SitesBLAST

A9 (≠ C6) binding AMP
NPFC 39:42 (≠ NPYD 34:37) binding AMP
C66 (≠ I60) binding AMP
123:134 (vs. 118:129, 42% identical) binding AMP
D128 (= D123) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
T154 (≠ D149) to M: in dbSNP:rs1130426
R164 (≠ K159) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
E165 (≠ D160) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
183:205 (vs. 178:200, 65% identical) Recognition loop
L195 (= L190) mutation to A: Severely impaired in complex formation with ACADM.
K200 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
KAK 200:202 (= KAK 195:197) mutation to RAR: Does not abolish methylation by ETFBKMT.
KAKK 200:203 (= KAKK 195:198) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
K203 (= K198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.

Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
37% identity, 90% coverage: 1:234/259 of query aligns to 4:236/255 of Q9DCW4

query
sites
Q9DCW4

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K200 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
K203 (= K198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
37% identity, 90% coverage: 1:234/259 of query aligns to 1:233/252 of 2a1uB

query
sites
2a1uB

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binding adenosine monophosphate: A6 (≠ C6), V7 (≠ L7), K8 (= K8), C39 (≠ D37), C63 (≠ I60), V101 (≠ I97), L119 (≠ C117), G120 (= G118), Q122 (≠ M120), A123 (= A121), D126 (= D124), C128 (≠ S126), N129 (≠ A127), Q130 (= Q128), T131 (≠ V129)
binding flavin-adenine dinucleotide: P37 (= P35), L182 (≠ Q180)

1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
37% identity, 90% coverage: 1:234/259 of query aligns to 1:233/246 of 1efpB

query
sites
1efpB

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binding adenosine monophosphate: P6 (≠ C6), V7 (≠ L7), D39 (= D37), I63 (= I60), V101 (≠ I97), A119 (≠ C117), G120 (= G118), Q122 (≠ M120), A123 (= A121), N126 (≠ D124), M128 (≠ S126), N129 (≠ A127), T131 (≠ V129)
binding flavin-adenine dinucleotide: Y13 (≠ F11), L182 (≠ Q180)

Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
36% identity, 90% coverage: 1:234/259 of query aligns to 4:236/255 of Q2TBV3

query
sites
Q2TBV3

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K200 (= K195) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
K203 (= K198) modified: N6,N6,N6-trimethyllysine; by ETFBKMT

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
36% identity, 98% coverage: 1:253/259 of query aligns to 1:257/262 of 6fahB

query
sites
6fahB

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D

G

L

L

D

S

E

P

E

E

E

A

I

C

-

G

-

L

-

N

A

A

A

S

K

P

T
|
T

E

Q

T

V

I

F

D

R

V

S

F

F

L

S

P

P

K

A

K

K

Q

G

A

G

G

G

K

E

I

M

L

I

Q

T

G

G

-

T

D

T

I

V

K

N

D

E

Q

V

A

A

K

G

E

S

L

L

V

V

Q

S

L

K

L

L

R

K

binding flavin-adenine dinucleotide: C6 (= C6), A7 (≠ L7), K8 (= K8), D38 (= D37), T61 (= T59), M62 (≠ I60), A93 (≠ E91), D94 (= D93), T95 (≠ E94), T98 (≠ I97), L102 (= L101), G118 (= G118), Q120 (≠ M120), G124 (≠ D124), T126 (≠ S126), A127 (= A127), V129 (= V129), T223 (= T220)

5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
35% identity, 98% coverage: 1:253/259 of query aligns to 1:255/260 of 5ol2B

query
sites
5ol2B

M

M

N

N

I

I

L

V

V

V

C
|
C

L
x
I

K
|
K

Q

Q

T

V

F

P

D

D

T

T

E

T

E

E

-

V

R

K

I

L

T

D

I

P

E

N

N

T

G

G

R

T

I

L

S

I

E

R

D

D

G

G

V

V

E

P

F

S

V

I

I

I

N

N

P

P

Y

D

D
|
D

E

K

Y

A

A

G

V

L

E

E

A

A

I

I

R

K

L

L

K

K

E

E

K

E

H

M

G

G

G

A

E

H

V

V

T

T

V

V

I

I

T

T

I
x
M

G

G

P

P

E

P

R

Q

A

A

E

D

Q

M

A

A

L

L

R

K

T

E

A

A

M

L

A

A

M

M

G

G

A

A

D

D

K

R

G

G

V

I

I

L

V

L

D

-

I

T

E

D

E

R

L

A

E

F

E

A

E

G

P
x
A

D
|
D

E
x
T

H

W

T

A

I
x
T

A

S

K

S

I

A

L

L

A

A

A

G

T

A

I

L

K

K

E

N

E

I

D

D

L

-

A

-

F

F

D

D

I

I

L

I

C
x
A

G
|
G

Y

R

M

Q

A
|
A

V

I

D

D

D
x
G

G

D

S
x
T

A
|
A

Q

Q

V
|
V

G

G

P

P

R

Q

L

I

A

A

E

E

L

H

L

L

E

N

I

L

P

P

H

S

I

I

S

T

T

Y

I

A

T

E

K

E

L

I

D

K

I

T

D

E

G

G

N

E

K

Y

V

V

S

L

V

V

E

K

K

R

D

Q

V

F

E

E

G

D

D

C

V

C

E

H

L

D

I

L

D

K

S

V

S

K

L

M

P

P

I

C

L

L

V

I

T

T

A

T

Q

L

Q

K

G

D

L

M

N

N

D

T

P

P

R

R

Y

Y

P

M

S

K

L

V

P

G

G

R

I

I

M

Y

K

D

A

A

K

F

K

E

K

N

P

D

L

V

T

V

R

E

-

T

L

W

E

T

L

V

D

K

D

D

L

I

D

E

L

V

D

D

E

P

E

S

E

N

I

L

A

G

A

L

K

K

-

G

-

S

-

P

T

T

E

S

T

V

I

F

D

K

V

S

F

F

L

T

P

K

P

S

K

V

K

K

Q

P

A

A

G

G

K

T

I

I

L

Y

Q

N

G

E

D

D

I

A

K

K

D

T

Q

S

A

A

K

G

E

I

L

I

V

I

Q

D

L

K

L

L

R

K

binding flavin-adenine dinucleotide: C6 (= C6), I7 (≠ L7), K8 (= K8), D38 (= D37), M61 (≠ I60), A92 (≠ P92), D93 (= D93), T94 (≠ E94), T97 (≠ I97), A115 (≠ C117), G116 (= G118), A119 (= A121), G122 (≠ D124), T124 (≠ S126), A125 (= A127), V127 (= V129)

4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
39% identity, 76% coverage: 1:198/259 of query aligns to 1:196/263 of 4l2iB

query
sites
4l2iB

M

M

N

N

I

I

L

V

V

V

C
|
C

L
x
V

K
|
K

Q

Q

T

V

F

P

D

D

T

T

E

A

E

E

-

M

R

K

I

I

T

D

I

P

E

V

N

T

G

N

R

N

I

L

S

V

E

R

D

D

G

G

V

V

E

T

F

N

V

I

I

M

N

N

P

P

Y
|
Y

D
|
D

E

Q

Y
|
Y

A

A

V

L

E

E

E

T

A

A

I

L

R

Q

L

L

K

K

E

D

K

E

H

L

G

G

G

A

E

H

V

V

T

T

V

V

I

I

T
|
T

I
x
M

G
|
G

P
|
P

E

P

R

H

A

A

E

E

Q

S

A

V

L

L

R

R

T

D

A

C

M

L

A

A

M

V

G

G

A

A

D

D

K

E

G

A

V

K

I

L

V

V

D
x
S

I

-

E
x
D

E

R

L

A

E

F

E

G

E
x
G

P
x
A

D
|
D

E
x
T

H

L

T

A

I
x
T

A

S

K

A

I

A

L
x
M

A

A

A

N

T

T

I

I

K

K

E

H

E

F

D

G

L

V

A

P

F

-

D

D

I

L

I

I

L

L

C
|
C

G
|
G

Y

R

M
x
Q

A
|
A

V

I

D

D

D
x
G

G

D

S
x
T

A
|
A

Q

Q

V
|
V

G

G

P

P

R

E

L

I

A

A

E

E

L

H

L

L

E

G

I

L

P

P

H

Q

I

V

S

T

T

A

I

A

T

L

K

K

L

V

D

Q

I

V

D

K

G

D

N

D

K

T

V

V

S

V

V

V

E

D

K

R

D

D

V

N

E

E

G

Q

D

M

V

S

E

M

L

T

I

F

D

T

S

M

S

K

L

M

P

P

I

C

L

V

V

V

T

T

A

V

Q

M

Q

R

G

S

L

-

N

K

D

D

P

L

R

R

Y

F

P

A

S

S

L

I

P

R

G

G

I

K

M

M

K

K

A

A

K

R

K

K

binding flavin-adenine dinucleotide: C6 (= C6), V7 (≠ L7), K8 (= K8), Y37 (= Y36), D38 (= D37), Y40 (= Y39), T60 (= T59), M61 (≠ I60), A92 (≠ P92), D93 (= D93), T94 (≠ E94), T97 (≠ I97), M101 (≠ L101), C116 (= C117), G117 (= G118), Q119 (≠ M120), A120 (= A121), G123 (≠ D124), T125 (≠ S126), A126 (= A127), V128 (= V129)
binding nicotinamide-adenine-dinucleotide: M61 (≠ I60), G62 (= G61), P63 (= P62), S85 (≠ D84), D86 (≠ E86), G91 (≠ E91)

4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
39% identity, 76% coverage: 1:198/259 of query aligns to 1:196/263 of 4kpuB

query
sites
4kpuB

M

M

N

N

I

I

L

V

V

V

C
|
C

L
x
V

K
|
K

Q

Q

T

V

F

P

D

D

T

T

E

A

E

E

-

M

R

K

I

I

T

D

I

P

E

V

N

T

G

N

R

N

I

L

S

V

E

R

D

D

G

G

V

V

E

T

F

N

V

I

I

M

N

N

P

P

Y
|
Y

D
|
D

E

Q

Y
|
Y

A

A

V

L

E

E

E

T

A

A

I

L

R

Q

L

L

K

K

E

D

K

E

H

L

G

G

G

A

E

H

V

V

T

T

V

V

I

I

T

T

I
x
M

G

G

P

P

E

P

R

H

A

A

E

E

Q

S

A

V

L

L

R

R

T

D

A

C

M

L

A

A

M

V

G

G

A

A

D

D

K

E

G

A

V

K

I

L

V

V

D

S

I

-

E

D

E

R

L

A

E

F

E

G

P
x
A

D
|
D

E
x
T

H

L

T

A

I
x
T

A

S

K

A

I

A

L

M

A

A

A

N

T

T

I

I

K

K

E

H

E

F

D

G

L

V

A

P

F

-

D

D

I

L

I

I

L

L

C
|
C

G
|
G

Y

R

M
x
Q

A
|
A

V

I

D

D

D
x
G

G

D

S
x
T

A
|
A

Q

Q

V
|
V

G

G

P

P

R

E

L

I

A

A

E

E

L

H

L

L

E

G

I

L

P

P

H

Q

I

V

S

T

T

A

I

A

T

L

K

K

L

V

D

Q

I

V

D

K

G

D

N

D

K

T

V

V

S

V

V

V

E

D

K

R

D

D

V

N

E

E

G

Q

D

M

V

S

E

M

L

T

I

F

D

T

S

M

S

K

L

M

P

P

I

C

L

V

V

V

T

T

A

V

Q

M

Q

R

G

S

L

-

N

K

D

D

P

L

R

R

Y

F

P

A

S

S

L

I

P

R

G

G

I

K

M

M

K

K

A

A

K

R

K

K

binding flavin-adenine dinucleotide: C6 (= C6), V7 (≠ L7), K8 (= K8), Y37 (= Y36), D38 (= D37), Y40 (= Y39), M61 (≠ I60), A92 (≠ P92), D93 (= D93), T94 (≠ E94), T97 (≠ I97), C116 (= C117), G117 (= G118), Q119 (≠ M120), A120 (= A121), G123 (≠ D124), T125 (≠ S126), A126 (= A127), V128 (= V129)

7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 97% coverage: 3:252/259 of query aligns to 4:258/265 of 7qh2B

query
sites
7qh2B

I

I

L

L

V

V

C
|
C

L

I

K
|
K

Q

Q

T

V

F

P

D

G

T

T

E

S

E

N

-

V

R

E

I

V

T

D

I

P

E

E

N

T

G

G

R

V

I

L

S

I

E

R

D

D

G

G

V

V

E

E

F

S

V

K

I

L

N

N

P

P

Y

Y

D
|
D

E

L

Y

F

A

G

V

L

E

E

E

T

A

A

I

F

R

R

L

L

K

K

E

E

K

Q

H

L

G

G

E

T

V

I

T

T

V

T

I

L

T

S

I
x
M

G

G

P

P

E

M

R

Q

A

S

E

K

Q

E

A

V

L

L

R

M

T

E

A

S

M

F

A

Y

M

M

G

G

A

A

D

D

K

E

G

G

V

C

I

L

V

L

D

S

I

D

E

R

E

K

L

F

E

G

E

A

P
x
D

D
x
V

E

V

H

A

T

T

I

S

A

Y

K

T

I
x
L

L

A

A

Q

A

G

T

T

I

K

K

R

E

L

E

G

D

D

L

-

A

-

F

F

D

D

I

L

I

I

L

I

C

C

G
|
G

Y

K

M
x
Q

A
x
T

V

T

D

D

D

G

G

D

S
x
T

A
|
A

Q

Q

V
|
V

G

G

P

P

R

E

L

M

A

A

E

E

L

F

L

L

E

G

I

I

P

P

H

H

I

V

S

T

T

N

I

V

T

I

K

K

-

I

L

L

D

A

I

A

D

D

G

E

N

K

K

G

V

L

S

T

V

L

E

Q

K

M

D

N

V

M

E

E

G

E

D

S

V

L

E

E

L

I

I

Q

D

R

S

V

S

P

L

Y

P

P

I

C

L

L

V

I

T

T

A

V

Q

D

Q

K

G

D

L

I

N

Y

D

T

P

P

R

R

Y

L

P

P

S

S

L

Y

P

K

G

R

I

K

M

L

K

D

A

I

K

S

K

K

K

N

P

P

-

E

-

I

-

K

-

I

L

L

T

T

R

L

L

K

E

D

L

M

D

Y

D

D

L

T

D

N

L

E

D

K

E

K

E

Y

E

G

I

L

A

S

A

G

K

S

T

P

E

T

T

Q

I

V

D

E

V

R

F

I

L

F

P

P

K

E

K

S

Q

N

A

V

G

E

K

K

I

T

-

S

L

F

Q

E

G

G

D

D

I

G

K

K

D

V

Q

L

A

A

K

K

E

A

L

L

V

L

Q

G

L

I

L

L

binding flavin-adenine dinucleotide: C7 (= C6), K9 (= K8), D39 (= D37), M62 (≠ I60), D94 (≠ P92), V95 (≠ D93), L102 (≠ I100), G118 (= G118), Q120 (≠ M120), T121 (≠ A121), T126 (≠ S126), A127 (= A127), V129 (= V129)

1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
32% identity, 99% coverage: 1:257/259 of query aligns to 1:261/261 of 1o96A

query
sites
1o96A

M

M

N

K

I

I

L

L

V

V

C
x
A

L
x
V

K
|
K

Q

Q

T

T

F

A

D

A

T

L

E

E

R

D

I

F

T

E

I

I

E

R

N

D

G

G

R

M

-

D

I

V

S

D

E

E

D

D

G

F

V

M

E

M

F

Y

V

D

I

L

N

N

P

E

Y

W

D
|
D

E

D

Y

F

A

S

V

L

E

E

A

A

I

M

R

K

L

I

K

K

E

E

K

S

H

S

G

D

G

T

E

D

V

V

T

E

V

V

I

V

T

V

I

V

-

S

-
x
V

G

G

P

P

E

D

R

R

A

V

E

D

Q

E

A

S

L

L

R

R

T

K

A

C

M

L

A

A

M

K

G

G

A

A

D

D

K

R

G

A

V

V

I

R

V

V

D

-

I

W

E

D

L

A

E

A

E

E

E

G

P

S

D

D

E

A

H

I

T

V

I
x
V

A

G

K

R

I

I

L

L

A

T

A

E

T

V

I

I

K

K

E

K

E

E

D

-

L

-

A

A

F

P

D

D

I

M

I

V

L

F

C
x
A

G
|
G

Y

V

M
x
Q

A
x
S

V

S

D

D

D

Q

G

A

S
x
Y

A
|
A

Q

S

V
x
T

G

G

P

I

R

S

L

V

A

A

E

S

L

Y

L

L

E

N

I

W

P

P

H

H

I

A

S

A

T

V

I

V

T

A

K

D

L

L

D

Q

I

Y

D

K

-

P

-

G

G

D

N

N

K

K

V

A

S

V

V

I

E

R

K

R

D

E

V

L

E

E

G

G

D

G

V

M

-

L

E

Q

L

E

I

V

D

E

S

I

S

N

L

C

P

P

I

A

L

V

V

L

T

T

A

I

Q
|
Q

Q

L

G

G

L

I

N

N

D

K

P

P

R

R

Y

Y

P

A

S

S

L

L

P

R

G

G

I

I

M

K

K

Q

A

A

K

A

K

T

K

K

P

P

L

I

T

E

R

E

L

V

E

S

L

L

D

A

D

D

L

I

D

G

L

L

D

S

E

A

E

N

E

D

I

V

A

G

A

A

K

A

T

Q

E

S

T

M

I

S

D

R

V

V

-

R

-

M

F

Y

L

I

P

P

P

E

K

K

K

G

Q

R

A

A

G

-

K

T

I

M

L

I

Q

E

G

G

D

T

I

I

K

S

D

E

Q

Q

A

A

K

A

E

K

L

I

V

I

Q

Q

L

I

R

-

N

N

E

E

A

F

K

K

binding adenosine monophosphate: A6 (≠ C6), V7 (≠ L7), K8 (= K8), D38 (= D37), V63 (vs. gap), V99 (≠ I97), A117 (≠ C117), G118 (= G118), Q120 (≠ M120), S121 (≠ A121), Y126 (≠ S126), A127 (= A127), T129 (≠ V129)
binding flavin-adenine dinucleotide: Q120 (≠ M120), Q182 (= Q179)

P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
33% identity, 99% coverage: 1:257/259 of query aligns to 1:262/264 of P53570

query
sites
P53570

M

M

N

K

I

I

L

L

V

V

C
x
A

L

V

K

K

Q

Q

T

T

F

A

D

A

T

L

E

E

R

D

I

F

T

E

I

I

-

R

E

E

N

D

G

G

R

M

-

D

I

V

S

D

E

E

D

D

G

F

V

M

E

M

F

Y

V

D

I

L

N
|
N

P
x
E

Y
x
W

D
|
D

E

D

Y

F

A

S

V

L

E

E

A

A

I

M

R

K

L

I

K

K

E

E

K

S

H

S

G

D

G

T

E

D

V

V

T

E

V

V

I

V

T

V

I

V

-

S

-

V

G

G

P

P

E

D

R

R

A

V

E

D

Q

E

A

S

L

L

R

R

T

K

A

C

M

L

A

A

M

K

G

G

A

A

D

D

K

R

G

A

V

V

I

R

V

V

D

-

I

W

E

D

L

A

E

A

E

E

E

G

P

S

D

D

E

A

H

I

T

V

I

V

A

G

K

R

I

I

L

L

A

T

A

E

T

V

I

I

K

K

E

K

E

E

D

-

L

-

A

A

F

P

D

D

I

M

I

V

L

F

C

A

G
|
G

Y
x
V

M
x
Q

A
x
S

V

S

D

D

D

Q

G

A

S
x
Y

A
|
A

Q
x
S

V
x
T

G

G

P

I

R

S

L

V

A

A

E

S

L

Y

L

L

E

N

I

W

P

P

H

H

I

A

S

A

T

V

I

V

T

A

K

D

L

L

D

Q

I

Y

D

K

-

P

-

G

G

D

N

N

K

K

V

A

S

V

V

I

E

R

K

R

D

E

V

L

E

E

G

G

D

G

V

M

-

L

E

Q

L

E

I

V

D

E

S

I

S

N

L

C

P

P

I

A

L

V

V

L

T

T

A

I

Q

Q

Q

L

G

G

L

I

N

N

D

K

P

P

R

R

Y

Y

P

A

S

S

L

L

P

R

G

G

I

I

M

K

K

Q

A

A

K

A

K

T

K

K

P

P

L

I

T

E

R

E

L

V

E

S

L

L

D

A

D

D

L

I

D

G

L

L

D

S

E

A

E

N

E

D

I

V

A

G

A

A

K

A

T

Q

E

S

T

M

I

S

D

R

V

V

-

R

-

M

F

Y

L

I

P

P

P

E

K

K

K

G

Q

R

A

A

G

-

K

T

I

M

L

I

Q

E

G

G

D

T

I

I

K

S

D

E

Q

Q

A

A

K

A

E

K

L

I

V

I

Q

Q

L

I

R

-

N

N

E

E

A

F

K

K

A6 (≠ C6) binding AMP
NEWD 36:39 (≠ NPYD 34:37) binding AMP
GVQS 119:122 (≠ GYMA 118:121) binding AMP
YAST 127:130 (≠ SAQV 126:129) binding AMP

7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
32% identity, 97% coverage: 2:253/259 of query aligns to 3:265/282 of 7koeA

query
sites
7koeA

N

N

I

V

L

V

V

V

C
|
C

L
x
I

K
|
K

Q

Q

T

V

F

P

D

D

T

T

E

T

E

N

-

V

R

R

I

I

T

D

I

R

E

K

N

T

G

N

R

N

I

L

S

V

E

R

D

E

G

G

V

V

E

P

F

S

V

I

I

I

N

N

P

P

Y

D

D
|
D

E

E

Y

R

A

A

V

L

E

E

E

L

A

A

I

S

R

Q

L

L

K

K

E

E

K

K

H

F

G

G

G

A

E

T

V

V

T

Y

V

V

I

I

T

T

I
x
M

G

G

P

P

E

P

R

Q

A

A

E

K

Q

E

A

A

L

L

R

K

T

D

A

A

M

I

A

A

M

F

G

G

A

L

D

D

K

E

G

A

V

V

I

H

V

L

D

S

I

-

E

D

E

R

L

T

E

F

E

A

E

G

P
x
A

D
|
D

E
x
T

H

L

T

A

I
x
T

A

T

K

Y

I

T

L
|
L

A

Y

A

W

T

G

I

I

K

K

-

K

-

I

E

E

D

R

L

I

A

G

-

K

F

I

D

D

I

L

I

I

L

L

C
x
T

G
|
G

Y

K

M

Q

A
|
A

V

V

D

D

D
x
G

G

D

S
x
T

A
x
G

Q

Q

V
|
V

G

G

P

P

R

G

L

L

A

A

E

T

L

R

L

F

E

G

I

Y

-

A

-

L

-

G

P

A

H

Y

I

V

S

V

T

R

I

I

T

E

K

E

L

I

D

D

I

P

D

E

G

K

N

K

K

E

V

M

S

V

V

I

E

V

K

R

D

R

V

L

E

D

G

Q

D

G

V

F

E

E

L

K

I

I

D

R

S

L

S

K

L

L

P

P

I

A

L

V

V

L

T

T

A

I

Q

T

Q

D

G

E

L

L

N

N

D

K

P

P

R

R

Y

Y

P

A

S

D

L

L

P

P

G

N

I

L

M

I

K

R

A

A

K

I

K

R

K

Y

P

E

L

P

T

I

R

V

L

W

E

T

L

H

D

K

D

D

L

L

D

G

L

L

D

D

E

P

E

K

-

C

-

G

-

F

I

F

A

G

A

S

K

P

T

T

E

R

T

V

I

V

D

S

V

T

F

N

L

I

P

P

K

A

K

R

Q

K

A

G

G

G

K

D

I

I

L

I

Q

S

G

K

-

N

-

E

D

D

I

P

K

E

D

V

Q

A

A

A

K

E

E

K

L

L

V

I

Q

E

L

A

L

L

R

K

binding flavin-adenine dinucleotide: C7 (= C6), I8 (≠ L7), K9 (= K8), D39 (= D37), M62 (≠ I60), A93 (≠ P92), D94 (= D93), T95 (≠ E94), T98 (≠ I97), L102 (= L101), T121 (≠ C117), G122 (= G118), A125 (= A121), G128 (≠ D124), T130 (≠ S126), G131 (≠ A127), V133 (= V129)

5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
34% identity, 67% coverage: 32:204/259 of query aligns to 34:202/251 of 5ow0B

query
sites
5ow0B

V

M

I

I

N

G

P
x
L

Y
|
Y

D
|
D

E

E

Y

N

A

A

V

V

E

E

E

L

A

G

I

V

R

Q

L

L

K

K

E

E

K

K

H

L

G

G

G

A

E

D

V

L

T

T

V

V

I

V

T

S

I
x
Y

G

G

P

R

E

N

R

D

A

D

E

V

Q

Q

A

F

L

L

R

R

T

K

A

A

M

L

A

A

M

M

G

G

A

A

D

D

K

K

G

V

V

V

I

L

V

V

D

E

I

-

E

-

L

-

E

G

E

D

E

S

P

D

D

D

E

P

H

Y

T

V

I
|
I

A

A

K

A

I

N

L

L

A

K

A

D

T

A

I

I

K

D

E

R

E

Q

D

G

L

-

A

T

F

V

D

D

I

L

I

I

L

L

C
x
A

G
|
G

Y

R

M
x
Q

A
x
S

V

S

D

D

D

M

G

D

S
x
R

A
x
G

Q
x
V

V
|
V

G
x
P

P

G

R

V

L

L

A

A

E

G

L

M

L

L

E

D

I

L

P

P

H

F

I

V

S

P

T

Q

I

A

T

C

K

S

L

V

D

E

-

S

I

V

D

D

G

G

N

G

K

W

V

K

S

I

V

S

E

Q

K

I

D

T

V

E

E

T

G

G

D

K

V

R

E

L

L

L

I

K

D

L

S

S

S

G

L

K

P

G

I

V

L

L

V

S

T

I

A

T

Q

S

Q

V

G

P

L

E

N

N

D

V

P

P

R

R

Y

I

P

P

S

A

L

V

P

K

G

A

I

I

M

F

K

A

A

A

K

K

P

P

L

V

T

E

R

K

L

L

binding adenosine monophosphate: D39 (= D37), Y62 (≠ I60), I95 (= I97), A114 (≠ C117), G115 (= G118), Q117 (≠ M120), S118 (≠ A121), R123 (≠ S126), G124 (≠ A127), V125 (≠ Q128), V126 (= V129), P127 (≠ G130)
binding flavin-adenine dinucleotide: L37 (≠ P35), Y38 (= Y36)

Sites not aligning to the query:

binding adenosine monophosphate: 6, 7, 8

Query Sequence

>WP_033102105.1 NCBI__GCF_000763315.1:WP_033102105.1
MNILVCLKQTFDTEERITIENGRISEDGVEFVINPYDEYAVEEAIRLKEKHGGEVTVITI
GPERAEQALRTAMAMGADKGVIVDIEELEEEPDEHTIAKILAATIKEEDLAFDIILCGYM
AVDDGSAQVGPRLAELLEIPHISTITKLDIDGNKVSVEKDVEGDVELIDSSLPILVTAQQ
GLNDPRYPSLPGIMKAKKKPLTRLELDDLDLDEEEIAAKTETIDVFLPPKKQAGKILQGD
IKDQAKELVQLLRNEAKVI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory