

L

A

A

K

T

R

A

D

G

I

V

G
x
N

M
|
M

T
|
T

R
|
R

I

L

V

E

S

S

R

Y

P

M

S

L

N

E

N

G

K

S

K

F

W

S

D

A

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x
T

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F
|
F

F

L

I

M

D

D

I

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V

E

G

G

H

H

Q

P

T

E

E

A

S

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K

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V

L

A

A

E

R

A

A

L

L

N

D

E

E

V

L

E

-

A

-

Q

-

T

-

S

S

F

F

-

S

-

E

-

Q

K

E

L

I

L

L

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G

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Y

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P

V

A

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S

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L

F

binding phenylalanine: N199 (≠ Q291), N200 (= N292), Q201 (≠ R293), P202 (≠ A294), L205 (= L297), N218 (≠ G310), M219 (= M311), T220 (= T312), R221 (= R313), T234 (≠ Y326), F236 (= F328)

A0A0M0ELU2 Bifunctional N-acyl-homoserine lactone acylase/prephenate dehydratase; Bifunctional AHL acylase/PDT; Quorum quenching protein GqqA; EC 3.5.1.97; EC 4.2.1.51 from Komagataeibacter europaeus (Gluconacetobacter europaeus) (see paper)
28% identity, 73% coverage: 99:366/367 of query aligns to 7:277/281 of A0A0M0ELU2

query
sites
A0A0M0ELU2

V

I

A

A

Y

F

L

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G

G

P

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E

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A

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Y

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H

D

A

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A

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C

I

-

K

R

Q

Q

F

A

G
x
R

S

P

F

G

A

W

Q

T

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T

Y

L

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C

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Q

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T

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T

F

I

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A

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G

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A

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A

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N

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A

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A

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x
T

N

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P

G

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E

E

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-

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A

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D

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N

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F

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-

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x
P

P
|
P

S
x
P

G
|
G

E
x
P

D

G

-

F

K

M

T

T

A

T

L

L

V

L

L

F

S

R

I

V

Q

N

N

N

R

Q

A

P

G

G

A
|
A

L
|
L

A

Y

K

K

I

A

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L

D

G

S

G

F

L

A

A

K

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x
N

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|
M

T

T

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R

I

L

V

E

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S

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Y

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M

S

L

N

E

N

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F

F

F

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D

D

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G

G

H

H

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T

E

E

A

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K

P

V

L

A

A

E

R

A

A

L

L

N

D

E

E

V

L

E

-

A

-

Q

-

T

-

S

S

F
|
F

F

F

-

S

-

E

-

Q

K

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L

L

G

G

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P

V

A

S

S

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L

F

R25 (≠ G118) mutation to S: Affects dimerization. Loss of N-acyl-homoserine lactone acylase activity.
T118 (≠ K210) mutation to S: Does not affect N-acyl-homoserine lactone acylase activity.
PPPGP 187:191 (≠ CPSGE 277:281) mutation to AS: Loss of N-acyl-homoserine lactone acylase activity.
A207 (= A296) binding L-phenylalanine
L208 (= L297) binding L-phenylalanine
N221 (≠ G310) binding L-phenylalanine
M222 (= M311) binding L-phenylalanine
F261 (= F354) mutation to S: Loss of N-acyl-homoserine lactone acylase activity.

Sites not aligning to the query:

279:281 mutation Missing: Does not affect N-acyl-homoserine lactone acylase activity.

3luyA Putative chorismate mutase from bifidobacterium adolescentis
24% identity, 72% coverage: 98:362/367 of query aligns to 5:284/326 of 3luyA

query
sites
3luyA

K

K

V

L

A

F

Y

Y

L

L

G

G

P

P

E

Q

G

G

S

T

Y

F

T

T

H

H

A

Q

A

A

T

A

I

V

K

N

Q

A

F

A

G

Q

S

E

F

L

A

A

-

R

-

F

Q

E

P

P

-

Q

-

G

-

F

-

D

-

L

Y

M

P

P

V

M

S

D

T

D

I

V

E

P

D

Q

V

I

F

L

K

D

S

A

V

A

E

Q

T

H

G

G

V

-

T

D

H

G

Y

W

G

G

L

I

V

V

P

A

V

W

E

E

N

N

S

N

T

V

E

E

G

G

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Y

V

V

N

V

A

P

T

N

Q

L

D

D

S

A

L

L

I

I

T

D

M

A

-

K

-

D

-

L

-

V

-

G

-

F

-

A

R

R

A

V

H

G

V

V

T

N

G

V

E

E

V

F

D

D

L

-

A

-

I

-

H

-

C

A

L

Y

L

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S

A

N

Q

A

G

K

A

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D

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P

G

A

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I

A

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I

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A

V

T

A

A

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H

A

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Q

H

A

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L

L

G

A

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Q

C

C

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R

W

F

L

I

K

A

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E

N

H

L

R

P

-

G

-

V

L

E

S

L

T

E

Q

A

P

V

A

E

T

S

S

N

N

A

A

L

A

A

A

A

C

K

R

-

D

M

L

A

I

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P

A

G

D

E

T

I

S

A

L

F

A

G

-

P

A

A

I

I

A

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G

E

E

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-

A

-

V

-

L

L

Y

Y

Q

D

L

I

T

T

I

R

L

I

K

G

S

T

H

A

I

I

E

Q

D

D

V

Y

S

Q

D

G

N

A

T

A

T
|
T

K

D

F

F

W

L

V

V

I

L

G

S

S

P

E

R

T

A

T

E

C

V

-

A

-

R

-

L

-

A

-

K

P

P

S

R

G

A

E

E

-

A

-

N

-

V

-

E

-

Y

D

E

K

S

T

V

A

L

L

T

V

L

L

I

S

P

I

L

Q

V

N

T

R
x
G

A

P

G

G

A

V

L
|
L

A

A

K

N

I

L

L

L

D

D

S

V

F

F

A

R

K

D

R

A

D

G

I

L

G

N

M

M

T

T

R

S

I

F

V

I

S

S

R

R

P

P

S

I

N

K

N

G

K

R

K

T

W

G

D

T

Y
|
Y

V

S

F
|
F

F

I

I

V

D

T

I

L

V

D

G

A

H

A

Q

P

T

W

E

E

S

E

K

R

V

F

A

R

E

D

A

A

L

L

N

V

E

E

V

I

E

A

A

E

Q

H

T

G

S

D

F

W

F

A

K

K

L

T

L

L

G

A

S

V

Y

Y

P

P

active site: T177 (= T266)
binding 3-phenylpyruvic acid: G215 (≠ R293), L219 (= L297), Y248 (= Y326), F250 (= F328)

7alzA Gqqa- a novel type of quorum quenching acylases (see paper)
31% identity, 41% coverage: 218:367/367 of query aligns to 38:191/194 of 7alzA

query
sites
7alzA

L

I

E

A

A

A

V

V

E

H

S

D

N

G

A

R

L

A

A

E

A

L

K

A

M

M

A

L

Q

A

A

C

D

E

T

N

S

S

L

L

A

A

A

G

I

R

A

V

S

P

E

D

Q

I

A

H

A

A

V

L

L

L

Y

P

Q

E

-

A

-

G

L

L

T

F

I

I

L

V

K

G

S

E

H

H

I

F

E

Q

D

R

V

V

S

-

D

H

N

N

T

T

K

R

F

F

W

Y

V

I

I

A

G

S

S

R

E

R

-

P

-

A

T

T

T

L

C

P

P

P

S

P

G

G

E

P

D

G

-

F

K

M

T

T

A

T

L

L

V

L

L

F

S

R

I

V

Q

N

N

N

R
x
Q

A

P

G

G

A

A

L
|
L

A

Y

K

K

I

A

L

L

D

G

S

G

F

L

A

A

K

T

R

A

D

G

I

V

G
x
N

M
|
M

T
|
T

R
|
R

I

L

V

E

S

S

R

Y

P

M

S

L

N

E

N

G

K

S

K

F

W

S

D

A

Y
x
T

V

Q

F
|
F

F

L

I

M

D

D

I

V

V

E

G

G

H

H

Q

P

T

E

E

A

S

P

K

P

V

L

A

A

E

R

A

A

L

L

N

D

E

E

V

L

E

-

A

-

Q

-

T

-

S

S

F

F

-

S

-

E

-

Q

K

E

L

I

L

L

G

G

S

V

Y

Y

P

P

V

A

S

S

P

P

L

F

E

R

binding phenylalanine: Q117 (≠ R293), L121 (= L297), N134 (≠ G310), M135 (= M311), T136 (= T312), R137 (= R313), T150 (≠ Y326), F152 (= F328)

5j6fA Crystal structure of dah7ps-cm complex from geobacillus sp. With prephenate (see paper)
32% identity, 24% coverage: 9:95/367 of query aligns to 2:85/352 of 5j6fA

query
sites
5j6fA

E

E

Q

R

L

L

N

D

S

E

I

L

R

R

N

A

E

R

I

V

D

D

A

E

I

I

D

N

A

L

Q

Q

I

L

Q

L

E

K

L

L

I

I

G

N

E

E

R
|
R

A

G

R

R

C

L

A

V

Q

Q

K

E

V
x
I

A

G

D

K

I

I

K
|
K

T

E

Q

A

G

Q

G

G

K

T

V

H

E

R

A

-

V

-

F

-

Y
|
Y

R
x
D

P

P

E

V

R
|
R

E

E

A

R

Q

K

V
x
M

L

L

R

D

A

L

V

I

K

S

Q

E

R

H

N

N

N

D

S

G

P

P

L

F

S

E

D

T

N

S

D

T

M

L

A

Q

R

H

L

I

F

F

R

K

E

E

I

I

M

F

S

K

A

A

C

A

L

L

A

E

L

L

E

Q

Q

E

binding prephenic acid: R25 (= R32), I32 (≠ V39), K36 (= K43), Y44 (= Y54), D45 (≠ R55), R48 (= R58), M52 (≠ V62)

Sites not aligning to the query:

binding manganese (ii) ion: 124, 294, 320, 331

3tfcA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e in complex with phosphoenolpyruvate (see paper)
29% identity, 22% coverage: 14:95/367 of query aligns to 1:74/343 of 3tfcA

query
sites
3tfcA

I

L

R

R

N

T

E

Q

I

V

D

D

A

Q

I

L

D

N

A

I

Q

D

I

L

Q

L

E

E

L

L

I

I

G

S

E

K

R

R

A

A

R

N

C

L

A

V

Q

Q

K

E

V

I

A

G

D

K

I

I

K

K

T

L

Q

R

G

-

K

-

V

-

E

-

A

-

V

-

F

-

Y

F

R

D

P

P

E

L

R

R

E

E

A

R

Q

E

V

M

L

L

R

N

A

T

V

I

K

L

Q

A

R

A

N

N

N

E

S

G

P

P

L

F

S

E

D

D

N

S

D

T

M

V

A

Q

R

K

L

L

F

F

R

K

E

E

I

I

M

F

S

K

A

A

C

G

L

L

A

E

L

L

E

Q

Q

E

Sites not aligning to the query:

binding manganese (ii) ion: 113, 283, 309, 320
binding phosphoenolpyruvate: 137, 193, 196, 218, 248, 283

3nvtA 1.95 angstrom crystal structure of a bifunctional 3-deoxy-7- phosphoheptulonate synthase/chorismate mutase (aroa) from listeria monocytogenes egd-e (see paper)
29% identity, 22% coverage: 14:95/367 of query aligns to 2:75/345 of 3nvtA

query
sites
3nvtA

I

L

R

R

N

T

E

Q

I

V

D

D

A

Q

I

L

D

N

A

I

Q

D

I

L

Q

L

E

E

L

L

I

I

G

S

E

K

R

R

A

A

R

N

C

L

A

V

Q

Q

K

E

V

I

A

G

D

K

I

I

K

K

T

G

Q

R

G

-

K

-

V

-

E

-

A

-

V

-

F

-

Y

F

R

D

P

P

E

L

R

R

E

E

A

R

Q

E

V

M

L

L

R

N

A

T

V

I

K

L

Q

A

R

A

N

N

N

E

S

G

P

P

L

F

S

E

D

D

N

S

D

T

M

V

A

Q

R

K

L

L

F

F

R

K

E

E

I

I

M

F

S

K

A

A

C

G

L

L

A

E

L

L

E

Q

Q

E

Sites not aligning to the query:

binding manganese (ii) ion: 114, 284, 310, 321

P00439 Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenase; EC 1.14.16.1 from Homo sapiens (Human) (see 48 papers)
44% identity, 13% coverage: 285:332/367 of query aligns to 36:83/452 of P00439

query
sites
P00439

A

S

L

L

V

I

L
x
F

S
|
S

I
x
L

Q
x
K

N

E

R

E

A
x
V

G
|
G

A
|
A

L
|
L

A

A

K

K

I

V

L

L

D
x
R

S

L

F
|
F

A
x
E

K

E

R

N

D

D

I

V

G
x
N

M
x
L

T
|
T

R
x
H

I
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I

V

E

S
|
S

R
|
R

P

P

S

S

N

R

N

L

K

K

W

D

D
x
E

Y

Y

V

E

F

F

I

T

D

H

I

L

F39 (≠ L288) to L: in PAH deficiency; haplotype 1; dbSNP:rs62642926; natural variant: Missing (in PAH deficiency; severe; haplotypes 9,21)
S40 (= S289) to L: in PAH deficiency; severe; dbSNP:rs62642938
L41 (≠ I290) to F: in PAH deficiency; severe; dbSNP:rs62642928; to P: in PAH deficiency; mild; dbSNP:rs62642916
K42 (≠ Q291) to I: in PAH deficiency; severe; haplotype 21; dbSNP:rs62635346
V45 (≠ A294) to A: in PAH deficiency; severe; dbSNP:rs1592988883
G46 (= G295) to S: in PAH deficiency; severe; haplotype 5; significantly reduces phenylalanine binding; dbSNP:rs74603784
A47 (= A296) to V: in PAH deficiency; haplotype 4; significantly reduces phenylalanine binding; dbSNP:rs118203925
L48 (= L297) to S: in PAH deficiency; mild; haplotypes 3,4; dbSNP:rs5030841
R53 (≠ D302) to H: in PAH deficiency; severe; dbSNP:rs118092776
F55 (= F304) to L: in PAH deficiency; does not affect oligomerization; results in loss of substrate activation; dbSNP:rs199475598
E56 (≠ A305) to D: in PAH deficiency; severe; haplotype 10; dbSNP:rs199475567
N61 (≠ G310) to D: in PAH deficiency; severe; dbSNP:rs199475651
L62 (≠ M311) to P: in PAH deficiency; severe; dbSNP:rs1877437661
TH 63:64 (≠ TR 312:313) natural variant: TH -> PN (in PAH deficiency; severe; haplotype 1; abolishes phenylalanine binding)
I65 (= I314) to N: in PAH deficiency; severe; dbSNP:rs75193786; to S: in PAH deficiency; severe; results in disturbed oligomerization; results in loss of substrate activation; dbSNP:rs75193786; to T: in PAH deficiency; severe; haplotypes 1,5,9,21,B; abolishes phenylalanine binding; dbSNP:rs75193786; to V: in PAH deficiency; dbSNP:rs199475643
S67 (= S316) to P: in PAH deficiency; severe; haplotype 4; dbSNP:rs5030842
R68 (= R317) to S: in PAH deficiency; severe; haplotype 1; dbSNP:rs76394784
E76 (≠ D325) to A: in PAH deficiency; severe; dbSNP:rs62507347; to G: in PAH deficiency; dbSNP:rs62507347

Sites not aligning to the query:

16 modified: Phosphoserine; by PKA; S → P: in PAH deficiency; severe; uncertain significance; dbSNP:rs62642946
20 Q → L: in PAH deficiency; mild; dbSNP:rs199475662
84 D → Y: in PAH deficiency; severe; haplotype 4; dbSNP:rs62514902
87 S → R: in PAH deficiency; haplotype 1; dbSNP:rs62516151
92 T → I: in PAH deficiency; severe; dbSNP:rs62514903
94 natural variant: Missing (in PAH deficiency; mild; haplotype 2)
98 L → S: in PAH deficiency; dbSNP:rs62517167
104 A → D: in PAH deficiency; mild; haplotype 1; dbSNP:rs62642929
110 S → C: in PAH deficiency; mild
121 F → L: in PAH deficiency; mild
124 T → I: in PAH deficiency; severe; haplotype 28; dbSNP:rs199475571
129 D → Y: in PAH deficiency; severe; dbSNP:rs199475606
143 D → G: in PAH deficiency; severe; haplotype 11; dbSNP:rs199475572
145 D → V: in PAH deficiency; severe; dbSNP:rs140175796
146 H → Y: in PAH deficiency; severe; dbSNP:rs199475599
148 G → S: in PAH deficiency; severe; haplotypes 1,2,7; dbSNP:rs80297647
151 D → H: in PAH deficiency; severe; haplotypes 1,8; dbSNP:rs199475597
154 Y → N: in PAH deficiency; severe; dbSNP:rs199475587
155 R → P: in PAH deficiency; severe; dbSNP:rs199475663
157 R → N: in PAH deficiency; severe; 5% activity; requires 2 nucleotide substitutions; dbSNP:rs1565853495; R → S: in PAH deficiency; severe; dbSNP:rs199475612
158 R → Q: in PAH deficiency; severe; haplotypes 1,2,4,7,16, 28; dbSNP:rs5030843; R → W: in PAH deficiency; severe; dbSNP:rs75166491
160 Q → P: in PAH deficiency; severe; dbSNP:rs199475601
161 F → S: in PAH deficiency; severe; haplotype 4; dbSNP:rs79635844
164 I → T: in PAH deficiency; severe; haplotype 1; dbSNP:rs199475595
167 N → I: in PAH deficiency; severe; dbSNP:rs77554925; N → S: in PAH deficiency; mild; dbSNP:rs77554925
169 R → H: in PAH deficiency; severe; dbSNP:rs199475679
170 H → D: in PAH deficiency; mild; dbSNP:rs199475655; H → Q: in PAH deficiency; severe; does not affect oligomerization; dbSNP:rs199475652; H → R: in PAH deficiency; severe; dbSNP:rs199475573
171 G → A: in PAH deficiency; severe; haplotype 1; dbSNP:rs199475596; G → R: in PAH deficiency; severe; dbSNP:rs199475613
173 P → T: in PAH deficiency; severe; haplotype 4; dbSNP:rs199475574
174 I → T: in PAH deficiency; severe; haplotype 1; dbSNP:rs138809906; I → V: in PAH deficiency; severe; dbSNP:rs199475632
175 P → A: in PAH deficiency; severe; dbSNP:rs199475604
176 R → L: in PAH deficiency; dbSNP:rs74486803; R → P: in PAH deficiency; severe; dbSNP:rs74486803
177 V → L: in PAH deficiency; severe; haplotype 6; dbSNP:rs199475602; V → M: in PAH deficiency; mild; dbSNP:rs199475602
178 E → G: in PAH deficiency; dbSNP:rs77958223
183 E → Q: in PAH deficiency; severe; dbSNP:rs199475664
190 V → A: in PAH deficiency; severe; haplotype 3; dbSNP:rs62514919
194 L → P: in PAH deficiency; severe; dbSNP:rs5030844
196 S → Y: in PAH deficiency; mild; dbSNP:rs865899394
201 H → R: in PAH deficiency; severe; dbSNP:rs62517180; H → Y: in PAH deficiency; haplotype 1; dbSNP:rs62517205
204 Y → C: in PAH deficiency; mild; haplotypes 3,4; dbSNP:rs62514927
205 E → A: in PAH deficiency; severe; dbSNP:rs62508593
206 Y → D: in PAH deficiency; severe; dbSNP:rs62517170
207 N → D: in PAH deficiency; severe; dbSNP:rs62508572; N → S: in PAH deficiency; severe; haplotype 4; dbSNP:rs62508721
211 P → T: in PAH deficiency; severe; haplotype 4; dbSNP:rs62514931
212 L → P: in PAH deficiency; severe; dbSNP:rs62517198
213 L → P: in PAH deficiency; severe; dbSNP:rs62516109
217 C → G: in PAH deficiency; severe; dbSNP:rs62508718
218 G → V: in PAH deficiency; severe; haplotypes 1,2; dbSNP:rs62514933
221 E → G: in PAH deficiency; severe; haplotype 4; dbSNP:rs62514934
222 D → V: in PAH deficiency; severe; haplotypes 3,4; dbSNP:rs62507319
224 I → M: in PAH deficiency; severe; haplotype 4; dbSNP:rs199475576
225 P → R: in PAH deficiency; severe; dbSNP:rs62517204; P → T: in PAH deficiency; severe; haplotype 1; dbSNP:rs199475589
226 Q → H: in PAH deficiency; severe; dbSNP:rs62508615
230 V → I: in PAH deficiency; haplotype 4; dbSNP:rs62516152
231 S → F: in PAH deficiency; severe; dbSNP:rs62508577; S → P: in PAH deficiency; severe; dbSNP:rs5030845
233 F → L: in PAH deficiency; severe; haplotypes 2,3; dbSNP:rs62517208
238 T → P: in PAH deficiency; severe; haplotype 4; dbSNP:rs199475577
239 G → S: in PAH deficiency; severe; dbSNP:rs62517178
240 F → S: in PAH deficiency; severe; dbSNP:rs62508594
241 R → C: in PAH deficiency; haplotype 34; dbSNP:rs76687508; R → H: in PAH deficiency; severe; haplotypes 1,5; dbSNP:rs62508730; R → L: in PAH deficiency; severe; dbSNP:rs62508730
242 L → F: in PAH deficiency; severe; dbSNP:rs199475578
243 R → Q: in PAH deficiency; haplotypes 4,7,9; dbSNP:rs62508588
244 P → L: in PAH deficiency; severe; haplotype 12; dbSNP:rs118203923
245 V → A: in PAH deficiency; haplotypes 3,7; dbSNP:rs76212747; V → E: in PAH deficiency; severe; haplotype 11; dbSNP:rs76212747; V → L: in PAH deficiency; severe; dbSNP:rs62508694
246 A → D: in PAH deficiency; severe; dbSNP:rs199475610
247 G → V: in PAH deficiency; severe; haplotype 4; dbSNP:rs199475579
248 L → P: in PAH deficiency; severe; dbSNP:rs62507340
249 L → F: in PAH deficiency; severe; haplotype 1; dbSNP:rs74503222
252 R → G: in PAH deficiency; severe; haplotype 7; dbSNP:rs5030847; R → Q: in PAH deficiency; severe; haplotype 1; dbSNP:rs62644503; R → W: in PAH deficiency; severe; haplotypes 1,6,7,8,42, 69; complete loss of activity; dbSNP:rs5030847
255 L → S: in PAH deficiency; severe; haplotype 36; dbSNP:rs62642930; L → V: in PAH deficiency; severe; haplotypes 18,21; dbSNP:rs62642931
257 G → C: in PAH deficiency; severe; dbSNP:rs5030848
259 A → T: in PAH deficiency; severe; haplotype 3; dbSNP:rs62642932; A → V: in PAH deficiency; severe; haplotypes 7,42; dbSNP:rs118203921
261 R → P: in PAH deficiency; severe; dbSNP:rs5030849; R → Q: in PAH deficiency; haplotypes 1,2,4,22, 24,28; dbSNP:rs5030849
263 F → L: in PAH deficiency; severe; dbSNP:rs62642944
264 H → L: in PAH deficiency; severe; dbSNP:rs199475580
265 C → G: in PAH deficiency; severe; dbSNP:rs62517181
269 I → L: in PAH deficiency; dbSNP:rs62508692
270 R → K: in PAH deficiency; severe; dbSNP:rs62514950; R → S: in PAH deficiency; severe; haplotype 1; dbSNP:rs62514951
271 H → Y: in PAH deficiency; severe; dbSNP:rs62517164
273 S → F: in PAH deficiency; severe; haplotype 7; dbSNP:rs62514953
274 K → E: in PAH deficiency; severe; dbSNP:rs142934616
275 P → L: in PAH deficiency; severe; reduced activity; increased affinity for the substrate; mildly reduced substrate activation; decreased cofactor affinity; dbSNP:rs62508715
276 M → I: in PAH deficiency; severe; dbSNP:rs62514954; M → V: in PAH deficiency; severe; haplotype 4; dbSNP:rs62516149
277 Y → C: in PAH deficiency; severe; dbSNP:rs62516155; Y → D: in PAH deficiency; severe; haplotype 2; dbSNP:rs78655458
278 T → A: in PAH deficiency; severe; dbSNP:rs62516156; T → N: in PAH deficiency; severe; dbSNP:rs62507262
280 E → K: in PAH deficiency; severe; haplotypes 1,2,4,16,38; partial residual activity; dbSNP:rs62508698
281 P → L: in PAH deficiency; severe; haplotypes 1,4; dbSNP:rs5030851
282 D → N: in PAH deficiency; severe; haplotype 1; dbSNP:rs199475582
283 I → F: in PAH deficiency; severe; haplotype 21; dbSNP:rs62517168; I → N: in PAH deficiency; severe; dbSNP:rs62508693; I→C: Loss of positive cooperativity and reduction of fold-activation by L-Phe preincubation.
290 H → Y: in PAH deficiency; severe; dbSNP:rs1486763160
297 R → C: in PAH deficiency; severe; haplotype 4; dbSNP:rs62642945; R → H: in PAH deficiency; severe; dbSNP:rs62642939
299 F → C: in PAH deficiency; severe; haplotype 8; dbSNP:rs62642933
300 A → S: in PAH deficiency; haplotype 1; does not affect oligomerization; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs5030853; A → V: in PAH deficiency; severe; dbSNP:rs199475609
303 S → P: in PAH deficiency; severe; haplotype 5; dbSNP:rs199475608
304 Q → R: in PAH deficiency; severe; dbSNP:rs199475592
306 I → V: in PAH deficiency; haplotype 4; dbSNP:rs62642934
309 A → D: in PAH deficiency; severe; haplotype 7; dbSNP:rs62642935; A → V: in PAH deficiency; severe; dbSNP:rs62642935
310 S → F: in PAH deficiency; severe; haplotype 7; dbSNP:rs62642913; S → Y: in PAH deficiency; mild; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs62642913
311 L → P: in PAH deficiency; severe; haplotypes 1,7,10; dbSNP:rs62642936
314 P → H: in PAH deficiency; severe; dbSNP:rs62642940; P → S: in PAH deficiency; mild; does not affect oligomerization; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs199475650
318 I → T: in PAH deficiency; severe; partial loss of activity; dbSNP:rs62642918
322 A → G: in PAH deficiency; severe; haplotype 12; dbSNP:rs62514958; A → T: in PAH deficiency; severe; haplotype 1; dbSNP:rs62514957; A → V: in PAH deficiency; severe; dbSNP:rs62514958
325 Y → C: in PAH deficiency; severe; dbSNP:rs62508578
330 E → D: in PAH deficiency; severe; dbSNP:rs62508580
331 F → L: in PAH deficiency; severe; haplotype 1; dbSNP:rs62517179
333 L → F: in PAH deficiency; severe; dbSNP:rs62516060
334 C → S: in PAH deficiency; severe; dbSNP:rs62517174
337 G → V: in PAH deficiency; severe; dbSNP:rs62517206
338 D → Y: in PAH deficiency; severe; haplotype 4; dbSNP:rs62516150
341 K → R: in PAH deficiency; severe; dbSNP:rs62516153; K → T: in PAH deficiency; severe; dbSNP:rs62516153
342 A → T: in PAH deficiency; severe; haplotype 5; dbSNP:rs62507282
343 Y → C: in PAH deficiency; severe; dbSNP:rs62507265
344 G → R: in PAH deficiency; severe; dbSNP:rs62508679; G → V: in PAH deficiency; severe; dbSNP:rs62508582
345 A → S: in PAH deficiency; severe; dbSNP:rs62516062; A → T: in PAH deficiency; severe; haplotype 7; dbSNP:rs62516062
347 L → F: in PAH deficiency; severe; dbSNP:rs62516154
348 L → V: in PAH deficiency; mild; haplotype 9; dbSNP:rs62516092
349 S → L: in PAH deficiency; severe; dbSNP:rs62507279; S → P: in PAH deficiency; severe; haplotypes 1,4; dbSNP:rs62508646
350 S → T: in PAH deficiency; severe; haplotype 2; dbSNP:rs62517183
357 C → G: in PAH deficiency; severe; dbSNP:rs62508595
362 P → T: in PAH deficiency; severe; dbSNP:rs62507329
364 natural variant: Missing (in PAH deficiency; severe; haplotype 5; dbSNP:rs62516096)
364:368 natural variant: Missing (in PAH deficiency; severe; dbSNP:rs62516096)
366 P → H: in PAH deficiency; severe; dbSNP:rs62516098
372 T → S: in PAH deficiency; severe; dbSNP:rs62517163
377 Y → C: in PAH deficiency; severe; haplotype 4; dbSNP:rs62642942
380 T → M: in PAH deficiency; haplotype 4; dbSNP:rs62642937
386 Y → C: in PAH deficiency; severe; dbSNP:rs62516141
387 Y → H: in PAH deficiency; severe; haplotype 1; dbSNP:rs62517194
388 V → L: in PAH deficiency; severe; dbSNP:rs62516101; V → M: in PAH deficiency; severe; haplotypes 1,4; dbSNP:rs62516101
390 E → G: in PAH deficiency; haplotype 4; dbSNP:rs5030856
394 D → A: in PAH deficiency; severe; dbSNP:rs62516102; D → H: in PAH deficiency; severe; dbSNP:rs62516142
395 A → G: in PAH deficiency; severe; dbSNP:rs62508736; A → P: in PAH deficiency; severe; haplotype 1; dbSNP:rs62516103
403 A → V: in PAH deficiency; haplotype 43; dbSNP:rs5030857
407 P → L: in PAH deficiency; severe; dbSNP:rs62644473; P → S: in PAH deficiency; severe; dbSNP:rs62644465
408 R → Q: in PAH deficiency; severe; haplotypes 4,12; dbSNP:rs5030859; R → W: in PAH deficiency; haplotypes 1,2,4,5,13,34,41,44; most common mutation; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs5030858
410 F → S: in PAH deficiency; mild; dbSNP:rs62644475
413 R → P: in PAH deficiency; haplotype 4; dbSNP:rs79931499; R → S: in PAH deficiency; severe; haplotype 1; dbSNP:rs62644467
414 Y → C: in PAH deficiency; haplotype 4; does not affect oligomerization; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs5030860
415 D → N: in PAH deficiency; haplotype 1; dbSNP:rs62644499
417 Y → H: in PAH deficiency; severe; reduction in activity is probably due to a global conformational change in the protein that reduces allostery; dbSNP:rs62644471
418 T → P: in PAH deficiency; severe; haplotype 4; dbSNP:rs62644501
421 I → S: in PAH deficiency; severe; dbSNP:rs199475696
430 L → P: in PAH deficiency; severe; dbSNP:rs199475607
447 A → D: in PAH deficiency; severe; dbSNP:rs76542238

5fgjA Structure of tetrameric rat phenylalanine hydroxylase, residues 1-453 (see paper)
47% identity, 12% coverage: 285:329/367 of query aligns to 17:61/428 of 5fgjA

query
sites
5fgjA

A

S

L

L

V

I

L

F

S

S

I

L

Q

K

N

E

R

E

A

V

G

G

A

A

L

L

A

A

K

K

I

V

L

L

D

R

S

L

F

F

A

E

K

E

R

N

D

D

I

I

G

N

M

L

T

T

R

H

I

I

V

E

S

S

R

R

P

P

S

S

N

R

N

L

K

N

K

K

W

D

D

E

Y

Y

V

E

F

F

Sites not aligning to the query:

active site: 263, 268, 308, 327
binding fe (iii) ion: 263, 268, 308

P04176 Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenase; EC 1.14.16.1 from Rattus norvegicus (Rat) (see 2 papers)
47% identity, 12% coverage: 285:329/367 of query aligns to 36:80/453 of P04176

query
sites
P04176

A

S

L

L

V

I

L

F

S

S

I

L

Q

K

N

E

R

E

A

V

G

G

A

A

L

L

A

A

K

K

I

V

L

L

D

R

S

L

F

F

A

E

K

E

R

N

D

D

I

I

G

N

M

L

T

T

R

H

I

I

V

E

S

S

R

R

P

P

S

S

N

R

N

L

K

N

K

K

W

D

D

E

Y

Y

V

E

F

F

Sites not aligning to the query:

16 modified: Phosphoserine; by PKA
285 binding Fe cation
290 binding Fe cation
330 binding Fe cation

5fiiB Structure of a human aspartate kinase, chorismate mutase and tyra domain. (see paper)
47% identity, 12% coverage: 285:329/367 of query aligns to 3:47/78 of 5fiiB

query
sites
5fiiB

A

S

L

L

V

I

L

F

S

S

I

L

Q

K

N
x
E

R
x
E

A

V

G

G

A

A

L
|
L

A

A

K

K

I

V

L

L

D

R

S

L

F

F

A

E

K

E

R

N

D

D

I

V

G
x
N

M
x
L

T

T

R
x
H

I
|
I

V

E

S
|
S

R

R

P

P

S

S

N

R

N

L

K

K

W

D

D

E

Y
|
Y

V

E

F
|
F

F

F

binding phenylalanine: E10 (≠ N292), E11 (≠ R293), L15 (= L297), N28 (≠ G310), L29 (≠ M311), H31 (≠ R313), I32 (= I314), S34 (= S316), Y44 (= Y326), F46 (= F328)

6al9B Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
32% identity, 21% coverage: 11:86/367 of query aligns to 5:75/91 of 6al9B

query
sites
6al9B

L

L

N

E

S

N

I

L

R

R

N

A

E

E

I

I

D

D

A

A

I

L

D

D

A

N

Q

E

I

L

Q

S

E

D

L

L

I

L

G

D

E

K

R

R

A

L

R

E

C

I

A

A

Q

L

K

K

V
x
I

A

A

D

L

I

I

K

K

T

Q

Q

-

G

-

K

-

V

-

E

E

A

S

V

P

F

I

Y

Y

R

C

P

P

E

K

R

R

E

E

A

Q

Q

E

V

I

L

L

R

K

A

R

V

L

K

S

Q

Q

R

R

N

D

N

F

S

K

P

H

L

L

S

N

D

G

N

E

D

I

M

L

A

T

R

G

L

F

F

Y

R

T

E

E

I

V

binding pyruvic acid: I33 (≠ V39)

Sites not aligning to the query:

binding pyruvic acid: 83

6al9A Crystal structure of chorismate mutase from helicobacter pylori in complex with prephenate
32% identity, 21% coverage: 11:86/367 of query aligns to 4:74/90 of 6al9A

query
sites
6al9A

L

L

N

E

S

N

I

L

R

R

N

A

E

E

I

I

D

D

A

A

I

L

D

D

A

N

Q

E

I

L

Q

S

E

D

L

L

I

L

G

D

E

K

R
|
R

A

L

R

E

C

I

A

A

Q

L

K

K

V

I

A

A

D

L

I

I

K

K

T

Q

Q

-

G

-

K

-

V

-

E

E

A

S

V

P

F

I

Y

Y

R
x
C

P

P

E

K

R
|
R

E
|
E

A

Q

Q

E

V

I

L

L

R

K

A

R

V

L

K

S

Q

Q

R

R

N

D

N

F

S

K

P

H

L

L

S

N

D

G

N

E

D

I

M

L

A

T

R

G

L

F

F

Y

R

T

E

E

I

V

binding prephenic acid: R25 (= R32), C43 (≠ R55), R46 (= R58), E47 (= E59)

Sites not aligning to the query:

binding prephenic acid: 75, 78, 82

P16331 Phenylalanine-4-hydroxylase; PAH; Phe-4-monooxygenase; EC 1.14.16.1 from Mus musculus (Mouse) (see paper)
44% identity, 12% coverage: 285:329/367 of query aligns to 36:80/453 of P16331

query
sites
P16331

A

S

L

L

V

I

L

F

S

S

I

L

Q

K

N

E

R

E

A

V

G

G

A

A

L

L

A

A

K

K

I

V

L

L

D

R

S

L

F

F

A

E

K

E

R

N

D

E

I

I

G

N

M

L

T

T

R

H

I

I

V

E

S

S

R

R

P

P

S

S

N

R

N

L

K

N

K

K

W

D

D

E

Y

Y

V

E

F

F

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
106 V→A: Mutant mice have mild features of phenylketonuria.
263 F→S: Mutant mice have features of phenylketonuria.

Query Sequence

>WP_029934730.1 NCBI__GCF_000711195.1:WP_029934730.1
MTPNTQEAEQLNSIRNEIDAIDAQIQELIGERARCAQKVADIKTQGGKVEAVFYRPEREA
QVLRAVKQRNNSPLSDNDMARLFREIMSACLALEQPIKVAYLGPEGSYTHAATIKQFGSF
AQPYPVSTIEDVFKSVETGVTHYGLVPVENSTEGVVNATQDSLITMRAHVTGEVDLAIHH
CLLSNAKDMGSITKVVAHAQALGQCRSWLKNNLPGVELEAVESNALAAKMAQADTSLAAI
ASEQAAVLYQLTILKSHIEDVSDNTTKFWVIGSETTCPSGEDKTALVLSIQNRAGALAKI
LDSFAKRDIGMTRIVSRPSNNKKWDYVFFIDIVGHQTESKVAEALNEVEAQTSFFKLLGS
YPVSPLE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory