SitesBLAST
Comparing WP_034209895.1 NCBI__GCF_000747155.1:WP_034209895.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
63% identity, 97% coverage: 9:396/399 of query aligns to 4:390/392 of P33221
- EL 22:23 (= EL 27:28) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E87) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R120) binding ATP
- K155 (= K161) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 166:171) binding ATP
- G162 (= G168) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ E185) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFI 201:204) binding ATP
- E203 (= E209) binding ATP
- E267 (= E273) binding Mg(2+)
- E279 (= E285) binding Mg(2+)
- D286 (= D292) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K361) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 368:369) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
62% identity, 97% coverage: 9:396/399 of query aligns to 3:387/389 of 1kjiA
- active site: E114 (= E121), K154 (= K161), S159 (= S166), G161 (= G168), E264 (= E273), E276 (= E285), D283 (= D292), T284 (= T293), R360 (= R369)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R120), I152 (≠ V159), K154 (= K161), S159 (= S166), S160 (= S167), G161 (= G168), Q164 (= Q171), E192 (= E201), V195 (≠ I204), E200 (= E209), Q222 (= Q231), E264 (= E273), F266 (= F275), E276 (= E285)
- binding magnesium ion: E264 (= E273), E276 (= E285)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
62% identity, 97% coverage: 9:396/399 of query aligns to 3:387/389 of 1ez1A
- active site: E114 (= E121), K154 (= K161), S159 (= S166), G161 (= G168), E264 (= E273), E276 (= E285), D283 (= D292), T284 (= T293), R360 (= R369)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R120), I152 (≠ V159), K154 (= K161), S159 (= S166), S160 (= S167), G161 (= G168), E192 (= E201), V194 (≠ F203), V195 (≠ I204), F197 (= F206), E200 (= E209), Q222 (= Q231), E264 (= E273), F266 (= F275), E276 (= E285)
- binding glycinamide ribonucleotide: G20 (= G26), E21 (= E27), L22 (= L28), E81 (= E87), I82 (= I88), S160 (= S167), D283 (= D292), K352 (= K361), R359 (= R368), R360 (= R369)
- binding magnesium ion: E264 (= E273), E276 (= E285)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
62% identity, 97% coverage: 9:396/399 of query aligns to 3:387/389 of 1eyzA
- active site: E114 (= E121), K154 (= K161), S159 (= S166), G161 (= G168), E264 (= E273), E276 (= E285), D283 (= D292), T284 (= T293), R360 (= R369)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R120), I152 (≠ V159), K154 (= K161), S159 (= S166), S160 (= S167), G161 (= G168), Q164 (= Q171), E192 (= E201), V195 (≠ I204), F197 (= F206), E200 (= E209), E264 (= E273), F266 (= F275), E276 (= E285)
- binding magnesium ion: E264 (= E273), E276 (= E285)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
62% identity, 97% coverage: 9:396/399 of query aligns to 3:386/388 of 1kjqA
- active site: E114 (= E121), K154 (= K161), E263 (= E273), E275 (= E285), D282 (= D292), T283 (= T293), R359 (= R369)
- binding adenosine-5'-diphosphate: R113 (= R120), I152 (≠ V159), K154 (= K161), E191 (= E201), V193 (≠ F203), V194 (≠ I204), F196 (= F206), E199 (= E209), Q221 (= Q231), F265 (= F275), E275 (= E285)
- binding magnesium ion: E263 (= E273), E275 (= E285)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
62% identity, 97% coverage: 9:396/399 of query aligns to 3:384/386 of 1kjjA
- active site: E114 (= E121), K154 (= K161), S159 (= S166), G161 (= G168), E261 (= E273), E273 (= E285), D280 (= D292), T281 (= T293), R357 (= R369)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R120), I152 (≠ V159), K154 (= K161), S159 (= S166), S160 (= S167), G161 (= G168), Q164 (= Q171), E189 (= E201), V192 (≠ I204), E197 (= E209), Q219 (= Q231), E261 (= E273), F263 (= F275), E273 (= E285)
- binding magnesium ion: E261 (= E273), E273 (= E285)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
62% identity, 97% coverage: 9:396/399 of query aligns to 3:384/386 of 1kj8A
- active site: E114 (= E121), K154 (= K161), S159 (= S166), G161 (= G168), E261 (= E273), E273 (= E285), D280 (= D292), T281 (= T293), R357 (= R369)
- binding adenosine-5'-triphosphate: R113 (= R120), I152 (≠ V159), K154 (= K161), S159 (= S166), S160 (= S167), G161 (= G168), Q164 (= Q171), E189 (= E201), V192 (≠ I204), F194 (= F206), E197 (= E209), Q219 (= Q231), G222 (= G234), E261 (= E273), F263 (= F275), E273 (= E285)
- binding glycinamide ribonucleotide: G20 (= G26), E21 (= E27), L22 (= L28), E81 (= E87), I82 (= I88), S160 (= S167), D280 (= D292), K349 (= K361), R356 (= R368)
- binding magnesium ion: E261 (= E273), E273 (= E285)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
49% identity, 98% coverage: 9:399/399 of query aligns to 8:413/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
47% identity, 98% coverage: 9:399/399 of query aligns to 10:402/409 of 2dwcB
- active site: E265 (= E273), E277 (= E285), D284 (= D292), T285 (= T293), R372 (= R369)
- binding adenosine-5'-diphosphate: R120 (= R120), H159 (≠ V159), K161 (= K161), H190 (≠ F203), I191 (= I204), F193 (= F206), E196 (= E209), F267 (= F275), E277 (= E285)
3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 91% coverage: 27:388/399 of query aligns to 17:366/380 of 3v4sA
- binding adenosine-5'-diphosphate: R106 (= R120), K146 (= K161), Y152 (vs. gap), G154 (= G168), Q157 (= Q171), W183 (≠ F203), V184 (≠ I204), E189 (= E209), N215 (≠ G234), F256 (= F275), N266 (≠ S284), E267 (= E285)
- binding carbonate ion: R271 (= R289), H273 (= H291), N274 (≠ D292)
3r5hA Crystal structure of adp-air complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 91% coverage: 27:388/399 of query aligns to 18:367/383 of 3r5hA
- binding adenosine-5'-diphosphate: R107 (= R120), K147 (= K161), Q158 (= Q171), W184 (≠ F203), V185 (≠ I204), F187 (= F206), E190 (= E209), N216 (≠ G234), F257 (= F275), N267 (≠ S284), E268 (= E285)
- binding 5-aminoimidazole ribonucleotide: Q18 (≠ E27), L19 (= L28), E76 (= E87), Y153 (vs. gap), R272 (= R289), K340 (= K361), R347 (= R368)
Sites not aligning to the query:
4dlkA Crystal structure of atp-ca++ complex of purk: n5- carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 91% coverage: 27:388/399 of query aligns to 18:367/380 of 4dlkA