SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_033105871.1 NCBI__GCF_000763375.1:WP_033105871.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

A6NK06 Cis-aconitate decarboxylase; CAD; Aconitate decarboxylase; Aconitate decarboxylase 1; Cis-aconitic acid decarboxylase; Immune-responsive gene 1 protein; EC 4.1.1.6 from Homo sapiens (Human) (see paper)
24% identity, 81% coverage: 76:492/514 of query aligns to 59:453/481 of A6NK06

query
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D93 (= D111) mutation to A: Abolished cis-aconitate decarboxylase activity.
T97 (= T115) to M: strongly reduced cis-aconitate decarboxylase activity; dbSNP:rs767323284; mutation to A: Strongly reduced cis-aconitate decarboxylase activity.
H103 (= H123) mutation to A: Abolished cis-aconitate decarboxylase activity.
N152 (≠ I163) to S: increased cis-aconitate decarboxylase activity
H159 (= H168) to Q: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; to R: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1471882722; mutation to A: Abolished cis-aconitate decarboxylase activity.
K207 (≠ R213) mutation to A: Abolished cis-aconitate decarboxylase activity.
K272 (= K279) to Q: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1018207074; mutation to A: Abolished cis-aconitate decarboxylase activity.
R273 (= R280) to H: does not affect cis-aconitate decarboxylase activity
H277 (≠ E289) to Y: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs1289708092; mutation to A: Abolished cis-aconitate decarboxylase activity.
Y318 (= Y338) mutation to A: Abolished cis-aconitate decarboxylase activity.
R331 (≠ D360) to H: strongly reduced cis-aconitate decarboxylase activity; reduced protein stability; dbSNP:rs755737247

6r6uA Crystal structure of human cis-aconitate decarboxylase (see paper)
23% identity, 80% coverage: 83:492/514 of query aligns to 63:450/458 of 6r6uA

query
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binding (r,r)-2,3-butanediol: A148 (vs. gap), F155 (≠ K167), F242 (vs. gap), Y243 (vs. gap)

Query Sequence

>WP_033105871.1 NCBI__GCF_000763375.1:WP_033105871.1
MTVTHHVRVYKSEEDLPRENQLAWKIAEVATDAVEVEQDVVDMIINRIIDNASVAAASLT
RGPIIAARDQAFSHPVSTGGKGATVFGAPLDAHTSPEWAAWANGVAVRELDYHDTFLAAE
YSHPGDNIPPILAVAQHTGADGRALVRGIATGYEIQVDLVKAICLHKHKIDHVAHLGPSA
AAGIGTLLGLDAETIYQAVSQALHTTTATRQSRKGEISTWKAHAPAFAGKMAVEAVDRAM
RGQTSPSPIYEGEDGVIAWMLDGPDAAYEVPLPPKGEAKRAILDSYTKEHSAEYQAQAWI
DLARKLHKEYFSLPEPVEGRTWHDIESVVLHTSHHTHYVIGSGANDPQKYDPTASRETLD
HSIPYIFTVALQDGSWHHVDSYAPERAGRADTVELWNRVTTLEDAEWTRRYHSNDIAEKA
FGGRVEITFTDGSTLVDEIAVADAHPLGARPFARENYIAKFRLLAEPVLSSEEIERFLSL
VQRLPELTAAEVAELSIIAKPGVVASEPTTKGLF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory